TY  - JOUR
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Jeremic, Milorad G
AU  - Xu, Jianmin
AU  - Micic, Miodrag
AU  - Whyte, Jeffrey D
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/521
AB  - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of the CdSe quantum dots with plant cell walls
EP  - 47
SP  - 41
VL  - 91
DO  - 10.1016/j.colsurfb.2011.10.032
ER  - 

@article{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Jeremic, Milorad G and Xu, Jianmin and Micic, Miodrag and Whyte, Jeffrey D and Leblanc, Roger M. and Radotić, Ksenija",
year = "2012",
abstract = "There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of the CdSe quantum dots with plant cell walls",
pages = "47-41",
volume = "91",
doi = "10.1016/j.colsurfb.2011.10.032"
}

Đikanović, D., Kalauzi, A., Jeremic, M. G., Xu, J., Micic, M., Whyte, J. D., Leblanc, R. M.,& Radotić, K.. (2012). Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 91, 41-47.
https://doi.org/10.1016/j.colsurfb.2011.10.032

Đikanović D, Kalauzi A, Jeremic MG, Xu J, Micic M, Whyte JD, Leblanc RM, Radotić K. Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces. 2012;91:41-47.
doi:10.1016/j.colsurfb.2011.10.032 .

Đikanović, Daniela, Kalauzi, Aleksandar, Jeremic, Milorad G, Xu, Jianmin, Micic, Miodrag, Whyte, Jeffrey D, Leblanc, Roger M., Radotić, Ksenija, "Interaction of the CdSe quantum dots with plant cell walls" in Colloids and Surfaces B-Biointerfaces, 91 (2012):41-47,
https://doi.org/10.1016/j.colsurfb.2011.10.032 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Jeremic, Milorad
AU  - Micic, Miodrag
AU  - Radotić, Ksenija
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/190
AB  - Fluorescence spectroscopy is a sensitive analytical tool in the studies of both simple and complex molecular structures. In complex molecules, however, determining the number and position of components may give a specific insight into the structure, complementary to the other analytical techniques. We applied log-normal model to analyze fluorescence of simple monofluorophore molecule. In order to analyze spectra where both fluorophores and Raman emission bands were present, we developed a method obtained by combination of the symmetric, Gaussian, for Raman and asymmetric, log-normal model, for fluorescence, applicable to the molecules of different complexity. Technically, for each sample we varied excitation wavelength with 5 nm step and recorded the corresponding emission spectra. They were subsequently used for component analysis. Position of each component was plotted against the excitation wavelength, Applying this approach we could identify minimal number of components having stable positions, while their approximate probability density (APD) in a spectral series was correlated with the probable number of fluorophores in the molecule. The method was tested on molecules containing different number of fluorophores: monomers involved in the synthesis of plant polymer lignin-coniferyl alcohol (one fluorophore), ferulic acid (two fluorophores) and on lignin model compound produced from these monomers (many fluorophores). All investigated species belong to benzene-substituted class of compounds, and it is reasonable to assume that they have similar fluorescence band contour. We also report the results of environmental scanning electron microscopy (ESEM) studies showing multilayered dehydrogenative polymer (DHP) structure, in order to show complexity of the polymer. Our results present complementarity of these two approaches in the structural studies of the lignin model compound.
PB  - Elsevier Science Bv, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Deconvolution of fluorescence spectra: Contribution to the structural analysis of complex molecules
EP  - 192
IS  - 2
SP  - 188
VL  - 54
DO  - 10.1016/j.colsurfb.2006.10.015
ER  - 

@article{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Jeremic, Milorad and Micic, Miodrag and Radotić, Ksenija",
year = "2007",
abstract = "Fluorescence spectroscopy is a sensitive analytical tool in the studies of both simple and complex molecular structures. In complex molecules, however, determining the number and position of components may give a specific insight into the structure, complementary to the other analytical techniques. We applied log-normal model to analyze fluorescence of simple monofluorophore molecule. In order to analyze spectra where both fluorophores and Raman emission bands were present, we developed a method obtained by combination of the symmetric, Gaussian, for Raman and asymmetric, log-normal model, for fluorescence, applicable to the molecules of different complexity. Technically, for each sample we varied excitation wavelength with 5 nm step and recorded the corresponding emission spectra. They were subsequently used for component analysis. Position of each component was plotted against the excitation wavelength, Applying this approach we could identify minimal number of components having stable positions, while their approximate probability density (APD) in a spectral series was correlated with the probable number of fluorophores in the molecule. The method was tested on molecules containing different number of fluorophores: monomers involved in the synthesis of plant polymer lignin-coniferyl alcohol (one fluorophore), ferulic acid (two fluorophores) and on lignin model compound produced from these monomers (many fluorophores). All investigated species belong to benzene-substituted class of compounds, and it is reasonable to assume that they have similar fluorescence band contour. We also report the results of environmental scanning electron microscopy (ESEM) studies showing multilayered dehydrogenative polymer (DHP) structure, in order to show complexity of the polymer. Our results present complementarity of these two approaches in the structural studies of the lignin model compound.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Deconvolution of fluorescence spectra: Contribution to the structural analysis of complex molecules",
pages = "192-188",
number = "2",
volume = "54",
doi = "10.1016/j.colsurfb.2006.10.015"
}

Đikanović, D., Kalauzi, A., Jeremic, M., Micic, M.,& Radotić, K.. (2007). Deconvolution of fluorescence spectra: Contribution to the structural analysis of complex molecules. in Colloids and Surfaces B-Biointerfaces
Elsevier Science Bv, Amsterdam., 54(2), 188-192.
https://doi.org/10.1016/j.colsurfb.2006.10.015

Đikanović D, Kalauzi A, Jeremic M, Micic M, Radotić K. Deconvolution of fluorescence spectra: Contribution to the structural analysis of complex molecules. in Colloids and Surfaces B-Biointerfaces. 2007;54(2):188-192.
doi:10.1016/j.colsurfb.2006.10.015 .

Đikanović, Daniela, Kalauzi, Aleksandar, Jeremic, Milorad, Micic, Miodrag, Radotić, Ksenija, "Deconvolution of fluorescence spectra: Contribution to the structural analysis of complex molecules" in Colloids and Surfaces B-Biointerfaces, 54, no. 2 (2007):188-192,
https://doi.org/10.1016/j.colsurfb.2006.10.015 . .