TY  - CONF
AU  - Prokopijević, Miloš
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2377
AB  - Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.
PB  - East Sarajevo, Faculty of Agriculture
C3  - XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina
T1  - CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS
EP  - 214
SP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2377
ER  - 

@conference{
author = "Prokopijević, Miloš and Bartolić, Dragana and Stanković, Mira and Radotić, Ksenija",
year = "2023",
abstract = "Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.",
publisher = "East Sarajevo, Faculty of Agriculture",
journal = "XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina",
title = "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS",
pages = "214-214",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2377"
}

Prokopijević, M., Bartolić, D., Stanković, M.,& Radotić, K.. (2023). CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina
East Sarajevo, Faculty of Agriculture., 214-214.
https://hdl.handle.net/21.15107/rcub_rimsi_2377

Prokopijević M, Bartolić D, Stanković M, Radotić K. CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina. 2023;:214-214.
https://hdl.handle.net/21.15107/rcub_rimsi_2377 .

Prokopijević, Miloš, Bartolić, Dragana, Stanković, Mira, Radotić, Ksenija, "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS" in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina (2023):214-214,
https://hdl.handle.net/21.15107/rcub_rimsi_2377 .

TY  - GEN
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Bartolić, Dragana
AU  - Stevanovic, Jevrosima
AU  - Andrić, Filip
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2366
AB  - Honey bees have a very important role in pollinating plants, in addition to honey production. Bee
diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.
PB  - University of Belgrade, Technical Faculty in Bor
T2  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING
EP  - 46
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2366
ER  - 

@misc{
author = "Stanković, Mira and Prokopijević, Miloš and Bartolić, Dragana and Stevanovic, Jevrosima and Andrić, Filip and Radotić, Ksenija",
year = "2023",
abstract = "Honey bees have a very important role in pollinating plants, in addition to honey production. Bee
diseases are one of the reasons of a significant decrease in bee colonies in the world in recent years. Honey samples originating from hives with different infestations with N. ceranae were analyzed. Total phenolic content and total protein content were determined by the Folin-Ciocalteu assay and the Bradford assay, respectively. Fluorescence spectroscopy combined with PARAFAC was used to determine the spectral components originating from proteins (PFSC1) and phenolics (PFSC2) in honey samples, and their ratios were calculated. Phenols and proteins content in the honey samples, obtained by spectrophotometric quantification, decreases with increasing infestation levels in the respective hives infected with N. ceranae. A negative correlation was obtained for the ratio of PARAFAC components PFSC2/PFSC1 and the level of infestation with N. ceranae in honey samples. These results indicate that fluorescence spectroscopy combined with PARAFAC could be used as an optical tool for non-invasive and rapid screening of honey to estimate bee health status.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING",
pages = "46-40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2366"
}

Stanković, M., Prokopijević, M., Bartolić, D., Stevanovic, J., Andrić, F.,& Radotić, K.. (2023). ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 40-46.
https://hdl.handle.net/21.15107/rcub_rimsi_2366

Stanković M, Prokopijević M, Bartolić D, Stevanovic J, Andrić F, Radotić K. ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:40-46.
https://hdl.handle.net/21.15107/rcub_rimsi_2366 .

Stanković, Mira, Prokopijević, Miloš, Bartolić, Dragana, Stevanovic, Jevrosima, Andrić, Filip, Radotić, Ksenija, "ADVANCED OPTICAL TOOLS APPLIED ON HONEY SAMPLES FOR BEE HEALTH STATUS MONITORING" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):40-46,
https://hdl.handle.net/21.15107/rcub_rimsi_2366 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1999
AB  - Environmental safety may be compromised by the presence of hazardous chemical compounds, such as bisphenol A (BPA), which is commonly used in the production of certain types of plastics. BPA is an emerging organic contaminant that could be found in many matrices, such as drinking water, due to anthropogenic activities. Within this study, we used fluorescence spectroscopy to analyse the photoluminescent characteristics of BPA in various solutions and drinking water. The emission spectra of BPA in various solvents are recorded from 280 nm to 380 nm, after excitation wavelength at 230 nm. These results imply that the fluorescence approach can be used for rapid detection and estimation of the level of BPA in water samples and, hence, for non-invasive monitoring of BPA in drinking water is an important concern for public health and environmental protection.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - Fluorescence characterisation of bisphenol A in various solvents and drinking water
EP  - 305
SP  - 302
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1999
ER  - 

@conference{
author = "Prokopijević, Miloš and Stanković, Mira and Bartolić, Dragana and Mitrović, Aleksandra Lj. and Radotić, Ksenija",
year = "2023",
abstract = "Environmental safety may be compromised by the presence of hazardous chemical compounds, such as bisphenol A (BPA), which is commonly used in the production of certain types of plastics. BPA is an emerging organic contaminant that could be found in many matrices, such as drinking water, due to anthropogenic activities. Within this study, we used fluorescence spectroscopy to analyse the photoluminescent characteristics of BPA in various solutions and drinking water. The emission spectra of BPA in various solvents are recorded from 280 nm to 380 nm, after excitation wavelength at 230 nm. These results imply that the fluorescence approach can be used for rapid detection and estimation of the level of BPA in water samples and, hence, for non-invasive monitoring of BPA in drinking water is an important concern for public health and environmental protection.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "Fluorescence characterisation of bisphenol A in various solvents and drinking water",
pages = "305-302",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1999"
}

Prokopijević, M., Stanković, M., Bartolić, D., Mitrović, A. Lj.,& Radotić, K.. (2023). Fluorescence characterisation of bisphenol A in various solvents and drinking water. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 302-305.
https://hdl.handle.net/21.15107/rcub_rimsi_1999

Prokopijević M, Stanković M, Bartolić D, Mitrović AL, Radotić K. Fluorescence characterisation of bisphenol A in various solvents and drinking water. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:302-305.
https://hdl.handle.net/21.15107/rcub_rimsi_1999 .

Prokopijević, Miloš, Stanković, Mira, Bartolić, Dragana, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Fluorescence characterisation of bisphenol A in various solvents and drinking water" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):302-305,
https://hdl.handle.net/21.15107/rcub_rimsi_1999 .

