TY  - JOUR
AU  - Žižić, Milan
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Živić, Miroslav
AU  - Stanić, Marina
AU  - Maksimović, Vuk
AU  - Zakrzewska, Joanna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3165
AB  - BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Biology and Medicine
T1  - Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass
EP  - 127386
SP  - 127381
VL  - 83
DO  - 10.1016/j.jtemb.2024.127381
ER  - 

@article{
author = "Žižić, Milan and Atlagić, Kristina and Karaman, Maja and Živić, Miroslav and Stanić, Marina and Maksimović, Vuk and Zakrzewska, Joanna",
year = "2024",
abstract = "BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Biology and Medicine",
title = "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass",
pages = "127386-127381",
volume = "83",
doi = "10.1016/j.jtemb.2024.127381"
}

Žižić, M., Atlagić, K., Karaman, M., Živić, M., Stanić, M., Maksimović, V.,& Zakrzewska, J.. (2024). Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine
Elsevier Gmbh, Munich., 83, 127381-127386.
https://doi.org/10.1016/j.jtemb.2024.127381

Žižić M, Atlagić K, Karaman M, Živić M, Stanić M, Maksimović V, Zakrzewska J. Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine. 2024;83:127381-127386.
doi:10.1016/j.jtemb.2024.127381 .

Žižić, Milan, Atlagić, Kristina, Karaman, Maja, Živić, Miroslav, Stanić, Marina, Maksimović, Vuk, Zakrzewska, Joanna, "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass" in Journal of Trace Elements in Biology and Medicine, 83 (2024):127381-127386,
https://doi.org/10.1016/j.jtemb.2024.127381 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2216
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.
PB  - Springer Nature
T2  - Springer Nature, Ecotoxicology 32, 583–597 (2023)
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
EP  - 597
IS  - 32
SP  - 583
DO  - https://doi.org/10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.",
publisher = "Springer Nature",
journal = "Springer Nature, Ecotoxicology 32, 583–597 (2023)",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
pages = "597-583",
number = "32",
doi = "https://doi.org/10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023)
Springer Nature.(32), 583-597.
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023). 2023;(32):583-597.
doi:https://doi.org/10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Springer Nature, Ecotoxicology 32, 583–597 (2023), no. 32 (2023):583-597,
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - JOUR
AU  - Dominguez, Jonah
AU  - Raković, Marko
AU  - Li, Donglai
AU  - Pollock, Henry
AU  - Lawson, Shelby
AU  - Novčić, Ivana
AU  - Su, Xiangting
AU  - Zeng, Qisha
AU  - Al-Dhufari, Roqaya
AU  - Johnson-Cadle, Shanelle
AU  - Boldrick, Julia
AU  - Chamberlain, Mac
AU  - Hauber, Mark
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3074
AB  - Alarm signals have evolved to communicate pertinent threats to conspecifics,
but heterospecifics may also use alarm calls to obtain social information. In
birds, mixed-species flocks are often structured around focal sentinel species,
which produce reliable alarm calls that inform eavesdropping heterospecifics
about predation risk. Prior research has shown that Neotropical species
innately recognize the alarm calls of a Nearctic sentinel species, but it remains
unclear how generalizable or consistent such innate signal recognition of
alarm-calling species is. We tested for the responses to the alarm calls
of a Neotropical sentinel forest bird species, the dusky-throated antshrike
(Thamnomanes ardesiacus), by naive resident temperate forest birds across
three continents during the winter season. At all three sites, we found that
approaches to the Neotropical antshrike alarm calls were similarly frequent
to the alarm calls of a local parid sentinel species (positive control), while
approaches to the antshrike’s songs and to non-threatening columbid calls
(negative controls) occurred significantly less often. Although we only
tested one sentinel species, our findings indicate that temperate forest birds
can recognize and adaptively respond globally to a foreign and unfamiliar tropical
alarm call, and suggest that some avian alarm calls transcend
phylogenetic histories and individual ecological experiences.
PB  - The Royal Society
T2  - Biology letters
T1  - What’s the rumpus? Resident temperate forest birds approach an unfamiliar neotropical alarm call across three continents
SP  - 20230332
VL  - 19
DO  - https://doi.org/10.1098/rsbl.2023.0332
ER  - 

@article{
author = "Dominguez, Jonah and Raković, Marko and Li, Donglai and Pollock, Henry and Lawson, Shelby and Novčić, Ivana and Su, Xiangting and Zeng, Qisha and Al-Dhufari, Roqaya and Johnson-Cadle, Shanelle and Boldrick, Julia and Chamberlain, Mac and Hauber, Mark",
year = "2023",
abstract = "Alarm signals have evolved to communicate pertinent threats to conspecifics,
but heterospecifics may also use alarm calls to obtain social information. In
birds, mixed-species flocks are often structured around focal sentinel species,
which produce reliable alarm calls that inform eavesdropping heterospecifics
about predation risk. Prior research has shown that Neotropical species
innately recognize the alarm calls of a Nearctic sentinel species, but it remains
unclear how generalizable or consistent such innate signal recognition of
alarm-calling species is. We tested for the responses to the alarm calls
of a Neotropical sentinel forest bird species, the dusky-throated antshrike
(Thamnomanes ardesiacus), by naive resident temperate forest birds across
three continents during the winter season. At all three sites, we found that
approaches to the Neotropical antshrike alarm calls were similarly frequent
to the alarm calls of a local parid sentinel species (positive control), while
approaches to the antshrike’s songs and to non-threatening columbid calls
(negative controls) occurred significantly less often. Although we only
tested one sentinel species, our findings indicate that temperate forest birds
can recognize and adaptively respond globally to a foreign and unfamiliar tropical
alarm call, and suggest that some avian alarm calls transcend
phylogenetic histories and individual ecological experiences.",
publisher = "The Royal Society",
journal = "Biology letters",
title = "What’s the rumpus? Resident temperate forest birds approach an unfamiliar neotropical alarm call across three continents",
pages = "20230332",
volume = "19",
doi = "https://doi.org/10.1098/rsbl.2023.0332"
}

Dominguez, J., Raković, M., Li, D., Pollock, H., Lawson, S., Novčić, I., Su, X., Zeng, Q., Al-Dhufari, R., Johnson-Cadle, S., Boldrick, J., Chamberlain, M.,& Hauber, M.. (2023). What’s the rumpus? Resident temperate forest birds approach an unfamiliar neotropical alarm call across three continents. in Biology letters
The Royal Society., 19, 20230332.
https://doi.org/https://doi.org/10.1098/rsbl.2023.0332

Dominguez J, Raković M, Li D, Pollock H, Lawson S, Novčić I, Su X, Zeng Q, Al-Dhufari R, Johnson-Cadle S, Boldrick J, Chamberlain M, Hauber M. What’s the rumpus? Resident temperate forest birds approach an unfamiliar neotropical alarm call across three continents. in Biology letters. 2023;19:20230332.
doi:https://doi.org/10.1098/rsbl.2023.0332 .

Dominguez, Jonah, Raković, Marko, Li, Donglai, Pollock, Henry, Lawson, Shelby, Novčić, Ivana, Su, Xiangting, Zeng, Qisha, Al-Dhufari, Roqaya, Johnson-Cadle, Shanelle, Boldrick, Julia, Chamberlain, Mac, Hauber, Mark, "What’s the rumpus? Resident temperate forest birds approach an unfamiliar neotropical alarm call across three continents" in Biology letters, 19 (2023):20230332,
https://doi.org/https://doi.org/10.1098/rsbl.2023.0332 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2115
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/GENSR230159I
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/GENSR230159I"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/GENSR230159I

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA. in Genetika. 2023;55(1):159-176.
doi:10.2298/GENSR230159I .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "CHARACTERIZATION OF Pseudomonas syringae pv. morsprunorum ORIGINATING FROM SWEET CHERRY AND PLUM IN SERBIA" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/GENSR230159I . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Medić, Olja
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2116
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier Inc.
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
EP  - 13
SP  - 105238
SP  - 1
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Medić, Olja and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier Inc.",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "13-105238-1",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Iličić, R., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier Inc.., 182, 105238-13.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Iličić R, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238-13.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Medić, Olja, Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238-13,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2117
AB  - The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Žečević, Katarina
AU  - Stanisavljević, Rade
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2118
AB  - The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.
PB  - British Society for Plant Pathology
PB  - Wiley
T2  - Plant Pathology
T1  - Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans
EP  - 299
IS  - 2
SP  - 290
VL  - 72
DO  - 10.1111/ppa.13658
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Milovanović, Predrag and Stanković, Slaviša and Žečević, Katarina and Stanisavljević, Rade and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.",
publisher = "British Society for Plant Pathology, Wiley",
journal = "Plant Pathology",
title = "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans",
pages = "299-290",
number = "2",
volume = "72",
doi = "10.1111/ppa.13658"
}

Iličić, R., Jelušić, A., Milovanović, P., Stanković, S., Žečević, K., Stanisavljević, R.,& Popović Milovanović, T.. (2023). Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology
British Society for Plant Pathology., 72(2), 290-299.
https://doi.org/10.1111/ppa.13658

Iličić R, Jelušić A, Milovanović P, Stanković S, Žečević K, Stanisavljević R, Popović Milovanović T. Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in Plant Pathology. 2023;72(2):290-299.
doi:10.1111/ppa.13658 .

Iličić, Renata, Jelušić, Aleksandra, Milovanović, Predrag, Stanković, Slaviša, Žečević, Katarina, Stanisavljević, Rade, Popović Milovanović, Tatjana, "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans" in Plant Pathology, 72, no. 2 (2023):290-299,
https://doi.org/10.1111/ppa.13658 . .

TY  - CONF
AU  - Vitkovac, Aleksandra
AU  - Pajić, Tanja
AU  - Bukumira, Marta
AU  - Stanić, Marina
AU  - Rabasovic, Mihailo
AU  - Todorović, Nataša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2422
AB  - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023
C3  - 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
T1  - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
EP  - 337
SP  - 334
DO  - 10.46793/ICCBI23.334V
ER  - 

@conference{
author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasovic, Mihailo and Todorović, Nataša",
year = "2023",
abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023",
journal = "2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023",
title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology",
pages = "337-334",
doi = "10.46793/ICCBI23.334V"
}

Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasovic, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023., 334-337.
https://doi.org/10.46793/ICCBI23.334V

Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasovic M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023. 2023;:334-337.
doi:10.46793/ICCBI23.334V .

Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasovic, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 (2023):334-337,
https://doi.org/10.46793/ICCBI23.334V . .

TY  - JOUR
AU  - Radonjić, Marina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Mrdak, Danilo
AU  - Cirovic, Dusko
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2622
AB  - The aim of this study was to determine the concentrations of 28 elements in the liver, kidney, muscle, and heart of a small Indian mongoose from Montenegro. Element concentrations were determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Significant differences in elemental concentrations between analyzed tissues were observed for Ag, Al, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, P, S, Se, and Zn. The concentrations of Ag, Fe, Mo, and S were significantly different between all analyzed tissues. Muscle tissue is characterised by higher concentrations of some macroelements (K, Mg, S) and liver tissue by higher concentrations of some trace elements (Fe, Mn, Mo, and Zn). Cardiac tissue could be the target organ for bioaccumulation of Ag and Se. The molar ratio Se:Hg was higher than 1 in all studied tissues. There were few significant differences between element concentrations in mongooses from three analyzed (Airport, Saline, Inland) groups. Significantly higher concentrations of Ag and Se were found in liver tissue of Inland mongooses compared to animals from Airport and Saline. There were few significant differences (Zn in liver and muscle, Fe in muscle) in element concentrations between females and males.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring
EP  - 60523
IS  - 21
SP  - 60514
VL  - 30
DO  - 10.1007/s11356-023-26885-7
ER  - 

@article{
author = "Radonjić, Marina and Subotić, Srđan and Višnjić Jeftić, Željka and Mrdak, Danilo and Cirovic, Dusko",
year = "2023",
abstract = "The aim of this study was to determine the concentrations of 28 elements in the liver, kidney, muscle, and heart of a small Indian mongoose from Montenegro. Element concentrations were determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Significant differences in elemental concentrations between analyzed tissues were observed for Ag, Al, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, P, S, Se, and Zn. The concentrations of Ag, Fe, Mo, and S were significantly different between all analyzed tissues. Muscle tissue is characterised by higher concentrations of some macroelements (K, Mg, S) and liver tissue by higher concentrations of some trace elements (Fe, Mn, Mo, and Zn). Cardiac tissue could be the target organ for bioaccumulation of Ag and Se. The molar ratio Se:Hg was higher than 1 in all studied tissues. There were few significant differences between element concentrations in mongooses from three analyzed (Airport, Saline, Inland) groups. Significantly higher concentrations of Ag and Se were found in liver tissue of Inland mongooses compared to animals from Airport and Saline. There were few significant differences (Zn in liver and muscle, Fe in muscle) in element concentrations between females and males.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring",
pages = "60523-60514",
number = "21",
volume = "30",
doi = "10.1007/s11356-023-26885-7"
}

Radonjić, M., Subotić, S., Višnjić Jeftić, Ž., Mrdak, D.,& Cirovic, D.. (2023). Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring. in Environmental Science and Pollution Research
Springer., 30(21), 60514-60523.
https://doi.org/10.1007/s11356-023-26885-7

Radonjić M, Subotić S, Višnjić Jeftić Ž, Mrdak D, Cirovic D. Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring. in Environmental Science and Pollution Research. 2023;30(21):60514-60523.
doi:10.1007/s11356-023-26885-7 .

Radonjić, Marina, Subotić, Srđan, Višnjić Jeftić, Željka, Mrdak, Danilo, Cirovic, Dusko, "Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring" in Environmental Science and Pollution Research, 30, no. 21 (2023):60514-60523,
https://doi.org/10.1007/s11356-023-26885-7 . .

TY  - JOUR
AU  - Jovičić, Katarina
AU  - Janković, Saša
AU  - Nikolić, Dragica
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
AU  - Jarić, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2559
AB  - The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As.
The results suggest that scales and fins have a potential to be used as indicators of muscle tissue
contamination with As, Cu, and Hg, depending on species’ ecological traits.
PB  - EDP Sciences
T2  - Knowledge & Management of Aquatic Ecosystems
T1  - Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River
DO  - https://doi.org/10.1051/kmae/2022027
ER  - 

@article{
author = "Jovičić, Katarina and Janković, Saša and Nikolić, Dragica and Đikanović, Vesna and Skorić, Stefan and Krpo-Ćetković, Jasmina and Jarić, Ivan",
year = "2023",
abstract = "The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As.
The results suggest that scales and fins have a potential to be used as indicators of muscle tissue
contamination with As, Cu, and Hg, depending on species’ ecological traits.",
publisher = "EDP Sciences",
journal = "Knowledge & Management of Aquatic Ecosystems",
title = "Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River",
doi = "https://doi.org/10.1051/kmae/2022027"
}

Jovičić, K., Janković, S., Nikolić, D., Đikanović, V., Skorić, S., Krpo-Ćetković, J.,& Jarić, I.. (2023). Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River. in Knowledge & Management of Aquatic Ecosystems
EDP Sciences..
https://doi.org/https://doi.org/10.1051/kmae/2022027

Jovičić K, Janković S, Nikolić D, Đikanović V, Skorić S, Krpo-Ćetković J, Jarić I. Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River. in Knowledge & Management of Aquatic Ecosystems. 2023;.
doi:https://doi.org/10.1051/kmae/2022027 .

Jovičić, Katarina, Janković, Saša, Nikolić, Dragica, Đikanović, Vesna, Skorić, Stefan, Krpo-Ćetković, Jasmina, Jarić, Ivan, "Prospects of fish scale and fin samples usage for nonlethal 3 monitoring of metal contamination: a study on five fish species 4 from the Danube River" in Knowledge & Management of Aquatic Ecosystems (2023),
https://doi.org/https://doi.org/10.1051/kmae/2022027 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1919
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bataveljic, Danijela
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Bogdanović Pristov, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2015
AB  - The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.
PB  - Serbian Neuroscience Society, Belgrade
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - Background norepinephrine impacts activity of cortical astrocytes
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2015
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bataveljic, Danijela and Bijelic, Dunja and Milicevic, Katarina and Bogdanović Pristov, Jelena",
year = "2023",
abstract = "The neurotransmitter norepinephrine (NE) plays a central role in regulating arousal, 
attention, cognitive function and stress responses. Unlike fast neurotransmitters which 
act at synapses, NE is released in the neuropil and performs multiple targeting in the 
surrounding area. The glial cells astrocytes are a direct target of NE, as they express 
all adrenergic receptor subtypes and respond with Ca2+ increases to NE. Astroglial 
responses elicited by strong and transient increases of NE in the brain are well studied, 
but the effect of a low background NE concentration on astrocytes is unknown. This 
background level of NE is maintained by basal noradrenergic activity and is 
constantly present in the brain. Therefore, the response of astrocytes to the 
background NE could have been unintentionally evoked in previous studies but its 
effect overlooked. 
To assess action of background NE on astrocytes we combined the whole-cell patch
clamp, immunohistochemistry, Ca2+ imaging and pharmacology. We used cultured 
cortical astrocytes to bypass NE targeting of multiple cell types. 
We show that cortical astrocytes detect and respond to the background NE 
concentration with an increase in intracellular Ca2+. This Ca2+ liberated from 
intracellular stores further increased large-conductance, Ca2+-sensitive potassium 
(BK) currents in astrocytes. Notably, immunohistochemistry data showed that BK 
channels and alpha 1 adrenoreceptor are highly expressed in astrocytes in the rat 
cortex.   Furthermore, stimulation of astrocytes by background NE was inhibited by 
alpha-adrenoceptor antagonist. 
Our results suggest that astrocytes maintain basal brain activity by perceiving and 
responding to the background NE.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "Background norepinephrine impacts activity of cortical astrocytes",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2015"
}

Nikolic, L., Bataveljic, D., Bijelic, D., Milicevic, K.,& Bogdanović Pristov, J.. (2023). Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society, Belgrade., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015

Nikolic L, Bataveljic D, Bijelic D, Milicevic K, Bogdanović Pristov J. Background norepinephrine impacts activity of cortical astrocytes. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

Nikolic, Ljiljana, Bataveljic, Danijela, Bijelic, Dunja, Milicevic, Katarina, Bogdanović Pristov, Jelena, "Background norepinephrine impacts activity of cortical astrocytes" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):51,
https://hdl.handle.net/21.15107/rcub_rimsi_2015 .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2009
AB  - Astrocytes form a dense meshwork throughout the central nervous system (CNS) which qualifies them to perform interactive maintenance functions with neighboring cells. In neuroinflammation, this astroglial cell-to-cell interaction varies which can either promote or lessen pathological processes (1,2). In multiple sclerosis (MS), astrocytes engage in an interaction with immune cells which drives neurodegeneration by creating and sustaining an inflammatory CNS environment  (3).  Previously,  we  showed  that  CNS-infiltrated  immune  cells  (CNS-IICs)  in  the  experimental autoimmune  encephalomyelitis  (EAE)  rat,  rapidly  alter  the  activity  pattern  of  astrocytes  by  activating  glial  P2X7receptor  (P2X7R)(4).  In  the  present  study,  we  aimed  to  identify  the  properties of  astroglial  P2X7R  in  EAE  and  to identify mechanisms responsible for astrocyte activation in the presence of CNS-IICs (CD4+T cells). In this respect, spinal cords from rats at the peak of EAE and age-matched healthy controls were isolated and protein expression of P2X7R and connexin-43 (Cx-43) were investigated. P2X7R protein expression was decreased in the lumbar spinal cord, while Cx-43 did not change. Next, we found that P2X7R and Cx-43 proteins interact in the lumbar spinal cord since both the monomer and the dimer Cx-43 co-immunoprecipitate with P2X7R. Even though the colocalization of P2X7R and Cx-43 was decreased in EAE compared to the control, the analysis of the distribution of astroglial P2X7R and Cx-43 and their colocalization in the radius of 20 μm from the infiltrated CD4+T cell center showed that astroglial P2X7R and Cx-43 are specifically associated and concentrated in the proximity of CNS-IICs in the EAE spinal cord. Subsequently,  to  achieve  an  unambiguous  analysis  of  astrocyte-immune  cell  interaction,  we  monitored  Ca2+dynamics in Fluo-4 labeled cultured naïve astrocytes following brief bath-application of CNS-IICs isolated and purified from spinal cords of EAE rats. Our data suggest that astroglial αvβ3-integrin acted upstream of P2X7R activation and is likely involved in establishing initial contact of astrocytes with CNS-IICs since astrocytic αvβ3-integrin block reduced the astrocytic Ca2+response to CNS-IIC application. Furthermore, astrocytes challenged with CGP31157 (blocker of mNCLX and HCX) exhibited a prolonged intracellular Ca2+elevation and higher ATP release after brief exposure to CNS-IICs, indicating a regulatory function of mitochondria on this intracellular astrocyte Ca2+response. Collectively these  data  describing  integrin-relevant  cellular  mechanisms  of  astroglial  P2X7R  activation  could  help  to  expand integrin-inhibiting therapeutic approaches currently in use for MS treatment toward control ofastrocyte purine-based interaction with immune cells.
PB  - Wiley Periodicals LLC
C3  - XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet
T1  - P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells
SP  - E941
VL  - 71
DO  - https://doi.org/10.1002/glia.24419
ER  - 

@conference{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "Astrocytes form a dense meshwork throughout the central nervous system (CNS) which qualifies them to perform interactive maintenance functions with neighboring cells. In neuroinflammation, this astroglial cell-to-cell interaction varies which can either promote or lessen pathological processes (1,2). In multiple sclerosis (MS), astrocytes engage in an interaction with immune cells which drives neurodegeneration by creating and sustaining an inflammatory CNS environment  (3).  Previously,  we  showed  that  CNS-infiltrated  immune  cells  (CNS-IICs)  in  the  experimental autoimmune  encephalomyelitis  (EAE)  rat,  rapidly  alter  the  activity  pattern  of  astrocytes  by  activating  glial  P2X7receptor  (P2X7R)(4).  In  the  present  study,  we  aimed  to  identify  the  properties of  astroglial  P2X7R  in  EAE  and  to identify mechanisms responsible for astrocyte activation in the presence of CNS-IICs (CD4+T cells). In this respect, spinal cords from rats at the peak of EAE and age-matched healthy controls were isolated and protein expression of P2X7R and connexin-43 (Cx-43) were investigated. P2X7R protein expression was decreased in the lumbar spinal cord, while Cx-43 did not change. Next, we found that P2X7R and Cx-43 proteins interact in the lumbar spinal cord since both the monomer and the dimer Cx-43 co-immunoprecipitate with P2X7R. Even though the colocalization of P2X7R and Cx-43 was decreased in EAE compared to the control, the analysis of the distribution of astroglial P2X7R and Cx-43 and their colocalization in the radius of 20 μm from the infiltrated CD4+T cell center showed that astroglial P2X7R and Cx-43 are specifically associated and concentrated in the proximity of CNS-IICs in the EAE spinal cord. Subsequently,  to  achieve  an  unambiguous  analysis  of  astrocyte-immune  cell  interaction,  we  monitored  Ca2+dynamics in Fluo-4 labeled cultured naïve astrocytes following brief bath-application of CNS-IICs isolated and purified from spinal cords of EAE rats. Our data suggest that astroglial αvβ3-integrin acted upstream of P2X7R activation and is likely involved in establishing initial contact of astrocytes with CNS-IICs since astrocytic αvβ3-integrin block reduced the astrocytic Ca2+response to CNS-IIC application. Furthermore, astrocytes challenged with CGP31157 (blocker of mNCLX and HCX) exhibited a prolonged intracellular Ca2+elevation and higher ATP release after brief exposure to CNS-IICs, indicating a regulatory function of mitochondria on this intracellular astrocyte Ca2+response. Collectively these  data  describing  integrin-relevant  cellular  mechanisms  of  astroglial  P2X7R  activation  could  help  to  expand integrin-inhibiting therapeutic approaches currently in use for MS treatment toward control ofastrocyte purine-based interaction with immune cells.",
publisher = "Wiley Periodicals LLC",
journal = "XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet",
title = "P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells",
pages = "E941",
volume = "71",
doi = "https://doi.org/10.1002/glia.24419"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells. in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet
Wiley Periodicals LLC., 71, E941.
https://doi.org/https://doi.org/10.1002/glia.24419

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells. in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet. 2023;71:E941.
doi:https://doi.org/10.1002/glia.24419 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "P2X7R, β3-integrin and Cx-43 mediate interaction between astrocytes and adjacent autoreactive immune cells" in XVI European Meeting on Glial Cells in Health and Disease, Abstract booklet, 71 (2023):E941,
https://doi.org/https://doi.org/10.1002/glia.24419 . .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2014
AB  - The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.
PB  - Serbian Neuroscience Society
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells
SP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2014
ER  - 

@conference{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "The astrocytic network maintains homeostasis in the central nervous system (CNS) 
through interactions with neighboring cells. In the CNS autoimmune disease, multiple 
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. 
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) 
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, 
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor 
(P2X7R). In the present study we further defined the mechanisms responsible for 
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in 
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured 
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal 
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in 
the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced 
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial 
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the 
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, 
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. 
Overall, our study expands the understanding of astrocytes’ interaction with 
autoreactive immune cells that are present in their local environment in an 
autoimmune disease. This offers a new conceptual framework for considering direct 
astrocyte–immune cell interaction to design new strategies for therapy development in 
the treatment of MS.",
publisher = "Serbian Neuroscience Society",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells",
pages = "101",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2014"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Serbian Neuroscience Society., 101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:101.
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "αVβ3-Integrin and mitochondria mediate astrocyte response to  autoreactive immune cells" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):101,
https://hdl.handle.net/21.15107/rcub_rimsi_2014 .

TY  - JOUR
AU  - Milicevic, Katarina
AU  - Bataveljic, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Andjus, Pavle
AU  - Nikolic, Ljiljana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2007
AB  - In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.
PB  - MDPI
T2  - Cells
T1  - Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43
IS  - 13
SP  - 1786
VL  - 12
DO  - https://doi.org/10.3390/cells12131786
ER  - 

@article{
author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana",
year = "2023",
abstract = "In multiple sclerosis (MS), glial cells astrocytes interact with the autoreactive immune cells that attack the central nervous system (CNS), which causes and sustains neuroinflammation. However, little is known about the direct interaction between these cells when they are in close proximity in the inflamed CNS. By using an experimental autoimmune encephalomyelitis (EAE) model of MS, we previously found that in the proximity of autoreactive CNS-infiltrated immune cells (CNS-IICs), astrocytes respond with a rapid calcium increase that is mediated by the autocrine P2X7 receptor (P2X7R) activation. We now reveal that the mechanisms regulating this direct interaction of astrocytes and CNS-IICs involve the coupling between P2X7R, connexin-43, and β3-integrin. We found that P2X7R and astroglial connexin-43 interact and concentrate in the immediate proximity of the CNS-IICs in EAE. P2X7R also interacts with β3-integrin, and the block of astroglial αvβ3-integrin reduces the P2X7R-dependent calcium response of astrocytes upon encountering CNS-IICs. This interaction was dependent on astroglial mitochondrial activity, which regulated the ATP-driven P2X7R activation and facilitated the termination of the astrocytic calcium response evoked by CNS-IICs. By further defining the interactions between the CNS and the immune system, our findings provide a novel perspective toward expanding integrin-targeting therapeutic approaches for MS treatment by controlling the cell–cell interactions between astrocytes and CNS-IICs.",
publisher = "MDPI",
journal = "Cells",
title = "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43",
number = "13",
pages = "1786",
volume = "12",
doi = "https://doi.org/10.3390/cells12131786"
}

Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells
MDPI., 12(13), 1786.
https://doi.org/https://doi.org/10.3390/cells12131786

Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43. in Cells. 2023;12(13):1786.
doi:https://doi.org/10.3390/cells12131786 .

Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "Astroglial Cell-to-Cell Interaction with Autoreactive Immune  Cells in Experimental Autoimmune Encephalomyelitis Involves  P2X7 Receptor, 3-Integrin, and Connexin-43" in Cells, 12, no. 13 (2023):1786,
https://doi.org/https://doi.org/10.3390/cells12131786 . .

