TY  - JOUR
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra Lj.
AU  - Algarra, Manuel
AU  - Lazaro-Martinez, Juan M.
AU  - Rodriguez-Castellon, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana
AU  - Beskoski, Vladimir P.
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1203
AB  - Reports about the influence of cerium-oxide nanoparticles (nCeO(2)) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO(2) with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO(2) were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO(2) on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL(-1) of nCeO(2) on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO(2) was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO(2) had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development
IS  - 11
VL  - 8
DO  - 10.3390/plants8110478
ER  - 

@article{
author = "Milenković, Ivana and Mitrović, Aleksandra Lj. and Algarra, Manuel and Lazaro-Martinez, Juan M. and Rodriguez-Castellon, Enrique and Maksimović, Vuk and Spasić, Slađana and Beskoski, Vladimir P. and Radotić, Ksenija",
year = "2019",
abstract = "Reports about the influence of cerium-oxide nanoparticles (nCeO(2)) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO(2) with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO(2) were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO(2) on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL(-1) of nCeO(2) on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO(2) was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO(2) had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development",
number = "11",
volume = "8",
doi = "10.3390/plants8110478"
}

Milenković, I., Mitrović, A. Lj., Algarra, M., Lazaro-Martinez, J. M., Rodriguez-Castellon, E., Maksimović, V., Spasić, S., Beskoski, V. P.,& Radotić, K.. (2019). Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. in Plants-Basel
MDPI, Basel., 8(11).
https://doi.org/10.3390/plants8110478

Milenković I, Mitrović AL, Algarra M, Lazaro-Martinez JM, Rodriguez-Castellon E, Maksimović V, Spasić S, Beskoski VP, Radotić K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. in Plants-Basel. 2019;8(11).
doi:10.3390/plants8110478 .

Milenković, Ivana, Mitrović, Aleksandra Lj., Algarra, Manuel, Lazaro-Martinez, Juan M., Rodriguez-Castellon, Enrique, Maksimović, Vuk, Spasić, Slađana, Beskoski, Vladimir P., Radotić, Ksenija, "Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development" in Plants-Basel, 8, no. 11 (2019),
https://doi.org/10.3390/plants8110478 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Tesanović, Kristina
AU  - Jakovljević, Dragica
AU  - Kaisarević, Sonja
AU  - Sibul, Filip
AU  - Raseta, Milena
AU  - Karaman, Maja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1277
AB  - The polysaccharide (PSH) extracts from the edible mushroom species Coprinus comatus and Coprinellus truncorum were screened in liquid for their acetylcholinesterase inhibitory (AChE) activity. Both extracts were found to display inhibition of the aforementioned enzyme reaching similar IC50 values of 0.62 +/- 0.07 and 0.61 +/- 0.03 mg/mL, respectively. According to the means of FTIR spectroscopy, these PSH extracts mostly contained beta-glucans. However, the presence of some proteins and polyphenolics as minor ingredients were also detected. Compared with existing literature data for anti-AChE activity of the sugar samples, the findings within this study may be treated as a profound bioactivity. Consequently, this study puts some light on the possible use of the screened macrofungi in the palliative treatment of Alzheimer's disease. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - The polysaccharide extracts from the fungi Coprinus comatus and Coprinellus truncorum do exhibit AChE inhibitory activity
EP  - 754
IS  - 5
SP  - 750
VL  - 33
DO  - 10.1080/14786419.2017.1405417
ER  - 