TY  - GEN
AU  - Bartolić, Dragana
AU  - Lucev, Milica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Algara, Manuel
AU  - Stanković, Slavica
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2374
AB  - Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.
PB  - University of Belgrade, Technical Faculty in Bor
T2  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus
EP  - 54
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2374
ER  - 

@misc{
author = "Bartolić, Dragana and Lucev, Milica and Stanković, Mira and Prokopijević, Miloš and Algara, Manuel and Stanković, Slavica and Radotić, Ksenija",
year = "2023",
abstract = "Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus",
pages = "54-47",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2374"
}

Bartolić, D., Lucev, M., Stanković, M., Prokopijević, M., Algara, M., Stanković, S.,& Radotić, K.. (2023). ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374

Bartolić D, Lucev M, Stanković M, Prokopijević M, Algara M, Stanković S, Radotić K. ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

Bartolić, Dragana, Lucev, Milica, Stanković, Mira, Prokopijević, Miloš, Algara, Manuel, Stanković, Slavica, Radotić, Ksenija, "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):47-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

TY  - JOUR
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Sikoparija, Branko
AU  - Nedic, Nebojsa
AU  - Andric, Filip
AU  - Natalija, Polović
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1781
AB  - Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.
PB  - MDPI
T2  - Foods
T1  - Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies
EP  - 13
SP  - 629
SP  - 1
VL  - 12
DO  - 10.3390/foods12030629
ER  - 

@article{
author = "Stanković, Mira and Prokopijević, Miloš and Sikoparija, Branko and Nedic, Nebojsa and Andric, Filip and Natalija, Polović and Natić, Maja and Radotić, Ksenija",
year = "2023",
abstract = "Varroa destructor is a parasitic mite responsible for the loss of honey bee (Apis mellifera)
colonies. This study aimed to find a promising marker in honey for the bee colony infestation level
using fluorescence spectroscopy and biochemical analyses. We examined whether the parameters of
the honey samples’ fluorescence spectra and biochemical parameters, both related to proteins and
phenolics, may be connected with the level of honey bee colonies’ infestation. The infestation level
was highly positively correlated with the catalase activity in honey (r = 0.936). Additionally, the
infestation level was positively correlated with the phenolic spectral component (r = 0.656), which
was tentatively related to the phenolics in honey. No correlation was found between the diastase
activity in honey and the colonies’ infestation level. The results indicate that the catalase activity in
honey and the PFC1 spectral component may be reliable markers for the V. destructor infestation level
of the colonies. The obtained data may be related to the honey yield obtained from the apiaries.",
publisher = "MDPI",
journal = "Foods",
title = "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies",
pages = "13-629-1",
volume = "12",
doi = "10.3390/foods12030629"
}

Stanković, M., Prokopijević, M., Sikoparija, B., Nedic, N., Andric, F., Natalija, P., Natić, M.,& Radotić, K.. (2023). Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods
MDPI., 12, 629-13.
https://doi.org/10.3390/foods12030629

Stanković M, Prokopijević M, Sikoparija B, Nedic N, Andric F, Natalija P, Natić M, Radotić K. Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies. in Foods. 2023;12:629-13.
doi:10.3390/foods12030629 .

Stanković, Mira, Prokopijević, Miloš, Sikoparija, Branko, Nedic, Nebojsa, Andric, Filip, Natalija, Polović, Natić, Maja, Radotić, Ksenija, "Using Front-Face Fluorescence Spectroscopy and Biochemical Analysis of Honey to Assess a Marker for the Level of Varroa destructor Infestation of Honey Bee (Apis mellifera) Colonies" in Foods, 12 (2023):629-13,
https://doi.org/10.3390/foods12030629 . .

TY  - CONF
AU  - Pantić, Nevena
AU  - Spasojević, Milica
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Balaž, Ana Marija
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1774
AB  - For the purpose of immobilization, one of the most commonly used enzymes is horseradish peroxidase (HRP). Different carriers can be used as supports for the immobilization of HRP: alginate, pectin, magnetic-beads, macroporous copolymers, silicas etc. Covalent binding of an enzyme to the carrier leads to the formation of strong linkage, thus preventing the enzyme leakage. Macroporous copolymers with different porous characteristics were used for the immobilization of horseradish peroxidase by employing periodate and glutaraldehyde method. Five and 25 mg of HRP were immobilized per gram of the copolymer. Increasing the amount of added enzyme leads to the increase of specific activity of immobilized enzyme. Copolymer with the pore diameter of 297 nm showed the most promising results in terms of specific activity. Immobilized enzymes can be used for the removal of phenolic compounds from waste effluents.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research
T1  - Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal
EP  - 359
SP  - 354
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1774
ER  - 

@conference{
author = "Pantić, Nevena and Spasojević, Milica and Prokopijević, Miloš and Spasojević, Dragica and Balaž, Ana Marija and Prodanović, Radivoje and Prodanović, Olivera",
year = "2022",
abstract = "For the purpose of immobilization, one of the most commonly used enzymes is horseradish peroxidase (HRP). Different carriers can be used as supports for the immobilization of HRP: alginate, pectin, magnetic-beads, macroporous copolymers, silicas etc. Covalent binding of an enzyme to the carrier leads to the formation of strong linkage, thus preventing the enzyme leakage. Macroporous copolymers with different porous characteristics were used for the immobilization of horseradish peroxidase by employing periodate and glutaraldehyde method. Five and 25 mg of HRP were immobilized per gram of the copolymer. Increasing the amount of added enzyme leads to the increase of specific activity of immobilized enzyme. Copolymer with the pore diameter of 297 nm showed the most promising results in terms of specific activity. Immobilized enzymes can be used for the removal of phenolic compounds from waste effluents.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research",
title = "Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal",
pages = "359-354",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1774"
}

Pantić, N., Spasojević, M., Prokopijević, M., Spasojević, D., Balaž, A. M., Prodanović, R.,& Prodanović, O.. (2022). Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal. in 29th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 354-359.
https://hdl.handle.net/21.15107/rcub_rimsi_1774

Pantić N, Spasojević M, Prokopijević M, Spasojević D, Balaž AM, Prodanović R, Prodanović O. Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal. in 29th International Conference Ecological Truth and Environmental Research. 2022;:354-359.
https://hdl.handle.net/21.15107/rcub_rimsi_1774 .

