Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin
Аутори
Prokopijević, MilošSpasojević, Dragica
Prodanović, Olivera
Pantić, Nevena
Bartolić, Dragana
Radotić, Ksenija
Prodanović, Radivoje
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The application of enzymes for phenol removal from polluted waters is an effective and
environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive
enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic
compounds in the presence of H2O2. The industrial application, however, requires enzyme
immobilization on various carriers to overcome the disadvantages of using the soluble form.
Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional
polymeric network. Immobilization of SBP was performed in an emulsion polymerization
reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of
micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate.
In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios
was tested to determine the carrier with the best per...formance. Immobilized peroxidase has potential
for application as a biocatalyst for phenol removal from wastewater.
Кључне речи:
immobilization / soybean peroxidase / pectin / tyramine / hydrogelИзвор:
29th International Conference Ecological Truth and Environmental Research, 2022, 350-353Издавач:
- University of Belgrade, Technical Faculty in Bor
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Prokopijević, Miloš AU - Spasojević, Dragica AU - Prodanović, Olivera AU - Pantić, Nevena AU - Bartolić, Dragana AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1770 AB - The application of enzymes for phenol removal from polluted waters is an effective and environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic compounds in the presence of H2O2. The industrial application, however, requires enzyme immobilization on various carriers to overcome the disadvantages of using the soluble form. Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional polymeric network. Immobilization of SBP was performed in an emulsion polymerization reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate. In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios was tested to determine the carrier with the best performance. Immobilized peroxidase has potential for application as a biocatalyst for phenol removal from wastewater. PB - University of Belgrade, Technical Faculty in Bor C3 - 29th International Conference Ecological Truth and Environmental Research T1 - Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin EP - 353 SP - 350 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1770 ER -
@conference{ author = "Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Olivera and Pantić, Nevena and Bartolić, Dragana and Radotić, Ksenija and Prodanović, Radivoje", year = "2022", abstract = "The application of enzymes for phenol removal from polluted waters is an effective and environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic compounds in the presence of H2O2. The industrial application, however, requires enzyme immobilization on various carriers to overcome the disadvantages of using the soluble form. Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional polymeric network. Immobilization of SBP was performed in an emulsion polymerization reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate. In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios was tested to determine the carrier with the best performance. Immobilized peroxidase has potential for application as a biocatalyst for phenol removal from wastewater.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "29th International Conference Ecological Truth and Environmental Research", title = "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin", pages = "353-350", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1770" }
Prokopijević, M., Spasojević, D., Prodanović, O., Pantić, N., Bartolić, D., Radotić, K.,& Prodanović, R.. (2022). Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research University of Belgrade, Technical Faculty in Bor., 350-353. https://hdl.handle.net/21.15107/rcub_rimsi_1770
Prokopijević M, Spasojević D, Prodanović O, Pantić N, Bartolić D, Radotić K, Prodanović R. Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research. 2022;:350-353. https://hdl.handle.net/21.15107/rcub_rimsi_1770 .
Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Olivera, Pantić, Nevena, Bartolić, Dragana, Radotić, Ksenija, Prodanović, Radivoje, "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin" in 29th International Conference Ecological Truth and Environmental Research (2022):350-353, https://hdl.handle.net/21.15107/rcub_rimsi_1770 .