TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Djordjevic, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1570
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
SP  - 9023
VL  - 23
DO  - 10.3390/ijms23169023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović Milutinović, Danijela and Blagojević, Duško and Djordjevic, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
pages = "9023",
volume = "23",
doi = "10.3390/ijms23169023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović Milutinović, D., Blagojević, D., Djordjevic, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović Milutinović D, Blagojević D, Djordjevic A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović Milutinović, Danijela, Blagojević, Duško, Djordjevic, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasic, Snežana
AU  - Miletic, Srdjan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1050
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
EP  - 136
SP  - 132
VL  - 100
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasic, Snežana and Miletic, Srdjan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
pages = "136-132",
volume = "100",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasic, S., Miletic, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier Science Bv, Amsterdam., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasic S, Miletic S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasic, Snežana, Miletic, Srdjan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasic, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Djurović, Dijana
AU  - Miletic, Srdjan
AU  - Vrvić, Miroslav M.
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/974
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
EP  - 906
IS  - 6
SP  - 901
VL  - 62
DO  - 10.1097/MPG.0000000000001090
ER  - 

@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasic, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Djurović, Dijana and Miletic, Srdjan and Vrvić, Miroslav M. and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
pages = "906-901",
number = "6",
volume = "62",
doi = "10.1097/MPG.0000000000001090"
}

Marinković, V., Rankovic-Janevski, M., Spasic, S., Nikolić-Kokić, A., Lugonja, N., Djurović, D., Miletic, S., Vrvić, M. M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090

Marinković V, Rankovic-Janevski M, Spasic S, Nikolić-Kokić A, Lugonja N, Djurović D, Miletic S, Vrvić MM, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .

Marinković, Vesna, Rankovic-Janevski, Milica, Spasic, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Djurović, Dijana, Miletic, Srdjan, Vrvić, Miroslav M., Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Andjus, Pavle R.
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/902
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effects of wild-type and mutant sod1 on smooth muscle contraction
EP  - 192
IS  - 1
SP  - 187
VL  - 67
DO  - 10.2298/ABS141006023N
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica D and Andjus, Pavle R. and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effects of wild-type and mutant sod1 on smooth muscle contraction",
pages = "192-187",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z. D., Andjus, P. R.,& Spasić, M.. (2015). The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević ZD, Andjus PR, Spasić M. The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica D, Andjus, Pavle R., Spasić, Mihajlo, "The effects of wild-type and mutant sod1 on smooth muscle contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijusković, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snežana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/846
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
EP  - 23
SP  - 19
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijusković, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snežana and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
pages = "23-19",
volume = "51",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijusković, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijusković A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijusković, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snežana, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Slavić, Marija
AU  - Mijusković, Ana
AU  - Paskulin, Roman
AU  - Miljević, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/889
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes
EP  - 70
SP  - 64
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Slavić, Marija and Mijusković, Ana and Paskulin, Roman and Miljević, Cedo and Spasić, Mihajlo and Blagojević, Duško P.",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes",
pages = "70-64",
volume = "164",
doi = "10.1016/j.jep.2015.01.037"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Slavić, M., Mijusković, A., Paskulin, R., Miljević, C., Spasić, M.,& Blagojević, D. P.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Slavić M, Mijusković A, Paskulin R, Miljević C, Spasić M, Blagojević DP. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Slavić, Marija, Mijusković, Ana, Paskulin, Roman, Miljević, Cedo, Spasić, Mihajlo, Blagojević, Duško P., "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - GEN
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/774
AB  - H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
EP  - S37
SP  - S36
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
ER  - 

@misc{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
pages = "S37-S36",
volume = "39",
doi = "10.1016/j.niox.2014.03.119"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 39, S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39:S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - JOUR
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/787
AB  - Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.
PB  - Polish Acad Sciences Inst Pharmacology, Krakow
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity
EP  - 379
IS  - 3
SP  - 373
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
ER  - 

