TY  - JOUR
AU  - Žižić, Milan
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Živić, Miroslav
AU  - Stanić, Marina
AU  - Maksimović, Vuk
AU  - Zakrzewska, Joanna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3165
AB  - BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Biology and Medicine
T1  - Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass
EP  - 127386
SP  - 127381
VL  - 83
DO  - 10.1016/j.jtemb.2024.127381
ER  - 

@article{
author = "Žižić, Milan and Atlagić, Kristina and Karaman, Maja and Živić, Miroslav and Stanić, Marina and Maksimović, Vuk and Zakrzewska, Joanna",
year = "2024",
abstract = "BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Biology and Medicine",
title = "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass",
pages = "127386-127381",
volume = "83",
doi = "10.1016/j.jtemb.2024.127381"
}

Žižić, M., Atlagić, K., Karaman, M., Živić, M., Stanić, M., Maksimović, V.,& Zakrzewska, J.. (2024). Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine
Elsevier Gmbh, Munich., 83, 127381-127386.
https://doi.org/10.1016/j.jtemb.2024.127381

Žižić M, Atlagić K, Karaman M, Živić M, Stanić M, Maksimović V, Zakrzewska J. Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine. 2024;83:127381-127386.
doi:10.1016/j.jtemb.2024.127381 .

Žižić, Milan, Atlagić, Kristina, Karaman, Maja, Živić, Miroslav, Stanić, Marina, Maksimović, Vuk, Zakrzewska, Joanna, "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass" in Journal of Trace Elements in Biology and Medicine, 83 (2024):127381-127386,
https://doi.org/10.1016/j.jtemb.2024.127381 . .

TY  - JOUR
AU  - Hafner, Aljoša
AU  - Costa, Luca
AU  - Kourousias, George
AU  - Bonanni, Valentina
AU  - Žižić, Milan
AU  - Stolfa, Andrea
AU  - Bazi, Benjamin
AU  - Vincze, Laszlo
AU  - Gianoncelli, Alessandra
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3179
AB  - Multimodal imaging and spectroscopy like concurrent Scanning
Transmission X-ray Microscopy (STXM) and X-ray Fluorescence
(XRF) are highly desirable as they allow retrieving complementary
information. This paper reports on the design, development,
integration and field testing of a novel in-situ Atomic Force
Microscopy (AFM) instrument for operation in high vacuum in a
synchrotron soft X-ray microscopy STXM-XRF end-station. A
combination of XRF-AFM is demonstrated for the first time in the
soft X-ray regime, with an outlook for the full XRF-STXM-AFM combination.
PB  - Royal Society of Chemistry
T2  - Analyst
T1  - An innovative in-situ AFM system for a soft X-ray spectromicroscopy synchrotron beamline
EP  - 706
IS  - 3
SP  - 700
VL  - 149
DO  - 10.1039/D3AN01358H
ER  - 

@article{
author = "Hafner, Aljoša and Costa, Luca and Kourousias, George and Bonanni, Valentina and Žižić, Milan and Stolfa, Andrea and Bazi, Benjamin and Vincze, Laszlo and Gianoncelli, Alessandra",
year = "2024",
abstract = "Multimodal imaging and spectroscopy like concurrent Scanning
Transmission X-ray Microscopy (STXM) and X-ray Fluorescence
(XRF) are highly desirable as they allow retrieving complementary
information. This paper reports on the design, development,
integration and field testing of a novel in-situ Atomic Force
Microscopy (AFM) instrument for operation in high vacuum in a
synchrotron soft X-ray microscopy STXM-XRF end-station. A
combination of XRF-AFM is demonstrated for the first time in the
soft X-ray regime, with an outlook for the full XRF-STXM-AFM combination.",
publisher = "Royal Society of Chemistry",
journal = "Analyst",
title = "An innovative in-situ AFM system for a soft X-ray spectromicroscopy synchrotron beamline",
pages = "706-700",
number = "3",
volume = "149",
doi = "10.1039/D3AN01358H"
}

Hafner, A., Costa, L., Kourousias, G., Bonanni, V., Žižić, M., Stolfa, A., Bazi, B., Vincze, L.,& Gianoncelli, A.. (2024). An innovative in-situ AFM system for a soft X-ray spectromicroscopy synchrotron beamline. in Analyst
Royal Society of Chemistry., 149(3), 700-706.
https://doi.org/10.1039/D3AN01358H

Hafner A, Costa L, Kourousias G, Bonanni V, Žižić M, Stolfa A, Bazi B, Vincze L, Gianoncelli A. An innovative in-situ AFM system for a soft X-ray spectromicroscopy synchrotron beamline. in Analyst. 2024;149(3):700-706.
doi:10.1039/D3AN01358H .

Hafner, Aljoša, Costa, Luca, Kourousias, George, Bonanni, Valentina, Žižić, Milan, Stolfa, Andrea, Bazi, Benjamin, Vincze, Laszlo, Gianoncelli, Alessandra, "An innovative in-situ AFM system for a soft X-ray spectromicroscopy synchrotron beamline" in Analyst, 149, no. 3 (2024):700-706,
https://doi.org/10.1039/D3AN01358H . .

TY  - JOUR
AU  - Antonijević, Marko
AU  - Avdović, Edina
AU  - Simijonović, Dušica
AU  - Milanović, Žiko
AU  - Žižić, Milan
AU  - Marković, Zoran
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3163
AB  - Pharmaceutical and industrial utilization of synthetic chemicals has an immerse impact
on the environment. In that sense, novel chemicals with potential for industrial
application should be investigated for their behavior in reactions with hydroxyl radical,
simulating AOPs (Advanced Oxidation Processes). AOPs are known for being highly
effective in wastewater management and natural water remediation. In this paper,
exhaustive research on the radical scavenging activity of a newly synthesized
coumarin derivative (4HCBH), as a representative of the series of coumarin-
benzohydrazides with high antioxidative potential was conducted. This study took into
consideration the pH value range significant for practically all living organisms (pH=7.0-
8.5). According to the experimentally obtained results, the 4HCBH showed an increase
in radical scavenging activity, following the slight increase in pH values, which
suggested that the formation of anionic form of 4HCBH is responsible for its antiradical
activity. Further investigations led to the postulation of a novel mechanistic approach
called Sequential Proton Loss Electron Transfer – Radical-Radical Coupling (SPLET-
RRC), in which, by a series of steps, a new, stable compound was formed.
Furthermore, it was demonstrated that the product generated through SPLET-RRC
showed lower toxicity than the parent molecule.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Investigation of Novel Radical Scavenging Mechanisms in the Alkaline Environment: Green, Sustainable and Environmentally Friendly Antioxidative Agent(s)
EP  - 160319
SP  - 169307
VL  - 912
DO  - 10.1016/j.scitotenv.2023.169307
ER  - 

