TY  - JOUR
AU  - Cvetić-Antić, Tijana
AU  - Janosević, Dusica
AU  - Maksimović, Vuk
AU  - Zivić, Miroslav
AU  - Budimir, Snežana M
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
ER  - 

@article{
author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086"
}

Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 244.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244.
doi:10.1016/j.jplph.2019.153086 .

Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020),
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Calic, Dusica D
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1343
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control
EP  - 11967
IS  - 11
SP  - 11958
VL  - 27
DO  - 10.1007/s11356-020-07825-1
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Calic, Dusica D",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control",
pages = "11967-11958",
number = "11",
volume = "27",
doi = "10.1007/s11356-020-07825-1"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Calic, D. D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(11), 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Calic DD. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research. 2020;27(11):11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Calic, Dusica D, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control" in Environmental Science and Pollution Research, 27, no. 11 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Mitrović, Aleksandra Lj.
AU  - Bogdanović Pristov, Jelena
AU  - Radotić, Ksenija
AU  - Janošević, Dušica
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1816
AB  - Dioscorea balcanica Košanin, family Dioscoreaceae, is an endemic,
endangered liana species and a Tertiary relict of Balkan Peninsula. Gelatinous fibers,
specialized sclerenchyma cells and well-known components of tension wood, are
responsible for stem twining in liana plants. The aim of this investigation was to
determine changes in sclerenchyma cell walls correlated with the twining process in
liana plants. Our results showed that the differences in cell walls of sclerenchyma cells
between straight and twisted internodes are not visible by light microscopy, but they
are visible by SEM microscopy. In twisted internodes, cell walls of sclerenchyma cells
in the region of contact with the support (‘‘tension’’ side of twisted internodes) appear
smooth on the cross sections, suggesting cellulose microfibrils oriented almost
parallel to the fiber axis. In contrast, cell walls of the entire ring of sclerenchyma cells
in straight internodes, as well as cell walls of sclerenchyma cells on the “opposite”
side of twisted internodes, appear jagged on the cross sections, suggesting cellulose
microfibrils oriented at very high microfibril angle to the fiber axis. This result
suggests that gelatinous fibers in D. balcanica stem have lignified G-layer, and thus
could contribute to the understanding of ‘‘late lignification of the G-layer’’.
PB  - Institute for Nature Conservation of Serbia (Beograd)
PB  - Faculty of Sciences and Mathematics (Niš). Depattment of Biology and Ecology
C3  - 13th Symphosium on the flora of Southeastern Serbia and Neighboring Regions
T1  - Changes in sclerenchyma cell walls related to stem twining in Dioscorea balcanica
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1816
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Mitrović, Aleksandra Lj. and Bogdanović Pristov, Jelena and Radotić, Ksenija and Janošević, Dušica",
year = "2019",
abstract = "Dioscorea balcanica Košanin, family Dioscoreaceae, is an endemic,
endangered liana species and a Tertiary relict of Balkan Peninsula. Gelatinous fibers,
specialized sclerenchyma cells and well-known components of tension wood, are
responsible for stem twining in liana plants. The aim of this investigation was to
determine changes in sclerenchyma cell walls correlated with the twining process in
liana plants. Our results showed that the differences in cell walls of sclerenchyma cells
between straight and twisted internodes are not visible by light microscopy, but they
are visible by SEM microscopy. In twisted internodes, cell walls of sclerenchyma cells
in the region of contact with the support (‘‘tension’’ side of twisted internodes) appear
smooth on the cross sections, suggesting cellulose microfibrils oriented almost
parallel to the fiber axis. In contrast, cell walls of the entire ring of sclerenchyma cells
in straight internodes, as well as cell walls of sclerenchyma cells on the “opposite”
side of twisted internodes, appear jagged on the cross sections, suggesting cellulose
microfibrils oriented at very high microfibril angle to the fiber axis. This result
suggests that gelatinous fibers in D. balcanica stem have lignified G-layer, and thus
could contribute to the understanding of ‘‘late lignification of the G-layer’’.",
publisher = "Institute for Nature Conservation of Serbia (Beograd), Faculty of Sciences and Mathematics (Niš). Depattment of Biology and Ecology",
journal = "13th Symphosium on the flora of Southeastern Serbia and Neighboring Regions",
title = "Changes in sclerenchyma cell walls related to stem twining in Dioscorea balcanica",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1816"
}

