TY  - CONF
AU  - Milenković, Ivana
AU  - Zhou, Yiqun
AU  - Spasić, Slađana
AU  - Leblanc, Roger M.
AU  - Borisev, Milan
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1845
AB  - INTRODUCTION:
Carbon dots (CDs) are considered a green alternative to metal nanoparticles because they can be used where metal nanoparticles cannot be applied. Orange carbon dots (CDs), synthesized from citric acid and o-phenylenediamine as precursors, are organic spherical nanoparticles with a lot of applications in various biomedical purposes such as drug delivery, bioimaging, and sensing. Ease of preparation, high photoluminescence, solubility in water, and biocompatibility are their main advantages.
OBJECTIVES:
The main aim of this research was to investigate the effect of oCDs on total phenolic activity (TPC) in maize as an agricultural species. TPC reflects the contribution of phenolics as a group of secondary metabolites participating in the regulation of plant growth and in the defense responses. Also, it is one of the main indicators of oxidative stress which can cause a metabolic disorder in plants.
METHOD / DESIGN:
Three different concentrations (1, 5, and 10 mg L-1) of oCDs nanoparticles were used for the treatment of maize plants via KNOP/2 hydroponic solution during 2 week-growth under 16 h/8 h photoperiod. TPC was analyzed from phenolic extracts obtained from the roots and leaves of plants after foliar and solution treatments. Folin-Ciocalteu’s spectrophotometric procedure was used for the determination of TPC in the samples.
RESULTS:
The results showed that foliar applications with all concentrations of o-CDs induced decreases of TPC in maize leaves but did not affect these parameters in the roots. In solution treatment, the concentration of 1 mg/L of o-CDs increased TPC in the leaves, but decreased in roots.
treatment
CONCLUSIONS:
The higher efficiency achieved with the lowest o-CD concentration (1 mg/L) in foliar treatment makes this way of application advantageous compared with the solution counterpart.
PB  - University of Novi Sad, Faculty of Sciences, Serbia
C3  - International BioScience Conference and the 8th International PSU – UNS Bioscience Conference
T1  - Orange carbon dots change the total phenolic content in maize
EP  - 130
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1845
ER  - 

@conference{
author = "Milenković, Ivana and Zhou, Yiqun and Spasić, Slađana and Leblanc, Roger M. and Borisev, Milan and Radotić, Ksenija",
year = "2021",
abstract = "INTRODUCTION:
Carbon dots (CDs) are considered a green alternative to metal nanoparticles because they can be used where metal nanoparticles cannot be applied. Orange carbon dots (CDs), synthesized from citric acid and o-phenylenediamine as precursors, are organic spherical nanoparticles with a lot of applications in various biomedical purposes such as drug delivery, bioimaging, and sensing. Ease of preparation, high photoluminescence, solubility in water, and biocompatibility are their main advantages.
OBJECTIVES:
The main aim of this research was to investigate the effect of oCDs on total phenolic activity (TPC) in maize as an agricultural species. TPC reflects the contribution of phenolics as a group of secondary metabolites participating in the regulation of plant growth and in the defense responses. Also, it is one of the main indicators of oxidative stress which can cause a metabolic disorder in plants.
METHOD / DESIGN:
Three different concentrations (1, 5, and 10 mg L-1) of oCDs nanoparticles were used for the treatment of maize plants via KNOP/2 hydroponic solution during 2 week-growth under 16 h/8 h photoperiod. TPC was analyzed from phenolic extracts obtained from the roots and leaves of plants after foliar and solution treatments. Folin-Ciocalteu’s spectrophotometric procedure was used for the determination of TPC in the samples.
RESULTS:
The results showed that foliar applications with all concentrations of o-CDs induced decreases of TPC in maize leaves but did not affect these parameters in the roots. In solution treatment, the concentration of 1 mg/L of o-CDs increased TPC in the leaves, but decreased in roots.
treatment
CONCLUSIONS:
The higher efficiency achieved with the lowest o-CD concentration (1 mg/L) in foliar treatment makes this way of application advantageous compared with the solution counterpart.",
publisher = "University of Novi Sad, Faculty of Sciences, Serbia",
journal = "International BioScience Conference and the 8th International PSU – UNS Bioscience Conference",
title = "Orange carbon dots change the total phenolic content in maize",
pages = "130-129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1845"
}

Milenković, I., Zhou, Y., Spasić, S., Leblanc, R. M., Borisev, M.,& Radotić, K.. (2021). Orange carbon dots change the total phenolic content in maize. in International BioScience Conference and the 8th International PSU – UNS Bioscience Conference
University of Novi Sad, Faculty of Sciences, Serbia., 129-130.
https://hdl.handle.net/21.15107/rcub_rimsi_1845

Milenković I, Zhou Y, Spasić S, Leblanc RM, Borisev M, Radotić K. Orange carbon dots change the total phenolic content in maize. in International BioScience Conference and the 8th International PSU – UNS Bioscience Conference. 2021;:129-130.
https://hdl.handle.net/21.15107/rcub_rimsi_1845 .

Milenković, Ivana, Zhou, Yiqun, Spasić, Slađana, Leblanc, Roger M., Borisev, Milan, Radotić, Ksenija, "Orange carbon dots change the total phenolic content in maize" in International BioScience Conference and the 8th International PSU – UNS Bioscience Conference (2021):129-130,
https://hdl.handle.net/21.15107/rcub_rimsi_1845 .

TY  - CONF
AU  - Milenković, Ivana
AU  - Zhou, Yiqun
AU  - Spasić, Slađana
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1852
AB  - Carbon dots (CDs) are spherical organic nanoparticles with a huge potential for application in various biomedical purposes such as sensing, bioimaging, and drug delivery, as well as in water remediation. The discovery of CDs was very popular in the 21th century because they can be used where metal nanoparticles cannot be applied, so they are considered as their green alternative. The main advantages of these nanoparticles are their ease of preparation, solubility in water, high photoluminescence, and biocompatibility. Having all these properties in mind, the main aim of this research was to investigate the effect of orange carbon dots (oCDs), synthesized from citric acid and o-phenylenediamine as precursors, on total antioxidative activity (TAA) in maize as a model plant and agricultural species. We tested antioxidative activity as an indicator of oxidative stress and metabolic disorder in plants. TAA mainly includes the contribution of different non-enzymatic components with antioxidant capacity (vitamins, phenolic acids, sugars, etc.). The maize plants were exposed to three different concentrations (1, 5, and 10 mg L-1) of oCDs nanoparticles via KNOP/2 hydroponic solution during growth under 16 h/8 h photoperiod. After the 2 week-growth,  the leaves and roots of plants were separately harvested and collected. The results showed that oCDs did not induce any change in TAA in the leaves, but increased TAA in roots after the treatment of plants with 5 and 10 mg L-1 oCDs. It can be concluded that plant treatment with oCDs’ concentrations ≥ 5 mg L-1 leads to the increase of oxidative stress in roots as a plant organ more exposed to the nanoparticles. This research opens new possibilities in the uses of oCDs in agricultural applications.
PB  - Maize Research Institute, Zemun Polje
C3  - International Conference ”The Frontiers of Science and Technology in Crop Breeding and Production“
T1  - Influence of orange carbon dots on antioxidative activity in maize
EP  - 57
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1852
ER  - 

@conference{
author = "Milenković, Ivana and Zhou, Yiqun and Spasić, Slađana and Leblanc, Roger M. and Radotić, Ksenija",
year = "2021",
abstract = "Carbon dots (CDs) are spherical organic nanoparticles with a huge potential for application in various biomedical purposes such as sensing, bioimaging, and drug delivery, as well as in water remediation. The discovery of CDs was very popular in the 21th century because they can be used where metal nanoparticles cannot be applied, so they are considered as their green alternative. The main advantages of these nanoparticles are their ease of preparation, solubility in water, high photoluminescence, and biocompatibility. Having all these properties in mind, the main aim of this research was to investigate the effect of orange carbon dots (oCDs), synthesized from citric acid and o-phenylenediamine as precursors, on total antioxidative activity (TAA) in maize as a model plant and agricultural species. We tested antioxidative activity as an indicator of oxidative stress and metabolic disorder in plants. TAA mainly includes the contribution of different non-enzymatic components with antioxidant capacity (vitamins, phenolic acids, sugars, etc.). The maize plants were exposed to three different concentrations (1, 5, and 10 mg L-1) of oCDs nanoparticles via KNOP/2 hydroponic solution during growth under 16 h/8 h photoperiod. After the 2 week-growth,  the leaves and roots of plants were separately harvested and collected. The results showed that oCDs did not induce any change in TAA in the leaves, but increased TAA in roots after the treatment of plants with 5 and 10 mg L-1 oCDs. It can be concluded that plant treatment with oCDs’ concentrations ≥ 5 mg L-1 leads to the increase of oxidative stress in roots as a plant organ more exposed to the nanoparticles. This research opens new possibilities in the uses of oCDs in agricultural applications.",
publisher = "Maize Research Institute, Zemun Polje",
journal = "International Conference ”The Frontiers of Science and Technology in Crop Breeding and Production“",
title = "Influence of orange carbon dots on antioxidative activity in maize",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1852"
}

Milenković, I., Zhou, Y., Spasić, S., Leblanc, R. M.,& Radotić, K.. (2021). Influence of orange carbon dots on antioxidative activity in maize. in International Conference ”The Frontiers of Science and Technology in Crop Breeding and Production“
Maize Research Institute, Zemun Polje., 57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_1852

Milenković I, Zhou Y, Spasić S, Leblanc RM, Radotić K. Influence of orange carbon dots on antioxidative activity in maize. in International Conference ”The Frontiers of Science and Technology in Crop Breeding and Production“. 2021;:57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_1852 .

Milenković, Ivana, Zhou, Yiqun, Spasić, Slađana, Leblanc, Roger M., Radotić, Ksenija, "Influence of orange carbon dots on antioxidative activity in maize" in International Conference ”The Frontiers of Science and Technology in Crop Breeding and Production“ (2021):57-57,
https://hdl.handle.net/21.15107/rcub_rimsi_1852 .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Borisev, Milan
AU  - Zhou, Yiqun
AU  - Spasić, Slađana
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1431
AB  - The sustained increase in leaf photosynthesis may increase crop yield. Due to many limitations, plants use much less photosynthetic capacity than is theoretically possible. Plant nanobionics investigates nanoparticle application in living plants, which improves certain plant functions. We synthesized and tested nontoxic orange carbon dots (o-CDs) for the photosynthetic efficiency increase in maize (Zea mays L.). We applied o-CDs foliarly or by adding to the growth solution. The photosynthetic parameters and content of photosynthetic pigments were recorded. The total phenolic content (TPC) and total antioxidant activity (TAA) were measured to monitor the plant antioxidant response to o-CDs. The photosynthetic parameters' values were higher for foliar than for solution application. The 1 mg/L o-CDs applied foliarly and 5 mg/L in solution increased photosynthetic parameters in leaves. The o-CDs raised photosynthetic pigments. The TAA and TPC results indicate reduced antioxidant activity in the plant organs more exposed to o-CDs, depending on the way of application.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Photosynthesis Enhancement in Maize via Nontoxic Orange Carbon Dots
EP  - 5451
IS  - 19
SP  - 5446
VL  - 69
DO  - 10.1021/acs.jafc.1c01094
ER  - 

@article{
author = "Milenković, Ivana and Borisev, Milan and Zhou, Yiqun and Spasić, Slađana and Leblanc, Roger M. and Radotić, Ksenija",
year = "2021",
abstract = "The sustained increase in leaf photosynthesis may increase crop yield. Due to many limitations, plants use much less photosynthetic capacity than is theoretically possible. Plant nanobionics investigates nanoparticle application in living plants, which improves certain plant functions. We synthesized and tested nontoxic orange carbon dots (o-CDs) for the photosynthetic efficiency increase in maize (Zea mays L.). We applied o-CDs foliarly or by adding to the growth solution. The photosynthetic parameters and content of photosynthetic pigments were recorded. The total phenolic content (TPC) and total antioxidant activity (TAA) were measured to monitor the plant antioxidant response to o-CDs. The photosynthetic parameters' values were higher for foliar than for solution application. The 1 mg/L o-CDs applied foliarly and 5 mg/L in solution increased photosynthetic parameters in leaves. The o-CDs raised photosynthetic pigments. The TAA and TPC results indicate reduced antioxidant activity in the plant organs more exposed to o-CDs, depending on the way of application.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Photosynthesis Enhancement in Maize via Nontoxic Orange Carbon Dots",
pages = "5451-5446",
number = "19",
volume = "69",
doi = "10.1021/acs.jafc.1c01094"
}

Milenković, I., Borisev, M., Zhou, Y., Spasić, S., Leblanc, R. M.,& Radotić, K.. (2021). Photosynthesis Enhancement in Maize via Nontoxic Orange Carbon Dots. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 69(19), 5446-5451.
https://doi.org/10.1021/acs.jafc.1c01094

Milenković I, Borisev M, Zhou Y, Spasić S, Leblanc RM, Radotić K. Photosynthesis Enhancement in Maize via Nontoxic Orange Carbon Dots. in Journal of Agricultural and Food Chemistry. 2021;69(19):5446-5451.
doi:10.1021/acs.jafc.1c01094 .

