TY  - JOUR
AU  - Savic, Jasna
AU  - Pavlović, Jelena
AU  - Stanojević, Miloš
AU  - Bosnić, Predrag
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1911
AB  - Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.
PB  - MDPI
T2  - Plants
T1  - Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves
SP  - 1660
VL  - 12
DO  - 10.3390/ plants12081660
ER  - 

@article{
author = "Savic, Jasna and Pavlović, Jelena and Stanojević, Miloš and Bosnić, Predrag and Kostić Kravljanac, Ljiljana and Nikolić, Nina and Nikolic, Miroslav",
year = "2023",
abstract = "Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.",
publisher = "MDPI",
journal = "Plants",
title = "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves",
pages = "1660",
volume = "12",
doi = "10.3390/ plants12081660"
}

Savic, J., Pavlović, J., Stanojević, M., Bosnić, P., Kostić Kravljanac, L., Nikolić, N.,& Nikolic, M.. (2023). Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants
MDPI., 12, 1660.
https://doi.org/10.3390/ plants12081660

Savic J, Pavlović J, Stanojević M, Bosnić P, Kostić Kravljanac L, Nikolić N, Nikolic M. Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants. 2023;12:1660.
doi:10.3390/ plants12081660 .

Savic, Jasna, Pavlović, Jelena, Stanojević, Miloš, Bosnić, Predrag, Kostić Kravljanac, Ljiljana, Nikolić, Nina, Nikolic, Miroslav, "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves" in Plants, 12 (2023):1660,
https://doi.org/10.3390/ plants12081660 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Nikolić, Nina
AU  - Milanovic, Slobodan
AU  - Milenkovic, Ivan
AU  - Pavlović, Jelena
AU  - Paravinja, Ana
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2101
AB  - Beneficial effects of silicon (Si) on plants have primarily been studied in crop species under single stress. Moreover, nutrient acquisition-based responses to combination of biotic and abiotic stresses (a common situation in natural habitats) have rarely been reported, in particular in conjunction with soil amendments with Si. Pedunculate oak (Quercus robur L.), one of the ecologically and economically most important tree species in Europe, is facing a severe decline due to combined stresses, but also problems in assisted regeneration in nurseries. Here, we studied the effect of Si supply on the leaf nutriome, root traits and overall growth of 12-weeks-old oak seedlings exposed to abiotic stress [low phosphorus (P) supply], biotic stress (Phytophthora plurivora root infection), and their combination. The application of Si had the strongest ameliorative effect on growth, root health and root phenome under the most severe stress conditions (i.e., combination of P deficiency and P. plurivora root infection), where it differentially affected the uptake and leaf accumulation in 11 out of 13 analysed nutrients. Silicon supply tended to reverse the pattern of change of some, but not all, leaf nutrients affected by stresses: P, boron (B) and magnesium (Mg) under P deficiency, and P, B and sulphur (S) under pathogen attack, but also nickel (Ni) and molybdenum (Mo) under all three stresses. Surprisingly, Si affected some nutrients that were not changed by a particular stress itself and decreased leaf Mg levels under all the stresses. On the other hand, pathogen attack increased leaf accumulation of Si. This exploratory work presents the complexity of nutrient crosstalk under three stresses, and opens more questions about genetic networks that control plant physiological responses. Practically, we show a potential of Si application to improve P status and root health in oak seedlings, particularly in nurseries.
PB  - Frontiers
T2  - Frontiers in Plant Science
T1  - Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection
IS  - 14
SP  - 1265782.
DO  - 10.3389/fpls.2023.1265782
ER  - 

@article{
author = "Kostić, Igor and Nikolić, Nina and Milanovic, Slobodan and Milenkovic, Ivan and Pavlović, Jelena and Paravinja, Ana and Nikolic, Miroslav",
year = "2023",
abstract = "Beneficial effects of silicon (Si) on plants have primarily been studied in crop species under single stress. Moreover, nutrient acquisition-based responses to combination of biotic and abiotic stresses (a common situation in natural habitats) have rarely been reported, in particular in conjunction with soil amendments with Si. Pedunculate oak (Quercus robur L.), one of the ecologically and economically most important tree species in Europe, is facing a severe decline due to combined stresses, but also problems in assisted regeneration in nurseries. Here, we studied the effect of Si supply on the leaf nutriome, root traits and overall growth of 12-weeks-old oak seedlings exposed to abiotic stress [low phosphorus (P) supply], biotic stress (Phytophthora plurivora root infection), and their combination. The application of Si had the strongest ameliorative effect on growth, root health and root phenome under the most severe stress conditions (i.e., combination of P deficiency and P. plurivora root infection), where it differentially affected the uptake and leaf accumulation in 11 out of 13 analysed nutrients. Silicon supply tended to reverse the pattern of change of some, but not all, leaf nutrients affected by stresses: P, boron (B) and magnesium (Mg) under P deficiency, and P, B and sulphur (S) under pathogen attack, but also nickel (Ni) and molybdenum (Mo) under all three stresses. Surprisingly, Si affected some nutrients that were not changed by a particular stress itself and decreased leaf Mg levels under all the stresses. On the other hand, pathogen attack increased leaf accumulation of Si. This exploratory work presents the complexity of nutrient crosstalk under three stresses, and opens more questions about genetic networks that control plant physiological responses. Practically, we show a potential of Si application to improve P status and root health in oak seedlings, particularly in nurseries.",
publisher = "Frontiers",
journal = "Frontiers in Plant Science",
title = "Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection",
number = "14",
pages = "1265782.",
doi = "10.3389/fpls.2023.1265782"
}

Kostić, I., Nikolić, N., Milanovic, S., Milenkovic, I., Pavlović, J., Paravinja, A.,& Nikolic, M.. (2023). Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection. in Frontiers in Plant Science
Frontiers.(14), 1265782..
https://doi.org/10.3389/fpls.2023.1265782

Kostić I, Nikolić N, Milanovic S, Milenkovic I, Pavlović J, Paravinja A, Nikolic M. Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection. in Frontiers in Plant Science. 2023;(14):1265782..
doi:10.3389/fpls.2023.1265782 .

Kostić, Igor, Nikolić, Nina, Milanovic, Slobodan, Milenkovic, Ivan, Pavlović, Jelena, Paravinja, Ana, Nikolic, Miroslav, "Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection" in Frontiers in Plant Science, no. 14 (2023):1265782.,
https://doi.org/10.3389/fpls.2023.1265782 . .

TY  - JOUR
AU  - Hajiboland, Roghieh
AU  - Moradi, Aiuob
AU  - Kahneh, Ehsan
AU  - Poschenrieder, Charlotte
AU  - Nazari, Fatemeh
AU  - Pavlović, Jelena
AU  - Tolra, Roser
AU  - Salehi-Lisar, Seyed-Yahya
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1979
AB  - Increased availability of toxic Al3+ is the main constraint limiting plant growth on acid soils. Plants adapted to acid soils, however, tolerate toxic Al3+, and some can accumulate Al in their aerial parts to a significant degree. Studies on Al-tolerant and Al-accumulating species have mainly focused on the vegetation of acid soils distributed as two global belts in the northern and southern hemispheres, while acid soils formed outside these regions have been largely neglected. The acid soils (pH 3.4–4.2) of the tea plantations in the south Caspian region of Northern Iran were surveyed over three seasons at two main locations. Aluminum and other mineral elements (including nutrients) were measured in 499 plant specimens representing 86 species from 43 families. Al accumulation exceeding the criterion for accumulator species (>1000 μg g−1 DW) was found in 36 species belonging to 23 families of herbaceous annual or perennial angiosperms, in addition to three bryophyte species. Besides Al, Fe accumulation (1026–5155 μg g−1 DW) was also observed in the accumulator species that exceeded the critical toxicity concentration, whereas no such accumulation was observed for Mn. The majority of analyzed accumulator plants (64%) were cosmopolitan or pluriregional species, with a considerable rate of Euro-Siberian elements (37%). Our findings, which may contribute to phylogenetic studies of Al accumulators, also suggest suitable accumulator and excluder species for the rehabilitation of acid-eroded soils and introduce new model species for investigating Al accumulation and exclusion mechanisms.
PB  - MDPI, Basel, Switzerland
T2  - Plants
T1  - Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators
SP  - 2129
VL  - 12
DO  - doi.org/10.3390/plants12112129
ER  - 

