TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Ignjatović, Aleksandar
AU  - Bačić, Goran
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/503
AB  - In this era of intense study of free radicals and antioxidants, electron paramagnetic resonance (EPR) is arguably the best-suited technique for such research, particularly when considering biochemical and biological systems. No attempt was made to cover all the topics of EPR application but instead attention was restricted to two areas that are both novel and received less attention in previous reviews. In the first section, the application of EPR in assessing the oxidative status of various biological systems, using endogenous stabile paramagnetic species, such as the ascorbyl radical, semiquinone, melanin, and oxidized pigments, is addressed. The second section covers the use of EPR in the emerging field of antioxidant development, using EPR spin-trapping and spin-probing techniques. In both sections, in addition to giving an overview of the available literature, examples (mostly from the authors' recent work) are also presented in sufficient detail to illustrate how to explore the full potential of EPR. This review aims at encouraging biologists, chemists and pharmacologists interested in the redox metabolism of living systems, free radical chemistry or antioxidative properties of new drugs and natural products to take advantage of this technique for their investigations.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds
EP  - 677
IS  - 5
SP  - 647
VL  - 76
DO  - 10.2298/JSC101015064S
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Ignjatović, Aleksandar and Bačić, Goran",
year = "2011",
abstract = "In this era of intense study of free radicals and antioxidants, electron paramagnetic resonance (EPR) is arguably the best-suited technique for such research, particularly when considering biochemical and biological systems. No attempt was made to cover all the topics of EPR application but instead attention was restricted to two areas that are both novel and received less attention in previous reviews. In the first section, the application of EPR in assessing the oxidative status of various biological systems, using endogenous stabile paramagnetic species, such as the ascorbyl radical, semiquinone, melanin, and oxidized pigments, is addressed. The second section covers the use of EPR in the emerging field of antioxidant development, using EPR spin-trapping and spin-probing techniques. In both sections, in addition to giving an overview of the available literature, examples (mostly from the authors' recent work) are also presented in sufficient detail to illustrate how to explore the full potential of EPR. This review aims at encouraging biologists, chemists and pharmacologists interested in the redox metabolism of living systems, free radical chemistry or antioxidative properties of new drugs and natural products to take advantage of this technique for their investigations.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds",
pages = "677-647",
number = "5",
volume = "76",
doi = "10.2298/JSC101015064S"
}

Spasojević, I., Mojović, M., Ignjatović, A.,& Bačić, G.. (2011). The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 76(5), 647-677.
https://doi.org/10.2298/JSC101015064S

Spasojević I, Mojović M, Ignjatović A, Bačić G. The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds. in Journal of the Serbian Chemical Society. 2011;76(5):647-677.
doi:10.2298/JSC101015064S .

Spasojević, Ivan, Mojović, Miloš, Ignjatović, Aleksandar, Bačić, Goran, "The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds" in Journal of the Serbian Chemical Society, 76, no. 5 (2011):647-677,
https://doi.org/10.2298/JSC101015064S . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Jelic, Svetislav
AU  - Zakrzewska, Joanna
AU  - Bačić, Goran
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/309
AB  - Various mechanisms have been proposed to account for chemotherapy related ischemia, but none of them can explain the available clinical data. In order to explore the possibility that the decreased ability of erythrocytes to deliver oxygen to the heart could be responsible for cardiotoxicity, we have performed an ex vivo and in vivo study of the effects of cisplatin/5-FU on erythrocytes, using a variety of biophysical techniques. Combining EPR and microscopy it was concluded that both cardiotoxic 5-FU and non-cardiotoxic cisplatin have similar effects on the erythrocyte membrane, thus eliminating those changes as a potential source of cardiotoxicity. On the contrary, P-31-NMR and polarography showed that the effects of these cytostatics on the intracellular milieu differ significantly. 5-FU provoked a pronounced decrease of the O-2 level in blood and affected the metabolism of phosphate compounds, while cisplatin had no such effects. When combined these two drugs showed synergistic effects, which matches the higher frequency of cardiotoxicity of the combination relative to the sole application of 5-FU. Preliminary results acquired on blood of patients receiving cisplatin/5-FU therapy verified observations obtained ex vivo. These results open a possibility of applying NMR in preclinical trials of new drugs in order to predict their ischemic potential.
PB  - MDPI, Basel
T2  - Molecules
T1  - Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia
EP  - 67
IS  - 1
SP  - 53
VL  - 14
DO  - 10.3390/molecules14010053
ER  - 

@article{
author = "Spasojević, Ivan and Jelic, Svetislav and Zakrzewska, Joanna and Bačić, Goran",
year = "2009",
abstract = "Various mechanisms have been proposed to account for chemotherapy related ischemia, but none of them can explain the available clinical data. In order to explore the possibility that the decreased ability of erythrocytes to deliver oxygen to the heart could be responsible for cardiotoxicity, we have performed an ex vivo and in vivo study of the effects of cisplatin/5-FU on erythrocytes, using a variety of biophysical techniques. Combining EPR and microscopy it was concluded that both cardiotoxic 5-FU and non-cardiotoxic cisplatin have similar effects on the erythrocyte membrane, thus eliminating those changes as a potential source of cardiotoxicity. On the contrary, P-31-NMR and polarography showed that the effects of these cytostatics on the intracellular milieu differ significantly. 5-FU provoked a pronounced decrease of the O-2 level in blood and affected the metabolism of phosphate compounds, while cisplatin had no such effects. When combined these two drugs showed synergistic effects, which matches the higher frequency of cardiotoxicity of the combination relative to the sole application of 5-FU. Preliminary results acquired on blood of patients receiving cisplatin/5-FU therapy verified observations obtained ex vivo. These results open a possibility of applying NMR in preclinical trials of new drugs in order to predict their ischemic potential.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia",
pages = "67-53",
number = "1",
volume = "14",
doi = "10.3390/molecules14010053"
}

Spasojević, I., Jelic, S., Zakrzewska, J.,& Bačić, G.. (2009). Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia. in Molecules
MDPI, Basel., 14(1), 53-67.
https://doi.org/10.3390/molecules14010053

Spasojević I, Jelic S, Zakrzewska J, Bačić G. Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia. in Molecules. 2009;14(1):53-67.
doi:10.3390/molecules14010053 .

Spasojević, Ivan, Jelic, Svetislav, Zakrzewska, Joanna, Bačić, Goran, "Decreased Oxygen Transfer Capacity of Erythrocytes as a Cause of 5-Fluorouracil Related Ischemia" in Molecules, 14, no. 1 (2009):53-67,
https://doi.org/10.3390/molecules14010053 . .

TY  - JOUR
AU  - Todorović, Sladana
AU  - Giba, Zlatko
AU  - Bačić, Goran
AU  - Nikolic, Miroslav
AU  - Grubisic, Dragoljub
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/299
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
EP  - 324
IS  - 3
SP  - 322
VL  - 64
DO  - 10.1016/j.envexpbot.2008.07.001
ER  - 

@article{
author = "Todorović, Sladana and Giba, Zlatko and Bačić, Goran and Nikolic, Miroslav and Grubisic, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
pages = "324-322",
number = "3",
volume = "64",
doi = "10.1016/j.envexpbot.2008.07.001"
}

Todorović, S., Giba, Z., Bačić, G., Nikolic, M.,& Grubisic, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 64(3), 322-324.
https://doi.org/10.1016/j.envexpbot.2008.07.001

Todorović S, Giba Z, Bačić G, Nikolic M, Grubisic D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):322-324.
doi:10.1016/j.envexpbot.2008.07.001 .

Todorović, Sladana, Giba, Zlatko, Bačić, Goran, Nikolic, Miroslav, Grubisic, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008):322-324,
https://doi.org/10.1016/j.envexpbot.2008.07.001 . .

TY  - JOUR
AU  - Bačić, Goran
AU  - Spasojević, Ivan
AU  - Secerov, Bojana
AU  - Mojović, Miloš
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/287
AB  - The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1  - Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities
EP  - 1366
IS  - 5
SP  - 1354
VL  - 69
DO  - 10.1016/j.saa.2007.09.047
ER  - 

@article{
author = "Bačić, Goran and Spasojević, Ivan and Secerov, Bojana and Mojović, Miloš",
year = "2008",
abstract = "The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities",
pages = "1366-1354",
number = "5",
volume = "69",
doi = "10.1016/j.saa.2007.09.047"
}

Bačić, G., Spasojević, I., Secerov, B.,& Mojović, M.. (2008). Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 69(5), 1354-1366.
https://doi.org/10.1016/j.saa.2007.09.047

Bačić G, Spasojević I, Secerov B, Mojović M. Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2008;69(5):1354-1366.
doi:10.1016/j.saa.2007.09.047 .

Bačić, Goran, Spasojević, Ivan, Secerov, Bojana, Mojović, Miloš, "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 69, no. 5 (2008):1354-1366,
https://doi.org/10.1016/j.saa.2007.09.047 . .

TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
AU  - Bačić, Goran
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/167
PB  - Taylor & Francis Ltd, Abingdon
T2  - Free Radical Research
T1  - Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems
EP  - S72
SP  - S72
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_167
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Spasić, Mihajlo and Bačić, Goran",
year = "2006",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Free Radical Research",
title = "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems",
pages = "S72-S72",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_167"
}

Mojović, M., Spasojević, I., Spasić, M.,& Bačić, G.. (2006). Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research
Taylor & Francis Ltd, Abingdon., 40, S72-S72.
https://hdl.handle.net/21.15107/rcub_rimsi_167

Mojović M, Spasojević I, Spasić M, Bačić G. Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research. 2006;40:S72-S72.
https://hdl.handle.net/21.15107/rcub_rimsi_167 .

Mojović, Miloš, Spasojević, Ivan, Spasić, Mihajlo, Bačić, Goran, "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems" in Free Radical Research, 40 (2006):S72-S72,
https://hdl.handle.net/21.15107/rcub_rimsi_167 .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Z.
AU  - Batas, V.
AU  - Bačić, Goran
AU  - Spasić, Mihajlo
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/164
PB  - Taylor & Francis Ltd, Abingdon
T2  - Free Radical Research
T1  - Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS
EP  - S90
SP  - S90
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_164
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Z. and Batas, V. and Bačić, Goran and Spasić, Mihajlo",
year = "2006",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Free Radical Research",
title = "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS",
pages = "S90-S90",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_164"
}

Spasojević, I., Mojović, M., Stević, Z., Batas, V., Bačić, G.,& Spasić, M.. (2006). Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research
Taylor & Francis Ltd, Abingdon., 40, S90-S90.
https://hdl.handle.net/21.15107/rcub_rimsi_164

Spasojević I, Mojović M, Stević Z, Batas V, Bačić G, Spasić M. Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research. 2006;40:S90-S90.
https://hdl.handle.net/21.15107/rcub_rimsi_164 .

Spasojević, Ivan, Mojović, Miloš, Stević, Z., Batas, V., Bačić, Goran, Spasić, Mihajlo, "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS" in Free Radical Research, 40 (2006):S90-S90,
https://hdl.handle.net/21.15107/rcub_rimsi_164 .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Maksimović, Vuk
AU  - Zakrzewska, Joanna
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/140
AB  - In the present study, we showed that the antineoplastic drug 5-fluorouracil (5-FU) induces in vitro exposure-time/dose-dependent changes on the level of an erythrocyte's morphology, ionic balance, and membrane fluidity. These changes are partially or fully irreversible, and we suggest that they are provoked by an irreversible depletion of ATP. Because of these changes that could also occur in vivo during 5-FU infusion, a certain amount of erythrocytes with echinocytic shape and diminished ability to deliver oxygen is present in blood for longer periods of time. This renders oxygen transport and delivery more difficult, leaving the heart with an insufficient supply of oxygen, thus leading to cardiotoxicity.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Chemical Information and Modeling
T1  - Effects of 5-fluorouracil on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning
EP  - 1685
IS  - 6
SP  - 1680
VL  - 45
DO  - 10.1021/ci0501746
ER  - 

@article{
author = "Spasojević, Ivan and Maksimović, Vuk and Zakrzewska, Joanna and Bačić, Goran",
year = "2005",
abstract = "In the present study, we showed that the antineoplastic drug 5-fluorouracil (5-FU) induces in vitro exposure-time/dose-dependent changes on the level of an erythrocyte's morphology, ionic balance, and membrane fluidity. These changes are partially or fully irreversible, and we suggest that they are provoked by an irreversible depletion of ATP. Because of these changes that could also occur in vivo during 5-FU infusion, a certain amount of erythrocytes with echinocytic shape and diminished ability to deliver oxygen is present in blood for longer periods of time. This renders oxygen transport and delivery more difficult, leaving the heart with an insufficient supply of oxygen, thus leading to cardiotoxicity.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Chemical Information and Modeling",
title = "Effects of 5-fluorouracil on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning",
pages = "1685-1680",
number = "6",
volume = "45",
doi = "10.1021/ci0501746"
}

Spasojević, I., Maksimović, V., Zakrzewska, J.,& Bačić, G.. (2005). Effects of 5-fluorouracil on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning. in Journal of Chemical Information and Modeling
Amer Chemical Soc, Washington., 45(6), 1680-1685.
https://doi.org/10.1021/ci0501746

Spasojević I, Maksimović V, Zakrzewska J, Bačić G. Effects of 5-fluorouracil on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning. in Journal of Chemical Information and Modeling. 2005;45(6):1680-1685.
doi:10.1021/ci0501746 .

Spasojević, Ivan, Maksimović, Vuk, Zakrzewska, Joanna, Bačić, Goran, "Effects of 5-fluorouracil on erythrocytes in relation to its cardiotoxicity: Membrane structure and functioning" in Journal of Chemical Information and Modeling, 45, no. 6 (2005):1680-1685,
https://doi.org/10.1021/ci0501746 . .

TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/122
AB  - We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Chemical Information and Modeling
T1  - Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems
EP  - 1718
IS  - 6
SP  - 1716
VL  - 45
DO  - 10.1021/ci050173d
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Bačić, Goran",
year = "2005",
abstract = "We proposed EPR spectroscopy using spin-trap DEPMPO as a novel method for the detection of a hydrogen atom (H-center dot) produced by chemical and biological systems. In complex EPR spectra of DEPMPO adducts in biological systems, spectral lines of unknown origin have been observed. We have assumed (Bacic, G.; Mojovic, M. Ann. N. Y. Acod. Sci. 2005, 1048, 230-243) that those lines represent the spectrum of a hydrogen atom (H-center dot) adduct i.e., DEPMPO/H. Ail electrochemical system known to produce only H-center dot radicals was used here in order to obtain a separate spectrum of the DEPMPO/H adduct. An acquired spectrum as well as a computer spectral simulation of the DEPMPO/H adduct showed considerable resemblance with additional lines in the EPR spectra of DEPMPO adducts in biological systems-plant plasma membranes and cell walls. This shows that such a radical is produced by plants as well as that DEPMPO is suitable for detection in both electrochemical and biological systems.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Chemical Information and Modeling",
title = "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems",
pages = "1718-1716",
number = "6",
volume = "45",
doi = "10.1021/ci050173d"
}

Mojović, M., Spasojević, I.,& Bačić, G.. (2005). Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling
Amer Chemical Soc, Washington., 45(6), 1716-1718.
https://doi.org/10.1021/ci050173d

Mojović M, Spasojević I, Bačić G. Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems. in Journal of Chemical Information and Modeling. 2005;45(6):1716-1718.
doi:10.1021/ci050173d .

Mojović, Miloš, Spasojević, Ivan, Bačić, Goran, "Detection of hydrogen atom adduct of spin-trap DEPMPO. The relevance for studies of biological systems" in Journal of Chemical Information and Modeling, 45, no. 6 (2005):1716-1718,
https://doi.org/10.1021/ci050173d . .

TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Vuletic, M
AU  - Vučinić, Željko
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/142
AB  - Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
EP  - 186
IS  - 2
SP  - 177
VL  - 70
DO  - 10.2298/JSC0502177M
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Vuletic, M and Vučinić, Željko and Bačić, Goran",
year = "2005",
abstract = "Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes",
pages = "186-177",
number = "2",
volume = "70",
doi = "10.2298/JSC0502177M"
}

Mojović, M., Spasojević, I., Vuletic, M., Vučinić, Ž.,& Bačić, G.. (2005). An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(2), 177-186.
https://doi.org/10.2298/JSC0502177M

Mojović M, Spasojević I, Vuletic M, Vučinić Ž, Bačić G. An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society. 2005;70(2):177-186.
doi:10.2298/JSC0502177M .

Mojović, Miloš, Spasojević, Ivan, Vuletic, M, Vučinić, Željko, Bačić, Goran, "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes" in Journal of the Serbian Chemical Society, 70, no. 2 (2005):177-186,
https://doi.org/10.2298/JSC0502177M . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Zakrzewska, Joanna
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/124
AB  - Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism
EP  - 320
SP  - 311
VL  - 1048
DO  - 10.1196/annals.1342.028
ER  - 

@article{
author = "Spasojević, Ivan and Zakrzewska, Joanna and Bačić, Goran",
year = "2005",
abstract = "Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism",
pages = "320-311",
volume = "1048",
doi = "10.1196/annals.1342.028"
}

Spasojević, I., Zakrzewska, J.,& Bačić, G.. (2005). P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 311-320.
https://doi.org/10.1196/annals.1342.028

Spasojević I, Zakrzewska J, Bačić G. P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:311-320.
doi:10.1196/annals.1342.028 .

Spasojević, Ivan, Zakrzewska, Joanna, Bačić, Goran, "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):311-320,
https://doi.org/10.1196/annals.1342.028 . .