Milenković, Ivana, Borisev, Milan, Zhou, Yiqun, Spasić, Slađana, Leblanc, Roger M., Radotić, Ksenija, "Photosynthesis Enhancement in Maize via Nontoxic Orange Carbon Dots" in Journal of Agricultural and Food Chemistry, 69, no. 19 (2021):5446-5451,
https://doi.org/10.1021/acs.jafc.1c01094 . .

TY  - GEN
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Donaldson, Lloyd
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1654
AB  - Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.
PB  - 5th International Congress on Microscopy & Spectroscopy
T2  - 5th International Congress on Microscopy & Spectroscopy
T1  - Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1654
ER  - 

@misc{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Donaldson, Lloyd and Leblanc, Roger M. and Radotić, Ksenija",
year = "2018",
abstract = "Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.",
publisher = "5th International Congress on Microscopy & Spectroscopy",
journal = "5th International Congress on Microscopy & Spectroscopy",
title = "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1654"
}

Đikanović, D., Kalauzi, A., Donaldson, L., Leblanc, R. M.,& Radotić, K.. (2018). Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy
5th International Congress on Microscopy & Spectroscopy., 82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654

Đikanović D, Kalauzi A, Donaldson L, Leblanc RM, Radotić K. Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy. 2018;:82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

Đikanović, Daniela, Kalauzi, Aleksandar, Donaldson, Lloyd, Leblanc, Roger M., Radotić, Ksenija, "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls" in 5th International Congress on Microscopy & Spectroscopy (2018):82,
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melo, Thor Bernt
AU  - Leblanc, Roger M.
AU  - Yousef, Yaser A.
AU  - Naqvi, K. Razi
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/988
AB  - To interpret accurately protein fluorescence and phosphorescence, it is essential to achieve a better understanding of the luminescence properties of tryptophan (Trp, or W) in peptides. In published literature data on luminescence of peptides of varied length are scarce. This article describes studies of fluorescence and phosphorescence properties of the eight Trp-containing synthetic peptides: WAK, AWK, SWA, KYLWE, AVSWK, WVSWAK, WAKLAWE, and AVSWAKLARE. The aim was to investigate which factors influence the fluorescence yield and phosphorescence-spectra and lifetimes. Absorption spectra, room temperature fluorescence emission and corresponding excitation spectra and time-resolved phosphorescence spectra (77 K) have been recorded; the dependence of the fluorescence quantum yield on the specific peptide and its variation with the wavelength of excitation has been studied. The changes in fluorescence yield and shape of phosphorescence spectra are explained in terms of intemal electron and proton transfer. The structured phosphorescence spectrum originates from proton transfer occurring upon excitation of Trp, while electron transfer gives rise to a non-structured luminescence spectrum. There is also electron transfer from higher vibronic S-1 states. In the peptides there is higher probability of electron transfer than in Trp alone. The obtained data are interpreted in light of the peptides' sequence, length and conformation.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence
EP  - 128
SP  - 120
VL  - 157
DO  - 10.1016/j.jphotobiol.2016.02.011
ER  - 

@article{
author = "Radotić, Ksenija and Melo, Thor Bernt and Leblanc, Roger M. and Yousef, Yaser A. and Naqvi, K. Razi",
year = "2016",
abstract = "To interpret accurately protein fluorescence and phosphorescence, it is essential to achieve a better understanding of the luminescence properties of tryptophan (Trp, or W) in peptides. In published literature data on luminescence of peptides of varied length are scarce. This article describes studies of fluorescence and phosphorescence properties of the eight Trp-containing synthetic peptides: WAK, AWK, SWA, KYLWE, AVSWK, WVSWAK, WAKLAWE, and AVSWAKLARE. The aim was to investigate which factors influence the fluorescence yield and phosphorescence-spectra and lifetimes. Absorption spectra, room temperature fluorescence emission and corresponding excitation spectra and time-resolved phosphorescence spectra (77 K) have been recorded; the dependence of the fluorescence quantum yield on the specific peptide and its variation with the wavelength of excitation has been studied. The changes in fluorescence yield and shape of phosphorescence spectra are explained in terms of intemal electron and proton transfer. The structured phosphorescence spectrum originates from proton transfer occurring upon excitation of Trp, while electron transfer gives rise to a non-structured luminescence spectrum. There is also electron transfer from higher vibronic S-1 states. In the peptides there is higher probability of electron transfer than in Trp alone. The obtained data are interpreted in light of the peptides' sequence, length and conformation.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence",
pages = "128-120",
volume = "157",
doi = "10.1016/j.jphotobiol.2016.02.011"
}

Radotić, K., Melo, T. B., Leblanc, R. M., Yousef, Y. A.,& Naqvi, K. R.. (2016). Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 157, 120-128.
https://doi.org/10.1016/j.jphotobiol.2016.02.011

Radotić K, Melo TB, Leblanc RM, Yousef YA, Naqvi KR. Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. in Journal of Photochemistry and Photobiology B-Biology. 2016;157:120-128.
doi:10.1016/j.jphotobiol.2016.02.011 .