TY  - JOUR
AU  - Jovičić, Katarina
AU  - Janković, Saša
AU  - Nikolić, Dragica
AU  - Đikanović, Vesna
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
AU  - Jarić, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1731
AB  - The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As. The results suggest that scales and fins have a potential to be used as indicators of muscle tissue contamination with As, Cu, and Hg, depending on species’ ecological traits.
PB  - EDP Sciences
T2  - Knowledge & Management of Aquatic Ecosystems
T1  - Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River
IS  - 4
VL  - 424
DO  - https://doi.org/10.1051/kmae/2022027
ER  - 

@article{
author = "Jovičić, Katarina and Janković, Saša and Nikolić, Dragica and Đikanović, Vesna and Skorić, Stefan and Krpo-Ćetković, Jasmina and Jarić, Ivan",
year = "2023",
abstract = "The development of nonlethal methods for the monitoring of environmental contamination is
essential to minimize the negative effects on studied species and communities. Fish scales and fin clips can be used as nonlethal indicators of water quality given that they are in direct contact with the environment and can accumulate high concentrations of metals and trace elements. Fin clipping causes minimal harm to fish and it does not affect fish growth or survival. In this study, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn concentrations were measured by inductively-coupled plasma mass spectrometry (ICP-MS) in scales, fins, and muscle of common bream (Abramis brama), white bream (Blicca bjoerkna), wels catfish (Silurus glanis), northern pike (Esox lucius), and pikeperch (Sander lucioperca) from the Danube River. The analysis showed a positive correlation for Hg between scales and muscle in pikeperch. Anal fin and muscle were positively correlated in white bream for Hg, in wels catfish for Cu, and in northern pike and pikeperch for As. The results suggest that scales and fins have a potential to be used as indicators of muscle tissue contamination with As, Cu, and Hg, depending on species’ ecological traits.",
publisher = "EDP Sciences",
journal = "Knowledge & Management of Aquatic Ecosystems",
title = "Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River",
number = "4",
volume = "424",
doi = "https://doi.org/10.1051/kmae/2022027"
}

Jovičić, K., Janković, S., Nikolić, D., Đikanović, V., Skorić, S., Krpo-Ćetković, J.,& Jarić, I.. (2023). Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River. in Knowledge & Management of Aquatic Ecosystems
EDP Sciences., 424(4).
https://doi.org/https://doi.org/10.1051/kmae/2022027

Jovičić K, Janković S, Nikolić D, Đikanović V, Skorić S, Krpo-Ćetković J, Jarić I. Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River. in Knowledge & Management of Aquatic Ecosystems. 2023;424(4).
doi:https://doi.org/10.1051/kmae/2022027 .

Jovičić, Katarina, Janković, Saša, Nikolić, Dragica, Đikanović, Vesna, Skorić, Stefan, Krpo-Ćetković, Jasmina, Jarić, Ivan, "Prospects of fish scale and fin samples usage for nonlethal monitoring of metal contamination: a study on five fish species from the Danube River" in Knowledge & Management of Aquatic Ecosystems, 424, no. 4 (2023),
https://doi.org/https://doi.org/10.1051/kmae/2022027 . .

TY  - CONF
AU  - Rodić, Ivanka
AU  - Lukičić, Jovana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2409
AB  - Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite
EP  - 233
SP  - 233
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2409
ER  - 

@conference{
author = "Rodić, Ivanka and Lukičić, Jovana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana",
year = "2022",
abstract = "Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite",
pages = "233-233",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2409"
}

Rodić, I., Lukičić, J., Stanić, M., Žižić, M., Zakrzewska, J., Živić, M.,& Cvetic Antic, T.. (2022). Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409

Rodić I, Lukičić J, Stanić M, Žižić M, Zakrzewska J, Živić M, Cvetic Antic T. Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

Rodić, Ivanka, Lukičić, Jovana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):233-233,
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Rodić, Ivanka
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Todorović, Nataša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2114
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 92
SP  - 89
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2114
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and Rodić, Ivanka and Cvetic Antic, Tijana and Živić, Miroslav and Zakrzewska, Joanna and Todorović, Nataša",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "92-89",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2114"
}

Žižić, M., Stanić, M., Rodić, I., Cvetic Antic, T., Živić, M., Zakrzewska, J.,& Todorović, N.. (2022). PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114

Žižić M, Stanić M, Rodić I, Cvetic Antic T, Živić M, Zakrzewska J, Todorović N. PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

Žižić, Milan, Stanić, Marina, Rodić, Ivanka, Cvetic Antic, Tijana, Živić, Miroslav, Zakrzewska, Joanna, Todorović, Nataša, "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):89-92,
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

TY  - CONF
AU  - Janošević, Dušica
AU  - Vojisavljević, Katarina
AU  - Pecinar, Ilinka
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1812
AB  - The plant cell wall (CW) is a cell compartment lying outside the plasma
membrane forming a continuum throughout the plant body. The composition of
CWs varies between plant species and cell types, while changes in CW constituents
occur as a result of growth and developmental processes, or as a response to various
environmental stimuli. In the herein study, we present a selection of microscopic
methods for rapid, relatively simple, and low-cost visualization of CW
structure/composition in tissues of stems in trees, shrubs, or herbs. Selected
microscopic methods imply the use of unfixed, intact, both fresh and dried, plant
tissues for the analysis, as well as free-hand or microtome sectioning. UV
microscopy, phloroglucinol-HCl and toluidine blue O staining, SEM microscopy,
and RAMAN microspectroscopy, separately or in combination, can provide valuable
information in plant ecology, plant physiology studies, or for applications in
agronomy and forest products industry. As a model, stem samples of juvenile
Populus x euramericana trees exposed to severe long term static bending were
selected: tension wood fibers (gelatinous fibers, specialized sclerenchyma cells)
could be considered as representatives of CWs with the most complex structure,
while the genus Populus is considered as a model woody Angiosperm.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia
C3  - 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022
T1  - Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties
SP  - 201
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1812
ER  - 

@conference{
author = "Janošević, Dušica and Vojisavljević, Katarina and Pecinar, Ilinka and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "The plant cell wall (CW) is a cell compartment lying outside the plasma
membrane forming a continuum throughout the plant body. The composition of
CWs varies between plant species and cell types, while changes in CW constituents
occur as a result of growth and developmental processes, or as a response to various
environmental stimuli. In the herein study, we present a selection of microscopic
methods for rapid, relatively simple, and low-cost visualization of CW
structure/composition in tissues of stems in trees, shrubs, or herbs. Selected
microscopic methods imply the use of unfixed, intact, both fresh and dried, plant
tissues for the analysis, as well as free-hand or microtome sectioning. UV
microscopy, phloroglucinol-HCl and toluidine blue O staining, SEM microscopy,
and RAMAN microspectroscopy, separately or in combination, can provide valuable
information in plant ecology, plant physiology studies, or for applications in
agronomy and forest products industry. As a model, stem samples of juvenile
Populus x euramericana trees exposed to severe long term static bending were
selected: tension wood fibers (gelatinous fibers, specialized sclerenchyma cells)
could be considered as representatives of CWs with the most complex structure,
while the genus Populus is considered as a model woody Angiosperm.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia",
journal = "14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022",
title = "Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties",
pages = "201",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1812"
}

Janošević, D., Vojisavljević, K., Pecinar, I.,& Mitrović, A. Lj.. (2022). Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties. in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš Institute for Nature Conservation of Serbia., 201.
https://hdl.handle.net/21.15107/rcub_rimsi_1812

Janošević D, Vojisavljević K, Pecinar I, Mitrović AL. Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties. in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022. 2022;:201.
https://hdl.handle.net/21.15107/rcub_rimsi_1812 .

