@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović Milutinović, Danijela and Blagojević, Duško and Djordjevic, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
pages = "9023",
volume = "23",
doi = "10.3390/ijms23169023"
}