Radotić, Ksenija, Melo, Thor Bernt, Leblanc, Roger M., Yousef, Yaser A., Naqvi, K. Razi, "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence" in Journal of Photochemistry and Photobiology B-Biology, 157 (2016):120-128,
https://doi.org/10.1016/j.jphotobiol.2016.02.011 . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melo, Thor Bernt
AU  - Leblanc, Roger M.
AU  - Yousef, Yaser A.
AU  - Naqvi, K. Razi
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/979
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence (vol 157, pg 120, 2016)
EP  - 281
SP  - 281
VL  - 158
DO  - 10.1016/j.jphotobiol.2016.03.034
ER  - 

@article{
author = "Radotić, Ksenija and Melo, Thor Bernt and Leblanc, Roger M. and Yousef, Yaser A. and Naqvi, K. Razi",
year = "2016",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence (vol 157, pg 120, 2016)",
pages = "281-281",
volume = "158",
doi = "10.1016/j.jphotobiol.2016.03.034"
}

Radotić, K., Melo, T. B., Leblanc, R. M., Yousef, Y. A.,& Naqvi, K. R.. (2016). Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence (vol 157, pg 120, 2016). in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 158, 281-281.
https://doi.org/10.1016/j.jphotobiol.2016.03.034

Radotić K, Melo TB, Leblanc RM, Yousef YA, Naqvi KR. Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence (vol 157, pg 120, 2016). in Journal of Photochemistry and Photobiology B-Biology. 2016;158:281-281.
doi:10.1016/j.jphotobiol.2016.03.034 .

Radotić, Ksenija, Melo, Thor Bernt, Leblanc, Roger M., Yousef, Yaser A., Naqvi, K. Razi, "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence (vol 157, pg 120, 2016)" in Journal of Photochemistry and Photobiology B-Biology, 158 (2016):281-281,
https://doi.org/10.1016/j.jphotobiol.2016.03.034 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Jeremic, Milorad G
AU  - Xu, Jianmin
AU  - Micic, Miodrag
AU  - Whyte, Jeffrey D
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/521
AB  - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of the CdSe quantum dots with plant cell walls
EP  - 47
SP  - 41
VL  - 91
DO  - 10.1016/j.colsurfb.2011.10.032
ER  - 

@article{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Jeremic, Milorad G and Xu, Jianmin and Micic, Miodrag and Whyte, Jeffrey D and Leblanc, Roger M. and Radotić, Ksenija",
year = "2012",
abstract = "There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of the CdSe quantum dots with plant cell walls",
pages = "47-41",
volume = "91",
doi = "10.1016/j.colsurfb.2011.10.032"
}

Đikanović, D., Kalauzi, A., Jeremic, M. G., Xu, J., Micic, M., Whyte, J. D., Leblanc, R. M.,& Radotić, K.. (2012). Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 91, 41-47.
https://doi.org/10.1016/j.colsurfb.2011.10.032

Đikanović D, Kalauzi A, Jeremic MG, Xu J, Micic M, Whyte JD, Leblanc RM, Radotić K. Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces. 2012;91:41-47.
doi:10.1016/j.colsurfb.2011.10.032 .

Đikanović, Daniela, Kalauzi, Aleksandar, Jeremic, Milorad G, Xu, Jianmin, Micic, Miodrag, Whyte, Jeffrey D, Leblanc, Roger M., Radotić, Ksenija, "Interaction of the CdSe quantum dots with plant cell walls" in Colloids and Surfaces B-Biointerfaces, 91 (2012):41-47,
https://doi.org/10.1016/j.colsurfb.2011.10.032 . .

TY  - JOUR
AU  - Mutavdžić, Dragosav
AU  - Xu, Jianmin
AU  - Thakur, Garima
AU  - Triulzi, Robert
AU  - Kasas, Sandor
AU  - Jeremic, Milorad G
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/481
AB  - There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding fluorescence spectrum. It has been found that fluorescence spectroscopy is a simple and reliable methodology to estimate the size of both quantum dot types. For a given solution, the homogeneity of the size of quantum dots is correlated to the relationship between the fluorescence maximum position (FMP) and the quantum dot size. This methodology can be extended to the other fluorescent nanoparticles. The employment of evolving factor analysis and multivariate curve resolution-alternating least squares for decomposition of the series of quantum dots fluorescence spectra recorded by a specific measuring procedure reveals the number of quantum dot fractions having different diameters. The size of the quantum dots in a particular group is defined by the FMP of the corresponding component in the decomposed spectrum. These results show that a combination of the fluorescence and appropriate statistical method for decomposition of the emission spectra of nanoparticles may be a quick and trusted method for the screening of the inhomogeneity of their solution.
PB  - Royal Soc Chemistry, Cambridge
T2  - Analyst
T1  - Determination of the size of quantum dots by fluorescence spectroscopy
EP  - 2396
IS  - 11
SP  - 2391
VL  - 136
DO  - 10.1039/c0an00802h
ER  - 

@article{
author = "Mutavdžić, Dragosav and Xu, Jianmin and Thakur, Garima and Triulzi, Robert and Kasas, Sandor and Jeremic, Milorad G and Leblanc, Roger M. and Radotić, Ksenija",
year = "2011",
abstract = "There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding fluorescence spectrum. It has been found that fluorescence spectroscopy is a simple and reliable methodology to estimate the size of both quantum dot types. For a given solution, the homogeneity of the size of quantum dots is correlated to the relationship between the fluorescence maximum position (FMP) and the quantum dot size. This methodology can be extended to the other fluorescent nanoparticles. The employment of evolving factor analysis and multivariate curve resolution-alternating least squares for decomposition of the series of quantum dots fluorescence spectra recorded by a specific measuring procedure reveals the number of quantum dot fractions having different diameters. The size of the quantum dots in a particular group is defined by the FMP of the corresponding component in the decomposed spectrum. These results show that a combination of the fluorescence and appropriate statistical method for decomposition of the emission spectra of nanoparticles may be a quick and trusted method for the screening of the inhomogeneity of their solution.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Analyst",
title = "Determination of the size of quantum dots by fluorescence spectroscopy",
pages = "2396-2391",
number = "11",
volume = "136",
doi = "10.1039/c0an00802h"
}

Mutavdžić, D., Xu, J., Thakur, G., Triulzi, R., Kasas, S., Jeremic, M. G., Leblanc, R. M.,& Radotić, K.. (2011). Determination of the size of quantum dots by fluorescence spectroscopy. in Analyst
Royal Soc Chemistry, Cambridge., 136(11), 2391-2396.
https://doi.org/10.1039/c0an00802h

Mutavdžić D, Xu J, Thakur G, Triulzi R, Kasas S, Jeremic MG, Leblanc RM, Radotić K. Determination of the size of quantum dots by fluorescence spectroscopy. in Analyst. 2011;136(11):2391-2396.
doi:10.1039/c0an00802h .

Mutavdžić, Dragosav, Xu, Jianmin, Thakur, Garima, Triulzi, Robert, Kasas, Sandor, Jeremic, Milorad G, Leblanc, Roger M., Radotić, Ksenija, "Determination of the size of quantum dots by fluorescence spectroscopy" in Analyst, 136, no. 11 (2011):2391-2396,
https://doi.org/10.1039/c0an00802h . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Đikanović, Daniela
AU  - Jeremic, M
AU  - Leblanc, Roger M.
AU  - Cerović, ZG
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/149
AB  - In order to test whether lignin fluorescence originates from discrete fluorophores, fluorescence emission spectra of the lignin model dehydrogenative polymer (DHP) were analyzed by the band deconvolution method and time-resolved analysis of both the excitation and emission spectra. Two series of 22 fluorescence emission spectra of DHP in chloroform/methanol (3:1, v/v) solution, and as a solid suspension in water, were deconvoluted into three fluorescence and one Raman Gaussian components. Emission spectra were obtained by stepwise variation of the excitation wavelength from 360 to 465 nm. Deconvolution was performed by nonlinear fitting of all three Gaussian parameters: area, width and position. Position of all components in a series was treated as a random variable and its approximate probability distribution (APD) calculated from a series of histograms with increasing number of abscissa intervals. A five peak multimodal APD profile was obtained for both series of DHP emission spectra. The mean fluorescence lifetime varied with wavelength both in the emission and the excitation decay-associated spectra (DAS), where four kinetic components were resolved. The shapes of the excitation spectra of the four components were quite different and gradually shifted bathochromically. The multicomponent nature of the DHP emission spectra along with the changes in the mean fluorescence lifetime and the form of the excitation DAS of the four components give evidence of the heterogeneous origin of fluorescent species emitting in the visible.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Component analysis of the fluorescence spectra of a lignin model compound
EP  - 10
IS  - 1
SP  - 1
VL  - 83
DO  - 10.1016/j.jphotobiol.2005.12.001
ER  - 

@article{
author = "Radotić, Ksenija and Kalauzi, Aleksandar and Đikanović, Daniela and Jeremic, M and Leblanc, Roger M. and Cerović, ZG",
year = "2006",
abstract = "In order to test whether lignin fluorescence originates from discrete fluorophores, fluorescence emission spectra of the lignin model dehydrogenative polymer (DHP) were analyzed by the band deconvolution method and time-resolved analysis of both the excitation and emission spectra. Two series of 22 fluorescence emission spectra of DHP in chloroform/methanol (3:1, v/v) solution, and as a solid suspension in water, were deconvoluted into three fluorescence and one Raman Gaussian components. Emission spectra were obtained by stepwise variation of the excitation wavelength from 360 to 465 nm. Deconvolution was performed by nonlinear fitting of all three Gaussian parameters: area, width and position. Position of all components in a series was treated as a random variable and its approximate probability distribution (APD) calculated from a series of histograms with increasing number of abscissa intervals. A five peak multimodal APD profile was obtained for both series of DHP emission spectra. The mean fluorescence lifetime varied with wavelength both in the emission and the excitation decay-associated spectra (DAS), where four kinetic components were resolved. The shapes of the excitation spectra of the four components were quite different and gradually shifted bathochromically. The multicomponent nature of the DHP emission spectra along with the changes in the mean fluorescence lifetime and the form of the excitation DAS of the four components give evidence of the heterogeneous origin of fluorescent species emitting in the visible.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Component analysis of the fluorescence spectra of a lignin model compound",
pages = "10-1",
number = "1",
volume = "83",
doi = "10.1016/j.jphotobiol.2005.12.001"
}

Radotić, K., Kalauzi, A., Đikanović, D., Jeremic, M., Leblanc, R. M.,& Cerović, Z.. (2006). Component analysis of the fluorescence spectra of a lignin model compound. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 83(1), 1-10.
https://doi.org/10.1016/j.jphotobiol.2005.12.001

Radotić K, Kalauzi A, Đikanović D, Jeremic M, Leblanc RM, Cerović Z. Component analysis of the fluorescence spectra of a lignin model compound. in Journal of Photochemistry and Photobiology B-Biology. 2006;83(1):1-10.
doi:10.1016/j.jphotobiol.2005.12.001 .

Radotić, Ksenija, Kalauzi, Aleksandar, Đikanović, Daniela, Jeremic, M, Leblanc, Roger M., Cerović, ZG, "Component analysis of the fluorescence spectra of a lignin model compound" in Journal of Photochemistry and Photobiology B-Biology, 83, no. 1 (2006):1-10,
https://doi.org/10.1016/j.jphotobiol.2005.12.001 . .

TY  - JOUR
AU  - Micic, M
AU  - Radotić, Ksenija
AU  - Jeremic, M
AU  - Leblanc, Roger M.
PY  - 2003
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/91
AB  - Full Paper: Herein we report a topographical study of the supramolecular organization of enzymatically polymerized lignin model compound - dehydrogenative polymer (DHP) of coniferyl alcohol - on cellulose substrate, using the Environmental Scanning Electron microscope (ESM) technique. A comparison of deposits obtained by bulk polymerization in solution and direct polymerization in the presence of cellulose substrate shows distinct differences in supramolecular organization organization. DHP polymerized in solution expresses a 3-D structure with short range ordered motifs, while the polymer formed in the presence of cellulose substrate arranges in a layer compact structure consisting of several domains. Such structures could be described as a two-dimensional hexagonal close packed lattice (HCPL), with the dimension of unit cell in 1 mum range. The domains have a quite regular structure with few lattice defects, forming a good example of two dimensional colloidal crystal. The growth of DHP globules and their assembly into supramolecular structures are integrated in terms of cooperative electrostatic interaction of polymer precursors, with a framework of cellulose OH groups, and interfacial interactions (hydrophilic/hydrophobic) in the course of the structure growth. The results strongly suggest that the carbohydrate matrix in plant cells can serve as a template for lignin structure formation in the plant cell wall.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Macromolecular Bioscience
T1  - Study of self-assembly of the lignin model compound on cellulose model substrate
EP  - 106
IS  - 2
SP  - 100
VL  - 3
DO  - 10.1002/mabi.200390011
ER  - 

