TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1699
AB  - Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY
SP  - 342
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1699
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY",
pages = "342",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1699"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):342,
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Đikanović, Daniela
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Jovanović, Jelena
AU  - Dragišić Maksimović, Jelena
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1704
AB  - Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE
SP  - 341
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1704
ER  - 

@conference{
author = "Bartolić, Dragana and Đikanović, Daniela and Stanković, Mira and Prokopijević, Miloš and Jovanović, Jelena and Dragišić Maksimović, Jelena and Radotić, Ksenija",
year = "2022",
abstract = "Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE",
pages = "341",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1704"
}

Bartolić, D., Đikanović, D., Stanković, M., Prokopijević, M., Jovanović, J., Dragišić Maksimović, J.,& Radotić, K.. (2022). INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704

Bartolić D, Đikanović D, Stanković M, Prokopijević M, Jovanović J, Dragišić Maksimović J, Radotić K. INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

Bartolić, Dragana, Đikanović, Daniela, Stanković, Mira, Prokopijević, Miloš, Jovanović, Jelena, Dragišić Maksimović, Jelena, Radotić, Ksenija, "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):341,
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1700
AB  - Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1700
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS",
pages = "203",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1700"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):203,
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1790
AB  - Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.
PB  - Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization
EP  - 342
SP  - 340
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1790
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Radotić, Ksenija and Prodanović, Radivoje",
year = "2021",
abstract = "Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.",
publisher = "Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization",
pages = "342-340",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1790"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Radotić, K.,& Prodanović, R.. (2021). Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 1, 340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Radotić K, Prodanović R. Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;1:340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Radotić, Ksenija, Prodanović, Radivoje, "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2021):340-342,
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

TY  - JOUR
AU  - Ilic-Durdic, Karla
AU  - Ostafe, Raluca
AU  - Prodanović, Olivera
AU  - Durdevic-Delmas, Aleksandra
AU  - Popović, Nikolina
AU  - Fischer, Rainer
AU  - Schillberg, Stefan
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1488
AB  - The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods. Lignin peroxidase (LiP) fromPhanerochaete chrysosporiumis a heme-containing lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules, including industrial dyes. This enzyme is therefore ideal as a starting point for protein engineering. Accordingly, we subjected two positions (165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis, and the resulting library of 10(4) independent clones was expressed on the surface of yeast cells. This yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more efficiently. We identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes (Evans blue, amido black 10B and Guinea green) and up to 13-fold higher catalytic activity. Additionally, cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis, achieving significant increases in both enzyme activity and stability compared to a whole-cell biocatalyst. LiP-coated cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 h. The best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.
PB  - Higher Education Press, Beijing
T2  - Frontiers of Environmental Science & Engineering
T1  - Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls
IS  - 2
VL  - 15
DO  - 10.1007/s11783-020-1311-4
ER  - 

@article{
author = "Ilic-Durdic, Karla and Ostafe, Raluca and Prodanović, Olivera and Durdevic-Delmas, Aleksandra and Popović, Nikolina and Fischer, Rainer and Schillberg, Stefan and Prodanović, Radivoje",
year = "2021",
abstract = "The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods. Lignin peroxidase (LiP) fromPhanerochaete chrysosporiumis a heme-containing lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules, including industrial dyes. This enzyme is therefore ideal as a starting point for protein engineering. Accordingly, we subjected two positions (165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis, and the resulting library of 10(4) independent clones was expressed on the surface of yeast cells. This yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more efficiently. We identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes (Evans blue, amido black 10B and Guinea green) and up to 13-fold higher catalytic activity. Additionally, cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis, achieving significant increases in both enzyme activity and stability compared to a whole-cell biocatalyst. LiP-coated cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 h. The best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.",
publisher = "Higher Education Press, Beijing",
journal = "Frontiers of Environmental Science & Engineering",
title = "Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls",
number = "2",
volume = "15",
doi = "10.1007/s11783-020-1311-4"
}

Ilic-Durdic, K., Ostafe, R., Prodanović, O., Durdevic-Delmas, A., Popović, N., Fischer, R., Schillberg, S.,& Prodanović, R.. (2021). Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls. in Frontiers of Environmental Science & Engineering
Higher Education Press, Beijing., 15(2).
https://doi.org/10.1007/s11783-020-1311-4

Ilic-Durdic K, Ostafe R, Prodanović O, Durdevic-Delmas A, Popović N, Fischer R, Schillberg S, Prodanović R. Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls. in Frontiers of Environmental Science & Engineering. 2021;15(2).
doi:10.1007/s11783-020-1311-4 .

Ilic-Durdic, Karla, Ostafe, Raluca, Prodanović, Olivera, Durdevic-Delmas, Aleksandra, Popović, Nikolina, Fischer, Rainer, Schillberg, Stefan, Prodanović, Radivoje, "Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls" in Frontiers of Environmental Science & Engineering, 15, no. 2 (2021),
https://doi.org/10.1007/s11783-020-1311-4 . .

TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
AB  - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
EP  - 3328
IS  - 3
SP  - 3320
VL  - 27
DO  - 10.1007/s11356-019-07200-9
ER  - 

@article{
author = "Zakrzewska, Joanna and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija",
year = "2020",
abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence",
pages = "3328-3320",
number = "3",
volume = "27",
doi = "10.1007/s11356-019-07200-9"
}

Zakrzewska, J., Mitrović, A. Lj., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(3), 3320-3328.
https://doi.org/10.1007/s11356-019-07200-9

Zakrzewska J, Mitrović AL, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328.
doi:10.1007/s11356-019-07200-9 .

Zakrzewska, Joanna, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328,
https://doi.org/10.1007/s11356-019-07200-9 . .

TY  - JOUR
AU  - Cvetić-Antić, Tijana
AU  - Janosević, Dusica
AU  - Maksimović, Vuk
AU  - Zivić, Miroslav
AU  - Budimir, Snežana M
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
ER  - 

@article{
author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086"
}

Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 244.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244.
doi:10.1016/j.jplph.2019.153086 .

Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020),
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
AU  - Stanković, Mira
AU  - Krstović, Sasa
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1358
AB  - BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship
EP  - 2910
IS  - 7
SP  - 2905
VL  - 100
DO  - 10.1002/jsfa.10317
ER  - 

@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Dragišić Maksimović, Jelena and Stanković, Mira and Krstović, Sasa and Baosic, Rada and Radotić, Ksenija",
year = "2020",
abstract = "BACKGROUND Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose-response manner, with a ratio of below 1 up to a concentration of 51.51 mu g kg(-1), and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 mu g kg(-1), respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose-response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship",
pages = "2910-2905",
number = "7",
volume = "100",
doi = "10.1002/jsfa.10317"
}

Bartolić, D., Maksimović, V., Dragišić Maksimović, J., Stanković, M., Krstović, S., Baosic, R.,& Radotić, K.. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317

Bartolić D, Maksimović V, Dragišić Maksimović J, Stanković M, Krstović S, Baosic R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship. in Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910.
doi:10.1002/jsfa.10317 .

Bartolić, Dragana, Maksimović, Vuk, Dragišić Maksimović, Jelena, Stanković, Mira, Krstović, Sasa, Baosic, Rada, Radotić, Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose-response relationship" in Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Nikčević, Miroslav
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1319
AB  - Proteins and phenols are minor, but important components of honey. Phenols originate from nectar and proteins from nectar/pollen and bees themselves. These two types of compounds are important for growth and development of bee colonies, and also for their defense capacity. Their quantity in honey depends on food accessibility and forage during the year. In this preliminary study, we aimed to see yearly dynamics of ratio of phenols and proteins in honey for a bee colony, and to observe the possible effects of extended oxalic acid antivarroa treatment on honey composition regarding proteins and phenols. Since proteins and phenols are fluorescently active, for measurement of phenol/protein ratio in honey we applied steady-state fluorescence spectroscopy as a rapid and non-destructive method. We determined the ratio of the spectral components related to these two main fluorophores in honey, proteins and phenolic compounds, as a ratiometric indicator of variability in honey samples collected in different seasons. We found that oxalic acid does not influence proteins' and phenolics' composition of honey, based on the spectral analysis. Since proteins in honey originate mainly from bees colony, this result indicates that oxalic acid did not change bee synthesis of proteins, indicating that oxalic acid, as a protective agent, is not harmful to the bees.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition
EP  - 1518
IS  - 7
SP  - 1515
VL  - 246
DO  - 10.1007/s00217-020-03507-x
ER  - 

@article{
author = "Stanković, Mira and Nikčević, Miroslav and Radotić, Ksenija",
year = "2020",
abstract = "Proteins and phenols are minor, but important components of honey. Phenols originate from nectar and proteins from nectar/pollen and bees themselves. These two types of compounds are important for growth and development of bee colonies, and also for their defense capacity. Their quantity in honey depends on food accessibility and forage during the year. In this preliminary study, we aimed to see yearly dynamics of ratio of phenols and proteins in honey for a bee colony, and to observe the possible effects of extended oxalic acid antivarroa treatment on honey composition regarding proteins and phenols. Since proteins and phenols are fluorescently active, for measurement of phenol/protein ratio in honey we applied steady-state fluorescence spectroscopy as a rapid and non-destructive method. We determined the ratio of the spectral components related to these two main fluorophores in honey, proteins and phenolic compounds, as a ratiometric indicator of variability in honey samples collected in different seasons. We found that oxalic acid does not influence proteins' and phenolics' composition of honey, based on the spectral analysis. Since proteins in honey originate mainly from bees colony, this result indicates that oxalic acid did not change bee synthesis of proteins, indicating that oxalic acid, as a protective agent, is not harmful to the bees.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition",
pages = "1518-1515",
number = "7",
volume = "246",
doi = "10.1007/s00217-020-03507-x"
}

Stanković, M., Nikčević, M.,& Radotić, K.. (2020). Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition. in European Food Research and Technology
Springer, New York., 246(7), 1515-1518.
https://doi.org/10.1007/s00217-020-03507-x

Stanković M, Nikčević M, Radotić K. Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition. in European Food Research and Technology. 2020;246(7):1515-1518.
doi:10.1007/s00217-020-03507-x .

Stanković, Mira, Nikčević, Miroslav, Radotić, Ksenija, "Annual variation of proteins and phenols in honey of a bee society using fluorescence spectroscopy: a way to assess effects of antivarroa treatments on honey composition" in European Food Research and Technology, 246, no. 7 (2020):1515-1518,
https://doi.org/10.1007/s00217-020-03507-x . .

TY  - JOUR
AU  - Balaž, Ana Marija
AU  - BLazic, Marija B.
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Ostafe, Raluca
AU  - Fischer, Rainer
AU  - Prodanović, Radivoje
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1297
AB  - Production of soluble cellobiose dehydrogenase (CDH) mutant proteins previously evolved on the surface of S. cerevisiae yeast cells was established for use in biosensors and biofuel cells. For this purpose, mutant cdh genes tm (D20N, A64T, V592M), H5 (D20N, V22A, A64T, V592M) and H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S) were cloned to pPICZ alpha plasmid and transformed into Pichia pastoris KM71H strain for high expression in a soluble form and kinetic characterization. After 6 days of expression under methanol induction, the CDHs were purified by ultrafiltration, ion-exchange chromatography and gel filtration. Sodium dodecyl sulfate electrophoresis confirmed the purity and presence of a single protein band at a molecular weight of 100 kDa. Kinetic characterization showed that the H5 mutant had the highest catalytic constant of 43.5 s(-1) for lactose, while the mutant H9 showed the highest specificity constant for lactose of 132 mM(-1) s(-1). All three mutant proteins did not change the pH optimum that was between 4.5 and 5.5. Compared to the previously obtained wild types and mutants of CDH from Phanerochaete chrysosporium, the variants reported in this article had higher activity and specificity that together with high protein expression rate in P. pastoris, makes them good candidates for use in biotechnology for lactobionic acid production and biosensor manufacture.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain
EP  - 35
IS  - 1
SP  - 25
VL  - 85
DO  - 10.2298/JSC190320058B
ER  - 

@article{
author = "Balaž, Ana Marija and BLazic, Marija B. and Popović, Nikolina and Prodanović, Olivera and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje",
year = "2020",
abstract = "Production of soluble cellobiose dehydrogenase (CDH) mutant proteins previously evolved on the surface of S. cerevisiae yeast cells was established for use in biosensors and biofuel cells. For this purpose, mutant cdh genes tm (D20N, A64T, V592M), H5 (D20N, V22A, A64T, V592M) and H9 (D20N, A64T, T84A, A261P, V592M, E674G, N715S) were cloned to pPICZ alpha plasmid and transformed into Pichia pastoris KM71H strain for high expression in a soluble form and kinetic characterization. After 6 days of expression under methanol induction, the CDHs were purified by ultrafiltration, ion-exchange chromatography and gel filtration. Sodium dodecyl sulfate electrophoresis confirmed the purity and presence of a single protein band at a molecular weight of 100 kDa. Kinetic characterization showed that the H5 mutant had the highest catalytic constant of 43.5 s(-1) for lactose, while the mutant H9 showed the highest specificity constant for lactose of 132 mM(-1) s(-1). All three mutant proteins did not change the pH optimum that was between 4.5 and 5.5. Compared to the previously obtained wild types and mutants of CDH from Phanerochaete chrysosporium, the variants reported in this article had higher activity and specificity that together with high protein expression rate in P. pastoris, makes them good candidates for use in biotechnology for lactobionic acid production and biosensor manufacture.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain",
pages = "35-25",
number = "1",
volume = "85",
doi = "10.2298/JSC190320058B"
}

Balaž, A. M., BLazic, M. B., Popović, N., Prodanović, O., Ostafe, R., Fischer, R.,& Prodanović, R.. (2020). Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(1), 25-35.
https://doi.org/10.2298/JSC190320058B

Balaž AM, BLazic MB, Popović N, Prodanović O, Ostafe R, Fischer R, Prodanović R. Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain. in Journal of the Serbian Chemical Society. 2020;85(1):25-35.
doi:10.2298/JSC190320058B .

Balaž, Ana Marija, BLazic, Marija B., Popović, Nikolina, Prodanović, Olivera, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain" in Journal of the Serbian Chemical Society, 85, no. 1 (2020):25-35,
https://doi.org/10.2298/JSC190320058B . .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana M.
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Savić, Danijela Z
AU  - Grguric-Sipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1344
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O-2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors
EP  - 127
SP  - 123
VL  - 148
DO  - 10.1016/j.freeradbiomed.2020.01.001
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana M. and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena and Savić, Danijela Z and Grguric-Sipka, Sanja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O-2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors",
pages = "127-123",
volume = "148",
doi = "10.1016/j.freeradbiomed.2020.01.001"
}

Korać Jačić, J., Nikolić, L. M., Stanković, D. M., Opačić, M., Dimitrijević, M., Savić, D. Z., Grguric-Sipka, S., Spasojević, I.,& Bogdanović Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić LM, Stanković DM, Opačić M, Dimitrijević M, Savić DZ, Grguric-Sipka S, Spasojević I, Bogdanović Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana M., Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena, Savić, Danijela Z, Grguric-Sipka, Sanja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors" in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - JOUR
AU  - Milosavljević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Milivojević, Jasminka M
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1359
AB  - Phenolic compounds of 12 strawberry cultivars were profiled using spectrophotometry, spectrofluorometry, and high-performance liquid chromatography-mass spectrometry, coupled with multivariate regression analysis. Total phenolic content, total anthocyanin content, and total antioxidant capacity (TPC, TACY, and TAC, respectively) and concentrations of individual phenolics were evaluated, and the multivariate statistic was employed to identify the most promising cultivars based on their phenolic content. According to the principal component analysis, TAC was strongly correlated with the TPC (0.81), pointing out its importance in overall antioxidant activity. 'Joly', 'Laetitia', and 'Asia' cultivars were distinguished from others as the richest in concerning identified anthocyanins, almost all flavonoids and phenolic acids. Multivariate curve resolution with alternating least squares indicated the presence of two main types of fluorophores assigned to anthocyanins and phenolics, in which emission spectral ratios also showed the highest values in the referred cultivars. These cultivars could be recommended to consumers in terms of the health functionality of fruit.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods
EP  - 4354
IS  - 15
SP  - 4346
VL  - 68
DO  - 10.1021/acs.jafc.9b07331
ER  - 

@article{
author = "Milosavljević, Dragica and Mutavdžić, Dragosav and Radotić, Ksenija and Milivojević, Jasminka M and Maksimović, Vuk and Dragišić Maksimović, Jelena",
year = "2020",
abstract = "Phenolic compounds of 12 strawberry cultivars were profiled using spectrophotometry, spectrofluorometry, and high-performance liquid chromatography-mass spectrometry, coupled with multivariate regression analysis. Total phenolic content, total anthocyanin content, and total antioxidant capacity (TPC, TACY, and TAC, respectively) and concentrations of individual phenolics were evaluated, and the multivariate statistic was employed to identify the most promising cultivars based on their phenolic content. According to the principal component analysis, TAC was strongly correlated with the TPC (0.81), pointing out its importance in overall antioxidant activity. 'Joly', 'Laetitia', and 'Asia' cultivars were distinguished from others as the richest in concerning identified anthocyanins, almost all flavonoids and phenolic acids. Multivariate curve resolution with alternating least squares indicated the presence of two main types of fluorophores assigned to anthocyanins and phenolics, in which emission spectral ratios also showed the highest values in the referred cultivars. These cultivars could be recommended to consumers in terms of the health functionality of fruit.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods",
pages = "4354-4346",
number = "15",
volume = "68",
doi = "10.1021/acs.jafc.9b07331"
}

Milosavljević, D., Mutavdžić, D., Radotić, K., Milivojević, J. M., Maksimović, V.,& Dragišić Maksimović, J.. (2020). Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 68(15), 4346-4354.
https://doi.org/10.1021/acs.jafc.9b07331

Milosavljević D, Mutavdžić D, Radotić K, Milivojević JM, Maksimović V, Dragišić Maksimović J. Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods. in Journal of Agricultural and Food Chemistry. 2020;68(15):4346-4354.
doi:10.1021/acs.jafc.9b07331 .

Milosavljević, Dragica, Mutavdžić, Dragosav, Radotić, Ksenija, Milivojević, Jasminka M, Maksimović, Vuk, Dragišić Maksimović, Jelena, "Phenolic Profiling of 12 Strawberry Cultivars Using Different Spectroscopic Methods" in Journal of Agricultural and Food Chemistry, 68, no. 15 (2020):4346-4354,
https://doi.org/10.1021/acs.jafc.9b07331 . .

TY  - JOUR
AU  - Willaert, Ronnie
AU  - Vanden, Boer, Pieterjan
AU  - Malovichko, Anton
AU  - Alioscha-Perez, Mitchel
AU  - Radotić, Ksenija
AU  - Bartolić, Dragana
AU  - Kalauzi, Aleksandar
AU  - Villalba, Maria Ines
AU  - Sanglard, Dominique
AU  - Dietler, Giovanni
AU  - Sahli, Hichem
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1335
AB  - Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
PB  - Amer Assoc Advancement Science, Washington
T2  - Science Advances
T1  - Single yeast cell nanomotions correlate with cellular activity
IS  - 26
VL  - 6
DO  - 10.1126/sciadv.aba3139
ER  - 

@article{
author = "Willaert, Ronnie and Vanden, Boer, Pieterjan and Malovichko, Anton and Alioscha-Perez, Mitchel and Radotić, Ksenija and Bartolić, Dragana and Kalauzi, Aleksandar and Villalba, Maria Ines and Sanglard, Dominique and Dietler, Giovanni and Sahli, Hichem and Kasas, Sandor",
year = "2020",
abstract = "Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.",
publisher = "Amer Assoc Advancement Science, Washington",
journal = "Science Advances",
title = "Single yeast cell nanomotions correlate with cellular activity",
number = "26",
volume = "6",
doi = "10.1126/sciadv.aba3139"
}

Willaert, R., Vanden, B. P., Malovichko, A., Alioscha-Perez, M., Radotić, K., Bartolić, D., Kalauzi, A., Villalba, M. I., Sanglard, D., Dietler, G., Sahli, H.,& Kasas, S.. (2020). Single yeast cell nanomotions correlate with cellular activity. in Science Advances
Amer Assoc Advancement Science, Washington., 6(26).
https://doi.org/10.1126/sciadv.aba3139

Willaert R, Vanden BP, Malovichko A, Alioscha-Perez M, Radotić K, Bartolić D, Kalauzi A, Villalba MI, Sanglard D, Dietler G, Sahli H, Kasas S. Single yeast cell nanomotions correlate with cellular activity. in Science Advances. 2020;6(26).
doi:10.1126/sciadv.aba3139 .

Willaert, Ronnie, Vanden, Boer, Pieterjan, Malovichko, Anton, Alioscha-Perez, Mitchel, Radotić, Ksenija, Bartolić, Dragana, Kalauzi, Aleksandar, Villalba, Maria Ines, Sanglard, Dominique, Dietler, Giovanni, Sahli, Hichem, Kasas, Sandor, "Single yeast cell nanomotions correlate with cellular activity" in Science Advances, 6, no. 26 (2020),
https://doi.org/10.1126/sciadv.aba3139 . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Jovic-Jovicic, Nataša
AU  - Mojović, Zorica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1342
AB  - Cellulose, xylan and lignin, as well as maize cell wall, were used as modifiers of carbon paste electrode. Comparative analysis of electrochemical performance of each modifier toward heavy metal detection was performed. A higher affinity of biomass constituents for lead ions than for copper ions influenced simultaneous detection of lead and copper ions. The new procedure for the simultaneous detection of lead and copper ions based on their co-adsorption was presented.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Electroanalytical Chemistry
T1  - Comparative study of lignocellulosic biomass and its components as electrode modifiers for detection of lead and copper ions
VL  - 862
DO  - 10.1016/j.jelechem.2020.114010
ER  - 

@article{
author = "Radotić, Ksenija and Đikanović, Daniela and Simonović Radosavljević, Jasna and Jovic-Jovicic, Nataša and Mojović, Zorica",
year = "2020",
abstract = "Cellulose, xylan and lignin, as well as maize cell wall, were used as modifiers of carbon paste electrode. Comparative analysis of electrochemical performance of each modifier toward heavy metal detection was performed. A higher affinity of biomass constituents for lead ions than for copper ions influenced simultaneous detection of lead and copper ions. The new procedure for the simultaneous detection of lead and copper ions based on their co-adsorption was presented.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Electroanalytical Chemistry",
title = "Comparative study of lignocellulosic biomass and its components as electrode modifiers for detection of lead and copper ions",
volume = "862",
doi = "10.1016/j.jelechem.2020.114010"
}

Radotić, K., Đikanović, D., Simonović Radosavljević, J., Jovic-Jovicic, N.,& Mojović, Z.. (2020). Comparative study of lignocellulosic biomass and its components as electrode modifiers for detection of lead and copper ions. in Journal of Electroanalytical Chemistry
Elsevier Science Sa, Lausanne., 862.
https://doi.org/10.1016/j.jelechem.2020.114010

Radotić K, Đikanović D, Simonović Radosavljević J, Jovic-Jovicic N, Mojović Z. Comparative study of lignocellulosic biomass and its components as electrode modifiers for detection of lead and copper ions. in Journal of Electroanalytical Chemistry. 2020;862.
doi:10.1016/j.jelechem.2020.114010 .

Radotić, Ksenija, Đikanović, Daniela, Simonović Radosavljević, Jasna, Jovic-Jovicic, Nataša, Mojović, Zorica, "Comparative study of lignocellulosic biomass and its components as electrode modifiers for detection of lead and copper ions" in Journal of Electroanalytical Chemistry, 862 (2020),
https://doi.org/10.1016/j.jelechem.2020.114010 . .

TY  - JOUR
AU  - Milojkov, Dusan V.
AU  - Silvestre, Oscar F.
AU  - Stanic, Vojislav
AU  - Janjic, Goran V.
AU  - Mutavdžić, Dragosav
AU  - Milanović, Marija
AU  - Nieder, Jana B.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1387
AB  - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiples states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (P-3(0)-> H-3(5) transition of Pr3+ ion) and orange emission at 600 nm (D-1(2)-> H-3(4)), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.
PB  - Amsterdam : Elsevier
T2  - Journal of Luminescence
T1  - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
VL  - 217
DO  - 10.1016/j.jlumin.2019.116757
ER  - 

@article{
author = "Milojkov, Dusan V. and Silvestre, Oscar F. and Stanic, Vojislav and Janjic, Goran V. and Mutavdžić, Dragosav and Milanović, Marija and Nieder, Jana B.",
year = "2020",
abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiples states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (P-3(0)-> H-3(5) transition of Pr3+ ion) and orange emission at 600 nm (D-1(2)-> H-3(4)), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.",
publisher = "Amsterdam : Elsevier",
journal = "Journal of Luminescence",
title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents",
volume = "217",
doi = "10.1016/j.jlumin.2019.116757"
}

Milojkov, D. V., Silvestre, O. F., Stanic, V., Janjic, G. V., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence
Amsterdam : Elsevier., 217.
https://doi.org/10.1016/j.jlumin.2019.116757

Milojkov DV, Silvestre OF, Stanic V, Janjic GV, Mutavdžić D, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217.
doi:10.1016/j.jlumin.2019.116757 .

