TY  - CONF
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardzic, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2478
AB  - The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.
PB  - University of Copenhagen, Denmark
C3  - Plant Biology Europe 2018 Conference Abstract Book
T1  - The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2478
ER  - 

@conference{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić, Ljiljana and Nikolic, Miroslav and Samardzic, Jelena",
year = "2018",
abstract = "The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.",
publisher = "University of Copenhagen, Denmark",
journal = "Plant Biology Europe 2018 Conference Abstract Book",
title = "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2478"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić, L., Nikolic, M.,& Samardzic, J.. (2018). The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2478

Nikolic DB, Nesic S, Bosnic D, Kostić L, Nikolic M, Samardzic J. The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić, Ljiljana, Nikolic, Miroslav, Samardzic, Jelena, "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution" in Plant Biology Europe 2018 Conference Abstract Book (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

TY  - CONF
AU  - Samardzic, Jelena
AU  - pavlovic, sanja
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2492
AB  - Silicon (Si) is a beneficial element in several crops enhancing growth and alleviating different biotic
and abiotic stresses. Iron (Fe) deficiency stress in plants includes changes in the expression and the activity of certain antioxidative enzymes. Our previous study suggested the role of Si in alleviation of Fe deficiency stress by an increased root Fe acquisition (Pavlovic et al., 2013). MicroRNAs (miRNAs) are a class of endogenous small RNAs (21 to 24 nucleotides) playing a crucial role in the development of both plant and animals by down regulating gene expression at the post-transcriptional level. Here we studied the expression of miRNA398 and miRNA408 and its target gene superoxide dismutase (SOD) in Fe deficient cucumber (Cucumis sativus L.) plants grown with or without addition of Si. The expression of miRNAs and target gene was measured in roots, stems and leaves as well as in the leaves of different ages. lntercostal chlorosis in the young leaves was markedly decreased in the Si treatment. Plants treated with Si also showed increased expression of miRNA398 and miRNA408 in all organs, and consequently lower level of CuSOD transcripts. The expression of particular miRNAs followed this pattern; the highest level in the old fully developed leaves and the lowest level in young chlorotic leaves. Our results indicate that Si reduces (or at least respond on) the oxidative stress in Fe deficient cucumber plants on the posttranscriptional level mediated by miRNAs398 and 408.
PB  - IPK, Gatersleben
C3  - 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany
T1  - Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2492
ER  - 

@conference{
author = "Samardzic, Jelena and pavlovic, sanja and Timotijević, Gordana and Pavlović, Jelena and Nikolic, Miroslav",
year = "2014",
abstract = "Silicon (Si) is a beneficial element in several crops enhancing growth and alleviating different biotic
and abiotic stresses. Iron (Fe) deficiency stress in plants includes changes in the expression and the activity of certain antioxidative enzymes. Our previous study suggested the role of Si in alleviation of Fe deficiency stress by an increased root Fe acquisition (Pavlovic et al., 2013). MicroRNAs (miRNAs) are a class of endogenous small RNAs (21 to 24 nucleotides) playing a crucial role in the development of both plant and animals by down regulating gene expression at the post-transcriptional level. Here we studied the expression of miRNA398 and miRNA408 and its target gene superoxide dismutase (SOD) in Fe deficient cucumber (Cucumis sativus L.) plants grown with or without addition of Si. The expression of miRNAs and target gene was measured in roots, stems and leaves as well as in the leaves of different ages. lntercostal chlorosis in the young leaves was markedly decreased in the Si treatment. Plants treated with Si also showed increased expression of miRNA398 and miRNA408 in all organs, and consequently lower level of CuSOD transcripts. The expression of particular miRNAs followed this pattern; the highest level in the old fully developed leaves and the lowest level in young chlorotic leaves. Our results indicate that Si reduces (or at least respond on) the oxidative stress in Fe deficient cucumber plants on the posttranscriptional level mediated by miRNAs398 and 408.",
publisher = "IPK, Gatersleben",
journal = "17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany",
title = "Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2492"
}

Samardzic, J., pavlovic, s., Timotijević, G., Pavlović, J.,& Nikolic, M.. (2014). Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany
IPK, Gatersleben..
https://hdl.handle.net/21.15107/rcub_rimsi_2492

Samardzic J, pavlovic S, Timotijević G, Pavlović J, Nikolic M. Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany. 2014;.
https://hdl.handle.net/21.15107/rcub_rimsi_2492 .

