TY  - JOUR
AU  - Ilijin, Larisa
AU  - Nikolić, Maria Vesna
AU  - Vasiljević, Zorka Z
AU  - Todorović, Dajana
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Matić, Dragana
AU  - Tadic, Nenad
AU  - Perić-Mataruga, Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3202
AB  - In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap
SP  - 131731
VL  - 268
DO  - 10.1016/j.ijbiomac.2024.131731
ER  - 

@article{
author = "Ilijin, Larisa and Nikolić, Maria Vesna and Vasiljević, Zorka Z and Todorović, Dajana and Mrdaković, Marija and Vlahović, Milena and Matić, Dragana and Tadic, Nenad and Perić-Mataruga, Vesna",
year = "2024",
abstract = "In this work we have characterized and compared chitin sourced from exoskeleton of Tenebrio molitor larvae fed with polystyrene or plastic kitchen wrap combined with bran in the ratio 1:1 with chitin sourced from larvae exoskeleton fed only with bran. Analysis of the frass by ATR-FTIR showed very similar spectra and confirmed degradation of the plastic feed components, while ATR-FTIR analysis of the exoskeleton verified the absence of any plastic residue. Deproteinization followed by demineralization produced 6.78 -5.29 % chitin, showing that plastic (polystyrene or plastic kitchen wrap) in the larvae diet resulted in heavier insect exoskeleton, but yielded slightly less chitin, with the lowest value obtained for plastic kitchen wrap in the insect diet. The deacetylation degree of 98.17-98.61% was determined from measured ATR-FTIR spectra. XRD analysis confirmed the presence of -chitin with a crystallinity index of 66.5-62% and crystallite size 4-5 nm. Thermogravimetric analysis showed similar degradation curves for all chitin samples, with two degradation steps. These results show that chitin sourced from exoskeleton of T. molitor larvae fed with plastic (polystyrene or plastic kitchen wrap) and contributing to significant biodegradation of major polluting materials can be a feasible and alternative source of chitin, further promoting a bio-circular economy.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap",
pages = "131731",
volume = "268",
doi = "10.1016/j.ijbiomac.2024.131731"
}

Ilijin, L., Nikolić, M. V., Vasiljević, Z. Z., Todorović, D., Mrdaković, M., Vlahović, M., Matić, D., Tadic, N.,& Perić-Mataruga, V.. (2024). Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules
Elsevier., 268, 131731.
https://doi.org/10.1016/j.ijbiomac.2024.131731

Ilijin L, Nikolić MV, Vasiljević ZZ, Todorović D, Mrdaković M, Vlahović M, Matić D, Tadic N, Perić-Mataruga V. Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap. in International Journal of Biological Macromolecules. 2024;268:131731.
doi:10.1016/j.ijbiomac.2024.131731 .

Ilijin, Larisa, Nikolić, Maria Vesna, Vasiljević, Zorka Z, Todorović, Dajana, Mrdaković, Marija, Vlahović, Milena, Matić, Dragana, Tadic, Nenad, Perić-Mataruga, Vesna, "Sourcing Chitin from Exoskeleton of Tenebrio molitor Fed with Polystyrene or Plastic Kitchen Wrap" in International Journal of Biological Macromolecules, 268 (2024):131731,
https://doi.org/10.1016/j.ijbiomac.2024.131731 . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Poleksic, Vesna
AU  - Tasic, Aleksandra
AU  - Smederevac-Lalić, Marija
AU  - Đikanović, Vesna
AU  - Raskovic, Bozidar
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2668
AB  - Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.
PB  - MDPI
T2  - Sustainability
T1  - Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution
IS  - 11321
VL  - 15
DO  - 10.3390/su151411321
ER  - 

@article{
author = "Nikolić, Dušan and Poleksic, Vesna and Tasic, Aleksandra and Smederevac-Lalić, Marija and Đikanović, Vesna and Raskovic, Bozidar",
year = "2023",
abstract = "Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.",
publisher = "MDPI",
journal = "Sustainability",
title = "Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution",
number = "11321",
volume = "15",
doi = "10.3390/su151411321"
}

Nikolić, D., Poleksic, V., Tasic, A., Smederevac-Lalić, M., Đikanović, V.,& Raskovic, B.. (2023). Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution. in Sustainability
MDPI., 15(11321).
https://doi.org/10.3390/su151411321

Nikolić D, Poleksic V, Tasic A, Smederevac-Lalić M, Đikanović V, Raskovic B. Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution. in Sustainability. 2023;15(11321).
doi:10.3390/su151411321 .

Nikolić, Dušan, Poleksic, Vesna, Tasic, Aleksandra, Smederevac-Lalić, Marija, Đikanović, Vesna, Raskovic, Bozidar, "Two Age Groups of Adult Pikeperch (Sander lucioperca) as Bioindicators of Aquatic Pollution" in Sustainability, 15, no. 11321 (2023),
https://doi.org/10.3390/su151411321 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2216
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.
PB  - Springer Nature
T2  - Springer Nature, Ecotoxicology 32, 583–597 (2023)
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
EP  - 597
IS  - 32
SP  - 583
DO  - https://doi.org/10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.",
publisher = "Springer Nature",
journal = "Springer Nature, Ecotoxicology 32, 583–597 (2023)",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
pages = "597-583",
number = "32",
doi = "https://doi.org/10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023)
Springer Nature.(32), 583-597.
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023). 2023;(32):583-597.
doi:https://doi.org/10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Springer Nature, Ecotoxicology 32, 583–597 (2023), no. 32 (2023):583-597,
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - CONF
AU  - Milosevic, Ana
AU  - Milosevic, Katarina
AU  - Nikolic, Ljiljana
AU  - Bogdanović Pristov, Jelena
AU  - Božić, Iva
AU  - Zivkovic, Anica
AU  - Lavrnja, Irena
AU  - Savic, Danijela
AU  - Janjic, Marija
AU  - Bjelobaba, Ivana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2019
AB  - Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls 
mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary 
gonadotrope cells.  While GnRHR signaling in gonadotropes is well described, 
knowledge of GnRHR activation-related events at extrapituitary sites including 
neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct 
changes in hippocampal gene expression, emotional processes, and cognitive 
functions.  
To explore neuronal GnRHR signaling we used the human neuroblastoma cell line 
SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and 
investigated the expression of several relevant genes in the hippocampus and preoptic 
area of peripubertal male rats treated with GnRHa. 
GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after 
adding GnRHa in the incubation media. Electrophysiological recordings confirmed 
that GnRHa induced membrane depolarization but could not evoke action potentials. 
In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, 
and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal 
rats with GnRHa had no effect on the expression of genes in the hippocampus 
previously shown to be affected in the sheep model of delayed puberty.  
These results imply that neuronal GnRHR is either differently coupled (not coupled 
with Gq/11 protein), or that its membrane density is too low to induce transcriptional 
events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR 
signaling in the brain.
PB  - Belgrade: Serbian Neuroscience Society
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - GnRHR signaling in neuronal cells: in vitro and in vivo data
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2019
ER  - 

@conference{
author = "Milosevic, Ana and Milosevic, Katarina and Nikolic, Ljiljana and Bogdanović Pristov, Jelena and Božić, Iva and Zivkovic, Anica and Lavrnja, Irena and Savic, Danijela and Janjic, Marija and Bjelobaba, Ivana",
year = "2023",
abstract = "Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls 
mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary 
gonadotrope cells.  While GnRHR signaling in gonadotropes is well described, 
knowledge of GnRHR activation-related events at extrapituitary sites including 
neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct 
changes in hippocampal gene expression, emotional processes, and cognitive 
functions.  
To explore neuronal GnRHR signaling we used the human neuroblastoma cell line 
SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and 
investigated the expression of several relevant genes in the hippocampus and preoptic 
area of peripubertal male rats treated with GnRHa. 
GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after 
adding GnRHa in the incubation media. Electrophysiological recordings confirmed 
that GnRHa induced membrane depolarization but could not evoke action potentials. 
In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, 
and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal 
rats with GnRHa had no effect on the expression of genes in the hippocampus 
previously shown to be affected in the sheep model of delayed puberty.  
These results imply that neuronal GnRHR is either differently coupled (not coupled 
with Gq/11 protein), or that its membrane density is too low to induce transcriptional 
events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR 
signaling in the brain.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "GnRHR signaling in neuronal cells: in vitro and in vivo data",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2019"
}