@article{
author = "Pejin, Boris and Tesanović, Kristina and Jakovljević, Dragica and Kaisarević, Sonja and Sibul, Filip and Raseta, Milena and Karaman, Maja",
year = "2019",
abstract = "The polysaccharide (PSH) extracts from the edible mushroom species Coprinus comatus and Coprinellus truncorum were screened in liquid for their acetylcholinesterase inhibitory (AChE) activity. Both extracts were found to display inhibition of the aforementioned enzyme reaching similar IC50 values of 0.62 +/- 0.07 and 0.61 +/- 0.03 mg/mL, respectively. According to the means of FTIR spectroscopy, these PSH extracts mostly contained beta-glucans. However, the presence of some proteins and polyphenolics as minor ingredients were also detected. Compared with existing literature data for anti-AChE activity of the sugar samples, the findings within this study may be treated as a profound bioactivity. Consequently, this study puts some light on the possible use of the screened macrofungi in the palliative treatment of Alzheimer's disease. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "The polysaccharide extracts from the fungi Coprinus comatus and Coprinellus truncorum do exhibit AChE inhibitory activity",
pages = "754-750",
number = "5",
volume = "33",
doi = "10.1080/14786419.2017.1405417"
}

Pejin, B., Tesanović, K., Jakovljević, D., Kaisarević, S., Sibul, F., Raseta, M.,& Karaman, M.. (2019). The polysaccharide extracts from the fungi Coprinus comatus and Coprinellus truncorum do exhibit AChE inhibitory activity. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 33(5), 750-754.
https://doi.org/10.1080/14786419.2017.1405417

Pejin B, Tesanović K, Jakovljević D, Kaisarević S, Sibul F, Raseta M, Karaman M. The polysaccharide extracts from the fungi Coprinus comatus and Coprinellus truncorum do exhibit AChE inhibitory activity. in Natural Product Research. 2019;33(5):750-754.
doi:10.1080/14786419.2017.1405417 .

Pejin, Boris, Tesanović, Kristina, Jakovljević, Dragica, Kaisarević, Sonja, Sibul, Filip, Raseta, Milena, Karaman, Maja, "The polysaccharide extracts from the fungi Coprinus comatus and Coprinellus truncorum do exhibit AChE inhibitory activity" in Natural Product Research, 33, no. 5 (2019):750-754,
https://doi.org/10.1080/14786419.2017.1405417 . .

TY  - JOUR
AU  - Karaman, Maja
AU  - Janjusević, Ljiljana
AU  - Jakovljević, Dragica
AU  - Sibul, Filip
AU  - Pejin, Boris
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1200
AB  - This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of alpha and ss glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 +/- 0.59 mu g/mL) and high potential for reduction of Fe3+ ions (174.11 +/- 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 mu g), the same extract exhibited a moderate anti-AChE activity (10 mu g) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 +/- 0.31 mu g/mL, 197.68 +/- 9.47 mg eq. AA/g d.w. and 0.1 mu g, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract
EP  - 1526
IS  - 10
SP  - 1522
VL  - 33
DO  - 10.1080/14786419.2017.1422183
ER  - 

@article{
author = "Karaman, Maja and Janjusević, Ljiljana and Jakovljević, Dragica and Sibul, Filip and Pejin, Boris",
year = "2019",
abstract = "This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of alpha and ss glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 +/- 0.59 mu g/mL) and high potential for reduction of Fe3+ ions (174.11 +/- 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 mu g), the same extract exhibited a moderate anti-AChE activity (10 mu g) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 +/- 0.31 mu g/mL, 197.68 +/- 9.47 mg eq. AA/g d.w. and 0.1 mu g, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract",
pages = "1526-1522",
number = "10",
volume = "33",
doi = "10.1080/14786419.2017.1422183"
}

Karaman, M., Janjusević, L., Jakovljević, D., Sibul, F.,& Pejin, B.. (2019). Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 33(10), 1522-1526.
https://doi.org/10.1080/14786419.2017.1422183

Karaman M, Janjusević L, Jakovljević D, Sibul F, Pejin B. Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract. in Natural Product Research. 2019;33(10):1522-1526.
doi:10.1080/14786419.2017.1422183 .

Karaman, Maja, Janjusević, Ljiljana, Jakovljević, Dragica, Sibul, Filip, Pejin, Boris, "Anti-hydroxyl radical activity, redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract" in Natural Product Research, 33, no. 10 (2019):1522-1526,
https://doi.org/10.1080/14786419.2017.1422183 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Matović, Branko
AU  - Prekajski, Marija
AU  - Živković, Ljiljana
AU  - Jakovljević, Dragica
AU  - Gojgic-Cvijović, Gordana
AU  - Beskoski, Vladimir P.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1126
AB  - Cerium oxide (CeO2) nanoparticles (CONPs) are interesting biomaterials with various applications in biomedicine, cosmetics and the pharmaceutical industry, but with limited practical application because of their low stability in aqueous media. The aim of this study was to obtain CONPs with increased stability by coating the particles. Microbial exopolysaccharides (levan, pullulan) and glucose were used to prepare CONPs under different synthesis conditions. Coating was attempted by adding the carbohydrates during (direct coating) or after (subsequent coating) the synthesis of CONPs. The obtained nanoparticles were characterized by X-Ray diffraction analysis, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The suspension stability of the uncoated and coated CONPs in aqueous media was evaluated by measuring the hydrodynamic size, zeta potential and turbidity. The FT-IR spectra revealed the differences between coated CONPs and showed the success of subsequent coating with carbohydrates. Coating with carbohydrates improved the stability the CONP suspension by decreasing the size of aggregated particles. The suspensions of levan- and glucose-coated CONPs had the best stability. In this study, CONPs were prepared using non-toxic materials, which were completely environmentally friendly. The obtained results open new horizons for CONP synthesis, improving their biological applications.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating
EP  - 757
IS  - 6
SP  - 745
VL  - 83
DO  - 10.2298/JSC171205031M
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Matović, Branko and Prekajski, Marija and Živković, Ljiljana and Jakovljević, Dragica and Gojgic-Cvijović, Gordana and Beskoski, Vladimir P.",
year = "2018",
abstract = "Cerium oxide (CeO2) nanoparticles (CONPs) are interesting biomaterials with various applications in biomedicine, cosmetics and the pharmaceutical industry, but with limited practical application because of their low stability in aqueous media. The aim of this study was to obtain CONPs with increased stability by coating the particles. Microbial exopolysaccharides (levan, pullulan) and glucose were used to prepare CONPs under different synthesis conditions. Coating was attempted by adding the carbohydrates during (direct coating) or after (subsequent coating) the synthesis of CONPs. The obtained nanoparticles were characterized by X-Ray diffraction analysis, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The suspension stability of the uncoated and coated CONPs in aqueous media was evaluated by measuring the hydrodynamic size, zeta potential and turbidity. The FT-IR spectra revealed the differences between coated CONPs and showed the success of subsequent coating with carbohydrates. Coating with carbohydrates improved the stability the CONP suspension by decreasing the size of aggregated particles. The suspensions of levan- and glucose-coated CONPs had the best stability. In this study, CONPs were prepared using non-toxic materials, which were completely environmentally friendly. The obtained results open new horizons for CONP synthesis, improving their biological applications.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating",
pages = "757-745",
number = "6",
volume = "83",
doi = "10.2298/JSC171205031M"
}

Milenković, I., Radotić, K., Matović, B., Prekajski, M., Živković, L., Jakovljević, D., Gojgic-Cvijović, G.,& Beskoski, V. P.. (2018). Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 83(6), 745-757.
https://doi.org/10.2298/JSC171205031M

Milenković I, Radotić K, Matović B, Prekajski M, Živković L, Jakovljević D, Gojgic-Cvijović G, Beskoski VP. Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating. in Journal of the Serbian Chemical Society. 2018;83(6):745-757.
doi:10.2298/JSC171205031M .

