Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
Samo za registrovane korisnike
2015
Autori
Krizak, StrahinjaNikolić, Ljiljana
Stanić, Marina
Žižić, Milan
Zakrzewska, Joanna
Zivić, Miroslav
Todorović, Nataša
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
Ključne reči:
Whole-cell patch-clamp / Voltage-dependent inactivation / Outward rectifier / Filamentous fungi / A-9-CIzvor:
Research in Microbiology, 2015, 166, 3, 162-173Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
DOI: 10.1016/j.resmic.2015.02.004
ISSN: 0923-2508
PubMed: 25701762
WoS: 000353600700005
Scopus: 2-s2.0-84926407109
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Krizak, Strahinja AU - Nikolić, Ljiljana AU - Stanić, Marina AU - Žižić, Milan AU - Zakrzewska, Joanna AU - Zivić, Miroslav AU - Todorović, Nataša PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/869 AB - We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage. PB - Elsevier, Amsterdam T2 - Research in Microbiology T1 - Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores EP - 173 IS - 3 SP - 162 VL - 166 DO - 10.1016/j.resmic.2015.02.004 ER -
@article{ author = "Krizak, Strahinja and Nikolić, Ljiljana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav and Todorović, Nataša", year = "2015", abstract = "We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.", publisher = "Elsevier, Amsterdam", journal = "Research in Microbiology", title = "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores", pages = "173-162", number = "3", volume = "166", doi = "10.1016/j.resmic.2015.02.004" }
Krizak, S., Nikolić, L., Stanić, M., Žižić, M., Zakrzewska, J., Zivić, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology Elsevier, Amsterdam., 166(3), 162-173. https://doi.org/10.1016/j.resmic.2015.02.004
Krizak S, Nikolić L, Stanić M, Žižić M, Zakrzewska J, Zivić M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173. doi:10.1016/j.resmic.2015.02.004 .
Krizak, Strahinja, Nikolić, Ljiljana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173, https://doi.org/10.1016/j.resmic.2015.02.004 . .