TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Đorđević Aleksić, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2173
AB  - One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.
PB  - Serbian Society of Toxicology
C3  - 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2173
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić Jeftić, Željka and Subotić, Srđan and Đorđević Aleksić, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2023",
abstract = "One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.",
publisher = "Serbian Society of Toxicology",
journal = "13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2173"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić Jeftić, Ž., Subotić, S., Đorđević Aleksić, J., Lenhardt, M.,& Vuković-Gačić, B.. (2023). Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173

Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić Jeftić Ž, Subotić S, Đorđević Aleksić J, Lenhardt M, Vuković-Gačić B. Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić Jeftić, Željka, Subotić, Srđan, Đorđević Aleksić, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment." in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):72,
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

TY  - JOUR
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Joksimović, Danijela
AU  - Martinović, Rajko
AU  - Ramšak, Andreja
AU  - Bajt, Oliver
AU  - Đorđević Aleksić, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2160
AB  - In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)
EP  - 12
IS  - 38
SP  - 3
VL  - 1
DO  - 10.1093/mutage/geac017
ER  - 

@article{
author = "Vuković-Gačić, Branka and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Joksimović, Danijela and Martinović, Rajko and Ramšak, Andreja and Bajt, Oliver and Đorđević Aleksić, Jelena",
year = "2023",
abstract = "In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)",
pages = "12-3",
number = "38",
volume = "1",
doi = "10.1093/mutage/geac017"
}

Vuković-Gačić, B., Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Joksimović, D., Martinović, R., Ramšak, A., Bajt, O.,& Đorđević Aleksić, J.. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis
Oxford University Press., 1(38), 3-12.
https://doi.org/10.1093/mutage/geac017

Vuković-Gačić B, Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Joksimović D, Martinović R, Ramšak A, Bajt O, Đorđević Aleksić J. Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis. 2023;1(38):3-12.
doi:10.1093/mutage/geac017 .

Vuković-Gačić, Branka, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Joksimović, Danijela, Martinović, Rajko, Ramšak, Andreja, Bajt, Oliver, Đorđević Aleksić, Jelena, "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)" in Mutagenesis, 1, no. 38 (2023):3-12,
https://doi.org/10.1093/mutage/geac017 . .

TY  - THES
AU  - Đorđević Aleksić, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2184
AB  - U ovoj disertaciji su sintetisana jedinjenja sa istom farmakoforom kao i biološki aktivna jedinjenja poreklom iz prirodnih proizvoda, alkiltio i ariltio derivati 2-terc-butil-1,4-benzohinona – TBQ i ispitana je njihova biološka aktivnost.
Jedinjenja pokazuju dobar antioksidativni potencijal i dovode do produkcije slobodnih radikala, pri čemu se najviše ističe 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon (derivat 4), koji zajedno sa 2-terc-butil-5-(propiltio)-1,4-benzohinonom (derivat 3) pokazuje najjači antineurodegenerativni potencijal. Mikrodilucionim testom uočena je jača antimikrobna aktivnost prema gram-pozitivnim bakterijama, naročito prema Staphylococcus aureus i Bacillus subtilis. Svi derivati, osim 2-terc-butil-6-(feniltio)-1,4-benzohinona (derivat 6), pokazuju manju toksičnost od TBQ prema Artemia salina. MTT i MTS testom je uočeno da jedinjenja, generalno, pokazuju umeren citotoksični efekat prema humanim ćelijskim linijama dok je derivat 4 pokazao najjači efekat. Jedinjenja su pokazala jači efekat na inhibiciju migracije tumorske A549 ćelijske linije u odnosu na zdravu MRC-5 ćelijsku liniju. Genotoksična aktivnost jedinjenja primenom SOS/umuC testa na prokariotskom model sistemu nije uočena, niti je primećena interakcija sa plazmidnom DNK. Komet testom, na eukariotskim model sistemima, pokazano je da izabrane hemijske modifikacije pojačavaju genotoksični potencijal dok je derivat 4 pokazao najjači efekat, uključujući indukciju dvolančanih oštećenja DNK γH2AX testom i značajno veće zaustavljanje ćelijskog ciklusa HepG2 ćelija u G2/M fazi u odnosu na TBQ.
Analizom svih dobijenih rezultata, derivat 4 je pokazao najjaču biološku aktivnost.
T2  - Univerzitet u Beogradu, Biološki fakultet
T1  - Biološka aktivnost alkiltio i ariltio derivata 2-terc-butil-1,4-benzohinona
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2184
ER  - 

@phdthesis{
author = "Đorđević Aleksić, Jelena",
year = "2023",
abstract = "U ovoj disertaciji su sintetisana jedinjenja sa istom farmakoforom kao i biološki aktivna jedinjenja poreklom iz prirodnih proizvoda, alkiltio i ariltio derivati 2-terc-butil-1,4-benzohinona – TBQ i ispitana je njihova biološka aktivnost.
Jedinjenja pokazuju dobar antioksidativni potencijal i dovode do produkcije slobodnih radikala, pri čemu se najviše ističe 2-terc-butil-5,6-(etileneditio)-1,4-benzohinon (derivat 4), koji zajedno sa 2-terc-butil-5-(propiltio)-1,4-benzohinonom (derivat 3) pokazuje najjači antineurodegenerativni potencijal. Mikrodilucionim testom uočena je jača antimikrobna aktivnost prema gram-pozitivnim bakterijama, naročito prema Staphylococcus aureus i Bacillus subtilis. Svi derivati, osim 2-terc-butil-6-(feniltio)-1,4-benzohinona (derivat 6), pokazuju manju toksičnost od TBQ prema Artemia salina. MTT i MTS testom je uočeno da jedinjenja, generalno, pokazuju umeren citotoksični efekat prema humanim ćelijskim linijama dok je derivat 4 pokazao najjači efekat. Jedinjenja su pokazala jači efekat na inhibiciju migracije tumorske A549 ćelijske linije u odnosu na zdravu MRC-5 ćelijsku liniju. Genotoksična aktivnost jedinjenja primenom SOS/umuC testa na prokariotskom model sistemu nije uočena, niti je primećena interakcija sa plazmidnom DNK. Komet testom, na eukariotskim model sistemima, pokazano je da izabrane hemijske modifikacije pojačavaju genotoksični potencijal dok je derivat 4 pokazao najjači efekat, uključujući indukciju dvolančanih oštećenja DNK γH2AX testom i značajno veće zaustavljanje ćelijskog ciklusa HepG2 ćelija u G2/M fazi u odnosu na TBQ.
Analizom svih dobijenih rezultata, derivat 4 je pokazao najjaču biološku aktivnost.",
journal = "Univerzitet u Beogradu, Biološki fakultet",
title = "Biološka aktivnost alkiltio i ariltio derivata 2-terc-butil-1,4-benzohinona",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2184"
}

Đorđević Aleksić, J.. (2023). Biološka aktivnost alkiltio i ariltio derivata 2-terc-butil-1,4-benzohinona. in Univerzitet u Beogradu, Biološki fakultet.
https://hdl.handle.net/21.15107/rcub_rimsi_2184

Đorđević Aleksić J. Biološka aktivnost alkiltio i ariltio derivata 2-terc-butil-1,4-benzohinona. in Univerzitet u Beogradu, Biološki fakultet. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2184 .

Đorđević Aleksić, Jelena, "Biološka aktivnost alkiltio i ariltio derivata 2-terc-butil-1,4-benzohinona" in Univerzitet u Beogradu, Biološki fakultet (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2184 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Novaković, Irena
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2174
AB  - 2-tert-butyl-1,4-benzoquinone (TBQ) and its alkylthio and arylthio derivatives: 2-tert-butyl-5-(isopropylthio)-1,4-ben- zoquinone, 2-tert-butyl-5-(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)-1,4-benzoquinone were synthe- sized as analogs of biologically active compounds of natural origin with antitumor activity: quinone avarol/avarone.
The γH2AX test was used to detect the potential genotoxic effect of TBQ and its derivatives in the HepG2 cell line. In addition, the effect of these substances on the cell cycle was monitored on the same HepG2 model system. Since the phosphorylation of histone H2AX is an early event in the cellular response to DNA double-strand breaks (DSBs), an assay based on the detection of phosphorylated histone H2AX (γH2AX) can be used as a biomarker of genotoxicity and genomic instability. In this work, the γH2AX test and flow cytometry were used to analyze the genotoxic potential and the effect on the cell cycle of TBQ and its derivatives.
Double-strand breaks (DSBs) in DNA are detected with γH2AX-specific antibodies, and the cell cycle in the HepG2 cell line is analyzed by flow cytometry. Based on the obtained results, only 2-tert-butyl-5,6- (ethylenedithio)-1,4-benzoquinone induced increased formation of DSBs.
Also, the same derivative caused a significantly greater arrest of cells in the G2/M phase of the cell cycle compared to TBQ from about 27% (TBQ) to 34% of the total population with a decrease in the S phase cell population. By forming DSBs, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone leads to genomic instability of the HepG2 cell line, which results in cell cycle arrest in the G2/M phase.
PB  - Serbian Society of Toxicology
C3  - International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Detection of double-strand breaks in DNA molecules by the γH2AX assay and analysis of the cell cycle after treatment with potential antitumor agents TBQ and its alkylthio and arylthio derivatives.
EP  - 138
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2174
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Novaković, Irena and Žegura, Bojana and Vuković-Gačić, Branka",
year = "2023",
abstract = "2-tert-butyl-1,4-benzoquinone (TBQ) and its alkylthio and arylthio derivatives: 2-tert-butyl-5-(isopropylthio)-1,4-ben- zoquinone, 2-tert-butyl-5-(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)-1,4-benzoquinone were synthe- sized as analogs of biologically active compounds of natural origin with antitumor activity: quinone avarol/avarone.
The γH2AX test was used to detect the potential genotoxic effect of TBQ and its derivatives in the HepG2 cell line. In addition, the effect of these substances on the cell cycle was monitored on the same HepG2 model system. Since the phosphorylation of histone H2AX is an early event in the cellular response to DNA double-strand breaks (DSBs), an assay based on the detection of phosphorylated histone H2AX (γH2AX) can be used as a biomarker of genotoxicity and genomic instability. In this work, the γH2AX test and flow cytometry were used to analyze the genotoxic potential and the effect on the cell cycle of TBQ and its derivatives.
Double-strand breaks (DSBs) in DNA are detected with γH2AX-specific antibodies, and the cell cycle in the HepG2 cell line is analyzed by flow cytometry. Based on the obtained results, only 2-tert-butyl-5,6- (ethylenedithio)-1,4-benzoquinone induced increased formation of DSBs.
Also, the same derivative caused a significantly greater arrest of cells in the G2/M phase of the cell cycle compared to TBQ from about 27% (TBQ) to 34% of the total population with a decrease in the S phase cell population. By forming DSBs, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone leads to genomic instability of the HepG2 cell line, which results in cell cycle arrest in the G2/M phase.",
publisher = "Serbian Society of Toxicology",
journal = "International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Detection of double-strand breaks in DNA molecules by the γH2AX assay and analysis of the cell cycle after treatment with potential antitumor agents TBQ and its alkylthio and arylthio derivatives.",
pages = "138-137",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2174"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Novaković, I., Žegura, B.,& Vuković-Gačić, B.. (2023). Detection of double-strand breaks in DNA molecules by the γH2AX assay and analysis of the cell cycle after treatment with potential antitumor agents TBQ and its alkylthio and arylthio derivatives.. in International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 137-138.
https://hdl.handle.net/21.15107/rcub_rimsi_2174

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Novaković I, Žegura B, Vuković-Gačić B. Detection of double-strand breaks in DNA molecules by the γH2AX assay and analysis of the cell cycle after treatment with potential antitumor agents TBQ and its alkylthio and arylthio derivatives.. in International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:137-138.
https://hdl.handle.net/21.15107/rcub_rimsi_2174 .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Novaković, Irena, Žegura, Bojana, Vuković-Gačić, Branka, "Detection of double-strand breaks in DNA molecules by the γH2AX assay and analysis of the cell cycle after treatment with potential antitumor agents TBQ and its alkylthio and arylthio derivatives." in International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):137-138,
https://hdl.handle.net/21.15107/rcub_rimsi_2174 .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1729
AB  - The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1729
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1729"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):69,
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1725
AB  - Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1725
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1725"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2166
AB  - У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.
PB  - Народна библиотека Србије
C3  - Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
T1  - Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2166
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.",
publisher = "Народна библиотека Србије",
journal = "Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia",
title = "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2166"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
Народна библиотека Србије., 155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka" in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2164
AB  - Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
PB  - Prirodno-matematički fakultet, UNS
C3  - 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
T1  - Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2164
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana",
year = "2022",
abstract = "Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.",
publisher = "Prirodno-matematički fakultet, UNS",
journal = "9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.",
title = "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2164"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K.,& Kostić-Vuković, J.. (2022). Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
Prirodno-matematički fakultet, UNS., 2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J. Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.. 2022;2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava" in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia., 2 (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2165
AB  - Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години због чега је један од императива научне заједнице проналазак ефикаснијих хемиотерапеутика. Више од половине лекова природног је порекла, а будући да океани чине око 70% Земљине површине, марински екосистем је одлична основа за добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из медитеранског сунђера Disidea avara и показују разноврсну биолошку активност попут антимикробне и антитуморске. Као и код већине маринских организама, највећe проблемe за њихову примену представљају слаб принос и цена експлоатације. Решење проблема може бити синтеза једињења сличних по хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ), а синтезом алкилтио и арилтио деривата додатно повећање његове активности. Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични, антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата. Генерално, деривати ТBQ показују јачу биолошку активност. Најјача антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1 фази. Додатно, једињења показују добар антиоксидативни и анттинеуродегенеративни потенцијал.
PB  - National Library of Serbia
C3  - Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
T1  - Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata
SP  - 251
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2165
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Рак је водећи узрок смрти људи широм света са 10 милиона умрлих у 2020. години због чега је један од императива научне заједнице проналазак ефикаснијих хемиотерапеутика. Више од половине лекова природног је порекла, а будући да океани чине око 70% Земљине површине, марински екосистем је одлична основа за добијање нових лекова. Хинони аварол и аварон су редокс пар изолован из медитеранског сунђера Disidea avara и показују разноврсну биолошку активност попут антимикробне и антитуморске. Као и код већине маринских организама, највећe проблемe за њихову примену представљају слаб принос и цена експлоатације. Решење проблема може бити синтеза једињења сличних по хемијској структури и деловању аваролу/аварону попут терц-бутилхинона (ТBQ), а синтезом алкилтио и арилтио деривата додатно повећање његове активности. Упоредно је тестиран антибактеријски, токсични, цитотоксични, генотоксични, антиоксидативни и антинеуродегенеративни потенцијал ТBQ и његових деривата. Генерално, деривати ТBQ показују јачу биолошку активност. Најјача антибактеријска активност уочена је на S. aureus и B. subtilis. Модификације повећавају цитотоксичност и генотоксичност на хуманим ћелијским линијама док дериват 2-терц-бутил-5,6-(етиленедитио)-1,4-бензохинон показује најјачу активност и на основу γH2AX теста доводи до дволанчаних прекида ДНК молекула. Сва испитивана једињења снажно заустављају ћелијски циклус у Г0/Г1 фази. Додатно, једињења показују добар антиоксидативни и анттинеуродегенеративни потенцијал.",
publisher = "National Library of Serbia",
journal = "Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia",
title = "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata",
pages = "251",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2165"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
National Library of Serbia., 251.
https://hdl.handle.net/21.15107/rcub_rimsi_2165

