TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Sosic-Jurjević, Branka T
AU  - Filipović, Branko R
AU  - Trifunović, Svetlana L
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/482
AB  - A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study
EP  - 135
IS  - 3
SP  - 131
VL  - 239
DO  - 10.1007/s00232-010-9332-8
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Sosic-Jurjević, Branka T and Filipović, Branko R and Trifunović, Svetlana L and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2011",
abstract = "A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study",
pages = "135-131",
number = "3",
volume = "239",
doi = "10.1007/s00232-010-9332-8"
}

Ajdzanović, V. Z., Spasojević, I., Sosic-Jurjević, B. T., Filipović, B. R., Trifunović, S. L., Sekulic, M. I.,& Milošević, V. Lj.. (2011). The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology
Springer, New York., 239(3), 131-135.
https://doi.org/10.1007/s00232-010-9332-8

Ajdzanović VZ, Spasojević I, Sosic-Jurjević BT, Filipović BR, Trifunović SL, Sekulic MI, Milošević VL. The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology. 2011;239(3):131-135.
doi:10.1007/s00232-010-9332-8 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Sosic-Jurjević, Branka T, Filipović, Branko R, Trifunović, Svetlana L, Sekulic, Milka I, Milošević, Verica Lj., "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study" in Journal of Membrane Biology, 239, no. 3 (2011):131-135,
https://doi.org/10.1007/s00232-010-9332-8 . .

TY  - JOUR
AU  - Milivojević, Jasminka M
AU  - Maksimović, Vuk
AU  - Nikolic, Milos
AU  - Bogdanović Pristov, Jelena
AU  - Maletic, Radojka
AU  - Milatović, Dragan P
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/439
AB  - Content of individual sugars, organic acids, total phenolics (TPH), some important phenolic compounds (kaempferol, myricetin, quercetin, and ellagic acid) and total antioxidant capacity (TAC) in the fruits of strawberry, raspberry and blackberry were studied. A comparison was made between cultivars and wild relatives of each species (Fragaria vesca L., Rubus idaeus L. and Rubus fruticosus L.). The main sugars found were fructose and glucose both in the fruits of wild species and the studied cultivars. Citric acid was determined to be the major organic acid in most of tested berries with the exception of blackberry cultivars, where malic acid was dominant. The content of individual phenolic compounds varied among the wild species, as well as among the studied cultivars. Ellagic acid content was higher in F. vesca, R. idaeus and R. fruticosus (122.5 mu g/g FW, 12.71 mu g/g FW and 61.7 mu g/g FW, respectively) than that obtained in analyzed cultivars. Overall, TPH expressed higher values in the wild strawberry and blackberry species in comparison to the studied cultivars, and consequently, the highest levels of TAC were recorded in F. vesca (5.78 mg asc/g FW), followed by R. fruticosus (4.95 mg asc/g FW).
PB  - Wiley-Hindawi, London
T2  - Journal of Food Quality
T1  - Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries
EP  - 9
IS  - 1
SP  - 1
VL  - 34
DO  - 10.1111/j.1745-4557.2010.00360.x
ER  - 

@article{
author = "Milivojević, Jasminka M and Maksimović, Vuk and Nikolic, Milos and Bogdanović Pristov, Jelena and Maletic, Radojka and Milatović, Dragan P",
year = "2011",
abstract = "Content of individual sugars, organic acids, total phenolics (TPH), some important phenolic compounds (kaempferol, myricetin, quercetin, and ellagic acid) and total antioxidant capacity (TAC) in the fruits of strawberry, raspberry and blackberry were studied. A comparison was made between cultivars and wild relatives of each species (Fragaria vesca L., Rubus idaeus L. and Rubus fruticosus L.). The main sugars found were fructose and glucose both in the fruits of wild species and the studied cultivars. Citric acid was determined to be the major organic acid in most of tested berries with the exception of blackberry cultivars, where malic acid was dominant. The content of individual phenolic compounds varied among the wild species, as well as among the studied cultivars. Ellagic acid content was higher in F. vesca, R. idaeus and R. fruticosus (122.5 mu g/g FW, 12.71 mu g/g FW and 61.7 mu g/g FW, respectively) than that obtained in analyzed cultivars. Overall, TPH expressed higher values in the wild strawberry and blackberry species in comparison to the studied cultivars, and consequently, the highest levels of TAC were recorded in F. vesca (5.78 mg asc/g FW), followed by R. fruticosus (4.95 mg asc/g FW).",
publisher = "Wiley-Hindawi, London",
journal = "Journal of Food Quality",
title = "Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries",
pages = "9-1",
number = "1",
volume = "34",
doi = "10.1111/j.1745-4557.2010.00360.x"
}

Milivojević, J. M., Maksimović, V., Nikolic, M., Bogdanović Pristov, J., Maletic, R.,& Milatović, D. P.. (2011). Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries. in Journal of Food Quality
Wiley-Hindawi, London., 34(1), 1-9.
https://doi.org/10.1111/j.1745-4557.2010.00360.x

Milivojević JM, Maksimović V, Nikolic M, Bogdanović Pristov J, Maletic R, Milatović DP. Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries. in Journal of Food Quality. 2011;34(1):1-9.
doi:10.1111/j.1745-4557.2010.00360.x .

Milivojević, Jasminka M, Maksimović, Vuk, Nikolic, Milos, Bogdanović Pristov, Jelena, Maletic, Radojka, Milatović, Dragan P, "Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries" in Journal of Food Quality, 34, no. 1 (2011):1-9,
https://doi.org/10.1111/j.1745-4557.2010.00360.x . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica D
AU  - Spasic, Snežana D
AU  - Jones, David R
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/417
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Maney Publishing, Leeds
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
EP  - 35
IS  - 1
SP  - 29
VL  - 15
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica D and Spasic, Snežana D and Jones, David R and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Maney Publishing, Leeds",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
pages = "35-29",
number = "1",
volume = "15",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z. D., Spasic, S. D., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Maney Publishing, Leeds., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević ZD, Spasic SD, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica D, Spasic, Snežana D, Jones, David R, Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Filipović, Branko R
AU  - Sosic-Jurjević, Branka T
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/414
AB  - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.
PB  - Natl Research Council Canada-N R C Research Press, Ottawa
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
EP  - 500
IS  - 4
SP  - 497
VL  - 88
DO  - 10.1139/Y10-020
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Filipović, Branko R and Sosic-Jurjević, Branka T and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2010",
abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.",
publisher = "Natl Research Council Canada-N R C Research Press, Ottawa",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study",
pages = "500-497",
number = "4",
volume = "88",
doi = "10.1139/Y10-020"
}

Ajdzanović, V. Z., Spasojević, I., Filipović, B. R., Sosic-Jurjević, B. T., Sekulic, M. I.,& Milošević, V. Lj.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology
Natl Research Council Canada-N R C Research Press, Ottawa., 88(4), 497-500.
https://doi.org/10.1139/Y10-020

Ajdzanović VZ, Spasojević I, Filipović BR, Sosic-Jurjević BT, Sekulic MI, Milošević VL. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500.
doi:10.1139/Y10-020 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Filipović, Branko R, Sosic-Jurjević, Branka T, Sekulic, Milka I, Milošević, Verica Lj., "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500,
https://doi.org/10.1139/Y10-020 . .

TY  - JOUR
AU  - Spasojević, Ivan
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/425
AB  - EPR, free radicals, oxidative status, oxidative stress, antioxidants Kratak sadržaj: U patofiziološkim uslovima povezanim sa oksidativnim stresom, primenjivanje određenih antioksidativnih materija može biti od koristi za ljudsko zdravlje. Elektronska paramagnetna rezonantna (EPR) spektroskopija predstavlja tehniku koja pruža jedinstveni uvid u biohemijske redoks procese, zahvaljujući svom kapacitetu da: (i) razlikuje i kvantifikuje različite reaktivne vrste, kao što su hidroksil radikal, superoksid, ugljenični radikali, vodonični atom, azot monoksid, askorbil radikal, melanin i druge; (ii) odredi antioksidativne kapacitete različitih jedinjenje, ekstrakata i namirnica; (iii) pruži informacije o drugim važnim parametrima bioloških sistema. Kombinacija EPR spektroskopije i tradicionalnih biohemijskih metoda predstavlja efikasno oruđe u ispitivanju mehanizama oboljenja i moguće antioksidativne terapije, pružajući kompletniji uvid u redoks procese u ljudskom organizmu.
AB  - In pathophysiological conditions related to oxidative stress, the application of selected antioxidants could have beneficial effects on human health. Electron paramagnetic resonance (EPR) spectroscopy is a technique that provides unique insight into the redox biochemistry, due to its ability to: (i) distinguish and quantify different reactive species, such as hydroxyl radical, superoxide, carbon centered radicals, hydrogen atom, nitric oxide, ascorbyl radical, melanin, and others; (ii) evaluate the antioxidative capacity of various compounds, extracts and foods; (iii) provide information on other important parameters of biological systems. A combination of EPR spectroscopy and traditional biochemical methods represents an efficient tool in the studies of disease mechanisms and antioxidative therapy prospects, providing a more complete view into the redox processes in the human organism.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Elektronska paramagnetna rezonanca - moćno oruđe medicinske biohemije u otkrivanju mehanizama oboljenja i mogućih tretmana
T1  - Electron paramagnetic resonance: A powerful tool of medical biochemistry in discovering mechanisms of disease and treatment prospects
EP  - 188
IS  - 3
SP  - 175
VL  - 29
DO  - 10.2478/v10011-010-0020-0
ER  - 

@article{
author = "Spasojević, Ivan",
year = "2010",
abstract = "EPR, free radicals, oxidative status, oxidative stress, antioxidants Kratak sadržaj: U patofiziološkim uslovima povezanim sa oksidativnim stresom, primenjivanje određenih antioksidativnih materija može biti od koristi za ljudsko zdravlje. Elektronska paramagnetna rezonantna (EPR) spektroskopija predstavlja tehniku koja pruža jedinstveni uvid u biohemijske redoks procese, zahvaljujući svom kapacitetu da: (i) razlikuje i kvantifikuje različite reaktivne vrste, kao što su hidroksil radikal, superoksid, ugljenični radikali, vodonični atom, azot monoksid, askorbil radikal, melanin i druge; (ii) odredi antioksidativne kapacitete različitih jedinjenje, ekstrakata i namirnica; (iii) pruži informacije o drugim važnim parametrima bioloških sistema. Kombinacija EPR spektroskopije i tradicionalnih biohemijskih metoda predstavlja efikasno oruđe u ispitivanju mehanizama oboljenja i moguće antioksidativne terapije, pružajući kompletniji uvid u redoks procese u ljudskom organizmu., In pathophysiological conditions related to oxidative stress, the application of selected antioxidants could have beneficial effects on human health. Electron paramagnetic resonance (EPR) spectroscopy is a technique that provides unique insight into the redox biochemistry, due to its ability to: (i) distinguish and quantify different reactive species, such as hydroxyl radical, superoxide, carbon centered radicals, hydrogen atom, nitric oxide, ascorbyl radical, melanin, and others; (ii) evaluate the antioxidative capacity of various compounds, extracts and foods; (iii) provide information on other important parameters of biological systems. A combination of EPR spectroscopy and traditional biochemical methods represents an efficient tool in the studies of disease mechanisms and antioxidative therapy prospects, providing a more complete view into the redox processes in the human organism.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Elektronska paramagnetna rezonanca - moćno oruđe medicinske biohemije u otkrivanju mehanizama oboljenja i mogućih tretmana, Electron paramagnetic resonance: A powerful tool of medical biochemistry in discovering mechanisms of disease and treatment prospects",
pages = "188-175",
number = "3",
volume = "29",
doi = "10.2478/v10011-010-0020-0"
}

Spasojević, I.. (2010). Elektronska paramagnetna rezonanca - moćno oruđe medicinske biohemije u otkrivanju mehanizama oboljenja i mogućih tretmana. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 29(3), 175-188.
https://doi.org/10.2478/v10011-010-0020-0

Spasojević I. Elektronska paramagnetna rezonanca - moćno oruđe medicinske biohemije u otkrivanju mehanizama oboljenja i mogućih tretmana. in Journal of Medical Biochemistry. 2010;29(3):175-188.
doi:10.2478/v10011-010-0020-0 .

Spasojević, Ivan, "Elektronska paramagnetna rezonanca - moćno oruđe medicinske biohemije u otkrivanju mehanizama oboljenja i mogućih tretmana" in Journal of Medical Biochemistry, 29, no. 3 (2010):175-188,
https://doi.org/10.2478/v10011-010-0020-0 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/410
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 86
IS  - 2
SP  - 81
VL  - 15
DO  - 10.1179/174329210X12650506623320
ER  - 

@article{
author = "Spasojević, Ivan and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško P and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "86-81",
number = "2",
volume = "15",
doi = "10.1179/174329210X12650506623320"
}

Spasojević, I., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D. P.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Taylor & Francis Ltd, Abingdon., 15(2), 81-86.
https://doi.org/10.1179/174329210X12650506623320

Spasojević I, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević DP, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):81-86.
doi:10.1179/174329210X12650506623320 .

Spasojević, Ivan, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško P, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010):81-86,
https://doi.org/10.1179/174329210X12650506623320 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana M
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/373
AB  - Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles
EP  - 1557
IS  - 18
SP  - 1550
VL  - 167
DO  - 10.1016/j.jplph.2010.05.026
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana M and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2010",
abstract = "Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles",
pages = "1557-1550",
number = "18",
volume = "167",
doi = "10.1016/j.jplph.2010.05.026"
}

Hadzi-Tasković Sukalović, V., Vuletic, M. M., Veljović-Jovanović, S.,& Vučinić, Ž.. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 167(18), 1550-1557.
https://doi.org/10.1016/j.jplph.2010.05.026

Hadzi-Tasković Sukalović V, Vuletic MM, Veljović-Jovanović S, Vučinić Ž. The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology. 2010;167(18):1550-1557.
doi:10.1016/j.jplph.2010.05.026 .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana M, Veljović-Jovanović, Sonja, Vučinić, Željko, "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles" in Journal of Plant Physiology, 167, no. 18 (2010):1550-1557,
https://doi.org/10.1016/j.jplph.2010.05.026 . .

TY  - JOUR
AU  - Kukavica, Biljana
AU  - Mojović, Miloš
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
AU  - Takahama, Umeo
AU  - Veljović-Jovanović, Sonja
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/368
AB  - The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.
PB  - Oxford Univ Press, Oxford
T2  - Plant and Cell Physiology
T1  - Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
EP  - 317
IS  - 2
SP  - 304
VL  - 50
DO  - 10.1093/pcp/pcn199
ER  - 

@article{
author = "Kukavica, Biljana and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk and Takahama, Umeo and Veljović-Jovanović, Sonja",
year = "2009",
abstract = "The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.",
publisher = "Oxford Univ Press, Oxford",
journal = "Plant and Cell Physiology",
title = "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production",
pages = "317-304",
number = "2",
volume = "50",
doi = "10.1093/pcp/pcn199"
}

Kukavica, B., Mojović, M., Vučinić, Ž., Maksimović, V., Takahama, U.,& Veljović-Jovanović, S.. (2009). Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology
Oxford Univ Press, Oxford., 50(2), 304-317.
https://doi.org/10.1093/pcp/pcn199

Kukavica B, Mojović M, Vučinić Ž, Maksimović V, Takahama U, Veljović-Jovanović S. Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology. 2009;50(2):304-317.
doi:10.1093/pcp/pcn199 .

Kukavica, Biljana, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, Takahama, Umeo, Veljović-Jovanović, Sonja, "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production" in Plant and Cell Physiology, 50, no. 2 (2009):304-317,
https://doi.org/10.1093/pcp/pcn199 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasic, Snežana D.
AU  - Jones, David R
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/321
AB  - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
EP  - 84
IS  - 1
SP  - 80
VL  - 344
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
pages = "84-80",
number = "1",
volume = "344",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Spasojević, Ivan
AU  - Miković, Zeljko
AU  - Mandic, Vesna
AU  - Cerović, Nikola
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/330
AB  - Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide
EP  - 18
IS  - 1
SP  - 14
VL  - 2
DO  - 10.4161/oxim.2.1.7705
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Spasojević, Ivan and Miković, Zeljko and Mandic, Vesna and Cerović, Nikola and Spasić, Mihajlo",
year = "2009",
abstract = "Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide",
pages = "18-14",
number = "1",
volume = "2",
doi = "10.4161/oxim.2.1.7705"
}

Bogdanović Pristov, J., Spasojević, I., Miković, Z., Mandic, V., Cerović, N.,& Spasić, M.. (2009). Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2(1), 14-18.
https://doi.org/10.4161/oxim.2.1.7705

Bogdanović Pristov J, Spasojević I, Miković Z, Mandic V, Cerović N, Spasić M. Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity. 2009;2(1):14-18.
doi:10.4161/oxim.2.1.7705 .

Bogdanović Pristov, Jelena, Spasojević, Ivan, Miković, Zeljko, Mandic, Vesna, Cerović, Nikola, Spasić, Mihajlo, "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide" in Oxidative Medicine and Cellular Longevity, 2, no. 1 (2009):14-18,
https://doi.org/10.4161/oxim.2.1.7705 . .

TY  - JOUR
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Cvetic, T.
AU  - Živanović, Branka  D.
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/370
AB  - Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - Alternative respiration of fungus Phycomyces blakesleeanus
EP  - 217
IS  - 3
SP  - 207
VL  - 95
DO  - 10.1007/s10482-008-9304-5
ER  - 

@article{
author = "Zivić, Miroslav and Zakrzewska, Joanna and Stanić, Marina and Cvetic, T. and Živanović, Branka  D.",
year = "2009",
abstract = "Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "Alternative respiration of fungus Phycomyces blakesleeanus",
pages = "217-207",
number = "3",
volume = "95",
doi = "10.1007/s10482-008-9304-5"
}

Zivić, M., Zakrzewska, J., Stanić, M., Cvetic, T.,& Živanović, Branka  D.. (2009). Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 95(3), 207-217.
https://doi.org/10.1007/s10482-008-9304-5

Zivić M, Zakrzewska J, Stanić M, Cvetic T, Živanović, Branka  D.. Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2009;95(3):207-217.
doi:10.1007/s10482-008-9304-5 .

Zivić, Miroslav, Zakrzewska, Joanna, Stanić, Marina, Cvetic, T., Živanović, Branka  D., "Alternative respiration of fungus Phycomyces blakesleeanus" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 95, no. 3 (2009):207-217,
https://doi.org/10.1007/s10482-008-9304-5 . .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica M.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momcilović, Miljana
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Marjanović, Žaklina
AU  - Stosic-Grujicic, Stanislava
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/347
AB  - Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo
EP  - 707
IS  - 5
SP  - 696
VL  - 61
DO  - 10.1080/01635580902898743
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica M. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momcilović, Miljana and Tufegdžić, Srđan J. and Maksimović, Vuk and Marjanović, Žaklina and Stosic-Grujicic, Stanislava",
year = "2009",
abstract = "Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo",
pages = "707-696",
number = "5",
volume = "61",
doi = "10.1080/01635580902898743"
}

Harhaji-Trajković, L. M., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momcilović, M., Tufegdžić, S. J., Maksimović, V., Marjanović, Ž.,& Stosic-Grujicic, S.. (2009). Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 61(5), 696-707.
https://doi.org/10.1080/01635580902898743

Harhaji-Trajković LM, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momcilović M, Tufegdžić SJ, Maksimović V, Marjanović Ž, Stosic-Grujicic S. Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal. 2009;61(5):696-707.
doi:10.1080/01635580902898743 .

Harhaji-Trajković, Ljubica M., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momcilović, Miljana, Tufegdžić, Srđan J., Maksimović, Vuk, Marjanović, Žaklina, Stosic-Grujicic, Stanislava, "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo" in Nutrition and Cancer-An International Journal, 61, no. 5 (2009):696-707,
https://doi.org/10.1080/01635580902898743 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Jelic, Svetislav
AU  - Zakrzewska, Joanna
AU  - Bačić, Goran
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/309
AB  - Various mechanisms have been proposed to account for chemotherapy related ischemia, but none of them can explain the available clinical data. In order to explore the possibility that the decreased ability of erythrocytes to deliver oxygen to the heart could be responsible for cardiotoxicity, we have performed an ex vivo and in vivo study of the effects of cisplatin/5-FU on erythrocytes, using a variety of biophysical techniques. Combining EPR and microscopy it was concluded that both cardiotoxic 5-FU and non-cardiotoxic cisplatin have similar effects on the erythrocyte membrane, thus eliminating those changes as a potential source of cardiotoxicity. On the contrary, P-31-NMR and polarography showed that the effects of these cytostatics on the intracellular milieu differ significantly. 5-FU provoked a pronounced decrease of the O-2 level in blood and affected the metabolism of phosphate compounds, while cisplatin had no such effects. When combined these two drugs showed synergistic effects, which matches the higher frequency of cardiotoxicity of the combination relative to the sole application of 5-FU. Preliminary results acquired on blood of patients receiving cisplatin/5-FU therapy verified observations obtained ex vivo. These results open a possibility of applying NMR in preclinical trials of new drugs in order to predict their ischemic potential.
PB  - MDPI, Basel
T2  - Molecules
T1  - Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia
EP  - 67
IS  - 1
SP  - 53
VL  - 14
DO  - 10.3390/molecules14010053
ER  - 

@article{
author = "Spasojević, Ivan and Jelic, Svetislav and Zakrzewska, Joanna and Bačić, Goran",
year = "2009",
abstract = "Various mechanisms have been proposed to account for chemotherapy related ischemia, but none of them can explain the available clinical data. In order to explore the possibility that the decreased ability of erythrocytes to deliver oxygen to the heart could be responsible for cardiotoxicity, we have performed an ex vivo and in vivo study of the effects of cisplatin/5-FU on erythrocytes, using a variety of biophysical techniques. Combining EPR and microscopy it was concluded that both cardiotoxic 5-FU and non-cardiotoxic cisplatin have similar effects on the erythrocyte membrane, thus eliminating those changes as a potential source of cardiotoxicity. On the contrary, P-31-NMR and polarography showed that the effects of these cytostatics on the intracellular milieu differ significantly. 5-FU provoked a pronounced decrease of the O-2 level in blood and affected the metabolism of phosphate compounds, while cisplatin had no such effects. When combined these two drugs showed synergistic effects, which matches the higher frequency of cardiotoxicity of the combination relative to the sole application of 5-FU. Preliminary results acquired on blood of patients receiving cisplatin/5-FU therapy verified observations obtained ex vivo. These results open a possibility of applying NMR in preclinical trials of new drugs in order to predict their ischemic potential.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia",
pages = "67-53",
number = "1",
volume = "14",
doi = "10.3390/molecules14010053"
}

Spasojević, I., Jelic, S., Zakrzewska, J.,& Bačić, G.. (2009). Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia. in Molecules
MDPI, Basel., 14(1), 53-67.
https://doi.org/10.3390/molecules14010053

Spasojević I, Jelic S, Zakrzewska J, Bačić G. Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia. in Molecules. 2009;14(1):53-67.
doi:10.3390/molecules14010053 .

Spasojević, Ivan, Jelic, Svetislav, Zakrzewska, Joanna, Bačić, Goran, "Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia" in Molecules, 14, no. 1 (2009):53-67,
https://doi.org/10.3390/molecules14010053 . .

TY  - JOUR
AU  - Oreščanin-Dušić, Zorana
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojicic, Ratko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/335
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
EP  - 54
IS  - 2
SP  - 48
VL  - 14
DO  - 10.1179/135100009X392476
ER  - 

@article{
author = "Oreščanin-Dušić, Zorana and Milovanović, Slobodan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojicic, Ratko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
pages = "54-48",
number = "2",
volume = "14",
doi = "10.1179/135100009X392476"
}

Oreščanin-Dušić, Z., Milovanović, S., Blagojević, D., Nikolić-Kokić, A., Radojicic, R., Spasojević, I.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report
Taylor & Francis Ltd, Abingdon., 14(2), 48-54.
https://doi.org/10.1179/135100009X392476

Oreščanin-Dušić Z, Milovanović S, Blagojević D, Nikolić-Kokić A, Radojicic R, Spasojević I, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):48-54.
doi:10.1179/135100009X392476 .

Oreščanin-Dušić, Zorana, Milovanović, Slobodan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojicic, Ratko, Spasojević, Ivan, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009):48-54,
https://doi.org/10.1179/135100009X392476 . .

TY  - JOUR
AU  - Živković, J.
AU  - Zeković, Z.
AU  - Mujić, I.
AU  - Tumbas, V.
AU  - Cvetković, D.
AU  - Spasojević, Ivan
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/325
AB  - Using electron paramagnetic resonance (EPR) spectroscopy, antioxidant properties of chestnut extracts have been investigated as a source of phenolic compounds. In addition to their high antioxidant activities against hydroxyl and organic free (DPPH) radicals, phenolics showed to be potent protectors of membranes from lipid peroxidation To the best Of Our knowledge, the ability of an antioxidant to overcome body's refractory response towards antioxidant supplementation has been examined for the first time. The water soluble extracts obtained from leaves, catkins, and outer brown peel of Castanea sativa Mill. showed high antioxidant activity in scavenging OH and DPPH radical All extracts, except for sweet chestnut catkins, showed the ability to protect liposomes from peroxidation Phenolic compounds, as active antioxidants, have the ability to enter and protect cell membranes from lipid peroxidation, thus overcoming the body's refractory response to the antioxidant supplements In the diet. It is shown that phenolics are easily accessible natural antioxidants that can be Used as food supplements or for the treatment of pathophysiological conditions related to oxidative stress.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts
EP  - 427
IS  - 4
SP  - 421
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_325
ER  - 

@article{
author = "Živković, J. and Zeković, Z. and Mujić, I. and Tumbas, V. and Cvetković, D. and Spasojević, Ivan",
year = "2009",
abstract = "Using electron paramagnetic resonance (EPR) spectroscopy, antioxidant properties of chestnut extracts have been investigated as a source of phenolic compounds. In addition to their high antioxidant activities against hydroxyl and organic free (DPPH) radicals, phenolics showed to be potent protectors of membranes from lipid peroxidation To the best Of Our knowledge, the ability of an antioxidant to overcome body's refractory response towards antioxidant supplementation has been examined for the first time. The water soluble extracts obtained from leaves, catkins, and outer brown peel of Castanea sativa Mill. showed high antioxidant activity in scavenging OH and DPPH radical All extracts, except for sweet chestnut catkins, showed the ability to protect liposomes from peroxidation Phenolic compounds, as active antioxidants, have the ability to enter and protect cell membranes from lipid peroxidation, thus overcoming the body's refractory response to the antioxidant supplements In the diet. It is shown that phenolics are easily accessible natural antioxidants that can be Used as food supplements or for the treatment of pathophysiological conditions related to oxidative stress.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts",
pages = "427-421",
number = "4",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_325"
}

Živković, J., Zeković, Z., Mujić, I., Tumbas, V., Cvetković, D.,& Spasojević, I.. (2009). Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts. in Food Technology and Biotechnology
University of Zagreb., 47(4), 421-427.
https://hdl.handle.net/21.15107/rcub_rimsi_325

Živković J, Zeković Z, Mujić I, Tumbas V, Cvetković D, Spasojević I. Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts. in Food Technology and Biotechnology. 2009;47(4):421-427.
https://hdl.handle.net/21.15107/rcub_rimsi_325 .

Živković, J., Zeković, Z., Mujić, I., Tumbas, V., Cvetković, D., Spasojević, Ivan, "Antioxidant Properties of Phenolics in Castanea sativa Mill. Extracts" in Food Technology and Biotechnology, 47, no. 4 (2009):421-427,
https://hdl.handle.net/21.15107/rcub_rimsi_325 .

TY  - CONF
AU  - Simonović, Jasna
AU  - Ignjatović, Aleksandar
AU  - Spasojević, Ivan
AU  - Daković, Marko
AU  - Mojović, Miloš
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2944
AB  - We studied the positive health effect of different chocolates (antioxidative effects on stable
free radicals, reactive oxygen species (ROS) and for prevention of lipid peroxidation). The
results show that all chocolates successfully remove •OH radicals, but only chocolates with
high cocoa content are also effective for of •O2
-. The capabilities for chocolate samples to
reduce organic radicals are shown to be positive for hydrophobic DPPH which was not the
case for hydrophilic Tempone. Only the chocolate samples with high cocoa content were
shown to prevent lipid peroxidation induced by Fenton reaction. Obtained results showed
that chocolates have diverse antioxidative effects which are not only dependant on the
content of cocoa. The other chocolate constituents like: sugar, polyphenols, cocoa butter,
emulsifier and other substances should also be considered for determining the positive
health effect of chocolates.
PB  - The Society of Physical Chemists of Serbia
C3  - 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Chocolate – A Bittersweet Antioxidant
EP  - 393
SP  - 391
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2944
ER  - 

@conference{
author = "Simonović, Jasna and Ignjatović, Aleksandar and Spasojević, Ivan and Daković, Marko and Mojović, Miloš",
year = "2008",
abstract = "We studied the positive health effect of different chocolates (antioxidative effects on stable
free radicals, reactive oxygen species (ROS) and for prevention of lipid peroxidation). The
results show that all chocolates successfully remove •OH radicals, but only chocolates with
high cocoa content are also effective for of •O2
-. The capabilities for chocolate samples to
reduce organic radicals are shown to be positive for hydrophobic DPPH which was not the
case for hydrophilic Tempone. Only the chocolate samples with high cocoa content were
shown to prevent lipid peroxidation induced by Fenton reaction. Obtained results showed
that chocolates have diverse antioxidative effects which are not only dependant on the
content of cocoa. The other chocolate constituents like: sugar, polyphenols, cocoa butter,
emulsifier and other substances should also be considered for determining the positive
health effect of chocolates.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "9th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Chocolate – A Bittersweet Antioxidant",
pages = "393-391",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2944"
}

Simonović, J., Ignjatović, A., Spasojević, I., Daković, M.,& Mojović, M.. (2008). Chocolate – A Bittersweet Antioxidant. in 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry
The Society of Physical Chemists of Serbia., 391-393.
https://hdl.handle.net/21.15107/rcub_rimsi_2944

Simonović J, Ignjatović A, Spasojević I, Daković M, Mojović M. Chocolate – A Bittersweet Antioxidant. in 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2008;:391-393.
https://hdl.handle.net/21.15107/rcub_rimsi_2944 .

Simonović, Jasna, Ignjatović, Aleksandar, Spasojević, Ivan, Daković, Marko, Mojović, Miloš, "Chocolate – A Bittersweet Antioxidant" in 9th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2008):391-393,
https://hdl.handle.net/21.15107/rcub_rimsi_2944 .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Živanović, Branka  D.
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/266
AB  - Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth
EP  - 662
IS  - 5
SP  - 656
VL  - 175
DO  - 10.1016/j.plantsci.2008.06.015
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Maksimović, Vuk and Živanović, Branka  D. and Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana",
year = "2008",
abstract = "Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth",
pages = "662-656",
number = "5",
volume = "175",
doi = "10.1016/j.plantsci.2008.06.015"
}

Dragišić Maksimović, J., Maksimović, V., Živanović, Branka  D., Hadzi-Tasković Sukalović, V.,& Vuletic, M.. (2008). Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science
Elsevier Ireland Ltd, Clare., 175(5), 656-662.
https://doi.org/10.1016/j.plantsci.2008.06.015

Dragišić Maksimović J, Maksimović V, Živanović, Branka  D., Hadzi-Tasković Sukalović V, Vuletic M. Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science. 2008;175(5):656-662.
doi:10.1016/j.plantsci.2008.06.015 .

Dragišić Maksimović, Jelena, Maksimović, Vuk, Živanović, Branka  D., Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth" in Plant Science, 175, no. 5 (2008):656-662,
https://doi.org/10.1016/j.plantsci.2008.06.015 . .

TY  - JOUR
AU  - Todorović, Sladana
AU  - Giba, Zlatko
AU  - Bačić, Goran
AU  - Nikolic, Miroslav
AU  - Grubisic, Dragoljub
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/299
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
EP  - 324
IS  - 3
SP  - 322
VL  - 64
DO  - 10.1016/j.envexpbot.2008.07.001
ER  - 

@article{
author = "Todorović, Sladana and Giba, Zlatko and Bačić, Goran and Nikolic, Miroslav and Grubisic, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
pages = "324-322",
number = "3",
volume = "64",
doi = "10.1016/j.envexpbot.2008.07.001"
}

Todorović, S., Giba, Z., Bačić, G., Nikolic, M.,& Grubisic, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 64(3), 322-324.
https://doi.org/10.1016/j.envexpbot.2008.07.001

Todorović S, Giba Z, Bačić G, Nikolic M, Grubisic D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):322-324.
doi:10.1016/j.envexpbot.2008.07.001 .

Todorović, Sladana, Giba, Zlatko, Bačić, Goran, Nikolic, Miroslav, Grubisic, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008):322-324,
https://doi.org/10.1016/j.envexpbot.2008.07.001 . .