@article{
author = "Hajiboland, Roghieh and Moradi, Aiuob and Kahneh, Ehsan and Poschenrieder, Charlotte and Nazari, Fatemeh and Pavlović, Jelena and Tolra, Roser and Salehi-Lisar, Seyed-Yahya and Nikolic, Miroslav",
year = "2023",
abstract = "Increased availability of toxic Al3+ is the main constraint limiting plant growth on acid soils. Plants adapted to acid soils, however, tolerate toxic Al3+, and some can accumulate Al in their aerial parts to a significant degree. Studies on Al-tolerant and Al-accumulating species have mainly focused on the vegetation of acid soils distributed as two global belts in the northern and southern hemispheres, while acid soils formed outside these regions have been largely neglected. The acid soils (pH 3.4–4.2) of the tea plantations in the south Caspian region of Northern Iran were surveyed over three seasons at two main locations. Aluminum and other mineral elements (including nutrients) were measured in 499 plant specimens representing 86 species from 43 families. Al accumulation exceeding the criterion for accumulator species (>1000 μg g−1 DW) was found in 36 species belonging to 23 families of herbaceous annual or perennial angiosperms, in addition to three bryophyte species. Besides Al, Fe accumulation (1026–5155 μg g−1 DW) was also observed in the accumulator species that exceeded the critical toxicity concentration, whereas no such accumulation was observed for Mn. The majority of analyzed accumulator plants (64%) were cosmopolitan or pluriregional species, with a considerable rate of Euro-Siberian elements (37%). Our findings, which may contribute to phylogenetic studies of Al accumulators, also suggest suitable accumulator and excluder species for the rehabilitation of acid-eroded soils and introduce new model species for investigating Al accumulation and exclusion mechanisms.",
publisher = "MDPI, Basel, Switzerland",
journal = "Plants",
title = "Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators",
pages = "2129",
volume = "12",
doi = "doi.org/10.3390/plants12112129"
}

Hajiboland, R., Moradi, A., Kahneh, E., Poschenrieder, C., Nazari, F., Pavlović, J., Tolra, R., Salehi-Lisar, S.,& Nikolic, M.. (2023). Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators. in Plants
MDPI, Basel, Switzerland., 12, 2129.
https://doi.org/doi.org/10.3390/plants12112129

Hajiboland R, Moradi A, Kahneh E, Poschenrieder C, Nazari F, Pavlović J, Tolra R, Salehi-Lisar S, Nikolic M. Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators. in Plants. 2023;12:2129.
doi:doi.org/10.3390/plants12112129 .

Hajiboland, Roghieh, Moradi, Aiuob, Kahneh, Ehsan, Poschenrieder, Charlotte, Nazari, Fatemeh, Pavlović, Jelena, Tolra, Roser, Salehi-Lisar, Seyed-Yahya, Nikolic, Miroslav, "Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators" in Plants, 12 (2023):2129,
https://doi.org/doi.org/10.3390/plants12112129 . .

TY  - CONF
AU  - Trailović, Maja
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - TODIC, Slavica
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2140
AB  - Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2140
ER  - 

@conference{
author = "Trailović, Maja and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Pavlović, Jelena and Bosnić, Predrag and TODIC, Slavica and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2140"
}

Trailović, M., Kostić Kravljanac, L., Stanojević, M., Pavlović, J., Bosnić, P., TODIC, S.,& Nikolic, M.. (2022). Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2140

Trailović M, Kostić Kravljanac L, Stanojević M, Pavlović J, Bosnić P, TODIC S, Nikolic M. Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

Trailović, Maja, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Pavlović, Jelena, Bosnić, Predrag, TODIC, Slavica, Nikolic, Miroslav, "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

TY  - CONF
AU  - Kostić Kravljanac, Ljiljana
AU  - Trailović, Maja
AU  - Pavlović, Jelena
AU  - Kostić, Igor
AU  - Dubljanin, Tijana
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2135
AB  - Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2135
ER  - 

@conference{
author = "Kostić Kravljanac, Ljiljana and Trailović, Maja and Pavlović, Jelena and Kostić, Igor and Dubljanin, Tijana and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2135"
}

Kostić Kravljanac, L., Trailović, M., Pavlović, J., Kostić, I., Dubljanin, T.,& Nikolic, M.. (2022). Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2135

Kostić Kravljanac L, Trailović M, Pavlović J, Kostić I, Dubljanin T, Nikolic M. Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

Kostić Kravljanac, Ljiljana, Trailović, Maja, Pavlović, Jelena, Kostić, Igor, Dubljanin, Tijana, Nikolic, Miroslav, "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Hernandez-Apaolaza, Lourdes
AU  - Dubljanin, Tijana
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2134
AB  - Zinc (Zn), an essential micronutrient for crops, is involved in a variety of physiological processes. The Zn deficiency mainly affects generative growth and seed development and being a component of the antioxidant machinery (e.g., Cu/Zn-SOD) is conductive to oxidative stress in plant tissues. Rice is a typical silicon (Si)-accumulating species, which is strongly affected by Zn deficiency in the alkaline, low Zn soils, especially high in phosphate and/or organic matter. Yet, little is known about the interaction between Si and Zn in rice plants under Zn-deficient conditions. We investigated the effect of Si nutrition on Zn tissue distribution and biosynthesis of organic acid in rice plants subjected to short-term (up to 7 days) and long-term (28 days) Zn deficiency. Tissue concentrations of organic acids by HPLC in parallel Zn and Si by ICP-OES were measured. The Si addition to the nutrient solution successfully mitigated visual symptoms of Zn-deficiency stress and significantly increased dry biomass of rice plants. Interestingly, during the short-term experiment, +Zn plants supplied with Si showed significantly lower Zn concentration in the shoots, but significantly higher Zn concentration in the roots. Also, in the -Zn plants supplied with Si, the concentration of Zn in root tissue rapidly decreased to the level of -Si/-Zn plants, which was followed by an increased concentrations of both organic acids and Zn in the shoots. In the long-term experiment, however, Si did not affect Zn concentration in roots nor in shoots of -Zn plants, but Si differently affected organic acid profile and their tissue accumulation depending on the plant organ and Zn status. In conclusion, Si supply enhanced root-to-shoot translocation of Zn mediated by organic acid ligands during the first 7 days of Zn deficiency.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Silicon Enhances the Biosynthesis of Organic Acids in Zinc-deficient Rice
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2134
ER  - 

@conference{
author = "Pavlović, Jelena and Hernandez-Apaolaza, Lourdes and Dubljanin, Tijana and Nikolic, Miroslav",
year = "2022",
abstract = "Zinc (Zn), an essential micronutrient for crops, is involved in a variety of physiological processes. The Zn deficiency mainly affects generative growth and seed development and being a component of the antioxidant machinery (e.g., Cu/Zn-SOD) is conductive to oxidative stress in plant tissues. Rice is a typical silicon (Si)-accumulating species, which is strongly affected by Zn deficiency in the alkaline, low Zn soils, especially high in phosphate and/or organic matter. Yet, little is known about the interaction between Si and Zn in rice plants under Zn-deficient conditions. We investigated the effect of Si nutrition on Zn tissue distribution and biosynthesis of organic acid in rice plants subjected to short-term (up to 7 days) and long-term (28 days) Zn deficiency. Tissue concentrations of organic acids by HPLC in parallel Zn and Si by ICP-OES were measured. The Si addition to the nutrient solution successfully mitigated visual symptoms of Zn-deficiency stress and significantly increased dry biomass of rice plants. Interestingly, during the short-term experiment, +Zn plants supplied with Si showed significantly lower Zn concentration in the shoots, but significantly higher Zn concentration in the roots. Also, in the -Zn plants supplied with Si, the concentration of Zn in root tissue rapidly decreased to the level of -Si/-Zn plants, which was followed by an increased concentrations of both organic acids and Zn in the shoots. In the long-term experiment, however, Si did not affect Zn concentration in roots nor in shoots of -Zn plants, but Si differently affected organic acid profile and their tissue accumulation depending on the plant organ and Zn status. In conclusion, Si supply enhanced root-to-shoot translocation of Zn mediated by organic acid ligands during the first 7 days of Zn deficiency.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Silicon Enhances the Biosynthesis of Organic Acids in Zinc-deficient Rice",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2134"
}

Pavlović, J., Hernandez-Apaolaza, L., Dubljanin, T.,& Nikolic, M.. (2022). Silicon Enhances the Biosynthesis of Organic Acids in Zinc-deficient Rice. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2134

Pavlović J, Hernandez-Apaolaza L, Dubljanin T, Nikolic M. Silicon Enhances the Biosynthesis of Organic Acids in Zinc-deficient Rice. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2134 .

Pavlović, Jelena, Hernandez-Apaolaza, Lourdes, Dubljanin, Tijana, Nikolic, Miroslav, "Silicon Enhances the Biosynthesis of Organic Acids in Zinc-deficient Rice" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2134 .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Kirkby, Ernest A.
AU  - Nikolic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1425
AB  - Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Interactions of Silicon With Essential and Beneficial Elements in Plants
VL  - 12
DO  - 10.3389/fpls.2021.697592
ER  - 

@article{
author = "Pavlović, Jelena and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Kirkby, Ernest A. and Nikolic, Miroslav",
year = "2021",
abstract = "Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Interactions of Silicon With Essential and Beneficial Elements in Plants",
volume = "12",
doi = "10.3389/fpls.2021.697592"
}

Pavlović, J., Kostić Kravljanac, L., Bosnić, P., Kirkby, E. A.,& Nikolic, M.. (2021). Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fpls.2021.697592

Pavlović J, Kostić Kravljanac L, Bosnić P, Kirkby EA, Nikolic M. Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science. 2021;12.
doi:10.3389/fpls.2021.697592 .

