Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots
Аутори
Zdravković-Korać, SnežanaTubić, Ljiljana
Milojević, Jelena D
Devrnja, Nina
Kostić, Igor
Ćalić, Dušica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by c...utting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.
Кључне речи:
Aesculus hippocastanum; Rooting; Shoots-tip necrosisИзвор:
Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia., 2012, 389-396Издавач:
- Belgrade: Institute of Forestry
Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Zdravković-Korać, Snežana AU - Tubić, Ljiljana AU - Milojević, Jelena D AU - Devrnja, Nina AU - Kostić, Igor AU - Ćalić, Dušica PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2887 AB - Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed. PB - Belgrade: Institute of Forestry C3 - Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. T1 - Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots EP - 396 SP - 389 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2887 ER -
@conference{ author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Milojević, Jelena D and Devrnja, Nina and Kostić, Igor and Ćalić, Dušica", year = "2012", abstract = "Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.", publisher = "Belgrade: Institute of Forestry", journal = "Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.", title = "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots", pages = "396-389", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2887" }
Zdravković-Korać, S., Tubić, L., Milojević, J. D., Devrnja, N., Kostić, I.,& Ćalić, D.. (2012). Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. Belgrade: Institute of Forestry., 389-396. https://hdl.handle.net/21.15107/rcub_rimsi_2887
Zdravković-Korać S, Tubić L, Milojević JD, Devrnja N, Kostić I, Ćalić D. Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.. 2012;:389-396. https://hdl.handle.net/21.15107/rcub_rimsi_2887 .
Zdravković-Korać, Snežana, Tubić, Ljiljana, Milojević, Jelena D, Devrnja, Nina, Kostić, Igor, Ćalić, Dušica, "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots" in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. (2012):389-396, https://hdl.handle.net/21.15107/rcub_rimsi_2887 .