P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism
Апстракт
Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply... of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.
Кључне речи:
P-31 NMR spectroscopy / oxygen / erythrocytes / cardiotoxicity / 5-FUИзвор:
Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 2005, 1048, 311-320Издавач:
- New York Acad Sciences, New York
DOI: 10.1196/annals.1342.028
ISSN: 0077-8923
PubMed: 16154943
WoS: 000233712300027
Scopus: 2-s2.0-25144480370
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Spasojević, Ivan AU - Zakrzewska, Joanna AU - Bačić, Goran PY - 2005 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/124 AB - Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes. PB - New York Acad Sciences, New York T2 - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus T1 - P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism EP - 320 SP - 311 VL - 1048 DO - 10.1196/annals.1342.028 ER -
@article{ author = "Spasojević, Ivan and Zakrzewska, Joanna and Bačić, Goran", year = "2005", abstract = "Antineoplastic drug 5-fluorouracil (5-FU) frequently shows cardiotoxic effects, the mechanism of which has not yet been elucidated. The objective of the present study was to explore effects of 5-FU on metabolism of ATP, 2,3-BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5-FU cardiotoxicity. We determined that 5-FU induced rapid increase in 02 consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO(2) provoked increase in production of 2,3-BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5-FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non-functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.", publisher = "New York Acad Sciences, New York", journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus", title = "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism", pages = "320-311", volume = "1048", doi = "10.1196/annals.1342.028" }
Spasojević, I., Zakrzewska, J.,& Bačić, G.. (2005). P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus New York Acad Sciences, New York., 1048, 311-320. https://doi.org/10.1196/annals.1342.028
Spasojević I, Zakrzewska J, Bačić G. P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:311-320. doi:10.1196/annals.1342.028 .
Spasojević, Ivan, Zakrzewska, Joanna, Bačić, Goran, "P-31 NMR spectroscopy and polarographic combined study of erythrocytes treated with 5-fluorouracil - Cardiotoxicity-related changes in ATP, 2,3-BPG, and O-2 metabolism" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):311-320, https://doi.org/10.1196/annals.1342.028 . .