Samardzic, Jelena, pavlovic, sanja, Timotijević, Gordana, Pavlović, Jelena, Nikolic, Miroslav, "Effects of Si on the expression of miRNA398 and miRNA408 and its target gene, superoxide dismutase (CuSOD) in Fe deficient cucumber plants" in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben, Germany (2014),
https://hdl.handle.net/21.15107/rcub_rimsi_2492 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2489
AB  - Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants; however, Si is proved to alleviate multiple environmental stresses in plants. So far, Fe deficiency have mainly been studied and characterized in nutrient solution experiments devoid of Si, hence information on the interactions between these two minerals in plants is still limited. Here we investigated how Si ameliorates Fe deficiency in cucumber (model of Strategy 1 and Si-accumulating species), focusing on the mechanisms involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe, as well as on Fe distribution towards young leaves in Fe-deficient cucumber plants.
Application of Si increased the root apoplastic Fe pool, together with the enhanced expression of genes involved in reduction-based Fe uptake (FRO2, IRT1 and HA1). Moreover, in Fe deficient cucumber roots, Si influenced the genes involved in the carboxylate, shikimate and phenylpropanoid metabolism, thus resulted in enhanced accumulation of Fe chelating compounds (organic acids and phenolics) for improved Fe mobilization from the rhizosphere and reutilization of root apoplastic Fe (Pavlovic et al., 2013). Application of Si also facilitated mobility and xylem traslocation of Fe towards shoot, along with the accumulation of Fe-mobilizing compounds such as citrate in xylem sap, root and shoot tissues or cathechins  in roots (Pavlovic et al., 2013; Bityutskii et al., 2014). Very recently, we demonstrated that Si enhanced remobilization of labelled 57Fe from old leaves and its retranslocation to the younger leaves is accompanied by Si-induced expression of genes encoding both NA biosynthesis (SAM and NAS) and YSL transporters responsible for symplastic Fe unloading in leaves and floem transport of Fe-NA complex. 
In conclusion, the alleviating effect of Si seems to be more indirect, by affecting activation of Fe deficiency-related genes responsible for enhanced root acquisition and tissue mobilization of Fe. This work not only provides new evidence for the beneficial role of Si in iron nutrition, but, in perspective, can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.
PB  - IPK, Gatersleben
C3  - 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany
T1  - Interactions between iron and silicon in cucumber
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2489
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Nikolic, Miroslav",
year = "2014",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth’s crust. While the essentiality of Fe has been discovered in the middle of the 19th century, Si is still not fully accepted as an essential element for higher plants; however, Si is proved to alleviate multiple environmental stresses in plants. So far, Fe deficiency have mainly been studied and characterized in nutrient solution experiments devoid of Si, hence information on the interactions between these two minerals in plants is still limited. Here we investigated how Si ameliorates Fe deficiency in cucumber (model of Strategy 1 and Si-accumulating species), focusing on the mechanisms involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe, as well as on Fe distribution towards young leaves in Fe-deficient cucumber plants.
Application of Si increased the root apoplastic Fe pool, together with the enhanced expression of genes involved in reduction-based Fe uptake (FRO2, IRT1 and HA1). Moreover, in Fe deficient cucumber roots, Si influenced the genes involved in the carboxylate, shikimate and phenylpropanoid metabolism, thus resulted in enhanced accumulation of Fe chelating compounds (organic acids and phenolics) for improved Fe mobilization from the rhizosphere and reutilization of root apoplastic Fe (Pavlovic et al., 2013). Application of Si also facilitated mobility and xylem traslocation of Fe towards shoot, along with the accumulation of Fe-mobilizing compounds such as citrate in xylem sap, root and shoot tissues or cathechins  in roots (Pavlovic et al., 2013; Bityutskii et al., 2014). Very recently, we demonstrated that Si enhanced remobilization of labelled 57Fe from old leaves and its retranslocation to the younger leaves is accompanied by Si-induced expression of genes encoding both NA biosynthesis (SAM and NAS) and YSL transporters responsible for symplastic Fe unloading in leaves and floem transport of Fe-NA complex. 
In conclusion, the alleviating effect of Si seems to be more indirect, by affecting activation of Fe deficiency-related genes responsible for enhanced root acquisition and tissue mobilization of Fe. This work not only provides new evidence for the beneficial role of Si in iron nutrition, but, in perspective, can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.",
publisher = "IPK, Gatersleben",
journal = "17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany",
title = "Interactions between iron and silicon in cucumber",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2489"
}

Pavlović, J., Samardzic, J.,& Nikolic, M.. (2014). Interactions between iron and silicon in cucumber. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany
IPK, Gatersleben..
https://hdl.handle.net/21.15107/rcub_rimsi_2489

Pavlović J, Samardzic J, Nikolic M. Interactions between iron and silicon in cucumber. in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany. 2014;.
https://hdl.handle.net/21.15107/rcub_rimsi_2489 .

Pavlović, Jelena, Samardzic, Jelena, Nikolic, Miroslav, "Interactions between iron and silicon in cucumber" in 17th International Symposium on Iron Nutrition and Interaction in Plants - Program Book, Gatersleben / Quedlinburg, Germany (2014),
https://hdl.handle.net/21.15107/rcub_rimsi_2489 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Maksimović, Vuk
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2496
AB  - Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth's crust. While the essentiality of Fe is discovered at the middle of the 19111 century, Si is still not fully accepted as an essential element for higher plants. However, Si is proved to alleviate multiple environmental stresses in plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency is considered to be one of the major limiting factors for crop production worldwide, also affecting human health in developing countries.
Root responses (strategies) to a lack of Fe have mainly been studied in nutrient solution experiments devoid of Si, therefore information on the interactions between these two mineral elements in plants is still limited.
Here we investigated how Si ameliorates Fe deficiency in Strategy 1 plant species (all dicots and monocots with the exception of grasses, which belong to Strategy 2), such as cucumber, sunflower, tomato and soybean, with the focus on the mechanism involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe. A combined approach was performed including analyzes of apoplastic Fe pool, the components of reduction-based Fe acquisition machinery (using stable isotope 57Fe and expression of CsFR02, Cs/RT1, and CsHA 7) and accumulation of Fe-mobilizing compounds (carboxylates, phenolics and flavonoids), along with
the expression of related genes involved in their biosynthesis, in the roots of model plant (cucumber).
Our study indicates for the first time that the role of Si in the alleviation of Fe deficiency stress includes: 1) increase of the apoplastic Fe pool in roots; 2) stimulation of Fe acquisition at the ea rly stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in this process; and 3) increase of the accumulation of Fe-mobilizing compounds in roots. Indeed, this work provides new evidence for the beneficial role of Si in plant nutrition and in perspective can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.
PB  - Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - Silicon mediates iron acquisition by Strategy 1 plants
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2496
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Maksimović, Vuk and Nikolic, Miroslav",
year = "2013",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the fourth most abundant minerals in the earth's crust. While the essentiality of Fe is discovered at the middle of the 19111 century, Si is still not fully accepted as an essential element for higher plants. However, Si is proved to alleviate multiple environmental stresses in plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency is considered to be one of the major limiting factors for crop production worldwide, also affecting human health in developing countries.
Root responses (strategies) to a lack of Fe have mainly been studied in nutrient solution experiments devoid of Si, therefore information on the interactions between these two mineral elements in plants is still limited.
Here we investigated how Si ameliorates Fe deficiency in Strategy 1 plant species (all dicots and monocots with the exception of grasses, which belong to Strategy 2), such as cucumber, sunflower, tomato and soybean, with the focus on the mechanism involved in Fe acquisition from the rhizosphere and utilization of root apoplastic Fe. A combined approach was performed including analyzes of apoplastic Fe pool, the components of reduction-based Fe acquisition machinery (using stable isotope 57Fe and expression of CsFR02, Cs/RT1, and CsHA 7) and accumulation of Fe-mobilizing compounds (carboxylates, phenolics and flavonoids), along with
the expression of related genes involved in their biosynthesis, in the roots of model plant (cucumber).
Our study indicates for the first time that the role of Si in the alleviation of Fe deficiency stress includes: 1) increase of the apoplastic Fe pool in roots; 2) stimulation of Fe acquisition at the ea rly stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in this process; and 3) increase of the accumulation of Fe-mobilizing compounds in roots. Indeed, this work provides new evidence for the beneficial role of Si in plant nutrition and in perspective can be of practical importance in the development of new sustainable measures for controlling Fe chlorosis in calcareous soils, which in general are low in available Si.",
publisher = "Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "Silicon mediates iron acquisition by Strategy 1 plants",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2496"
}

Pavlović, J., Samardzic, J., Maksimović, V.,& Nikolic, M.. (2013). Silicon mediates iron acquisition by Strategy 1 plants. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biologica l Research ,,Sinisa Stankovic", University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia..
https://hdl.handle.net/21.15107/rcub_rimsi_2496

Pavlović J, Samardzic J, Maksimović V, Nikolic M. Silicon mediates iron acquisition by Strategy 1 plants. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2496 .

