TY  - CONF
AU  - Zmejkoski, Danica Z.
AU  - Spasojević, Dragica
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Orlovska, Irina
AU  - Kozirovska, Natalia
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2913
AB  - Biofilm-forming bacteria are recognized as a major impediment to wound healing. The aim of this study was to improve design of the formulations used for care of biofilm-infected wounds. Since lignins and lignin-derived compounds are known to have antibacterial properties especially efficient against biofilm-forming pathogens, the gel and membrane type of materials of enzymatically synthesized lignin model compound, dehydrogenative polymer of coniferyl alcohol (DHP) with alginate (Alg) and bacterial cellulose (BC) synthesized by culture Komagataeibacter intermedius IMBG180, were composed. The hydrogels were tested on Gram-negative P. aeruginosa (ATCC 27853) and S. typhimurium (ATCC 13311), Gram-positive bacteria L. monocytogenes (NCTC 7973) and S. aureus (ATCC 6538), as well as S. aureus #1*, S. aureus #2*, P. aeruginosa* and Serratia sp.* clinical human isolates obtained from patients’ wounds. DHP-Alg showed activity against all ofthe tested bacteria with minimal inhibitory concentrations (MICs) of 0.002–0.45 mg/mL and minimal bactericidal concentrations (MBCs) of 0.004–0.90 mg/mL. L. monocytogenes was the most susceptible. DHP-BC exhibited antibacterial activity on all tested bacteria, with exception of P. aeruginosa and S. aureus #1*. MICs and MBCs were in range of 0.22-0.42 mg/mL and 0.22-0.88 mg/mL, respectively. DHP-Alg gel and DHP-BC membranes, after initial release in the first hour of 34% and 19.26% of DHP, respectively, allowed the slow and continuous release of DHP in PBS solution. After 24 hours the amount of released antimicrobial substance from DHP-Alg and DHP-BC were 42.13% and 46.2%, respectively. Based on all obtained results, both hydrogels have the potential to be used in medical treatments as antibacterial agents.
PB  - Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје
C3  - 25th Congress of SCTM
T1  - Antimicrobial composite polymers as potential agents in chronic wound healing
SP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2913
ER  - 

@conference{
author = "Zmejkoski, Danica Z. and Spasojević, Dragica and Soković, Marina and Glamočlija, Jasmina and Orlovska, Irina and Kozirovska, Natalia and Radotić, Ksenija",
year = "2018",
abstract = "Biofilm-forming bacteria are recognized as a major impediment to wound healing. The aim of this study was to improve design of the formulations used for care of biofilm-infected wounds. Since lignins and lignin-derived compounds are known to have antibacterial properties especially efficient against biofilm-forming pathogens, the gel and membrane type of materials of enzymatically synthesized lignin model compound, dehydrogenative polymer of coniferyl alcohol (DHP) with alginate (Alg) and bacterial cellulose (BC) synthesized by culture Komagataeibacter intermedius IMBG180, were composed. The hydrogels were tested on Gram-negative P. aeruginosa (ATCC 27853) and S. typhimurium (ATCC 13311), Gram-positive bacteria L. monocytogenes (NCTC 7973) and S. aureus (ATCC 6538), as well as S. aureus #1*, S. aureus #2*, P. aeruginosa* and Serratia sp.* clinical human isolates obtained from patients’ wounds. DHP-Alg showed activity against all ofthe tested bacteria with minimal inhibitory concentrations (MICs) of 0.002–0.45 mg/mL and minimal bactericidal concentrations (MBCs) of 0.004–0.90 mg/mL. L. monocytogenes was the most susceptible. DHP-BC exhibited antibacterial activity on all tested bacteria, with exception of P. aeruginosa and S. aureus #1*. MICs and MBCs were in range of 0.22-0.42 mg/mL and 0.22-0.88 mg/mL, respectively. DHP-Alg gel and DHP-BC membranes, after initial release in the first hour of 34% and 19.26% of DHP, respectively, allowed the slow and continuous release of DHP in PBS solution. After 24 hours the amount of released antimicrobial substance from DHP-Alg and DHP-BC were 42.13% and 46.2%, respectively. Based on all obtained results, both hydrogels have the potential to be used in medical treatments as antibacterial agents.",
publisher = "Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје",
journal = "25th Congress of SCTM",
title = "Antimicrobial composite polymers as potential agents in chronic wound healing",
pages = "223",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2913"
}

Zmejkoski, D. Z., Spasojević, D., Soković, M., Glamočlija, J., Orlovska, I., Kozirovska, N.,& Radotić, K.. (2018). Antimicrobial composite polymers as potential agents in chronic wound healing. in 25th Congress of SCTM
Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје., 223.
https://hdl.handle.net/21.15107/rcub_rimsi_2913

Zmejkoski DZ, Spasojević D, Soković M, Glamočlija J, Orlovska I, Kozirovska N, Radotić K. Antimicrobial composite polymers as potential agents in chronic wound healing. in 25th Congress of SCTM. 2018;:223.
https://hdl.handle.net/21.15107/rcub_rimsi_2913 .

Zmejkoski, Danica Z., Spasojević, Dragica, Soković, Marina, Glamočlija, Jasmina, Orlovska, Irina, Kozirovska, Natalia, Radotić, Ksenija, "Antimicrobial composite polymers as potential agents in chronic wound healing" in 25th Congress of SCTM (2018):223,
https://hdl.handle.net/21.15107/rcub_rimsi_2913 .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina
AU  - Kozyrovska, Natalia
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola
AU  - Maksimović, Vuk
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1157
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
EP  - 503
SP  - 494
VL  - 118
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina and Kozyrovska, Natalia and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola and Maksimović, Vuk and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
pages = "503-494",
volume = "118",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I., Kozyrovska, N., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N., Maksimović, V., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 118, 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska I, Kozyrovska N, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić N, Maksimović V, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;118:494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina, Kozyrovska, Natalia, Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola, Maksimović, Vuk, Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules, 118 (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .