Iron modulates norepinephrine effect on astrocytes
Само за регистроване кориснике
2019
Аутори
Nikolic, LjiljanaKorać Jačić, Jelena
Bijelic, Dunja
Spasojević, Ivan
Bogdanović Pristov, Jelena
Конференцијски прилог (Објављена верзија)
,
COST Action CA15133
Метаподаци
Приказ свих података о документуАпстракт
Iron, an essential element for living organisms, participates in a wide range of metabolic processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can vary considerably under different physiological conditions, playing a beneficial role in iron economy and homeostasis or contributing to the generation of reactive oxygen species. It is still not known if bioactivity of low-affinity ligands can be modulated by iron binding. Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter activity of NE. However, how astrocyte activity changes when norepinephrine binds iron remains unknown. We show, using spectrophotometry that NE and Fe3+ form complex i...n the 1:1 stoichiometry, at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects were not observed for the NE- Fe3+ complex. Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.
Кључне речи:
norepinephrine / iron / astrocyteИзвор:
Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland, 2019Издавач:
- COST Action CA15133
Напомена:
- kategorija M34
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Nikolic, Ljiljana AU - Korać Jačić, Jelena AU - Bijelic, Dunja AU - Spasojević, Ivan AU - Bogdanović Pristov, Jelena PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2020 AB - Iron, an essential element for living organisms, participates in a wide range of metabolic processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can vary considerably under different physiological conditions, playing a beneficial role in iron economy and homeostasis or contributing to the generation of reactive oxygen species. It is still not known if bioactivity of low-affinity ligands can be modulated by iron binding. Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter activity of NE. However, how astrocyte activity changes when norepinephrine binds iron remains unknown. We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry, at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects were not observed for the NE- Fe3+ complex. Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP. PB - COST Action CA15133 C3 - Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland T1 - Iron modulates norepinephrine effect on astrocytes UR - https://hdl.handle.net/21.15107/rcub_rimsi_2020 ER -
@conference{ author = "Nikolic, Ljiljana and Korać Jačić, Jelena and Bijelic, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena", year = "2019", abstract = "Iron, an essential element for living organisms, participates in a wide range of metabolic processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can vary considerably under different physiological conditions, playing a beneficial role in iron economy and homeostasis or contributing to the generation of reactive oxygen species. It is still not known if bioactivity of low-affinity ligands can be modulated by iron binding. Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter activity of NE. However, how astrocyte activity changes when norepinephrine binds iron remains unknown. We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry, at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects were not observed for the NE- Fe3+ complex. Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.", publisher = "COST Action CA15133", journal = "Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland", title = "Iron modulates norepinephrine effect on astrocytes", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2020" }
Nikolic, L., Korać Jačić, J., Bijelic, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland COST Action CA15133.. https://hdl.handle.net/21.15107/rcub_rimsi_2020
Nikolic L, Korać Jačić J, Bijelic D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. 2019;. https://hdl.handle.net/21.15107/rcub_rimsi_2020 .
Nikolic, Ljiljana, Korać Jačić, Jelena, Bijelic, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland (2019), https://hdl.handle.net/21.15107/rcub_rimsi_2020 .