@article{
author = "Antonijević, Marko and Avdović, Edina and Simijonović, Dušica and Milanović, Žiko and Žižić, Milan and Marković, Zoran",
year = "2024",
abstract = "Pharmaceutical and industrial utilization of synthetic chemicals has an immerse impact
on the environment. In that sense, novel chemicals with potential for industrial
application should be investigated for their behavior in reactions with hydroxyl radical,
simulating AOPs (Advanced Oxidation Processes). AOPs are known for being highly
effective in wastewater management and natural water remediation. In this paper,
exhaustive research on the radical scavenging activity of a newly synthesized
coumarin derivative (4HCBH), as a representative of the series of coumarin-
benzohydrazides with high antioxidative potential was conducted. This study took into
consideration the pH value range significant for practically all living organisms (pH=7.0-
8.5). According to the experimentally obtained results, the 4HCBH showed an increase
in radical scavenging activity, following the slight increase in pH values, which
suggested that the formation of anionic form of 4HCBH is responsible for its antiradical
activity. Further investigations led to the postulation of a novel mechanistic approach
called Sequential Proton Loss Electron Transfer – Radical-Radical Coupling (SPLET-
RRC), in which, by a series of steps, a new, stable compound was formed.
Furthermore, it was demonstrated that the product generated through SPLET-RRC
showed lower toxicity than the parent molecule.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Investigation of Novel Radical Scavenging Mechanisms in the Alkaline Environment: Green, Sustainable and Environmentally Friendly Antioxidative Agent(s)",
pages = "160319-169307",
volume = "912",
doi = "10.1016/j.scitotenv.2023.169307"
}

Antonijević, M., Avdović, E., Simijonović, D., Milanović, Ž., Žižić, M.,& Marković, Z.. (2024). Investigation of Novel Radical Scavenging Mechanisms in the Alkaline Environment: Green, Sustainable and Environmentally Friendly Antioxidative Agent(s). in Science of the Total Environment
Elsevier., 912, 169307-160319.
https://doi.org/10.1016/j.scitotenv.2023.169307

Antonijević M, Avdović E, Simijonović D, Milanović Ž, Žižić M, Marković Z. Investigation of Novel Radical Scavenging Mechanisms in the Alkaline Environment: Green, Sustainable and Environmentally Friendly Antioxidative Agent(s). in Science of the Total Environment. 2024;912:169307-160319.
doi:10.1016/j.scitotenv.2023.169307 .

Antonijević, Marko, Avdović, Edina, Simijonović, Dušica, Milanović, Žiko, Žižić, Milan, Marković, Zoran, "Investigation of Novel Radical Scavenging Mechanisms in the Alkaline Environment: Green, Sustainable and Environmentally Friendly Antioxidative Agent(s)" in Science of the Total Environment, 912 (2024):169307-160319,
https://doi.org/10.1016/j.scitotenv.2023.169307 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Santrač, Isidora
AU  - Danilović Luković, Jelena
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Zechmann, Bernd
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3144
AB  - Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a
remarkable metabolic and structural adaptability that allows it to thrive in metal-infested
environments. H. pluvialis could be potentially used in the remediation of waters polluted
with metals, such as manganese (Mn). Mn is also an essential element involved in different
metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we
examined morphological response of metabolically active green cell type of H. pluvialis
(microzooids) to high Mn concentrations exceeding their physiological quota. When exposed
to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma
atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after
1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24
h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1
hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs
showed that some cells had wall rupture and degraded intracellular content and damaged
organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by
thickened cell wall, preserved intracellular compartments, and reduced total area of lipid
droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of
Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced
cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall
thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations
exemplified by cell wall thickening, reduction in lipid droplets total area due to increased
energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural
adaptability. Further investigation using analytical methods will provide a more profound
understanding of the metabolic dimensions of adaptive response
PB  - UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade
C3  - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
T1  - Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess
EP  - 44
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3144
ER  - 

@conference{
author = "Santrač, Isidora and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Zechmann, Bernd and Žižić, Milan and Spasojević, Ivan",
year = "2023",
abstract = "Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a
remarkable metabolic and structural adaptability that allows it to thrive in metal-infested
environments. H. pluvialis could be potentially used in the remediation of waters polluted
with metals, such as manganese (Mn). Mn is also an essential element involved in different
metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we
examined morphological response of metabolically active green cell type of H. pluvialis
(microzooids) to high Mn concentrations exceeding their physiological quota. When exposed
to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma
atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after
1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24
h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1
hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs
showed that some cells had wall rupture and degraded intracellular content and damaged
organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by
thickened cell wall, preserved intracellular compartments, and reduced total area of lipid
droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of
Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced
cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall
thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations
exemplified by cell wall thickening, reduction in lipid droplets total area due to increased
energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural
adaptability. Further investigation using analytical methods will provide a more profound
understanding of the metabolic dimensions of adaptive response",
publisher = "UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade",
journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia",
title = "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3144"
}

Santrač, I., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S., Zechmann, B., Žižić, M.,& Spasojević, I.. (2023). Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade., 44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3144

Santrač I, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Zechmann B, Žižić M, Spasojević I. Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3144 .

Santrač, Isidora, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Zechmann, Bernd, Žižić, Milan, Spasojević, Ivan, "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):44-44,
https://hdl.handle.net/21.15107/rcub_rimsi_3144 .