Milenković, Ivana, Radotić, Ksenija, Matović, Branko, Prekajski, Marija, Živković, Ljiljana, Jakovljević, Dragica, Gojgic-Cvijović, Gordana, Beskoski, Vladimir P., "Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating" in Journal of the Serbian Chemical Society, 83, no. 6 (2018):745-757,
https://doi.org/10.2298/JSC171205031M . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasic, Snežana
AU  - Miletic, Srdjan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1050
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
EP  - 136
SP  - 132
VL  - 100
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasic, Snežana and Miletic, Srdjan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
pages = "136-132",
volume = "100",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasic, S., Miletic, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier Science Bv, Amsterdam., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasic S, Miletic S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasic, Snežana, Miletic, Srdjan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasic, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Djurović, Dijana
AU  - Miletic, Srdjan
AU  - Vrvić, Miroslav M.
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/974
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
EP  - 906
IS  - 6
SP  - 901
VL  - 62
DO  - 10.1097/MPG.0000000000001090
ER  - 

@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasic, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Djurović, Dijana and Miletic, Srdjan and Vrvić, Miroslav M. and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
pages = "906-901",
number = "6",
volume = "62",
doi = "10.1097/MPG.0000000000001090"
}

Marinković, V., Rankovic-Janevski, M., Spasic, S., Nikolić-Kokić, A., Lugonja, N., Djurović, D., Miletic, S., Vrvić, M. M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090

Marinković V, Rankovic-Janevski M, Spasic S, Nikolić-Kokić A, Lugonja N, Djurović D, Miletic S, Vrvić MM, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .

Marinković, Vesna, Rankovic-Janevski, Milica, Spasic, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Djurović, Dijana, Miletic, Srdjan, Vrvić, Miroslav M., Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Spasic, Snežana D
AU  - Vojvodic, Nikola M
AU  - Savić, Slobodan
AU  - Raicević, Savo
AU  - Kovacević, Masa
AU  - Savić, Danijela Z
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/791
AB  - An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .
PB  - Wiley-Blackwell, Hoboken
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy
EP  - E37
IS  - 5
SP  - E34
VL  - 55
DO  - 10.1111/epi.12593
ER  - 

@article{
author = "Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Spasic, Snežana D and Vojvodic, Nikola M and Savić, Slobodan and Raicević, Savo and Kovacević, Masa and Savić, Danijela Z and Spasojević, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy",
pages = "E37-E34",
number = "5",
volume = "55",
doi = "10.1111/epi.12593"
}

Ristic, A. J., Sokic, D., Baščarević, V., Spasic, S. D., Vojvodic, N. M., Savić, S., Raicević, S., Kovacević, M., Savić, D. Z.,& Spasojević, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia
Wiley-Blackwell, Hoboken., 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Baščarević V, Spasic SD, Vojvodic NM, Savić S, Raicević S, Kovacević M, Savić DZ, Spasojević I. Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Spasic, Snežana D, Vojvodic, Nikola M, Savić, Slobodan, Raicević, Savo, Kovacević, Masa, Savić, Danijela Z, Spasojević, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .

TY  - JOUR
AU  - Bajčetić, Milica
AU  - Spasic, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/818
AB  - Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Shock
T1  - Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents
EP  - 184
IS  - 3
SP  - 179
VL  - 42
DO  - 10.1097/SHK.0000000000000198
ER  - 

@article{
author = "Bajčetić, Milica and Spasic, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Shock",
title = "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents",
pages = "184-179",
number = "3",
volume = "42",
doi = "10.1097/SHK.0000000000000198"
}

Bajčetić, M., Spasic, S.,& Spasojević, I.. (2014). Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock
Lippincott Williams & Wilkins, Philadelphia., 42(3), 179-184.
https://doi.org/10.1097/SHK.0000000000000198

Bajčetić M, Spasic S, Spasojević I. Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock. 2014;42(3):179-184.
doi:10.1097/SHK.0000000000000198 .

Bajčetić, Milica, Spasic, Snežana, Spasojević, Ivan, "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents" in Shock, 42, no. 3 (2014):179-184,
https://doi.org/10.1097/SHK.0000000000000198 . .

TY  - JOUR
AU  - Bajčetić, Milica
AU  - Otasević, Biljana
AU  - Bozinovic-Prekajski, Niveska
AU  - Spasic, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/817
AB  - Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Annals of Clinical Biochemistry
T1  - Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation
EP  - 556
IS  - 5
SP  - 550
VL  - 51
DO  - 10.1177/0004563213503317
ER  - 

@article{
author = "Bajčetić, Milica and Otasević, Biljana and Bozinovic-Prekajski, Niveska and Spasic, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Annals of Clinical Biochemistry",
title = "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation",
pages = "556-550",
number = "5",
volume = "51",
doi = "10.1177/0004563213503317"
}

Bajčetić, M., Otasević, B., Bozinovic-Prekajski, N., Spasic, S.,& Spasojević, I.. (2014). Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry
Sage Publications Inc, Thousand Oaks., 51(5), 550-556.
https://doi.org/10.1177/0004563213503317

Bajčetić M, Otasević B, Bozinovic-Prekajski N, Spasic S, Spasojević I. Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry. 2014;51(5):550-556.
doi:10.1177/0004563213503317 .

Bajčetić, Milica, Otasević, Biljana, Bozinovic-Prekajski, Niveska, Spasic, Snežana, Spasojević, Ivan, "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation" in Annals of Clinical Biochemistry, 51, no. 5 (2014):550-556,
https://doi.org/10.1177/0004563213503317 . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Spasic, Snežana D
AU  - Laugier, Olga B
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Oreščanin-Dušić, Zorana
AU  - Vrvić, Miroslav M
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/700
AB  - Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.
PB  - Elsevier Science Inc, New York
T2  - Nutrition
T1  - Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas
EP  - 435
IS  - 2
SP  - 431
VL  - 29
DO  - 10.1016/j.nut.2012.07.018
ER  - 

@article{
author = "Lugonja, Nikoleta and Spasic, Snežana D and Laugier, Olga B and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Oreščanin-Dušić, Zorana and Vrvić, Miroslav M",
year = "2013",
abstract = "Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.",
publisher = "Elsevier Science Inc, New York",
journal = "Nutrition",
title = "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas",
pages = "435-431",
number = "2",
volume = "29",
doi = "10.1016/j.nut.2012.07.018"
}

Lugonja, N., Spasic, S. D., Laugier, O. B., Nikolić-Kokić, A., Spasojević, I., Oreščanin-Dušić, Z.,& Vrvić, M. M.. (2013). Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition
Elsevier Science Inc, New York., 29(2), 431-435.
https://doi.org/10.1016/j.nut.2012.07.018

Lugonja N, Spasic SD, Laugier OB, Nikolić-Kokić A, Spasojević I, Oreščanin-Dušić Z, Vrvić MM. Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition. 2013;29(2):431-435.
doi:10.1016/j.nut.2012.07.018 .

Lugonja, Nikoleta, Spasic, Snežana D, Laugier, Olga B, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Oreščanin-Dušić, Zorana, Vrvić, Miroslav M, "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas" in Nutrition, 29, no. 2 (2013):431-435,
https://doi.org/10.1016/j.nut.2012.07.018 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Obradović, Budimir
AU  - Spasic, Snežana D
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/578
AB  - The present review is aimed at elucidating the neonatal 'sepsis redox cycle' - the cascade of inflammatory and redox events involved in the pathogenesis of sepsis in neonates. While adult and neonatal sepses share some common features, there are some substantial differences: higher mortality rates occur in adult sepsis and worse long-term effects are evident in neonatal sepsis survivors. Such epidemiological data may be explained by the lower ability of IL6 and IL8 to activate NF-kappa B-regulated transcription in neonatal sepsis in comparison to TNF-alpha, which is involved in the mechanisms of adult sepsis. The activation of NF-kappa B in neonatal sepsis is further promoted by hydrogen peroxide and results in mitochondrial dysfunction and energy failure as septic neonates experience decreased O-2 consumption as well as lower heat production and body temperature in comparison to healthy peers. In neonates, specific organs that are still under development are vulnerable to sepsis-provoked stress, which may lead to brain, lung, and heart injury, as well as vision and hearing impairments. In the light of the processes integrated here, it is clear that therapeutic approaches should also target specific steps in the neonatal 'sepsis redox cycle' in addition to the current therapeutic approach that is mainly focused on pathogen eradication.
PB  - Bmc, London
T2  - Critical Care
T1  - Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis
IS  - 3
VL  - 16
DO  - 10.1186/cc11183
ER  - 