Pavlović, Jelena, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Kirkby, Ernest A., Nikolic, Miroslav, "Interactions of Silicon With Essential and Beneficial Elements in Plants" in Frontiers in Plant Science, 12 (2021),
https://doi.org/10.3389/fpls.2021.697592 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Nikolic, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1212
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnic, Dragana and Nikolic, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnic, D., Nikolic, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J. T.,& Nikolic, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnic D, Nikolic D, Timotijević G, Pavlović J, Vaculik M, Samardžić JT, Nikolic M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnic, Dragana, Nikolic, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2139
AB  - Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in
the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants. Due to poor Fe availability for higher plants, especially in alkaline and calcareous soils, Fe deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality, with a strong negative impact on human health.
Here we investigated the key physiological, biochemical and molecular parameters involved in
the processes of root acquisition and tissue utilization of Fe by cucumber (Cucumis sativus L.), as
both Strategy 1 model and Si-accumulating species.
Silicon nutrition increased the accumulation of apoplastic Fe and Fe-mobilizing compounds in
roots, as well as upregulated the expression of genes (AHA1, FRO2, IRT1) encoding the main components
of the reduction-based Fe uptake machinery (Pavlovic et al., 2013). In leaves, Si affected
relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves (Pavlovic et al., 2016). This for the first time demonstrated a new beneficial role of Si, i.e. in increasing nutrient acquisition, transport and utilization by crops.
References:
Pavlovic J., Samardzic J., Kostic L., Laursen K.H., Natic M., Timotijevic G., Schjoerring J.K., Nikolic M. (2016): Ann. Bot. 118, 271-280.
Pavlovic J., Samardzic J., Maksimović V., Timotijevic G., Stevic N., Laursen K.H., Hansen T.H., Husted S., Schjoerring J.K., Liang Y., Nikolic M. (2013): New Phytol. 198, 1096-1107.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), June 9-12, 2018, Belgrade, Serbia
T1  - Silicon increases iron use efficiency in cucumber – a strategy 1 model plant
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2139
ER  - 

@conference{
author = "Pavlović, Jelena and Nikolic, Miroslav",
year = "2018",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in
the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants. Due to poor Fe availability for higher plants, especially in alkaline and calcareous soils, Fe deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality, with a strong negative impact on human health.
Here we investigated the key physiological, biochemical and molecular parameters involved in
the processes of root acquisition and tissue utilization of Fe by cucumber (Cucumis sativus L.), as
both Strategy 1 model and Si-accumulating species.
Silicon nutrition increased the accumulation of apoplastic Fe and Fe-mobilizing compounds in
roots, as well as upregulated the expression of genes (AHA1, FRO2, IRT1) encoding the main components
of the reduction-based Fe uptake machinery (Pavlovic et al., 2013). In leaves, Si affected
relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves (Pavlovic et al., 2016). This for the first time demonstrated a new beneficial role of Si, i.e. in increasing nutrient acquisition, transport and utilization by crops.
References:
Pavlovic J., Samardzic J., Kostic L., Laursen K.H., Natic M., Timotijevic G., Schjoerring J.K., Nikolic M. (2016): Ann. Bot. 118, 271-280.
Pavlovic J., Samardzic J., Maksimović V., Timotijevic G., Stevic N., Laursen K.H., Hansen T.H., Husted S., Schjoerring J.K., Liang Y., Nikolic M. (2013): New Phytol. 198, 1096-1107.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), June 9-12, 2018, Belgrade, Serbia",
title = "Silicon increases iron use efficiency in cucumber – a strategy 1 model plant",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2139"
}

Pavlović, J.,& Nikolic, M.. (2018). Silicon increases iron use efficiency in cucumber – a strategy 1 model plant. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), June 9-12, 2018, Belgrade, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2139

Pavlović J, Nikolic M. Silicon increases iron use efficiency in cucumber – a strategy 1 model plant. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), June 9-12, 2018, Belgrade, Serbia. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2139 .

Pavlović, Jelena, Nikolic, Miroslav, "Silicon increases iron use efficiency in cucumber – a strategy 1 model plant" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), June 9-12, 2018, Belgrade, Serbia (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2139 .

TY  - CHAP
AU  - Nikolic, Miroslav
AU  - Pavlović, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1783
AB  - Iron (Fe) is the fourth most abundant mineral in the Earth's crust essential for plant growth. However, if overloaded, Fe becomes toxic for plants as a highly reactive Fenton catalyst. Higher plants have developed two distinct adaptive strategies to cope with low Fe availability in soils, such as the reduction-based strategy (Strategy 1) in nongraminaceous plants, and the chelation-based strategy (Strategy 2) in graminaceous species. The ability of plants to improve Fe availability in the rhizosphere and its internal use efficiency will strongly affect both crop yield and quality in terms of Fe source for humans. Understanding the mechanisms involved in Fe uptake, transport, and storage is essential for breeding crops more tolerant to Fe-limited conditions. This review summarizes the current knowledge of root acquisition of Fe (deficiency), binding and detoxification of Fe (toxicity), long-distance root-to-shoot transport including loading of Fe into edible tissues, and molecular regulation of Fe use efficiency.
PB  - Academic Press, Elsevier
T2  - Plant micronutrient use efficiency
T1  - Plant responses to iron deficiency and toxicity and iron use efficiency in plants
EP  - 69
SP  - 55
DO  - https://doi.org/10.1016/B978-0-12-812104-7.00004-6
ER  - 

@inbook{
author = "Nikolic, Miroslav and Pavlović, Jelena",
year = "2018",
abstract = "Iron (Fe) is the fourth most abundant mineral in the Earth's crust essential for plant growth. However, if overloaded, Fe becomes toxic for plants as a highly reactive Fenton catalyst. Higher plants have developed two distinct adaptive strategies to cope with low Fe availability in soils, such as the reduction-based strategy (Strategy 1) in nongraminaceous plants, and the chelation-based strategy (Strategy 2) in graminaceous species. The ability of plants to improve Fe availability in the rhizosphere and its internal use efficiency will strongly affect both crop yield and quality in terms of Fe source for humans. Understanding the mechanisms involved in Fe uptake, transport, and storage is essential for breeding crops more tolerant to Fe-limited conditions. This review summarizes the current knowledge of root acquisition of Fe (deficiency), binding and detoxification of Fe (toxicity), long-distance root-to-shoot transport including loading of Fe into edible tissues, and molecular regulation of Fe use efficiency.",
publisher = "Academic Press, Elsevier",
journal = "Plant micronutrient use efficiency",
booktitle = "Plant responses to iron deficiency and toxicity and iron use efficiency in plants",
pages = "69-55",
doi = "https://doi.org/10.1016/B978-0-12-812104-7.00004-6"
}

Nikolic, M.,& Pavlović, J.. (2018). Plant responses to iron deficiency and toxicity and iron use efficiency in plants. in Plant micronutrient use efficiency
Academic Press, Elsevier., 55-69.
https://doi.org/https://doi.org/10.1016/B978-0-12-812104-7.00004-6

Nikolic M, Pavlović J. Plant responses to iron deficiency and toxicity and iron use efficiency in plants. in Plant micronutrient use efficiency. 2018;:55-69.
doi:https://doi.org/10.1016/B978-0-12-812104-7.00004-6 .

Nikolic, Miroslav, Pavlović, Jelena, "Plant responses to iron deficiency and toxicity and iron use efficiency in plants" in Plant micronutrient use efficiency (2018):55-69,
https://doi.org/https://doi.org/10.1016/B978-0-12-812104-7.00004-6 . .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2149
AB  - Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.
PB  - University of Copenhagen, Denmark
C3  - XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
T1  - Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2149
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.",
publisher = "University of Copenhagen, Denmark",
journal = "XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark",
title = "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2149"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2149

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress" in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2141
AB  - The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.
PB  - University of Agricultural Sciences, GKVK, Bengaluru
C3  - 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
T1  - Silicon influence on plant ionome and mineral element transporters
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2141
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.",
publisher = "University of Agricultural Sciences, GKVK, Bengaluru",
journal = "7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India",
title = "Silicon influence on plant ionome and mineral element transporters",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2141"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
University of Agricultural Sciences, GKVK, Bengaluru..
https://hdl.handle.net/21.15107/rcub_rimsi_2141

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon influence on plant ionome and mineral element transporters" in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

