TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Celine
AU  - Stupar, Petar
AU  - Villalba, Maria, I
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pesic, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1338
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
PB  - Wiley, Hoboken
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
IS  - 12
VL  - 33
DO  - 10.1002/jmr.2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Celine and Stupar, Petar and Villalba, Maria, I and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pesic, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
publisher = "Wiley, Hoboken",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
number = "12",
volume = "33",
doi = "10.1002/jmr.2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pesic, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition
Wiley, Hoboken., 33(12).
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pesic M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;33(12).
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Celine, Stupar, Petar, Villalba, Maria, I, Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pesic, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition, 33, no. 12 (2020),
https://doi.org/10.1002/jmr.2849 . .

TY  - JOUR
AU  - Kasas, Sandor
AU  - Radotić, Ksenija
AU  - Longo, Giovanni
AU  - Saha, Bashkar
AU  - Alonso-Sarduy, Livan
AU  - Dietler, Giovanni
AU  - Roduit, Charles
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/723
AB  - Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357363, 2013.
PB  - Wiley-Blackwell, Hoboken
T2  - Microscopy Research and Technique
T1  - A universal fluid cell for the imaging of biological specimens in the atomic force microscope
EP  - 363
IS  - 4
SP  - 357
VL  - 76
DO  - 10.1002/jemt.22174
ER  - 

@article{
author = "Kasas, Sandor and Radotić, Ksenija and Longo, Giovanni and Saha, Bashkar and Alonso-Sarduy, Livan and Dietler, Giovanni and Roduit, Charles",
year = "2013",
abstract = "Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357363, 2013.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Microscopy Research and Technique",
title = "A universal fluid cell for the imaging of biological specimens in the atomic force microscope",
pages = "363-357",
number = "4",
volume = "76",
doi = "10.1002/jemt.22174"
}

Kasas, S., Radotić, K., Longo, G., Saha, B., Alonso-Sarduy, L., Dietler, G.,& Roduit, C.. (2013). A universal fluid cell for the imaging of biological specimens in the atomic force microscope. in Microscopy Research and Technique
Wiley-Blackwell, Hoboken., 76(4), 357-363.
https://doi.org/10.1002/jemt.22174

Kasas S, Radotić K, Longo G, Saha B, Alonso-Sarduy L, Dietler G, Roduit C. A universal fluid cell for the imaging of biological specimens in the atomic force microscope. in Microscopy Research and Technique. 2013;76(4):357-363.
doi:10.1002/jemt.22174 .

Kasas, Sandor, Radotić, Ksenija, Longo, Giovanni, Saha, Bashkar, Alonso-Sarduy, Livan, Dietler, Giovanni, Roduit, Charles, "A universal fluid cell for the imaging of biological specimens in the atomic force microscope" in Microscopy Research and Technique, 76, no. 4 (2013):357-363,
https://doi.org/10.1002/jemt.22174 . .