TY  - CONF
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Sedlarević Zorić, Ana
AU  - Jelušić, Aleksandra
AU  - Šušić, Nikola
AU  - Marković, Sanja
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2058
AB  - Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth & Environmental Research
T1  - Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season
EP  - 134
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2058
ER  - 

@conference{
author = "Živanović, Bojana and Milić Komić, Sonja and Sedlarević Zorić, Ana and Jelušić, Aleksandra and Šušić, Nikola and Marković, Sanja and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "Due to increased urbanization and industrialization, the emission of toxic material into the
atmosphere is in expansion, which has a negative impact on the environment and human health. In
this research, we monitored the effect of air pollution on the peroxidase (POD) activity and total
antioxidant capacity of different tree species during the growing season. The main goal was to
determine which tree species developed the highest tolerance to unfavorable environmental
conditions at the end of growing season, based on the response of their antioxidative metabolism. The
greatest change in POD activity was observed in the Fagus sylvatica L. leaves, where enzyme activity
was more than doubled in the autumn, in comparison to spring. On the other hand, decrease in POD
activity was the greatest in Cedrus atlantica (Endl.) Manetti ex Carrière needles. Moreover, total
antioxidative capacity was altered during growing season in almost all examined tree species. The
Magnolia spp. showed the most consistent response to the given environmental pollution with both
portrayed parameters induced during growing season. In general, we can conclude that the tree
species investigated in this research possess distinctive tolerance potential to air pollutants.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth & Environmental Research",
title = "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season",
pages = "134-129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2058"
}

Živanović, B., Milić Komić, S., Sedlarević Zorić, A., Jelušić, A., Šušić, N., Marković, S.,& Veljović-Jovanović, S.. (2023). Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research
University of Belgrade, Technical Faculty in Bor., 129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058

Živanović B, Milić Komić S, Sedlarević Zorić A, Jelušić A, Šušić N, Marković S, Veljović-Jovanović S. Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season. in 30th International Conference Ecological Truth & Environmental Research. 2023;:129-134.
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .

Živanović, Bojana, Milić Komić, Sonja, Sedlarević Zorić, Ana, Jelušić, Aleksandra, Šušić, Nikola, Marković, Sanja, Veljović-Jovanović, Sonja, "Use of biochemical methods for assessing oxidative stress in trees in urban area during growing season" in 30th International Conference Ecological Truth & Environmental Research (2023):129-134,
https://hdl.handle.net/21.15107/rcub_rimsi_2058 .

TY  - CONF
AU  - Šušić, Nikola
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Sedlarević Zorić, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1984
AB  - The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
T1  - ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION
EP  - 140
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1984
ER  - 

@conference{
author = "Šušić, Nikola and Milić Komić, Sonja and Živanović, Bojana and Jelušić, Aleksandra and Marković, Sanja and Sedlarević Zorić, Ana and Veljović-Jovanović, Sonja",
year = "2023",
abstract = "The first months are the most sensitive phase of oak seedling development. Light conditions have an important role in this sense, both from the physiological and management viewpoint. We investigated the response of pedunculate oak seedlings to three growth light intensities (100, 550 and 2000 μmol cm−2s−1) during development of the first and second growth flush. The low and high light intensities of PAR are supposed to mimic the effects of solar radiation under extreme natural conditions (closed canopy and open field). The response of seedlings to different light intensities was evaluated by determining the photochemical activity of photosystem II, leaf chlorophyll concentration and epidermal flavonoid accumulation for both growth flushes. At the end of the experiment (after 4.5 months) the effects of different treatments on growth parameters were also determined. We showed here that oak seedlings responded to varying light intensities by modifying their physiological and morphological traits of successive growth flushes. At medium light, seedlings had the highest PSII photochemical activity in the 2nd flush. High light induced very low photochemical efficiency of photosystem II in both growth flushes indicating the development of high non-photochemical quenching of chlorophyll fluorescence as part of photoprotective mechanism. In accordance with high photosynthetic yield at ML, the investment of photosynthates in growth, especially biomass allocation towards root system was confirmed. ML was optimal for seedling development in the first months. The results may contribute to a better understanding of oak seedling development and acclimation and could have importance for oak natural regeneration.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23",
title = "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION",
pages = "140-135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1984"
}

Šušić, N., Milić Komić, S., Živanović, B., Jelušić, A., Marković, S., Sedlarević Zorić, A.,& Veljović-Jovanović, S.. (2023). ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984

Šušić N, Milić Komić S, Živanović B, Jelušić A, Marković S, Sedlarević Zorić A, Veljović-Jovanović S. ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION. in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23. 2023;:135-140.
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .

Šušić, Nikola, Milić Komić, Sonja, Živanović, Bojana, Jelušić, Aleksandra, Marković, Sanja, Sedlarević Zorić, Ana, Veljović-Jovanović, Sonja, "ACCLIMATION OF PEDUNCULATE OAK SEEDLINGS TO DIFFERENT LIGHT CONDITIONS IN THE FIRST MONTHS AFTER GERMINATION" in 30th INTERNATIONAL CONFERENCE ECOLOGICAL TRUTH AND ENVIRONMENTAL RESEARCH – EcoTER’23 (2023):135-140,
https://hdl.handle.net/21.15107/rcub_rimsi_1984 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Medić, Olja
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2116
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier Inc.
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
EP  - 13
SP  - 105238
SP  - 1
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Medić, Olja and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists’ potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier Inc.",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "13-105238-1",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Iličić, R., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier Inc.., 182, 105238-13.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Iličić R, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238-13.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Medić, Olja, Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238-13,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2117
AB  - The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic
origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1919
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible
for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo
F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of
cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were
observed during August 2021, while the inner tissues were macerated, featuring cream to black
discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence
ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP)
medium were characterized for their phenotypic and genotypic features. The pathogenicity was
confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase
chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as
well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus
sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were
identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as
Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating
the presence of diverse Pectobacterium species even in combined infection in the same field. Among
the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and
P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus
sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA,
dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the
phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was
found to be the most virulent species for cabbage, followed by P. versatile, while all three species
had comparable virulence with respect to potato. The results obtained in this work provide a better
understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - THES
AU  - Marković, Sanja
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1962
AB  - U Srbiji krompir ima veliki privredni značaj i pripada vodećim ratarsko-povrtarskim kulturama. 
Tokom monitoringa sprovedenog od 2015 do 2019. godine, obuhvaćeno je preko 2000 ha useva 
krompira sa 16 lokaliteta. Rezultati monitoringa su ukazali na prisustvo dve bakteriozne bolesti. Mrka
trulež krtola krompira utvrđena je u 2015, 2016. i 2018. godini i kao uzročnik je identifikovana
karantinska bakterija Ralstonia solanacearum. „Crna noga“ i vlažna trulež krtola krompira, koju su 
prouzrokovale bakterije Pectobacterium carotovorum, Pectobacterium brasiliense i Dickeya 
dianthicola, detektovana je u 2016, 2018. i 2019. godini. U ovom istraživanju, prisustvo P. brasiliense
i D. dianthicola prvi put je zabeleženo na krompiru u Srbiji. Izolati R. solanacearum dobijeni iz 229 
uzoraka sa mrkom truleži krtola bili su fenotipski homogeni, a na osnovu molekularno-genetičke
karakterizacije svrstani su u filotip II. Rezultati ispitivanja fenotipskih karakteristika izolata iz 50
uzoraka sa crnom nogom i vlažnom truleži krtola ukazali su na postojanje uzročnika iz dva roda, 
Pectobacterium i Dickeya. Međutim, molekularno-genetička karakterizacija ovih izolata ukazala je 
na prisustvo tri vrste, pri čemu su P. brasiliense izolati bili heterogeni, dok su P. carotovorum i D. 
dianthicola izolati uglavnom bili homogeni. Metabarkoding analiza omogućila je uvid u sastav 
bakterijske mikrobijalne zajednice krtola krompira sa i bez simptoma vlažne truleži, kao i 
odgovarajućih zemljišta ukazujući da razvoj vlažne truleži nastaje zbog složenih interakcija između 
biljnih patogena i drugih endofitnih bakterija. Biokontrolna aktivnost Bacillus amyloliquefaciens
sojeva SS-12.6 i SS-38.4 prema izolovanim fitopatogenim bakterijama je dokazana u in vitro, in situ 
i in planta uslovima.
T2  - UNIVERZITET U BEOGRADU BIOLOŠKI FAKULTET
T1  - MONITORING, IDENTIFIKACIJA I  MOLEKULARNO-GENETIČKA  KARAKTERIZACIJA FITOPATOGENIH  BAKTERIJA KROMPIRA (SOLANUM  TUBEROSUM L.)
EP  - 141
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1962
ER  - 

@phdthesis{
author = "Marković, Sanja",
year = "2023",
abstract = "U Srbiji krompir ima veliki privredni značaj i pripada vodećim ratarsko-povrtarskim kulturama. 
Tokom monitoringa sprovedenog od 2015 do 2019. godine, obuhvaćeno je preko 2000 ha useva 
krompira sa 16 lokaliteta. Rezultati monitoringa su ukazali na prisustvo dve bakteriozne bolesti. Mrka
trulež krtola krompira utvrđena je u 2015, 2016. i 2018. godini i kao uzročnik je identifikovana
karantinska bakterija Ralstonia solanacearum. „Crna noga“ i vlažna trulež krtola krompira, koju su 
prouzrokovale bakterije Pectobacterium carotovorum, Pectobacterium brasiliense i Dickeya 
dianthicola, detektovana je u 2016, 2018. i 2019. godini. U ovom istraživanju, prisustvo P. brasiliense
i D. dianthicola prvi put je zabeleženo na krompiru u Srbiji. Izolati R. solanacearum dobijeni iz 229 
uzoraka sa mrkom truleži krtola bili su fenotipski homogeni, a na osnovu molekularno-genetičke
karakterizacije svrstani su u filotip II. Rezultati ispitivanja fenotipskih karakteristika izolata iz 50
uzoraka sa crnom nogom i vlažnom truleži krtola ukazali su na postojanje uzročnika iz dva roda, 
Pectobacterium i Dickeya. Međutim, molekularno-genetička karakterizacija ovih izolata ukazala je 
na prisustvo tri vrste, pri čemu su P. brasiliense izolati bili heterogeni, dok su P. carotovorum i D. 
dianthicola izolati uglavnom bili homogeni. Metabarkoding analiza omogućila je uvid u sastav 
bakterijske mikrobijalne zajednice krtola krompira sa i bez simptoma vlažne truleži, kao i 
odgovarajućih zemljišta ukazujući da razvoj vlažne truleži nastaje zbog složenih interakcija između 
biljnih patogena i drugih endofitnih bakterija. Biokontrolna aktivnost Bacillus amyloliquefaciens
sojeva SS-12.6 i SS-38.4 prema izolovanim fitopatogenim bakterijama je dokazana u in vitro, in situ 
i in planta uslovima.",
journal = "UNIVERZITET U BEOGRADU BIOLOŠKI FAKULTET",
title = "MONITORING, IDENTIFIKACIJA I  MOLEKULARNO-GENETIČKA  KARAKTERIZACIJA FITOPATOGENIH  BAKTERIJA KROMPIRA (SOLANUM  TUBEROSUM L.)",
pages = "141-1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1962"
}

Marković, S.. (2023). MONITORING, IDENTIFIKACIJA I  MOLEKULARNO-GENETIČKA  KARAKTERIZACIJA FITOPATOGENIH  BAKTERIJA KROMPIRA (SOLANUM  TUBEROSUM L.). in UNIVERZITET U BEOGRADU BIOLOŠKI FAKULTET, 1-141.
https://hdl.handle.net/21.15107/rcub_rimsi_1962

Marković S. MONITORING, IDENTIFIKACIJA I  MOLEKULARNO-GENETIČKA  KARAKTERIZACIJA FITOPATOGENIH  BAKTERIJA KROMPIRA (SOLANUM  TUBEROSUM L.). in UNIVERZITET U BEOGRADU BIOLOŠKI FAKULTET. 2023;:1-141.
https://hdl.handle.net/21.15107/rcub_rimsi_1962 .

