TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Milojević, Jelena D
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Ćalić, Dušica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2887
AB  - Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
T1  - Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots
EP  - 396
SP  - 389
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2887
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Milojević, Jelena D and Devrnja, Nina and Kostić, Igor and Ćalić, Dušica",
year = "2012",
abstract = "Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.",
title = "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots",
pages = "396-389",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2887"
}

Zdravković-Korać, S., Tubić, L., Milojević, J. D., Devrnja, N., Kostić, I.,& Ćalić, D.. (2012). Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
Belgrade: Institute of Forestry., 389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887

Zdravković-Korać S, Tubić L, Milojević JD, Devrnja N, Kostić I, Ćalić D. Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.. 2012;:389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Milojević, Jelena D, Devrnja, Nina, Kostić, Igor, Ćalić, Dušica, "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots" in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. (2012):389-396,
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .