TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Bogdanović Pristov, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2015
AB  - The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.
PB  - Serbian Neuroscience Society, Belgrade
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - Background norepinephrine impacts activity of cortical astrocytes
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2015
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bataveljic, Danijela and Bijelic, Dunja and Milicevic, Katarina and Bogdanović Pristov, Jelena",
year = "2023",
abstract = "The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "Background norepinephrine impacts activity of cortical astrocytes",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2015"
}

Nikolic, L., Bataveljic, D., Bijelic, D., Milicevic, K.,& Bogdanović Pristov, J.. (2023). Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society, Belgrade., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015

Nikolic L, Bataveljic D, Bijelic D, Milicevic K, Bogdanović Pristov J. Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

Nikolic, Ljiljana, Bataveljic, Danijela, Bijelic, Dunja, Milicevic, Katarina, Bogdanović Pristov, Jelena, "Background norepinephrine impacts activity of cortical astrocytes" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):51,
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

TY  - JOUR
AU  - Peric, Mina
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicević, Katarina
AU  - Andjus, Pavle R.
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana M.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1546
AB  - We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Approach for patch-clamping using an upright microscope with z-axis movable stage
DO  - 10.1002/jemt.24066
ER  - 

@article{
author = "Peric, Mina and Bataveljic, Danijela and Bijelic, Dunja and Milicević, Katarina and Andjus, Pavle R. and Bogdanović Pristov, Jelena and Nikolić, Ljiljana M.",
year = "2022",
abstract = "We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Approach for patch-clamping using an upright microscope with z-axis movable stage",
doi = "10.1002/jemt.24066"
}

Peric, M., Bataveljic, D., Bijelic, D., Milicević, K., Andjus, P. R., Bogdanović Pristov, J.,& Nikolić, L. M.. (2022). Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique
Wiley, Hoboken..
https://doi.org/10.1002/jemt.24066

Peric M, Bataveljic D, Bijelic D, Milicević K, Andjus PR, Bogdanović Pristov J, Nikolić LM. Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique. 2022;.
doi:10.1002/jemt.24066 .

Peric, Mina, Bataveljic, Danijela, Bijelic, Dunja, Milicević, Katarina, Andjus, Pavle R., Bogdanović Pristov, Jelena, Nikolić, Ljiljana M., "Approach for patch-clamping using an upright microscope with z-axis movable stage" in Microscopy Research and Technique (2022),
https://doi.org/10.1002/jemt.24066 . .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bijelic, Dunja
AU  - Lazarevic, Milica
AU  - Miljkovic, Djordje
AU  - Bogdanović Pristov, Jelena
AU  - Petkovic, Branka
AU  - Andjus, Pavle
AU  - Momcilovic, Miljana
AU  - Nikolic, Ljiljana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2017
AB  - Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), characterized by focal neurodegenerative and demyelinating lesions. A major contributor to the pathogenic process of MS is the complex interaction between astrocytes and the CNS-infiltrating immune cells (CNS-IIC). The aim of our study is to explore how naïve astrocytes respond to the autoreactive immune cells that invade the CNS. For this reason, CNS-IICs were isolated and purified from spinal cords of rats with experimental autoimmune encephalomyelitis. Ca2+ dynamics was monitored in Fluo-4 labeled naïve astrocytes, isolated from spinal cords of wild type rat pups, following brief bath application of CNS-IIC or peripheral immune cells, with different pharmacological agents. CNS-IICs, and not peripheral immune cells, induced robust elevation of intracellular Ca2+ in naïve astrocytes. We demonstrated that this CNS IIC-induced increase in astrocyte Ca2+ does not depend on the metabotropic glutamate receptors, metabotropic purinergic P2Y1 receptors or TRPA1 channels. Remarkably, further research showed that Ca2+ elevation in astrocytes upon exposure to CNS IICs is due to the activation of ionotropic purinergic P2X7 receptors. Bioluminescence assay showed that immune cell-derived ATP is not a cause of astrocytic P2X7 receptor activation. In fact, we showed that CNS-IICs promoted P2X7 receptor activation and increase in cytosolic Ca2+ in astrocytes by astrocytic hemichannel-dependent ATP release mechanism. Our data suggest that direct contact between astrocytes and CNS IICs induce ATP-dependent Ca2+ changes in astrocytes and points to the new aspect of cell-cell interactions in propagation of neuroinflammatory response in CNS autoimmunity.
PB  - Querétaro, México: Instituto de neurobiologia
C3  - Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia
T1  - Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2017
ER  - 

@conference{
author = "Milicevic, Katarina and Bijelic, Dunja and Lazarevic, Milica and Miljkovic, Djordje and Bogdanović Pristov, Jelena and Petkovic, Branka and Andjus, Pavle and Momcilovic, Miljana and Nikolic, Ljiljana",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), characterized by focal neurodegenerative and demyelinating lesions. A major contributor to the pathogenic process of MS is the complex interaction between astrocytes and the CNS-infiltrating immune cells (CNS-IIC). The aim of our study is to explore how naïve astrocytes respond to the autoreactive immune cells that invade the CNS. For this reason, CNS-IICs were isolated and purified from spinal cords of rats with experimental autoimmune encephalomyelitis. Ca2+ dynamics was monitored in Fluo-4 labeled naïve astrocytes, isolated from spinal cords of wild type rat pups, following brief bath application of CNS-IIC or peripheral immune cells, with different pharmacological agents. CNS-IICs, and not peripheral immune cells, induced robust elevation of intracellular Ca2+ in naïve astrocytes. We demonstrated that this CNS IIC-induced increase in astrocyte Ca2+ does not depend on the metabotropic glutamate receptors, metabotropic purinergic P2Y1 receptors or TRPA1 channels. Remarkably, further research showed that Ca2+ elevation in astrocytes upon exposure to CNS IICs is due to the activation of ionotropic purinergic P2X7 receptors. Bioluminescence assay showed that immune cell-derived ATP is not a cause of astrocytic P2X7 receptor activation. In fact, we showed that CNS-IICs promoted P2X7 receptor activation and increase in cytosolic Ca2+ in astrocytes by astrocytic hemichannel-dependent ATP release mechanism. Our data suggest that direct contact between astrocytes and CNS IICs induce ATP-dependent Ca2+ changes in astrocytes and points to the new aspect of cell-cell interactions in propagation of neuroinflammatory response in CNS autoimmunity.",
publisher = "Querétaro, México: Instituto de neurobiologia",
journal = "Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia",
title = "Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2017"
}

Milicevic, K., Bijelic, D., Lazarevic, M., Miljkovic, D., Bogdanović Pristov, J., Petkovic, B., Andjus, P., Momcilovic, M.,& Nikolic, L.. (2020). Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes. in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia
Querétaro, México: Instituto de neurobiologia., 45.
https://hdl.handle.net/21.15107/rcub_rimsi_2017

Milicevic K, Bijelic D, Lazarevic M, Miljkovic D, Bogdanović Pristov J, Petkovic B, Andjus P, Momcilovic M, Nikolic L. Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes. in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia. 2020;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_2017 .

Milicevic, Katarina, Bijelic, Dunja, Lazarevic, Milica, Miljkovic, Djordje, Bogdanović Pristov, Jelena, Petkovic, Branka, Andjus, Pavle, Momcilovic, Miljana, Nikolic, Ljiljana, "Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes" in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia (2020):45,
https://hdl.handle.net/21.15107/rcub_rimsi_2017 .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Korać Jačić, Jelena
AU  - Bijelic, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2020
AB  - Iron, an essential element for living organisms, participates in a wide range of metabolic processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can vary considerably under different physiological conditions, playing a beneficial role in iron economy and homeostasis or contributing to the generation of reactive oxygen species. It is still not known if bioactivity of low-affinity ligands can be modulated by iron binding. Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter activity of NE. However, how astrocyte activity changes when norepinephrine binds iron remains unknown. We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry, at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects were not observed for the NE- Fe3+ complex. Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.
PB  - COST Action CA15133
C3  - Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
T1  - Iron modulates norepinephrine effect on astrocytes
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2020
ER  - 

@conference{
author = "Nikolic, Ljiljana and Korać Jačić, Jelena and Bijelic, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Iron, an essential element for living organisms, participates in a wide range of metabolic processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can vary considerably under different physiological conditions, playing a beneficial role in iron economy and homeostasis or contributing to the generation of reactive oxygen species. It is still not known if bioactivity of low-affinity ligands can be modulated by iron binding. Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter activity of NE. However, how astrocyte activity changes when norepinephrine binds iron remains unknown. We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry, at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects were not observed for the NE- Fe3+ complex. Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.",
publisher = "COST Action CA15133",
journal = "Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland",
title = "Iron modulates norepinephrine effect on astrocytes",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2020"
}

Nikolic, L., Korać Jačić, J., Bijelic, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
COST Action CA15133..
https://hdl.handle.net/21.15107/rcub_rimsi_2020

Nikolic L, Korać Jačić J, Bijelic D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. 2019;.
https://hdl.handle.net/21.15107/rcub_rimsi_2020 .

Nikolic, Ljiljana, Korać Jačić, Jelena, Bijelic, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland (2019),
https://hdl.handle.net/21.15107/rcub_rimsi_2020 .

TY  - CONF
AU  - Korać Jačić, Jelena
AU  - Nikolic, Ljiljana
AU  - Bijelic, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2016
AB  - Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron. Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE. Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from cultured cortical astrocytes prepared from WT rats. Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance calcium sensitive potassium current in astrocytes. Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our ndings point toward compromised astrocyte functions related to the potassium bu ering when NE action is modified by iron.
PB  - Serbian Neuroscience Society, Belgrade
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia.
T1  - IRON MODULATES NOREPINEPHRINE EFFECT ON ASTROCYTES
SP  - 370
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2016
ER  - 

@conference{
author = "Korać Jačić, Jelena and Nikolic, Ljiljana and Bijelic, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron. Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE. Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from cultured cortical astrocytes prepared from WT rats. Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance calcium sensitive potassium current in astrocytes. Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our ndings point toward compromised astrocyte functions related to the potassium bu ering when NE action is modified by iron.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia.",
title = "IRON MODULATES NOREPINEPHRINE EFFECT ON ASTROCYTES",
pages = "370",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2016"
}

Korać Jačić, J., Nikolic, L., Bijelic, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). IRON MODULATES NOREPINEPHRINE EFFECT ON ASTROCYTES. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia.
Serbian Neuroscience Society, Belgrade., 370.
https://hdl.handle.net/21.15107/rcub_rimsi_2016

Korać Jačić J, Nikolic L, Bijelic D, Spasojević I, Bogdanović Pristov J. IRON MODULATES NOREPINEPHRINE EFFECT ON ASTROCYTES. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia.. 2019;:370.
https://hdl.handle.net/21.15107/rcub_rimsi_2016 .

Korać Jačić, Jelena, Nikolic, Ljiljana, Bijelic, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "IRON MODULATES NOREPINEPHRINE EFFECT ON ASTROCYTES" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. (2019):370,
https://hdl.handle.net/21.15107/rcub_rimsi_2016 .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Momcilovic, Miljana
AU  - Bogdanović Pristov, Jelena
AU  - Petkovic, Branka
AU  - Andjus, Pavle
AU  - Miljkovic, Djordje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2006
AB  - Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.
PB  - Serbian Neuroscience Society, Belgrade
C3  - Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
T1  - ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY
SP  - 295
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2006
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bijelic, Dunja and Milicevic, Katarina and Momcilovic, Miljana and Bogdanović Pristov, Jelena and Petkovic, Branka and Andjus, Pavle and Miljkovic, Djordje",
year = "2019",
abstract = "Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract",
title = "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY",
pages = "295",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2006"
}

Nikolic, L., Bijelic, D., Milicevic, K., Momcilovic, M., Bogdanović Pristov, J., Petkovic, B., Andjus, P.,& Miljkovic, D.. (2019). ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY. in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
Serbian Neuroscience Society, Belgrade., 295.
https://hdl.handle.net/21.15107/rcub_rimsi_2006

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Nikolic, Ljiljana, Bijelic, Dunja, Milicevic, Katarina, Momcilovic, Miljana, Bogdanović Pristov, Jelena, Petkovic, Branka, Andjus, Pavle, Miljkovic, Djordje, "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY" in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract (2019):295,
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