@article{
author = "Micic, M and Radotić, Ksenija and Jeremic, M and Leblanc, Roger M.",
year = "2003",
abstract = "Full Paper: Herein we report a topographical study of the supramolecular organization of enzymatically polymerized lignin model compound - dehydrogenative polymer (DHP) of coniferyl alcohol - on cellulose substrate, using the Environmental Scanning Electron microscope (ESM) technique. A comparison of deposits obtained by bulk polymerization in solution and direct polymerization in the presence of cellulose substrate shows distinct differences in supramolecular organization organization. DHP polymerized in solution expresses a 3-D structure with short range ordered motifs, while the polymer formed in the presence of cellulose substrate arranges in a layer compact structure consisting of several domains. Such structures could be described as a two-dimensional hexagonal close packed lattice (HCPL), with the dimension of unit cell in 1 mum range. The domains have a quite regular structure with few lattice defects, forming a good example of two dimensional colloidal crystal. The growth of DHP globules and their assembly into supramolecular structures are integrated in terms of cooperative electrostatic interaction of polymer precursors, with a framework of cellulose OH groups, and interfacial interactions (hydrophilic/hydrophobic) in the course of the structure growth. The results strongly suggest that the carbohydrate matrix in plant cells can serve as a template for lignin structure formation in the plant cell wall.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Macromolecular Bioscience",
title = "Study of self-assembly of the lignin model compound on cellulose model substrate",
pages = "106-100",
number = "2",
volume = "3",
doi = "10.1002/mabi.200390011"
}

Micic, M., Radotić, K., Jeremic, M.,& Leblanc, R. M.. (2003). Study of self-assembly of the lignin model compound on cellulose model substrate. in Macromolecular Bioscience
Wiley-V C H Verlag Gmbh, Weinheim., 3(2), 100-106.
https://doi.org/10.1002/mabi.200390011

Micic M, Radotić K, Jeremic M, Leblanc RM. Study of self-assembly of the lignin model compound on cellulose model substrate. in Macromolecular Bioscience. 2003;3(2):100-106.
doi:10.1002/mabi.200390011 .

Micic, M, Radotić, Ksenija, Jeremic, M, Leblanc, Roger M., "Study of self-assembly of the lignin model compound on cellulose model substrate" in Macromolecular Bioscience, 3, no. 2 (2003):100-106,
https://doi.org/10.1002/mabi.200390011 . .

TY  - JOUR
AU  - Micic, M
AU  - Orbulescu, J
AU  - Radotić, Ksenija
AU  - Jeremic, M
AU  - Sui, G
AU  - Zheng, Y
AU  - Leblanc, Roger M.
PY  - 2002
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/73
AB  - In this paper we present our surface chemistry studies of enzymatically polymerized, poly-coniferyl alcohol lignin model compound (dehydrogenate polymer a.k.a. ZL-DHP) at the air-water interface. Using the CHCl3/MeOH (5:1 v/v) spreading solvent, we found an average molecular area of ZL-DHP of approximately 1200 Angstrom(2). The monolayer expresses a high compressibility with a collapsed area of 500 Angstrom(2) and collapsed surface pressure of 28 mN m(-1). In the range of applied surface pressures, ZL-DHP polymer have no phase changes, as shown by the very high linearity (R=0.994) of absorbance vs. surface pressure cure. There was no symmetry transitions observed as shown by absence of shifts of absorption peak maximums.
PB  - Elsevier, Amsterdam
T2  - Biophysical Chemistry
T1  - ZL-DHP lignin model compound at the air-water interface
EP  - 62
IS  - 1
SP  - 55
VL  - 99
DO  - 10.1016/S0301-4622(02)00121-7
ER  - 

@article{
author = "Micic, M and Orbulescu, J and Radotić, Ksenija and Jeremic, M and Sui, G and Zheng, Y and Leblanc, Roger M.",
year = "2002",
abstract = "In this paper we present our surface chemistry studies of enzymatically polymerized, poly-coniferyl alcohol lignin model compound (dehydrogenate polymer a.k.a. ZL-DHP) at the air-water interface. Using the CHCl3/MeOH (5:1 v/v) spreading solvent, we found an average molecular area of ZL-DHP of approximately 1200 Angstrom(2). The monolayer expresses a high compressibility with a collapsed area of 500 Angstrom(2) and collapsed surface pressure of 28 mN m(-1). In the range of applied surface pressures, ZL-DHP polymer have no phase changes, as shown by the very high linearity (R=0.994) of absorbance vs. surface pressure cure. There was no symmetry transitions observed as shown by absence of shifts of absorption peak maximums.",
publisher = "Elsevier, Amsterdam",
journal = "Biophysical Chemistry",
title = "ZL-DHP lignin model compound at the air-water interface",
pages = "62-55",
number = "1",
volume = "99",
doi = "10.1016/S0301-4622(02)00121-7"
}

Micic, M., Orbulescu, J., Radotić, K., Jeremic, M., Sui, G., Zheng, Y.,& Leblanc, R. M.. (2002). ZL-DHP lignin model compound at the air-water interface. in Biophysical Chemistry
Elsevier, Amsterdam., 99(1), 55-62.
https://doi.org/10.1016/S0301-4622(02)00121-7

Micic M, Orbulescu J, Radotić K, Jeremic M, Sui G, Zheng Y, Leblanc RM. ZL-DHP lignin model compound at the air-water interface. in Biophysical Chemistry. 2002;99(1):55-62.
doi:10.1016/S0301-4622(02)00121-7 .

Micic, M, Orbulescu, J, Radotić, Ksenija, Jeremic, M, Sui, G, Zheng, Y, Leblanc, Roger M., "ZL-DHP lignin model compound at the air-water interface" in Biophysical Chemistry, 99, no. 1 (2002):55-62,
https://doi.org/10.1016/S0301-4622(02)00121-7 . .

TY  - JOUR
AU  - Micic, M
AU  - Benitez, I
AU  - Ruano, M
AU  - Mavers, M
AU  - Jeremic, M
AU  - Radotić, Ksenija
AU  - Moy, V
AU  - Leblanc, Roger M.
PY  - 2001
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/68
AB  - By combining atomic force microscopy (AFM) force and environmental scanning electron microscopies (ESEMs), herein we present an evidence for the existence of strong intermolecular forces, which are responsible for holding lignin globules together in higher ordered structures. Based on this observation, we provide a support for the hypothesis that lignin globules consist of at least two individual spherical layers, with space in between filled with solvent or gas.
PB  - Elsevier, Amsterdam
T2  - Chemical Physics Letters
T1  - Probing the lignin nanomechanical properties and lignin-lignin interactions using the atomic force microscopy
EP  - 45
IS  - 1-3
SP  - 41
VL  - 347
DO  - 10.1016/S0009-2614(01)01022-3
ER  - 

@article{
author = "Micic, M and Benitez, I and Ruano, M and Mavers, M and Jeremic, M and Radotić, Ksenija and Moy, V and Leblanc, Roger M.",
year = "2001",
abstract = "By combining atomic force microscopy (AFM) force and environmental scanning electron microscopies (ESEMs), herein we present an evidence for the existence of strong intermolecular forces, which are responsible for holding lignin globules together in higher ordered structures. Based on this observation, we provide a support for the hypothesis that lignin globules consist of at least two individual spherical layers, with space in between filled with solvent or gas.",
publisher = "Elsevier, Amsterdam",
journal = "Chemical Physics Letters",
title = "Probing the lignin nanomechanical properties and lignin-lignin interactions using the atomic force microscopy",
pages = "45-41",
number = "1-3",
volume = "347",
doi = "10.1016/S0009-2614(01)01022-3"
}

Micic, M., Benitez, I., Ruano, M., Mavers, M., Jeremic, M., Radotić, K., Moy, V.,& Leblanc, R. M.. (2001). Probing the lignin nanomechanical properties and lignin-lignin interactions using the atomic force microscopy. in Chemical Physics Letters
Elsevier, Amsterdam., 347(1-3), 41-45.
https://doi.org/10.1016/S0009-2614(01)01022-3

Micic M, Benitez I, Ruano M, Mavers M, Jeremic M, Radotić K, Moy V, Leblanc RM. Probing the lignin nanomechanical properties and lignin-lignin interactions using the atomic force microscopy. in Chemical Physics Letters. 2001;347(1-3):41-45.
doi:10.1016/S0009-2614(01)01022-3 .

Micic, M, Benitez, I, Ruano, M, Mavers, M, Jeremic, M, Radotić, Ksenija, Moy, V, Leblanc, Roger M., "Probing the lignin nanomechanical properties and lignin-lignin interactions using the atomic force microscopy" in Chemical Physics Letters, 347, no. 1-3 (2001):41-45,
https://doi.org/10.1016/S0009-2614(01)01022-3 . .

TY  - JOUR
AU  - Micic, M
AU  - Radotić, Ksenija
AU  - Benitez, I
AU  - Ruano, M
AU  - Jeremic, M
AU  - Moy, V
AU  - Mabrouki, M
AU  - Leblanc, Roger M.
PY  - 2001
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/66
AB  - By combining the results from atomic force microscopy (AFM) and environmental scanning electron microscopy (ESEM), herein we investigate properties of photochemical lignin model compounds. We provide evidence that photochemical lignin forms random, probably non-functional structures. The topography of such structures is explored using ESEM. Non-functionality of such structures is proved by AFM and atomic force spectroscopy experiments wherein the photochemical lignin functionalized tip is approached to the substrate covered with photochemical lignin. There was no evidence of existence of any kind of host-guest interaction during the approach/retraction experiments. These results provide evidence for our previously stated hypothesis that photochemical lignin polymerization may be one of the degrading effects of UV radiation to the plant cell.
PB  - Elsevier, Amsterdam
T2  - Biophysical Chemistry
T1  - Topographical characterization and surface force spectroscopy of the photochemical lignin model compound
EP  - 263
IS  - 3
SP  - 257
VL  - 94
DO  - 10.1016/S0301-4622(01)00245-9
ER  - 

@article{
author = "Micic, M and Radotić, Ksenija and Benitez, I and Ruano, M and Jeremic, M and Moy, V and Mabrouki, M and Leblanc, Roger M.",
year = "2001",
abstract = "By combining the results from atomic force microscopy (AFM) and environmental scanning electron microscopy (ESEM), herein we investigate properties of photochemical lignin model compounds. We provide evidence that photochemical lignin forms random, probably non-functional structures. The topography of such structures is explored using ESEM. Non-functionality of such structures is proved by AFM and atomic force spectroscopy experiments wherein the photochemical lignin functionalized tip is approached to the substrate covered with photochemical lignin. There was no evidence of existence of any kind of host-guest interaction during the approach/retraction experiments. These results provide evidence for our previously stated hypothesis that photochemical lignin polymerization may be one of the degrading effects of UV radiation to the plant cell.",
publisher = "Elsevier, Amsterdam",
journal = "Biophysical Chemistry",
title = "Topographical characterization and surface force spectroscopy of the photochemical lignin model compound",
pages = "263-257",
number = "3",
volume = "94",
doi = "10.1016/S0301-4622(01)00245-9"
}

Micic, M., Radotić, K., Benitez, I., Ruano, M., Jeremic, M., Moy, V., Mabrouki, M.,& Leblanc, R. M.. (2001). Topographical characterization and surface force spectroscopy of the photochemical lignin model compound. in Biophysical Chemistry
Elsevier, Amsterdam., 94(3), 257-263.
https://doi.org/10.1016/S0301-4622(01)00245-9

Micic M, Radotić K, Benitez I, Ruano M, Jeremic M, Moy V, Mabrouki M, Leblanc RM. Topographical characterization and surface force spectroscopy of the photochemical lignin model compound. in Biophysical Chemistry. 2001;94(3):257-263.
doi:10.1016/S0301-4622(01)00245-9 .

Micic, M, Radotić, Ksenija, Benitez, I, Ruano, M, Jeremic, M, Moy, V, Mabrouki, M, Leblanc, Roger M., "Topographical characterization and surface force spectroscopy of the photochemical lignin model compound" in Biophysical Chemistry, 94, no. 3 (2001):257-263,
https://doi.org/10.1016/S0301-4622(01)00245-9 . .