Simonović Radosavljević, J., Mitrović, A. Lj., Bogdanović Pristov, J., Radotić, K.,& Janošević, D.. (2019). Changes in sclerenchyma cell walls related to stem twining in Dioscorea balcanica. in 13th Symphosium on the flora of Southeastern Serbia and Neighboring Regions
Institute for Nature Conservation of Serbia (Beograd)., 109.
https://hdl.handle.net/21.15107/rcub_rimsi_1816

Simonović Radosavljević J, Mitrović AL, Bogdanović Pristov J, Radotić K, Janošević D. Changes in sclerenchyma cell walls related to stem twining in Dioscorea balcanica. in 13th Symphosium on the flora of Southeastern Serbia and Neighboring Regions. 2019;:109.
https://hdl.handle.net/21.15107/rcub_rimsi_1816 .

Simonović Radosavljević, Jasna, Mitrović, Aleksandra Lj., Bogdanović Pristov, Jelena, Radotić, Ksenija, Janošević, Dušica, "Changes in sclerenchyma cell walls related to stem twining in Dioscorea balcanica" in 13th Symphosium on the flora of Southeastern Serbia and Neighboring Regions (2019):109,
https://hdl.handle.net/21.15107/rcub_rimsi_1816 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Misic, Danijela
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1244
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
EP  - 94
SP  - 87
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Misic, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
pages = "94-87",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Misic, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Misic D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Misic, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .

TY  - CONF
AU  - Mitrović, Aleksandra Lj.
AU  - Janošević, Dušica
AU  - Budimir, Snežana
AU  - Radotić, Ksenija
AU  - Donaldson, Lloyd
AU  - Spasić, Slađana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1821
AB  - Fractals are self-similar patterns, from exactly the same to nearly the same, and hence they are
common in Nature. Fractal analysis of digital images or signals already has the application in the
assessment of properties of tumors, viral infections, blood vessels, neurons, EEG signals, heart rate
variability, etc. In different fields of science, especially in botany, application of different fractal
methods is necessary to obtain a complete picture of structures or processes.
We suggest a new method for 2D Higuchi’s fractal dimension estimation for use in plant analytical microscopy. To present its performance, we used two different sets of digital microscopic
images: light microscopy micrographs collected during Tacitus bellus direct shoot organogenesis
from leaf explants in vitro, and confocal laser scanning microscopy images of stem cross sections
of juvenile Picea omorika trees exposed to static bending stress. Estimated Higuchi’s fractal dimension of presented sets of micrographs enables quantification, separation and alignment of subsequent morphogenic stages of shoot organogenesis on the time scale, i.e. quantitative gradation of
structural changes of wood cell properties on a compression severity scale, respectively.
Suggested fractal analysis method, combined with statistical analysis, could be used for quantification of structure complexity that characterizes cells and tissues during different growth and
developmental processes or stress related structural changes in plants, as well as for the evaluation of the synchronization of those processes. It allows fast computational analysis of micrographs and is independent of the type of microscopy used.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Higuchi’s fractal dimension in plant histology
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1821
ER  - 