Milosevic, A., Milosevic, K., Nikolic, L., Bogdanović Pristov, J., Božić, I., Zivkovic, A., Lavrnja, I., Savic, D., Janjic, M.,& Bjelobaba, I.. (2023). GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Belgrade: Serbian Neuroscience Society., 53.
https://hdl.handle.net/21.15107/rcub_rimsi_2019

Milosevic A, Milosevic K, Nikolic L, Bogdanović Pristov J, Božić I, Zivkovic A, Lavrnja I, Savic D, Janjic M, Bjelobaba I. GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:53.
https://hdl.handle.net/21.15107/rcub_rimsi_2019 .

Milosevic, Ana, Milosevic, Katarina, Nikolic, Ljiljana, Bogdanović Pristov, Jelena, Božić, Iva, Zivkovic, Anica, Lavrnja, Irena, Savic, Danijela, Janjic, Marija, Bjelobaba, Ivana, "GnRHR signaling in neuronal cells: in vitro and in vivo data" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):53,
https://hdl.handle.net/21.15107/rcub_rimsi_2019 .

TY  - CONF
AU  - Jovanović, Mirna
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka M
AU  - Djordjevic, Ana
AU  - Brkljacic, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3059
AB  - Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.
PB  - Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
C3  - Belgrade: Faculty of Chemistry
T1  - Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes
EP  - 132
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3059
ER  - 

@conference{
author = "Jovanović, Mirna and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka M and Djordjevic, Ana and Brkljacic, Jelena",
year = "2023",
abstract = "Insulin resistance is a state where a normal amount of insulin can’t provoke an appropriate metabolic response. Insulin promotes membrane trafficking of the glucose transporter GLUT4 from the storage vesicles to the plasma membrane in white adipose tissue. Adipocytes use glucose for lipogenesis and store the energy as lipid droplets. If adipocytes are unable to uptake glucose, a chronic state of hyperglycemia is developed, with severe health consequences. Polyphenols are natural anti-inflammatory and antioxidant agents. Food rich in polyphenols has been suggested to exert an ameliorative effect on restoring insulin sensitivity, with the main identified target being AMPK1,2, one of the key sensors of intracellular energy. Here, we tested the effect of methanol extracts from three newly introduced strawberry (Fragaria x ananassa, Duch.) cultivars – 'Aprika', 'Sandra' and 'Quicky' on glucose metabolism in 3T3-F442A adipocytes. It was determined that 'Aprika' has the highest total phenolic content, relative to the other two cultivars. After 72-h exposure, none of the strawberry cultivars affected adipocyte cell growth significantly. Protein expression analysis of the differentiated adipocytes suggested 'Aprika', but not the other two cultivars, significantly increased the AMPK expression, as well as GLUT4, thus increasing glucose uptake. Strawberry extracts did not significantly affect the differentiation of adipocytes (SIRT1 and PPARγ), nor the fatty acid synthesis (ACC). Conclusively, the 'Aprika' methanol extract with high phenolic content exerts an ameliorative effect on glucose uptake, presumably through activation of the AMPK-dependent mechanism of GLUT4 trafficking. The systemic effects of the ‘Aprika’ cultivar should be further investigated. Implications of the research are decreased hyperglycemia in obese and diabetic patients, by the introduction of the 'Aprika' strawberry cultivar into everyday diet. References 1. Zhao L, Zou T, Gomez NA et al. Raspberry alleviates obesity-induced inflammation and insulin resistance in skeletal muscle through activation of AMP-activated protein kinase (AMPK) α1. Nutr & Diabetes, 2018, 8, 39. 2. Xu W, Luo Y, Yin J, Luo F. Targeting AMPK signaling by polyphenols: A novel strategy for tackling aging. Food & Function. 2023.",
publisher = "Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
journal = "Belgrade: Faculty of Chemistry",
title = "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes",
pages = "132-131",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3059"
}

Jovanović, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J. M., Djordjevic, A.,& Brkljacic, J.. (2023). Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Belgrade: Faculty of Chemistry
Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia., 131-132.
https://hdl.handle.net/21.15107/rcub_rimsi_3059

Jovanović M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević JM, Djordjevic A, Brkljacic J. Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes. in Belgrade: Faculty of Chemistry. 2023;:131-132.
https://hdl.handle.net/21.15107/rcub_rimsi_3059 .

Jovanović, Mirna, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka M, Djordjevic, Ana, Brkljacic, Jelena, "Methanol extract of strawberry cultivar 'Aprika' increases glucose uptake in 3T3-F442A adipocytes" in Belgrade: Faculty of Chemistry (2023):131-132,
https://hdl.handle.net/21.15107/rcub_rimsi_3059 .

TY  - CONF
AU  - Đikanović, Vesna
AU  - Jovicic, Katarina
AU  - Subotić, Srđan
AU  - Jaćimović, Milica
AU  - Nikolić, Dušan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2674
AB  - Lepomis gibbosus (pumpkinseed) was first recorded as a non-native fish in Serbia in the
1930s in the Tisza River. The species currently inhabits almost the entire region of the Black
Sea basin in Serbia, but has no economic value. The current range of the pumpkinseed
covers more than 50% of the total Serbian area and is also naturalised in Serbian waters.
The pumpkinseed feeds on bottom fauna, small fish, fish roe and juvenile fish, which
together with competition for habitat and spawning sites can have a negative impact on
native species. The most common prey of younger specimens is zooplankton, while adult
specimens feed on larger organisms such as larvae and pupae of insects, molluscs or
crustaceans. The composition of the prey varies seasonally and depends on the
composition of the community. The main objective of the study was to analyse the
differences in the feeding habits of pumpkinseed in five reservoir.
Samples were collected from five reservoirs in central Serbia (<200 asl, Markovačko,
Topola, Vlaški Do, Jatagan, Kudreč) using standard electrofishing equipment. All sampled
reservoirs had mud substrate, except Jatagan Reservoir, which had gravel substrate. The
sampled fish were identified on site based on morphological characteristics using
identification keys. The sampled fish were measured using total body length and weight.
The total sample consisted of 354 pumpkinseed individuals whose body weight ranged
from 3.66–9.52 g and body length from 6.01–8.41 cm. The number of individuals with
intestinal content was 282, while the number of individuals with empty stomachs was 72.
The following macroinvertebrate taxa were identified during the examination of the gut
contents: aquatic insects (Chironomidae, Chironomidae-pupae, Diptera, Ceratopogonidae,
Trichoptera, Ephemeroptera, Odonata, Heteroptera, Coleoptera), Gammaridae, Mollusca,
Bivalvia, Gastropoda, Nematoda. Undetermined terrestrial insects were also recorded. Most
prey were found in the Kudreč Reservoir, and the greatest diversity was in the Topola
Reservoir. Jatagan Reservoir had the lowest number of prey items, which may be due to
the substrate type. The high number of aquatic insect taxa could be explained by the small
surface area of the reservoir and the presence of aquatic vegetation.
PB  - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS), East and South European Network for Invasive Alien Species (ESENIAS), Danube Region Invasive Alien Species Network (DIAS)
C3  - Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop
T1  - Intestinal content analyses of pumpkinseed Lepomis gibbosus (Linnaeus, 1785) in five small reservoirs – central Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2674
ER  - 

@conference{
author = "Đikanović, Vesna and Jovicic, Katarina and Subotić, Srđan and Jaćimović, Milica and Nikolić, Dušan",
year = "2023",
abstract = "Lepomis gibbosus (pumpkinseed) was first recorded as a non-native fish in Serbia in the
1930s in the Tisza River. The species currently inhabits almost the entire region of the Black
Sea basin in Serbia, but has no economic value. The current range of the pumpkinseed
covers more than 50% of the total Serbian area and is also naturalised in Serbian waters.
The pumpkinseed feeds on bottom fauna, small fish, fish roe and juvenile fish, which
together with competition for habitat and spawning sites can have a negative impact on
native species. The most common prey of younger specimens is zooplankton, while adult
specimens feed on larger organisms such as larvae and pupae of insects, molluscs or
crustaceans. The composition of the prey varies seasonally and depends on the
composition of the community. The main objective of the study was to analyse the
differences in the feeding habits of pumpkinseed in five reservoir.
Samples were collected from five reservoirs in central Serbia (<200 asl, Markovačko,
Topola, Vlaški Do, Jatagan, Kudreč) using standard electrofishing equipment. All sampled
reservoirs had mud substrate, except Jatagan Reservoir, which had gravel substrate. The
sampled fish were identified on site based on morphological characteristics using
identification keys. The sampled fish were measured using total body length and weight.
The total sample consisted of 354 pumpkinseed individuals whose body weight ranged
from 3.66–9.52 g and body length from 6.01–8.41 cm. The number of individuals with
intestinal content was 282, while the number of individuals with empty stomachs was 72.
The following macroinvertebrate taxa were identified during the examination of the gut
contents: aquatic insects (Chironomidae, Chironomidae-pupae, Diptera, Ceratopogonidae,
Trichoptera, Ephemeroptera, Odonata, Heteroptera, Coleoptera), Gammaridae, Mollusca,
Bivalvia, Gastropoda, Nematoda. Undetermined terrestrial insects were also recorded. Most
prey were found in the Kudreč Reservoir, and the greatest diversity was in the Topola
Reservoir. Jatagan Reservoir had the lowest number of prey items, which may be due to
the substrate type. The high number of aquatic insect taxa could be explained by the small
surface area of the reservoir and the presence of aquatic vegetation.",
publisher = "Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS), East and South European Network for Invasive Alien Species (ESENIAS), Danube Region Invasive Alien Species Network (DIAS)",
journal = "Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop",
title = "Intestinal content analyses of pumpkinseed Lepomis gibbosus (Linnaeus, 1785) in five small reservoirs – central Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2674"
}

Đikanović, V., Jovicic, K., Subotić, S., Jaćimović, M.,& Nikolić, D.. (2023). Intestinal content analyses of pumpkinseed Lepomis gibbosus (Linnaeus, 1785) in five small reservoirs – central Serbia. in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS), East and South European Network for Invasive Alien Species (ESENIAS), Danube Region Invasive Alien Species Network (DIAS)..
https://hdl.handle.net/21.15107/rcub_rimsi_2674

Đikanović V, Jovicic K, Subotić S, Jaćimović M, Nikolić D. Intestinal content analyses of pumpkinseed Lepomis gibbosus (Linnaeus, 1785) in five small reservoirs – central Serbia. in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2674 .

Đikanović, Vesna, Jovicic, Katarina, Subotić, Srđan, Jaćimović, Milica, Nikolić, Dušan, "Intestinal content analyses of pumpkinseed Lepomis gibbosus (Linnaeus, 1785) in five small reservoirs – central Serbia" in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2674 .

TY  - CONF
AU  - Vitkovac, Aleksandra
AU  - Pajić, Tanja
AU  - Bukumira, Marta
AU  - Stanić, Marina
AU  - Rabasovic, Mihailo
AU  - Todorović, Nataša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2422
AB  - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023
C3  - 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
T1  - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
EP  - 337
SP  - 334
DO  - 10.46793/ICCBI23.334V
ER  - 

@conference{
author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasovic, Mihailo and Todorović, Nataša",
year = "2023",
abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023",
journal = "2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023",
title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology",
pages = "337-334",
doi = "10.46793/ICCBI23.334V"
}

Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasovic, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023., 334-337.
https://doi.org/10.46793/ICCBI23.334V

Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasovic M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023. 2023;:334-337.
doi:10.46793/ICCBI23.334V .

Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasovic, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 (2023):334-337,
https://doi.org/10.46793/ICCBI23.334V . .

TY  - JOUR
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Mićković, Branislav
AU  - Nikolić, Dušan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2617
AB  - The stomach content of 277 Amur sleeper individuals has been analyzed to present its
feeding habits. Fish were sampled using electrofishing from July to November 2017 in
the Danube River drainage channel near Veliko Gradište (Serbia). Fish age was
estimated by otoliths examination. Ingested prey organisms were identified to the
lowest reliable taxonomic level. The biotic indices: vacuity index (VI), frequency of
occurrence (F), abundance (Cn), index of importance (PV), Shannon’s diversity index
(H’), and equitability index (Eh) were calculated. A total of 18 prey categories and 1144
individual prey were identified in the fish diet, dominantly aquatic macroinvertebrates.
There were no significant difference in diet composition between sampling months as
well as 0+, 1+, and 2+ age groups (both 3+ and 4+ had one individual). Only eight
individuals were found with empty intestines, thus VI was low (2.9). In average, each
fish had 4.1 prey items in their intestines. For whole sample, H′ was 1.84 and Eh was
0.64. Trichoptera, Ephemeroptera, and Gastropoda were the most frequent,dominant, and abundant. The greatest diversity of prey items was recorded for fish sampled in October as well as for 2+ individuals, and the lowest for fish from August and 0+ individuals.
PB  - Central Fisheries Research Institute
T2  - Turkish Journal of Fisheries & Aquatic Sciences
T1  - Diet Analysis of the Amur Sleeper (Perccottus glenii) from the Danube River Drainage Channel (Serbia)
IS  - 12
VL  - 23
DO  - https://doi.org/10.4194/TRJFAS22854
ER  - 

@article{
author = "Đikanović, Vesna and Skorić, Stefan and Mićković, Branislav and Nikolić, Dušan",
year = "2023",
abstract = "The stomach content of 277 Amur sleeper individuals has been analyzed to present its
feeding habits. Fish were sampled using electrofishing from July to November 2017 in
the Danube River drainage channel near Veliko Gradište (Serbia). Fish age was
estimated by otoliths examination. Ingested prey organisms were identified to the
lowest reliable taxonomic level. The biotic indices: vacuity index (VI), frequency of
occurrence (F), abundance (Cn), index of importance (PV), Shannon’s diversity index
(H’), and equitability index (Eh) were calculated. A total of 18 prey categories and 1144
individual prey were identified in the fish diet, dominantly aquatic macroinvertebrates.
There were no significant difference in diet composition between sampling months as
well as 0+, 1+, and 2+ age groups (both 3+ and 4+ had one individual). Only eight
individuals were found with empty intestines, thus VI was low (2.9). In average, each
fish had 4.1 prey items in their intestines. For whole sample, H′ was 1.84 and Eh was
0.64. Trichoptera, Ephemeroptera, and Gastropoda were the most frequent,dominant, and abundant. The greatest diversity of prey items was recorded for fish sampled in October as well as for 2+ individuals, and the lowest for fish from August and 0+ individuals.",
publisher = "Central Fisheries Research Institute",
journal = "Turkish Journal of Fisheries & Aquatic Sciences",
title = "Diet Analysis of the Amur Sleeper (Perccottus glenii) from the Danube River Drainage Channel (Serbia)",
number = "12",
volume = "23",
doi = "https://doi.org/10.4194/TRJFAS22854"
}

Đikanović, V., Skorić, S., Mićković, B.,& Nikolić, D.. (2023). Diet Analysis of the Amur Sleeper (Perccottus glenii) from the Danube River Drainage Channel (Serbia). in Turkish Journal of Fisheries & Aquatic Sciences
Central Fisheries Research Institute., 23(12).
https://doi.org/https://doi.org/10.4194/TRJFAS22854

Đikanović V, Skorić S, Mićković B, Nikolić D. Diet Analysis of the Amur Sleeper (Perccottus glenii) from the Danube River Drainage Channel (Serbia). in Turkish Journal of Fisheries & Aquatic Sciences. 2023;23(12).
doi:https://doi.org/10.4194/TRJFAS22854 .