Pavlović, Jelena, Samardzic, Jelena, Maksimović, Vuk, Nikolic, Miroslav, "Silicon mediates iron acquisition by Strategy 1 plants" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2496 .

TY  - CONF
AU  - Kostić, Ljiljana
AU  - Samardzic, Jelena
AU  - Nikolić, Nina
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2488
AB  - Pollution from industrial activities is increasingly creating marginal conditions for crop production worldwide. Nutrient deficiency, and in particular phosphorus (P) deficiency is an often overlooked factor which can be a severe constraint for plant growth in soils affected my mining activates (Nikolic et al., 2011). The root exudation of carboxylates (mainly citrate and malate) has been considered as the major root response to mobilize sparingly soluble P in the rhizosphere. Therefore the enhanced activity of phosphoenolpyruvate carboxylase (PEPC) appears to be a key P-independent metabolic bypass reaction of malate/citrate biosynthesis in P deficient plants. Although the beneficial role of silicon (Si) on plant growth has been reported on several plant species grown under P deficient conditions the underlying mechanism is still unknown. 
The pot experiments were conducted with winter wheat, including Si fertilizers in addition to the conventional reclamation amendments (e.g. cow manure, NPK and lime) in the acid soil polluted by sulphidic mine tailings (collected form the Timok floodplain). The main focus of this study was on the dynamics of P pools in wheat rhizosphere, and on the molecular mechanism of root response to P availability in the rhizosphere (expression of TaPT1 and TaPT2, encoding Pi transporters, TaPEPC encoding PEPC, and MATE-family genes encoding Al-activated citrate efflux transporter). All the amendments induced a significant change in the rhizosphere P fractions (readily available, Al- and Fe-bound P). For instance, Si supply has caused significant increase in readily available P, and reduction in Al-P and Fe-P. This can be attributed to synergetic effect of pH increase and reduction of P sorption by Al- and Fe-oxides. The leaf P concentration in wheat plants treated with Si significantly increased and was in the range of leaf P concentration in P-fertilized plants. While the expressions of the root TaPT2 and TaPEPC were down-regulated by P availability in the rhizosphere and plant P status, TaPT1 and TaMATE showed different pattern with markedly enhanced expression at Si treatment irrespectively of the P supply. In conclusion, Si nutrition effectively alleviates P deficiency in wheat by 1) increased P availability in rhizosphere, most probably due to MATE-mediated citrate exudation, and 2) enhanced P acquisition as a consequence of Si-promoted expressions of PT1 transporter in root plasma membrane.
PB  - Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade
C3  - 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48
T1  - The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2488
ER  - 

@conference{
author = "Kostić, Ljiljana and Samardzic, Jelena and Nikolić, Nina and Liang, Yongchao and Nikolic, Miroslav",
year = "2013",
abstract = "Pollution from industrial activities is increasingly creating marginal conditions for crop production worldwide. Nutrient deficiency, and in particular phosphorus (P) deficiency is an often overlooked factor which can be a severe constraint for plant growth in soils affected my mining activates (Nikolic et al., 2011). The root exudation of carboxylates (mainly citrate and malate) has been considered as the major root response to mobilize sparingly soluble P in the rhizosphere. Therefore the enhanced activity of phosphoenolpyruvate carboxylase (PEPC) appears to be a key P-independent metabolic bypass reaction of malate/citrate biosynthesis in P deficient plants. Although the beneficial role of silicon (Si) on plant growth has been reported on several plant species grown under P deficient conditions the underlying mechanism is still unknown. 
The pot experiments were conducted with winter wheat, including Si fertilizers in addition to the conventional reclamation amendments (e.g. cow manure, NPK and lime) in the acid soil polluted by sulphidic mine tailings (collected form the Timok floodplain). The main focus of this study was on the dynamics of P pools in wheat rhizosphere, and on the molecular mechanism of root response to P availability in the rhizosphere (expression of TaPT1 and TaPT2, encoding Pi transporters, TaPEPC encoding PEPC, and MATE-family genes encoding Al-activated citrate efflux transporter). All the amendments induced a significant change in the rhizosphere P fractions (readily available, Al- and Fe-bound P). For instance, Si supply has caused significant increase in readily available P, and reduction in Al-P and Fe-P. This can be attributed to synergetic effect of pH increase and reduction of P sorption by Al- and Fe-oxides. The leaf P concentration in wheat plants treated with Si significantly increased and was in the range of leaf P concentration in P-fertilized plants. While the expressions of the root TaPT2 and TaPEPC were down-regulated by P availability in the rhizosphere and plant P status, TaPT1 and TaMATE showed different pattern with markedly enhanced expression at Si treatment irrespectively of the P supply. In conclusion, Si nutrition effectively alleviates P deficiency in wheat by 1) increased P availability in rhizosphere, most probably due to MATE-mediated citrate exudation, and 2) enhanced P acquisition as a consequence of Si-promoted expressions of PT1 transporter in root plasma membrane.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade",
journal = "1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48",
title = "The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2488"
}

Kostić, L., Samardzic, J., Nikolić, N., Liang, Y.,& Nikolic, M.. (2013). The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings. in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48
Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2488

Kostić L, Samardzic J, Nikolić N, Liang Y, Nikolic M. The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings. in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2488 .

Kostić, Ljiljana, Samardzic, Jelena, Nikolić, Nina, Liang, Yongchao, Nikolic, Miroslav, "The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings" in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48 (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2488 .

TY  - CONF
AU  - Nikolic, Dragana S.
AU  - Nikolic, Dragana B.
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2515
AB  - Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
T1  - Silicon mitigates oxidative stress in cucumber at copper excess
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2515
ER  - 

@conference{
author = "Nikolic, Dragana S. and Nikolic, Dragana B. and Timotijević, Gordana and Pavlović, Jelena and Samardzic, Jelena and Nikolic, Miroslav",
year = "2013",
abstract = "Beneficial effects of silicon (Si) are well demonstrated for many crop species, although its essentiality is SI still not proven. Some mechanisms of Si-mediated alleviation of different stresses in plants have been proposed in the literature, however the molecular bases of these mechanisms are poorly understood. Metal toxicity It, considered as an important environmental problem in many agricultural soils worldwide. Copper (Cu) is an essential microelement for plant growth and development. As a redox-active transition metal, Cu is a cofactor in many proteins involved in electron transfer chains including respiration and photosynthesis. However, in excess Cu can induce oxidative stress via several proposed mechanisms, including direct generation of ro
active oxygen species (ROS) through the Fenton reaction. The aim of th is study was to elucidate the molecular mechanism of Si-mediated alleviation of oxidative stress caused by excess Cu in cucumber. Hydroponically grown plants were subjected to different Cu concentrations, with or without Si supply. The high Cu-treated plants showed higher biomass and better root growth when Si was applied. The parameters of oxidative stress lipid peroxidation, total phenolics and tissue Cu concentrations were measured. Expressions of the genes involved in antioxidative defense and biosynthesis of phenolics were in accordance with the biochemical findings, clearly demonstrating the multiple role of Si in alleviation the harmful effects of ROS in cucumber.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia",
title = "Silicon mitigates oxidative stress in cucumber at copper excess",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2515"
}

Nikolic, D. S., Nikolic, D. B., Timotijević, G., Pavlović, J., Samardzic, J.,& Nikolic, M.. (2013). Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2515

Nikolic DS, Nikolic DB, Timotijević G, Pavlović J, Samardzic J, Nikolic M. Silicon mitigates oxidative stress in cucumber at copper excess. in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .

Nikolic, Dragana S., Nikolic, Dragana B., Timotijević, Gordana, Pavlović, Jelena, Samardzic, Jelena, Nikolic, Miroslav, "Silicon mitigates oxidative stress in cucumber at copper excess" in 1st International Conference on Plant Biology - Programme and Abstracts June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2515 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Ilic, Petar
AU  - Maksimović, Vuk
AU  - Kostić, Ljiljana
AU  - Stevic, Nenad
AU  - Nikolić, Nina
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2450
AB  - Silicon (Si) and iron (Fe) are respectively the second and the forth most abundant
minerals in the earth’s crust. While the essentiality of Fe is discovered at the middle
of the 19th century, Si is still not fully accepted as an essential element for higher
plants. However, Si is the only known element that alleviates multiple stresses in
plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency
chlorosis is a wide-spread nutritional disorder of many crops grown in calcareous and
alkaline soils. The various adaptation mechanisms are involved in Fe acquisition from
rhizosphere by roots of the so-called strategy 1 plants (all dicots and monocot species,
with exception of grasses which belong to strategy 2), i.e. morphological changes (e.g.
lateral roots and enhanced root hair formation in the apical zones) and physiological
changes such as enhanced proton excretion, FeIII reduction by a plasma membrane
reductase and Fe uptake via an inducible FeII transporter (IRT1). These root responses
have been studied and characterized mainly in the nutrient solutions without Si
supply. Therefore, unambiguous information on an interaction between these two
mineral elements is still lacking. We demonstrated for the first time that the
application of Si in nutrient solution experiments also ameliorates Fe deficiency
chlorosis in cucumber, a Si accumulating dicot, which is also commonly used as a
model plant of strategy 1. I will present recent work from our lab in the context of the
effect of Si on both physiological (e.g. FeIII reducing capacity, release of phenolics
and organic acids) and molecular (e.g. expression of FRO2, HA1 and IRT1, the genes
coding FeIII chelate reductase, H+-ATPase and IRT1, respectively) aspects of root
responses to Fe deficiency. In particular, I will focus on the storage and utilization of root apoplastic Fe, root-to-shoot Fe transport and utilization of Fe from the leaf
apoplast. Based on these investigations we propose the possible role of Si in Fe
deficiency stress as 1) increasing apoplastic Fe pool in roots and 2) improving internal
Fe status and thus delaying Fe chlorosis, rather than a direct regulatory/signaling
effect of Si on the key Fe deficiency inducible root responses.
PB  - ISSAG
C3  - Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China
T1  - Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2450
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Ilic, Petar and Maksimović, Vuk and Kostić, Ljiljana and Stevic, Nenad and Nikolić, Nina and Liang, Yongchao and Nikolic, Miroslav",
year = "2011",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the forth most abundant
minerals in the earth’s crust. While the essentiality of Fe is discovered at the middle
of the 19th century, Si is still not fully accepted as an essential element for higher
plants. However, Si is the only known element that alleviates multiple stresses in
plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency
chlorosis is a wide-spread nutritional disorder of many crops grown in calcareous and
alkaline soils. The various adaptation mechanisms are involved in Fe acquisition from
rhizosphere by roots of the so-called strategy 1 plants (all dicots and monocot species,
with exception of grasses which belong to strategy 2), i.e. morphological changes (e.g.
lateral roots and enhanced root hair formation in the apical zones) and physiological
changes such as enhanced proton excretion, FeIII reduction by a plasma membrane
reductase and Fe uptake via an inducible FeII transporter (IRT1). These root responses
have been studied and characterized mainly in the nutrient solutions without Si
supply. Therefore, unambiguous information on an interaction between these two
mineral elements is still lacking. We demonstrated for the first time that the
application of Si in nutrient solution experiments also ameliorates Fe deficiency
chlorosis in cucumber, a Si accumulating dicot, which is also commonly used as a
model plant of strategy 1. I will present recent work from our lab in the context of the
effect of Si on both physiological (e.g. FeIII reducing capacity, release of phenolics
and organic acids) and molecular (e.g. expression of FRO2, HA1 and IRT1, the genes
coding FeIII chelate reductase, H+-ATPase and IRT1, respectively) aspects of root
responses to Fe deficiency. In particular, I will focus on the storage and utilization of root apoplastic Fe, root-to-shoot Fe transport and utilization of Fe from the leaf
apoplast. Based on these investigations we propose the possible role of Si in Fe
deficiency stress as 1) increasing apoplastic Fe pool in roots and 2) improving internal
Fe status and thus delaying Fe chlorosis, rather than a direct regulatory/signaling
effect of Si on the key Fe deficiency inducible root responses.",
publisher = "ISSAG",
journal = "Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China",
title = "Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2450"
}

Pavlović, J., Samardzic, J., Ilic, P., Maksimović, V., Kostić, L., Stevic, N., Nikolić, N., Liang, Y.,& Nikolic, M.. (2011). Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s). in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China
ISSAG..
https://hdl.handle.net/21.15107/rcub_rimsi_2450

Pavlović J, Samardzic J, Ilic P, Maksimović V, Kostić L, Stevic N, Nikolić N, Liang Y, Nikolic M. Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s). in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China. 2011;.
https://hdl.handle.net/21.15107/rcub_rimsi_2450 .

Pavlović, Jelena, Samardzic, Jelena, Ilic, Petar, Maksimović, Vuk, Kostić, Ljiljana, Stevic, Nenad, Nikolić, Nina, Liang, Yongchao, Nikolic, Miroslav, "Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)" in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China (2011),
https://hdl.handle.net/21.15107/rcub_rimsi_2450 .