TY  - JOUR
AU  - Milanović, Žiko
AU  - Dimic, Dusan
AU  - Antonijević, Marko
AU  - Žižić, Milan
AU  - Milenkovic, Dejan
AU  - Avdović, Edina
AU  - Marković, Zoran
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3164
AB  - The decomposition and chemical manipulation of stable aromatic pollutants into less toxic products is an
important topic for wastewater management and natural water remediation. The mechanism of the Advanced
Oxidation Process (AOPs) of 4,7-dihydroxycoumarin (4,7-DHC) and 7-hydroxycoumarin (7-HC), as examples of
stable naturally-occurring industrially-important compounds, in the presence of hydroxyl radical (HO•
) in the
aqueous solution has been analyzed using Electron Paramagnetic Resonance spectroscopy (EPR) and Quantum
Mechanics-based test for Overall Free Radical Scavenging Activity (QM-ORSA). The effect of pH values of the me-
dium on the investigated reaction mechanisms has been fully investigated. The rate constants were estimated by
the conventional transition state theory (TST) and Eckart’s method (ZCT_0). Estimated values of the overall rate
constant (koverall) higher than >4.06 × 109 M 1 s 1 at all pH values showed that both compounds undergo a
chemical transformation when exposed to HO•
. When pH increased in the range of 0–14, the koverall increased
from 4.06 × 109 to 1.11 × 1010 (4.7-DHC) and 2.09 × 109 to 1.76 × 1010 M 1s 1 (7-HC). At physiological pH =
7.4 value, 7-HC was ~1.5 times more prone to radical action, as shown by EPR and QM-ORSA, due to the
dominant anionic form. Both compounds were more reactive towards HO•
than Trolox at this pH value. The
ecotoxicity assessment of the starting compounds, intermediates and oxidation products indicated that the
formed products show lower acute and chronic toxicity effects on aquatic organisms than starting compounds,
which is a prerequisite for the development of novel AOPs procedures.
PB  - Elsevier
T2  - Chemical Engineering Journal
T1  - Influence of acid-base equilibria on the rate of the chemical reaction in the advanced oxidation processes: Coumarin derivatives and hydroxyl radical
EP  - 139661
SP  - 139648
VL  - 453
DO  - 10.1016/j.cej.2022.139648
ER  - 

@article{
author = "Milanović, Žiko and Dimic, Dusan and Antonijević, Marko and Žižić, Milan and Milenkovic, Dejan and Avdović, Edina and Marković, Zoran",
year = "2023",
abstract = "The decomposition and chemical manipulation of stable aromatic pollutants into less toxic products is an
important topic for wastewater management and natural water remediation. The mechanism of the Advanced
Oxidation Process (AOPs) of 4,7-dihydroxycoumarin (4,7-DHC) and 7-hydroxycoumarin (7-HC), as examples of
stable naturally-occurring industrially-important compounds, in the presence of hydroxyl radical (HO•
) in the
aqueous solution has been analyzed using Electron Paramagnetic Resonance spectroscopy (EPR) and Quantum
Mechanics-based test for Overall Free Radical Scavenging Activity (QM-ORSA). The effect of pH values of the me-
dium on the investigated reaction mechanisms has been fully investigated. The rate constants were estimated by
the conventional transition state theory (TST) and Eckart’s method (ZCT_0). Estimated values of the overall rate
constant (koverall) higher than >4.06 × 109 M 1 s 1 at all pH values showed that both compounds undergo a
chemical transformation when exposed to HO•
. When pH increased in the range of 0–14, the koverall increased
from 4.06 × 109 to 1.11 × 1010 (4.7-DHC) and 2.09 × 109 to 1.76 × 1010 M 1s 1 (7-HC). At physiological pH =
7.4 value, 7-HC was ~1.5 times more prone to radical action, as shown by EPR and QM-ORSA, due to the
dominant anionic form. Both compounds were more reactive towards HO•
than Trolox at this pH value. The
ecotoxicity assessment of the starting compounds, intermediates and oxidation products indicated that the
formed products show lower acute and chronic toxicity effects on aquatic organisms than starting compounds,
which is a prerequisite for the development of novel AOPs procedures.",
publisher = "Elsevier",
journal = "Chemical Engineering Journal",
title = "Influence of acid-base equilibria on the rate of the chemical reaction in the advanced oxidation processes: Coumarin derivatives and hydroxyl radical",
pages = "139661-139648",
volume = "453",
doi = "10.1016/j.cej.2022.139648"
}

Milanović, Ž., Dimic, D., Antonijević, M., Žižić, M., Milenkovic, D., Avdović, E.,& Marković, Z.. (2023). Influence of acid-base equilibria on the rate of the chemical reaction in the advanced oxidation processes: Coumarin derivatives and hydroxyl radical. in Chemical Engineering Journal
Elsevier., 453, 139648-139661.
https://doi.org/10.1016/j.cej.2022.139648

Milanović Ž, Dimic D, Antonijević M, Žižić M, Milenkovic D, Avdović E, Marković Z. Influence of acid-base equilibria on the rate of the chemical reaction in the advanced oxidation processes: Coumarin derivatives and hydroxyl radical. in Chemical Engineering Journal. 2023;453:139648-139661.
doi:10.1016/j.cej.2022.139648 .

Milanović, Žiko, Dimic, Dusan, Antonijević, Marko, Žižić, Milan, Milenkovic, Dejan, Avdović, Edina, Marković, Zoran, "Influence of acid-base equilibria on the rate of the chemical reaction in the advanced oxidation processes: Coumarin derivatives and hydroxyl radical" in Chemical Engineering Journal, 453 (2023):139648-139661,
https://doi.org/10.1016/j.cej.2022.139648 . .

TY  - CONF
AU  - Santrač, Isidora
AU  - Žižić, Milan
AU  - AQUILANTI, Giuliana
AU  - Gianoncelli, Alessandra
AU  - Bonanni, Valentina
AU  - Danilović Luković, Jelena
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3139
AB  - Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.
C3  - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
T1  - The coordination and storage of manganese in the microalga Haematococcus pluvialis
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3139
ER  - 

@conference{
author = "Santrač, Isidora and Žižić, Milan and AQUILANTI, Giuliana and Gianoncelli, Alessandra and Bonanni, Valentina and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Spasojević, Ivan",
year = "2023",
abstract = "Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.",
journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia",
title = "The coordination and storage of manganese in the microalga Haematococcus pluvialis",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3139"
}

Santrač, I., Žižić, M., AQUILANTI, G., Gianoncelli, A., Bonanni, V., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S.,& Spasojević, I.. (2023). The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia, 45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139

Santrač I, Žižić M, AQUILANTI G, Gianoncelli A, Bonanni V, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Spasojević I. The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

Santrač, Isidora, Žižić, Milan, AQUILANTI, Giuliana, Gianoncelli, Alessandra, Bonanni, Valentina, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Spasojević, Ivan, "The coordination and storage of manganese in the microalga Haematococcus pluvialis" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):45,
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

TY  - JOUR
AU  - Milanović, Žiko
AU  - Dimić, Dušan
AU  - Klein, Erik
AU  - Biela, Monika
AU  - Lukeš, Vladimir
AU  - Žižić, Milan
AU  - Avdović, Edina
AU  - Bešlo, Drago
AU  - Vojinović, Radiša
AU  - Dimitrić-Marković, Jasmina
AU  - Marković, Zoran
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2111
AB  - Coumarins represent a broad class of compounds with pronounced pharmacological properties and therapeutic potential. The pursuit of the commercialization of these compounds requires the establishment of controlled and highly efficient degradation processes, such as advanced oxidation processes (AOPs). Application of this methodology necessitates a comprehensive understanding of the degradation mechanisms of these compounds. For this reason, possible reaction routes between HO• and recently synthesized aminophenol 4,7-dihydroxycoumarin derivatives, as model systems, were examined using electron paramagnetic resonance (EPR) spectroscopy and a quantum mechanical approach (a QM-ORSA methodology) based on density functional theory (DFT). The EPR results indicated that all compounds had significantly reduced amounts of HO• radicals present in the reaction system under physiological conditions. The kinetic DFT study showed that all investigated compounds reacted with HO• via HAT/PCET and SPLET mechanisms. The estimated overall rate constants (koverall) correlated with the EPR results satisfactorily. Unlike HO• radicals, the newly formed radicals did not show (or showed negligible) activity towards biomolecule models representing biological targets. Inactivation of the formed radical species through the synergistic action of O2/NOx or the subsequent reaction with HO• was thermodynamically favored. The ecotoxicity assessment of the starting compounds and oxidation products, formed in multistage reactions with O2/NOx and HO•, indicated that the formed products showed lower acute and chronic toxicity effects on aquatic organisms than the starting compounds, which is a prerequisite for the application of AOPs procedures in the degradation of compounds.
PB  - MDPI, Basel, Switzerland
T2  - International Jornal of Environmental Research and Public Health
T1  - Derivatives in Advanced Oxidation Processes: Experimental and Kinetic DFT Stud
EP  - 2064
SP  - 2046
VL  - 20
DO  - 10.3390/ijerph20032046
ER  - 

@article{
author = "Milanović, Žiko and Dimić, Dušan and Klein, Erik and Biela, Monika and Lukeš, Vladimir and Žižić, Milan and Avdović, Edina and Bešlo, Drago and Vojinović, Radiša and Dimitrić-Marković, Jasmina and Marković, Zoran",
year = "2023",
abstract = "Coumarins represent a broad class of compounds with pronounced pharmacological properties and therapeutic potential. The pursuit of the commercialization of these compounds requires the establishment of controlled and highly efficient degradation processes, such as advanced oxidation processes (AOPs). Application of this methodology necessitates a comprehensive understanding of the degradation mechanisms of these compounds. For this reason, possible reaction routes between HO• and recently synthesized aminophenol 4,7-dihydroxycoumarin derivatives, as model systems, were examined using electron paramagnetic resonance (EPR) spectroscopy and a quantum mechanical approach (a QM-ORSA methodology) based on density functional theory (DFT). The EPR results indicated that all compounds had significantly reduced amounts of HO• radicals present in the reaction system under physiological conditions. The kinetic DFT study showed that all investigated compounds reacted with HO• via HAT/PCET and SPLET mechanisms. The estimated overall rate constants (koverall) correlated with the EPR results satisfactorily. Unlike HO• radicals, the newly formed radicals did not show (or showed negligible) activity towards biomolecule models representing biological targets. Inactivation of the formed radical species through the synergistic action of O2/NOx or the subsequent reaction with HO• was thermodynamically favored. The ecotoxicity assessment of the starting compounds and oxidation products, formed in multistage reactions with O2/NOx and HO•, indicated that the formed products showed lower acute and chronic toxicity effects on aquatic organisms than the starting compounds, which is a prerequisite for the application of AOPs procedures in the degradation of compounds.",
publisher = "MDPI, Basel, Switzerland",
journal = "International Jornal of Environmental Research and Public Health",
title = "Derivatives in Advanced Oxidation Processes: Experimental and Kinetic DFT Stud",
pages = "2064-2046",
volume = "20",
doi = "10.3390/ijerph20032046"
}

Milanović, Ž., Dimić, D., Klein, E., Biela, M., Lukeš, V., Žižić, M., Avdović, E., Bešlo, D., Vojinović, R., Dimitrić-Marković, J.,& Marković, Z.. (2023). Derivatives in Advanced Oxidation Processes: Experimental and Kinetic DFT Stud. in International Jornal of Environmental Research and Public Health
MDPI, Basel, Switzerland., 20, 2046-2064.
https://doi.org/10.3390/ijerph20032046

Milanović Ž, Dimić D, Klein E, Biela M, Lukeš V, Žižić M, Avdović E, Bešlo D, Vojinović R, Dimitrić-Marković J, Marković Z. Derivatives in Advanced Oxidation Processes: Experimental and Kinetic DFT Stud. in International Jornal of Environmental Research and Public Health. 2023;20:2046-2064.
doi:10.3390/ijerph20032046 .