Đikanović, Vesna, Skorić, Stefan, Mićković, Branislav, Nikolić, Dušan, "Diet Analysis of the Amur Sleeper (Perccottus glenii) from the Danube River Drainage Channel (Serbia)" in Turkish Journal of Fisheries & Aquatic Sciences, 23, no. 12 (2023),
https://doi.org/https://doi.org/10.4194/TRJFAS22854 . .

TY  - CONF
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Tubić, Bojan
AU  - Jovičić, Katarina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2556
AB  - The Medjuvrsje reservoir is a highly modified water body on the Zapadna Morava River by the 
construction of a hydroelectric power plant. According to the national regulation it belongs to 
type 2 watercourses, i.e. large rivers with mineral substrates of medium grain size. The aim of 
this study was to evaluate the potential of the Medjuvrsje reservoir using bioindicative properties 
of benthic macroinvertebrates, taking into consideration their community composition. 
Macrozoobenthos were collected from two sites in the littoral zone of the reservoir using a 
standard hand net following the standard methodology (EN 27828). Specimens were sorted and 
identified under a stereo microscope using the appropriate identification keys. Assessment of the 
reservoir's ecological potential was carried out based on the class boundaries for Type 2 rivers 
using several biotic parameters calculated from AQEM (2002). The representatives of Oligochaeta, 
Mollusca, Odonata and Chironomidae were most abundant in the benthic community. The larval stages of the insects Ephemeroptera, Heteroptera, Coleoptera and Trichoptera were recorded only from the microhabitats with abundant macrophyte vegetation. Within this study, we also recorded the first finding of a freshwater sponge for this type of water body. Based on the macrozoobenthos 
composition and according to analyzed indices (saprobic index Zelinka & Marvan, BMWP and ASPT score, Diversity index Shannon-Weaner, Total number of taxa, share of Oligochaeta-Tubificidae) the ecological potential was assessed as good (II class of Water quality). However, when the EPT index was considered, the status deteriorated to IV (site 1) or V class
(site 2) as poor or bad status.
PB  - Hrvatskog udruženja slatkovodnih ekologa (HUSEk)
PB  - Biološki odsjek Prirodoslovno-matematičkog fakulteta (PMF) Sveučilišta u Zagrebu
C3  - 4th Symposium on Freshwater Biology
T1  - Assessment of ecological potential of the Medjuvrsje reservoir based on benthic macroinvertebrates
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2556
ER  - 

@conference{
author = "Đikanović, Vesna and Skorić, Stefan and Tubić, Bojan and Jovičić, Katarina",
year = "2023",
abstract = "The Medjuvrsje reservoir is a highly modified water body on the Zapadna Morava River by the 
construction of a hydroelectric power plant. According to the national regulation it belongs to 
type 2 watercourses, i.e. large rivers with mineral substrates of medium grain size. The aim of 
this study was to evaluate the potential of the Medjuvrsje reservoir using bioindicative properties 
of benthic macroinvertebrates, taking into consideration their community composition. 
Macrozoobenthos were collected from two sites in the littoral zone of the reservoir using a 
standard hand net following the standard methodology (EN 27828). Specimens were sorted and 
identified under a stereo microscope using the appropriate identification keys. Assessment of the 
reservoir's ecological potential was carried out based on the class boundaries for Type 2 rivers 
using several biotic parameters calculated from AQEM (2002). The representatives of Oligochaeta, 
Mollusca, Odonata and Chironomidae were most abundant in the benthic community. The larval stages of the insects Ephemeroptera, Heteroptera, Coleoptera and Trichoptera were recorded only from the microhabitats with abundant macrophyte vegetation. Within this study, we also recorded the first finding of a freshwater sponge for this type of water body. Based on the macrozoobenthos 
composition and according to analyzed indices (saprobic index Zelinka & Marvan, BMWP and ASPT score, Diversity index Shannon-Weaner, Total number of taxa, share of Oligochaeta-Tubificidae) the ecological potential was assessed as good (II class of Water quality). However, when the EPT index was considered, the status deteriorated to IV (site 1) or V class
(site 2) as poor or bad status.",
publisher = "Hrvatskog udruženja slatkovodnih ekologa (HUSEk), Biološki odsjek Prirodoslovno-matematičkog fakulteta (PMF) Sveučilišta u Zagrebu",
journal = "4th Symposium on Freshwater Biology",
title = "Assessment of ecological potential of the Medjuvrsje reservoir based on benthic macroinvertebrates",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2556"
}

Đikanović, V., Skorić, S., Tubić, B.,& Jovičić, K.. (2023). Assessment of ecological potential of the Medjuvrsje reservoir based on benthic macroinvertebrates. in 4th Symposium on Freshwater Biology
Hrvatskog udruženja slatkovodnih ekologa (HUSEk)..
https://hdl.handle.net/21.15107/rcub_rimsi_2556

Đikanović V, Skorić S, Tubić B, Jovičić K. Assessment of ecological potential of the Medjuvrsje reservoir based on benthic macroinvertebrates. in 4th Symposium on Freshwater Biology. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2556 .

Đikanović, Vesna, Skorić, Stefan, Tubić, Bojan, Jovičić, Katarina, "Assessment of ecological potential of the Medjuvrsje reservoir based on benthic macroinvertebrates" in 4th Symposium on Freshwater Biology (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2556 .

TY  - JOUR
AU  - Jovičić, Katarina
AU  - Janković, Saša
AU  - Nikolić, Dragica
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
AU  - Jarić, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2559
AB  - The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As.
The results suggest that scales and fins have a potential to be used as indicators of muscle tissue
contamination with As, Cu, and Hg, depending on species’ ecological traits.
PB  - EDP Sciences
T2  - Knowledge & Management of Aquatic Ecosystems
T1  - Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River
DO  - https://doi.org/10.1051/kmae/2022027
ER  - 

@article{
author = "Jovičić, Katarina and Janković, Saša and Nikolić, Dragica and Đikanović, Vesna and Skorić, Stefan and Krpo-Ćetković, Jasmina and Jarić, Ivan",
year = "2023",
abstract = "The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As.
The results suggest that scales and fins have a potential to be used as indicators of muscle tissue
contamination with As, Cu, and Hg, depending on species’ ecological traits.",
publisher = "EDP Sciences",
journal = "Knowledge & Management of Aquatic Ecosystems",
title = "Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River",
doi = "https://doi.org/10.1051/kmae/2022027"
}

Jovičić, K., Janković, S., Nikolić, D., Đikanović, V., Skorić, S., Krpo-Ćetković, J.,& Jarić, I.. (2023). Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River. in Knowledge & Management of Aquatic Ecosystems
EDP Sciences..
https://doi.org/https://doi.org/10.1051/kmae/2022027

Jovičić K, Janković S, Nikolić D, Đikanović V, Skorić S, Krpo-Ćetković J, Jarić I. Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River. in Knowledge & Management of Aquatic Ecosystems. 2023;.
doi:https://doi.org/10.1051/kmae/2022027 .

