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Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
dc.creator | Krizak, Strahinja | |
dc.creator | Nikolić, Ljiljana | |
dc.creator | Stanić, Marina | |
dc.creator | Žižić, Milan | |
dc.creator | Zakrzewska, Joanna | |
dc.creator | Zivić, Miroslav | |
dc.creator | Todorović, Nataša | |
dc.date.accessioned | 2022-04-05T14:56:17Z | |
dc.date.available | 2022-04-05T14:56:17Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0923-2508 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/869 | |
dc.description.abstract | We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage. | en |
dc.publisher | Elsevier, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173040/RS// | |
dc.rights | restrictedAccess | |
dc.source | Research in Microbiology | |
dc.subject | Whole-cell patch-clamp | en |
dc.subject | Voltage-dependent inactivation | en |
dc.subject | Outward rectifier | en |
dc.subject | Filamentous fungi | en |
dc.subject | A-9-C | en |
dc.title | Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 173 | |
dc.citation.issue | 3 | |
dc.citation.other | 166(3): 162-173 | |
dc.citation.rank | M23 | |
dc.citation.spage | 162 | |
dc.citation.volume | 166 | |
dc.identifier.doi | 10.1016/j.resmic.2015.02.004 | |
dc.identifier.pmid | 25701762 | |
dc.identifier.scopus | 2-s2.0-84926407109 | |
dc.identifier.wos | 000353600700005 | |
dc.type.version | publishedVersion |