Marković, Sanja, "MONITORING, IDENTIFIKACIJA I  MOLEKULARNO-GENETIČKA  KARAKTERIZACIJA FITOPATOGENIH  BAKTERIJA KROMPIRA (SOLANUM  TUBEROSUM L.)" in UNIVERZITET U BEOGRADU BIOLOŠKI FAKULTET (2023):1-141,
https://hdl.handle.net/21.15107/rcub_rimsi_1962 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1946
AB  - Biokontrola, kao mera zaštite bilja, predstavlja primenu mikroorganizama koji 
ispoljavaju antagonističko delovanje prema patogenom organizmu. Dejstvo mikrobnih 
biokontrolnih agenasa može biti direktno (produkcijom sekundarnih metabolita) ili 
indirektno (kompeticijom za prostor i nutrijente ili indukcijom sistemske rezistencije kod 
biljaka). Bakterije roda Xanthomonas predstavljaju značajnu grupu patogena, koji kod brojnih 
domaćina prouzrokuju tipove simptoma bakteriozne pegavosti, plamenjače, crne truleži i 
bakterioznog raka. Cilj ovog rada je bio da se u in vitro uslovima ispita antagonistički 
potencijal kolekcije izolata iz rodova Bacillus i Pseudomonas prema različitim patogenima 
Xanthomonas spp. 
Potencijalni antagonistički izolati korišćeni u radu su dobijeni iz različitih prirodnih 
staništa, od kojih je na osnovu morfologije kolonija na hranljivom agaru i sekvenci gena za 16S 
rRNK (P0/P6 prajmeri), devet izolata identifikovano kao Bacillus sp. (B33, BA22, B44, B52, 
B62, B64, B114, B122, BS3), a sedam kao Pseudomonas sp. (P7, P9, P10-21, P64, P-1P, P21P, 
P2/2). Antagonistička aktivnost je ispitana prema devet Xanthomonas sojeva, i to: X. 
campestris pv. campestris (patotip soj NCPPB528 i sojevi poreklom sa uljane repice 
NCPPB4679 i XcL1222), X. arboricola pv. pruni (NCPPB3156), X. arboricola pv. juglandis (Xj1),
X. arboricola pv. corylina (XH2), X. euvesicatoria (X22), X. axonopodis pv. phaseoli var. fuscans
(CFBP6165) i X. hortorum pv. pelargonii (NCPPB3330). In vitro antagonistička aktivnost je 
ispitana primenom metode dvojne kulture. U sterilisanu i delimično prohlađenu podlogu od 
hranljivog agara dodata je suspenzija fitopatogena (108 ćel mL-1). Nakon sušenja podloge, na 
površinu su bakteriološkom petljom tačkasto nanošeni biokontrolni sojevi. Ogled je 
postavljen dva puta nezavisno, u četiri ponavljanja. Ocena ogleda vršena je nakon 48 sati, 
merenjem prečnika zone inhibicije (mm). Rezultati su statistički obrađeni u programu 
STATISTICA v. 8 (StatSoft, Inc.), a srednje vrednosti izračunate korišćnjem Tukey’s HSD testa. 
Dobijeni rezultati su smatrani statistički značajnim za vrednosti p < 0,05. 
Rezultati su pokazali da je šest izolata, pet Bacillus spp. (BA22, B64, BS3, B52, B62) i 
jedan Pseudomonas spp. (P7), pokazalo inhibitornu aktivnost prema svim testiranim 
Xanthomonas sojevima. Najveću zonu inhibicije ispoljili su izolati P7 prema soju XH2 (14,67 
mm) i B33 prema soju CFBP6165 (11,67 mm). Izolati P10-21 i P9 su ispoljili antagonizam 
samo u slučaju sojeva X22 (P10-21) i NCPPB528 i XcL1222 (P9), dok izolat P64 ni u jednom 
slučaju nije pokazao inhibitornu aktivnost. 
Dalja istraživanja će obuhvatiti in vivo oglede i identifikaciju biokontrolnih izolata sa 
najizraženijom aktivnošću sekvenciranjem gena za gyrB i tuf.
PB  - DRUŠTVO ZA ZAŠTITU BILJA SRBIJE
C3  - XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
T1  - BIOKONTROLNI POTENCIJAL NEKIH VRSTA BACILLUS I PSEUDOMONAS PREMA  PATOGENIMA IZ RODA XANTHOMONAS
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1946
ER  - 

@conference{
author = "Jelušić, Aleksandra and Marković, Sanja and Iličić, Renata and Popović Milovanović, Tatjana",
year = "2022",
abstract = "Biokontrola, kao mera zaštite bilja, predstavlja primenu mikroorganizama koji 
ispoljavaju antagonističko delovanje prema patogenom organizmu. Dejstvo mikrobnih 
biokontrolnih agenasa može biti direktno (produkcijom sekundarnih metabolita) ili 
indirektno (kompeticijom za prostor i nutrijente ili indukcijom sistemske rezistencije kod 
biljaka). Bakterije roda Xanthomonas predstavljaju značajnu grupu patogena, koji kod brojnih 
domaćina prouzrokuju tipove simptoma bakteriozne pegavosti, plamenjače, crne truleži i 
bakterioznog raka. Cilj ovog rada je bio da se u in vitro uslovima ispita antagonistički 
potencijal kolekcije izolata iz rodova Bacillus i Pseudomonas prema različitim patogenima 
Xanthomonas spp. 
Potencijalni antagonistički izolati korišćeni u radu su dobijeni iz različitih prirodnih 
staništa, od kojih je na osnovu morfologije kolonija na hranljivom agaru i sekvenci gena za 16S 
rRNK (P0/P6 prajmeri), devet izolata identifikovano kao Bacillus sp. (B33, BA22, B44, B52, 
B62, B64, B114, B122, BS3), a sedam kao Pseudomonas sp. (P7, P9, P10-21, P64, P-1P, P21P, 
P2/2). Antagonistička aktivnost je ispitana prema devet Xanthomonas sojeva, i to: X. 
campestris pv. campestris (patotip soj NCPPB528 i sojevi poreklom sa uljane repice 
NCPPB4679 i XcL1222), X. arboricola pv. pruni (NCPPB3156), X. arboricola pv. juglandis (Xj1),
X. arboricola pv. corylina (XH2), X. euvesicatoria (X22), X. axonopodis pv. phaseoli var. fuscans
(CFBP6165) i X. hortorum pv. pelargonii (NCPPB3330). In vitro antagonistička aktivnost je 
ispitana primenom metode dvojne kulture. U sterilisanu i delimično prohlađenu podlogu od 
hranljivog agara dodata je suspenzija fitopatogena (108 ćel mL-1). Nakon sušenja podloge, na 
površinu su bakteriološkom petljom tačkasto nanošeni biokontrolni sojevi. Ogled je 
postavljen dva puta nezavisno, u četiri ponavljanja. Ocena ogleda vršena je nakon 48 sati, 
merenjem prečnika zone inhibicije (mm). Rezultati su statistički obrađeni u programu 
STATISTICA v. 8 (StatSoft, Inc.), a srednje vrednosti izračunate korišćnjem Tukey’s HSD testa. 
Dobijeni rezultati su smatrani statistički značajnim za vrednosti p < 0,05. 
Rezultati su pokazali da je šest izolata, pet Bacillus spp. (BA22, B64, BS3, B52, B62) i 
jedan Pseudomonas spp. (P7), pokazalo inhibitornu aktivnost prema svim testiranim 
Xanthomonas sojevima. Najveću zonu inhibicije ispoljili su izolati P7 prema soju XH2 (14,67 
mm) i B33 prema soju CFBP6165 (11,67 mm). Izolati P10-21 i P9 su ispoljili antagonizam 
samo u slučaju sojeva X22 (P10-21) i NCPPB528 i XcL1222 (P9), dok izolat P64 ni u jednom 
slučaju nije pokazao inhibitornu aktivnost. 
Dalja istraživanja će obuhvatiti in vivo oglede i identifikaciju biokontrolnih izolata sa 
najizraženijom aktivnošću sekvenciranjem gena za gyrB i tuf.",
publisher = "DRUŠTVO ZA ZAŠTITU BILJA SRBIJE",
journal = "XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova",
title = "BIOKONTROLNI POTENCIJAL NEKIH VRSTA BACILLUS I PSEUDOMONAS PREMA  PATOGENIMA IZ RODA XANTHOMONAS",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1946"
}

Jelušić, A., Marković, S., Iličić, R.,& Popović Milovanović, T.. (2022). BIOKONTROLNI POTENCIJAL NEKIH VRSTA BACILLUS I PSEUDOMONAS PREMA  PATOGENIMA IZ RODA XANTHOMONAS. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
DRUŠTVO ZA ZAŠTITU BILJA SRBIJE., 43.
https://hdl.handle.net/21.15107/rcub_rimsi_1946

Jelušić A, Marković S, Iličić R, Popović Milovanović T. BIOKONTROLNI POTENCIJAL NEKIH VRSTA BACILLUS I PSEUDOMONAS PREMA  PATOGENIMA IZ RODA XANTHOMONAS. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova. 2022;:43.
https://hdl.handle.net/21.15107/rcub_rimsi_1946 .

Jelušić, Aleksandra, Marković, Sanja, Iličić, Renata, Popović Milovanović, Tatjana, "BIOKONTROLNI POTENCIJAL NEKIH VRSTA BACILLUS I PSEUDOMONAS PREMA  PATOGENIMA IZ RODA XANTHOMONAS" in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova (2022):43,
https://hdl.handle.net/21.15107/rcub_rimsi_1946 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - CONF
AU  - Iličić, Renata
AU  - Barać, Goran
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1949
AB  - BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
T1  - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE
SP  - 916
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1949
ER  - 

@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated 
symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were 
frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was 
initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality.
OBJECTIVES
This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) 
in locality Maglić (Bačka) in 2021. 
METHODS 
Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves 
and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium 
bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal 
phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited 
strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to 
reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database.
RESULTS 
PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three 
(two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% 
identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited 
strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE",
pages = "916",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1949"
}

Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949

Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:916.
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA   SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology (2022):916,
https://hdl.handle.net/21.15107/rcub_rimsi_1949 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1951
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)
SP  - 915
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1951
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)",
pages = "915",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1951"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović, T.. (2022). PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović T. PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović, Tatjana, "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):915,
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1914
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.
PB  - Springer
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres
EP  - 18
SP  - 1
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was 
used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected feld 
(58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation 
sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from 
six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present 
in all samples, with Proteobacteria being the most prevalent, especially in tubers 
without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. 
aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were 
more represented in tubers without symptoms. However, the genus Dickeya was at 
the limit of detection or not detected at all. Acinetobacter was the most dominant 
in tubers with soft rot, while Pseudomonas and Enterobacter were the most abun dant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with 
starch-degradable features were almost exclusively present in soft rotted tubers and 
their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, 
Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confrmed biocontrol potential. This study indicates that the soft rot maceration process 
arises due to complex interactions between plant pathogens and other endophytic 
bacteria.",
publisher = "Springer",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres",
pages = "18-1",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research
Springer., 1-18.
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres. in Potato Research. 2022;:1-18.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial  Diversity in Soft Rotting Potato Tubers and Corresponding  Geocaulospheres" in Potato Research (2022):1-18,
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Aćimović, Radivoje
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1958
AB  - The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1958
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1958"
}

Popović, T., Jelušić, A., Aćimović, R., Marković, S.,& Iličić, R.. (2022). Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958

Popović T, Jelušić A, Aćimović R, Marković S, Iličić R. Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .

Popović, Tatjana, Jelušić, Aleksandra, Aćimović, Radivoje, Marković, Sanja, Iličić, Renata, "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):124,
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1959
AB  - In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1959
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2022",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959"
}

Marković, S., Popović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2022). First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959

Marković S, Popović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

Marković, Sanja, Popović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):123,
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

TY  - CONF
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1947
AB  - Bakterija Ralstonia solanacearum, prouzrokovač bakteriozne uvelosti i mrke truleži 
krtola krompira, obzirom na veliki ekonomski značaj i štete koje izaziva ima karantinski 
status u Evropi (EU_2019_2072_AnnexII). U Srbiji je prvi put detektovana 2010. godine, nakon 
čega se i dalje javlja u pojedinim usevima krompira i pored sprovođenja mera eradikacije. 
Prema literaturnim podacima, populacija R. solanacearum identifikovana u Srbiji genetički je 
homogena i identična sa sojevima prisutnim na teritoriji Evrope. Cilj ovog rada je bio da se 
tokom 2021. godine izvrši monitoring krtola krompira na prisustvo mrke truleži i da se izvrši 
identifikacija prouzrokovača bolesti na području Bačke (Vojvodina), gde su uglavnom i 
potvrđivani pozitivni nalazi bakterije R. solanacearum u poslednjoj deceniji. 
Simptomi mrke truleži krtola su utvrđeni kod sorti krompira Pirol i VR808 gajenih na 
lokalitetima Gradina i Lugovo, redom. Izolacija je vršena na SMSA podlogu na kojoj su nakon 5 
dana inkubacije dominirale fluidne kolonije nepravilnog oblika, veličine 2-3 mm, sa crveno 
obojenim centrom i providno-beličastim oreolom oko centra. Jedanaest odabranih izolata je 
preliminarno identifikovano kao R. solanacearum primenom metode lančane reakcije 
polimeraze (PCR) nakon amplifikacije regiona veličine 553 bp korišćenjem para prajmera PS 1/PS-2 i 288 bp sa parom prajmera OLI-1/Y-2 specifičnih za R. solanacearum. Primenom 
specifičnog para prajmera Rs-F/R utvrđeno je da svi izolati pripadaju biovaru 2 R. 
solanacearum. Kao kontrola korišćen je referentni soj R. solanacearum PD2762. Identifikacija 
izolata je dalje potvrđena imunofluorescentnim testom (IF), kojim je dokazano prisustvo 
fluorecscentnih bakterijskih ćelija. Patogenost je potvrđena infiltracijom bakterijske 
suspenzije (107-8 ćel mL-1) u stablo mladih biljaka paradajza (Moneymaker) i krompira (Pirol i 
VR808). Simptomi na paradajzu su se javili u vidu blage uvelosti sa lezijama na mestu 
inokulacije posle 4 dana, a potpuno sušenje je nastupilo 7 dana posle inokulacije. Na 
inokulisanim biljkama krompira prvi simptomi u formi blagog venjenja sa lezijama na mestu 
uboda su se pojavili nakon 6-7 dana. Potpuno propadanje biljaka krompira beleženo je 15 
dana nakon inokulacije. 
Najnovija istraživanja prikazana u ovom radu ukazuju da je bakterija R. solanacearum i 
dalje prisutan patogen krompira u Vojvodini. Detaljna epiodemiološka studija je neophodna 
kako bi se utvrdio potencijalni izvor inokuluma ove bakterije.
PB  - DRUŠTVO ZA ZAŠTITU BILJA SRBIJE
C3  - XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
T1  - RALSTONIA SOLANACEARUM I DALJE PRETNJA PROIZVODNJI KROMPIRA U SRBIJI
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1947
ER  - 

@conference{
author = "Marković, Sanja and Jelušić, Aleksandra and Iličić, Renata and Popović Milovanović, Tatjana",
year = "2022",
abstract = "Bakterija Ralstonia solanacearum, prouzrokovač bakteriozne uvelosti i mrke truleži 
krtola krompira, obzirom na veliki ekonomski značaj i štete koje izaziva ima karantinski 
status u Evropi (EU_2019_2072_AnnexII). U Srbiji je prvi put detektovana 2010. godine, nakon 
čega se i dalje javlja u pojedinim usevima krompira i pored sprovođenja mera eradikacije. 
Prema literaturnim podacima, populacija R. solanacearum identifikovana u Srbiji genetički je 
homogena i identična sa sojevima prisutnim na teritoriji Evrope. Cilj ovog rada je bio da se 
tokom 2021. godine izvrši monitoring krtola krompira na prisustvo mrke truleži i da se izvrši 
identifikacija prouzrokovača bolesti na području Bačke (Vojvodina), gde su uglavnom i 
potvrđivani pozitivni nalazi bakterije R. solanacearum u poslednjoj deceniji. 
Simptomi mrke truleži krtola su utvrđeni kod sorti krompira Pirol i VR808 gajenih na 
lokalitetima Gradina i Lugovo, redom. Izolacija je vršena na SMSA podlogu na kojoj su nakon 5 
dana inkubacije dominirale fluidne kolonije nepravilnog oblika, veličine 2-3 mm, sa crveno 
obojenim centrom i providno-beličastim oreolom oko centra. Jedanaest odabranih izolata je 
preliminarno identifikovano kao R. solanacearum primenom metode lančane reakcije 
polimeraze (PCR) nakon amplifikacije regiona veličine 553 bp korišćenjem para prajmera PS 1/PS-2 i 288 bp sa parom prajmera OLI-1/Y-2 specifičnih za R. solanacearum. Primenom 
specifičnog para prajmera Rs-F/R utvrđeno je da svi izolati pripadaju biovaru 2 R. 
solanacearum. Kao kontrola korišćen je referentni soj R. solanacearum PD2762. Identifikacija 
izolata je dalje potvrđena imunofluorescentnim testom (IF), kojim je dokazano prisustvo 
fluorecscentnih bakterijskih ćelija. Patogenost je potvrđena infiltracijom bakterijske 
suspenzije (107-8 ćel mL-1) u stablo mladih biljaka paradajza (Moneymaker) i krompira (Pirol i 
VR808). Simptomi na paradajzu su se javili u vidu blage uvelosti sa lezijama na mestu 
inokulacije posle 4 dana, a potpuno sušenje je nastupilo 7 dana posle inokulacije. Na 
inokulisanim biljkama krompira prvi simptomi u formi blagog venjenja sa lezijama na mestu 
uboda su se pojavili nakon 6-7 dana. Potpuno propadanje biljaka krompira beleženo je 15 
dana nakon inokulacije. 
Najnovija istraživanja prikazana u ovom radu ukazuju da je bakterija R. solanacearum i 
dalje prisutan patogen krompira u Vojvodini. Detaljna epiodemiološka studija je neophodna 
kako bi se utvrdio potencijalni izvor inokuluma ove bakterije.",
publisher = "DRUŠTVO ZA ZAŠTITU BILJA SRBIJE",
journal = "XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova",
title = "RALSTONIA SOLANACEARUM I DALJE PRETNJA PROIZVODNJI KROMPIRA U SRBIJI",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1947"
}

Marković, S., Jelušić, A., Iličić, R.,& Popović Milovanović, T.. (2022). RALSTONIA SOLANACEARUM I DALJE PRETNJA PROIZVODNJI KROMPIRA U SRBIJI. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
DRUŠTVO ZA ZAŠTITU BILJA SRBIJE., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1947

Marković S, Jelušić A, Iličić R, Popović Milovanović T. RALSTONIA SOLANACEARUM I DALJE PRETNJA PROIZVODNJI KROMPIRA U SRBIJI. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova. 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1947 .

Marković, Sanja, Jelušić, Aleksandra, Iličić, Renata, Popović Milovanović, Tatjana, "RALSTONIA SOLANACEARUM I DALJE PRETNJA PROIZVODNJI KROMPIRA U SRBIJI" in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1947 .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
AU  - Zečević, Katarina
AU  - Trkulja, Nenad
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1948
AB  - Fitopatogena bakterija Acidovorax citrulli, prouzrokovač bakteriozne mrljavosti 
plodova lubenice prvi put je detektovana u Srbiji 2014. godine u Bačkoj i Sremu. Zbog 
ekonomskog značaja u regionu Evropske unije je svrstana na A1 listu karantinskih 
organizama (Evropska i mediteranska organizacija za zaštitu bilja), a u Srbiji na Listu IA deo I 
(Službeni glasnik 57/15). Distribucija zaraženog semena predstavlja najvažniji izvor 
inokuluma A. citrulli, pa se kontrola patogena prvenstveno bazira na testiranje zdravstvenog 
stanja semena. Osim ove mere preporučuje se i uklanjanje potencijalnih domaćina ove 
bakterije u polju (samoniklih i korovskih vrsta Cucurbitacae), uvođenje plodoreda, kao i 
hemijska zaštita fungicidima na bazi bakra. Međutim, iako se u Srbiji redovno sprovode mere 
eradikacije na lokalitetima gde je utvrđeno prisustvo A. citrulli, kao i redovna kontrola semena 
iz uvoza i program mera za zaštitu zdravlja bilja, simptomi bolesti se u pojedinim lokalitetima 
i dalje javljaju. Stoga, cilj ovog rada je praćenje, izolacija i identifikacija A. citrulli na plodovima 
lubenice sa simptomima bakteriozne mrljavosti u dvogodišnjem periodu (2021-2022. 
godina). 
Tokom 2021. na lokalitetu Ašanja i 2022. godine na lokalitetu Hrtkovci beleženi su 
simptomi bakteriozne mrljavosti lubenice. Izolacija prouzrokovača je vršena na hranljivu 
podlogu obogaćenu 5% saharozom. Brojne bakterijske kolonije, među kojima su dominirale 
sitne, beličaste, okrugle, sjajne i ispupčene kolonije formirale su se nakon tri dana inkubacije. 
Za rad je odabrano 10 izolata po godini/lokalitetu, a kao uporedni sojevi poslužili su A. citrulli 
dobijeni 2014. (lokalitet Čelarevo) i 2018. (lokalitet Šabac). Patogenost je dokazana na 
plodovima lubenice, pojavom tamno-zelenih, vlažnih pega 3-5 dana nakon injektiranja 
bakterijske suspenzije u mezokarp. Metodom lančane reakcije polimeraze (PCR) korišćenjem 
A. citrulli specifičnog para prajmera BX-L1/BX-S-R2 amplifikovani su fragmenti veličine 279 
bp kod svih ispitivanih i uporenih izolata. Na osnovu profila generisanih umnožavanjem 
ponavljajućih sekvenci DNK (rep-PCR) prajmerom za (GTG)5-PCR i nasumičnom 
amplifikacijom polimorfne DNK (RAPD-PCR) prajmerom za M13-PCR, dokazana je genetička 
heterogenost, odnosno izdvajanje izolata dobijenih takom 2021. (I grupa) i 2022. (II grupa) 
godine u dve grupe, dok su uporedni izolati iz 2014. i 2018. god. svrstani u grupu III. Nasuprot 
tome, BOX-PCR je ukazao na homogenost izolata bez obzira na godinu izolacije. Dalja 
ispitivanja će biti usmerena na genotipizaciju sekvenci više genskih lokusa, kako bi se utvrdio 
potencijalni diverzitet novih izolata A. citrulli, i eventualno putevi introdukcije i širenja ovog 
patogena u Srbiji.
PB  - DRUŠTVO ZA ZAŠTITU BILJA SRBIJE
C3  - XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
T1  - ACIDOVORAX CITRULLI – RIZIK OD DALJEG ŠIRENJA U SRBIJI
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1948
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Iličić, Renata and Zečević, Katarina and Trkulja, Nenad and Marković, Sanja and Jelušić, Aleksandra and Milovanović, Predrag",
year = "2022",
abstract = "Fitopatogena bakterija Acidovorax citrulli, prouzrokovač bakteriozne mrljavosti 
plodova lubenice prvi put je detektovana u Srbiji 2014. godine u Bačkoj i Sremu. Zbog 
ekonomskog značaja u regionu Evropske unije je svrstana na A1 listu karantinskih 
organizama (Evropska i mediteranska organizacija za zaštitu bilja), a u Srbiji na Listu IA deo I 
(Službeni glasnik 57/15). Distribucija zaraženog semena predstavlja najvažniji izvor 
inokuluma A. citrulli, pa se kontrola patogena prvenstveno bazira na testiranje zdravstvenog 
stanja semena. Osim ove mere preporučuje se i uklanjanje potencijalnih domaćina ove 
bakterije u polju (samoniklih i korovskih vrsta Cucurbitacae), uvođenje plodoreda, kao i 
hemijska zaštita fungicidima na bazi bakra. Međutim, iako se u Srbiji redovno sprovode mere 
eradikacije na lokalitetima gde je utvrđeno prisustvo A. citrulli, kao i redovna kontrola semena 
iz uvoza i program mera za zaštitu zdravlja bilja, simptomi bolesti se u pojedinim lokalitetima 
i dalje javljaju. Stoga, cilj ovog rada je praćenje, izolacija i identifikacija A. citrulli na plodovima 
lubenice sa simptomima bakteriozne mrljavosti u dvogodišnjem periodu (2021-2022. 
godina). 
Tokom 2021. na lokalitetu Ašanja i 2022. godine na lokalitetu Hrtkovci beleženi su 
simptomi bakteriozne mrljavosti lubenice. Izolacija prouzrokovača je vršena na hranljivu 
podlogu obogaćenu 5% saharozom. Brojne bakterijske kolonije, među kojima su dominirale 
sitne, beličaste, okrugle, sjajne i ispupčene kolonije formirale su se nakon tri dana inkubacije. 
Za rad je odabrano 10 izolata po godini/lokalitetu, a kao uporedni sojevi poslužili su A. citrulli 
dobijeni 2014. (lokalitet Čelarevo) i 2018. (lokalitet Šabac). Patogenost je dokazana na 
plodovima lubenice, pojavom tamno-zelenih, vlažnih pega 3-5 dana nakon injektiranja 
bakterijske suspenzije u mezokarp. Metodom lančane reakcije polimeraze (PCR) korišćenjem 
A. citrulli specifičnog para prajmera BX-L1/BX-S-R2 amplifikovani su fragmenti veličine 279 
bp kod svih ispitivanih i uporenih izolata. Na osnovu profila generisanih umnožavanjem 
ponavljajućih sekvenci DNK (rep-PCR) prajmerom za (GTG)5-PCR i nasumičnom 
amplifikacijom polimorfne DNK (RAPD-PCR) prajmerom za M13-PCR, dokazana je genetička 
heterogenost, odnosno izdvajanje izolata dobijenih takom 2021. (I grupa) i 2022. (II grupa) 
godine u dve grupe, dok su uporedni izolati iz 2014. i 2018. god. svrstani u grupu III. Nasuprot 
tome, BOX-PCR je ukazao na homogenost izolata bez obzira na godinu izolacije. Dalja 
ispitivanja će biti usmerena na genotipizaciju sekvenci više genskih lokusa, kako bi se utvrdio 
potencijalni diverzitet novih izolata A. citrulli, i eventualno putevi introdukcije i širenja ovog 
patogena u Srbiji.",
publisher = "DRUŠTVO ZA ZAŠTITU BILJA SRBIJE",
journal = "XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova",
title = "ACIDOVORAX CITRULLI – RIZIK OD DALJEG ŠIRENJA U SRBIJI",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1948"
}