Jovičić, Katarina, Janković, Saša, Nikolić, Dragica, Đikanović, Vesna, Skorić, Stefan, Krpo-Ćetković, Jasmina, Jarić, Ivan, "Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River" in Knowledge & Management of Aquatic Ecosystems (2023),
https://doi.org/https://doi.org/10.1051/kmae/2022027 . .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Bogdanović Pristov, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2015
AB  - The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.
PB  - Serbian Neuroscience Society, Belgrade
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - Background norepinephrine impacts activity of cortical astrocytes
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2015
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bataveljic, Danijela and Bijelic, Dunja and Milicevic, Katarina and Bogdanović Pristov, Jelena",
year = "2023",
abstract = "The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "Background norepinephrine impacts activity of cortical astrocytes",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2015"
}

Nikolic, L., Bataveljic, D., Bijelic, D., Milicevic, K.,& Bogdanović Pristov, J.. (2023). Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society, Belgrade., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015

Nikolic L, Bataveljic D, Bijelic D, Milicevic K, Bogdanović Pristov J. Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

Nikolic, Ljiljana, Bataveljic, Danijela, Bijelic, Dunja, Milicevic, Katarina, Bogdanović Pristov, Jelena, "Background norepinephrine impacts activity of cortical astrocytes" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):51,
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2009
AB  - Astrocytes form a dense meshwork throughout the central nervous system (CNS) which qualifies them to perform interactive maintenance functions with neighboring cells. In neuroinflammation, this astroglial cell-to-cell interaction varies which can either promote or lessen pathological processes (1,2). In multiple sclerosis (MS), astrocytes engage in an interaction with immune cells which drives neurodegeneration by creating and sustaining an inflammatory CNS environment  (3).  Previously,  we  showed  that  CNS-infiltrated  immune  cells  (CNS-IICs)  in  the  experimental autoimmune  encephalomyelitis  (EAE)  rat,  rapidly  alter  the  activity  pattern  of  astrocytes  by  activating  glial  P2X7receptor  (P2X7R)(4).  In  the  present  study,  we  aimed  to  identify  the  properties of  astroglial  P2X7R  in  EAE  and  to identify mechanisms responsible for astrocyte activation in the presence of CNS-IICs (CD4+T cells). In this respect, spinal cords from rats at the peak of EAE and age-matched healthy controls were isolated and protein expression of P2X7R and connexin-43 (Cx-43) were investigated. P2X7R protein expression was decreased in the lumbar spinal cord, while Cx-43 did not change. Next, we found that P2X7R and Cx-43 proteins interact in the lumbar spinal cord since both the monomer and the dimer Cx-43 co-immunoprecipitate with P2X7R. Even though the colocalization of P2X7R and Cx-43 was decreased in EAE compared to the control, the analysis of the distribution of astroglial P2X7R and Cx-43 and their colocalization in the radius of 20 μm from the infiltrated CD4+T cell center showed that astroglial P2X7R and Cx-43 are specifically associated and concentrated in the proximity of CNS-IICs in the EAE spinal cord. Subsequently,  to  achieve  an  unambiguous  analysis  of  astrocyte-immune  cell  interaction,  we  monitored  Ca2+dynamics in Fluo-4 labeled cultured naïve astrocytes following brief bath-application of CNS-IICs isolated and purified from spinal cords of EAE rats. Our data suggest that astroglial αvβ3-integrin acted upstream of P2X7R activation and is likely involved in establishing initial contact of astrocytes with CNS-IICs since astrocytic αvβ3-integrin block reduced the astrocytic Ca2+response to CNS-IIC application. Furthermore, astrocytes challenged with CGP31157 (blocker of mNCLX and HCX) exhibited a prolonged intracellular Ca2+elevation and higher ATP release after brief exposure to CNS-IICs, indicating a regulatory function of mitochondria on this intracellular astrocyte Ca2+response. Collectively these  data  describing  integrin-relevant  cellular  mechanisms  of  astroglial  P2X7R  activation  could  help  to  expand integrin-inhibiting therapeutic approaches currently in use for MS treatment toward control ofastrocyte purine-based interaction with immune cells.
PB  - Wiley Periodicals LLC
C3  - XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet
T1  - P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells
SP  - E941
VL  - 71
DO  - https://doi.org/10.1002/glia.24419
ER  - 

@conference{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "Astrocytes form a dense meshwork throughout the central nervous system (CNS) which qualifies them to perform interactive maintenance functions with neighboring cells. In neuroinflammation, this astroglial cell-to-cell interaction varies which can either promote or lessen pathological processes (1,2). In multiple sclerosis (MS), astrocytes engage in an interaction with immune cells which drives neurodegeneration by creating and sustaining an inflammatory CNS environment  (3).  Previously,  we  showed  that  CNS-infiltrated  immune  cells  (CNS-IICs)  in  the  experimental autoimmune  encephalomyelitis  (EAE)  rat,  rapidly  alter  the  activity  pattern  of  astrocytes  by  activating  glial  P2X7receptor  (P2X7R)(4).  In  the  present  study,  we  aimed  to  identify  the  properties of  astroglial  P2X7R  in  EAE  and  to identify mechanisms responsible for astrocyte activation in the presence of CNS-IICs (CD4+T cells). In this respect, spinal cords from rats at the peak of EAE and age-matched healthy controls were isolated and protein expression of P2X7R and connexin-43 (Cx-43) were investigated. P2X7R protein expression was decreased in the lumbar spinal cord, while Cx-43 did not change. Next, we found that P2X7R and Cx-43 proteins interact in the lumbar spinal cord since both the monomer and the dimer Cx-43 co-immunoprecipitate with P2X7R. Even though the colocalization of P2X7R and Cx-43 was decreased in EAE compared to the control, the analysis of the distribution of astroglial P2X7R and Cx-43 and their colocalization in the radius of 20 μm from the infiltrated CD4+T cell center showed that astroglial P2X7R and Cx-43 are specifically associated and concentrated in the proximity of CNS-IICs in the EAE spinal cord. Subsequently,  to  achieve  an  unambiguous  analysis  of  astrocyte-immune  cell  interaction,  we  monitored  Ca2+dynamics in Fluo-4 labeled cultured naïve astrocytes following brief bath-application of CNS-IICs isolated and purified from spinal cords of EAE rats. Our data suggest that astroglial αvβ3-integrin acted upstream of P2X7R activation and is likely involved in establishing initial contact of astrocytes with CNS-IICs since astrocytic αvβ3-integrin block reduced the astrocytic Ca2+response to CNS-IIC application. Furthermore, astrocytes challenged with CGP31157 (blocker of mNCLX and HCX) exhibited a prolonged intracellular Ca2+elevation and higher ATP release after brief exposure to CNS-IICs, indicating a regulatory function of mitochondria on this intracellular astrocyte Ca2+response. Collectively these  data  describing  integrin-relevant  cellular  mechanisms  of  astroglial  P2X7R  activation  could  help  to  expand integrin-inhibiting therapeutic approaches currently in use for MS treatment toward control ofastrocyte purine-based interaction with immune cells.",
publisher = "Wiley Periodicals LLC",
journal = "XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet",
title = "P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells",
pages = "E941",
volume = "71",
doi = "https://doi.org/10.1002/glia.24419"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells. in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet
Wiley Periodicals LLC., 71, E941.
https://doi.org/https://doi.org/10.1002/glia.24419

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells. in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet. 2023;71:E941.
doi:https://doi.org/10.1002/glia.24419 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells" in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet, 71 (2023):E941,
https://doi.org/https://doi.org/10.1002/glia.24419 . .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2014
AB  - The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.
PB  - Serbian Neuroscience Society
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2014
ER  - 

@conference{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.",
publisher = "Serbian Neuroscience Society",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2014"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society., 101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):101,
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .

TY  - JOUR
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2007
AB  - In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.
PB  - MDPI
T2  - Cells
T1  - Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43
IS  - 13
SP  - 1786
VL  - 12
DO  - https://doi.org/10.3390/cells12131786
ER  - 

@article{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.",
publisher = "MDPI",
journal = "Cells",
title = "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43",
number = "13",
pages = "1786",
volume = "12",
doi = "https://doi.org/10.3390/cells12131786"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells
MDPI., 12(13), 1786.
https://doi.org/https://doi.org/10.3390/cells12131786

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells. 2023;12(13):1786.
doi:https://doi.org/10.3390/cells12131786 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43" in Cells, 12, no. 13 (2023):1786,
https://doi.org/https://doi.org/10.3390/cells12131786 . .

TY  - JOUR
AU  - SHEN, Weida
AU  - Bogdanović Pristov, Jelena
AU  - Nobili, Paola
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2004
AB  - Epilepsy is a neurological disorder caused by the pathological hyper-synchronization of neuronal discharges. The fundamental research of epilepsy mechanisms and the targets of drug design options for its treatment have focused on neurons. However, approximately 30% of patients suffering from epilepsy show resistance to standard anti-epileptic chemotherapeutic agents while the symptoms of the remaining 70% of patients can be alleviated but not completely removed by the current medications. Thus, new strategies for the treatment of epilepsy are in urgent demand. Over the past decades, with the increase in knowledge on the role of glia in the genesis and development of epilepsy, glial cells are receiving renewed attention. In a normal brain, glial cells maintain neuronal health and in partnership with neurons regulate virtually every aspect of brain function. In epilepsy, however, the supportive roles of glial cells are compromised, and their interaction with neurons is altered, which disrupts brain function. In this review, we will focus on the role of glia-related processes in epileptogenesis and their contribution to abnormal neuronal activity, with the major focus on the dysfunction of astroglial potassium channels, water channels, gap junctions, glutamate transporters, purinergic signaling, synaptogenesis, on the roles of microglial inflammatory cytokines, microglia-astrocyte interactions in epilepsy, and on the oligodendroglial potassium channels and myelin abnormalities in the epileptic brain. These recent findings suggest that glia should be considered as the promising next-generation targets for designing anti-epileptic drugs that may improve epilepsy and drug-resistant epilepsy.
PB  - Wolters Kluwer – Medknow
T2  - NEURAL REGENERATION RESEARCH
T1  - Can glial cells save neurons in epilepsy?
EP  - 1422
IS  - 7
SP  - 1417
VL  - 18
DO  - 10.4103/1673-5374.360281
ER  - 

@article{
author = "SHEN, Weida and Bogdanović Pristov, Jelena and Nobili, Paola and Nikolic, Ljiljana",
year = "2023",
abstract = "Epilepsy is a neurological disorder caused by the pathological hyper-synchronization of neuronal discharges. The fundamental research of epilepsy mechanisms and the targets of drug design options for its treatment have focused on neurons. However, approximately 30% of patients suffering from epilepsy show resistance to standard anti-epileptic chemotherapeutic agents while the symptoms of the remaining 70% of patients can be alleviated but not completely removed by the current medications. Thus, new strategies for the treatment of epilepsy are in urgent demand. Over the past decades, with the increase in knowledge on the role of glia in the genesis and development of epilepsy, glial cells are receiving renewed attention. In a normal brain, glial cells maintain neuronal health and in partnership with neurons regulate virtually every aspect of brain function. In epilepsy, however, the supportive roles of glial cells are compromised, and their interaction with neurons is altered, which disrupts brain function. In this review, we will focus on the role of glia-related processes in epileptogenesis and their contribution to abnormal neuronal activity, with the major focus on the dysfunction of astroglial potassium channels, water channels, gap junctions, glutamate transporters, purinergic signaling, synaptogenesis, on the roles of microglial inflammatory cytokines, microglia-astrocyte interactions in epilepsy, and on the oligodendroglial potassium channels and myelin abnormalities in the epileptic brain. These recent findings suggest that glia should be considered as the promising next-generation targets for designing anti-epileptic drugs that may improve epilepsy and drug-resistant epilepsy.",
publisher = "Wolters Kluwer – Medknow",
journal = "NEURAL REGENERATION RESEARCH",
title = "Can glial cells save neurons in epilepsy?",
pages = "1422-1417",
number = "7",
volume = "18",
doi = "10.4103/1673-5374.360281"
}

SHEN, W., Bogdanović Pristov, J., Nobili, P.,& Nikolic, L.. (2023). Can glial cells save neurons in epilepsy?. in NEURAL REGENERATION RESEARCH
Wolters Kluwer – Medknow., 18(7), 1417-1422.
https://doi.org/10.4103/1673-5374.360281

SHEN W, Bogdanović Pristov J, Nobili P, Nikolic L. Can glial cells save neurons in epilepsy?. in NEURAL REGENERATION RESEARCH. 2023;18(7):1417-1422.
doi:10.4103/1673-5374.360281 .

SHEN, Weida, Bogdanović Pristov, Jelena, Nobili, Paola, Nikolic, Ljiljana, "Can glial cells save neurons in epilepsy?" in NEURAL REGENERATION RESEARCH, 18, no. 7 (2023):1417-1422,
https://doi.org/10.4103/1673-5374.360281 . .

TY  - JOUR
AU  - Jovičić, Katarina
AU  - Janković, Saša
AU  - Nikolić, Dragica
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
AU  - Jarić, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1731
AB  - The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As. The results suggest that scales and fins have a potential to be used as indicators of muscle tissue contamination with As, Cu, and Hg, depending on species’ ecological traits.
PB  - EDP Sciences
T2  - Knowledge & Management of Aquatic Ecosystems
T1  - Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River
IS  - 4
VL  - 424
DO  - https://doi.org/10.1051/kmae/2022027
ER  - 

@article{
author = "Jovičić, Katarina and Janković, Saša and Nikolić, Dragica and Đikanović, Vesna and Skorić, Stefan and Krpo-Ćetković, Jasmina and Jarić, Ivan",
year = "2023",
abstract = "The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As. The results suggest that scales and fins have a potential to be used as indicators of muscle tissue contamination with As, Cu, and Hg, depending on species’ ecological traits.",
publisher = "EDP Sciences",
journal = "Knowledge & Management of Aquatic Ecosystems",
title = "Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River",
number = "4",
volume = "424",
doi = "https://doi.org/10.1051/kmae/2022027"
}

Jovičić, K., Janković, S., Nikolić, D., Đikanović, V., Skorić, S., Krpo-Ćetković, J.,& Jarić, I.. (2023). Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River. in Knowledge & Management of Aquatic Ecosystems
EDP Sciences., 424(4).
https://doi.org/https://doi.org/10.1051/kmae/2022027

Jovičić K, Janković S, Nikolić D, Đikanović V, Skorić S, Krpo-Ćetković J, Jarić I. Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River. in Knowledge & Management of Aquatic Ecosystems. 2023;424(4).
doi:https://doi.org/10.1051/kmae/2022027 .