@conference{
author = "Mitrović, Aleksandra Lj. and Janošević, Dušica and Budimir, Snežana and Radotić, Ksenija and Donaldson, Lloyd and Spasić, Slađana",
year = "2018",
abstract = "Fractals are self-similar patterns, from exactly the same to nearly the same, and hence they are
common in Nature. Fractal analysis of digital images or signals already has the application in the
assessment of properties of tumors, viral infections, blood vessels, neurons, EEG signals, heart rate
variability, etc. In different fields of science, especially in botany, application of different fractal
methods is necessary to obtain a complete picture of structures or processes.
We suggest a new method for 2D Higuchi’s fractal dimension estimation for use in plant analytical microscopy. To present its performance, we used two different sets of digital microscopic
images: light microscopy micrographs collected during Tacitus bellus direct shoot organogenesis
from leaf explants in vitro, and confocal laser scanning microscopy images of stem cross sections
of juvenile Picea omorika trees exposed to static bending stress. Estimated Higuchi’s fractal dimension of presented sets of micrographs enables quantification, separation and alignment of subsequent morphogenic stages of shoot organogenesis on the time scale, i.e. quantitative gradation of
structural changes of wood cell properties on a compression severity scale, respectively.
Suggested fractal analysis method, combined with statistical analysis, could be used for quantification of structure complexity that characterizes cells and tissues during different growth and
developmental processes or stress related structural changes in plants, as well as for the evaluation of the synchronization of those processes. It allows fast computational analysis of micrographs and is independent of the type of microscopy used.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Higuchi’s fractal dimension in plant histology",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1821"
}

Mitrović, A. Lj., Janošević, D., Budimir, S., Radotić, K., Donaldson, L.,& Spasić, S.. (2018). Higuchi’s fractal dimension in plant histology. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 20.
https://hdl.handle.net/21.15107/rcub_rimsi_1821

Mitrović AL, Janošević D, Budimir S, Radotić K, Donaldson L, Spasić S. Higuchi’s fractal dimension in plant histology. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:20.
https://hdl.handle.net/21.15107/rcub_rimsi_1821 .

Mitrović, Aleksandra Lj., Janošević, Dušica, Budimir, Snežana, Radotić, Ksenija, Donaldson, Lloyd, Spasić, Slađana, "Higuchi’s fractal dimension in plant histology" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):20,
https://hdl.handle.net/21.15107/rcub_rimsi_1821 .

TY  - CONF
AU  - Trailović, Maja
AU  - Dragićević, Ivana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2436
AB  - Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK).1 SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.
PB  - Srpsko biološko društvo
C3  - DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
T1  - Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2436
ER  - 

@conference{
author = "Trailović, Maja and Dragićević, Ivana and Raspor, Martin and Motyka, Vaclav and Ninković, Slavica",
year = "2018",
abstract = "Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK).1 SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.",
publisher = "Srpsko biološko društvo",
journal = "DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.",
title = "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2436"
}

Trailović, M., Dragićević, I., Raspor, M., Motyka, V.,& Ninković, S.. (2018). Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
Srpsko biološko društvo..
https://hdl.handle.net/21.15107/rcub_rimsi_2436

Trailović M, Dragićević I, Raspor M, Motyka V, Ninković S. Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2436 .

Trailović, Maja, Dragićević, Ivana, Raspor, Martin, Motyka, Vaclav, Ninković, Slavica, "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro" in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018. (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2436 .

TY  - CONF
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Savić, Jelena
AU  - Belic, Maja
AU  - Ćosić, Tatjana
AU  - Calic, Dusica D
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2458
AB  - Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2458
ER  - 

@conference{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Kostić, Miroslav and Savić, Jelena and Belic, Maja and Ćosić, Tatjana and Calic, Dusica D",
year = "2018",
abstract = "Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2458"
}

Devrnja, N., Kostić, I., Lazarević, J., Kostić, M., Savić, J., Belic, M., Ćosić, T.,& Calic, D. D.. (2018). Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_rimsi_2458

Devrnja N, Kostić I, Lazarević J, Kostić M, Savić J, Belic M, Ćosić T, Calic DD. Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2458 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Kostić, Miroslav, Savić, Jelena, Belic, Maja, Ćosić, Tatjana, Calic, Dusica D, "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2458 .