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia. 2022;:251.
https://hdl.handle.net/21.15107/rcub_rimsi_2165 .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Uporedni prikaz biološke aktivnosti potencijalnih antitumorskih agenasa: terc-butilhinona i njegovih alkiltio i ariltio derivata" in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia (2022):251,
https://hdl.handle.net/21.15107/rcub_rimsi_2165 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Oalđe Pavlović, Mariana
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletic-Lausevic, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2180
AB  - BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary approach for health maintenance. In addition to their healing properties and application in folk medicine, Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations (double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%), and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on the degradation of the previously formed biofilm of P. aeruginosa PAO1.
PB  - Federation of European Materials Societies
C3  - FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.
T1  - Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?
SP  - 347
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2180
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Oalđe Pavlović, Mariana and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletic-Lausevic, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary approach for health maintenance. In addition to their healing properties and application in folk medicine, Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations (double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%), and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on the degradation of the previously formed biofilm of P. aeruginosa PAO1.",
publisher = "Federation of European Materials Societies",
journal = "FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.",
title = "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?",
pages = "347",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2180"
}

Đorđević Aleksić, J., Oalđe Pavlović, M., Jovanović Marić, J., Kolarević, S., Duletic-Lausevic, S.,& Vuković-Gačić, B.. (2022). Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.
Federation of European Materials Societies., 347.
https://hdl.handle.net/21.15107/rcub_rimsi_2180

Đorđević Aleksić J, Oalđe Pavlović M, Jovanović Marić J, Kolarević S, Duletic-Lausevic S, Vuković-Gačić B. Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.. 2022;:347.
https://hdl.handle.net/21.15107/rcub_rimsi_2180 .

Đorđević Aleksić, Jelena, Oalđe Pavlović, Mariana, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletic-Lausevic, Sonja, Vuković-Gačić, Branka, "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?" in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia. (2022):347,
https://hdl.handle.net/21.15107/rcub_rimsi_2180 .

TY  - CONF
AU  - Oalđe Pavlović, Mariana
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletic-Lausevic, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2179
AB  - BACKGROUND
The increasing bacterial resistance to antibiotics poses a serious global threat. For that reason, the research into plant-derived antibacterial agents has become progressively more interesting, particularly in regard to their capacity to prevent the delay or inability of wound closure and healing.
OBJECTIVES
The aim of this study was to evaluate i) the antibacterial potential of ethanolic extracts (70% ethanol) of 18 Lamiaceae species against four Gram-negative and three Gram-positive bacterial strains; ii) the effect of these extracts on Pseudomonas aeruginosa PAO1 infection of human lung fibroblasts (MRC-5), and iii) the effect on MRC-5 cell migration.
METHODS
The antibacterial activity was tested by minimal inhibitory concentration (MIC) assay. The effect on bacterial infection of MRC-5 cells was determined using the invasion assay, while the cell migration was assessed with Scratch assay.
RESULTS
The tested Gram-positive bacteria, especially Bacillus subtilis, are more sensitive to the effects of ethanolic extracts than the Gram-negative bacteria. Moreover, the tested extracts significantly inhibited the invasion of Pseudomonas aeruginosa PAO1 during MRC-5 infection, and most of them also displayed promising stimulating potential on MRC-5 migration in a disrupted cell monolayer. Finally, the results of IBR (integrated biomarker response) analysis highlighted Salvia officinalis ethanolic extract as the most active one, which is in accordance with the existing data suggesting that this plant represents an effective antibacterial and wound-healing agent commonly used in traditional medicine. Altogether, these results are expected to foster further studies that will focus on combating bacterial resistance to antibiotics using natural products.
PB  - Federation of European Materials Societies
C3  - FEMS Conference on Microbiology 2022, 30 June – 2 July, Belgrade, Serbia
T1  - Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration
EP  - 800
SP  - 799
VL  - 346
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2179
ER  - 

@conference{
author = "Oalđe Pavlović, Mariana and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletic-Lausevic, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
The increasing bacterial resistance to antibiotics poses a serious global threat. For that reason, the research into plant-derived antibacterial agents has become progressively more interesting, particularly in regard to their capacity to prevent the delay or inability of wound closure and healing.
OBJECTIVES
The aim of this study was to evaluate i) the antibacterial potential of ethanolic extracts (70% ethanol) of 18 Lamiaceae species against four Gram-negative and three Gram-positive bacterial strains; ii) the effect of these extracts on Pseudomonas aeruginosa PAO1 infection of human lung fibroblasts (MRC-5), and iii) the effect on MRC-5 cell migration.
METHODS
The antibacterial activity was tested by minimal inhibitory concentration (MIC) assay. The effect on bacterial infection of MRC-5 cells was determined using the invasion assay, while the cell migration was assessed with Scratch assay.
RESULTS
The tested Gram-positive bacteria, especially Bacillus subtilis, are more sensitive to the effects of ethanolic extracts than the Gram-negative bacteria. Moreover, the tested extracts significantly inhibited the invasion of Pseudomonas aeruginosa PAO1 during MRC-5 infection, and most of them also displayed promising stimulating potential on MRC-5 migration in a disrupted cell monolayer. Finally, the results of IBR (integrated biomarker response) analysis highlighted Salvia officinalis ethanolic extract as the most active one, which is in accordance with the existing data suggesting that this plant represents an effective antibacterial and wound-healing agent commonly used in traditional medicine. Altogether, these results are expected to foster further studies that will focus on combating bacterial resistance to antibiotics using natural products.",
publisher = "Federation of European Materials Societies",
journal = "FEMS Conference on Microbiology 2022, 30 June – 2 July, Belgrade, Serbia",
title = "Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration",
pages = "800-799",
volume = "346",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2179"
}

Oalđe Pavlović, M., Đorđević Aleksić, J., Jovanović Marić, J., Kolarević, S., Duletic-Lausevic, S.,& Vuković-Gačić, B.. (2022). Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration. in FEMS Conference on Microbiology 2022, 30 June – 2 July, Belgrade, Serbia
Federation of European Materials Societies., 346, 799-800.
https://hdl.handle.net/21.15107/rcub_rimsi_2179

Oalđe Pavlović M, Đorđević Aleksić J, Jovanović Marić J, Kolarević S, Duletic-Lausevic S, Vuković-Gačić B. Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration. in FEMS Conference on Microbiology 2022, 30 June – 2 July, Belgrade, Serbia. 2022;346:799-800.
https://hdl.handle.net/21.15107/rcub_rimsi_2179 .