Milojkov, Dusan V., Silvestre, Oscar F., Stanic, Vojislav, Janjic, Goran V., Mutavdžić, Dragosav, Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020),
https://doi.org/10.1016/j.jlumin.2019.116757 . .

TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Celine
AU  - Stupar, Petar
AU  - Villalba, Maria, I
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pesic, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1338
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
PB  - Wiley, Hoboken
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
IS  - 12
VL  - 33
DO  - 10.1002/jmr.2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Celine and Stupar, Petar and Villalba, Maria, I and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pesic, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
publisher = "Wiley, Hoboken",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
number = "12",
volume = "33",
doi = "10.1002/jmr.2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pesic, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition
Wiley, Hoboken., 33(12).
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pesic M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;33(12).
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Celine, Stupar, Petar, Villalba, Maria, I, Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pesic, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition, 33, no. 12 (2020),
https://doi.org/10.1002/jmr.2849 . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melo, Thor Bernt
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1298
AB  - For investigations of ongoing processes in plants, such as photosynthesis in conifer leaves, nondestructive and noninvasive measuring techniques are needed. In this paper, a novel approach has been developed for the measurement of chloroplasts' numbers and pigment contents in conifer leaves based on the measurements of leaf absorption spectra using optical fibers and an array spectrophotometer. To eliminate the effect of scattering on the measured absorption spectra, a strategy has been applied taking advantage of the combined use of thin optical fibers normal to the needle's longitudinal axis and the phenomenon that scattering is largest in the forward direction. The optical path in the leaf is nearly the distance between the fiber tips; thus, we were able to obtain the absorption spectrum of the pigments in situ. A effect of the measured absorption spectra, occurring due to the organization of pigments in the leaf and interaction between light and leaf interior, can be accounted for by using the so-called Duysens transformation. Using this transformation, pigment contents and the relative number of chloroplasts can be obtained from the measured absorption spectra. We applied the method to observe pigment concentrations in different stages of the greening process in the leaves of two conifer species, Taxus baccata and Picea abies. The presented method may be used to estimate changes in chloroplast number and pigment content during various phases of greening of a species and to observe differences among various species.
PB  - Springer, Dordrecht
T2  - Journal of Biological Physics
T1  - Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content
EP  - 43
IS  - 1
SP  - 33
VL  - 46
DO  - 10.1007/s10867-020-09539-4
ER  - 

@article{
author = "Radotić, Ksenija and Melo, Thor Bernt",
year = "2020",
abstract = "For investigations of ongoing processes in plants, such as photosynthesis in conifer leaves, nondestructive and noninvasive measuring techniques are needed. In this paper, a novel approach has been developed for the measurement of chloroplasts' numbers and pigment contents in conifer leaves based on the measurements of leaf absorption spectra using optical fibers and an array spectrophotometer. To eliminate the effect of scattering on the measured absorption spectra, a strategy has been applied taking advantage of the combined use of thin optical fibers normal to the needle's longitudinal axis and the phenomenon that scattering is largest in the forward direction. The optical path in the leaf is nearly the distance between the fiber tips; thus, we were able to obtain the absorption spectrum of the pigments in situ. A effect of the measured absorption spectra, occurring due to the organization of pigments in the leaf and interaction between light and leaf interior, can be accounted for by using the so-called Duysens transformation. Using this transformation, pigment contents and the relative number of chloroplasts can be obtained from the measured absorption spectra. We applied the method to observe pigment concentrations in different stages of the greening process in the leaves of two conifer species, Taxus baccata and Picea abies. The presented method may be used to estimate changes in chloroplast number and pigment content during various phases of greening of a species and to observe differences among various species.",
publisher = "Springer, Dordrecht",
journal = "Journal of Biological Physics",
title = "Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content",
pages = "43-33",
number = "1",
volume = "46",
doi = "10.1007/s10867-020-09539-4"
}

Radotić, K.,& Melo, T. B.. (2020). Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content. in Journal of Biological Physics
Springer, Dordrecht., 46(1), 33-43.
https://doi.org/10.1007/s10867-020-09539-4

Radotić K, Melo TB. Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content. in Journal of Biological Physics. 2020;46(1):33-43.
doi:10.1007/s10867-020-09539-4 .

Radotić, Ksenija, Melo, Thor Bernt, "Using optical fibers to measure absorption in intact conifer leaves, relative numbers of chloroplasts, and pigment content" in Journal of Biological Physics, 46, no. 1 (2020):33-43,
https://doi.org/10.1007/s10867-020-09539-4 . .

TY  - CONF
AU  - Pantić, Nevena
AU  - Popović, Nikolina
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Radivoje
AU  - Đikanović, Daniela
AU  - Prodanović, Olivera
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1656
AB  - Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL
SP  - 220-223
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1656
ER  - 

@conference{
author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Đikanović, Daniela and Prodanović, Olivera",
year = "2019",
abstract = "Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL",
pages = "220-223",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1656"
}

Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Đikanović, D.,& Prodanović, O.. (2019). OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 220-223.
https://hdl.handle.net/21.15107/rcub_rimsi_1656

Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Đikanović D, Prodanović O. OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research. 2019;:220-223.
https://hdl.handle.net/21.15107/rcub_rimsi_1656 .

Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Đikanović, Daniela, Prodanović, Olivera, "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL" in 27th International Conference Ecological Truth and Environmental Research (2019):220-223,
https://hdl.handle.net/21.15107/rcub_rimsi_1656 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Simonović Radosavljević, Jasna
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1794
AB  - Maize seeds are a primary source of nourishment and their viability is a critical consideration to
ensure a reasonably high harvest and seed quality. In this work, we study the effect of aflatoxin
induced stress on the seed viability concerning the germination, as well as the estimation on the
impact on the viability using fluorescence spectroscopy. Our results showed that the contaminated
seeds exhibit significant decreases in the percent of germination, even at low levels, if compared to the
uncontaminated seeds (P < 0.05). Furthermore, the fluorescence ratio of 453/680 and 680/751, could
be used for the rapid screening of the viability of the maize seeds.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy
EP  - 304
SP  - 301
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1794
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Simonović Radosavljević, Jasna and Radotić, Ksenija",
year = "2019",
abstract = "Maize seeds are a primary source of nourishment and their viability is a critical consideration to
ensure a reasonably high harvest and seed quality. In this work, we study the effect of aflatoxin
induced stress on the seed viability concerning the germination, as well as the estimation on the
impact on the viability using fluorescence spectroscopy. Our results showed that the contaminated
seeds exhibit significant decreases in the percent of germination, even at low levels, if compared to the
uncontaminated seeds (P < 0.05). Furthermore, the fluorescence ratio of 453/680 and 680/751, could
be used for the rapid screening of the viability of the maize seeds.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy",
pages = "304-301",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1794"
}