TY  - JOUR
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Mitrović, Aleksandra Lj.
AU  - Steinbach, Gabor
AU  - Mouille, Gregory
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Janosević, Dusica
AU  - Vuković, Marina
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1057
AB  - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Parenchyma cell wall structure in twining stem of Dioscorea balcanica
EP  - 4669
IS  - 11
SP  - 4653
VL  - 24
DO  - 10.1007/s10570-017-1460-1
ER  - 

@article{
author = "Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Mitrović, Aleksandra Lj. and Steinbach, Gabor and Mouille, Gregory and Tufegdžić, Srđan J. and Maksimović, Vuk and Mutavdžić, Dragosav and Janosević, Dusica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2017",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Parenchyma cell wall structure in twining stem of Dioscorea balcanica",
pages = "4669-4653",
number = "11",
volume = "24",
doi = "10.1007/s10570-017-1460-1"
}

Simonović Radosavljević, J., Bogdanović Pristov, J., Mitrović, A. Lj., Steinbach, G., Mouille, G., Tufegdžić, S. J., Maksimović, V., Mutavdžić, D., Janosević, D., Vuković, M., Garab, G.,& Radotić, K.. (2017). Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose
Springer, Dordrecht., 24(11), 4653-4669.
https://doi.org/10.1007/s10570-017-1460-1

Simonović Radosavljević J, Bogdanović Pristov J, Mitrović AL, Steinbach G, Mouille G, Tufegdžić SJ, Maksimović V, Mutavdžić D, Janosević D, Vuković M, Garab G, Radotić K. Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose. 2017;24(11):4653-4669.
doi:10.1007/s10570-017-1460-1 .

Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Mitrović, Aleksandra Lj., Steinbach, Gabor, Mouille, Gregory, Tufegdžić, Srđan J., Maksimović, Vuk, Mutavdžić, Dragosav, Janosević, Dusica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Parenchyma cell wall structure in twining stem of Dioscorea balcanica" in Cellulose, 24, no. 11 (2017):4653-4669,
https://doi.org/10.1007/s10570-017-1460-1 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Kostić, Igor
AU  - Kostic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/691
AB  - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
EP  - 122
SP  - 118
VL  - 155
DO  - 10.1016/j.scienta.2013.03.017
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Kostić, Igor and Kostic, Miroslav",
year = "2013",
abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca",
pages = "122-118",
volume = "155",
doi = "10.1016/j.scienta.2013.03.017"
}

Calic, D. D., Devrnja, N. M., Kostić, I.,& Kostic, M.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae
Elsevier, Amsterdam., 155, 118-122.
https://doi.org/10.1016/j.scienta.2013.03.017

Calic DD, Devrnja NM, Kostić I, Kostic M. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155:118-122.
doi:10.1016/j.scienta.2013.03.017 .

Calic, Dusica D, Devrnja, Nina M, Kostić, Igor, Kostic, Miroslav, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155 (2013):118-122,
https://doi.org/10.1016/j.scienta.2013.03.017 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Bohanec, Borut
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Tubic, Ljiljana
AU  - Kostić, Igor
AU  - Zdravkovic-Korac, Snežana R
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/701
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
EP  - 762
IS  - 3
SP  - 755
VL  - 27
DO  - 10.1007/s00468-012-0830-4
ER  - 

@article{
author = "Calic, Dusica D and Bohanec, Borut and Devrnja, Nina M and Milojević, Jelena D and Tubic, Ljiljana and Kostić, Igor and Zdravkovic-Korac, Snežana R",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
pages = "762-755",
number = "3",
volume = "27",
doi = "10.1007/s00468-012-0830-4"
}

Calic, D. D., Bohanec, B., Devrnja, N. M., Milojević, J. D., Tubic, L., Kostić, I.,& Zdravkovic-Korac, S. R.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 27(3), 755-762.
https://doi.org/10.1007/s00468-012-0830-4

Calic DD, Bohanec B, Devrnja NM, Milojević JD, Tubic L, Kostić I, Zdravkovic-Korac SR. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):755-762.
doi:10.1007/s00468-012-0830-4 .

Calic, Dusica D, Bohanec, Borut, Devrnja, Nina M, Milojević, Jelena D, Tubic, Ljiljana, Kostić, Igor, Zdravkovic-Korac, Snežana R, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):755-762,
https://doi.org/10.1007/s00468-012-0830-4 . .

