TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Dimitrijević, Milena
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2417
AB  - Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
T1  - Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2417
ER  - 

@conference{
author = "Vojvodić, Snežana and Opačić, Miloš and Stanić, Marina and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia",
title = "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2417"
}

Vojvodić, S., Opačić, M., Stanić, M., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2022). Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417

Vojvodić S, Opačić M, Stanić M, Dimitrijević M, Pittman JK, Spasojević I. Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia. 2022;:133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

Vojvodić, Snežana, Opačić, Miloš, Stanić, Marina, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess" in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia (2022):133-133,
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Jevtović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Lizzul, Alessandro Marco
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1371
AB  - The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana
VL  - 260
DO  - 10.1016/j.chemosphere.2020.127553
ER  - 

@article{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Jevtović, Mima and Bogdanović Pristov, Jelena and Stanić, Marina and Lizzul, Alessandro Marco and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana",
volume = "260",
doi = "10.1016/j.chemosphere.2020.127553"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Jevtović, M., Bogdanović Pristov, J., Stanić, M., Lizzul, A. M., Pittman, J. K.,& Spasojević, I.. (2020). The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 260.
https://doi.org/10.1016/j.chemosphere.2020.127553

Vojvodić S, Danilović Luković J, Zechmann B, Jevtović M, Bogdanović Pristov J, Stanić M, Lizzul AM, Pittman JK, Spasojević I. The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere. 2020;260.
doi:10.1016/j.chemosphere.2020.127553 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Jevtović, Mima, Bogdanović Pristov, Jelena, Stanić, Marina, Lizzul, Alessandro Marco, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana" in Chemosphere, 260 (2020),
https://doi.org/10.1016/j.chemosphere.2020.127553 . .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Vojvodić, Snežana
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2408
AB  - Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron
translucent line inserted between two electron dense lines; the outermost layer is a mature mother
wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the
obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of
TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response
to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae
exposed to 5 Gy was not significantly different than in controls. A similar trend was
observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that
no effects of radiation on biomass, at doses applied here, could be observed.
PB  - Institute for Biological Research “Siniša Stanković” and others contributors
C3  - 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia
T1  - The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study
EP  - 153
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2408
ER  - 

@conference{
author = "Danilović Luković, Jelena and Zechmann, Bernd and Vojvodić, Snežana and Bogdanović Pristov, Jelena and Stanić, Marina and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron
translucent line inserted between two electron dense lines; the outermost layer is a mature mother
wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the
obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of
TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response
to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae
exposed to 5 Gy was not significantly different than in controls. A similar trend was
observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that
no effects of radiation on biomass, at doses applied here, could be observed.",
publisher = "Institute for Biological Research “Siniša Stanković” and others contributors",
journal = "14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia",
title = "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study",
pages = "153-152",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2408"
}

Danilović Luković, J., Zechmann, B., Vojvodić, S., Bogdanović Pristov, J., Stanić, M., Pittman, J. K.,& Spasojević, I.. (2019). The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia
Institute for Biological Research “Siniša Stanković” and others contributors., 152-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2408

Danilović Luković J, Zechmann B, Vojvodić S, Bogdanović Pristov J, Stanić M, Pittman JK, Spasojević I. The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia. 2019;:152-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2408 .

Danilović Luković, Jelena, Zechmann, Bernd, Vojvodić, Snežana, Bogdanović Pristov, Jelena, Stanić, Marina, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study" in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia (2019):152-153,
https://hdl.handle.net/21.15107/rcub_rimsi_2408 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2414
AB  - Microalgae are exposed to ionizing radiation from natural (e.g. radionuclides from the soil) and anthropogenic sources (such as radioactive waste, nuclear power accidents, etc). However, the mechanisms of adaptation to radiation are poorly understood. The aim of our study was to determine the effects of X-ray irradiation on the cell wall of Chlorella sorokiniana. C. sorokiniana (CCAP 211/8K) culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1 for 20 days, reaching stationary phase. Cultures were then exposed to X-rays at doses of 1, 2 and 5 Gy, left under the same conditions for additional 24 h, and analyzed using TEM (postfixation with 1% osmium tetroxide, embedded in resin for soft blocks, and stained with uranyl acetate and lead acetate), and gravimetry. The analysis of TEM micrographs showed that there were no significant changes in the thickness of trilaminar sheath for any of the used doses. However, the diameter of the fibrillar wall was increased in response to irradiation: 105.5 ± 7.5 nm for untreated samples vs. 135.0 ± 9.5 nm (p = 0.015) for microalgae exposed to 1 Gy, and 127.9 ± 7.4 nm (p = 0.036) for exposure to 2 Gy. The thickness of the cell wall in microalgae exposed to 5 Gy (118.6 ± 8.6 nm) was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed. It appears that Chlorella sorokiniana responds promptly to ionizing radiation by fortifying its ‘first line of defense’. The observed changes may be of particular interest for bioremediation, taking into account the capacity of the cell wall to bind water-soluble metals, including radionuclides.
PB  - Taylor & Francis Group
C3  - Journal of Experimental Botany
T1  - Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall
EP  - 166
IS  - Supp 1
SP  - 166
VL  - 54
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Bogdanović Pristov, Jelena and Stanić, Marina and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "Microalgae are exposed to ionizing radiation from natural (e.g. radionuclides from the soil) and anthropogenic sources (such as radioactive waste, nuclear power accidents, etc). However, the mechanisms of adaptation to radiation are poorly understood. The aim of our study was to determine the effects of X-ray irradiation on the cell wall of Chlorella sorokiniana. C. sorokiniana (CCAP 211/8K) culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1 for 20 days, reaching stationary phase. Cultures were then exposed to X-rays at doses of 1, 2 and 5 Gy, left under the same conditions for additional 24 h, and analyzed using TEM (postfixation with 1% osmium tetroxide, embedded in resin for soft blocks, and stained with uranyl acetate and lead acetate), and gravimetry. The analysis of TEM micrographs showed that there were no significant changes in the thickness of trilaminar sheath for any of the used doses. However, the diameter of the fibrillar wall was increased in response to irradiation: 105.5 ± 7.5 nm for untreated samples vs. 135.0 ± 9.5 nm (p = 0.015) for microalgae exposed to 1 Gy, and 127.9 ± 7.4 nm (p = 0.036) for exposure to 2 Gy. The thickness of the cell wall in microalgae exposed to 5 Gy (118.6 ± 8.6 nm) was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed. It appears that Chlorella sorokiniana responds promptly to ionizing radiation by fortifying its ‘first line of defense’. The observed changes may be of particular interest for bioremediation, taking into account the capacity of the cell wall to bind water-soluble metals, including radionuclides.",
publisher = "Taylor & Francis Group",
journal = "Journal of Experimental Botany",
title = "Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall",
pages = "166-166",
number = "Supp 1",
volume = "54",
doi = "10.1080/09670262.2019.1626628"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Bogdanović Pristov, J., Stanić, M., Pittman, J. K.,& Spasojević, I.. (2019). Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall. in Journal of Experimental Botany
Taylor & Francis Group., 54(Supp 1), 166-166.
https://doi.org/10.1080/09670262.2019.1626628

Vojvodić S, Danilović Luković J, Zechmann B, Bogdanović Pristov J, Stanić M, Pittman JK, Spasojević I. Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall. in Journal of Experimental Botany. 2019;54(Supp 1):166-166.
doi:10.1080/09670262.2019.1626628 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Bogdanović Pristov, Jelena, Stanić, Marina, Pittman, Jon K., Spasojević, Ivan, "Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall" in Journal of Experimental Botany, 54, no. Supp 1 (2019):166-166,
https://doi.org/10.1080/09670262.2019.1626628 . .

