TY  - JOUR
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1914
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.
PB  - Springer
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres
EP  - 18
SP  - 1
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.",
publisher = "Springer",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres",
pages = "18-1",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research
Springer., 1-18.
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research. 2022;:1-18.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres" in Potato Research (2022):1-18,
https://doi.org/10.1007/s11540-022-09601-9 . .