Oalđe Pavlović, Mariana, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletic-Lausevic, Sonja, Vuković-Gačić, Branka, "Ethanolic extracts of Lamiaceae species inhibit the bacterial infection of human lung fibroblasts and stimulate cell migration" in FEMS Conference on Microbiology 2022, 30 June – 2 July, Belgrade, Serbia, 346 (2022):799-800,
https://hdl.handle.net/21.15107/rcub_rimsi_2179 .

TY  - CONF
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Perošević-Bajčeta, Ana
AU  - Joksimović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5726
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2172
AB  - More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.
PB  - Institute of Oceanography and Fisheries
C3  - 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
T1  - Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2172
ER  - 

@conference{
author = "Martinović, Rajko and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Gačić, Zoran and Vuković-Gačić, Branka and Perošević-Bajčeta, Ana and Joksimović, Danijela",
year = "2022",
abstract = "More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.",
publisher = "Institute of Oceanography and Fisheries",
journal = "2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries",
title = "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2172"
}

Martinović, R., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Gačić, Z., Vuković-Gačić, B., Perošević-Bajčeta, A.,& Joksimović, D.. (2022). Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
Institute of Oceanography and Fisheries., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172

Martinović R, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Gačić Z, Vuković-Gačić B, Perošević-Bajčeta A, Joksimović D. Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries. 2022;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

Martinović, Rajko, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Gačić, Zoran, Vuković-Gačić, Branka, Perošević-Bajčeta, Ana, Joksimović, Danijela, "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast" in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries (2022):33,
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1454
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
EP  - 3245
IS  - 7
SP  - 3233
VL  - 12
DO  - 10.1039/d1fo00447f
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpic-Aradski, Ana and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
pages = "3245-3233",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f"
}

Oalde, M., Kolarević, S., Živković, J., Alimpic-Aradski, A., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Marin, P. D., Savikin, K., Vukovic-Gacic, B.,& Duletic-Lausević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Soc Chemistry, Cambridge., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalde M, Kolarević S, Živković J, Alimpic-Aradski A, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Marin PD, Savikin K, Vukovic-Gacic B, Duletic-Lausević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpic-Aradski, Ana, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Duletic-Lausević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1688
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.
PB  - Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany
C3  - 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1688
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.",
publisher = "Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany",
journal = "43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1688"
}

Đorđević Aleksić, J., Kostić-Vuković, J., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany..
https://hdl.handle.net/21.15107/rcub_rimsi_1688

Đorđević Aleksić J, Kostić-Vuković J, Sunjog K, Višnjić Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;.
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

Đorđević Aleksić, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods." in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Makarov, Slobodan
AU  - Đorđević Aleksić, Jelena
AU  - Stevanović, Aleksandra
AU  - Malešević, Anastasija
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2178
AB  - INTRODUCTION:
Different orders within the class Diplopoda possess a variety of chemical compounds in their defense secretions: quinones, phenolics, alcohols, aldehydes, ketones, esters, alkaloids, cyanogenic compounds. Defensive secretions of species from the order Julida are regarded as the most complex within Diplopoda, and they are blends of several classes of chemical com- pounds: quinones, esters and ketones. Numerous biological activities of these secretions have been reported: antimicrobial, antioxidative, antineurodegenerative, cytotoxic and embryotoxic on zebrafish. Besides zebrafish embryos, Artemia salina is one of the common model organisms in toxicity assessment which has not been used for screening of toxicity of millipedes’ defensive secretions.
OBJECTIVES:
The main goal of this study was to examine the toxic effects of defensive secretions of two species from the order Julida [Megaphyllum bosniense (MBO) and M. unilineatum (MUN)] using Artemia salina (ARC test).
METHOD / DESIGN:
Adult individuals of M. bosniense were collected during April and May of 2021 on Mt. Avala, near Belgrade, while adults of M. unilineatum were collected during the same period in the Krnjača, suburb of Belgrade. After the capture, millipedes were kept in plastic boxes containing ground cover from the collecting site. The boxes were regularly sprayed with water to maintain high humidity. Due to the fact that the sample was female-biased, defensive secretions of female specimens were used for further analyses. Excretion of defensive secretions was elicited from glands of mentioned species via mechanical stress in closed glass vials. Secretions collected from both species were dissolved in 10 ml of hexane, concentrated under reduced pressure in a rotary evaporator (Rotavapor R-210, Buchi) at 40°C to a dry residue, and redissolved in 50% dimethylsulphoxide (DMSO). The stock concentration of extracts used in ARC test was 20 mg/mL. Before treatments, eggs of A. salina were incubated for 72h with constant lighting and aeration. For the purposes of the experiment, stage II and III larvae were used (separated by phototaxis in 300 ml of seawater). In a plate with 24 wells, 900 μl of seawater with larvae (10-15 per well) was placed and then 100 μl of tested extracts (range of concentrations 0,1 mg/mL - 0,003125 mg/mL) was added. Potassium dichromate (K2Cr2O7) was used as a positive control and DMSO was used as solvent control. The total number of individuals per well was counted after 24h and 48h, as well as the number of living and dead individuals. These data were used for esti- mation of survival rate and determination of LC50 value. The experiment was done in triplicate.
RESULTS:
Our results show that secretions from both species exhibit a toxic effect on the survival of the chosen model organism, with the MBO extract showing weaker activity in comparison with MUN extract. The LC50 value after 24h was about the same for both species (LC50=73,23 μg/mL for MBO and LC50=68,56 μg/mL for MUN). The LC50 value for MBO after 48h was 47,18 μg/ mL, while LC50 value in the same period for MUN was 29,12 μg/mL. Positive control (LC50= 13,5 μg/mL) showed three times stronger effects in relation to MBO and twice as strong when compared to MUN extract. It has also been shown that the num- ber of surviving individuals decreases with increasing concentration of tested extracts and the increasing incubation time.
CONCLUSIONS:
The defense secretions of both tested millipede species show toxic effects in the ARC test. It is shown that MBO extract has a weaker toxic effect than the MUN extract. This result can be linked with the fact that esters of long-chain fatty acids are dominant compounds in MBO, while MUN is almost exclusively benzoquinone-based. Esters detected in MBO are generally regarded as low-toxic compounds, but with the potential to interact with compounds from other chemical classes. However, as MBO achieved toxic effects and many esters that are detected in MBO are new natural products and their biological potential is unknown, further extensive studies are needed to determine their toxicological potential.
PB  - The International Bioscience Conference (2021; Novi Sad)
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
T1  - Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.
EP  - 54
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2178
ER  - 