Pantić, Nevena, Spasojević, Milica, Prokopijević, Miloš, Spasojević, Dragica, Balaž, Ana Marija, Prodanović, Radivoje, Prodanović, Olivera, "Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal" in 29th International Conference Ecological Truth and Environmental Research (2022):354-359,
https://hdl.handle.net/21.15107/rcub_rimsi_1774 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1767
AB  - Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
T1  - PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP
EP  - 363
SP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1767
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Stanković, Mira and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research - EcoTER'22",
title = "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP",
pages = "363-360",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1767"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Stanković, M., Radotić, K.,& Prodanović, R.. (2022). PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
University of Belgrade, Technical Faculty in Bor., 360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Stanković M, Radotić K, Prodanović R. PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22. 2022;:360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Stanković, Mira, Radotić, Ksenija, Prodanović, Radivoje, "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP" in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 (2022):360-363,
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Bartolić, Dragana
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1770
AB  - The application of enzymes for phenol removal from polluted waters is an effective and
environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive
enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic
compounds in the presence of H2O2. The industrial application, however, requires enzyme
immobilization on various carriers to overcome the disadvantages of using the soluble form.
Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional
polymeric network. Immobilization of SBP was performed in an emulsion polymerization
reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of
micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate.
In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios
was tested to determine the carrier with the best performance. Immobilized peroxidase has potential
for application as a biocatalyst for phenol removal from wastewater.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research
T1  - Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin
EP  - 353
SP  - 350
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1770
ER  - 

@conference{
author = "Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Olivera and Pantić, Nevena and Bartolić, Dragana and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "The application of enzymes for phenol removal from polluted waters is an effective and
environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive
enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic
compounds in the presence of H2O2. The industrial application, however, requires enzyme
immobilization on various carriers to overcome the disadvantages of using the soluble form.
Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional
polymeric network. Immobilization of SBP was performed in an emulsion polymerization
reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of
micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate.
In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios
was tested to determine the carrier with the best performance. Immobilized peroxidase has potential
for application as a biocatalyst for phenol removal from wastewater.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research",
title = "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin",
pages = "353-350",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1770"
}

Prokopijević, M., Spasojević, D., Prodanović, O., Pantić, N., Bartolić, D., Radotić, K.,& Prodanović, R.. (2022). Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 350-353.
https://hdl.handle.net/21.15107/rcub_rimsi_1770

Prokopijević M, Spasojević D, Prodanović O, Pantić N, Bartolić D, Radotić K, Prodanović R. Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research. 2022;:350-353.
https://hdl.handle.net/21.15107/rcub_rimsi_1770 .

Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Olivera, Pantić, Nevena, Bartolić, Dragana, Radotić, Ksenija, Prodanović, Radivoje, "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin" in 29th International Conference Ecological Truth and Environmental Research (2022):350-353,
https://hdl.handle.net/21.15107/rcub_rimsi_1770 .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Mutavdžić, Dragosav
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1766
AB  - Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood. Tension wood contains more cellulose and less lignin and it is anatomically different from normal wood. Fourier-transform infrared (FTIR) spectroscopy showed that there was no difference in lignin content in segments of bent trees compared to the controls, but the higher amounts of cellulose, xylan, and xyloglucan were observed in bent trees, corresponding to the rearrangements in the cellulose/xyloglucan network.
PB  - Society of Physical Chemists of Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA
EP  - 298
SP  - 295
VL  - I
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1766
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Mutavdžić, Dragosav and Spasojević, Dragica and Prokopijević, Miloš and Radotić, Ksenija and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood. Tension wood contains more cellulose and less lignin and it is anatomically different from normal wood. Fourier-transform infrared (FTIR) spectroscopy showed that there was no difference in lignin content in segments of bent trees compared to the controls, but the higher amounts of cellulose, xylan, and xyloglucan were observed in bent trees, corresponding to the rearrangements in the cellulose/xyloglucan network.",
publisher = "Society of Physical Chemists of Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA",
pages = "298-295",
volume = "I",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1766"
}

Simonović Radosavljević, J., Mutavdžić, D., Spasojević, D., Prokopijević, M., Radotić, K.,& Mitrović, A. Lj.. (2022). FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., I, 295-298.
https://hdl.handle.net/21.15107/rcub_rimsi_1766

Simonović Radosavljević J, Mutavdžić D, Spasojević D, Prokopijević M, Radotić K, Mitrović AL. FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;I:295-298.
https://hdl.handle.net/21.15107/rcub_rimsi_1766 .

Simonović Radosavljević, Jasna, Mutavdžić, Dragosav, Spasojević, Dragica, Prokopijević, Miloš, Radotić, Ksenija, Mitrović, Aleksandra Lj., "FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, I (2022):295-298,
https://hdl.handle.net/21.15107/rcub_rimsi_1766 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1780
AB  - Semena kukuruza (Zea mais L.) mogu biti kontaminirana sa jednim od najpasnijih prirodnih kancerogenih kontaminanata kao što su aflatoksini. U ovoj studiji, proučavan je uticaj aflateritoksina na strukturne karakteristike strukture. Korišćena je fluorescentna spektropija u kombinaciji sa matematičkom analizom spektra. Primenjen je matematički model za analizu spektra asimetričnog modela (Log-normal) koji koristi Nelder-Mead algoritam (Matlab 6.5). Rezultati razlaganja fluorescentnih spektara pokazuju četiri glavne fluorescentne fluorescentne emisije sa 054 emisiona približna 050 komponenta. Ovaj rezultat može da se koristi za praćenje promene u semenu usled prisustva aflatoksina. Glavni pokazatelj uticaja aflatoksina na strukturu kukuružnog semena je pomeraj dugo-talasne emisione komponente (520 nm).
PB  - Srpsko biološko društvo, Beograd, 2022.
C3  - Treći kongres biologa Srbije
T1  - Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom
SP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1780
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2022",
abstract = "Semena kukuruza (Zea mais L.) mogu biti kontaminirana sa jednim od najpasnijih prirodnih kancerogenih kontaminanata kao što su aflatoksini. U ovoj studiji, proučavan je uticaj aflateritoksina na strukturne karakteristike strukture. Korišćena je fluorescentna spektropija u kombinaciji sa matematičkom analizom spektra. Primenjen je matematički model za analizu spektra asimetričnog modela (Log-normal) koji koristi Nelder-Mead algoritam (Matlab 6.5). Rezultati razlaganja fluorescentnih spektara pokazuju četiri glavne fluorescentne fluorescentne emisije sa 054 emisiona približna 050 komponenta. Ovaj rezultat može da se koristi za praćenje promene u semenu usled prisustva aflatoksina. Glavni pokazatelj uticaja aflatoksina na strukturu kukuružnog semena je pomeraj dugo-talasne emisione komponente (520 nm).",
publisher = "Srpsko biološko društvo, Beograd, 2022.",
journal = "Treći kongres biologa Srbije",
title = "Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom",
pages = "21",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1780"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D., Kalauzi, A.,& Radotić, K.. (2022). Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom. in Treći kongres biologa Srbije
Srpsko biološko društvo, Beograd, 2022.., 21.
https://hdl.handle.net/21.15107/rcub_rimsi_1780

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Kalauzi A, Radotić K. Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom. in Treći kongres biologa Srbije. 2022;:21.
https://hdl.handle.net/21.15107/rcub_rimsi_1780 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Radotić, Ksenija, "Primena fluorescentne spektroskopije u kombinaciji sa metodom dekonvolucije u analizi semena kukuruza (Zea mays L.) kontaminiranih aflatoksinom" in Treći kongres biologa Srbije (2022):21,
https://hdl.handle.net/21.15107/rcub_rimsi_1780 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1699
AB  - Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY
SP  - 342
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1699
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Varroa infestation is considered a major threat to honeybees due to its strong impact on the colony, attacking larval cells, increasing bee mortality, and serving as a vector of pathogens. Coumaphos is an organophosphate-based acaricide insecticide which is frequently used to control Varroa (Varroa destructor) mites in the honey bee (Apis melifera) colonies. The accumulation of these lipophilic contaminants in honey and beeswax may affect honey bee health, mainly honey bee larvae which are the most sensitive, and negatively affect queen quality. In this study, we analyzed honey samples from hives of honey bees that were treated with Coumaphos, as well as the samples of pure Coumafos. The front-face fluorescence measurements of analyed honey samples were recorded using an Fl3-221 P spectrofluorimeter, with a 450 W Xenon lamp. Obtained results showed an emission peak with maximum around 400 nm, after excitation at 340 nm. It could be related to the presence of Coumaphos in honey samples, which showed the characteristic position of emission maximum at the same wavelength. These results showed that fluorescence spectroscopy may be useful as a fast and sensitive method, for the efficient and specific determination of toxic substances such as Coumaphos residues in honey. Due to the proven toxic effects on honey bees, as well as on humans, monitoring hazardous contaminants in honey is of crucial importance in the field of food and agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY",
pages = "342",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1699"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:342.
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "DETERMINATION OF COUMAPHOS RESIDUES IN HONEY AFTER VARROA TREATMENT USING FLUORESCENCE SPECTROSCOPY" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):342,
https://hdl.handle.net/21.15107/rcub_rimsi_1699 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Đikanović, Daniela
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Jovanović, Jelena
AU  - Dragišić Maksimović, Jelena
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1704
AB  - Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE
SP  - 341
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1704
ER  - 

@conference{
author = "Bartolić, Dragana and Đikanović, Daniela and Stanković, Mira and Prokopijević, Miloš and Jovanović, Jelena and Dragišić Maksimović, Jelena and Radotić, Ksenija",
year = "2022",
abstract = "Silica is beneficial element for plants. Many studies of silica show the useful effects that Si can provide on plant-environment relationships in a wide variety of crops, as increase in growth and yield and improvement of resistance to metal toxicity, salt stress, drought resistance etc. The occurrence of Si within a plant is a result of its uptake in the form of soluble Si(OH)4 or Si(OH)3O ion from the soil, and its polymerization at a final location. Si is mostly accumulated in the cell walls (CWs), thus providing strengthening of CWs, increasing mechanical strength, and alleviating effects of various biotic and abiotic types of stress. Lignin, as one of the main components of the plant CW, is a natural phenolic polymer with high molecular weight and complex structure. Accumulation of silica in plants is directly connected with chemistry and structure of lignin. Silicon affects the composition of CWs by its binding to the phenolic and carbohydrate CW components in polymerization reactions by altering linkages of non-cellulosic polymers and lignin. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase catalyzed polymerization of a lignin monomer into the lignin model compound (DHP). Fluorescence microscopy and spectroscopy combined with AFM technique showed that Si was bound to the final polymer, and the structure of the Si-DHP differed from pure DHP. We show that Si significantly influence on lignin structure. The results contribute to understanding of Si binding to lignin in cell walls, which may have impact on possible Si applications in agriculture.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE",
pages = "341",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1704"
}

Bartolić, D., Đikanović, D., Stanković, M., Prokopijević, M., Jovanović, J., Dragišić Maksimović, J.,& Radotić, K.. (2022). INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704

Bartolić D, Đikanović D, Stanković M, Prokopijević M, Jovanović J, Dragišić Maksimović J, Radotić K. INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:341.
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

Bartolić, Dragana, Đikanović, Daniela, Stanković, Mira, Prokopijević, Miloš, Jovanović, Jelena, Dragišić Maksimović, Jelena, Radotić, Ksenija, "INVESTIGATION OF Si-LIGNIN INTERACTION BY FLUORESCENCE TECHNIQUES AND ATOMIC FORCE MICROSCOPY- POSSIBLE APPLICATION IN AGRICULTURE" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):341,
https://hdl.handle.net/21.15107/rcub_rimsi_1704 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1700
AB  - Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.
PB  - East Sarajevo : Faculty of Agriculture, 2022
C3  - XIII International Scientific Agriculture Symposium “AGROSYM 2022”
T1  - BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1700
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Mung bean (Vigna Radiata L.) seeds are an important source of both nutrients (such as proteins, fibers, vitamins) and a variety of bioactive compounds (like phenolic compounds). Ultraviolet (UV) light has an important function as a major environmental signal important for plant growth and development, but at the same time it may cause certain damaging effects on macromolecules and other cellular components. Plants respond to the production of reactive oxygen species (ROS), caused by UV irradiation, by activating changes in morphology, physiology, or production of secondary metabolites. Some of these mechanisms increase antioxidant capacity in order to reduce the harmful effect of produced ROS. In the present research, the duration of exposure to UV-A irradiation and its influence on antioxidant activity of mung bean seeds were studied. Seeds were exposed to constant irradiation for 1 or 3 hours using a UV-A lamp (93μW/cm2) at a 50 cm distance. Antioxidant activity was tested using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The results indicate a significant increase in antioxidant activity of the UV-A irradiated seeds after 1 hour (74,45 % ± 0,40), compared to the control (72,85 % ± 1,55). After prolonged exposure, measured antioxidant activity significantly increased (77,99 % ± 0,71). Our results show a correlation between duration of UV-A irradiation and the increase in antioxidant activity. This could be beneficial in agriculture for producing fortified food.",
publisher = "East Sarajevo : Faculty of Agriculture, 2022",
journal = "XIII International Scientific Agriculture Symposium “AGROSYM 2022”",
title = "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS",
pages = "203",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1700"
}

