Background norepinephrine impacts activity of cortical astrocytes
Само за регистроване кориснике
2023
Аутори
Nikolic, LjiljanaBataveljic, Danijela
Bijelic, Dunja
Milicevic, Katarina
Bogdanović Pristov, Jelena
Конференцијски прилог (Објављена верзија)
,
Serbian Neuroscience Society and associates
Метаподаци
Приказ свих података о документуАпстракт
The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal,
attention, cognitive function and stress responses. Unlike fast neurotransmitters which
act at synapses, NE is released in the neuropil and performs multiple targeting in the
surrounding area. The glial cells astrocytes are a direct target of NE, as they express
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial
responses elicited by strong and transient increases of NE in the brain are well studied,
but the effect of a low background NE concentration on astrocytes is unknown. This
background level of NE is maintained by basal noradrenergic activity and is
constantly present in the brain. Therefore, the response of astrocytes to the
background NE could have been unintentionally evoked in previous studies but its
effect overlooked.
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imag...ing and pharmacology. We used cultured
cortical astrocytes to bypass NE targeting of multiple cell types.
We show that cortical astrocytes detect and respond to the background NE
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from
intracellular stores further increased large-conductance, Ca2+-sensitive potassium
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat
cortex. Furthermore, stimulation of astrocytes by background NE was inhibited by
alpha-adrenoceptor antagonist.
Our results suggest that astrocytes maintain basal brain activity by perceiving and
responding to the background NE.
Кључне речи:
norepinephrine / astrocyte / BK channelИзвор:
8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS, 2023, 51-Издавач:
- Serbian Neuroscience Society, Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
Напомена:
- kategorija M64
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Nikolic, Ljiljana AU - Bataveljic, Danijela AU - Bijelic, Dunja AU - Milicevic, Katarina AU - Bogdanović Pristov, Jelena PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2015 AB - The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, attention, cognitive function and stress responses. Unlike fast neurotransmitters which act at synapses, NE is released in the neuropil and performs multiple targeting in the surrounding area. The glial cells astrocytes are a direct target of NE, as they express all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial responses elicited by strong and transient increases of NE in the brain are well studied, but the effect of a low background NE concentration on astrocytes is unknown. This background level of NE is maintained by basal noradrenergic activity and is constantly present in the brain. Therefore, the response of astrocytes to the background NE could have been unintentionally evoked in previous studies but its effect overlooked. To assess action of background NE on astrocytes we combined the whole-cell patch clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured cortical astrocytes to bypass NE targeting of multiple cell types. We show that cortical astrocytes detect and respond to the background NE concentration with an increase in intracellular Ca2+. This Ca2+ liberated from intracellular stores further increased large-conductance, Ca2+-sensitive potassium (BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat cortex. Furthermore, stimulation of astrocytes by background NE was inhibited by alpha-adrenoceptor antagonist. Our results suggest that astrocytes maintain basal brain activity by perceiving and responding to the background NE. PB - Serbian Neuroscience Society, Belgrade C3 - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS T1 - Background norepinephrine impacts activity of cortical astrocytes SP - 51 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2015 ER -
@conference{ author = "Nikolic, Ljiljana and Bataveljic, Danijela and Bijelic, Dunja and Milicevic, Katarina and Bogdanović Pristov, Jelena", year = "2023", abstract = "The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, attention, cognitive function and stress responses. Unlike fast neurotransmitters which act at synapses, NE is released in the neuropil and performs multiple targeting in the surrounding area. The glial cells astrocytes are a direct target of NE, as they express all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial responses elicited by strong and transient increases of NE in the brain are well studied, but the effect of a low background NE concentration on astrocytes is unknown. This background level of NE is maintained by basal noradrenergic activity and is constantly present in the brain. Therefore, the response of astrocytes to the background NE could have been unintentionally evoked in previous studies but its effect overlooked. To assess action of background NE on astrocytes we combined the whole-cell patch clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured cortical astrocytes to bypass NE targeting of multiple cell types. We show that cortical astrocytes detect and respond to the background NE concentration with an increase in intracellular Ca2+. This Ca2+ liberated from intracellular stores further increased large-conductance, Ca2+-sensitive potassium (BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat cortex. Furthermore, stimulation of astrocytes by background NE was inhibited by alpha-adrenoceptor antagonist. Our results suggest that astrocytes maintain basal brain activity by perceiving and responding to the background NE.", publisher = "Serbian Neuroscience Society, Belgrade", journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS", title = "Background norepinephrine impacts activity of cortical astrocytes", pages = "51", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2015" }
Nikolic, L., Bataveljic, D., Bijelic, D., Milicevic, K.,& Bogdanović Pristov, J.. (2023). Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS Serbian Neuroscience Society, Belgrade., 51. https://hdl.handle.net/21.15107/rcub_rimsi_2015
Nikolic L, Bataveljic D, Bijelic D, Milicevic K, Bogdanović Pristov J. Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:51. https://hdl.handle.net/21.15107/rcub_rimsi_2015 .
Nikolic, Ljiljana, Bataveljic, Danijela, Bijelic, Dunja, Milicevic, Katarina, Bogdanović Pristov, Jelena, "Background norepinephrine impacts activity of cortical astrocytes" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):51, https://hdl.handle.net/21.15107/rcub_rimsi_2015 .