TY  - CONF
AU  - Romanović, Mima
AU  - Stanić, Marina
AU  - Bogdanović Pristov, Jelena
AU  - Morina, Arian
AU  - Dimitrijević, Milena
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2415
AB  - The impact of ionizing radiation on microalgae represents an important biotechnological and environmental issue. However, it has not been sufficiently investigated. Herein, we analyzed the effects of lowdose X-radiation on the growth, lipid production, and chlorophyll (Chl) and carotenoids content in Chlorella sorokiniana (CCAP 211/8K), which is both, a model and biotechnologically relevant species. C. sorokiniana culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1. X-ray irradiation was applied in the early exponential phase of growth, at different doses (1, 2, 5, 10, 20 Gy) and rates (0.06, 0.24, 0.55 Gy/min). Parameters were monitored for 30 days. The exposure to 2 Gy and 5 Gy had a positive impact on biomass production. Dry weight was significantly higher in treated cultures than controls at days 25 and 30. Total lipid content (according to Nile Red fluorescence assay) was increased at day 30 in cultures exposed to 1 Gy (0.06 Gy/min) and 5 Gy (0.24 Gy/min). Chl content was increased for these doses in the exponential phase of growth. Chl b and carotenoids content was not significantly affected by irradiation. It is noteworthy that higher doses (10 and 20 Gy) had suppressing effects on growth and lipid production. The positive effects of ionizing radiation on biomass and lipid production can be attributed to the phenomenon of radiation hormesis (beneficial effects of low dose radiation on different biological parameters). Radiation hormesis has been shown previously documented on a number of plant species, and can be potentially employed in microalgae industry. On the other hand, microalgae are exposed to increased levels of ionizing irradiation in aquatic systems that are infested with radionuclides either naturally or by anthropogenic activity. Our results may add to the understanding of eco-physiology of
microalgae in such systems.
PB  - Taylor & Francis Group
C3  - European Journal of Phycology
T1  - The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana
EP  - 165
IS  - Supp 1
SP  - 164
VL  - 54
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Romanović, Mima and Stanić, Marina and Bogdanović Pristov, Jelena and Morina, Arian and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "The impact of ionizing radiation on microalgae represents an important biotechnological and environmental issue. However, it has not been sufficiently investigated. Herein, we analyzed the effects of lowdose X-radiation on the growth, lipid production, and chlorophyll (Chl) and carotenoids content in Chlorella sorokiniana (CCAP 211/8K), which is both, a model and biotechnologically relevant species. C. sorokiniana culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1. X-ray irradiation was applied in the early exponential phase of growth, at different doses (1, 2, 5, 10, 20 Gy) and rates (0.06, 0.24, 0.55 Gy/min). Parameters were monitored for 30 days. The exposure to 2 Gy and 5 Gy had a positive impact on biomass production. Dry weight was significantly higher in treated cultures than controls at days 25 and 30. Total lipid content (according to Nile Red fluorescence assay) was increased at day 30 in cultures exposed to 1 Gy (0.06 Gy/min) and 5 Gy (0.24 Gy/min). Chl content was increased for these doses in the exponential phase of growth. Chl b and carotenoids content was not significantly affected by irradiation. It is noteworthy that higher doses (10 and 20 Gy) had suppressing effects on growth and lipid production. The positive effects of ionizing radiation on biomass and lipid production can be attributed to the phenomenon of radiation hormesis (beneficial effects of low dose radiation on different biological parameters). Radiation hormesis has been shown previously documented on a number of plant species, and can be potentially employed in microalgae industry. On the other hand, microalgae are exposed to increased levels of ionizing irradiation in aquatic systems that are infested with radionuclides either naturally or by anthropogenic activity. Our results may add to the understanding of eco-physiology of
microalgae in such systems.",
publisher = "Taylor & Francis Group",
journal = "European Journal of Phycology",
title = "The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana",
pages = "165-164",
number = "Supp 1",
volume = "54",
doi = "10.1080/09670262.2019.1626628"
}

Romanović, M., Stanić, M., Bogdanović Pristov, J., Morina, A., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2019). The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana. in European Journal of Phycology
Taylor & Francis Group., 54(Supp 1), 164-165.
https://doi.org/10.1080/09670262.2019.1626628

Romanović M, Stanić M, Bogdanović Pristov J, Morina A, Dimitrijević M, Pittman JK, Spasojević I. The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana. in European Journal of Phycology. 2019;54(Supp 1):164-165.
doi:10.1080/09670262.2019.1626628 .

Romanović, Mima, Stanić, Marina, Bogdanović Pristov, Jelena, Morina, Arian, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana" in European Journal of Phycology, 54, no. Supp 1 (2019):164-165,
https://doi.org/10.1080/09670262.2019.1626628 . .