Bartolić, D., Stanković, M., Prokopijević, M., Đikanović, D.,& Radotić, K.. (2022). BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”
East Sarajevo : Faculty of Agriculture, 2022., 203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700

Bartolić D, Stanković M, Prokopijević M, Đikanović D, Radotić K. BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS. in XIII International Scientific Agriculture Symposium “AGROSYM 2022”. 2022;:203.
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Đikanović, Daniela, Radotić, Ksenija, "BENEFICIAL EFFECTS OF UV-A RADIATION ON MUNG BEAN (VIGNA RADIATA L.) SEEDS" in XIII International Scientific Agriculture Symposium “AGROSYM 2022” (2022):203,
https://hdl.handle.net/21.15107/rcub_rimsi_1700 .

TY  - JOUR
AU  - Prokopijević, Miloš
AU  - Simonović Radosavljević, Jasna
AU  - Spasojević, Dragica
AU  - Vojisavljević, Katarina
AU  - Radotić, Ksenija
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1773
AB  - Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.
PB  - De Gruyter
T2  - Holzforschung
T1  - XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending
EP  - 2505
IS  - 6
SP  - 2500
VL  - 76
DO  - 10.1515/hf-2021-0223
ER  - 

@article{
author = "Prokopijević, Miloš and Simonović Radosavljević, Jasna and Spasojević, Dragica and Vojisavljević, Katarina and Radotić, Ksenija and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.",
publisher = "De Gruyter",
journal = "Holzforschung",
title = "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending",
pages = "2505-2500",
number = "6",
volume = "76",
doi = "10.1515/hf-2021-0223"
}

Prokopijević, M., Simonović Radosavljević, J., Spasojević, D., Vojisavljević, K., Radotić, K.,& Mitrović, A. Lj.. (2022). XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung
De Gruyter., 76(6), 2500-2505.
https://doi.org/10.1515/hf-2021-0223

Prokopijević M, Simonović Radosavljević J, Spasojević D, Vojisavljević K, Radotić K, Mitrović AL. XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung. 2022;76(6):2500-2505.
doi:10.1515/hf-2021-0223 .

Prokopijević, Miloš, Simonović Radosavljević, Jasna, Spasojević, Dragica, Vojisavljević, Katarina, Radotić, Ksenija, Mitrović, Aleksandra Lj., "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending" in Holzforschung, 76, no. 6 (2022):2500-2505,
https://doi.org/10.1515/hf-2021-0223 . .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1765
AB  - Mung bean (Vigna radiata L.) is a leguminous plant with high nutritional value, traditionally known as a functional food. Legume seeds are a rich source of proteins, vitamins, minerals, and essential amino acids but also contain bioactive components and polyphenols which possess a high antioxidant capacity. Pigments content (chlorophyll a and b, carotenoids) was determined as good parameter for estimation of seed quality and an indicator of tolerance to different types of stress. The antioxidant activity of the seeds was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. The concentration of chlorophile a and b and carotenoids were determined by a spectrophotometric method. Obtained results indicate a higher content of chlorophyll a than chlorophyll b, 0.352 μg/ml and 0.220 μg/ml respectively, while total carotenoids content was 0.108 μg/ml and DPPH radical scavenging activity was 54.52% ± 1.77. The advancement in this research lies in collecting information about bioactive compounds, such as chlorophylls and carotenoids, that are useful in improving the functional and antioxidant properties of quality seeds used in daily diet.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022
T1  - Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content
SP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1765
ER  - 

@conference{
author = "Bartolić, Dragana and Prokopijević, Miloš and Stanković, Mira and Radotić, Ksenija",
year = "2022",
abstract = "Mung bean (Vigna radiata L.) is a leguminous plant with high nutritional value, traditionally known as a functional food. Legume seeds are a rich source of proteins, vitamins, minerals, and essential amino acids but also contain bioactive components and polyphenols which possess a high antioxidant capacity. Pigments content (chlorophyll a and b, carotenoids) was determined as good parameter for estimation of seed quality and an indicator of tolerance to different types of stress. The antioxidant activity of the seeds was determined using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. The concentration of chlorophile a and b and carotenoids were determined by a spectrophotometric method. Obtained results indicate a higher content of chlorophyll a than chlorophyll b, 0.352 μg/ml and 0.220 μg/ml respectively, while total carotenoids content was 0.108 μg/ml and DPPH radical scavenging activity was 54.52% ± 1.77. The advancement in this research lies in collecting information about bioactive compounds, such as chlorophylls and carotenoids, that are useful in improving the functional and antioxidant properties of quality seeds used in daily diet.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022",
title = "Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1765"
}

Bartolić, D., Prokopijević, M., Stanković, M.,& Radotić, K.. (2022). Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš; Institute for Nature Conservation of Serbia, Belgrade., 202.
https://hdl.handle.net/21.15107/rcub_rimsi_1765

Bartolić D, Prokopijević M, Stanković M, Radotić K. Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022. 2022;:202.
https://hdl.handle.net/21.15107/rcub_rimsi_1765 .