Popović Milovanović, T., Iličić, R., Zečević, K., Trkulja, N., Marković, S., Jelušić, A.,& Milovanović, P.. (2022). ACIDOVORAX CITRULLI – RIZIK OD DALJEG ŠIRENJA U SRBIJI. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova
DRUŠTVO ZA ZAŠTITU BILJA SRBIJE., 41.
https://hdl.handle.net/21.15107/rcub_rimsi_1948

Popović Milovanović T, Iličić R, Zečević K, Trkulja N, Marković S, Jelušić A, Milovanović P. ACIDOVORAX CITRULLI – RIZIK OD DALJEG ŠIRENJA U SRBIJI. in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova. 2022;:41.
https://hdl.handle.net/21.15107/rcub_rimsi_1948 .

Popović Milovanović, Tatjana, Iličić, Renata, Zečević, Katarina, Trkulja, Nenad, Marković, Sanja, Jelušić, Aleksandra, Milovanović, Predrag, "ACIDOVORAX CITRULLI – RIZIK OD DALJEG ŠIRENJA U SRBIJI" in XVII SAVETOVANJE O ZAŠTITI BILJA  Zbornik rezimea radova (2022):41,
https://hdl.handle.net/21.15107/rcub_rimsi_1948 .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Barac, Goran
AU  - Bagi, Ferenc
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1531
AB  - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
EP  - 150
IS  - 1
SP  - 140
VL  - 180
DO  - 10.1111/aab.12717
ER  - 

@article{
author = "Ilicic, Renata and Jelušić, Aleksandra and Marković, Sanja and Barac, Goran and Bagi, Ferenc and Popović, Tatjana",
year = "2022",
abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification",
pages = "150-140",
number = "1",
volume = "180",
doi = "10.1111/aab.12717"
}

Ilicic, R., Jelušić, A., Marković, S., Barac, G., Bagi, F.,& Popović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology
Wiley, Hoboken., 180(1), 140-150.
https://doi.org/10.1111/aab.12717

Ilicic R, Jelušić A, Marković S, Barac G, Bagi F, Popović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150.
doi:10.1111/aab.12717 .

Ilicic, Renata, Jelušić, Aleksandra, Marković, Sanja, Barac, Goran, Bagi, Ferenc, Popović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150,
https://doi.org/10.1111/aab.12717 . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Iličić, Renata
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1957
AB  - Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i 
plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena 
fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske 
organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I 
(Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog 
značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. 
armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na 
području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. 
na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u 
gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički 
haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa 
različitih kontinenata i biljnih domaćina. 
U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 
10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze
(PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. 
Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna 
sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za 
pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) 
zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, 
Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i 
Španije). 
Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su 
homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz 
Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, 
dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu 
II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. 
Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, 
obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni 
genotip bakterije.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1957
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Milovanović, Predrag and Marković, Sanja and Blagojević, Milan and Iličić, Renata",
year = "2021",
abstract = "Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i 
plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena 
fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske 
organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I 
(Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog 
značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. 
armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na 
području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. 
na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u 
gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički 
haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa 
različitih kontinenata i biljnih domaćina. 
U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 
10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze
(PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. 
Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna 
sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za 
pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) 
zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, 
Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i 
Španije). 
Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su 
homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz 
Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, 
dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu 
II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. 
Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, 
obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni 
genotip bakterije.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1957"
}

Popović, T., Jelušić, A., Milovanović, P., Marković, S., Blagojević, M.,& Iličić, R.. (2021). DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 44.
https://hdl.handle.net/21.15107/rcub_rimsi_1957

Popović T, Jelušić A, Milovanović P, Marković S, Blagojević M, Iličić R. DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:44.
https://hdl.handle.net/21.15107/rcub_rimsi_1957 .