TY  - CONF
AU  - Spasić, Slađana
AU  - Savić, A.
AU  - Nikolic, L.
AU  - Budimir, Snežana M
AU  - Janosević, D.
AU  - Mitrović, Aleksandra Lj.
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/561
AB  - Significant contribution to understanding of complex biological systems emerged from application of fractal analysis. This paper presents a fractal analysis as one of many mathematical methods in the analysis of biological signals. Higuchi's fractal dimension is used in stationary and quasi-stationary bio-signals (EEG, ECoG) analysis and appropriately modified in the analysis of the bimodal pattern of neuronal activity as non-stationary signals.
C3  - 2012 20th Telecommunications Forum, TELFOR 2012 - Proceedings
T1  - Applications of Higuchi's fractal dimension in the Analysis of Biological Signals
EP  - 641
SP  - 639
DO  - 10.1109/TELFOR.2012.6419291
ER  - 

@conference{
author = "Spasić, Slađana and Savić, A. and Nikolic, L. and Budimir, Snežana M and Janosević, D. and Mitrović, Aleksandra Lj.",
year = "2012",
abstract = "Significant contribution to understanding of complex biological systems emerged from application of fractal analysis. This paper presents a fractal analysis as one of many mathematical methods in the analysis of biological signals. Higuchi's fractal dimension is used in stationary and quasi-stationary bio-signals (EEG, ECoG) analysis and appropriately modified in the analysis of the bimodal pattern of neuronal activity as non-stationary signals.",
journal = "2012 20th Telecommunications Forum, TELFOR 2012 - Proceedings",
title = "Applications of Higuchi's fractal dimension in the Analysis of Biological Signals",
pages = "641-639",
doi = "10.1109/TELFOR.2012.6419291"
}

Spasić, S., Savić, A., Nikolic, L., Budimir, S. M., Janosević, D.,& Mitrović, A. Lj.. (2012). Applications of Higuchi's fractal dimension in the Analysis of Biological Signals. in 2012 20th Telecommunications Forum, TELFOR 2012 - Proceedings, 639-641.
https://doi.org/10.1109/TELFOR.2012.6419291

Spasić S, Savić A, Nikolic L, Budimir SM, Janosević D, Mitrović AL. Applications of Higuchi's fractal dimension in the Analysis of Biological Signals. in 2012 20th Telecommunications Forum, TELFOR 2012 - Proceedings. 2012;:639-641.
doi:10.1109/TELFOR.2012.6419291 .

Spasić, Slađana, Savić, A., Nikolic, L., Budimir, Snežana M, Janosević, D., Mitrović, Aleksandra Lj., "Applications of Higuchi's fractal dimension in the Analysis of Biological Signals" in 2012 20th Telecommunications Forum, TELFOR 2012 - Proceedings (2012):639-641,
https://doi.org/10.1109/TELFOR.2012.6419291 . .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Janosević, Dusica A
AU  - Budimir, Snežana M
AU  - Bogdanović Pristov, Jelena
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/533
AB  - Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis
EP  - 361
IS  - 2
SP  - 357
VL  - 56
DO  - 10.1007/s10535-012-0098-y
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Janosević, Dusica A and Budimir, Snežana M and Bogdanović Pristov, Jelena",
year = "2012",
abstract = "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis",
pages = "361-357",
number = "2",
volume = "56",
doi = "10.1007/s10535-012-0098-y"
}

Mitrović, A. Lj., Janosević, D. A., Budimir, S. M.,& Bogdanović Pristov, J.. (2012). Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 56(2), 357-361.
https://doi.org/10.1007/s10535-012-0098-y

Mitrović AL, Janosević DA, Budimir SM, Bogdanović Pristov J. Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. in Biologia Plantarum. 2012;56(2):357-361.
doi:10.1007/s10535-012-0098-y .