Bartolić, Dragana, Prokopijević, Miloš, Stanković, Mira, Radotić, Ksenija, "Characterization of Mung bean (Vigna radiata L.) seeds: antioxidant activity, chlorophyll and carotenoid content" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, Serbia, June 2022 (2022):202,
https://hdl.handle.net/21.15107/rcub_rimsi_1765 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1772
AB  - Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.
PB  - Pleiades Publishing, Ltd.
T2  - Russian Journal of Physical Chemistry A
T1  - Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds
EP  - 2800
IS  - 12
SP  - 2797
VL  - 96
DO  - 10.1134/S0036024422120044
ER  - 

@article{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija",
year = "2022",
abstract = "Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.",
publisher = "Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds",
pages = "2800-2797",
number = "12",
volume = "96",
doi = "10.1134/S0036024422120044"
}

Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2022). Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A
Pleiades Publishing, Ltd.., 96(12), 2797-2800.
https://doi.org/10.1134/S0036024422120044

Bartolić D, Stanković M, Prokopijević M, Radotić K. Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A. 2022;96(12):2797-2800.
doi:10.1134/S0036024422120044 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds" in Russian Journal of Physical Chemistry A, 96, no. 12 (2022):2797-2800,
https://doi.org/10.1134/S0036024422120044 . .

TY  - GEN
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2879
AB  - У последњих 20 година, расте интересовање за биополимере, не само међу биохемичарима и молекуларним биолозима, него и у другим научним областима, а посебно у области науке о материјалима. Главне предности биополимера у односу на полимере из фосилних горива, јесу јефтино добијање из обновљивих извора и биоразградивост. Полисахариди су најзаступљенија класа биополимера у природи. Целулоза је главна структурна компонента ћелијског зида виших биљака и по количини најобилнији биополимер на земљи. За њом следи хемицелулоза, која представља скуп хетерополисахарида различите структуре. Најзаступљенији морски полисахарид је алгинат, конституент ћелијског зида мрких морских алги. Полисахариди поседују бројне функционалне групе, погодне за различите врсте хемијских модификација. Јонизабилне групе обезбеђују им хидрофилност, као и способност везивања наелектрисаних молекула и јона. Све ове особине допринеле су да полисахариди постану одличан материјал за производњу хидрогелова. Хидрогелови на бази биополимера нашли су своју примену у индустрији, пољопривреди, фармацији, медицини, заштити животне средине...
PB  - Српско биолошко друштво, Београд
T2  - Treći kongres biologa Srbije
T1  - Biljni polisaharidi kao hidrogelovi
SP  - 281
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2879
ER  - 

@misc{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "У последњих 20 година, расте интересовање за биополимере, не само међу биохемичарима и молекуларним биолозима, него и у другим научним областима, а посебно у области науке о материјалима. Главне предности биополимера у односу на полимере из фосилних горива, јесу јефтино добијање из обновљивих извора и биоразградивост. Полисахариди су најзаступљенија класа биополимера у природи. Целулоза је главна структурна компонента ћелијског зида виших биљака и по количини најобилнији биополимер на земљи. За њом следи хемицелулоза, која представља скуп хетерополисахарида различите структуре. Најзаступљенији морски полисахарид је алгинат, конституент ћелијског зида мрких морских алги. Полисахариди поседују бројне функционалне групе, погодне за различите врсте хемијских модификација. Јонизабилне групе обезбеђују им хидрофилност, као и способност везивања наелектрисаних молекула и јона. Све ове особине допринеле су да полисахариди постану одличан материјал за производњу хидрогелова. Хидрогелови на бази биополимера нашли су своју примену у индустрији, пољопривреди, фармацији, медицини, заштити животне средине...",
publisher = "Српско биолошко друштво, Београд",
journal = "Treći kongres biologa Srbije",
title = "Biljni polisaharidi kao hidrogelovi",
pages = "281",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2879"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Radotić, K.,& Prodanović, R.. (2022). Biljni polisaharidi kao hidrogelovi. in Treći kongres biologa Srbije
Српско биолошко друштво, Београд., 281.
https://hdl.handle.net/21.15107/rcub_rimsi_2879

Spasojević D, Prokopijević M, Prodanović O, Radotić K, Prodanović R. Biljni polisaharidi kao hidrogelovi. in Treći kongres biologa Srbije. 2022;:281.
https://hdl.handle.net/21.15107/rcub_rimsi_2879 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Radotić, Ksenija, Prodanović, Radivoje, "Biljni polisaharidi kao hidrogelovi" in Treći kongres biologa Srbije (2022):281,
https://hdl.handle.net/21.15107/rcub_rimsi_2879 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1544
AB  - BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study
EP  - 2505
IS  - 6
SP  - 2500
VL  - 102
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baosic, Rada and Radotić, Ksenija",
year = "2022",
abstract = "BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study",
pages = "2505-2500",
number = "6",
volume = "102",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baosic, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baosic R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baosic, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1759
AB  - INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.
PB  - Prirodno-matematički fakultet Novi Sad
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
T1  - Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads
EP  - 133
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1759
ER  - 

@conference{
author = "Spasojević, Dragica and Stanković, Mira and Prokopijević, Miloš and Prodanović, Olivera and Stojkovska, Jasmina and Obradović, Bojana and Radotić, Ksenija",
year = "2021",
abstract = "INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.",
publisher = "Prirodno-matematički fakultet Novi Sad",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021",
title = "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads",
pages = "133-132",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1759"
}

Spasojević, D., Stanković, M., Prokopijević, M., Prodanović, O., Stojkovska, J., Obradović, B.,& Radotić, K.. (2021). Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
Prirodno-matematički fakultet Novi Sad., 132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759

Spasojević D, Stanković M, Prokopijević M, Prodanović O, Stojkovska J, Obradović B, Radotić K. Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021. 2021;:132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

Spasojević, Dragica, Stanković, Mira, Prokopijević, Miloš, Prodanović, Olivera, Stojkovska, Jasmina, Obradović, Bojana, Radotić, Ksenija, "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads" in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021 (2021):132-133,
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1790
AB  - Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.
PB  - Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization
EP  - 342
SP  - 340
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1790
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Radotić, Ksenija and Prodanović, Radivoje",
year = "2021",
abstract = "Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.",
publisher = "Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization",
pages = "342-340",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1790"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Radotić, K.,& Prodanović, R.. (2021). Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 1, 340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Radotić K, Prodanović R. Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;1:340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Radotić, Ksenija, Prodanović, Radivoje, "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2021):340-342,
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović Radosavljević, Jasna
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Kovacević, Jovana
AU  - Prodanović, Olivera
AU  - Todorović, Bratislav
AU  - Matović, Branko
AU  - Stanković, Mira
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Skocic, Milos
AU  - Pesic, Mirjana
AU  - Prokic, Ljiljana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1449
AB  - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Cell wall response to UV radiation in needles of Picea omorika
EP  - 190
SP  - 176
VL  - 161
DO  - 10.1016/j.plaphy.2021.02.007
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovacević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skocic, Milos and Pesic, Mirjana and Prokic, Ljiljana and Radotić, Ksenija",
year = "2021",
abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Cell wall response to UV radiation in needles of Picea omorika",
pages = "190-176",
volume = "161",
doi = "10.1016/j.plaphy.2021.02.007"
}

Mitrović, A. Lj., Simonović Radosavljević, J., Prokopijević, M., Spasojević, D., Kovacević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skocic, M., Pesic, M., Prokic, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 161, 176-190.
https://doi.org/10.1016/j.plaphy.2021.02.007

Mitrović AL, Simonović Radosavljević J, Prokopijević M, Spasojević D, Kovacević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skocic M, Pesic M, Prokic L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190.
doi:10.1016/j.plaphy.2021.02.007 .

Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovacević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skocic, Milos, Pesic, Mirjana, Prokic, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190,
https://doi.org/10.1016/j.plaphy.2021.02.007 . .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1795
AB  - UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.
PB  - Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS
EP  - 339
SP  - 336
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1795
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija",
year = "2021",
abstract = "UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.",
publisher = "Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS",
pages = "339-336",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1795"
}

Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2021). INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 336-339.
https://hdl.handle.net/21.15107/rcub_rimsi_1795

Bartolić D, Stanković M, Prokopijević M, Radotić K. INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;:336-339.
https://hdl.handle.net/21.15107/rcub_rimsi_1795 .

Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2021):336-339,
https://hdl.handle.net/21.15107/rcub_rimsi_1795 .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Stanković, Mira
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1762
AB  - Application of fluorescence spectroscopy combined with chemometrics algorithms provides rapid and non-destructive screening method in seed quality estimation, widely used in the agricultural industry and crop breeding. Fluorescence spectroscopy is a technique capable of detecting differs fluorophores among various colored maize seed cultivars and through different seed fractions. In the present study, we used the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) algorithm to analyse the excitation-emission matrices (EEMs) of various cultivars of colored maize (Zea mays L.) seeds and its fractions. The EEMs were recorded as a set, with the excitation ranging from 280 nm to 330 nm and the emission spectra ranging from 300 nm to 550 nm. The MCR-ALS analysis yielded two major fluorescence components for all of the analysed samples. Both position and shape of component 1 (C1) varied among the samples. On the other hand, the position and shape were similar for component 2 (C2). C1 could be used as a marker for the discrimination of colored seeds and their fractions. The observed variations in C1 between the analysed seeds may be due to the presence of their individual fluorophores, assigned to anthocyanins, proteins, and phenolics. In conclusion, the MCR-ALS analysis of the seed emission spectra has a great potential for the rapid and non-expensive characterization of various cultivars of colored seeds.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
T1  - Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis
EP  - 746
SP  - 742
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1762
ER  - 

@conference{
author = "Bartolić, Dragana and Prokopijević, Miloš and Stanković, Mira and Radotić, Ksenija",
year = "2021",
abstract = "Application of fluorescence spectroscopy combined with chemometrics algorithms provides rapid and non-destructive screening method in seed quality estimation, widely used in the agricultural industry and crop breeding. Fluorescence spectroscopy is a technique capable of detecting differs fluorophores among various colored maize seed cultivars and through different seed fractions. In the present study, we used the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) algorithm to analyse the excitation-emission matrices (EEMs) of various cultivars of colored maize (Zea mays L.) seeds and its fractions. The EEMs were recorded as a set, with the excitation ranging from 280 nm to 330 nm and the emission spectra ranging from 300 nm to 550 nm. The MCR-ALS analysis yielded two major fluorescence components for all of the analysed samples. Both position and shape of component 1 (C1) varied among the samples. On the other hand, the position and shape were similar for component 2 (C2). C1 could be used as a marker for the discrimination of colored seeds and their fractions. The observed variations in C1 between the analysed seeds may be due to the presence of their individual fluorophores, assigned to anthocyanins, proteins, and phenolics. In conclusion, the MCR-ALS analysis of the seed emission spectra has a great potential for the rapid and non-expensive characterization of various cultivars of colored seeds.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina",
title = "Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis",
pages = "746-742",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1762"
}

Bartolić, D., Prokopijević, M., Stanković, M.,& Radotić, K.. (2021). Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis. in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 742-746.
https://hdl.handle.net/21.15107/rcub_rimsi_1762

Bartolić D, Prokopijević M, Stanković M, Radotić K. Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis. in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina. 2021;:742-746.
https://hdl.handle.net/21.15107/rcub_rimsi_1762 .

Bartolić, Dragana, Prokopijević, Miloš, Stanković, Mira, Radotić, Ksenija, "Characterization of colored maize seed fractions using fluorescence spectroscopy and multivariate analysis" in Book of Proceedings: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina (2021):742-746,
https://hdl.handle.net/21.15107/rcub_rimsi_1762 .

TY  - JOUR
AU  - Miličević, Ivana
AU  - Hadzima-Nyarko, Marijana
AU  - Bušić, Robert
AU  - Simonović Radosavljević, Jasna
AU  - Prokopijević, Miloš
AU  - Vojisavljević, Katarina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1805
AB  - This paper investigates the effects of different
treatment methods of rubber aggregates for self-compacting concrete
(SCC) on compressive strength and modulus of elasticity. SCC
mixtures with 10% replacement of fine aggregate with crumb rubber
by total aggregate volume and with different aggregate treatment
methods were investigated. The rubber aggregate was treated in three
different methods: dry process, water-soaking, and NaOH treatment
plus water soaking. Properties of SCC in a fresh and hardened state
were tested and evaluated. Scanning electron microscope (SEM)
analysis of three different SCC patches were made and discussed. It
was observed that applying the proposed NaOH plus water soaking
method resulted in the improvement of fresh and hardened concrete
properties. It resulted in a more uniform distribution of rubber
particles in the cement matrix, a better bond between rubber particles
and the cement matrix, and higher compressive strength of SCC
rubberized concrete.
PB  - World Academy of Science, Engineering and Technology
T2  - International Journal of Structural and Construction Engineering
T1  - Effect of rubber treatment on compressive strength and modulus of elasticity of self- compacting rubberized concrete
EP  - 134
IS  - 3
SP  - 131
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1805
ER  - 

@article{
author = "Miličević, Ivana and Hadzima-Nyarko, Marijana and Bušić, Robert and Simonović Radosavljević, Jasna and Prokopijević, Miloš and Vojisavljević, Katarina",
year = "2021",
abstract = "This paper investigates the effects of different
treatment methods of rubber aggregates for self-compacting concrete
(SCC) on compressive strength and modulus of elasticity. SCC
mixtures with 10% replacement of fine aggregate with crumb rubber
by total aggregate volume and with different aggregate treatment
methods were investigated. The rubber aggregate was treated in three
different methods: dry process, water-soaking, and NaOH treatment
plus water soaking. Properties of SCC in a fresh and hardened state
were tested and evaluated. Scanning electron microscope (SEM)
analysis of three different SCC patches were made and discussed. It
was observed that applying the proposed NaOH plus water soaking
method resulted in the improvement of fresh and hardened concrete
properties. It resulted in a more uniform distribution of rubber
particles in the cement matrix, a better bond between rubber particles
and the cement matrix, and higher compressive strength of SCC
rubberized concrete.",
publisher = "World Academy of Science, Engineering and Technology",
journal = "International Journal of Structural and Construction Engineering",
title = "Effect of rubber treatment on compressive strength and modulus of elasticity of self- compacting rubberized concrete",
pages = "134-131",
number = "3",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1805"
}

Miličević, I., Hadzima-Nyarko, M., Bušić, R., Simonović Radosavljević, J., Prokopijević, M.,& Vojisavljević, K.. (2021). Effect of rubber treatment on compressive strength and modulus of elasticity of self- compacting rubberized concrete. in International Journal of Structural and Construction Engineering
World Academy of Science, Engineering and Technology., 15(3), 131-134.
https://hdl.handle.net/21.15107/rcub_rimsi_1805

Miličević I, Hadzima-Nyarko M, Bušić R, Simonović Radosavljević J, Prokopijević M, Vojisavljević K. Effect of rubber treatment on compressive strength and modulus of elasticity of self- compacting rubberized concrete. in International Journal of Structural and Construction Engineering. 2021;15(3):131-134.
https://hdl.handle.net/21.15107/rcub_rimsi_1805 .

Miličević, Ivana, Hadzima-Nyarko, Marijana, Bušić, Robert, Simonović Radosavljević, Jasna, Prokopijević, Miloš, Vojisavljević, Katarina, "Effect of rubber treatment on compressive strength and modulus of elasticity of self- compacting rubberized concrete" in International Journal of Structural and Construction Engineering, 15, no. 3 (2021):131-134,
https://hdl.handle.net/21.15107/rcub_rimsi_1805 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Prokopijević, Miloš
AU  - Živanović, Branka  D.
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1800
AB  - Maize is the most commonly consumed cereal in the world. Its various compounds are associated with nutraceutical properties and health-promoting benefits. Therefore, maize seeds are considered to be functional food with high antioxidant activities. The drying conditions have a huge impact on maintaining the seed quality. This study aimed to evaluate the effect of temperature on the antioxidant activities of white and red maize (Zea mays L.) cultivars. A drying time of 1 h at a temperature of 45 °C was tested. The antioxidant activity expressed in percentages was evaluated as radical scavenging activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent. The results revealed that the antioxidant activity of the dried white seeds (74.08 %) was not significantly different (p>0.005) with respect to the un-dried seeds used as control (72.6 %). Contrary, drying at 45 °C caused a significant (p<0.005) rise of antioxidant activity in the red-coloured seeds' being 85.95 % and 92.25 % before and after the treatment, respectively. The obtained results show that the antioxidant activity may be a reliable indicator for the estimation of the cereal seeds’ quality, which is useful in the food industry and agriculture.
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
T2  - Book of Abstracts: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
T1  - Effect of drying temperature on antioxidant activity of white and red maize (Zea mays L.) seeds
SP  - 198
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1800
ER  - 

@article{
author = "Bartolić, Dragana and Prokopijević, Miloš and Živanović, Branka  D. and Radotić, Ksenija",
year = "2021",
abstract = "Maize is the most commonly consumed cereal in the world. Its various compounds are associated with nutraceutical properties and health-promoting benefits. Therefore, maize seeds are considered to be functional food with high antioxidant activities. The drying conditions have a huge impact on maintaining the seed quality. This study aimed to evaluate the effect of temperature on the antioxidant activities of white and red maize (Zea mays L.) cultivars. A drying time of 1 h at a temperature of 45 °C was tested. The antioxidant activity expressed in percentages was evaluated as radical scavenging activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent. The results revealed that the antioxidant activity of the dried white seeds (74.08 %) was not significantly different (p>0.005) with respect to the un-dried seeds used as control (72.6 %). Contrary, drying at 45 °C caused a significant (p<0.005) rise of antioxidant activity in the red-coloured seeds' being 85.95 % and 92.25 % before and after the treatment, respectively. The obtained results show that the antioxidant activity may be a reliable indicator for the estimation of the cereal seeds’ quality, which is useful in the food industry and agriculture.",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Abstracts: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina",
title = "Effect of drying temperature on antioxidant activity of white and red maize (Zea mays L.) seeds",
pages = "198",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1800"
}

Bartolić, D., Prokopijević, M., Živanović, Branka  D.,& Radotić, K.. (2021). Effect of drying temperature on antioxidant activity of white and red maize (Zea mays L.) seeds. in Book of Abstracts: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 198.
https://hdl.handle.net/21.15107/rcub_rimsi_1800

Bartolić D, Prokopijević M, Živanović, Branka  D., Radotić K. Effect of drying temperature on antioxidant activity of white and red maize (Zea mays L.) seeds. in Book of Abstracts: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina. 2021;:198.
https://hdl.handle.net/21.15107/rcub_rimsi_1800 .

Bartolić, Dragana, Prokopijević, Miloš, Živanović, Branka  D., Radotić, Ksenija, "Effect of drying temperature on antioxidant activity of white and red maize (Zea mays L.) seeds" in Book of Abstracts: XII International Scientific Agriculture Symposium "AGROSYM 2021", October 7-10, 2021, Jahorina, Bosnia and Herzegovina (2021):198,
https://hdl.handle.net/21.15107/rcub_rimsi_1800 .