Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues.
TY - JOUR
AU - Platisa, Jelena
AU - Veljović-Jovanović, Sonja
AU - Kukavica, Biljana
AU - Vinterhalter, Branka
AU - Smigocki, Ann
AU - Ninković, Slavica
PY - 2008
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/259
AB - Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues.
PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2 - Journal of Plant Physiology
T1 - Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)
EP - 900
IS - 8
SP - 895
VL - 165
DO - 10.1016/j.jplph.2007.07.021
ER -
@article{
author = "Platisa, Jelena and Veljović-Jovanović, Sonja and Kukavica, Biljana and Vinterhalter, Branka and Smigocki, Ann and Ninković, Slavica",
year = "2008",
abstract = "Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)",
pages = "900-895",
number = "8",
volume = "165",
doi = "10.1016/j.jplph.2007.07.021"
}
Platisa, J., Veljović-Jovanović, S., Kukavica, B., Vinterhalter, B., Smigocki, A.,& Ninković, S.. (2008). Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.). in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 165(8), 895-900.
https://doi.org/10.1016/j.jplph.2007.07.021
Platisa J, Veljović-Jovanović S, Kukavica B, Vinterhalter B, Smigocki A, Ninković S. Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.). in Journal of Plant Physiology. 2008;165(8):895-900.
doi:10.1016/j.jplph.2007.07.021 .
Platisa, Jelena, Veljović-Jovanović, Sonja, Kukavica, Biljana, Vinterhalter, Branka, Smigocki, Ann, Ninković, Slavica, "Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)" in Journal of Plant Physiology, 165, no. 8 (2008):895-900,
https://doi.org/10.1016/j.jplph.2007.07.021 . .