Janošević, Dušica, Vojisavljević, Katarina, Pecinar, Ilinka, Mitrović, Aleksandra Lj., "Populus x euramericana tension wood as a model for selection of microscopic methods for rapid screening of cell wall structure in the analysis of plant stem properties" in 14 th Symposium on the Flora of Southeastern Serbia and Neighboring Regions Kladovo, 26 th to 29 thJune, 2022 (2022):201,
https://hdl.handle.net/21.15107/rcub_rimsi_1812 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Hladnik, Matjaž
AU  - Janakiev, Tamara
AU  - Bandelj, Dunja
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2143
AB  - INTRODUCTION Maize is among the three world’s most important and widely grown cereals (Seyi-Amole & Onilude, 2021). The excessive and long-term application of agrochemicals for providing maize with essential nutrients, required for the development of all growth phenophases and for yield enhancement, leaves adverse consequences for human health and the environment (Khaliq et al., 2004). Although their use is inevitable to meet the increasing demand of the growing human population for a healthy food supply, organic fertilizers and biofertilizers (microbial fertilizers) are becoming recognized as effective, economically feasible, and environmentally sound alternatives for sustainable agriculture (Lawal & Babalola, 2014; Hui et al., 2017; Mahanty et al., 2017). The main objective of this study was to evaluate the influence of the microbial inoculant Phytobiotic (PHY), containing a consortium of Bacillus subtilis sp. subtilis and Microbacterium sp., on native maize microbiome during key growth phenophases (seedling, flowering, and harvesting) under field conditions, as well as to compare whether differences in efficacy between PHY, poultry manure (PM) and their combination (PHY_PM) exist, based on yield parameters. 
STATE OF THE ART Seeds, roots, and soil samples were taken for metabarcoding analysis during four growth phenophases (I-IV). Samples of uninoculated seeds and soil, poultry manure, and seeds inoculated with PHY were primarily taken before sowing (phenophase I). Further, during the growing season [phenophases II (seedling), III (flowering), and IV (harvesting)] the effect of PHY, PM, and PHY_PM on maize seeds, roots, and soil microbiome was evaluated in relation to concurrently sampled negative controls. A total DNA from the collected samples was isolated, amplified with primers 515F/ 806R targeting the V4 region of the 16S rRNA, and subjected to next-generation sequencing (NGS). The obtained sequence data were bioinformatically processed and used for the evaluation of alpha and beta diversity. Yield and associated parameters (number of grown and fallen/broken plants, rating fence, plant vigor, the occurrence of Ustilago sp., and grain moisture) were evaluated after harvest. 
RESULTS Seeds exibited lower bacterial diversity compared to the soil, root, and manure samples.The most abundant taxon in uninoculated seeds pre-harvest was Pantoea, while in seeds treated with PHY the most abundant was Acinetobacter, followed by Pantoea, Pseudomonas, and Bacillus. After harvest, Pantoea and Pseudomonas prevearled in seeds. Soil bacterial communities mostly remained unchanged, regardless of the treatment (PHY, PM, and PHY_PM) applied or the tested phenophase, with uncultured Gaiellales and Bacillus being the most abundant. Contrarily, root bacterial communities differed in distribution and relative abundance of different taxa between phenophases and between treatments. The most abundant taxa in roots during the inial phenophase (II) was Pseudomonas. In the flowering phenophase (III), Bacillus prevealed with two to three times higher relative abundance in treatments with PHY or PM compared to the negative control, while Lechevalieria dominated in harvesting phenophase (IV). A statistically significant increase in maize yield was obtained in the treatment with PHY, with an average value of 650 kg/ha compared to the negative control. The lowest yield was obtained in the treatment with PM. DISCUSSION The prevalence of Acinetobacter, Pantoea, Pseudomonas, and Bacillus in seeds treated with PHY pre-harvest, indicates that treatment with PHY is highly beneficial considering the known plant growth promoting potential of these genera, that were also previously confirmed as core maize inhabitants (Mehta et al., 2021). As core members, Pantoea and Pseudomonas remained present after harvest. The benefit of the application of Bacillus-based fertilizers to soil is the enhancement of the plant-available forms of nutrients and the inducement of pest and pathogens defense systems (Radhakrishnan, et al., 2017). It is of crucial importance that none of the three treatments applied in this study affected the composition of the indigenous soil bacterial communities during four tested phenophases, which is highly important when selecting suitable agricultural practices. Shifts in root microbiome over maize growth could be related to the production of different root metabolites over the growing season (Bourceret et al., 2022). Roots were especially rich with genera (Pseudomonas, Stenotrophomonas, Sphingobacterium, Achromobacter) known as phosphate solubilizers (Mehta et al., 2021). Furthermore, Bacillus was dominant in roots in flowering phenophase. This genus is known for its wide spectrum of beneficial effects on plants, like phosphate solubilization, biosynthesis of growth hormones, antimicrobial activity, induction of systemic resistance, etc. (Dimkic et al., 2022). CONCLUSIONS Considering the above-mentioned effect of PHY on maize yield incensement, its non-disruption effect on the core microbiome, and the positive effect on enhancing the presence of beneficial bacterial genera, this microbial inoculant could be proposed as a promising alternative to chemicals and organic fertilizers in maize cultivation.
PB  - Jožef Stefan Institute Press.
C3  - WEEK OF MICROBIAL TECHNOLOGIES, Ljubljana, Slovenia, November 7 – 11, 2022
T1  - INFLUENCE OF MICROBIAL AND ORGANIC FERTILIZERS ON BACTERIAL COMMUNITIES COMPOSITION DURING KEY GROWTH PHENOPHASES OF MAIZE
EP  - 52
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2143
ER  - 