@article{
author = "Spasojević, Ivan and Obradović, Budimir and Spasic, Snežana D",
year = "2012",
abstract = "The present review is aimed at elucidating the neonatal 'sepsis redox cycle' - the cascade of inflammatory and redox events involved in the pathogenesis of sepsis in neonates. While adult and neonatal sepses share some common features, there are some substantial differences: higher mortality rates occur in adult sepsis and worse long-term effects are evident in neonatal sepsis survivors. Such epidemiological data may be explained by the lower ability of IL6 and IL8 to activate NF-kappa B-regulated transcription in neonatal sepsis in comparison to TNF-alpha, which is involved in the mechanisms of adult sepsis. The activation of NF-kappa B in neonatal sepsis is further promoted by hydrogen peroxide and results in mitochondrial dysfunction and energy failure as septic neonates experience decreased O-2 consumption as well as lower heat production and body temperature in comparison to healthy peers. In neonates, specific organs that are still under development are vulnerable to sepsis-provoked stress, which may lead to brain, lung, and heart injury, as well as vision and hearing impairments. In the light of the processes integrated here, it is clear that therapeutic approaches should also target specific steps in the neonatal 'sepsis redox cycle' in addition to the current therapeutic approach that is mainly focused on pathogen eradication.",
publisher = "Bmc, London",
journal = "Critical Care",
title = "Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis",
number = "3",
volume = "16",
doi = "10.1186/cc11183"
}

Spasojević, I., Obradović, B.,& Spasic, S. D.. (2012). Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis. in Critical Care
Bmc, London., 16(3).
https://doi.org/10.1186/cc11183

Spasojević I, Obradović B, Spasic SD. Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis. in Critical Care. 2012;16(3).
doi:10.1186/cc11183 .

Spasojević, Ivan, Obradović, Budimir, Spasic, Snežana D, "Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis" in Critical Care, 16, no. 3 (2012),
https://doi.org/10.1186/cc11183 . .

TY  - JOUR
AU  - Andrades, Michael Everton
AU  - Morina, Arian
AU  - Spasic, Snežana D
AU  - Spasojević, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/480
AB  - The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.
PB  - Bmc, London
T2  - Critical Care
T1  - Bench-to-bedside review: Sepsis - from the redox point of view
IS  - 5
VL  - 15
DO  - 10.1186/cc10334
ER  - 

@article{
author = "Andrades, Michael Everton and Morina, Arian and Spasic, Snežana D and Spasojević, Ivan",
year = "2011",
abstract = "The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.",
publisher = "Bmc, London",
journal = "Critical Care",
title = "Bench-to-bedside review: Sepsis - from the redox point of view",
number = "5",
volume = "15",
doi = "10.1186/cc10334"
}

Andrades, M. E., Morina, A., Spasic, S. D.,& Spasojević, I.. (2011). Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care
Bmc, London., 15(5).
https://doi.org/10.1186/cc10334

Andrades ME, Morina A, Spasic SD, Spasojević I. Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care. 2011;15(5).
doi:10.1186/cc10334 .

Andrades, Michael Everton, Morina, Arian, Spasic, Snežana D, Spasojević, Ivan, "Bench-to-bedside review: Sepsis - from the redox point of view" in Critical Care, 15, no. 5 (2011),
https://doi.org/10.1186/cc10334 . .