CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS
Апстракт
Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed th...e presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.
Кључне речи:
Mung bean / fluorescence spectroscopy / MCR-ALS / TPC / DPPHИзвор:
XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina, 2023, 214-214Издавач:
- East Sarajevo, Faculty of Agriculture
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Prokopijević, Miloš AU - Bartolić, Dragana AU - Stanković, Mira AU - Radotić, Ksenija PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2377 AB - Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food. PB - East Sarajevo, Faculty of Agriculture C3 - XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina T1 - CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS EP - 214 SP - 214 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2377 ER -
@conference{ author = "Prokopijević, Miloš and Bartolić, Dragana and Stanković, Mira and Radotić, Ksenija", year = "2023", abstract = "Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.", publisher = "East Sarajevo, Faculty of Agriculture", journal = "XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina", title = "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS", pages = "214-214", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2377" }
Prokopijević, M., Bartolić, D., Stanković, M.,& Radotić, K.. (2023). CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina East Sarajevo, Faculty of Agriculture., 214-214. https://hdl.handle.net/21.15107/rcub_rimsi_2377
Prokopijević M, Bartolić D, Stanković M, Radotić K. CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS. in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina. 2023;:214-214. https://hdl.handle.net/21.15107/rcub_rimsi_2377 .
Prokopijević, Miloš, Bartolić, Dragana, Stanković, Mira, Radotić, Ksenija, "CHARACTERISATION OF MUNG BEAN (VIGNA RADIATA L.) SEEDS USING FLUORESCENCE SPECTROSCOPY AND MULTIVARIATE ANALYIS" in XIV International Scientific Agriculture Symposium "Agrosym 2023", Jahorina (2023):214-214, https://hdl.handle.net/21.15107/rcub_rimsi_2377 .