@article{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.",
publisher = "Polish Acad Sciences Inst Pharmacology, Krakow",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity",
pages = "379-373",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports
Polish Acad Sciences Inst Pharmacology, Krakow., 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Spasić, Mihajlo
AU  - Andjus, Pavle R.
AU  - Savić, Danijela Z
AU  - Parabucki, Ana B
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/707
AB  - The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia
IS  - 10
VL  - 8
DO  - 10.1371/journal.pone.0076383
ER  - 

@article{
author = "Bajić, Aleksandar and Spasić, Mihajlo and Andjus, Pavle R. and Savić, Danijela Z and Parabucki, Ana B and Nikolić-Kokić, Aleksandra and Spasojević, Ivan",
year = "2013",
abstract = "The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia",
number = "10",
volume = "8",
doi = "10.1371/journal.pone.0076383"
}

Bajić, A., Spasić, M., Andjus, P. R., Savić, D. Z., Parabucki, A. B., Nikolić-Kokić, A.,& Spasojević, I.. (2013). Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One
Public Library Science, San Francisco., 8(10).
https://doi.org/10.1371/journal.pone.0076383

Bajić A, Spasić M, Andjus PR, Savić DZ, Parabucki AB, Nikolić-Kokić A, Spasojević I. Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One. 2013;8(10).
doi:10.1371/journal.pone.0076383 .

Bajić, Aleksandar, Spasić, Mihajlo, Andjus, Pavle R., Savić, Danijela Z, Parabucki, Ana B, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia" in PLoS One, 8, no. 10 (2013),
https://doi.org/10.1371/journal.pone.0076383 . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Spasic, Snežana D
AU  - Laugier, Olga B
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Oreščanin-Dušić, Zorana
AU  - Vrvić, Miroslav M
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/700
AB  - Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.
PB  - Elsevier Science Inc, New York
T2  - Nutrition
T1  - Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas
EP  - 435
IS  - 2
SP  - 431
VL  - 29
DO  - 10.1016/j.nut.2012.07.018
ER  - 

@article{
author = "Lugonja, Nikoleta and Spasic, Snežana D and Laugier, Olga B and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Oreščanin-Dušić, Zorana and Vrvić, Miroslav M",
year = "2013",
abstract = "Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.",
publisher = "Elsevier Science Inc, New York",
journal = "Nutrition",
title = "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas",
pages = "435-431",
number = "2",
volume = "29",
doi = "10.1016/j.nut.2012.07.018"
}

Lugonja, N., Spasic, S. D., Laugier, O. B., Nikolić-Kokić, A., Spasojević, I., Oreščanin-Dušić, Z.,& Vrvić, M. M.. (2013). Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition
Elsevier Science Inc, New York., 29(2), 431-435.
https://doi.org/10.1016/j.nut.2012.07.018

Lugonja N, Spasic SD, Laugier OB, Nikolić-Kokić A, Spasojević I, Oreščanin-Dušić Z, Vrvić MM. Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition. 2013;29(2):431-435.
doi:10.1016/j.nut.2012.07.018 .

Lugonja, Nikoleta, Spasic, Snežana D, Laugier, Olga B, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Oreščanin-Dušić, Zorana, Vrvić, Miroslav M, "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas" in Nutrition, 29, no. 2 (2013):431-435,
https://doi.org/10.1016/j.nut.2012.07.018 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Slavić, Marija
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/680
AB  - Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p lt 0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.
AB  - Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
T1  - The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
EP  - 379
IS  - 4
SP  - 375
VL  - 32
DO  - 10.2478/jomb-2013-0032
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Slavić, Marija and Spasojević, Ivan and Stević, Zorica D and Spasić, Mihajlo and Blagojević, Duško P",
year = "2013",
abstract = "Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p lt 0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata., Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića, The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions",
pages = "379-375",
number = "4",
volume = "32",
doi = "10.2478/jomb-2013-0032"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Slavić, M., Spasojević, I., Stević, Z. D., Spasić, M.,& Blagojević, D. P.. (2013). Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(4), 375-379.
https://doi.org/10.2478/jomb-2013-0032

Nikolić-Kokić A, Oreščanin-Dušić Z, Slavić M, Spasojević I, Stević ZD, Spasić M, Blagojević DP. Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry. 2013;32(4):375-379.
doi:10.2478/jomb-2013-0032 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Slavić, Marija, Spasojević, Ivan, Stević, Zorica D, Spasić, Mihajlo, Blagojević, Duško P, "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića" in Journal of Medical Biochemistry, 32, no. 4 (2013):375-379,
https://doi.org/10.2478/jomb-2013-0032 . .