TY  - THES
AU  - Pavlović, Jelena
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5140
UR  - https://nardus.mpn.gov.rs/handle/123456789/8430
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:15995/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49212431
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/16
AB  - Nedostatak gvožđa (Fe) predstavlja jedan od glavnih ograničavajućih faktora u proizvodnji useva širom sveta, koji značajno umanjuje prinos i kvalitet. Sa druge strane, koristan efekat silicijuma (Si) na rastenje i razviće biljaka, posebno u uslovima stresa, dobro je poznat u literaturi. Cilj ove teze je bio da se prouči mehanizam kako Si ublažava stres izazvan nedostatkom Fe kod dikotiledonih biljaka (strategija I usvajanja Fe), o kome se do sada ništa nije znalo. Kao model biljka odabran je krastavac (Cucumis sativus L.) kao vrsta koja akumulira Si. Izvršena su detaljna izučavanja mobilnosti Fe u apoplastu, biosinteze jedinjenja koja mobilišu Fe i njegovog daljeg usvajanja na bazi redukcije u korenu, zajedno sa ekspresijom gena uključenih u te procese. U nadzemnom delu biljke, proučavana je remobilizacija Fe iz starijih u mlađe listove, tako što su paralelno mereni distribucija 59Fe u listovima na različitim pozicijama i koncentracija helatora nikocijanamina (NA), zajedno sa ekspresijom gena za nikocijanamin sintazu (NAS) uključenu u njegovu biosintezu i YSL (eng. yellow stripe-like) transporter koji posreduju u transportu Fe-NA floemom. Ishrana Si uticala je na povećanu akumulaciju Fe u apoplastu i Fe-mobilizirajućih jedinjenja u korenu, kao i na stimulaciju mašinerije za usvajanje Fe. U listovima, Si je uticao na relativnu distribuciju Fe povećavajući remobilizaciju Fe iz starijih listova zbog povećane akumulacije NA i ekspresije YSL1, što je pospešilo heliranje Fe i njegovu retranslokaciju u mlađe listove. Ova teza pruža, po prvi put, objašnjenje mehanizma koji Si ispoljava u prevazilaženju nedostatka Fe, a koji se sastoji u povećanju rezervi Fe u apoplastu korena, njegove mobilizacije i trasporta iz korenu u izdanak, kao i poboljšane remobilizacije iz starijih u mlađe listove.
AB  - Iron (Fe) deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality. Beneficial effect of silicon (Si) on plant growth and development, especially under stress conditions, is well documented in the literature. The objective of this thesis was to investigate the mechanisms of how Si ameliorates Fe deficiency in Strategy I plants, which has not been understood until recently. Cucumber (Cucumis sativus L.) was selected as a common model plant for Fe research and which is also known as a Si-accumulating dicot. Detailed analyzes of the dynamics of apoplastic Fe, biosynthesis of Fe-mobilizing compounds and reduction-based Fe acquisition were performed in roots along with the expression of relevant genes involved in these processes. At the shoot level, distribution of 59Fe was measured in the in leaves at different positions and developmental stages in parallel with the concentration of the Fe chelator nicotianamine (NA) and the gene expression of nicotianamine synthase (NAS) involved in its biosynthesis. The expression of yellow stripe-like (YSL) transporters mediating pholem transport of Fe-NA in shoot was also determined. Silicon nutrition increased the accumulation of apoplastic Fe and Fe mobilizing compounds in roots. In leaves, Si affected relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves. This thesis for the first time elucidates the mechanism of Si-mediated alleviation of Fe deficiency stress, by increasing the root apoplastic Fe pool, enhancing its acquisition by roots and translocation to shoots and also remobilization of Fe from older to younger leaves.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.)
T1  - The Role of Silicon in Alleviation of Iron Deficiency in Cucumber (Cucumis sativus L.)
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8430
ER  - 

@phdthesis{
author = "Pavlović, Jelena",
year = "2017",
abstract = "Nedostatak gvožđa (Fe) predstavlja jedan od glavnih ograničavajućih faktora u proizvodnji useva širom sveta, koji značajno umanjuje prinos i kvalitet. Sa druge strane, koristan efekat silicijuma (Si) na rastenje i razviće biljaka, posebno u uslovima stresa, dobro je poznat u literaturi. Cilj ove teze je bio da se prouči mehanizam kako Si ublažava stres izazvan nedostatkom Fe kod dikotiledonih biljaka (strategija I usvajanja Fe), o kome se do sada ništa nije znalo. Kao model biljka odabran je krastavac (Cucumis sativus L.) kao vrsta koja akumulira Si. Izvršena su detaljna izučavanja mobilnosti Fe u apoplastu, biosinteze jedinjenja koja mobilišu Fe i njegovog daljeg usvajanja na bazi redukcije u korenu, zajedno sa ekspresijom gena uključenih u te procese. U nadzemnom delu biljke, proučavana je remobilizacija Fe iz starijih u mlađe listove, tako što su paralelno mereni distribucija 59Fe u listovima na različitim pozicijama i koncentracija helatora nikocijanamina (NA), zajedno sa ekspresijom gena za nikocijanamin sintazu (NAS) uključenu u njegovu biosintezu i YSL (eng. yellow stripe-like) transporter koji posreduju u transportu Fe-NA floemom. Ishrana Si uticala je na povećanu akumulaciju Fe u apoplastu i Fe-mobilizirajućih jedinjenja u korenu, kao i na stimulaciju mašinerije za usvajanje Fe. U listovima, Si je uticao na relativnu distribuciju Fe povećavajući remobilizaciju Fe iz starijih listova zbog povećane akumulacije NA i ekspresije YSL1, što je pospešilo heliranje Fe i njegovu retranslokaciju u mlađe listove. Ova teza pruža, po prvi put, objašnjenje mehanizma koji Si ispoljava u prevazilaženju nedostatka Fe, a koji se sastoji u povećanju rezervi Fe u apoplastu korena, njegove mobilizacije i trasporta iz korenu u izdanak, kao i poboljšane remobilizacije iz starijih u mlađe listove., Iron (Fe) deficiency represents a major limiting factor for crop production worldwide, affecting both crop yield and quality. Beneficial effect of silicon (Si) on plant growth and development, especially under stress conditions, is well documented in the literature. The objective of this thesis was to investigate the mechanisms of how Si ameliorates Fe deficiency in Strategy I plants, which has not been understood until recently. Cucumber (Cucumis sativus L.) was selected as a common model plant for Fe research and which is also known as a Si-accumulating dicot. Detailed analyzes of the dynamics of apoplastic Fe, biosynthesis of Fe-mobilizing compounds and reduction-based Fe acquisition were performed in roots along with the expression of relevant genes involved in these processes. At the shoot level, distribution of 59Fe was measured in the in leaves at different positions and developmental stages in parallel with the concentration of the Fe chelator nicotianamine (NA) and the gene expression of nicotianamine synthase (NAS) involved in its biosynthesis. The expression of yellow stripe-like (YSL) transporters mediating pholem transport of Fe-NA in shoot was also determined. Silicon nutrition increased the accumulation of apoplastic Fe and Fe mobilizing compounds in roots. In leaves, Si affected relative Fe distribution by enhancing Fe remobilization from old leaves via increased NA accumulation and expression of the YSL1, which stimulated Fe chelation and its retranslocation to younger leaves. This thesis for the first time elucidates the mechanism of Si-mediated alleviation of Fe deficiency stress, by increasing the root apoplastic Fe pool, enhancing its acquisition by roots and translocation to shoots and also remobilization of Fe from older to younger leaves.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.), The Role of Silicon in Alleviation of Iron Deficiency in Cucumber (Cucumis sativus L.)",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8430"
}

Pavlović, J.. (2017). Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.). 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8430

Pavlović J. Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.). 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8430 .

Pavlović, Jelena, "Uloga silicijuma u prevazilaženju nedostatka gvožđa kod krastavca (Cucumis sativus L.)" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8430 .

TY  - JOUR
AU  - Nikolic, Miroslav
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - Stević, Nenad
AU  - Savić, Jasna
AU  - Hristov, Nikola
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/949
AB  - The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition
EP  - 148
SP  - 141
VL  - 553
DO  - 10.1016/j.scitotenv.2016.02.102
ER  - 

@article{
author = "Nikolic, Miroslav and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag and Stević, Nenad and Savić, Jasna and Hristov, Nikola",
year = "2016",
abstract = "The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition",
pages = "148-141",
volume = "553",
doi = "10.1016/j.scitotenv.2016.02.102"
}

Nikolic, M., Nikolić, N., Kostić Kravljanac, L., Pavlović, J., Bosnić, P., Stević, N., Savić, J.,& Hristov, N.. (2016). The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment
Elsevier, Amsterdam., 553, 141-148.
https://doi.org/10.1016/j.scitotenv.2016.02.102

Nikolic M, Nikolić N, Kostić Kravljanac L, Pavlović J, Bosnić P, Stević N, Savić J, Hristov N. The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment. 2016;553:141-148.
doi:10.1016/j.scitotenv.2016.02.102 .

Nikolic, Miroslav, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, Stević, Nenad, Savić, Jasna, Hristov, Nikola, "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition" in Science of the Total Environment, 553 (2016):141-148,
https://doi.org/10.1016/j.scitotenv.2016.02.102 . .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2455
AB  - Silicon (Si) and iron (Fe) are the second and the fourth most abundant minerals respectively in the earth’s crust. While the essentiality of Fe for plants was discovered in the middle of the 19th century, Si is still not accepted as an essential element. However, its beneficial effect on plant growth and development, especially under stress conditions, is well documented in the literature. As a consequence of poor Fe availability for higher plants, especially in alkaline and calcareous soils, Fe deficiency represents a major nutritional disorder responsible for reduction in both yield and quality of a wide range of crops. Iron deficiency in crops has thus a strong negative impact on human health worldwide. Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe-deficiency. Here we investigated the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe limiting conditions. We determined Fe (57Fe or naturally-occurring isotopes) in leaves at different positions on plants growing with or without Si supply. In parallel the content of the Fe chelator nicotianamine (NA) and the expression of nicotianamine synthase (NAS) involved in its biosynthesis were also measured.
In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. By contrast, Si-treated plants showed an almost even Fe distribution between leaves at four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This stimulated Fe export was paralleled by increased NAS1 gene expression and NA content in expanded leaves of Si-supplied plants. 
In conclusion, our results for the first time show that Si induces Fe mobilization in older leaves and increases its retranslocation to younger expanding leaves. Supply of Si enhances expression of NAS1 gene responsible for NA biosynthesis and hence increased NA accumulation which in turn enhances chelation of Fe and phloem loading of Fe-NA in the source (expanded) leaves to facilitate phloem transport of Fe and enhanced phloem unloading of Fe in the sink (expanding) leaves.
PB  - Department of Agricultural Chemistry and Food Sciences Faculty of Sciences University Autonoma of Madrid
C3  - Abstracts of the 18th International Symposium on Iron Nutrition and Interaction in Plants May 30th – June 3rd, 2016, Madrid, Spain
T1  - SILICON ENHANCES NICOTIANAMINE•MEDIATED IRON TRANSPORT IN CUCUMBER LEAVES
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2455
ER  - 

@conference{
author = "Pavlović, Jelena and Nikolic, Miroslav",
year = "2016",
abstract = "Silicon (Si) and iron (Fe) are the second and the fourth most abundant minerals respectively in the earth’s crust. While the essentiality of Fe for plants was discovered in the middle of the 19th century, Si is still not accepted as an essential element. However, its beneficial effect on plant growth and development, especially under stress conditions, is well documented in the literature. As a consequence of poor Fe availability for higher plants, especially in alkaline and calcareous soils, Fe deficiency represents a major nutritional disorder responsible for reduction in both yield and quality of a wide range of crops. Iron deficiency in crops has thus a strong negative impact on human health worldwide. Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe-deficiency. Here we investigated the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe limiting conditions. We determined Fe (57Fe or naturally-occurring isotopes) in leaves at different positions on plants growing with or without Si supply. In parallel the content of the Fe chelator nicotianamine (NA) and the expression of nicotianamine synthase (NAS) involved in its biosynthesis were also measured.
In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. By contrast, Si-treated plants showed an almost even Fe distribution between leaves at four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This stimulated Fe export was paralleled by increased NAS1 gene expression and NA content in expanded leaves of Si-supplied plants. 
In conclusion, our results for the first time show that Si induces Fe mobilization in older leaves and increases its retranslocation to younger expanding leaves. Supply of Si enhances expression of NAS1 gene responsible for NA biosynthesis and hence increased NA accumulation which in turn enhances chelation of Fe and phloem loading of Fe-NA in the source (expanded) leaves to facilitate phloem transport of Fe and enhanced phloem unloading of Fe in the sink (expanding) leaves.",
publisher = "Department of Agricultural Chemistry and Food Sciences Faculty of Sciences University Autonoma of Madrid",
journal = "Abstracts of the 18th International Symposium on Iron Nutrition and Interaction in Plants May 30th – June 3rd, 2016, Madrid, Spain",
title = "SILICON ENHANCES NICOTIANAMINE•MEDIATED IRON TRANSPORT IN CUCUMBER LEAVES",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2455"
}

Pavlović, J.,& Nikolic, M.. (2016). SILICON ENHANCES NICOTIANAMINE•MEDIATED IRON TRANSPORT IN CUCUMBER LEAVES. in Abstracts of the 18th International Symposium on Iron Nutrition and Interaction in Plants May 30th – June 3rd, 2016, Madrid, Spain
Department of Agricultural Chemistry and Food Sciences Faculty of Sciences University Autonoma of Madrid..
https://hdl.handle.net/21.15107/rcub_rimsi_2455

Pavlović J, Nikolic M. SILICON ENHANCES NICOTIANAMINE•MEDIATED IRON TRANSPORT IN CUCUMBER LEAVES. in Abstracts of the 18th International Symposium on Iron Nutrition and Interaction in Plants May 30th – June 3rd, 2016, Madrid, Spain. 2016;.
https://hdl.handle.net/21.15107/rcub_rimsi_2455 .

Pavlović, Jelena, Nikolic, Miroslav, "SILICON ENHANCES NICOTIANAMINE•MEDIATED IRON TRANSPORT IN CUCUMBER LEAVES" in Abstracts of the 18th International Symposium on Iron Nutrition and Interaction in Plants May 30th – June 3rd, 2016, Madrid, Spain (2016),
https://hdl.handle.net/21.15107/rcub_rimsi_2455 .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Kostić Kravljanac, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natic, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1002
AB  - Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions
EP  - 280
IS  - 2
SP  - 271
VL  - 118
DO  - 10.1093/aob/mcw105
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Kostić Kravljanac, Ljiljana and Laursen, Kristian H. and Natic, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolic, Miroslav",
year = "2016",
abstract = "Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions",
pages = "280-271",
number = "2",
volume = "118",
doi = "10.1093/aob/mcw105"
}

Pavlović, J., Samardžić, J. T., Kostić Kravljanac, L., Laursen, K. H., Natic, M., Timotijević, G., Schjoerring, J. K.,& Nikolic, M.. (2016). Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany
Oxford Univ Press, Oxford., 118(2), 271-280.
https://doi.org/10.1093/aob/mcw105

Pavlović J, Samardžić JT, Kostić Kravljanac L, Laursen KH, Natic M, Timotijević G, Schjoerring JK, Nikolic M. Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany. 2016;118(2):271-280.
doi:10.1093/aob/mcw105 .

Pavlović, Jelena, Samardžić, Jelena T., Kostić Kravljanac, Ljiljana, Laursen, Kristian H., Natic, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolic, Miroslav, "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions" in Annals of Botany, 118, no. 2 (2016):271-280,
https://doi.org/10.1093/aob/mcw105 . .

TY  - JOUR
AU  - Stević, Nenad
AU  - Korać Jačić, Jelena
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1008
AB  - The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).
PB  - Springer, Dordrecht
T2  - Biometals
T1  - Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study
EP  - 951
IS  - 5
SP  - 945
VL  - 29
DO  - 10.1007/s10534-016-9966-9
ER  - 

@article{
author = "Stević, Nenad and Korać Jačić, Jelena and Pavlović, Jelena and Nikolic, Miroslav",
year = "2016",
abstract = "The supplementation of monosilicic acid [Si(OH)(4)] to the root growing medium is known to protect plants from toxic levels of iron (Fe), copper (Cu) and manganese (Mn), but also to mitigate deficiency of Fe and Mn. However, the physicochemical bases of these alleviating mechanisms are not fully understood. Here we applied low-T electron paramagnetic resonance (EPR) spectroscopy to examine the formation of complexes of Si(OH)(4) with Mn2+, Fe3+, and Cu2+ in water and in xylem sap of cucumber (Cucumis sativus L.) grown without or with supply of Si(OH)(4). EPR, which is also useful in establishing the redox state of these metals, was combined with measurements of total concentrations of metals in xylem sap by inductive coupled plasma. Our results show that Si(OH)(4) forms coordination bonds with all three metals. The strongest interactions of Si(OH)(4) appear to be with Cu2+ (1/1 stoichiometry) which might lead to Cu precipitation. In line with this in vitro findings, Si(OH)(4) supply to cucumber resulted in dramatically lower concentration of this metal in the xylem sap. Further, it was demonstrated that Si(OH)(4) supplementation causes pro-reductive changes that contribute to the maintenance of Fe and, in particular, Mn in the xylem sap in bioavailable 2+ form. Our results shed more light on the intertwined reactions between Si(OH)(4) and transition metals in plant fluids (e.g. xylem sap).",
publisher = "Springer, Dordrecht",
journal = "Biometals",
title = "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study",
pages = "951-945",
number = "5",
volume = "29",
doi = "10.1007/s10534-016-9966-9"
}

Stević, N., Korać Jačić, J., Pavlović, J.,& Nikolic, M.. (2016). Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals
Springer, Dordrecht., 29(5), 945-951.
https://doi.org/10.1007/s10534-016-9966-9

Stević N, Korać Jačić J, Pavlović J, Nikolic M. Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study. in Biometals. 2016;29(5):945-951.
doi:10.1007/s10534-016-9966-9 .

Stević, Nenad, Korać Jačić, Jelena, Pavlović, Jelena, Nikolic, Miroslav, "Binding of transition metals to monosilicic acid in aqueous and xylem (Cucumis sativus L.) solutions: a low-T electron paramagnetic resonance study" in Biometals, 29, no. 5 (2016):945-951,
https://doi.org/10.1007/s10534-016-9966-9 . .

TY  - CONF
AU  - Samardzic, Jelena
AU  - pavlovic, sanja
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2492
AB  - Silicon (Si) is a beneficial element in several crops enhancing growth and alleviating different biotic
and abiotic stresses. Iron (Fe) deficiency stress in plants includes changes in the expression and the activity of certain antioxidative enzymes. Our previous study suggested the role of Si in alleviation of Fe deficiency stress by an increased root Fe acquisition (Pavlovic et al., 2013). MicroRNAs (miRNAs) are a class of endogenous small RNAs (21 to 24 nucleotides) playing a crucial role in the development of both plant and animals by down regulating gene expression at the post-transcriptional level. Here we studied the expression of miRNA398 and miRNA408 and its target gene superoxide dismutase (SOD) in Fe deficient cucumber (Cucumis sativus L.) plants grown with or without addition of Si. The expression of miRNAs and target gene was measured in roots, stems and leaves as well as in the leaves of different ages. lntercostal chlorosis in the young leaves was markedly decreased in the Si treatment. Plants treated with Si also showed increased expression of miRNA398 and miRNA408 in all organs, and consequently lower level of CuSOD transcripts. The expression of particular miRNAs followed this pattern; the highest level in the old fully developed leaves and the lowest level in young chlorotic leaves. Our results indicate that Si reduces (or at least respond on) the oxidative stress in Fe deficient cucumber plants on the posttranscriptional level mediated by miRNAs398 and 408.
PB  - IPK, Gatersleben
C3  - 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany
T1  - Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2492
ER  - 

@conference{
author = "Samardzic, Jelena and pavlovic, sanja and Timotijević, Gordana and Pavlović, Jelena and Nikolic, Miroslav",
year = "2014",
abstract = "Silicon (Si) is a beneficial element in several crops enhancing growth and alleviating different biotic
and abiotic stresses. Iron (Fe) deficiency stress in plants includes changes in the expression and the activity of certain antioxidative enzymes. Our previous study suggested the role of Si in alleviation of Fe deficiency stress by an increased root Fe acquisition (Pavlovic et al., 2013). MicroRNAs (miRNAs) are a class of endogenous small RNAs (21 to 24 nucleotides) playing a crucial role in the development of both plant and animals by down regulating gene expression at the post-transcriptional level. Here we studied the expression of miRNA398 and miRNA408 and its target gene superoxide dismutase (SOD) in Fe deficient cucumber (Cucumis sativus L.) plants grown with or without addition of Si. The expression of miRNAs and target gene was measured in roots, stems and leaves as well as in the leaves of different ages. lntercostal chlorosis in the young leaves was markedly decreased in the Si treatment. Plants treated with Si also showed increased expression of miRNA398 and miRNA408 in all organs, and consequently lower level of CuSOD transcripts. The expression of particular miRNAs followed this pattern; the highest level in the old fully developed leaves and the lowest level in young chlorotic leaves. Our results indicate that Si reduces (or at least respond on) the oxidative stress in Fe deficient cucumber plants on the posttranscriptional level mediated by miRNAs398 and 408.",
publisher = "IPK, Gatersleben",
journal = "17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany",
title = "Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2492"
}

Samardzic, J., pavlovic, s., Timotijević, G., Pavlović, J.,& Nikolic, M.. (2014). Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany
IPK, Gatersleben..
https://hdl.handle.net/21.15107/rcub_rimsi_2492

Samardzic J, pavlovic S, Timotijević G, Pavlović J, Nikolic M. Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany. 2014;.
https://hdl.handle.net/21.15107/rcub_rimsi_2492 .

Samardzic, Jelena, pavlovic, sanja, Timotijević, Gordana, Pavlović, Jelena, Nikolic, Miroslav, "Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants" in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany (2014),
https://hdl.handle.net/21.15107/rcub_rimsi_2492 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2489
AB  - Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants; however, Si is proved to alleviate multiple environmental stresses in plants. So far, Fe deficiency have mainly been studied and characterized in nutrient solution experiments devoid of Si, hence information on the interactions between these two minerals in plants is still limited. Here we investigated how Si ameliorates Fe deficiency in cucumber (model of Strategy 1 and Si-accumulating species), focusing on the mechanisms involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe, as well as on Fe distribution towards young leaves in Fe-deficient cucumber plants.
Application of Si increased the root apoplastic Fe pool, together with the enhanced expression of genes involved in reduction-based Fe uptake (FRO2, IRT1 and HA1). Moreover, in Fe deficient cucumber roots, Si influenced the genes involved in the carboxylate, shikimate and phenylpropanoid metabolism, thus resulted in enhanced accumulation of Fe chelating compounds (organic acids and phenolics) for improved Fe mobilization from the rhizosphere and reutilization of root apoplastic Fe (Pavlovic et al., 2013). Application of Si also facilitated mobility and xylem traslocation of Fe towards shoot, along with the accumulation of Fe-mobilizing compounds such as citrate in xylem sap, root and shoot tissues or cathechins  in roots (Pavlovic et al., 2013; Bityutskii et al., 2014). Very recently, we demonstrated that Si enhanced remobilization of labelled 57Fe from old leaves and its retranslocation to the younger leaves is accompanied by Si-induced expression of genes encoding both NA biosynthesis (SAM and NAS) and YSL transporters responsible for symplastic Fe unloading in leaves and floem transport of Fe-NA complex. 
In conclusion, the alleviating effect of Si seems to be more indirect, by affecting activation of Fe deficiency-related genes responsible for enhanced root acquisition and tissue mobilization of Fe. This work not only provides new evidence for the beneficial role of Si in iron nutrition, but, in perspective, can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.
PB  - IPK, Gatersleben
C3  - 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany
T1  - Interactions between iron and silicon in cucumber
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2489
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Nikolic, Miroslav",
year = "2014",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants; however, Si is proved to alleviate multiple environmental stresses in plants. So far, Fe deficiency have mainly been studied and characterized in nutrient solution experiments devoid of Si, hence information on the interactions between these two minerals in plants is still limited. Here we investigated how Si ameliorates Fe deficiency in cucumber (model of Strategy 1 and Si-accumulating species), focusing on the mechanisms involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe, as well as on Fe distribution towards young leaves in Fe-deficient cucumber plants.
Application of Si increased the root apoplastic Fe pool, together with the enhanced expression of genes involved in reduction-based Fe uptake (FRO2, IRT1 and HA1). Moreover, in Fe deficient cucumber roots, Si influenced the genes involved in the carboxylate, shikimate and phenylpropanoid metabolism, thus resulted in enhanced accumulation of Fe chelating compounds (organic acids and phenolics) for improved Fe mobilization from the rhizosphere and reutilization of root apoplastic Fe (Pavlovic et al., 2013). Application of Si also facilitated mobility and xylem traslocation of Fe towards shoot, along with the accumulation of Fe-mobilizing compounds such as citrate in xylem sap, root and shoot tissues or cathechins  in roots (Pavlovic et al., 2013; Bityutskii et al., 2014). Very recently, we demonstrated that Si enhanced remobilization of labelled 57Fe from old leaves and its retranslocation to the younger leaves is accompanied by Si-induced expression of genes encoding both NA biosynthesis (SAM and NAS) and YSL transporters responsible for symplastic Fe unloading in leaves and floem transport of Fe-NA complex. 
In conclusion, the alleviating effect of Si seems to be more indirect, by affecting activation of Fe deficiency-related genes responsible for enhanced root acquisition and tissue mobilization of Fe. This work not only provides new evidence for the beneficial role of Si in iron nutrition, but, in perspective, can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.",
publisher = "IPK, Gatersleben",
journal = "17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany",
title = "Interactions between iron and silicon in cucumber",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2489"
}

Pavlović, J., Samardzic, J.,& Nikolic, M.. (2014). Interactions between iron and silicon in cucumber. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany
IPK, Gatersleben..
https://hdl.handle.net/21.15107/rcub_rimsi_2489

Pavlović J, Samardzic J, Nikolic M. Interactions between iron and silicon in cucumber. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany. 2014;.
https://hdl.handle.net/21.15107/rcub_rimsi_2489 .

Pavlović, Jelena, Samardzic, Jelena, Nikolic, Miroslav, "Interactions between iron and silicon in cucumber" in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany (2014),
https://hdl.handle.net/21.15107/rcub_rimsi_2489 .

TY  - JOUR
AU  - Bityutskii, Nikolai
AU  - Pavlović, Jelena
AU  - Yakkonen, Kirill
AU  - Maksimović, Vuk
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/763
AB  - Although the beneficial role of silicon (Si) in alleviation of abiotic stress is well established, little is known of the relevance of Si nutrition under microelement deficiency. The aim of our work was to investigate the physiological role of Si in relation to micronutrient (Fe, Zn and Mn) deficiencies in cucumber (Cucumis sativus L). Cucumber (cv. Semkross) plants were grown hydroponically in a complete nutrient solution (control) and in nutrient solutions free from Fe, Zn or Mn, with or without Si supply. Plant tissue concentrations of microelements, organic acids and phenolics were measured. Si supply effectively mitigated the symptoms of Fe deficiency, but only in part, the symptoms of Zn- or Mn deficiency. Leaf Fe concentration significantly increased in plants deprived of Fe but treated with Si, whereas the concentrations of other microelements were not affected by Si supply. The effects of Si supply in increasing accumulation of both organic acids and phenolic compounds in cucumber tissues were exclusively related to Fe nutrition. Enhancement of Fe distribution towards apical shoot parts, along with the tissue accumulation of Fe-mobilizing compounds such as citrate (in leaves and roots) or cathechin (in roots) appears to be the major alleviating effect of Si. Si nutrition, however, was without effect on the mobility and tissue distribution of either Zn or Mn.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber
EP  - 211
SP  - 205
VL  - 74
DO  - 10.1016/j.plaphy.2013.11.015
ER  - 

@article{
author = "Bityutskii, Nikolai and Pavlović, Jelena and Yakkonen, Kirill and Maksimović, Vuk and Nikolic, Miroslav",
year = "2014",
abstract = "Although the beneficial role of silicon (Si) in alleviation of abiotic stress is well established, little is known of the relevance of Si nutrition under microelement deficiency. The aim of our work was to investigate the physiological role of Si in relation to micronutrient (Fe, Zn and Mn) deficiencies in cucumber (Cucumis sativus L). Cucumber (cv. Semkross) plants were grown hydroponically in a complete nutrient solution (control) and in nutrient solutions free from Fe, Zn or Mn, with or without Si supply. Plant tissue concentrations of microelements, organic acids and phenolics were measured. Si supply effectively mitigated the symptoms of Fe deficiency, but only in part, the symptoms of Zn- or Mn deficiency. Leaf Fe concentration significantly increased in plants deprived of Fe but treated with Si, whereas the concentrations of other microelements were not affected by Si supply. The effects of Si supply in increasing accumulation of both organic acids and phenolic compounds in cucumber tissues were exclusively related to Fe nutrition. Enhancement of Fe distribution towards apical shoot parts, along with the tissue accumulation of Fe-mobilizing compounds such as citrate (in leaves and roots) or cathechin (in roots) appears to be the major alleviating effect of Si. Si nutrition, however, was without effect on the mobility and tissue distribution of either Zn or Mn.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber",
pages = "211-205",
volume = "74",
doi = "10.1016/j.plaphy.2013.11.015"
}

Bityutskii, N., Pavlović, J., Yakkonen, K., Maksimović, V.,& Nikolic, M.. (2014). Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 74, 205-211.
https://doi.org/10.1016/j.plaphy.2013.11.015

Bityutskii N, Pavlović J, Yakkonen K, Maksimović V, Nikolic M. Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber. in Plant Physiology and Biochemistry. 2014;74:205-211.
doi:10.1016/j.plaphy.2013.11.015 .

Bityutskii, Nikolai, Pavlović, Jelena, Yakkonen, Kirill, Maksimović, Vuk, Nikolic, Miroslav, "Contrasting effect of silicon on iron, zinc and manganese status and accumulation of metal-mobilizing compounds in micronutrient-deficient cucumber" in Plant Physiology and Biochemistry, 74 (2014):205-211,
https://doi.org/10.1016/j.plaphy.2013.11.015 . .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Maksimović, Vuk
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2496
AB  - Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth's crust. While the essentiality of Fe is discovered at the middle of the 19111 century, Si is still not fully accepted as an essential element for higher plants. However, Si is proved to alleviate multiple environmental stresses in plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency is considered to be one of the major limiting factors for crop production worldwide, also affecting human health in developing countries.
Root responses (strategies) to a lack of Fe have mainly been studied in nutrient solution experiments devoid of Si, therefore information on the interactions between these two mineral elements in plants is still limited.
Here we investigated how Si ameliorates Fe deficiency in Strategy 1 plant species (all dicots and monocots with the exception of grasses, which belong to Strategy 2), such as cucumber, sunflower, tomato and soybean, with the focus on the mechanism involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe. A combined approach was performed including analyzes of apoplastic Fe pool, the components of reduction-based Fe acquisition machinery (using stable isotope 57Fe and expression of CsFR02, Cs/RT1, and CsHA 7) and accumulation of Fe-mobilizing compounds (carboxylates, phenolics and flavonoids), along with
the expression of related genes involved in their biosynthesis, in the roots of model plant (cucumber).
Our study indicates for the first time that the role of Si in the alleviation of Fe deficiency stress includes: 1) increase of the apoplastic Fe pool in roots; 2) stimulation of Fe acquisition at the ea rly stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in this process; and 3) increase of the accumulation of Fe-mobilizing compounds in roots. Indeed, this work provides new evidence for the beneficial role of Si in plant nutrition and in perspective can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.
PB  - Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - Silicon mediates iron acquisition by Strategy 1 plants
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2496
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Maksimović, Vuk and Nikolic, Miroslav",
year = "2013",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth's crust. While the essentiality of Fe is discovered at the middle of the 19111 century, Si is still not fully accepted as an essential element for higher plants. However, Si is proved to alleviate multiple environmental stresses in plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency is considered to be one of the major limiting factors for crop production worldwide, also affecting human health in developing countries.
Root responses (strategies) to a lack of Fe have mainly been studied in nutrient solution experiments devoid of Si, therefore information on the interactions between these two mineral elements in plants is still limited.
Here we investigated how Si ameliorates Fe deficiency in Strategy 1 plant species (all dicots and monocots with the exception of grasses, which belong to Strategy 2), such as cucumber, sunflower, tomato and soybean, with the focus on the mechanism involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe. A combined approach was performed including analyzes of apoplastic Fe pool, the components of reduction-based Fe acquisition machinery (using stable isotope 57Fe and expression of CsFR02, Cs/RT1, and CsHA 7) and accumulation of Fe-mobilizing compounds (carboxylates, phenolics and flavonoids), along with
the expression of related genes involved in their biosynthesis, in the roots of model plant (cucumber).
Our study indicates for the first time that the role of Si in the alleviation of Fe deficiency stress includes: 1) increase of the apoplastic Fe pool in roots; 2) stimulation of Fe acquisition at the ea rly stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in this process; and 3) increase of the accumulation of Fe-mobilizing compounds in roots. Indeed, this work provides new evidence for the beneficial role of Si in plant nutrition and in perspective can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.",
publisher = "Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "Silicon mediates iron acquisition by Strategy 1 plants",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2496"
}

Pavlović, J., Samardzic, J., Maksimović, V.,& Nikolic, M.. (2013). Silicon mediates iron acquisition by Strategy 1 plants. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia..
https://hdl.handle.net/21.15107/rcub_rimsi_2496

Pavlović J, Samardzic J, Maksimović V, Nikolic M. Silicon mediates iron acquisition by Strategy 1 plants. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2496 .

Pavlović, Jelena, Samardzic, Jelena, Maksimović, Vuk, Nikolic, Miroslav, "Silicon mediates iron acquisition by Strategy 1 plants" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2496 .

TY  - CONF
AU  - Bosnić, Predrag
AU  - Savic, Jasna
AU  - Kostić, Ljiljana
AU  - Stevic, Nenad
AU  - Pavlović, Jelena
AU  - Lazic, Marina
AU  - Marjanovic Jeromela, Ana
AU  - Hristov, Nikola
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2504
AB  - Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2504
ER  - 

@conference{
author = "Bosnić, Predrag and Savic, Jasna and Kostić, Ljiljana and Stevic, Nenad and Pavlović, Jelena and Lazic, Marina and Marjanovic Jeromela, Ana and Hristov, Nikola and Nikolić, Nina and Nikolic, Miroslav",
year = "2013",
abstract = "Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2504"
}

Bosnić, P., Savic, J., Kostić, L., Stevic, N., Pavlović, J., Lazic, M., Marjanovic Jeromela, A., Hristov, N., Nikolić, N.,& Nikolic, M.. (2013). Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2504

Bosnić P, Savic J, Kostić L, Stevic N, Pavlović J, Lazic M, Marjanovic Jeromela A, Hristov N, Nikolić N, Nikolic M. Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

Bosnić, Predrag, Savic, Jasna, Kostić, Ljiljana, Stevic, Nenad, Pavlović, Jelena, Lazic, Marina, Marjanovic Jeromela, Ana, Hristov, Nikola, Nikolić, Nina, Nikolic, Miroslav, "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

TY  - CONF
AU  - Stevic, Nenad
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2500
AB  - Recently, we proposed the presence of iron (Fe)-polysilicate chelate-like complexes as one of the possible mechanism involved in silicon (Si)-mediated alleviation of Fe deficiency in plants (see Pavlovic et al., this volume). The presence of metals and organic ligands (e.g. oxalate and citrate) in aqueous solution can result in the complexation with dissolved silica. In aqueous solutions with low dissolved silica concentration (<10 mM) the principal species are monomeric (e.g. H4Si04(aq), H3Si04·, H2Si0/ ), and polymeric forms to various extents. The anionic species Sil/ which is formed according to the reaction: Si(OH)4 + 3H2L =SiL32-+ 2H+ + 4H20, is a hexacoordinated charge-transfer complex in which the ligands (L) are bidentately bound. A likely assumption is that the main interactions between Si and polycarboxylates do not take place in the aqueous phase,
I nit rather on the surface of the dissolved particles (such as the surface of plant cell walls).
As a strong Fe chelator, citrate (Cit4-) has been considered as the most likely major candidate for Fe transport in plants via xylem and apoplastic fluids. Ferric-citrate chemistry is complicated and a definitive description of Its aqueous speciation at the neutral or weakly acidic pH va lues (typical for plant fluids) remains elusive. The predominant Fe(lll)-citrate species at the physiological pH values are the mononuclear biologically relevant dicitrate [Fe(Cit)2)5- complex and multinuclear species of low nuclearity, in particular trinuclear complexes
The [Fe(Cit)i]5· complex predominates in the Fe: citrate molar ratio range 1:100 to 1:10 (the normal range found in the apoplastic fluids of Fe-adequate plants).
Aqueous silica forms stable complexes with polymeric ferric oxy-hydroxide species, which may be formed in the root apoplast. The structure of the Fe-Si complexes appears to be similar at both acid and alkaline pH. The presence of single-corner Fe-0-Si bonds is expected in the monomer complex FeOSi(OH)3 2+ in the acidic solutions. Silica substitutes for double-corner FeO6-octahedra in Fe oxy-hydroxide polymeric complexes existing in the early stages of Fe(III) hydrolysis, likely by forming 2C-type (double corner) complexes with small Fe oxy-hydroxide polymers whose structure consists of Fe06-octahedra linked together by common edges. In
low activities aqueous silica solutions, Fe3+ remains essentially hexa-coordinated.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - The theoretical prediction of interactions between soluble silicon, iron (III) and carboxylate anions in plant fluids
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2500
ER  - 

@conference{
author = "Stevic, Nenad and Pavlović, Jelena and Nikolic, Miroslav",
year = "2013",
abstract = "Recently, we proposed the presence of iron (Fe)-polysilicate chelate-like complexes as one of the possible mechanism involved in silicon (Si)-mediated alleviation of Fe deficiency in plants (see Pavlovic et al., this volume). The presence of metals and organic ligands (e.g. oxalate and citrate) in aqueous solution can result in the complexation with dissolved silica. In aqueous solutions with low dissolved silica concentration (<10 mM) the principal species are monomeric (e.g. H4Si04(aq), H3Si04·, H2Si0/ ), and polymeric forms to various extents. The anionic species Sil/ which is formed according to the reaction: Si(OH)4 + 3H2L =SiL32-+ 2H+ + 4H20, is a hexacoordinated charge-transfer complex in which the ligands (L) are bidentately bound. A likely assumption is that the main interactions between Si and polycarboxylates do not take place in the aqueous phase,
I nit rather on the surface of the dissolved particles (such as the surface of plant cell walls).
As a strong Fe chelator, citrate (Cit4-) has been considered as the most likely major candidate for Fe transport in plants via xylem and apoplastic fluids. Ferric-citrate chemistry is complicated and a definitive description of Its aqueous speciation at the neutral or weakly acidic pH va lues (typical for plant fluids) remains elusive. The predominant Fe(lll)-citrate species at the physiological pH values are the mononuclear biologically relevant dicitrate [Fe(Cit)2)5- complex and multinuclear species of low nuclearity, in particular trinuclear complexes
The [Fe(Cit)i]5· complex predominates in the Fe: citrate molar ratio range 1:100 to 1:10 (the normal range found in the apoplastic fluids of Fe-adequate plants).
Aqueous silica forms stable complexes with polymeric ferric oxy-hydroxide species, which may be formed in the root apoplast. The structure of the Fe-Si complexes appears to be similar at both acid and alkaline pH. The presence of single-corner Fe-0-Si bonds is expected in the monomer complex FeOSi(OH)3 2+ in the acidic solutions. Silica substitutes for double-corner FeO6-octahedra in Fe oxy-hydroxide polymeric complexes existing in the early stages of Fe(III) hydrolysis, likely by forming 2C-type (double corner) complexes with small Fe oxy-hydroxide polymers whose structure consists of Fe06-octahedra linked together by common edges. In
low activities aqueous silica solutions, Fe3+ remains essentially hexa-coordinated.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "The theoretical prediction of interactions between soluble silicon, iron (III) and carboxylate anions in plant fluids",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2500"
}

Stevic, N., Pavlović, J.,& Nikolic, M.. (2013). The theoretical prediction of interactions between soluble silicon, iron (III) and carboxylate anions in plant fluids. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2500

Stevic N, Pavlović J, Nikolic M. The theoretical prediction of interactions between soluble silicon, iron (III) and carboxylate anions in plant fluids. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2500 .

Stevic, Nenad, Pavlović, Jelena, Nikolic, Miroslav, "The theoretical prediction of interactions between soluble silicon, iron (III) and carboxylate anions in plant fluids" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2500 .

TY  - CONF
AU  - Nikolic, Dragana S.
AU  - Nikolic, Dragana B.
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2515
AB  - Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
T1  - Silicon mitigates oxidative stress in cucumber at copper excess
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2515
ER  - 

@conference{
author = "Nikolic, Dragana S. and Nikolic, Dragana B. and Timotijević, Gordana and Pavlović, Jelena and Samardzic, Jelena and Nikolic, Miroslav",
year = "2013",
abstract = "Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia",
title = "Silicon mitigates oxidative stress in cucumber at copper excess",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2515"
}

Nikolic, D. S., Nikolic, D. B., Timotijević, G., Pavlović, J., Samardzic, J.,& Nikolic, M.. (2013). Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2515

Nikolic DS, Nikolic DB, Timotijević G, Pavlović J, Samardzic J, Nikolic M. Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .

Nikolic, Dragana S., Nikolic, Dragana B., Timotijević, Gordana, Pavlović, Jelena, Samardzic, Jelena, Nikolic, Miroslav, "Silicon mitigates oxidative stress in cucumber at copper excess" in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Maksimović, Vuk
AU  - Timotijević, Gordana
AU  - Stević, Nenad M
AU  - Laursen, Kristian H
AU  - Hansen, Thomas H
AU  - Husted, Soren
AU  - Schjoerring, Jan K
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/665
AB  - Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.
PB  - Wiley, Hoboken
T2  - New Phytologist
T1  - Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast
EP  - 1107
IS  - 4
SP  - 1096
VL  - 198
DO  - 10.1111/nph.12213
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Maksimović, Vuk and Timotijević, Gordana and Stević, Nenad M and Laursen, Kristian H and Hansen, Thomas H and Husted, Soren and Schjoerring, Jan K and Liang, Yongchao and Nikolic, Miroslav",
year = "2013",
abstract = "Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.",
publisher = "Wiley, Hoboken",
journal = "New Phytologist",
title = "Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast",
pages = "1107-1096",
number = "4",
volume = "198",
doi = "10.1111/nph.12213"
}

Pavlović, J., Samardžić, J. T., Maksimović, V., Timotijević, G., Stević, N. M., Laursen, K. H., Hansen, T. H., Husted, S., Schjoerring, J. K., Liang, Y.,& Nikolic, M.. (2013). Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast. in New Phytologist
Wiley, Hoboken., 198(4), 1096-1107.
https://doi.org/10.1111/nph.12213

Pavlović J, Samardžić JT, Maksimović V, Timotijević G, Stević NM, Laursen KH, Hansen TH, Husted S, Schjoerring JK, Liang Y, Nikolic M. Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast. in New Phytologist. 2013;198(4):1096-1107.
doi:10.1111/nph.12213 .

Pavlović, Jelena, Samardžić, Jelena T., Maksimović, Vuk, Timotijević, Gordana, Stević, Nenad M, Laursen, Kristian H, Hansen, Thomas H, Husted, Soren, Schjoerring, Jan K, Liang, Yongchao, Nikolic, Miroslav, "Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast" in New Phytologist, 198, no. 4 (2013):1096-1107,
https://doi.org/10.1111/nph.12213 . .