Bartolić, D., Stanković, M., Mitrović, A. Lj., Mutavdžić, D., Simonović Radosavljević, J.,& Radotić, K.. (2019). Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 301-304.
https://hdl.handle.net/21.15107/rcub_rimsi_1794

Bartolić D, Stanković M, Mitrović AL, Mutavdžić D, Simonović Radosavljević J, Radotić K. Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy. in 27th International Conference Ecological Truth and Environmental Research. 2019;:301-304.
https://hdl.handle.net/21.15107/rcub_rimsi_1794 .

Bartolić, Dragana, Stanković, Mira, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Simonović Radosavljević, Jasna, Radotić, Ksenija, "Viability assessment of maize (Zea mays L.) seeds contaminated with aflatoxin using fluorescence spectroscopy" in 27th International Conference Ecological Truth and Environmental Research (2019):301-304,
https://hdl.handle.net/21.15107/rcub_rimsi_1794 .

TY  - CONF
AU  - Stanković, Mira
AU  - Simonović Radosavljević, Jasna
AU  - Đikanović, Daniela
AU  - Spasojević, Dragica
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1807
AB  - In this pilot research, we showed that pollen autofluorescence varied between the pollen of the
different botanical species. Our findings suggest that classification of pollen grains may be obtained
based on their fluorescence images.
PB  - University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia
PB  - Serbian Society for Microscopy, Serbia
C3  - 14th MULTINATIONAL CONGRESS ON MICROSCOPY
T1  - The use of fluorescence microscopy for classification of pollen grains
SP  - 171-173
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1807
ER  - 

@conference{
author = "Stanković, Mira and Simonović Radosavljević, Jasna and Đikanović, Daniela and Spasojević, Dragica and Radotić, Ksenija",
year = "2019",
abstract = "In this pilot research, we showed that pollen autofluorescence varied between the pollen of the
different botanical species. Our findings suggest that classification of pollen grains may be obtained
based on their fluorescence images.",
publisher = "University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Serbian Society for Microscopy, Serbia",
journal = "14th MULTINATIONAL CONGRESS ON MICROSCOPY",
title = "The use of fluorescence microscopy for classification of pollen grains",
pages = "171-173",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1807"
}

Stanković, M., Simonović Radosavljević, J., Đikanović, D., Spasojević, D.,& Radotić, K.. (2019). The use of fluorescence microscopy for classification of pollen grains. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia., 171-173.
https://hdl.handle.net/21.15107/rcub_rimsi_1807

Stanković M, Simonović Radosavljević J, Đikanović D, Spasojević D, Radotić K. The use of fluorescence microscopy for classification of pollen grains. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY. 2019;:171-173.
https://hdl.handle.net/21.15107/rcub_rimsi_1807 .

Stanković, Mira, Simonović Radosavljević, Jasna, Đikanović, Daniela, Spasojević, Dragica, Radotić, Ksenija, "The use of fluorescence microscopy for classification of pollen grains" in 14th MULTINATIONAL CONGRESS ON MICROSCOPY (2019):171-173,
https://hdl.handle.net/21.15107/rcub_rimsi_1807 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Pantić, Nevena
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Simonović Radosavljević, Jasna
AU  - Đikanović, Daniela
AU  - Prodanović, Radivoje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1657
AB  - Horseradish peroxidase (HRP, E.C. 1.11.1.7) catalyzes oxidation of aqueous aromatic compounds
using hydrogen peroxide. Enzymatic treatment methods for phenol removal from wastewaters has
become an efficient and environmentally friendly alternative for the traditional methods.
Carboxymethyl cellulose (CMC) derivative with tyramine attached via amide bond to carboxyl groups
has been chosen as carrier for immobilization. In effort to overcome the main disadvantage of
entrapment immobilization method, loss of enzyme activity due to washing out from the carrier, HRP
was modified in a reductive amination reaction and tyramine was bound to the enzyme.
Immobilization of tyramine-HRP onto tyramide-carboxymethyl cellulose carrier was carried in an
emulsion polymerization reaction that produced carboxymethyl cellulose microbeads. The highest
specific activity of the obtained biocatalyst was 0.227 U/ml and after 48h of storage 0.197 U/ml.
Immobilized tyramine-HRP retained 87% of activity after 48 h. Immobilized HRP is a suitable
candidate for wastewater treatment.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT
SP  - 224-227
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1657
ER  - 

@conference{
author = "Prokopijević, Miloš and Pantić, Nevena and Spasojević, Dragica and Prodanović, Olivera and Simonović Radosavljević, Jasna and Đikanović, Daniela and Prodanović, Radivoje",
year = "2019",
abstract = "Horseradish peroxidase (HRP, E.C. 1.11.1.7) catalyzes oxidation of aqueous aromatic compounds
using hydrogen peroxide. Enzymatic treatment methods for phenol removal from wastewaters has
become an efficient and environmentally friendly alternative for the traditional methods.
Carboxymethyl cellulose (CMC) derivative with tyramine attached via amide bond to carboxyl groups
has been chosen as carrier for immobilization. In effort to overcome the main disadvantage of
entrapment immobilization method, loss of enzyme activity due to washing out from the carrier, HRP
was modified in a reductive amination reaction and tyramine was bound to the enzyme.
Immobilization of tyramine-HRP onto tyramide-carboxymethyl cellulose carrier was carried in an
emulsion polymerization reaction that produced carboxymethyl cellulose microbeads. The highest
specific activity of the obtained biocatalyst was 0.227 U/ml and after 48h of storage 0.197 U/ml.
Immobilized tyramine-HRP retained 87% of activity after 48 h. Immobilized HRP is a suitable
candidate for wastewater treatment.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT",
pages = "224-227",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1657"
}

Prokopijević, M., Pantić, N., Spasojević, D., Prodanović, O., Simonović Radosavljević, J., Đikanović, D.,& Prodanović, R.. (2019). IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 224-227.
https://hdl.handle.net/21.15107/rcub_rimsi_1657

Prokopijević M, Pantić N, Spasojević D, Prodanović O, Simonović Radosavljević J, Đikanović D, Prodanović R. IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT. in 27th International Conference Ecological Truth and Environmental Research. 2019;:224-227.
https://hdl.handle.net/21.15107/rcub_rimsi_1657 .