Popović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Marković, Sanja, Blagojević, Milan, Iličić, Renata, "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA  BRESKVE I KAJSIJE U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):44,
https://hdl.handle.net/21.15107/rcub_rimsi_1957 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Ilicic, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1438
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Ilicic, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421"
}

Marković, S., Stanković, S., Ilicic, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
Wiley, Hoboken., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Ilicic R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Ilicic, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Iličić, Renata
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1980
AB  - Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne 
pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri 
povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi 
zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum
prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj 
ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa 
obolelih listova trešnje i šljive.
U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive 
(lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize 
multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i 
rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane 
reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u 
trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C 
(gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i 
finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene 
sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu 
pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa 
trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne 
pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. 
syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi).
PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 
bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na 
veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih 
gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što 
ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu 
konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno 
sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. 
morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. 
Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su 
odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s.
pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, 
domaćina ili lokalitet sa kog su izolovani.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1980
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Marković, Sanja and Blagojević, Milan and Bagi, Ferenc and Iličić, Renata",
year = "2021",
abstract = "Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne 
pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri 
povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi 
zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum
prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj 
ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa 
obolelih listova trešnje i šljive.
U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive 
(lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize 
multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i 
rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane 
reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u 
trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C 
(gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i 
finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene 
sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu 
pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa 
trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne 
pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. 
syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi).
PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 
bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na 
veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih 
gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što 
ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu 
konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno 
sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. 
morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. 
Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su 
odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s.
pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, 
domaćina ili lokalitet sa kog su izolovani.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1980"
}

Jelušić, A., Popović, T., Marković, S., Blagojević, M., Bagi, F.,& Iličić, R.. (2021). MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 45.
https://hdl.handle.net/21.15107/rcub_rimsi_1980

Jelušić A, Popović T, Marković S, Blagojević M, Bagi F, Iličić R. MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_1980 .

Jelušić, Aleksandra, Popović, Tatjana, Marković, Sanja, Blagojević, Milan, Bagi, Ferenc, Iličić, Renata, "MOLEKULARNA KARAKTERIZACIJA Pseudomonas syringae pv. morsprunorum POREKLOM SA TREŠNJE I ŠLJIVE U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):45,
https://hdl.handle.net/21.15107/rcub_rimsi_1980 .

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1961
AB  - Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
T1  - NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1961
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2021",
abstract = "Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, 
jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 
1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium 
atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na 
sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima 
i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši 
identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi 
distribucija populacija u 2020. i 2021. godini.
Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 
uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa 
sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 
ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti 
VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste 
kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira 
i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze 
(PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti 
su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa 
krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano 
(MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. 
Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su 
formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata 
izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama 
krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na 
mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od 
inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, 
izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. 
brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati 
prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata 
sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja 
crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je 
trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova",
title = "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1961"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2021). NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova
Društvo za zaštitu bilja Srbije., 17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961

Marković S, Stanković S, Jelušić A, Iličić R, Popović T. NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova. 2021;:17.
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova (2021):17,
https://hdl.handle.net/21.15107/rcub_rimsi_1961 .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Kosovac, Andrea
AU  - Postic, Dobrivoj
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1494
AB  - Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia
EP  - 1090
IS  - 4
SP  - 1080
VL  - 105
DO  - 10.1094/PDIS-05-20-1076-RE
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Ilicic, Renata and Kosovac, Andrea and Postic, Dobrivoj and Popović, Tatjana",
year = "2021",
abstract = "Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia",
pages = "1090-1080",
number = "4",
volume = "105",
doi = "10.1094/PDIS-05-20-1076-RE"
}

Marković, S., Stanković, S., Jelušić, A., Ilicic, R., Kosovac, A., Postic, D.,& Popović, T.. (2021). Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 105(4), 1080-1090.
https://doi.org/10.1094/PDIS-05-20-1076-RE

Marković S, Stanković S, Jelušić A, Ilicic R, Kosovac A, Postic D, Popović T. Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease. 2021;105(4):1080-1090.
doi:10.1094/PDIS-05-20-1076-RE .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Ilicic, Renata, Kosovac, Andrea, Postic, Dobrivoj, Popović, Tatjana, "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia" in Plant Disease, 105, no. 4 (2021):1080-1090,
https://doi.org/10.1094/PDIS-05-20-1076-RE . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajic, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1419
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Ilicic, Renata and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajic, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Ilicic, R., Popović, T., Marković, S., Jelušić, A., Bagi, F., Vlajic, S.,& Stanković, S.. (2021). Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Ilicic R, Popović T, Marković S, Jelušić A, Bagi F, Vlajic S, Stanković S. Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Ilicic, Renata, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajic, Slobodan, Stanković, Slaviša, "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika-Belgrade, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Broćić, Zoran
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanisavljević, Rade
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1917
AB  - The aim of this study was to determine the influence of the origin and size of planting material on the morphological 
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and 
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected 
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40 
and 60 g, compared to the larger fraction of 80 g.
PB  - National Society of Processing and Energy in Agriculture, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS
EP  - 23
IS  - 1
SP  - 20
VL  - 25
DO  - 10.5937/jpea25-30772
ER  - 

@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Broćić, Zoran and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Stanisavljević, Rade",
year = "2021",
abstract = "The aim of this study was to determine the influence of the origin and size of planting material on the morphological 
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and 
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected 
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40 
and 60 g, compared to the larger fraction of 80 g.",
publisher = "National Society of Processing and Energy in Agriculture, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS",
pages = "23-20",
number = "1",
volume = "25",
doi = "10.5937/jpea25-30772"
}

Poštić, D., Štrbanović, R., Broćić, Z., Popović, T., Marković, S., Jelušić, A.,& Stanisavljević, R.. (2021). INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS. in Journal on Processing and Energy in Agriculture
National Society of Processing and Energy in Agriculture, Novi Sad., 25(1), 20-23.
https://doi.org/10.5937/jpea25-30772

Poštić D, Štrbanović R, Broćić Z, Popović T, Marković S, Jelušić A, Stanisavljević R. INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS. in Journal on Processing and Energy in Agriculture. 2021;25(1):20-23.
doi:10.5937/jpea25-30772 .

Poštić, Dobrivoj, Štrbanović, Ratibor, Broćić, Zoran, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Stanisavljević, Rade, "INFLUENCE OF ORIGIN AND SIZE OF POTATO PLANTING MATERIAL  ON MORPHOLOGICAL CHARACTERISTICS OF SEED TUBERS" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):20-23,
https://doi.org/10.5937/jpea25-30772 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović, Tatjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950
AB  - Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1950
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana",
year = "2020",
abstract = "Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950"
}

Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950

Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133,
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1954
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1954
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and 
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families 
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins. 
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential 
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes. 
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced. 
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R, 
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases 
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chro matography-mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus 
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and 
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain 
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp. 
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1954"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954

Jelušić A, Popović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology. 2020;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS  VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology (2020):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1954 .