TY  - JOUR
AU  - Radotić, Ksenija
AU  - Melo, Thor Bernt
AU  - Leblanc, Roger M.
AU  - Yousef, Yaser A.
AU  - Naqvi, K. Razi
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/988
AB  - To interpret accurately protein fluorescence and phosphorescence, it is essential to achieve a better understanding of the luminescence properties of tryptophan (Trp, or W) in peptides. In published literature data on luminescence of peptides of varied length are scarce. This article describes studies of fluorescence and phosphorescence properties of the eight Trp-containing synthetic peptides: WAK, AWK, SWA, KYLWE, AVSWK, WVSWAK, WAKLAWE, and AVSWAKLARE. The aim was to investigate which factors influence the fluorescence yield and phosphorescence-spectra and lifetimes. Absorption spectra, room temperature fluorescence emission and corresponding excitation spectra and time-resolved phosphorescence spectra (77 K) have been recorded; the dependence of the fluorescence quantum yield on the specific peptide and its variation with the wavelength of excitation has been studied. The changes in fluorescence yield and shape of phosphorescence spectra are explained in terms of intemal electron and proton transfer. The structured phosphorescence spectrum originates from proton transfer occurring upon excitation of Trp, while electron transfer gives rise to a non-structured luminescence spectrum. There is also electron transfer from higher vibronic S-1 states. In the peptides there is higher probability of electron transfer than in Trp alone. The obtained data are interpreted in light of the peptides' sequence, length and conformation.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence
EP  - 128
SP  - 120
VL  - 157
DO  - 10.1016/j.jphotobiol.2016.02.011
ER  - 

@article{
author = "Radotić, Ksenija and Melo, Thor Bernt and Leblanc, Roger M. and Yousef, Yaser A. and Naqvi, K. Razi",
year = "2016",
abstract = "To interpret accurately protein fluorescence and phosphorescence, it is essential to achieve a better understanding of the luminescence properties of tryptophan (Trp, or W) in peptides. In published literature data on luminescence of peptides of varied length are scarce. This article describes studies of fluorescence and phosphorescence properties of the eight Trp-containing synthetic peptides: WAK, AWK, SWA, KYLWE, AVSWK, WVSWAK, WAKLAWE, and AVSWAKLARE. The aim was to investigate which factors influence the fluorescence yield and phosphorescence-spectra and lifetimes. Absorption spectra, room temperature fluorescence emission and corresponding excitation spectra and time-resolved phosphorescence spectra (77 K) have been recorded; the dependence of the fluorescence quantum yield on the specific peptide and its variation with the wavelength of excitation has been studied. The changes in fluorescence yield and shape of phosphorescence spectra are explained in terms of intemal electron and proton transfer. The structured phosphorescence spectrum originates from proton transfer occurring upon excitation of Trp, while electron transfer gives rise to a non-structured luminescence spectrum. There is also electron transfer from higher vibronic S-1 states. In the peptides there is higher probability of electron transfer than in Trp alone. The obtained data are interpreted in light of the peptides' sequence, length and conformation.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence",
pages = "128-120",
volume = "157",
doi = "10.1016/j.jphotobiol.2016.02.011"
}

Radotić, K., Melo, T. B., Leblanc, R. M., Yousef, Y. A.,& Naqvi, K. R.. (2016). Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 157, 120-128.
https://doi.org/10.1016/j.jphotobiol.2016.02.011

Radotić K, Melo TB, Leblanc RM, Yousef YA, Naqvi KR. Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. in Journal of Photochemistry and Photobiology B-Biology. 2016;157:120-128.
doi:10.1016/j.jphotobiol.2016.02.011 .

Radotić, Ksenija, Melo, Thor Bernt, Leblanc, Roger M., Yousef, Yaser A., Naqvi, K. Razi, "Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence" in Journal of Photochemistry and Photobiology B-Biology, 157 (2016):120-128,
https://doi.org/10.1016/j.jphotobiol.2016.02.011 . .