Jovičić, Katarina, Janković, Saša, Nikolić, Dragica, Đikanović, Vesna, Skorić, Stefan, Krpo-Ćetković, Jasmina, Jarić, Ivan, "Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River" in Knowledge & Management of Aquatic Ecosystems, 424, no. 4 (2023),
https://doi.org/https://doi.org/10.1051/kmae/2022027 . .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Savic, Danijela
AU  - Korać Jačić, Jelena
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2010
AB  - Living cells exhibit multiple ion channel proteins and malfunction of these channels underlies 
numerous diseases. Channelopathies include diseases of the nervous, cardiovascular, respiratory, 
endocrine, urinary, and immune system. Currently, ion channels represent the second-largest target for existing drugs. Here, using the whole-cell patch-clamp technique, we explored the epinephrine effect on membrane ionic currents in glioma C6 cells. We demonstrate that epinephrine specifically evokes an increase of C6 cells outward ionic currents that is stable within 10 min, while it does not affect inward currents. Our results thus provide fine resolution and time frame for targeting ion channel activity that is crucial in pharmacological investigations.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION
EP  - 592
SP  - 589
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2010
ER  - 

@conference{
author = "Nikolic, Ljiljana and Savic, Danijela and Korać Jačić, Jelena and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Living cells exhibit multiple ion channel proteins and malfunction of these channels underlies 
numerous diseases. Channelopathies include diseases of the nervous, cardiovascular, respiratory, 
endocrine, urinary, and immune system. Currently, ion channels represent the second-largest target for existing drugs. Here, using the whole-cell patch-clamp technique, we explored the epinephrine effect on membrane ionic currents in glioma C6 cells. We demonstrate that epinephrine specifically evokes an increase of C6 cells outward ionic currents that is stable within 10 min, while it does not affect inward currents. Our results thus provide fine resolution and time frame for targeting ion channel activity that is crucial in pharmacological investigations.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION",
pages = "592-589",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2010"
}

Nikolic, L., Savic, D., Korać Jačić, J., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 2, 589-592.
https://hdl.handle.net/21.15107/rcub_rimsi_2010

Nikolic L, Savic D, Korać Jačić J, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;2:589-592.
https://hdl.handle.net/21.15107/rcub_rimsi_2010 .

Nikolic, Ljiljana, Savic, Danijela, Korać Jačić, Jelena, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 2 (2022):589-592,
https://hdl.handle.net/21.15107/rcub_rimsi_2010 .

TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Djordjevic, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1570
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
SP  - 9023
VL  - 23
DO  - 10.3390/ijms23169023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović Milutinović, Danijela and Blagojević, Duško and Djordjevic, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
pages = "9023",
volume = "23",
doi = "10.3390/ijms23169023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović Milutinović, D., Blagojević, D., Djordjevic, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović Milutinović D, Blagojević D, Djordjevic A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović Milutinović, Danijela, Blagojević, Duško, Djordjevic, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - CONF
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Mićković, Branislav
AU  - Nikolić, Dušan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1660
AB  - Ihtiofaunistička istraživanja obavljena su na osam vodotokova na području Specijalnog
rezervata prirode „Uvac”. Ukupno je registrovano prisustvo 15 vrsta iz 5 familija. Na osnovu
vrednosti primenjenog indeksa saprobnosti vodotoci Uvac, Vapa i Kladnicu su svrstani u I-II
klasu ekološkog statusa, Grabovica i Jablanica su II-III klasa, dok su Tisovica, Vrševina i
Zlošnica kategorisane kao I klasa ekološkog statusa. Istraživane tekućice odlikuje prisustvo
nativnih ihtiozajednica, što je od posebnog značaja za očuvanje originalnog diverziteta.
AB  - Ichthyofaunistic surveys were conducted on eight small streams in the area of Special natural
reserve ”Uvac”. A total of 15 species from 5 families have been identified. Based on the value
of the applied saprobity index, watercourses Uvac, Vapa and Kladnicu are classified as I-II
ecological status class, Grabovica and Jablanica are II-III class, while Tisovica, Vrševina and
Zlošnica are categorized as I ecological status class. Native fish communities characterize
investigated streams.
PB  - Српско друштво за заштиту вода, Београд
C3  - Voda 2022 - 51. godišnja konferencija o aktuelnim problemima korišćenja i zaštite voda
T1  - OCENA EKOLOŠKOG STATUSA TEKUĆICA ZAŠTIĆENOG PODRUČJA SRP „UVAC“ NA OSNOVU ZAJEDNICE RIBA
T1  - THE EVALUATION OF ECOLOGICAL STATUS OF SMALL MOUNTAIN- WATERS/STREAMS IN THE AREA OF RESERVE “UVAC” BASED ON FISH COMMUNITIES
EP  - 58
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1660
ER  - 

@conference{
author = "Đikanović, Vesna and Skorić, Stefan and Mićković, Branislav and Nikolić, Dušan",
year = "2022",
abstract = "Ihtiofaunistička istraživanja obavljena su na osam vodotokova na području Specijalnog
rezervata prirode „Uvac”. Ukupno je registrovano prisustvo 15 vrsta iz 5 familija. Na osnovu
vrednosti primenjenog indeksa saprobnosti vodotoci Uvac, Vapa i Kladnicu su svrstani u I-II
klasu ekološkog statusa, Grabovica i Jablanica su II-III klasa, dok su Tisovica, Vrševina i
Zlošnica kategorisane kao I klasa ekološkog statusa. Istraživane tekućice odlikuje prisustvo
nativnih ihtiozajednica, što je od posebnog značaja za očuvanje originalnog diverziteta., Ichthyofaunistic surveys were conducted on eight small streams in the area of Special natural
reserve ”Uvac”. A total of 15 species from 5 families have been identified. Based on the value
of the applied saprobity index, watercourses Uvac, Vapa and Kladnicu are classified as I-II
ecological status class, Grabovica and Jablanica are II-III class, while Tisovica, Vrševina and
Zlošnica are categorized as I ecological status class. Native fish communities characterize
investigated streams.",
publisher = "Српско друштво за заштиту вода, Београд",
journal = "Voda 2022 - 51. godišnja konferencija o aktuelnim problemima korišćenja i zaštite voda",
title = "OCENA EKOLOŠKOG STATUSA TEKUĆICA ZAŠTIĆENOG PODRUČJA SRP „UVAC“ NA OSNOVU ZAJEDNICE RIBA, THE EVALUATION OF ECOLOGICAL STATUS OF SMALL MOUNTAIN- WATERS/STREAMS IN THE AREA OF RESERVE “UVAC” BASED ON FISH COMMUNITIES",
pages = "58-53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1660"
}

Đikanović, V., Skorić, S., Mićković, B.,& Nikolić, D.. (2022). OCENA EKOLOŠKOG STATUSA TEKUĆICA ZAŠTIĆENOG PODRUČJA SRP „UVAC“ NA OSNOVU ZAJEDNICE RIBA. in Voda 2022 - 51. godišnja konferencija o aktuelnim problemima korišćenja i zaštite voda
Српско друштво за заштиту вода, Београд., 53-58.
https://hdl.handle.net/21.15107/rcub_rimsi_1660

Đikanović V, Skorić S, Mićković B, Nikolić D. OCENA EKOLOŠKOG STATUSA TEKUĆICA ZAŠTIĆENOG PODRUČJA SRP „UVAC“ NA OSNOVU ZAJEDNICE RIBA. in Voda 2022 - 51. godišnja konferencija o aktuelnim problemima korišćenja i zaštite voda. 2022;:53-58.
https://hdl.handle.net/21.15107/rcub_rimsi_1660 .

Đikanović, Vesna, Skorić, Stefan, Mićković, Branislav, Nikolić, Dušan, "OCENA EKOLOŠKOG STATUSA TEKUĆICA ZAŠTIĆENOG PODRUČJA SRP „UVAC“ NA OSNOVU ZAJEDNICE RIBA" in Voda 2022 - 51. godišnja konferencija o aktuelnim problemima korišćenja i zaštite voda (2022):53-58,
https://hdl.handle.net/21.15107/rcub_rimsi_1660 .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Mićković, Branislav
AU  - Nikčević, Miroslav
AU  - Smederevac-Lalić, Marija
AU  - Đikanović, Vesna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1528
AB  - Accumulation of Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was analyzed in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia) using inductively coupled plasma optical emission spectrometry (ICP-OES). The highest concentrations of Cu in liver as well as Ba, Ca, Fe, Mn, Na, P, and Sr in gills were detected in all fish species, while the lowest concentrations of Cu, Fe, Mn, Na, and Zn were detected in muscle. Pikeperch had significantly lower concentrations of Ba in all tissues compared to the other species, as well as Fe, P, and Sr in muscle, K, Mg, Mn, Sr, and Zn in gills, and Fe in gonads. European chub had significantly higher concentrations of Ca, Cu, and Zn in muscle, Cu in liver, and Cr and Cu in gonads compared to Cactus roach and pikeperch. Cd concentration in one individual of Cactus roach exceeded the maximum allowed concentration. The muscle was exposed to the lowest pressure of metal pollution in all species, and European chub accumulated more metals compared to the other species. There is no significant health risk due to consumption of investigated species. A major hazard index (HI) contributor in all species was Hg. According to the target carcinogenic risk factor (TR), target hazard quotient (THQ), and HI, men are under lower health risk compared to the women. The TR and THQ for As, Cr, Cu, Fe, Mn, Ni, and Zn as well as HI values were higher for European chub compared to the other species. On the other hand, THQ for Hg was highest for pikeperch.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Accumulation of 25 elements in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia)
DO  - 10.1007/s11356-022-19472-9
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Mićković, Branislav and Nikčević, Miroslav and Smederevac-Lalić, Marija and Đikanović, Vesna",
year = "2022",
abstract = "Accumulation of Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was analyzed in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia) using inductively coupled plasma optical emission spectrometry (ICP-OES). The highest concentrations of Cu in liver as well as Ba, Ca, Fe, Mn, Na, P, and Sr in gills were detected in all fish species, while the lowest concentrations of Cu, Fe, Mn, Na, and Zn were detected in muscle. Pikeperch had significantly lower concentrations of Ba in all tissues compared to the other species, as well as Fe, P, and Sr in muscle, K, Mg, Mn, Sr, and Zn in gills, and Fe in gonads. European chub had significantly higher concentrations of Ca, Cu, and Zn in muscle, Cu in liver, and Cr and Cu in gonads compared to Cactus roach and pikeperch. Cd concentration in one individual of Cactus roach exceeded the maximum allowed concentration. The muscle was exposed to the lowest pressure of metal pollution in all species, and European chub accumulated more metals compared to the other species. There is no significant health risk due to consumption of investigated species. A major hazard index (HI) contributor in all species was Hg. According to the target carcinogenic risk factor (TR), target hazard quotient (THQ), and HI, men are under lower health risk compared to the women. The TR and THQ for As, Cr, Cu, Fe, Mn, Ni, and Zn as well as HI values were higher for European chub compared to the other species. On the other hand, THQ for Hg was highest for pikeperch.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Accumulation of 25 elements in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia)",
doi = "10.1007/s11356-022-19472-9"
}

Nikolić, D., Skorić, S., Mićković, B., Nikčević, M., Smederevac-Lalić, M.,& Đikanović, V.. (2022). Accumulation of 25 elements in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg..
https://doi.org/10.1007/s11356-022-19472-9

Nikolić D, Skorić S, Mićković B, Nikčević M, Smederevac-Lalić M, Đikanović V. Accumulation of 25 elements in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia). in Environmental Science and Pollution Research. 2022;.
doi:10.1007/s11356-022-19472-9 .

Nikolić, Dušan, Skorić, Stefan, Mićković, Branislav, Nikčević, Miroslav, Smederevac-Lalić, Marija, Đikanović, Vesna, "Accumulation of 25 elements in gills, liver, gonads, and muscle of European chub (Squalius cephalus), Cactus roach (Rutilus virgo), and pikeperch (Sander lucioperca) from Zlatar reservoir (Serbia)" in Environmental Science and Pollution Research (2022),
https://doi.org/10.1007/s11356-022-19472-9 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2707
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.
PB  - MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - https://doi.org/10.3390/plants11243587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "https://doi.org/10.3390/plants11243587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
MDPI., 11(24).
https://doi.org/https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24).
doi:https://doi.org/10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022),
https://doi.org/https://doi.org/10.3390/plants11243587 . .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Vidović, Marija
AU  - Todorovic, Natasa
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2008
AB  - Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE
EP  - 274
SP  - 271
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2008
ER  - 

@conference{
author = "Nikolic, Ljiljana and Vidović, Marija and Todorovic, Natasa and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE",
pages = "274-271",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2008"
}

Nikolic, L., Vidović, M., Todorovic, N., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 1, 271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008

Nikolic L, Vidović M, Todorovic N, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;1:271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

Nikolic, Ljiljana, Vidović, Marija, Todorovic, Natasa, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 1 (2022):271-274,
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

TY  - JOUR
AU  - Kostić, Igor
AU  - Milanovic, Slobodan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Calic, Dusica D
AU  - Jankovsky, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2376
AB  - Botanicals, such as essential oils (EO) and their compounds, are considered a viable eco-friendly alternative to synthetic insecticides, which threaten human health and ecosystem functioning. In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid) and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology were assessed at different compound concentrations and compared with the effects of the commercial botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results suggest that anethole and carvone might be used as control agents against GML.
PB  - MDPI
T2  - Agronomy 2022, 12(12), 3049
T1  - Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)
VL  - 12
DO  - 10.3390/agronomy12123049
ER  - 

@article{
author = "Kostić, Igor and Milanovic, Slobodan and Kostic, Miroslav and Šešlija Jovanović, Darka and Calic, Dusica D and Jankovsky, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Botanicals, such as essential oils (EO) and their compounds, are considered a viable eco-friendly alternative to synthetic insecticides, which threaten human health and ecosystem functioning. In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid) and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology were assessed at different compound concentrations and compared with the effects of the commercial botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results suggest that anethole and carvone might be used as control agents against GML.",
publisher = "MDPI",
journal = "Agronomy 2022, 12(12), 3049",
title = "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)",
volume = "12",
doi = "10.3390/agronomy12123049"
}

Kostić, I., Milanovic, S., Kostic, M., Šešlija Jovanović, D., Calic, D. D., Jankovsky, L.,& Lazarević, J.. (2022). Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy 2022, 12(12), 3049
MDPI., 12.
https://doi.org/10.3390/agronomy12123049

Kostić I, Milanovic S, Kostic M, Šešlija Jovanović D, Calic DD, Jankovsky L, Lazarević J. Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy 2022, 12(12), 3049. 2022;12.
doi:10.3390/agronomy12123049 .

Kostić, Igor, Milanovic, Slobodan, Kostic, Miroslav, Šešlija Jovanović, Darka, Calic, Dusica D, Jankovsky, Libor, Lazarević, Jelica, "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)" in Agronomy 2022, 12(12), 3049, 12 (2022),
https://doi.org/10.3390/agronomy12123049 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović Milutinović, Danijela
AU  - Kirschner, Alexander K. T.
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Djukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1920
AB  - Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1920
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Jovanović Marić, Jovana and Vojnović Milutinović, Danijela and Kirschner, Alexander K. T. and Farnleitner, Andreas and Linke, Rita and Djukic, Aleksandar and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1920"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Jovanović Marić, J., Vojnović Milutinović, D., Kirschner, A. K. T., Farnleitner, A., Linke, R., Djukic, A., Kostić-Vuković, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)., 614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Jovanović Marić J, Vojnović Milutinović D, Kirschner AKT, Farnleitner A, Linke R, Djukic A, Kostić-Vuković J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Jovanović Marić, Jovana, Vojnović Milutinović, Danijela, Kirschner, Alexander K. T., Farnleitner, Andreas, Linke, Rita, Djukic, Aleksandar, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):614,
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .