TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Nikolic, Dragana B.
AU  - Milovanović, Bosko M.
AU  - Nikolic, Dragana S.
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/848
AB  - MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis
EP  - 416
IS  - 2
SP  - 405
VL  - 47
DO  - 10.2298/GENSR1502405T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Nikolic, Dragana B. and Milovanović, Bosko M. and Nikolic, Dragana S. and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2015",
abstract = "MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis",
pages = "416-405",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502405T"
}

Timotijević, G., Milisavljević, M., Nikolic, D. B., Milovanović, B. M., Nikolic, D. S., Nikolic, M.,& Samardžić, J. T.. (2015). Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 405-416.
https://doi.org/10.2298/GENSR1502405T

Timotijević G, Milisavljević M, Nikolic DB, Milovanović BM, Nikolic DS, Nikolic M, Samardžić JT. Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade. 2015;47(2):405-416.
doi:10.2298/GENSR1502405T .

Timotijević, Gordana, Milisavljević, Mira, Nikolic, Dragana B., Milovanović, Bosko M., Nikolic, Dragana S., Nikolic, Miroslav, Samardžić, Jelena T., "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis" in Genetika-Belgrade, 47, no. 2 (2015):405-416,
https://doi.org/10.2298/GENSR1502405T . .

TY  - CONF
AU  - Nikolic, Dragana S.
AU  - Nikolic, Dragana B.
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2515
AB  - Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
T1  - Silicon mitigates oxidative stress in cucumber at copper excess
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2515
ER  - 

@conference{
author = "Nikolic, Dragana S. and Nikolic, Dragana B. and Timotijević, Gordana and Pavlović, Jelena and Samardzic, Jelena and Nikolic, Miroslav",
year = "2013",
abstract = "Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia",
title = "Silicon mitigates oxidative stress in cucumber at copper excess",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2515"
}

Nikolic, D. S., Nikolic, D. B., Timotijević, G., Pavlović, J., Samardzic, J.,& Nikolic, M.. (2013). Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2515

Nikolic DS, Nikolic DB, Timotijević G, Pavlović J, Samardzic J, Nikolic M. Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .

Nikolic, Dragana S., Nikolic, Dragana B., Timotijević, Gordana, Pavlović, Jelena, Samardzic, Jelena, Nikolic, Miroslav, "Silicon mitigates oxidative stress in cucumber at copper excess" in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .