TY  - JOUR
AU  - Vuletic, Mirjana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Marković, Ksenija
AU  - Kravić, Natalija B
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/810
AB  - An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.
PB  - Wiley, Hoboken
T2  - Plant Biology
T1  - Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress
EP  - 96
IS  - 1
SP  - 88
VL  - 16
DO  - 10.1111/plb.12017
ER  - 

@article{
author = "Vuletic, Mirjana M and Hadzi-Tasković Sukalović, Vesna and Marković, Ksenija and Kravić, Natalija B and Vučinić, Željko and Maksimović, Vuk",
year = "2014",
abstract = "An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.",
publisher = "Wiley, Hoboken",
journal = "Plant Biology",
title = "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress",
pages = "96-88",
number = "1",
volume = "16",
doi = "10.1111/plb.12017"
}

Vuletic, M. M., Hadzi-Tasković Sukalović, V., Marković, K., Kravić, N. B., Vučinić, Ž.,& Maksimović, V.. (2014). Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. in Plant Biology
Wiley, Hoboken., 16(1), 88-96.
https://doi.org/10.1111/plb.12017

Vuletic MM, Hadzi-Tasković Sukalović V, Marković K, Kravić NB, Vučinić Ž, Maksimović V. Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. in Plant Biology. 2014;16(1):88-96.
doi:10.1111/plb.12017 .

Vuletic, Mirjana M, Hadzi-Tasković Sukalović, Vesna, Marković, Ksenija, Kravić, Natalija B, Vučinić, Željko, Maksimović, Vuk, "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress" in Plant Biology, 16, no. 1 (2014):88-96,
https://doi.org/10.1111/plb.12017 . .

TY  - JOUR
AU  - Zilic, Slađana M
AU  - Basic, Zorica
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Maksimović, Vuk
AU  - Janković, Marijana Z
AU  - Filipović, Milomir
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/758
AB  - The content of tocopherols, B vitamins, as well as free soluble, soluble conjugated and insoluble bound phenolic compounds was determined in untreated, steeped and sprouted wheat grains. Antioxidant capacity of whole wheat grains and their phenolic fractions was also evaluated. Sprouting significantly increased the levels of tocopherols, niacin, riboflavin, as well as free and bound phenolic compounds improving nutritional value and antioxidant capacity of wheat grains/flour. After sprouting for 5days, the content of total phenolics, flavonoids and ferulic acid calculated as the sum of its fractions was increased by 9.9, 30.7 and 21.6%, respectively. The content of -, +- and -tocopherols was increased for 3.59-fold, 2.33-fold and 2.61-fold respectively, while the content of niacin, as predominant B vitamin, was increased for 1.19-fold after sprouting. The total antioxidant capacity of untreated, steeped and sprouted whole wheat grains was 19.44, 20.37 and 22.70mmol Trolox Eq/kg, respectively. Sprouted wheat, as a rich source of bioavailable phytochemicals, should be used to improve the nutritional quality of food.
PB  - Wiley, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Can the sprouting process applied to wheat improve the contents of vitamins and phenolic compounds and antioxidant capacity of the flour?
EP  - 1047
IS  - 4
SP  - 1040
VL  - 49
DO  - 10.1111/ijfs.12397
ER  - 

@article{
author = "Zilic, Slađana M and Basic, Zorica and Hadzi-Tasković Sukalović, Vesna and Maksimović, Vuk and Janković, Marijana Z and Filipović, Milomir",
year = "2014",
abstract = "The content of tocopherols, B vitamins, as well as free soluble, soluble conjugated and insoluble bound phenolic compounds was determined in untreated, steeped and sprouted wheat grains. Antioxidant capacity of whole wheat grains and their phenolic fractions was also evaluated. Sprouting significantly increased the levels of tocopherols, niacin, riboflavin, as well as free and bound phenolic compounds improving nutritional value and antioxidant capacity of wheat grains/flour. After sprouting for 5days, the content of total phenolics, flavonoids and ferulic acid calculated as the sum of its fractions was increased by 9.9, 30.7 and 21.6%, respectively. The content of -, +- and -tocopherols was increased for 3.59-fold, 2.33-fold and 2.61-fold respectively, while the content of niacin, as predominant B vitamin, was increased for 1.19-fold after sprouting. The total antioxidant capacity of untreated, steeped and sprouted whole wheat grains was 19.44, 20.37 and 22.70mmol Trolox Eq/kg, respectively. Sprouted wheat, as a rich source of bioavailable phytochemicals, should be used to improve the nutritional quality of food.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Can the sprouting process applied to wheat improve the contents of vitamins and phenolic compounds and antioxidant capacity of the flour?",
pages = "1047-1040",
number = "4",
volume = "49",
doi = "10.1111/ijfs.12397"
}

Zilic, S. M., Basic, Z., Hadzi-Tasković Sukalović, V., Maksimović, V., Janković, M. Z.,& Filipović, M.. (2014). Can the sprouting process applied to wheat improve the contents of vitamins and phenolic compounds and antioxidant capacity of the flour?. in International Journal of Food Science and Technology
Wiley, Hoboken., 49(4), 1040-1047.
https://doi.org/10.1111/ijfs.12397

Zilic SM, Basic Z, Hadzi-Tasković Sukalović V, Maksimović V, Janković MZ, Filipović M. Can the sprouting process applied to wheat improve the contents of vitamins and phenolic compounds and antioxidant capacity of the flour?. in International Journal of Food Science and Technology. 2014;49(4):1040-1047.
doi:10.1111/ijfs.12397 .

Zilic, Slađana M, Basic, Zorica, Hadzi-Tasković Sukalović, Vesna, Maksimović, Vuk, Janković, Marijana Z, Filipović, Milomir, "Can the sprouting process applied to wheat improve the contents of vitamins and phenolic compounds and antioxidant capacity of the flour?" in International Journal of Food Science and Technology, 49, no. 4 (2014):1040-1047,
https://doi.org/10.1111/ijfs.12397 . .

TY  - JOUR
AU  - Zilic, Slađana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Dodig, Dejan
AU  - Maksimović, Vuk
AU  - Kandic, Vesna
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/633
AB  - The objective of this study was to determine phenolic compounds and the total antioxidant capacity in the grain of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) wheat genotypes. Soluble free forms of total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids were investigated. In addition, the correlation coefficients between total antioxidant capacities and the concentration of different soluble free phenolic compounds, as well as between soluble free total phenolics and phenolic acids, flavonoids and PVPP bound phenolics were determined. Significant differences in the content of aceton/water extractable total phenolics, PVPP bound phenolics and phenolic acids between and within two wheat species were found. On the average, durum wheat samples had about 1.19-fold higher total phenolic compounds and about 1.5-fold higher PVPP bound phenolics than bread wheat samples. Three phenolic acids, ferulic, caffeic and chlorogenic, were detected in wholemeal bread wheat. Caffeic acid was not found in durum wheat samples whilst ferulic acid was the most abundant. Proanthocyanidins in bread and durum wheat genotypes were not detected. The antioxidant capacity measured as the DPPH radical scavenging activity was similar in wholemeal of bread and durum wheat, however, significant differences were observed among genotypes within species.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes
EP  - 100
IS  - 1
SP  - 87
VL  - 45
DO  - 10.2298/GENSR1301087Z
ER  - 

@article{
author = "Zilic, Slađana M and Hadzi-Tasković Sukalović, Vesna and Dodig, Dejan and Maksimović, Vuk and Kandic, Vesna",
year = "2013",
abstract = "The objective of this study was to determine phenolic compounds and the total antioxidant capacity in the grain of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) wheat genotypes. Soluble free forms of total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids were investigated. In addition, the correlation coefficients between total antioxidant capacities and the concentration of different soluble free phenolic compounds, as well as between soluble free total phenolics and phenolic acids, flavonoids and PVPP bound phenolics were determined. Significant differences in the content of aceton/water extractable total phenolics, PVPP bound phenolics and phenolic acids between and within two wheat species were found. On the average, durum wheat samples had about 1.19-fold higher total phenolic compounds and about 1.5-fold higher PVPP bound phenolics than bread wheat samples. Three phenolic acids, ferulic, caffeic and chlorogenic, were detected in wholemeal bread wheat. Caffeic acid was not found in durum wheat samples whilst ferulic acid was the most abundant. Proanthocyanidins in bread and durum wheat genotypes were not detected. The antioxidant capacity measured as the DPPH radical scavenging activity was similar in wholemeal of bread and durum wheat, however, significant differences were observed among genotypes within species.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes",
pages = "100-87",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301087Z"
}

Zilic, S. M., Hadzi-Tasković Sukalović, V., Dodig, D., Maksimović, V.,& Kandic, V.. (2013). Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 87-100.
https://doi.org/10.2298/GENSR1301087Z

Zilic SM, Hadzi-Tasković Sukalović V, Dodig D, Maksimović V, Kandic V. Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes. in Genetika-Belgrade. 2013;45(1):87-100.
doi:10.2298/GENSR1301087Z .

Zilic, Slađana M, Hadzi-Tasković Sukalović, Vesna, Dodig, Dejan, Maksimović, Vuk, Kandic, Vesna, "Soluble free phenolic compound contents and antioxidant capacity of bread and durum wheat genotypes" in Genetika-Belgrade, 45, no. 1 (2013):87-100,
https://doi.org/10.2298/GENSR1301087Z . .

TY  - JOUR
AU  - Zilic, Slađana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Dodig, Dejan
AU  - Maksimović, Vuk
AU  - Maksimović, Milan
AU  - Basic, Zorica
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/461
AB  - In this study, the content of soluble, free forms of phenolic compounds (total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids), as well as the content of carotenoids and tocopherols, were determined in whole grains of bread and durum wheat, rye, hull-less barley and hull-less oat, each represented with four genotypes. Antioxidant activity was evaluated as radical scavenging activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, as well as by hydrogen transfer reaction (reduction power) based on the reduction of Fe3+. Generally, a considerable variation in antioxidant activities and phytochemical contents was observed between the cereals. Remarkably higher DPPH radical scavenging ability and reducing power were detected in hull-less barley, followed by rye and hull-less oat and durum and bread wheat, indicating that small grain species have different major antioxidants with different properties. Hull-less barley had the highest content of total free phenols, flavonoids, PVPP bound phenolics and contained flavan-3-ols, not found in other species. Hull-less oat had the highest content of tocopherols, very high content of yellow pigments and PVPP bound phenolics. Ferulic acid was the major free phenolic acid in small grain cereals tested. The relationship between the content of soluble phenols, as well as reducing power and DPPH scavenging activity are also considered.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Cereal Science
T1  - Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants
EP  - 424
IS  - 3
SP  - 417
VL  - 54
DO  - 10.1016/j.jcs.2011.08.006
ER  - 

@article{
author = "Zilic, Slađana M and Hadzi-Tasković Sukalović, Vesna and Dodig, Dejan and Maksimović, Vuk and Maksimović, Milan and Basic, Zorica",
year = "2011",
abstract = "In this study, the content of soluble, free forms of phenolic compounds (total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids), as well as the content of carotenoids and tocopherols, were determined in whole grains of bread and durum wheat, rye, hull-less barley and hull-less oat, each represented with four genotypes. Antioxidant activity was evaluated as radical scavenging activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, as well as by hydrogen transfer reaction (reduction power) based on the reduction of Fe3+. Generally, a considerable variation in antioxidant activities and phytochemical contents was observed between the cereals. Remarkably higher DPPH radical scavenging ability and reducing power were detected in hull-less barley, followed by rye and hull-less oat and durum and bread wheat, indicating that small grain species have different major antioxidants with different properties. Hull-less barley had the highest content of total free phenols, flavonoids, PVPP bound phenolics and contained flavan-3-ols, not found in other species. Hull-less oat had the highest content of tocopherols, very high content of yellow pigments and PVPP bound phenolics. Ferulic acid was the major free phenolic acid in small grain cereals tested. The relationship between the content of soluble phenols, as well as reducing power and DPPH scavenging activity are also considered.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Cereal Science",
title = "Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants",
pages = "424-417",
number = "3",
volume = "54",
doi = "10.1016/j.jcs.2011.08.006"
}

Zilic, S. M., Hadzi-Tasković Sukalović, V., Dodig, D., Maksimović, V., Maksimović, M.,& Basic, Z.. (2011). Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants. in Journal of Cereal Science
Academic Press Ltd- Elsevier Science Ltd, London., 54(3), 417-424.
https://doi.org/10.1016/j.jcs.2011.08.006

Zilic SM, Hadzi-Tasković Sukalović V, Dodig D, Maksimović V, Maksimović M, Basic Z. Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants. in Journal of Cereal Science. 2011;54(3):417-424.
doi:10.1016/j.jcs.2011.08.006 .

Zilic, Slađana M, Hadzi-Tasković Sukalović, Vesna, Dodig, Dejan, Maksimović, Vuk, Maksimović, Milan, Basic, Zorica, "Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants" in Journal of Cereal Science, 54, no. 3 (2011):417-424,
https://doi.org/10.1016/j.jcs.2011.08.006 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Veljović-Jovanović, Sonja
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Pajic, Zorica
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/399
AB  - Silk of some maize genotypes contains a high level of phenolics that undergo enzymatic oxidation to form quinones, which condense among themselves or with proteins to form brown pigments. Two phenolic oxidizing enzymes, peroxidase (POD; EC 1.11.1.7) and polyphenol oxidase (PPO; EC 1.10.3.1), from maize (Zea mays L.) silk were characterised with respect to their preferred substrate, different isoforms and specific effectors. One browning silk sample with high, and two non-browning samples with low phenolic content were investigated. Although POD oxidizes a wide range of phenolic substrates in vitro, its activity rate was independent of silk phenolic content. PPO activity, detected with o-diphenolic substrates, was abundant only in browning silk, and low or absent in non-browning silk. Pollination increased POD but not PPO activity. Isoelectric-focusing (IEF) and specific staining for POD and PPO showed a high degree of polymorphism that varied with silk origin. The IEF pattern of POD revealed a number of anionic and several cationic isoenzymes, with the most pronounced having neutral pI 7 and a basic isoform with pI 10. Detected isoforms of PPO were anionic, except for one neutral form found only in browning silk, and occupied positions different from those of POD. Different inhibitory effects of NaN3, EDTA, KCN, and L-cysteine, as well as different impacts of a variety of cations on the oxidation of chlorogenic acid, mediated by PPO or POD, were detected. The findings are discussed in terms of a possible roles of these enzymes in defence and pollination.
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Biology
T1  - Characterisation of phenol oxidase and peroxidase from maize silk
EP  - 413
IS  - 3
SP  - 406
VL  - 12
DO  - 10.1111/j.1438-8677.2009.00237.x
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Veljović-Jovanović, Sonja and Dragišić Maksimović, Jelena and Maksimović, Vuk and Pajic, Zorica",
year = "2010",
abstract = "Silk of some maize genotypes contains a high level of phenolics that undergo enzymatic oxidation to form quinones, which condense among themselves or with proteins to form brown pigments. Two phenolic oxidizing enzymes, peroxidase (POD; EC 1.11.1.7) and polyphenol oxidase (PPO; EC 1.10.3.1), from maize (Zea mays L.) silk were characterised with respect to their preferred substrate, different isoforms and specific effectors. One browning silk sample with high, and two non-browning samples with low phenolic content were investigated. Although POD oxidizes a wide range of phenolic substrates in vitro, its activity rate was independent of silk phenolic content. PPO activity, detected with o-diphenolic substrates, was abundant only in browning silk, and low or absent in non-browning silk. Pollination increased POD but not PPO activity. Isoelectric-focusing (IEF) and specific staining for POD and PPO showed a high degree of polymorphism that varied with silk origin. The IEF pattern of POD revealed a number of anionic and several cationic isoenzymes, with the most pronounced having neutral pI 7 and a basic isoform with pI 10. Detected isoforms of PPO were anionic, except for one neutral form found only in browning silk, and occupied positions different from those of POD. Different inhibitory effects of NaN3, EDTA, KCN, and L-cysteine, as well as different impacts of a variety of cations on the oxidation of chlorogenic acid, mediated by PPO or POD, were detected. The findings are discussed in terms of a possible roles of these enzymes in defence and pollination.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Biology",
title = "Characterisation of phenol oxidase and peroxidase from maize silk",
pages = "413-406",
number = "3",
volume = "12",
doi = "10.1111/j.1438-8677.2009.00237.x"
}

Hadzi-Tasković Sukalović, V., Veljović-Jovanović, S., Dragišić Maksimović, J., Maksimović, V.,& Pajic, Z.. (2010). Characterisation of phenol oxidase and peroxidase from maize silk. in Plant Biology
Wiley-Blackwell, Hoboken., 12(3), 406-413.
https://doi.org/10.1111/j.1438-8677.2009.00237.x

Hadzi-Tasković Sukalović V, Veljović-Jovanović S, Dragišić Maksimović J, Maksimović V, Pajic Z. Characterisation of phenol oxidase and peroxidase from maize silk. in Plant Biology. 2010;12(3):406-413.
doi:10.1111/j.1438-8677.2009.00237.x .

Hadzi-Tasković Sukalović, Vesna, Veljović-Jovanović, Sonja, Dragišić Maksimović, Jelena, Maksimović, Vuk, Pajic, Zorica, "Characterisation of phenol oxidase and peroxidase from maize silk" in Plant Biology, 12, no. 3 (2010):406-413,
https://doi.org/10.1111/j.1438-8677.2009.00237.x . .

TY  - JOUR
AU  - Vuletic, Mirjana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Marković, Ksenija
AU  - Dragišić Maksimović, Jelena
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/390
AB  - The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
EP  - 534
IS  - 3
SP  - 530
VL  - 54
DO  - 10.1007/s10535-010-0093-0
ER  - 

@article{
author = "Vuletic, Mirjana M and Hadzi-Tasković Sukalović, Vesna and Marković, Ksenija and Dragišić Maksimović, Jelena",
year = "2010",
abstract = "The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources",
pages = "534-530",
number = "3",
volume = "54",
doi = "10.1007/s10535-010-0093-0"
}

Vuletic, M. M., Hadzi-Tasković Sukalović, V., Marković, K.,& Dragišić Maksimović, J.. (2010). Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. in Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 54(3), 530-534.
https://doi.org/10.1007/s10535-010-0093-0

Vuletic MM, Hadzi-Tasković Sukalović V, Marković K, Dragišić Maksimović J. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. in Biologia Plantarum. 2010;54(3):530-534.
doi:10.1007/s10535-010-0093-0 .

Vuletic, Mirjana M, Hadzi-Tasković Sukalović, Vesna, Marković, Ksenija, Dragišić Maksimović, Jelena, "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources" in Biologia Plantarum, 54, no. 3 (2010):530-534,
https://doi.org/10.1007/s10535-010-0093-0 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana M
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/373
AB  - Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles
EP  - 1557
IS  - 18
SP  - 1550
VL  - 167
DO  - 10.1016/j.jplph.2010.05.026
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana M and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2010",
abstract = "Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles",
pages = "1557-1550",
number = "18",
volume = "167",
doi = "10.1016/j.jplph.2010.05.026"
}

Hadzi-Tasković Sukalović, V., Vuletic, M. M., Veljović-Jovanović, S.,& Vučinić, Ž.. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 167(18), 1550-1557.
https://doi.org/10.1016/j.jplph.2010.05.026

Hadzi-Tasković Sukalović V, Vuletic MM, Veljović-Jovanović S, Vučinić Ž. The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology. 2010;167(18):1550-1557.
doi:10.1016/j.jplph.2010.05.026 .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana M, Veljović-Jovanović, Sonja, Vučinić, Željko, "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles" in Journal of Plant Physiology, 167, no. 18 (2010):1550-1557,
https://doi.org/10.1016/j.jplph.2010.05.026 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Živanović, Branka  D.
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/266
AB  - Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth
EP  - 662
IS  - 5
SP  - 656
VL  - 175
DO  - 10.1016/j.plantsci.2008.06.015
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Maksimović, Vuk and Živanović, Branka  D. and Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana",
year = "2008",
abstract = "Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth",
pages = "662-656",
number = "5",
volume = "175",
doi = "10.1016/j.plantsci.2008.06.015"
}

Dragišić Maksimović, J., Maksimović, V., Živanović, Branka  D., Hadzi-Tasković Sukalović, V.,& Vuletic, M.. (2008). Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science
Elsevier Ireland Ltd, Clare., 175(5), 656-662.
https://doi.org/10.1016/j.plantsci.2008.06.015

Dragišić Maksimović J, Maksimović V, Živanović, Branka  D., Hadzi-Tasković Sukalović V, Vuletic M. Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science. 2008;175(5):656-662.
doi:10.1016/j.plantsci.2008.06.015 .

Dragišić Maksimović, Jelena, Maksimović, Vuk, Živanović, Branka  D., Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth" in Plant Science, 175, no. 5 (2008):656-662,
https://doi.org/10.1016/j.plantsci.2008.06.015 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
AU  - Vučinić, Željko
AU  - Veljović-Jovanović, Sonja
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/264
AB  - The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.
PB  - Springer Japan Kk, Tokyo
T2  - Journal of Plant Research
T1  - Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH
EP  - 123
IS  - 1
SP  - 115
VL  - 121
DO  - 10.1007/s10265-007-0124-x
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana and Vučinić, Željko and Veljović-Jovanović, Sonja",
year = "2008",
abstract = "The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.",
publisher = "Springer Japan Kk, Tokyo",
journal = "Journal of Plant Research",
title = "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH",
pages = "123-115",
number = "1",
volume = "121",
doi = "10.1007/s10265-007-0124-x"
}

Hadzi-Tasković Sukalović, V., Vuletic, M., Vučinić, Ž.,& Veljović-Jovanović, S.. (2008). Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research
Springer Japan Kk, Tokyo., 121(1), 115-123.
https://doi.org/10.1007/s10265-007-0124-x

Hadzi-Tasković Sukalović V, Vuletic M, Vučinić Ž, Veljović-Jovanović S. Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research. 2008;121(1):115-123.
doi:10.1007/s10265-007-0124-x .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, Vučinić, Željko, Veljović-Jovanović, Sonja, "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH" in Journal of Plant Research, 121, no. 1 (2008):115-123,
https://doi.org/10.1007/s10265-007-0124-x . .