Milanović, Žiko, Dimić, Dušan, Klein, Erik, Biela, Monika, Lukeš, Vladimir, Žižić, Milan, Avdović, Edina, Bešlo, Drago, Vojinović, Radiša, Dimitrić-Marković, Jasmina, Marković, Zoran, "Derivatives in Advanced Oxidation Processes: Experimental and Kinetic DFT Stud" in International Jornal of Environmental Research and Public Health, 20 (2023):2046-2064,
https://doi.org/10.3390/ijerph20032046 . .

TY  - JOUR
AU  - Gianoncelli, Alessandra
AU  - Žižić, Milan
AU  - Bonanni, Valentina
AU  - Birarda, Giovanni
AU  - Bedolla Orozco, Diana Eva
AU  - Vogel-Mikus, Katarina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2109
AB  - .The radiation damage issue consequent to soft X-rays’ exposure is still an important aspect to be contemplated in soft X-ray microscopy. The work presented here is part of a more
extended investigation on the topic and targets the effects of soft X-rays exposure on plant tissues.
The aftermaths of soft X-rays’ exposure were evaluated by FTIR spectromicroscopy by comparing irradiated and non-irradiated areas on similar plant structural features, one day and ten days after irradiation, to highlight also a possible time-dependent behavior consequent to air exposure.
Our results show partial degradation of lignocellulosic complex and oxidation of cellulose and most probably of hemicellulose; lignin moiety of cell walls in vascular tissue however remains stable.
Comparing the current study with our previous works it appears that, as expected, plant tissues seem less prone to soft X-rays radiation damage compared to mammalian cells or tissues.
PB  - Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK
T2  - Journal of Electron Spectroscopy and Related Phenomena
T1  - Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy
EP  - 147391
SP  - 147384
VL  - 267
DO  - 10.1016/j.elspec.2023.147384
ER  - 

@article{
author = "Gianoncelli, Alessandra and Žižić, Milan and Bonanni, Valentina and Birarda, Giovanni and Bedolla Orozco, Diana Eva and Vogel-Mikus, Katarina",
year = "2023",
abstract = ".The radiation damage issue consequent to soft X-rays’ exposure is still an important aspect to be contemplated in soft X-ray microscopy. The work presented here is part of a more
extended investigation on the topic and targets the effects of soft X-rays exposure on plant tissues.
The aftermaths of soft X-rays’ exposure were evaluated by FTIR spectromicroscopy by comparing irradiated and non-irradiated areas on similar plant structural features, one day and ten days after irradiation, to highlight also a possible time-dependent behavior consequent to air exposure.
Our results show partial degradation of lignocellulosic complex and oxidation of cellulose and most probably of hemicellulose; lignin moiety of cell walls in vascular tissue however remains stable.
Comparing the current study with our previous works it appears that, as expected, plant tissues seem less prone to soft X-rays radiation damage compared to mammalian cells or tissues.",
publisher = "Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK",
journal = "Journal of Electron Spectroscopy and Related Phenomena",
title = "Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy",
pages = "147391-147384",
volume = "267",
doi = "10.1016/j.elspec.2023.147384"
}

Gianoncelli, A., Žižić, M., Bonanni, V., Birarda, G., Bedolla Orozco, D. E.,& Vogel-Mikus, K.. (2023). Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy. in Journal of Electron Spectroscopy and Related Phenomena
Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK., 267, 147384-147391.
https://doi.org/10.1016/j.elspec.2023.147384

Gianoncelli A, Žižić M, Bonanni V, Birarda G, Bedolla Orozco DE, Vogel-Mikus K. Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy. in Journal of Electron Spectroscopy and Related Phenomena. 2023;267:147384-147391.
doi:10.1016/j.elspec.2023.147384 .

Gianoncelli, Alessandra, Žižić, Milan, Bonanni, Valentina, Birarda, Giovanni, Bedolla Orozco, Diana Eva, Vogel-Mikus, Katarina, "Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy" in Journal of Electron Spectroscopy and Related Phenomena, 267 (2023):147384-147391,
https://doi.org/10.1016/j.elspec.2023.147384 . .

TY  - CONF
AU  - Ćukić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Kovačević, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2419
AB  - Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana
EP  - 230
SP  - 230
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2419
ER  - 

@conference{
author = "Ćukić, Aleksandra and Dimitrijević, Milena and Kovačević, Snežana and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2022",
abstract = "Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana",
pages = "230-230",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2419"
}

Ćukić, A., Dimitrijević, M., Kovačević, S., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2022). Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419

Ćukić A, Dimitrijević M, Kovačević S, Opačić M, Stanić M, Žižić M, Spasojević I. Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

Ćukić, Aleksandra, Dimitrijević, Milena, Kovačević, Snežana, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):230-230,
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

TY  - CONF
AU  - Rodić, Ivanka
AU  - Lukičić, Jovana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2409
AB  - Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite
EP  - 233
SP  - 233
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2409
ER  - 

@conference{
author = "Rodić, Ivanka and Lukičić, Jovana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana",
year = "2022",
abstract = "Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite",
pages = "233-233",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2409"
}

Rodić, I., Lukičić, J., Stanić, M., Žižić, M., Zakrzewska, J., Živić, M.,& Cvetic Antic, T.. (2022). Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409

Rodić I, Lukičić J, Stanić M, Žižić M, Zakrzewska J, Živić M, Cvetic Antic T. Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

Rodić, Ivanka, Lukičić, Jovana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):233-233,
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2385
AB  - Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus
EP  - 858
SP  - 858
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2385
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2022",
abstract = "Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus",
pages = "858-858",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2385"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2022). Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):858-858,
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Cvetic Antic, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2113
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 96
SP  - 93
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2113
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Cvetic Antic, Tijana and Todorović, Nataša and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "96-93",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2113"
}

Žižić, M., Stanić, M., AQUILANTI, G., Cvetic Antic, T., Todorović, N., Živić, M.,& Zakrzewska, J.. (2022). SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113

Žižić M, Stanić M, AQUILANTI G, Cvetic Antic T, Todorović N, Živić M, Zakrzewska J. SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Cvetic Antic, Tijana, Todorović, Nataša, Živić, Miroslav, Zakrzewska, Joanna, "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):93-96,
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Rodić, Ivanka
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Todorović, Nataša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2114
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 92
SP  - 89
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2114
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and Rodić, Ivanka and Cvetic Antic, Tijana and Živić, Miroslav and Zakrzewska, Joanna and Todorović, Nataša",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "92-89",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2114"
}

Žižić, M., Stanić, M., Rodić, I., Cvetic Antic, T., Živić, M., Zakrzewska, J.,& Todorović, N.. (2022). PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114

Žižić M, Stanić M, Rodić I, Cvetic Antic T, Živić M, Zakrzewska J, Todorović N. PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

Žižić, Milan, Stanić, Marina, Rodić, Ivanka, Cvetic Antic, Tijana, Živić, Miroslav, Zakrzewska, Joanna, Todorović, Nataša, "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):89-92,
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

TY  - JOUR
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Bajuk-Bogdanovic, Danica
AU  - Branković, Goran
AU  - Rodić, Ivanka
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106
AB  - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
PB  - Springer-Verlag GmbH Berlin Heidelberg
T2  - Analytical and Bianalytical Chemistry
T1  - Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus
EP  - 6222.
IS  - 20
SP  - 6213
VL  - 414
DO  - 10.1007/s00216-022-04191-4
ER  - 

@article{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy",
publisher = "Springer-Verlag GmbH Berlin Heidelberg",
journal = "Analytical and Bianalytical Chemistry",
title = "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus",
pages = "6222.-6213",
number = "20",
volume = "414",
doi = "10.1007/s00216-022-04191-4"
}

Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry
Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222..
https://doi.org/10.1007/s00216-022-04191-4

Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222..
doi:10.1007/s00216-022-04191-4 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222.,
https://doi.org/10.1007/s00216-022-04191-4 . .

TY  - CONF
AU  - Lukicic, J.
AU  - Rodic, I.
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1463
AB  - Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.
PB  - Wiley, Hoboken
C3  - Febs Open Bio
T1  - Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus
EP  - 397
SP  - 396
VL  - 11
DO  - 10.1002/2211-5463.13205
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1463
ER  - 

@conference{
author = "Lukicic, J. and Rodic, I. and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.",
publisher = "Wiley, Hoboken",
journal = "Febs Open Bio",
title = "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus",
pages = "397-396",
volume = "11",
doi = "10.1002/2211-5463.13205",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1463"
}

Lukicic, J., Rodic, I., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio
Wiley, Hoboken., 11, 396-397.
https://doi.org/10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463

Lukicic J, Rodic I, Žižić M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio. 2021;11:396-397.
doi:10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

Lukicic, J., Rodic, I., Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus" in Febs Open Bio, 11 (2021):396-397,
https://doi.org/10.1002/2211-5463.13205 .,
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Dučić, Tanja
AU  - Stanković, Dalibor
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2391
AB  - Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)
EP  - 174
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2391
ER  - 

@conference{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Dučić, Tanja and Stanković, Dalibor and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2391"
}

Vojvodić, S., Dimitrijević, M., Dučić, T., Stanković, D., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2021). Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391

Vojvodić S, Dimitrijević M, Dučić T, Stanković D, Opačić M, Stanić M, Žižić M, Spasojević I. Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

Vojvodić, Snežana, Dimitrijević, Milena, Dučić, Tanja, Stanković, Dalibor, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):174-174,
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

TY  - CONF
AU  - Žižić, Milan
AU  - Živić, Miroslav
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Zakrzewska, Joanna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2203
AB  - Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great
potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus
truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The
submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR
spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of
vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome
vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR
measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite
containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced
through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexosephosphates.
Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the
fungal cell was confirmed.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021
C3  - 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.
T1  - INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM
EP  - 307
SP  - 304
DO  - 10.46793/ICCBI21.304Z
ER  - 

@conference{
author = "Žižić, Milan and Živić, Miroslav and Atlagić, Kristina and Karaman, Maja and Zakrzewska, Joanna",
year = "2021",
abstract = "Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great
potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus
truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The
submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR
spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of
vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome
vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR
measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite
containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced
through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexosephosphates.
Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the
fungal cell was confirmed.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021",
journal = "1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.",
title = "INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM",
pages = "307-304",
doi = "10.46793/ICCBI21.304Z"
}

Žižić, M., Živić, M., Atlagić, K., Karaman, M.,& Zakrzewska, J.. (2021). INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM. in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021., 304-307.
https://doi.org/10.46793/ICCBI21.304Z

Žižić M, Živić M, Atlagić K, Karaman M, Zakrzewska J. INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM. in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.. 2021;:304-307.
doi:10.46793/ICCBI21.304Z .

Žižić, Milan, Živić, Miroslav, Atlagić, Kristina, Karaman, Maja, Zakrzewska, Joanna, "INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM" in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia. (2021):304-307,
https://doi.org/10.46793/ICCBI21.304Z . .

TY  - JOUR
AU  - Milanović, Ziko
AU  - Dimic, Dusan
AU  - Žižić, Milan
AU  - Milenković, Dejan
AU  - Marković, Zoran
AU  - Avdović, Edina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1504
AB  - Coumarin derivatives have proven beneficial biological activities, but the mechanism of their radical scavenging potency is not fully understood. In this study, the antiradical capacity of two newly synthesized 4,7-dihydroxycoumarin derivatives: (E)-3-(1-((3-hydroxy-4-methoxyphenyl)amino)-ethylidene)-2,4-dioxochroman-7-yl acetate (A-3OH) and (E)-3-(1-((4-hydroxy-3-methoxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A-4OH) towards HO center dot were examined by Electron Paramagnetic Resonance (EPR) Spectroscopy and Density Functional Theory (DFT). The compounds were fully characterized by the elemental microanalysis, IR, and NMR spectroscopies. The effect of pH on the acid-base equilibria is separately discussed and the predominant species at the physiological pH were determined. Several common mechanisms (Hydrogen Atom Transfer (HAT), Single-Electron Transfer followed by Proton Transfer (SET-PT), Sequential Proton Loss followed by Electron Transfer (SPLET), Radical Adduct Formation (RAF), and Intramolecular Hydrogen Atom Abstraction (iHAA)) of radical scavenging were investigated based on thermodynamic and kinetic parameters. EPR results indicated that both compounds significantly reduce the amount of present HO center dot. The results of the kinetic DFT study demonstrated that both compounds predominantly exhibit antiradical capacity through HAT and SPLET mechanisms. The estimated overall rate constants (k(overall)) proved that A-4OH shows better antioxidant capacity than A-3OH which is well-correlated with the results obtained by EPR measurement.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study
IS  - 24
VL  - 22
DO  - 10.3390/ijms222413273
ER  - 

@article{
author = "Milanović, Ziko and Dimic, Dusan and Žižić, Milan and Milenković, Dejan and Marković, Zoran and Avdović, Edina",
year = "2021",
abstract = "Coumarin derivatives have proven beneficial biological activities, but the mechanism of their radical scavenging potency is not fully understood. In this study, the antiradical capacity of two newly synthesized 4,7-dihydroxycoumarin derivatives: (E)-3-(1-((3-hydroxy-4-methoxyphenyl)amino)-ethylidene)-2,4-dioxochroman-7-yl acetate (A-3OH) and (E)-3-(1-((4-hydroxy-3-methoxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate (A-4OH) towards HO center dot were examined by Electron Paramagnetic Resonance (EPR) Spectroscopy and Density Functional Theory (DFT). The compounds were fully characterized by the elemental microanalysis, IR, and NMR spectroscopies. The effect of pH on the acid-base equilibria is separately discussed and the predominant species at the physiological pH were determined. Several common mechanisms (Hydrogen Atom Transfer (HAT), Single-Electron Transfer followed by Proton Transfer (SET-PT), Sequential Proton Loss followed by Electron Transfer (SPLET), Radical Adduct Formation (RAF), and Intramolecular Hydrogen Atom Abstraction (iHAA)) of radical scavenging were investigated based on thermodynamic and kinetic parameters. EPR results indicated that both compounds significantly reduce the amount of present HO center dot. The results of the kinetic DFT study demonstrated that both compounds predominantly exhibit antiradical capacity through HAT and SPLET mechanisms. The estimated overall rate constants (k(overall)) proved that A-4OH shows better antioxidant capacity than A-3OH which is well-correlated with the results obtained by EPR measurement.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study",
number = "24",
volume = "22",
doi = "10.3390/ijms222413273"
}

Milanović, Z., Dimic, D., Žižić, M., Milenković, D., Marković, Z.,& Avdović, E.. (2021). Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study. in International Journal of Molecular Sciences
MDPI, Basel., 22(24).
https://doi.org/10.3390/ijms222413273

Milanović Z, Dimic D, Žižić M, Milenković D, Marković Z, Avdović E. Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study. in International Journal of Molecular Sciences. 2021;22(24).
doi:10.3390/ijms222413273 .

Milanović, Ziko, Dimic, Dusan, Žižić, Milan, Milenković, Dejan, Marković, Zoran, Avdović, Edina, "Mechanism of Antiradical Activity of Newly Synthesized 4,7-Dihydroxycoumarin Derivatives-Experimental and Kinetic DFT Study" in International Journal of Molecular Sciences, 22, no. 24 (2021),
https://doi.org/10.3390/ijms222413273 . .

TY  - CONF
AU  - Žižić, Milan
AU  - Macura, Melanija
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1470
AB  - Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.204
ER  - 

@conference{
author = "Žižić, Milan and Macura, Melanija and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.204"
}

Žižić, M., Macura, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://doi.org/10.1016/j.freeradbiomed.2021.08.204

Žižić M, Macura M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine. 2021;177.
doi:10.1016/j.freeradbiomed.2021.08.204 .

Žižić, Milan, Macura, Melanija, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR" in Free Radical Biology and Medicine, 177 (2021),
https://doi.org/10.1016/j.freeradbiomed.2021.08.204 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić-Marković, Jasmina
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav
AU  - Cvetic, Antic, Tijana N.
AU  - Žižić, Milan
AU  - Todorović, Nataša
AU  - Vukic, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1457
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić-Marković, Jasmina and Filipović, Nenad D. and Zivić, Miroslav and Cvetic, Antic, Tijana N. and Žižić, Milan and Todorović, Nataša and Vukic, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić-Marković, J., Filipović, N. D., Zivić, M., Cvetic, A. T. N., Žižić, M., Todorović, N., Vukic, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić-Marković J, Filipović ND, Zivić M, Cvetic ATN, Žižić M, Todorović N, Vukic M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić-Marković, Jasmina, Filipović, Nenad D., Zivić, Miroslav, Cvetic, Antic, Tijana N., Žižić, Milan, Todorović, Nataša, Vukic, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Bogdanović Pristov, Jelena
AU  - Žižić, Milan
AU  - Stanković, Dalibor
AU  - Bajuk-Bogdanović, Danica
AU  - Stanić, Marina
AU  - Hagen, Wilfred
AU  - Piccioli, Mario
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3187
AB  - The structure of biliverdin (BV) and its complexion capacity with Cu2+ in dependence of
solvent choice has been examined. We first examined coordinate/redox interactions of BV
with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman
spectroscopy, 1HNMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable
coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more
planar and energetically stable in the complex. The complex showed strong paramagnetic
effects that were attributed to an unpaired delocalized e−. The delocalized electron may come
from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+
or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of
both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing
agents. The biological effects of the stable BV metallocomplex containing a delocalized
unpaired electron should be further examined, and may provide an answer to the longstanding
question of high energy investment in the catabolism of BV, which represents a
relatively harmless molecule per se. The complex possibility was quite different after
changing the solvent. BV structure in DMSO was analyzed by using NMR techniques and
unrestricted density function theory simulations to explain the incapacity of BV to build
coordination complex(es) with Cu2+ in dimethyl sulfoxide, which was confirmed by UV-Vis,
EPR and NMR spectroscopy. NMR showed that N atoms of BV are protonated in all four
pyrrole rings. The structure is stabilized by two hydrogen bonds between NH moieties and
carbonyl oxygens from opposite terminal pyrrole rings, and by the bending of propionyl chain
with carboxyl group out of the plain toward central position of BV. The simulations of
deprotonated BV, which builds copper complexes in water and chloroform as described
previously, showed a different conformation and organization of hydrogen bonds. Taking into
account that deprotonation represents a critical step in coordinate bonds formation, the
protonation of an additional N atom may represent a key difference between the interactions
of BV with copper in different solvents.
PB  - COST Action CA15133
C3  - Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
T1  - Structure of biliverdin and its interaction with copper
EP  - 44
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3187
ER  - 

@conference{
author = "Dimitrijević, Milena and Bogdanović Pristov, Jelena and Žižić, Milan and Stanković, Dalibor and Bajuk-Bogdanović, Danica and Stanić, Marina and Hagen, Wilfred and Piccioli, Mario and Spasojević, Ivan",
year = "2019",
abstract = "The structure of biliverdin (BV) and its complexion capacity with Cu2+ in dependence of
solvent choice has been examined. We first examined coordinate/redox interactions of BV
with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman
spectroscopy, 1HNMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable
coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more
planar and energetically stable in the complex. The complex showed strong paramagnetic
effects that were attributed to an unpaired delocalized e−. The delocalized electron may come
from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+
or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of
both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing
agents. The biological effects of the stable BV metallocomplex containing a delocalized
unpaired electron should be further examined, and may provide an answer to the longstanding
question of high energy investment in the catabolism of BV, which represents a
relatively harmless molecule per se. The complex possibility was quite different after
changing the solvent. BV structure in DMSO was analyzed by using NMR techniques and
unrestricted density function theory simulations to explain the incapacity of BV to build
coordination complex(es) with Cu2+ in dimethyl sulfoxide, which was confirmed by UV-Vis,
EPR and NMR spectroscopy. NMR showed that N atoms of BV are protonated in all four
pyrrole rings. The structure is stabilized by two hydrogen bonds between NH moieties and
carbonyl oxygens from opposite terminal pyrrole rings, and by the bending of propionyl chain
with carboxyl group out of the plain toward central position of BV. The simulations of
deprotonated BV, which builds copper complexes in water and chloroform as described
previously, showed a different conformation and organization of hydrogen bonds. Taking into
account that deprotonation represents a critical step in coordinate bonds formation, the
protonation of an additional N atom may represent a key difference between the interactions
of BV with copper in different solvents.",
publisher = "COST Action CA15133",
journal = "Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland",
title = "Structure of biliverdin and its interaction with copper",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3187"
}

Dimitrijević, M., Bogdanović Pristov, J., Žižić, M., Stanković, D., Bajuk-Bogdanović, D., Stanić, M., Hagen, W., Piccioli, M.,& Spasojević, I.. (2019). Structure of biliverdin and its interaction with copper. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
COST Action CA15133., 44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3187

Dimitrijević M, Bogdanović Pristov J, Žižić M, Stanković D, Bajuk-Bogdanović D, Stanić M, Hagen W, Piccioli M, Spasojević I. Structure of biliverdin and its interaction with copper. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. 2019;:44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3187 .

Dimitrijević, Milena, Bogdanović Pristov, Jelena, Žižić, Milan, Stanković, Dalibor, Bajuk-Bogdanović, Danica, Stanić, Marina, Hagen, Wilfred, Piccioli, Mario, Spasojević, Ivan, "Structure of biliverdin and its interaction with copper" in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland (2019):44-44,
https://hdl.handle.net/21.15107/rcub_rimsi_3187 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2418
AB  - Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
T1  - Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus
EP  - 118
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2418
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetic Antic, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2019",
abstract = "Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia",
title = "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2418"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetic Antic, T., Živić, M.,& Stanić, M.. (2019). Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetic Antic T, Živić M, Stanić M. Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia. 2019;:118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetic Antic, Tijana, Živić, Miroslav, Stanić, Marina, "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus" in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia (2019):118-118,
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

TY  - JOUR
AU  - Dimitrijević, Milena
AU  - Bogdanović Pristov, Jelena
AU  - Žižić, Milan
AU  - Stanković, Dalibor M.
AU  - Bajuk-Bogdanović, Danica
AU  - Stanić, Marina
AU  - Spasic, Snežana
AU  - Hagen, Wilfred
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1246
AB  - Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, H-1 NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1:1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e(-). The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O-2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Biliverdin-copper complex at physiological pH
EP  - 6070
IS  - 18
SP  - 6061
VL  - 48
DO  - 10.1039/c8dt04724c
ER  - 

@article{
author = "Dimitrijević, Milena and Bogdanović Pristov, Jelena and Žižić, Milan and Stanković, Dalibor M. and Bajuk-Bogdanović, Danica and Stanić, Marina and Spasic, Snežana and Hagen, Wilfred and Spasojević, Ivan",
year = "2019",
abstract = "Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, H-1 NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1:1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e(-). The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O-2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Biliverdin-copper complex at physiological pH",
pages = "6070-6061",
number = "18",
volume = "48",
doi = "10.1039/c8dt04724c"
}

Dimitrijević, M., Bogdanović Pristov, J., Žižić, M., Stanković, D. M., Bajuk-Bogdanović, D., Stanić, M., Spasic, S., Hagen, W.,& Spasojević, I.. (2019). Biliverdin-copper complex at physiological pH. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 48(18), 6061-6070.
https://doi.org/10.1039/c8dt04724c

Dimitrijević M, Bogdanović Pristov J, Žižić M, Stanković DM, Bajuk-Bogdanović D, Stanić M, Spasic S, Hagen W, Spasojević I. Biliverdin-copper complex at physiological pH. in Dalton Transactions. 2019;48(18):6061-6070.
doi:10.1039/c8dt04724c .

Dimitrijević, Milena, Bogdanović Pristov, Jelena, Žižić, Milan, Stanković, Dalibor M., Bajuk-Bogdanović, Danica, Stanić, Marina, Spasic, Snežana, Hagen, Wilfred, Spasojević, Ivan, "Biliverdin-copper complex at physiological pH" in Dalton Transactions, 48, no. 18 (2019):6061-6070,
https://doi.org/10.1039/c8dt04724c . .