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Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)
dc.creator | Platisa, Jelena | |
dc.creator | Veljović-Jovanović, Sonja | |
dc.creator | Kukavica, Biljana | |
dc.creator | Vinterhalter, Branka | |
dc.creator | Smigocki, Ann | |
dc.creator | Ninković, Slavica | |
dc.date.accessioned | 2022-04-05T14:15:40Z | |
dc.date.available | 2022-04-05T14:15:40Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0176-1617 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/259 | |
dc.description.abstract | Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues. | en |
dc.publisher | Elsevier Gmbh, Urban & Fischer Verlag, Jena | |
dc.rights | restrictedAccess | |
dc.source | Journal of Plant Physiology | |
dc.subject | transformation | en |
dc.subject | superoxide dismutase | en |
dc.subject | peroxidase | en |
dc.subject | embryogenic culture | en |
dc.subject | alfalfa | en |
dc.title | Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.) | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 900 | |
dc.citation.issue | 8 | |
dc.citation.other | 165(8): 895-900 | |
dc.citation.rank | M21 | |
dc.citation.spage | 895 | |
dc.citation.volume | 165 | |
dc.identifier.doi | 10.1016/j.jplph.2007.07.021 | |
dc.identifier.pmid | 17997193 | |
dc.identifier.scopus | 2-s2.0-43049151135 | |
dc.identifier.wos | 000256527300012 | |
dc.type.version | publishedVersion |