@conference{
author = "Milovanović, Jelena and Ilić, Bojan and Makarov, Slobodan and Đorđević Aleksić, Jelena and Stevanović, Aleksandra and Malešević, Anastasija and Vuković-Gačić, Branka",
year = "2021",
abstract = "INTRODUCTION:
Different orders within the class Diplopoda possess a variety of chemical compounds in their defense secretions: quinones, phenolics, alcohols, aldehydes, ketones, esters, alkaloids, cyanogenic compounds. Defensive secretions of species from the order Julida are regarded as the most complex within Diplopoda, and they are blends of several classes of chemical com- pounds: quinones, esters and ketones. Numerous biological activities of these secretions have been reported: antimicrobial, antioxidative, antineurodegenerative, cytotoxic and embryotoxic on zebrafish. Besides zebrafish embryos, Artemia salina is one of the common model organisms in toxicity assessment which has not been used for screening of toxicity of millipedes’ defensive secretions.
OBJECTIVES:
The main goal of this study was to examine the toxic effects of defensive secretions of two species from the order Julida [Megaphyllum bosniense (MBO) and M. unilineatum (MUN)] using Artemia salina (ARC test).
METHOD / DESIGN:
Adult individuals of M. bosniense were collected during April and May of 2021 on Mt. Avala, near Belgrade, while adults of M. unilineatum were collected during the same period in the Krnjača, suburb of Belgrade. After the capture, millipedes were kept in plastic boxes containing ground cover from the collecting site. The boxes were regularly sprayed with water to maintain high humidity. Due to the fact that the sample was female-biased, defensive secretions of female specimens were used for further analyses. Excretion of defensive secretions was elicited from glands of mentioned species via mechanical stress in closed glass vials. Secretions collected from both species were dissolved in 10 ml of hexane, concentrated under reduced pressure in a rotary evaporator (Rotavapor R-210, Buchi) at 40°C to a dry residue, and redissolved in 50% dimethylsulphoxide (DMSO). The stock concentration of extracts used in ARC test was 20 mg/mL. Before treatments, eggs of A. salina were incubated for 72h with constant lighting and aeration. For the purposes of the experiment, stage II and III larvae were used (separated by phototaxis in 300 ml of seawater). In a plate with 24 wells, 900 μl of seawater with larvae (10-15 per well) was placed and then 100 μl of tested extracts (range of concentrations 0,1 mg/mL - 0,003125 mg/mL) was added. Potassium dichromate (K2Cr2O7) was used as a positive control and DMSO was used as solvent control. The total number of individuals per well was counted after 24h and 48h, as well as the number of living and dead individuals. These data were used for esti- mation of survival rate and determination of LC50 value. The experiment was done in triplicate.
RESULTS:
Our results show that secretions from both species exhibit a toxic effect on the survival of the chosen model organism, with the MBO extract showing weaker activity in comparison with MUN extract. The LC50 value after 24h was about the same for both species (LC50=73,23 μg/mL for MBO and LC50=68,56 μg/mL for MUN). The LC50 value for MBO after 48h was 47,18 μg/ mL, while LC50 value in the same period for MUN was 29,12 μg/mL. Positive control (LC50= 13,5 μg/mL) showed three times stronger effects in relation to MBO and twice as strong when compared to MUN extract. It has also been shown that the num- ber of surviving individuals decreases with increasing concentration of tested extracts and the increasing incubation time.
CONCLUSIONS:
The defense secretions of both tested millipede species show toxic effects in the ARC test. It is shown that MBO extract has a weaker toxic effect than the MUN extract. This result can be linked with the fact that esters of long-chain fatty acids are dominant compounds in MBO, while MUN is almost exclusively benzoquinone-based. Esters detected in MBO are generally regarded as low-toxic compounds, but with the potential to interact with compounds from other chemical classes. However, as MBO achieved toxic effects and many esters that are detected in MBO are new natural products and their biological potential is unknown, further extensive studies are needed to determine their toxicological potential.",
publisher = "The International Bioscience Conference (2021; Novi Sad)",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021",
title = "Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.",
pages = "54-53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2178"
}

Milovanović, J., Ilić, B., Makarov, S., Đorđević Aleksić, J., Stevanović, A., Malešević, A.,& Vuković-Gačić, B.. (2021). Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
The International Bioscience Conference (2021; Novi Sad)., 53-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2178

Milovanović J, Ilić B, Makarov S, Đorđević Aleksić J, Stevanović A, Malešević A, Vuković-Gačić B. Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021. 2021;:53-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2178 .

