TY  - JOUR
AU  - Calic, Dusica D
AU  - Bohanec, Borut
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Tubic, Ljiljana
AU  - Kostić, Igor
AU  - Zdravkovic-Korac, Snežana R
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/701
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
EP  - 762
IS  - 3
SP  - 755
VL  - 27
DO  - 10.1007/s00468-012-0830-4
ER  - 

@article{
author = "Calic, Dusica D and Bohanec, Borut and Devrnja, Nina M and Milojević, Jelena D and Tubic, Ljiljana and Kostić, Igor and Zdravkovic-Korac, Snežana R",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
pages = "762-755",
number = "3",
volume = "27",
doi = "10.1007/s00468-012-0830-4"
}

Calic, D. D., Bohanec, B., Devrnja, N. M., Milojević, J. D., Tubic, L., Kostić, I.,& Zdravkovic-Korac, S. R.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 27(3), 755-762.
https://doi.org/10.1007/s00468-012-0830-4

Calic DD, Bohanec B, Devrnja NM, Milojević JD, Tubic L, Kostić I, Zdravkovic-Korac SR. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):755-762.
doi:10.1007/s00468-012-0830-4 .

Calic, Dusica D, Bohanec, Borut, Devrnja, Nina M, Milojević, Jelena D, Tubic, Ljiljana, Kostić, Igor, Zdravkovic-Korac, Snežana R, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):755-762,
https://doi.org/10.1007/s00468-012-0830-4 . .

TY  - JOUR
AU  - Mitic, Nevena V
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravkovic-Korac, Snežana R
AU  - Nikolic, Radomirka R
AU  - Raspor, Martin
AU  - Đorđević, Tatjana
AU  - Maksimović, Vuk
AU  - Živković, Suzana
AU  - Krstić-Milošević, Dijana B.
AU  - Stanisic, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/599
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
EP  - 1211
IS  - 12
SP  - 1203
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
ER  - 

@article{
author = "Mitic, Nevena V and Dmitrović, Slavica and Đorđević, Mirka and Zdravkovic-Korac, Snežana R and Nikolic, Radomirka R and Raspor, Martin and Đorđević, Tatjana and Maksimović, Vuk and Živković, Suzana and Krstić-Milošević, Dijana B. and Stanisic, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
pages = "1211-1203",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009"
}

Mitic, N. V., Dmitrović, S., Đorđević, M., Zdravkovic-Korac, S. R., Nikolic, R. R., Raspor, M., Đorđević, T., Maksimović, V., Živković, S., Krstić-Milošević, D. B., Stanisic, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 169(12), 1203-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Mitic NV, Dmitrović S, Đorđević M, Zdravkovic-Korac SR, Nikolic RR, Raspor M, Đorđević T, Maksimović V, Živković S, Krstić-Milošević DB, Stanisic M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):1203-1211.
doi:10.1016/j.jplph.2012.04.009 .

Mitic, Nevena V, Dmitrović, Slavica, Đorđević, Mirka, Zdravkovic-Korac, Snežana R, Nikolic, Radomirka R, Raspor, Martin, Đorđević, Tatjana, Maksimović, Vuk, Živković, Suzana, Krstić-Milošević, Dijana B., Stanisic, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):1203-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Kostić, Igor
AU  - Janosević, Dusica A
AU  - Budimir, Snežana M
AU  - Zdravkovic-Korac, Snežana R
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/583
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
EP  - 1744
IS  - 12
SP  - 1741
VL  - 47
DO  - 10.21273/HORTSCI.47.12.1741
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Milojević, Jelena D and Kostić, Igor and Janosević, Dusica A and Budimir, Snežana M and Zdravkovic-Korac, Snežana R",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
pages = "1744-1741",
number = "12",
volume = "47",
doi = "10.21273/HORTSCI.47.12.1741"
}

Calic, D. D., Devrnja, N. M., Milojević, J. D., Kostić, I., Janosević, D. A., Budimir, S. M.,& Zdravkovic-Korac, S. R.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience
Amer Soc Horticultural Science, Alexandria., 47(12), 1741-1744.
https://doi.org/10.21273/HORTSCI.47.12.1741

Calic DD, Devrnja NM, Milojević JD, Kostić I, Janosević DA, Budimir SM, Zdravkovic-Korac SR. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):1741-1744.
doi:10.21273/HORTSCI.47.12.1741 .

Calic, Dusica D, Devrnja, Nina M, Milojević, Jelena D, Kostić, Igor, Janosević, Dusica A, Budimir, Snežana M, Zdravkovic-Korac, Snežana R, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):1741-1744,
https://doi.org/10.21273/HORTSCI.47.12.1741 . .