TY  - JOUR
AU  - Mičič, M.
AU  - Jeremič, M.
AU  - Radotić, Ksenija
AU  - Mavers, M.
AU  - Leblanc, Roger M.
PY  - 2000
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/54
AB  - In this paper we are presenting the results of our environmental scanning electron microscopy (ESEM) investigation of the lignin model compound-enzymatically polymerized coniferyl alcohol, also known as dehydrogenate polymer (DHP). The goals of this study were to visualize the supramolecular organization of DHP polymer on various substrates, namely graphite, mica, and glass, and to explore the influence of substrate surface properties and associated collective phenomena on the lignin self-assembled supramolecular structure. Based on results obtained with ESEM, combined with previously published results based on scanning tunneling microscopy (STM) and electron spin resonance (ESR) technique, we looked at Lignin structure ranging from a monomer on a fraction of nanometer scale to a large aggregate on a fraction of millimeter scale. therefore using six orders of magnitude range of size. Herein, we are presenting evidence that there are at least four different levels of the supramolecular structure of lignin, and that its supramolecular organization is well dependent on the substrate surface characteristics, such as hydrophobicity, delocalized orbitals, and surface-free entry.
PB  - Hindawi-Wiley
T2  - Scanning
T1  - Visualization of artificial lignin supramolecular structures
EP  - 294
IS  - 5
SP  - 288
VL  - 22
DO  - 10.1002/sca.4950220503
ER  - 

@article{
author = "Mičič, M. and Jeremič, M. and Radotić, Ksenija and Mavers, M. and Leblanc, Roger M.",
year = "2000",
abstract = "In this paper we are presenting the results of our environmental scanning electron microscopy (ESEM) investigation of the lignin model compound-enzymatically polymerized coniferyl alcohol, also known as dehydrogenate polymer (DHP). The goals of this study were to visualize the supramolecular organization of DHP polymer on various substrates, namely graphite, mica, and glass, and to explore the influence of substrate surface properties and associated collective phenomena on the lignin self-assembled supramolecular structure. Based on results obtained with ESEM, combined with previously published results based on scanning tunneling microscopy (STM) and electron spin resonance (ESR) technique, we looked at Lignin structure ranging from a monomer on a fraction of nanometer scale to a large aggregate on a fraction of millimeter scale. therefore using six orders of magnitude range of size. Herein, we are presenting evidence that there are at least four different levels of the supramolecular structure of lignin, and that its supramolecular organization is well dependent on the substrate surface characteristics, such as hydrophobicity, delocalized orbitals, and surface-free entry.",
publisher = "Hindawi-Wiley",
journal = "Scanning",
title = "Visualization of artificial lignin supramolecular structures",
pages = "294-288",
number = "5",
volume = "22",
doi = "10.1002/sca.4950220503"
}

Mičič, M., Jeremič, M., Radotić, K., Mavers, M.,& Leblanc, R. M.. (2000). Visualization of artificial lignin supramolecular structures. in Scanning
Hindawi-Wiley., 22(5), 288-294.
https://doi.org/10.1002/sca.4950220503

Mičič M, Jeremič M, Radotić K, Mavers M, Leblanc RM. Visualization of artificial lignin supramolecular structures. in Scanning. 2000;22(5):288-294.
doi:10.1002/sca.4950220503 .

Mičič, M., Jeremič, M., Radotić, Ksenija, Mavers, M., Leblanc, Roger M., "Visualization of artificial lignin supramolecular structures" in Scanning, 22, no. 5 (2000):288-294,
https://doi.org/10.1002/sca.4950220503 . .

TY  - JOUR
AU  - Micic, M
AU  - Jeremic, M
AU  - Radotić, Ksenija
AU  - Leblanc, Roger M.
PY  - 2000
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/50
AB  - Environmental scanning electron microscopy images of the self-assembled structures of enzymatically (DHP) and photochemically polymerized (PCP) artificial lignin are herein presented. Differences in the structural organization between DHP and PCP polymer at the supramolecular level were reported. Based on topological information, we proposed a hypothesis about possible new physiological roles of lignin in live plant cells and the ecological significance of possible in-vivo photochemical lignin polymerization.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Colloid and Interface Science
T1  - A comparative study of enzymatically and photochemically polymerized artificial lignin supramolecular structures using environmental scanning electron microscopy
EP  - 194
IS  - 1
SP  - 190
VL  - 231
DO  - 10.1006/jcis.2000.7136
ER  - 

@article{
author = "Micic, M and Jeremic, M and Radotić, Ksenija and Leblanc, Roger M.",
year = "2000",
abstract = "Environmental scanning electron microscopy images of the self-assembled structures of enzymatically (DHP) and photochemically polymerized (PCP) artificial lignin are herein presented. Differences in the structural organization between DHP and PCP polymer at the supramolecular level were reported. Based on topological information, we proposed a hypothesis about possible new physiological roles of lignin in live plant cells and the ecological significance of possible in-vivo photochemical lignin polymerization.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Colloid and Interface Science",
title = "A comparative study of enzymatically and photochemically polymerized artificial lignin supramolecular structures using environmental scanning electron microscopy",
pages = "194-190",
number = "1",
volume = "231",
doi = "10.1006/jcis.2000.7136"
}

Micic, M., Jeremic, M., Radotić, K.,& Leblanc, R. M.. (2000). A comparative study of enzymatically and photochemically polymerized artificial lignin supramolecular structures using environmental scanning electron microscopy. in Journal of Colloid and Interface Science
Academic Press Inc Elsevier Science, San Diego., 231(1), 190-194.
https://doi.org/10.1006/jcis.2000.7136

Micic M, Jeremic M, Radotić K, Leblanc RM. A comparative study of enzymatically and photochemically polymerized artificial lignin supramolecular structures using environmental scanning electron microscopy. in Journal of Colloid and Interface Science. 2000;231(1):190-194.
doi:10.1006/jcis.2000.7136 .

Micic, M, Jeremic, M, Radotić, Ksenija, Leblanc, Roger M., "A comparative study of enzymatically and photochemically polymerized artificial lignin supramolecular structures using environmental scanning electron microscopy" in Journal of Colloid and Interface Science, 231, no. 1 (2000):190-194,
https://doi.org/10.1006/jcis.2000.7136 . .