Mitrović, Aleksandra Lj., Janosević, Dusica A, Budimir, Snežana M, Bogdanović Pristov, Jelena, "Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis" in Biologia Plantarum, 56, no. 2 (2012):357-361,
https://doi.org/10.1007/s10535-012-0098-y . .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Milojević, Jelena D
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Ćalić, Dušica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2887
AB  - Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
T1  - Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots
EP  - 396
SP  - 389
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2887
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Milojević, Jelena D and Devrnja, Nina and Kostić, Igor and Ćalić, Dušica",
year = "2012",
abstract = "Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.",
title = "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots",
pages = "396-389",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2887"
}

Zdravković-Korać, S., Tubić, L., Milojević, J. D., Devrnja, N., Kostić, I.,& Ćalić, D.. (2012). Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
Belgrade: Institute of Forestry., 389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887

Zdravković-Korać S, Tubić L, Milojević JD, Devrnja N, Kostić I, Ćalić D. Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.. 2012;:389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Milojević, Jelena D, Devrnja, Nina, Kostić, Igor, Ćalić, Dušica, "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots" in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. (2012):389-396,
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .

TY  - JOUR
AU  - Mitic, Nevena V
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravkovic-Korac, Snežana R
AU  - Nikolic, Radomirka R
AU  - Raspor, Martin
AU  - Đorđević, Tatjana
AU  - Maksimović, Vuk
AU  - Živković, Suzana
AU  - Krstić-Milošević, Dijana B.
AU  - Stanisic, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/599
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
EP  - 1211
IS  - 12
SP  - 1203
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
ER  - 

@article{
author = "Mitic, Nevena V and Dmitrović, Slavica and Đorđević, Mirka and Zdravkovic-Korac, Snežana R and Nikolic, Radomirka R and Raspor, Martin and Đorđević, Tatjana and Maksimović, Vuk and Živković, Suzana and Krstić-Milošević, Dijana B. and Stanisic, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
pages = "1211-1203",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009"
}

Mitic, N. V., Dmitrović, S., Đorđević, M., Zdravkovic-Korac, S. R., Nikolic, R. R., Raspor, M., Đorđević, T., Maksimović, V., Živković, S., Krstić-Milošević, D. B., Stanisic, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 169(12), 1203-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Mitic NV, Dmitrović S, Đorđević M, Zdravkovic-Korac SR, Nikolic RR, Raspor M, Đorđević T, Maksimović V, Živković S, Krstić-Milošević DB, Stanisic M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):1203-1211.
doi:10.1016/j.jplph.2012.04.009 .

Mitic, Nevena V, Dmitrović, Slavica, Đorđević, Mirka, Zdravkovic-Korac, Snežana R, Nikolic, Radomirka R, Raspor, Martin, Đorđević, Tatjana, Maksimović, Vuk, Živković, Suzana, Krstić-Milošević, Dijana B., Stanisic, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):1203-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Kostić, Igor
AU  - Janosević, Dusica A
AU  - Budimir, Snežana M
AU  - Zdravkovic-Korac, Snežana R
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/583
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
EP  - 1744
IS  - 12
SP  - 1741
VL  - 47
DO  - 10.21273/HORTSCI.47.12.1741
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Milojević, Jelena D and Kostić, Igor and Janosević, Dusica A and Budimir, Snežana M and Zdravkovic-Korac, Snežana R",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
pages = "1744-1741",
number = "12",
volume = "47",
doi = "10.21273/HORTSCI.47.12.1741"
}

Calic, D. D., Devrnja, N. M., Milojević, J. D., Kostić, I., Janosević, D. A., Budimir, S. M.,& Zdravkovic-Korac, S. R.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience
Amer Soc Horticultural Science, Alexandria., 47(12), 1741-1744.
https://doi.org/10.21273/HORTSCI.47.12.1741

Calic DD, Devrnja NM, Milojević JD, Kostić I, Janosević DA, Budimir SM, Zdravkovic-Korac SR. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):1741-1744.
doi:10.21273/HORTSCI.47.12.1741 .

Calic, Dusica D, Devrnja, Nina M, Milojević, Jelena D, Kostić, Igor, Janosević, Dusica A, Budimir, Snežana M, Zdravkovic-Korac, Snežana R, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):1741-1744,
https://doi.org/10.21273/HORTSCI.47.12.1741 . .