Milovanović, Jelena, Ilić, Bojan, Makarov, Slobodan, Đorđević Aleksić, Jelena, Stevanović, Aleksandra, Malešević, Anastasija, Vuković-Gačić, Branka, "Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina." in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021 (2021):53-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2178 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Stevanović, Aleksandra
AU  - Malešević, Anastasija
AU  - Vuković-Gačić, Branka
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Makarov, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2177
AB  - INTRODUCTION:
In recent years, the emphasis of the scientific community has been placed on the invention of new antimicrobial agents due to the increasing resistance of bacteria to antibiotics. However, serious global health concern is focused on bacterial biofilms, a complex structure of a microbiome made up of colonies of bacteria or individual bacterial cells in a group, attached to a surface. Bacterial biofilms are highly resistant to antimicrobial agents and grow on the surfaces of medical implants such as sutures, catheters, and dental implants. Given that plants and animals are a valuable source of natural biologically active products, they are a good basis for finding new antimicrobial and antibiofilm agents. Bacterial strains of Pseudomonas aerug- inosa PAO1 and Staphylococcus aureus are known for biofilm production and cause opportunistic and chronic infections in humans, some of which are due to biofilm production. Due to their characteristic way of life, millipedes (Diplopoda) are char- acterized by a diverse and complex defense against predators, which includes the secretion of various chemical compounds that are toxic, repellent, or tasteless to predators. Analyzes have shown that millipedes produce chemical compounds such as phenols, alkaloids, quinones, terpenoids, cyanogenic compounds, and fatty acid esters, which showed antimicrobial ac- tivity, among other. Representatives of the order Julida, which are frequent in Republic of Serbia, produce defense secretions that are chemically very complex (the most complex within Diplopoda) and exhibit antimicrobial, antioxidant, and neurode- generative potential, so they represent a good basis for the invention of new antibiofilm agents.
OBJECTIVES:
Objectives are to determine the inhibition of biofilm formation and degradation of the formed biofilm of P. aeruginosa PAO1 and S. aureus by defense secretions of selected millipede species from the family Julidae as well as to determine their anti- microbial activity.
METHOD / DESIGN:
Biofilm formation was quantified by the crystal violet staining method, while antimicrobial activity was examined using the broth dilution minimum inhibitory concentration (MIC) test.
RESULTS:
Defensive secretions of Megaphyllum unilineatum (MUN), Pachyiulus hungaricus (PHU), and Cylindroiulus boleti (CBO) showedantimicrobial activity against S. aureus with MIC values of 0.03, 0.06, and 0.06 mg/mL, respectively. On P. aeruginosa PAO1, defense secretions did not show antimicrobial activity even at the highest tested concentration of 1 mg/mL for MUN while for PHU and CBO the MIC was 1 mg/mL, which is most likely due to the high resistance of this bacterial strain. The antibiofilm effect was observed in all tested defense secretions and was more pronounced against S. aureus than against P. aeruginosa PAO1. The strongest biofilm inhibition of S. aureus was at the highest tested concentrations (2 × MIC) with percentages of inhibition of CBO: 88.6%, PHU: 73.7%, and MUN: 67.2%. Degradation of already formed S. aureus biofilm was shown at lower tested defensive secretions concentrations (MIC/4), about 40% of biofilm degradation for MUN and PHU and about 30% for CBO. The strongest inhibition of P. aeruginosa PAO1 biofilm formation was observed at the highest tested concentrations of defensive secretions, 1 and 0.5 mg/mL for PHU (82 and 54%), and CBO (64.3 and 38.5%) while MUN had the strongest activity at the lowest tested concentration of 0.06 mg/mL (34.3%). All examined defense secretions had similar degradation activity of P. aeruginosa PAO1 biofilm with stronger activity at lower tested concentrations (about 30%). Defensive secretions of MUN and PHU extracted in DMSO solvent showed a stronger antibiofilm effect compared to the same ethanol extracts.
CONCLUSIONS:
The defense secretions of MBO, PHU, and CBO show a good basis for further investigations of their use as antimicrobial agents, especially against S. aureus.
PB  - Joint international PSU-UNS Bioscience Conference
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
T1  - Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus
EP  - 147
SP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2177
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Stevanović, Aleksandra and Malešević, Anastasija and Vuković-Gačić, Branka and Milovanović, Jelena and Ilić, Bojan and Makarov, Slobodan",
year = "2021",
abstract = "INTRODUCTION:
In recent years, the emphasis of the scientific community has been placed on the invention of new antimicrobial agents due to the increasing resistance of bacteria to antibiotics. However, serious global health concern is focused on bacterial biofilms, a complex structure of a microbiome made up of colonies of bacteria or individual bacterial cells in a group, attached to a surface. Bacterial biofilms are highly resistant to antimicrobial agents and grow on the surfaces of medical implants such as sutures, catheters, and dental implants. Given that plants and animals are a valuable source of natural biologically active products, they are a good basis for finding new antimicrobial and antibiofilm agents. Bacterial strains of Pseudomonas aerug- inosa PAO1 and Staphylococcus aureus are known for biofilm production and cause opportunistic and chronic infections in humans, some of which are due to biofilm production. Due to their characteristic way of life, millipedes (Diplopoda) are char- acterized by a diverse and complex defense against predators, which includes the secretion of various chemical compounds that are toxic, repellent, or tasteless to predators. Analyzes have shown that millipedes produce chemical compounds such as phenols, alkaloids, quinones, terpenoids, cyanogenic compounds, and fatty acid esters, which showed antimicrobial ac- tivity, among other. Representatives of the order Julida, which are frequent in Republic of Serbia, produce defense secretions that are chemically very complex (the most complex within Diplopoda) and exhibit antimicrobial, antioxidant, and neurode- generative potential, so they represent a good basis for the invention of new antibiofilm agents.
OBJECTIVES:
Objectives are to determine the inhibition of biofilm formation and degradation of the formed biofilm of P. aeruginosa PAO1 and S. aureus by defense secretions of selected millipede species from the family Julidae as well as to determine their anti- microbial activity.
METHOD / DESIGN:
Biofilm formation was quantified by the crystal violet staining method, while antimicrobial activity was examined using the broth dilution minimum inhibitory concentration (MIC) test.
RESULTS:
Defensive secretions of Megaphyllum unilineatum (MUN), Pachyiulus hungaricus (PHU), and Cylindroiulus boleti (CBO) showedantimicrobial activity against S. aureus with MIC values of 0.03, 0.06, and 0.06 mg/mL, respectively. On P. aeruginosa PAO1, defense secretions did not show antimicrobial activity even at the highest tested concentration of 1 mg/mL for MUN while for PHU and CBO the MIC was 1 mg/mL, which is most likely due to the high resistance of this bacterial strain. The antibiofilm effect was observed in all tested defense secretions and was more pronounced against S. aureus than against P. aeruginosa PAO1. The strongest biofilm inhibition of S. aureus was at the highest tested concentrations (2 × MIC) with percentages of inhibition of CBO: 88.6%, PHU: 73.7%, and MUN: 67.2%. Degradation of already formed S. aureus biofilm was shown at lower tested defensive secretions concentrations (MIC/4), about 40% of biofilm degradation for MUN and PHU and about 30% for CBO. The strongest inhibition of P. aeruginosa PAO1 biofilm formation was observed at the highest tested concentrations of defensive secretions, 1 and 0.5 mg/mL for PHU (82 and 54%), and CBO (64.3 and 38.5%) while MUN had the strongest activity at the lowest tested concentration of 0.06 mg/mL (34.3%). All examined defense secretions had similar degradation activity of P. aeruginosa PAO1 biofilm with stronger activity at lower tested concentrations (about 30%). Defensive secretions of MUN and PHU extracted in DMSO solvent showed a stronger antibiofilm effect compared to the same ethanol extracts.
CONCLUSIONS:
The defense secretions of MBO, PHU, and CBO show a good basis for further investigations of their use as antimicrobial agents, especially against S. aureus.",
publisher = "Joint international PSU-UNS Bioscience Conference",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021",
title = "Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus",
pages = "147-146",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2177"
}

Đorđević Aleksić, J., Stevanović, A., Malešević, A., Vuković-Gačić, B., Milovanović, J., Ilić, B.,& Makarov, S.. (2021). Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
Joint international PSU-UNS Bioscience Conference., 146-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2177

Đorđević Aleksić J, Stevanović A, Malešević A, Vuković-Gačić B, Milovanović J, Ilić B, Makarov S. Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021. 2021;:146-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2177 .

Đorđević Aleksić, Jelena, Stevanović, Aleksandra, Malešević, Anastasija, Vuković-Gačić, Branka, Milovanović, Jelena, Ilić, Bojan, Makarov, Slobodan, "Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus" in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021 (2021):146-147,
https://hdl.handle.net/21.15107/rcub_rimsi_2177 .