@conference{
author = "Jelušić, Aleksandra and Hladnik, Matjaž and Janakiev, Tamara and Bandelj, Dunja and Dimkić, Ivica",
year = "2022",
abstract = "INTRODUCTION Maize is among the three world’s most important and widely grown cereals (Seyi-Amole & Onilude, 2021). The excessive and long-term application of agrochemicals for providing maize with essential nutrients, required for the development of all growth phenophases and for yield enhancement, leaves adverse consequences for human health and the environment (Khaliq et al., 2004). Although their use is inevitable to meet the increasing demand of the growing human population for a healthy food supply, organic fertilizers and biofertilizers (microbial fertilizers) are becoming recognized as effective, economically feasible, and environmentally sound alternatives for sustainable agriculture (Lawal & Babalola, 2014; Hui et al., 2017; Mahanty et al., 2017). The main objective of this study was to evaluate the influence of the microbial inoculant Phytobiotic (PHY), containing a consortium of Bacillus subtilis sp. subtilis and Microbacterium sp., on native maize microbiome during key growth phenophases (seedling, flowering, and harvesting) under field conditions, as well as to compare whether differences in efficacy between PHY, poultry manure (PM) and their combination (PHY_PM) exist, based on yield parameters. 
STATE OF THE ART Seeds, roots, and soil samples were taken for metabarcoding analysis during four growth phenophases (I-IV). Samples of uninoculated seeds and soil, poultry manure, and seeds inoculated with PHY were primarily taken before sowing (phenophase I). Further, during the growing season [phenophases II (seedling), III (flowering), and IV (harvesting)] the effect of PHY, PM, and PHY_PM on maize seeds, roots, and soil microbiome was evaluated in relation to concurrently sampled negative controls. A total DNA from the collected samples was isolated, amplified with primers 515F/ 806R targeting the V4 region of the 16S rRNA, and subjected to next-generation sequencing (NGS). The obtained sequence data were bioinformatically processed and used for the evaluation of alpha and beta diversity. Yield and associated parameters (number of grown and fallen/broken plants, rating fence, plant vigor, the occurrence of Ustilago sp., and grain moisture) were evaluated after harvest. 
RESULTS Seeds exibited lower bacterial diversity compared to the soil, root, and manure samples.The most abundant taxon in uninoculated seeds pre-harvest was Pantoea, while in seeds treated with PHY the most abundant was Acinetobacter, followed by Pantoea, Pseudomonas, and Bacillus. After harvest, Pantoea and Pseudomonas prevearled in seeds. Soil bacterial communities mostly remained unchanged, regardless of the treatment (PHY, PM, and PHY_PM) applied or the tested phenophase, with uncultured Gaiellales and Bacillus being the most abundant. Contrarily, root bacterial communities differed in distribution and relative abundance of different taxa between phenophases and between treatments. The most abundant taxa in roots during the inial phenophase (II) was Pseudomonas. In the flowering phenophase (III), Bacillus prevealed with two to three times higher relative abundance in treatments with PHY or PM compared to the negative control, while Lechevalieria dominated in harvesting phenophase (IV). A statistically significant increase in maize yield was obtained in the treatment with PHY, with an average value of 650 kg/ha compared to the negative control. The lowest yield was obtained in the treatment with PM. DISCUSSION The prevalence of Acinetobacter, Pantoea, Pseudomonas, and Bacillus in seeds treated with PHY pre-harvest, indicates that treatment with PHY is highly beneficial considering the known plant growth promoting potential of these genera, that were also previously confirmed as core maize inhabitants (Mehta et al., 2021). As core members, Pantoea and Pseudomonas remained present after harvest. The benefit of the application of Bacillus-based fertilizers to soil is the enhancement of the plant-available forms of nutrients and the inducement of pest and pathogens defense systems (Radhakrishnan, et al., 2017). It is of crucial importance that none of the three treatments applied in this study affected the composition of the indigenous soil bacterial communities during four tested phenophases, which is highly important when selecting suitable agricultural practices. Shifts in root microbiome over maize growth could be related to the production of different root metabolites over the growing season (Bourceret et al., 2022). Roots were especially rich with genera (Pseudomonas, Stenotrophomonas, Sphingobacterium, Achromobacter) known as phosphate solubilizers (Mehta et al., 2021). Furthermore, Bacillus was dominant in roots in flowering phenophase. This genus is known for its wide spectrum of beneficial effects on plants, like phosphate solubilization, biosynthesis of growth hormones, antimicrobial activity, induction of systemic resistance, etc. (Dimkic et al., 2022). CONCLUSIONS Considering the above-mentioned effect of PHY on maize yield incensement, its non-disruption effect on the core microbiome, and the positive effect on enhancing the presence of beneficial bacterial genera, this microbial inoculant could be proposed as a promising alternative to chemicals and organic fertilizers in maize cultivation.",
publisher = "Jožef Stefan Institute Press.",
journal = "WEEK OF MICROBIAL TECHNOLOGIES, Ljubljana, Slovenia, November 7 – 11, 2022",
title = "INFLUENCE OF MICROBIAL AND ORGANIC FERTILIZERS ON BACTERIAL COMMUNITIES COMPOSITION DURING KEY GROWTH PHENOPHASES OF MAIZE",
pages = "52-50",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2143"
}

Jelušić, A., Hladnik, M., Janakiev, T., Bandelj, D.,& Dimkić, I.. (2022). INFLUENCE OF MICROBIAL AND ORGANIC FERTILIZERS ON BACTERIAL COMMUNITIES COMPOSITION DURING KEY GROWTH PHENOPHASES OF MAIZE. in WEEK OF MICROBIAL TECHNOLOGIES, Ljubljana, Slovenia, November 7 – 11, 2022
Jožef Stefan Institute Press.., 50-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2143

Jelušić A, Hladnik M, Janakiev T, Bandelj D, Dimkić I. INFLUENCE OF MICROBIAL AND ORGANIC FERTILIZERS ON BACTERIAL COMMUNITIES COMPOSITION DURING KEY GROWTH PHENOPHASES OF MAIZE. in WEEK OF MICROBIAL TECHNOLOGIES, Ljubljana, Slovenia, November 7 – 11, 2022. 2022;:50-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2143 .

Jelušić, Aleksandra, Hladnik, Matjaž, Janakiev, Tamara, Bandelj, Dunja, Dimkić, Ivica, "INFLUENCE OF MICROBIAL AND ORGANIC FERTILIZERS ON BACTERIAL COMMUNITIES COMPOSITION DURING KEY GROWTH PHENOPHASES OF MAIZE" in WEEK OF MICROBIAL TECHNOLOGIES, Ljubljana, Slovenia, November 7 – 11, 2022 (2022):50-52,
https://hdl.handle.net/21.15107/rcub_rimsi_2143 .

TY  - CONF
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Trkulja, Nenad
AU  - Živković, Ivana
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2138
AB  - The biocontrol ability of Bacillus halotolerans strain B33 against the most significant stone fruit bacterial and fungal pathogens was investigated under in vitro conditions. The results indicate that the inhibition zone toward bacterial pathogen Xanthomonas arboricola pv. pruni originating from peach and apricot (strains Xp219 and Xp320, respectively) ranged from 20 to 45 mm in diameter,
depending of the used B. halotolerans concentration. B. halotolerans B33 did not inhibit the growth of bacteria Pseudomonas syringae pv. syringae (strains RE05 and RE3 originating from sweet cherry), P. s. pv. morsprunorum race 1 (strain Pm5 originating from sweet cherry and Pm26 from plum) and P. cerasi (strain RE10 originating from wild cherry). In the case of fungal pathogen 
Monilinia fructicola strain 116, 41.66−61.00% growth inhibition was observed when pure culture of B. halotolerans was used. From the obtained results in this work, B. halotolerans strain B33 could be recommended to be potentially used as a suitable biocontrol agent for the control of Xanthomonads plant pathogenic bacteria and Monilinia spp. on stone fruit.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade
C3  - 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
T1  - BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS
EP  - 179
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2138
ER  - 

@conference{
author = "Iličić, Renata and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Bagi, Ferenc and Trkulja, Nenad and Živković, Ivana and Stanković, Slaviša",
year = "2022",
abstract = "The biocontrol ability of Bacillus halotolerans strain B33 against the most significant stone fruit bacterial and fungal pathogens was investigated under in vitro conditions. The results indicate that the inhibition zone toward bacterial pathogen Xanthomonas arboricola pv. pruni originating from peach and apricot (strains Xp219 and Xp320, respectively) ranged from 20 to 45 mm in diameter,
depending of the used B. halotolerans concentration. B. halotolerans B33 did not inhibit the growth of bacteria Pseudomonas syringae pv. syringae (strains RE05 and RE3 originating from sweet cherry), P. s. pv. morsprunorum race 1 (strain Pm5 originating from sweet cherry and Pm26 from plum) and P. cerasi (strain RE10 originating from wild cherry). In the case of fungal pathogen 
Monilinia fructicola strain 116, 41.66−61.00% growth inhibition was observed when pure culture of B. halotolerans was used. From the obtained results in this work, B. halotolerans strain B33 could be recommended to be potentially used as a suitable biocontrol agent for the control of Xanthomonads plant pathogenic bacteria and Monilinia spp. on stone fruit.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade",
journal = "4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection",
title = "BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS",
pages = "179-170",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2138"
}

Iličić, R., Popović Milovanović, T., Jelušić, A., Bagi, F., Trkulja, N., Živković, I.,& Stanković, S.. (2022). BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS. in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences Belgrade., 170-179.
https://hdl.handle.net/21.15107/rcub_rimsi_2138

Iličić R, Popović Milovanović T, Jelušić A, Bagi F, Trkulja N, Živković I, Stanković S. BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS. in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection. 2022;:170-179.
https://hdl.handle.net/21.15107/rcub_rimsi_2138 .

Iličić, Renata, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Bagi, Ferenc, Trkulja, Nenad, Živković, Ivana, Stanković, Slaviša, "BIOCONTROL ABILITY OF BACILLUS HALOTOLERANS AGAINST STONE FRUIT PATHOGENS" in 4th International Symposium: Modern Trends in Agricultural Production, Rural Development, Agro-economy, Cooperatives and Environmental Protection (2022):170-179,
https://hdl.handle.net/21.15107/rcub_rimsi_2138 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Stanisavljević, Rade
AU  - Janakiev, Tamara
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2047
AB  - The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid
formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i)
seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were
used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found
between disease intensity and cabbage heads’ weight.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy
T1  - Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’
EP  - 147
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2047
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Mitrović, Petar and Stanisavljević, Rade and Janakiev, Tamara and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid
formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i)
seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were
used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found
between disease intensity and cabbage heads’ weight.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy",
title = "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2047"
}

Jelušić, A., Popović Milovanović, T., Mitrović, P., Stanisavljević, R., Janakiev, T., Fira, Đ.,& Dimkić, I.. (2022). Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini., 147-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2047

Jelušić A, Popović Milovanović T, Mitrović P, Stanisavljević R, Janakiev T, Fira Đ, Dimkić I. Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy. 2022;:147-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2047 .

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Mitrović, Petar, Stanisavljević, Rade, Janakiev, Tamara, Fira, Đorđe, Dimkić, Ivica, "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’" in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy (2022):147-147,
https://hdl.handle.net/21.15107/rcub_rimsi_2047 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1914
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.
PB  - Springer
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres
EP  - 18
SP  - 1
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.",
publisher = "Springer",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres",
pages = "18-1",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research
Springer., 1-18.
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research. 2022;:1-18.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres" in Potato Research (2022):1-18,
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - JOUR
AU  - Nobili, Paola
AU  - SHEN, Weida
AU  - Milicevic, Katarina
AU  - Bogdanović Pristov, Jelena
AU  - Audinat, Etienne
AU  - Nikolic, Ljiljana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1813
AB  - Epilepsy and multiple sclerosis (MS), two of the most common neurological diseases, are
characterized by the establishment of inflammatory environment in the central nervous
system that drives disease progression and impacts on neurodegeneration. Current
therapeutic approaches in the treatments of epilepsy and MS are targeting neuronal
activity and immune cell response, respectively. However, the lack of fully efficient
responses to the available treatments obviously shows the need to search for novel
therapeutic candidates that will not exclusively target neurons or immune cells.
Accumulating knowledge on epilepsy and MS in humans and analysis of relevant
animal models, reveals that astrocytes are promising therapeutic candidates to target
as they participate in the modulation of the neuroinflammatory response in both diseases
from the initial stages and may play an important role in their development. Indeed,
astrocytes respond to reactive immune cells and contribute to the neuronal hyperactivity in
the inflamed brain. Mechanistically, these astrocytic cell to cell interactions are
fundamentally mediated by the purinergic signalling and involve metabotropic P2Y1
receptors in case of astrocyte interactions with neurons, while ionotropic P2X7
receptors are mainly involved in astrocyte interactions with autoreactive immune cells.
Herein, we review the potential of targeting astrocytic purinergic signalling mediated by
P2Y1 and P2X7 receptors to develop novel approaches for treatments of epilepsy and MS
at very early stages.
PB  - Frontiers Media
T2  - Frontiers in Pharmacology
T1  - Therapeutic Potential of Astrocyte Purinergic Signalling in Epilepsy and Multiple Sclerosis
SP  - 900337
VL  - 13
DO  - 10.3389/fphar.2022.900337
ER  - 

@article{
author = "Nobili, Paola and SHEN, Weida and Milicevic, Katarina and Bogdanović Pristov, Jelena and Audinat, Etienne and Nikolic, Ljiljana",
year = "2022",
abstract = "Epilepsy and multiple sclerosis (MS), two of the most common neurological diseases, are
characterized by the establishment of inflammatory environment in the central nervous
system that drives disease progression and impacts on neurodegeneration. Current
therapeutic approaches in the treatments of epilepsy and MS are targeting neuronal
activity and immune cell response, respectively. However, the lack of fully efficient
responses to the available treatments obviously shows the need to search for novel
therapeutic candidates that will not exclusively target neurons or immune cells.
Accumulating knowledge on epilepsy and MS in humans and analysis of relevant
animal models, reveals that astrocytes are promising therapeutic candidates to target
as they participate in the modulation of the neuroinflammatory response in both diseases
from the initial stages and may play an important role in their development. Indeed,
astrocytes respond to reactive immune cells and contribute to the neuronal hyperactivity in
the inflamed brain. Mechanistically, these astrocytic cell to cell interactions are
fundamentally mediated by the purinergic signalling and involve metabotropic P2Y1
receptors in case of astrocyte interactions with neurons, while ionotropic P2X7
receptors are mainly involved in astrocyte interactions with autoreactive immune cells.
Herein, we review the potential of targeting astrocytic purinergic signalling mediated by
P2Y1 and P2X7 receptors to develop novel approaches for treatments of epilepsy and MS
at very early stages.",
publisher = "Frontiers Media",
journal = "Frontiers in Pharmacology",
title = "Therapeutic Potential of Astrocyte Purinergic Signalling in Epilepsy and Multiple Sclerosis",
pages = "900337",
volume = "13",
doi = "10.3389/fphar.2022.900337"
}

Nobili, P., SHEN, W., Milicevic, K., Bogdanović Pristov, J., Audinat, E.,& Nikolic, L.. (2022). Therapeutic Potential of Astrocyte Purinergic Signalling in Epilepsy and Multiple Sclerosis. in Frontiers in Pharmacology
Frontiers Media., 13, 900337.
https://doi.org/10.3389/fphar.2022.900337

Nobili P, SHEN W, Milicevic K, Bogdanović Pristov J, Audinat E, Nikolic L. Therapeutic Potential of Astrocyte Purinergic Signalling in Epilepsy and Multiple Sclerosis. in Frontiers in Pharmacology. 2022;13:900337.
doi:10.3389/fphar.2022.900337 .

Nobili, Paola, SHEN, Weida, Milicevic, Katarina, Bogdanović Pristov, Jelena, Audinat, Etienne, Nikolic, Ljiljana, "Therapeutic Potential of Astrocyte Purinergic Signalling in Epilepsy and Multiple Sclerosis" in Frontiers in Pharmacology, 13 (2022):900337,
https://doi.org/10.3389/fphar.2022.900337 . .