TY  - JOUR
AU  - Ignjatović, Aleksandar
AU  - Stević, Zorica D
AU  - Lavrnic, Dragana S
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/535
AB  - ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.
PB  - Informa Healthcare, London
T2  - Amyotrophic Lateral Sclerosis
T1  - Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 362
IS  - 4
SP  - 357
VL  - 13
DO  - 10.3109/17482968.2012.665929
ER  - 

@article{
author = "Ignjatović, Aleksandar and Stević, Zorica D and Lavrnic, Dragana S and Nikolić-Kokić, Aleksandra and Blagojević, Duško P and Spasić, Mihajlo and Spasojević, Ivan",
year = "2012",
abstract = "ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.",
publisher = "Informa Healthcare, London",
journal = "Amyotrophic Lateral Sclerosis",
title = "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "362-357",
number = "4",
volume = "13",
doi = "10.3109/17482968.2012.665929"
}

Ignjatović, A., Stević, Z. D., Lavrnic, D. S., Nikolić-Kokić, A., Blagojević, D. P., Spasić, M.,& Spasojević, I.. (2012). Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis
Informa Healthcare, London., 13(4), 357-362.
https://doi.org/10.3109/17482968.2012.665929

Ignjatović A, Stević ZD, Lavrnic DS, Nikolić-Kokić A, Blagojević DP, Spasić M, Spasojević I. Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis. 2012;13(4):357-362.
doi:10.3109/17482968.2012.665929 .

Ignjatović, Aleksandar, Stević, Zorica D, Lavrnic, Dragana S, Nikolić-Kokić, Aleksandra, Blagojević, Duško P, Spasić, Mihajlo, Spasojević, Ivan, "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Amyotrophic Lateral Sclerosis, 13, no. 4 (2012):357-362,
https://doi.org/10.3109/17482968.2012.665929 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/410
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 86
IS  - 2
SP  - 81
VL  - 15
DO  - 10.1179/174329210X12650506623320
ER  - 

@article{
author = "Spasojević, Ivan and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško P and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "86-81",
number = "2",
volume = "15",
doi = "10.1179/174329210X12650506623320"
}

Spasojević, I., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D. P.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Taylor & Francis Ltd, Abingdon., 15(2), 81-86.
https://doi.org/10.1179/174329210X12650506623320

Spasojević I, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević DP, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):81-86.
doi:10.1179/174329210X12650506623320 .

Spasojević, Ivan, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško P, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010):81-86,
https://doi.org/10.1179/174329210X12650506623320 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasic, Snežana D.
AU  - Jones, David R
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/321
AB  - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
EP  - 84
IS  - 1
SP  - 80
VL  - 344
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
pages = "84-80",
number = "1",
volume = "344",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Oreščanin-Dušić, Zorana
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojicic, Ratko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/335
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
EP  - 54
IS  - 2
SP  - 48
VL  - 14
DO  - 10.1179/135100009X392476
ER  - 

@article{
author = "Oreščanin-Dušić, Zorana and Milovanović, Slobodan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojicic, Ratko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
pages = "54-48",
number = "2",
volume = "14",
doi = "10.1179/135100009X392476"
}

Oreščanin-Dušić, Z., Milovanović, S., Blagojević, D., Nikolić-Kokić, A., Radojicic, R., Spasojević, I.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report
Taylor & Francis Ltd, Abingdon., 14(2), 48-54.
https://doi.org/10.1179/135100009X392476

Oreščanin-Dušić Z, Milovanović S, Blagojević D, Nikolić-Kokić A, Radojicic R, Spasojević I, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):48-54.
doi:10.1179/135100009X392476 .

Oreščanin-Dušić, Zorana, Milovanović, Slobodan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojicic, Ratko, Spasojević, Ivan, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009):48-54,
https://doi.org/10.1179/135100009X392476 . .