TY  - CONF
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Vuković-Gačić, Branka
AU  - Duletic-Lausevic, Sonja
AU  - Marin, Petar D.
AU  - Božić Nedeljković, Biljana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2176
AB  - Recently, cancer research has focused on searching for new and more effective antitumor agents of natural origin that can activate multiple defence mechanisms and selectively damage transformed cells. The goal of this research was to assess different antitumor mechanisms of ethanolic extracts of 18 Lamiaceae species traditionally used in Serbian folk medicine and cuisine, as well as their genotoxic potential towards HCT-116 (colorectal cancer) cells. The viability of treated HCT-116 cells was assessed by MTT assay; the production of reactive oxygen species (ROS) by treated HCT- 116 cells was determined using NBT assay, while their production of nitric oxide (NO) was evaluated using Griess assay. The genotoxic activity of the extracts on HCT-116 cells was tested in Comet assay, using etoposide as a positive control. The results indicated that lavender, basil, and rosemary inhibited the proliferation of these cells, significantly lowering their viability. Moreover, lavender and thyme extracts displayed a significant increase in ROS production, whereas ground- ivy, hyssop, lemon balm, peppermint, basil, rosemary, sage, and winter savory have significantly lowered their production. The results of the Griess assay suggested that lavender, motherwort, peppermint, basil, rosemary, sage, winter savory, ironwort, and thyme have significantly increased the production of NO. Furthermore, Comet assay results pointed out that motherwort, peppermint, basil, oregano, marjoram, winter savory, ironwort, wild thyme, thyme, and mountain germander possess genotoxic potential towards HCT-116 cells, while only basil had genotoxic activity statistically similar to etoposide. The obtained results are in accordance with our previous findings, which indicated that these extracts have antigenotoxic and genoprotective activities towards normal cells. Finally, it can be concluded that these traditionally valued plants might act as potent antitumor agents by modulating the proliferation and production of ROS and NO by cancer cells, as well as by expressing significant genotoxic properties towards cancer cells.
PB  - University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)
C3  - 2nd International UNIFood Conference, September 24-25, Belgrade
T1  - Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2176
ER  - 

@conference{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Vuković-Gačić, Branka and Duletic-Lausevic, Sonja and Marin, Petar D. and Božić Nedeljković, Biljana",
year = "2021",
abstract = "Recently, cancer research has focused on searching for new and more effective antitumor agents of natural origin that can activate multiple defence mechanisms and selectively damage transformed cells. The goal of this research was to assess different antitumor mechanisms of ethanolic extracts of 18 Lamiaceae species traditionally used in Serbian folk medicine and cuisine, as well as their genotoxic potential towards HCT-116 (colorectal cancer) cells. The viability of treated HCT-116 cells was assessed by MTT assay; the production of reactive oxygen species (ROS) by treated HCT- 116 cells was determined using NBT assay, while their production of nitric oxide (NO) was evaluated using Griess assay. The genotoxic activity of the extracts on HCT-116 cells was tested in Comet assay, using etoposide as a positive control. The results indicated that lavender, basil, and rosemary inhibited the proliferation of these cells, significantly lowering their viability. Moreover, lavender and thyme extracts displayed a significant increase in ROS production, whereas ground- ivy, hyssop, lemon balm, peppermint, basil, rosemary, sage, and winter savory have significantly lowered their production. The results of the Griess assay suggested that lavender, motherwort, peppermint, basil, rosemary, sage, winter savory, ironwort, and thyme have significantly increased the production of NO. Furthermore, Comet assay results pointed out that motherwort, peppermint, basil, oregano, marjoram, winter savory, ironwort, wild thyme, thyme, and mountain germander possess genotoxic potential towards HCT-116 cells, while only basil had genotoxic activity statistically similar to etoposide. The obtained results are in accordance with our previous findings, which indicated that these extracts have antigenotoxic and genoprotective activities towards normal cells. Finally, it can be concluded that these traditionally valued plants might act as potent antitumor agents by modulating the proliferation and production of ROS and NO by cancer cells, as well as by expressing significant genotoxic properties towards cancer cells.",
publisher = "University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)",
journal = "2nd International UNIFood Conference, September 24-25, Belgrade",
title = "Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2176"
}

Oalđe Pavlović, M., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Vuković-Gačić, B., Duletic-Lausevic, S., Marin, P. D.,& Božić Nedeljković, B.. (2021). Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.. in 2nd International UNIFood Conference, September 24-25, Belgrade
University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)., 63.
https://hdl.handle.net/21.15107/rcub_rimsi_2176

Oalđe Pavlović M, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Vuković-Gačić B, Duletic-Lausevic S, Marin PD, Božić Nedeljković B. Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.. in 2nd International UNIFood Conference, September 24-25, Belgrade. 2021;:63.
https://hdl.handle.net/21.15107/rcub_rimsi_2176 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Vuković-Gačić, Branka, Duletic-Lausevic, Sonja, Marin, Petar D., Božić Nedeljković, Biljana, "Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine." in 2nd International UNIFood Conference, September 24-25, Belgrade (2021):63,
https://hdl.handle.net/21.15107/rcub_rimsi_2176 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4732
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2175
AB  - Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia
PB  - Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2175
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Vuković-Gačić, Branka and Paunović, Momir",
year = "2021",
abstract = "Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia",
publisher = "Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2175"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Vuković-Gačić, B.,& Paunović, M.. (2021). Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Vuković-Gačić B, Paunović M. Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Vuković-Gačić, Branka, Paunović, Momir, "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme." in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

TY  - JOUR
AU  - Pavlović, Mariana Oalde
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović-Marić, Jovana
AU  - Lunic, Tanja
AU  - Mandic, Marija
AU  - Kracun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Bozić-Nedeljković, Biljana
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1502
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
ER  - 

@article{
author = "Pavlović, Mariana Oalde and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Jovanović-Marić, Jovana and Lunic, Tanja and Mandic, Marija and Kracun-Kolarević, Margareta and Živković, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Bozić-Nedeljković, Biljana and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306"
}

Pavlović, M. O., Kolarević, S., Đorđević Aleksić, J., Jovanović-Marić, J., Lunic, T., Mandic, M., Kracun-Kolarević, M., Živković, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K., Vukovic-Gacic, B., Bozić-Nedeljković, B.,& Duletic-Lausević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112306

Pavlović MO, Kolarević S, Đorđević Aleksić J, Jovanović-Marić J, Lunic T, Mandic M, Kracun-Kolarević M, Živković J, Alimpic-Aradski A, Marin PD, Savikin K, Vukovic-Gacic B, Bozić-Nedeljković B, Duletic-Lausević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112306 .

