The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy
Апстракт
The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.
Кључне речи:
polyphosphates / Phycomyces / P-31 NMR / anoxiaИзвор:
Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 2005, 1048, 482-486Издавач:
- New York Acad Sciences, New York
DOI: 10.1196/annals.1342.073
ISSN: 0077-8923
PubMed: 16154982
WoS: 000233712300066
Scopus: 2-s2.0-25144468817
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Zakrzewska, Joanna AU - Žižić, Milan AU - Zivić, Miroslav PY - 2005 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/139 AB - The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio. PB - New York Acad Sciences, New York T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus T1 - The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy EP - 486 SP - 482 VL - 1048 DO - 10.1196/annals.1342.073 ER -
@article{ author = "Zakrzewska, Joanna and Žižić, Milan and Zivić, Miroslav", year = "2005", abstract = "The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.", publisher = "New York Acad Sciences, New York", journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus", title = "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy", pages = "486-482", volume = "1048", doi = "10.1196/annals.1342.073" }
Zakrzewska, J., Žižić, M.,& Zivić, M.. (2005). The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus New York Acad Sciences, New York., 1048, 482-486. https://doi.org/10.1196/annals.1342.073
Zakrzewska J, Žižić M, Zivić M. The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:482-486. doi:10.1196/annals.1342.073 .
Zakrzewska, Joanna, Žižić, Milan, Zivić, Miroslav, "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):482-486, https://doi.org/10.1196/annals.1342.073 . .