Prokopijević, Miloš, Pantić, Nevena, Spasojević, Dragica, Prodanović, Olivera, Simonović Radosavljević, Jasna, Đikanović, Daniela, Prodanović, Radivoje, "IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT" in 27th International Conference Ecological Truth and Environmental Research (2019):224-227,
https://hdl.handle.net/21.15107/rcub_rimsi_1657 .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Stevanić, Jasna
AU  - Đikanović, Daniela
AU  - Mitrović, Aleksandra Lj.
AU  - Salmén, Lennart
AU  - Radotić, Ksenija
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1808
AB  - It has been shown that xylan is oriented in parallel to the cellulose and more or less parallel to the
axis of a cell wall, in isolated CW fragments from maize leaves. There was also a clear indication of
lignin orientation parallel to the longitudinal CW axis. This means that all of these components show
strong anisotropic behaviour and organisation.
PB  - University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia
PB  - Serbian Society for Microscopy, Serbia
C3  - 14th MULTINATIONAL CONGRESS ON MICROSCOPY
T1  - Imaging FTIR microscopy – technique for rapid screening of plant cell walls
SP  - 159-161
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1808
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Stevanić, Jasna and Đikanović, Daniela and Mitrović, Aleksandra Lj. and Salmén, Lennart and Radotić, Ksenija and Radotić, Ksenija",
year = "2019",
abstract = "It has been shown that xylan is oriented in parallel to the cellulose and more or less parallel to the
axis of a cell wall, in isolated CW fragments from maize leaves. There was also a clear indication of
lignin orientation parallel to the longitudinal CW axis. This means that all of these components show
strong anisotropic behaviour and organisation.",
publisher = "University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Serbian Society for Microscopy, Serbia",
journal = "14th MULTINATIONAL CONGRESS ON MICROSCOPY",
title = "Imaging FTIR microscopy – technique for rapid screening of plant cell walls",
pages = "159-161",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1808"
}

Simonović Radosavljević, J., Stevanić, J., Đikanović, D., Mitrović, A. Lj., Salmén, L., Radotić, K.,& Radotić, K.. (2019). Imaging FTIR microscopy – technique for rapid screening of plant cell walls. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia., 159-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1808

Simonović Radosavljević J, Stevanić J, Đikanović D, Mitrović AL, Salmén L, Radotić K, Radotić K. Imaging FTIR microscopy – technique for rapid screening of plant cell walls. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY. 2019;:159-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1808 .

Simonović Radosavljević, Jasna, Stevanić, Jasna, Đikanović, Daniela, Mitrović, Aleksandra Lj., Salmén, Lennart, Radotić, Ksenija, Radotić, Ksenija, "Imaging FTIR microscopy – technique for rapid screening of plant cell walls" in 14th MULTINATIONAL CONGRESS ON MICROSCOPY (2019):159-161,
https://hdl.handle.net/21.15107/rcub_rimsi_1808 .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Pantić, Nevena
AU  - Stevanić, Jasna
AU  - Đikanović, Daniela
AU  - Mitrović, Aleksandra Lj.
AU  - Salmén, Lennart
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1811
AB  - Cell wall can be considered as a nano-composite in which cellulose, lignin and hemicelluloses are
interconnected in a specific manner. Mechanical and physical propreties of plant fibres are dependent
on the orientation of constituent polymers (cellulose, hemicellulose, lignin). Fourier transform
infrared (FTIR) microscopy was used to examine the orientation of the main plant polymers in
transversal and longitudinal direction of the isolated cell wall of the maize leaves. Polarised FTIR
measurements indicated an anisotropy, i.e. orientation of the cellulose microfibrils that was more or
less parallel to the longitudinal axis of the cell wall. Xylan has parallel orientation with regard to the
orientation of cellulose, as well as lignin.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth & Environmental Research
T1  - STRUCTURAL CHARACTERISATION AND ORIENTATION OF CELL WALL POLYMERS IN MAIZE LEAVES
SP  - 551-554
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1811
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Pantić, Nevena and Stevanić, Jasna and Đikanović, Daniela and Mitrović, Aleksandra Lj. and Salmén, Lennart and Radotić, Ksenija",
year = "2019",
abstract = "Cell wall can be considered as a nano-composite in which cellulose, lignin and hemicelluloses are
interconnected in a specific manner. Mechanical and physical propreties of plant fibres are dependent
on the orientation of constituent polymers (cellulose, hemicellulose, lignin). Fourier transform
infrared (FTIR) microscopy was used to examine the orientation of the main plant polymers in
transversal and longitudinal direction of the isolated cell wall of the maize leaves. Polarised FTIR
measurements indicated an anisotropy, i.e. orientation of the cellulose microfibrils that was more or
less parallel to the longitudinal axis of the cell wall. Xylan has parallel orientation with regard to the
orientation of cellulose, as well as lignin.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth & Environmental Research",
title = "STRUCTURAL CHARACTERISATION AND ORIENTATION OF CELL WALL POLYMERS IN MAIZE LEAVES",
pages = "551-554",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1811"
}

Simonović Radosavljević, J., Pantić, N., Stevanić, J., Đikanović, D., Mitrović, A. Lj., Salmén, L.,& Radotić, K.. (2019). STRUCTURAL CHARACTERISATION AND ORIENTATION OF CELL WALL POLYMERS IN MAIZE LEAVES. in 27th International Conference Ecological Truth & Environmental Research
University of Belgrade, Technical Faculty in Bor., 551-554.
https://hdl.handle.net/21.15107/rcub_rimsi_1811

Simonović Radosavljević J, Pantić N, Stevanić J, Đikanović D, Mitrović AL, Salmén L, Radotić K. STRUCTURAL CHARACTERISATION AND ORIENTATION OF CELL WALL POLYMERS IN MAIZE LEAVES. in 27th International Conference Ecological Truth & Environmental Research. 2019;:551-554.
https://hdl.handle.net/21.15107/rcub_rimsi_1811 .

Simonović Radosavljević, Jasna, Pantić, Nevena, Stevanić, Jasna, Đikanović, Daniela, Mitrović, Aleksandra Lj., Salmén, Lennart, Radotić, Ksenija, "STRUCTURAL CHARACTERISATION AND ORIENTATION OF CELL WALL POLYMERS IN MAIZE LEAVES" in 27th International Conference Ecological Truth & Environmental Research (2019):551-554,
https://hdl.handle.net/21.15107/rcub_rimsi_1811 .

TY  - CONF
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1655
AB  - Steady state fluorescence spectroscopy in combination with Principal Component Analysis (PCA) for
spectral analysis was used to differentiate multifloral honeys from different parts of Serbia. The
emission spectra were recorded in the wavelength range 280 – 550 nm, after excitation in the 270 –
370 nm range. After normalization of the spectra, chemometric evaluation of the spectral data was
carried out using principal component analysis (PCA). This study indicates that front-face
fluorescence spectroscopy is a promising technique for the authentication of geographical origin of
honey and may also be useful for determination of the botanical origin within the same unifloral honey
type.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION
SP  - 89-92
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1655
ER  - 

@conference{
author = "Stanković, Mira and Bartolić, Dragana and Prokopijević, Miloš and Prodanović, Olivera and Đikanović, Daniela and Simonović Radosavljević, Jasna and Radotić, Ksenija",
year = "2019",
abstract = "Steady state fluorescence spectroscopy in combination with Principal Component Analysis (PCA) for
spectral analysis was used to differentiate multifloral honeys from different parts of Serbia. The
emission spectra were recorded in the wavelength range 280 – 550 nm, after excitation in the 270 –
370 nm range. After normalization of the spectra, chemometric evaluation of the spectral data was
carried out using principal component analysis (PCA). This study indicates that front-face
fluorescence spectroscopy is a promising technique for the authentication of geographical origin of
honey and may also be useful for determination of the botanical origin within the same unifloral honey
type.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION",
pages = "89-92",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1655"
}

Stanković, M., Bartolić, D., Prokopijević, M., Prodanović, O., Đikanović, D., Simonović Radosavljević, J.,& Radotić, K.. (2019). FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_1655

Stanković M, Bartolić D, Prokopijević M, Prodanović O, Đikanović D, Simonović Radosavljević J, Radotić K. FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION. in 27th International Conference Ecological Truth and Environmental Research. 2019;:89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_1655 .

Stanković, Mira, Bartolić, Dragana, Prokopijević, Miloš, Prodanović, Olivera, Đikanović, Daniela, Simonović Radosavljević, Jasna, Radotić, Ksenija, "FLUORESCENCE SPECTROSCOPY AND PRINCIPAL COMPONENT ANALYSIS IN THE HONEY SAMPLES CLASSIFICATION" in 27th International Conference Ecological Truth and Environmental Research (2019):89-92,
https://hdl.handle.net/21.15107/rcub_rimsi_1655 .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Stevanić, Jasna
AU  - Đikanović, Daniela
AU  - Mitrović, Aleksandra Lj.
AU  - Salmén, Lennart
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1758
AB  - Plant cell walls are composed of a framework of cellulose microfibrils that are
interconnected with heteropolysaccharides (lignin, hemicelluloses) in a specific
manner. Plant cell walls form a large part of the plant body and define its
characteristics. Structural organisation of the cell wall and related polymers is
important for both mechanical properties of plants and chemical reactions occurring
in the wall space, especially in the response to stress.By using imaging FTIR
microscopy, run in transmission mode and at different polarisation modes (from 0° to
90°), it is possible to follow the chemical variability and the orientation of cell wall
polymers (cellulose, hemicelluloses and lignin) of the Arabidopsis thaliana stem. The
polarised FTIR measurements indicated that both xylan and lignin have parallel
orientation with regard to the orientation of cellulose. It is believed that this structuring
of lignin in the S2 layer of the cell wall might be a result of the spatial constraints
within the cell wall, occuring due to the previous deposition of
cellulose/hemicellulose in a strongly oriented assembly.
PB  - Insitute for Nature Conservation of Serbia
PB  - Faculty of Science and Mathematics, Niš
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
T1  - Structural characterisation and orientation of cell wall polymers in Arabidopsis thaliana stem
SP  - 110-110
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1758
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Stevanić, Jasna and Đikanović, Daniela and Mitrović, Aleksandra Lj. and Salmén, Lennart and Radotić, Ksenija",
year = "2019",
abstract = "Plant cell walls are composed of a framework of cellulose microfibrils that are
interconnected with heteropolysaccharides (lignin, hemicelluloses) in a specific
manner. Plant cell walls form a large part of the plant body and define its
characteristics. Structural organisation of the cell wall and related polymers is
important for both mechanical properties of plants and chemical reactions occurring
in the wall space, especially in the response to stress.By using imaging FTIR
microscopy, run in transmission mode and at different polarisation modes (from 0° to
90°), it is possible to follow the chemical variability and the orientation of cell wall
polymers (cellulose, hemicelluloses and lignin) of the Arabidopsis thaliana stem. The
polarised FTIR measurements indicated that both xylan and lignin have parallel
orientation with regard to the orientation of cellulose. It is believed that this structuring
of lignin in the S2 layer of the cell wall might be a result of the spatial constraints
within the cell wall, occuring due to the previous deposition of
cellulose/hemicellulose in a strongly oriented assembly.",
publisher = "Insitute for Nature Conservation of Serbia, Faculty of Science and Mathematics, Niš",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions",
title = "Structural characterisation and orientation of cell wall polymers in Arabidopsis thaliana stem",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1758"
}

Simonović Radosavljević, J., Stevanić, J., Đikanović, D., Mitrović, A. Lj., Salmén, L.,& Radotić, K.. (2019). Structural characterisation and orientation of cell wall polymers in Arabidopsis thaliana stem. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions
Insitute for Nature Conservation of Serbia., 110-110.
https://hdl.handle.net/21.15107/rcub_rimsi_1758

Simonović Radosavljević J, Stevanić J, Đikanović D, Mitrović AL, Salmén L, Radotić K. Structural characterisation and orientation of cell wall polymers in Arabidopsis thaliana stem. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions. 2019;:110-110.
https://hdl.handle.net/21.15107/rcub_rimsi_1758 .

Simonović Radosavljević, Jasna, Stevanić, Jasna, Đikanović, Daniela, Mitrović, Aleksandra Lj., Salmén, Lennart, Radotić, Ksenija, "Structural characterisation and orientation of cell wall polymers in Arabidopsis thaliana stem" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions (2019):110-110,
https://hdl.handle.net/21.15107/rcub_rimsi_1758 .