Pavlović, Mariana Oalde, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Jovanović-Marić, Jovana, Lunic, Tanja, Mandic, Marija, Kracun-Kolarević, Margareta, Živković, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Bozić-Nedeljković, Biljana, Duletic-Lausević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112306 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2171
AB  - Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.
PB  - Narodna biblioteka Srbije
C3  - 49. konferencija o aktuelnim temama korišćenja i zaštite voda
T1  - Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije
EP  - 40
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2171
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.",
publisher = "Narodna biblioteka Srbije",
journal = "49. konferencija o aktuelnim temama korišćenja i zaštite voda",
title = "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije",
pages = "40-37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2171"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda
Narodna biblioteka Srbije., 37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda. 2020;:37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije" in 49. konferencija o aktuelnim temama korišćenja i zaštite voda (2020):37-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Novaković, Irena
AU  - Sladić, Dušan
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2155
AB  - Background: Biologically active compounds, originating from a variety of natural sources: plants, animals and microor- ganisms, have great potential for use as antimicrobial agents. Avarol, a compound originating from the Mediterranean sponge Disidea avara, exhibits a number of biological activities including antimicrobial activity. Considering that, avarol was taken as a model for the synthesis of 2-tert-butyl-1,4-benzoquinone (TBQ) derivatives.
Objectives: TBQ was chosen because of its similar chemical structure with avarol who showed strong biological activity but is more accessible and economical than avarol. By selecting the thiol group, we aimed at combining the action of two strong functional groups of natural origin, quinones and thiols, which both have antimicrobial activity.
Methods: In this work antimicrobial activity of TBQ and its derivatives: 2-tert-butyl-5-(isopropylthio)-1.4-benzoqui- none, 2-tert-butyl-5-(propylthio)-1.4-benzoquinone, 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone, 2-tert-bu- tyl-5-(phenylthio)-1.4-benzoquinone and 2-tert-butyl-6-(phenylthio)-1.4-benzoquinone was evaluated by the MIC mi- crodilution method on 7 different ATCC bacterial strains.
Results: All compounds tested showed stronger antimicrobial activity against Gram positive bacterial strains (Entero- coccus faecalis, Staphylococcus aureus, Bacillus spp.). A strong antimicrobial effect (MIC value less than 100μM) was shown by 2-tert-butyl-5,6- (ethylendithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone deriva- tives and 2-tert-butyl-6-(phenylthio)-1,4-benzoquinone against S.aureus. TBQ, 2-tert-butyl-5-(isopropylthio)-1,4-ben- zoquinone, 2-tert-butyl-5, 6-(ethylendithio)-1,4-benzoquinone and 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone showed strong antimicrobial effect against Bacillus spp. According to our results, chemical modifications of TBQ in- crease its antimicrobial activity while derivative 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone is the best candi- date for further testing.
PB  - Federation of European Microbiological Societies - FEMS
C3  - FEMS Online Conference on Microbiology 2020
T1  - Antimicrobial activity of 2-tert-butyl-1.4-benzoquinone and its selected alkylthio and arylthio derivatives.
SP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2155
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Novaković, Irena and Sladić, Dušan and Vuković-Gačić, Branka",
year = "2020",
abstract = "Background: Biologically active compounds, originating from a variety of natural sources: plants, animals and microor- ganisms, have great potential for use as antimicrobial agents. Avarol, a compound originating from the Mediterranean sponge Disidea avara, exhibits a number of biological activities including antimicrobial activity. Considering that, avarol was taken as a model for the synthesis of 2-tert-butyl-1,4-benzoquinone (TBQ) derivatives.
Objectives: TBQ was chosen because of its similar chemical structure with avarol who showed strong biological activity but is more accessible and economical than avarol. By selecting the thiol group, we aimed at combining the action of two strong functional groups of natural origin, quinones and thiols, which both have antimicrobial activity.
Methods: In this work antimicrobial activity of TBQ and its derivatives: 2-tert-butyl-5-(isopropylthio)-1.4-benzoqui- none, 2-tert-butyl-5-(propylthio)-1.4-benzoquinone, 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone, 2-tert-bu- tyl-5-(phenylthio)-1.4-benzoquinone and 2-tert-butyl-6-(phenylthio)-1.4-benzoquinone was evaluated by the MIC mi- crodilution method on 7 different ATCC bacterial strains.
Results: All compounds tested showed stronger antimicrobial activity against Gram positive bacterial strains (Entero- coccus faecalis, Staphylococcus aureus, Bacillus spp.). A strong antimicrobial effect (MIC value less than 100μM) was shown by 2-tert-butyl-5,6- (ethylendithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone deriva- tives and 2-tert-butyl-6-(phenylthio)-1,4-benzoquinone against S.aureus. TBQ, 2-tert-butyl-5-(isopropylthio)-1,4-ben- zoquinone, 2-tert-butyl-5, 6-(ethylendithio)-1,4-benzoquinone and 2-tert-butyl-5-(phenylthio)-1.4-benzoquinone showed strong antimicrobial effect against Bacillus spp. According to our results, chemical modifications of TBQ in- crease its antimicrobial activity while derivative 2-tert-butyl-5,6-(ethylendithio)-1.4-benzoquinone is the best candi- date for further testing.",
publisher = "Federation of European Microbiological Societies - FEMS",
journal = "FEMS Online Conference on Microbiology 2020",
title = "Antimicrobial activity of 2-tert-butyl-1.4-benzoquinone and its selected alkylthio and arylthio derivatives.",
pages = "223",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2155"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Novaković, I., Sladić, D.,& Vuković-Gačić, B.. (2020). Antimicrobial activity of 2-tert-butyl-1.4-benzoquinone and its selected alkylthio and arylthio derivatives.. in FEMS Online Conference on Microbiology 2020
Federation of European Microbiological Societies - FEMS., 223.
https://hdl.handle.net/21.15107/rcub_rimsi_2155

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Novaković I, Sladić D, Vuković-Gačić B. Antimicrobial activity of 2-tert-butyl-1.4-benzoquinone and its selected alkylthio and arylthio derivatives.. in FEMS Online Conference on Microbiology 2020. 2020;:223.
https://hdl.handle.net/21.15107/rcub_rimsi_2155 .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Novaković, Irena, Sladić, Dušan, Vuković-Gačić, Branka, "Antimicrobial activity of 2-tert-butyl-1.4-benzoquinone and its selected alkylthio and arylthio derivatives." in FEMS Online Conference on Microbiology 2020 (2020):223,
https://hdl.handle.net/21.15107/rcub_rimsi_2155 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Ilić, Marija
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2161
AB  - Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters.
Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program.
Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR.
Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).
PB  - Federation of European Microbiological Societies - FEMS
C3  - FEMS Online Conference on Microbiology 2020
T1  - Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study
SP  - 403
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2161
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Ilić, Marija and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters.
Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program.
Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR.
Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).",
publisher = "Federation of European Microbiological Societies - FEMS",
journal = "FEMS Online Conference on Microbiology 2020",
title = "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study",
pages = "403",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2161"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Ilić, M., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020
Federation of European Microbiological Societies - FEMS., 403.
https://hdl.handle.net/21.15107/rcub_rimsi_2161

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Ilić M, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020. 2020;:403.
https://hdl.handle.net/21.15107/rcub_rimsi_2161 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Ilić, Marija, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study" in FEMS Online Conference on Microbiology 2020 (2020):403,
https://hdl.handle.net/21.15107/rcub_rimsi_2161 .

TY  - JOUR
AU  - Jovanović-Maric, Jovana M.
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Smiljanic, Petar B.
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1369
AB  - This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers
EP  - 229
IS  - 2
SP  - 224
VL  - 105
DO  - 10.1007/s00128-020-02944-4
ER  - 

@article{
author = "Jovanović-Maric, Jovana M. and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Smiljanic, Petar B. and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2020",
abstract = "This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers",
pages = "229-224",
number = "2",
volume = "105",
doi = "10.1007/s00128-020-02944-4"
}

Jovanović-Maric, J. M., Kracun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Smiljanic, P. B., Gačić, Z.,& Vukovic-Gacic, B.. (2020). Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 105(2), 224-229.
https://doi.org/10.1007/s00128-020-02944-4

Jovanović-Maric JM, Kracun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Smiljanic PB, Gačić Z, Vukovic-Gacic B. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology. 2020;105(2):224-229.
doi:10.1007/s00128-020-02944-4 .

Jovanović-Maric, Jovana M., Kracun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Smiljanic, Petar B., Gačić, Zoran, Vukovic-Gacic, Branka, "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers" in Bulletin of Environmental Contamination and Toxicology, 105, no. 2 (2020):224-229,
https://doi.org/10.1007/s00128-020-02944-4 . .