Antimicrobial composite polymers as potential agents in chronic wound healing
Аутори
Zmejkoski, Danica Z.Spasojević, Dragica
Soković, Marina
Glamočlija, Jasmina
Orlovska, Irina
Kozirovska, Natalia
Radotić, Ksenija
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Biofilm-forming bacteria are recognized as a major impediment to wound healing. The aim of this study was to improve design of the formulations used for care of biofilm-infected wounds. Since lignins and lignin-derived compounds are known to have antibacterial properties especially efficient against biofilm-forming pathogens, the gel and membrane type of materials of enzymatically synthesized lignin model compound, dehydrogenative polymer of coniferyl alcohol (DHP) with alginate (Alg) and bacterial cellulose (BC) synthesized by culture Komagataeibacter intermedius IMBG180, were composed. The hydrogels were tested on Gram-negative P. aeruginosa (ATCC 27853) and S. typhimurium (ATCC 13311), Gram-positive bacteria L. monocytogenes (NCTC 7973) and S. aureus (ATCC 6538), as well as S. aureus #1*, S. aureus #2*, P. aeruginosa* and Serratia sp.* clinical human isolates obtained from patients’ wounds. DHP-Alg showed activity against all ofthe tested bacteria with minimal inhibitory concentrati...ons (MICs) of 0.002–0.45 mg/mL and minimal bactericidal concentrations (MBCs) of 0.004–0.90 mg/mL. L. monocytogenes was the most susceptible. DHP-BC exhibited antibacterial activity on all tested bacteria, with exception of P. aeruginosa and S. aureus #1*. MICs and MBCs were in range of 0.22-0.42 mg/mL and 0.22-0.88 mg/mL, respectively. DHP-Alg gel and DHP-BC membranes, after initial release in the first hour of 34% and 19.26% of DHP, respectively, allowed the slow and continuous release of DHP in PBS solution. After 24 hours the amount of released antimicrobial substance from DHP-Alg and DHP-BC were 42.13% and 46.2%, respectively. Based on all obtained results, both hydrogels have the potential to be used in medical treatments as antibacterial agents.
Кључне речи:
lignin model polymer / bacterial cellulose / antimicrobial activity / wound healingИзвор:
25th Congress of SCTM, 2018, 223-Издавач:
- Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Синтеза, процесирање и карактеризација наноструктурних материјала за примену у области енергије, механичког инжењерства, заштите животне стредине и биомедицине (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Zmejkoski, Danica Z. AU - Spasojević, Dragica AU - Soković, Marina AU - Glamočlija, Jasmina AU - Orlovska, Irina AU - Kozirovska, Natalia AU - Radotić, Ksenija PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2913 AB - Biofilm-forming bacteria are recognized as a major impediment to wound healing. The aim of this study was to improve design of the formulations used for care of biofilm-infected wounds. Since lignins and lignin-derived compounds are known to have antibacterial properties especially efficient against biofilm-forming pathogens, the gel and membrane type of materials of enzymatically synthesized lignin model compound, dehydrogenative polymer of coniferyl alcohol (DHP) with alginate (Alg) and bacterial cellulose (BC) synthesized by culture Komagataeibacter intermedius IMBG180, were composed. The hydrogels were tested on Gram-negative P. aeruginosa (ATCC 27853) and S. typhimurium (ATCC 13311), Gram-positive bacteria L. monocytogenes (NCTC 7973) and S. aureus (ATCC 6538), as well as S. aureus #1*, S. aureus #2*, P. aeruginosa* and Serratia sp.* clinical human isolates obtained from patients’ wounds. DHP-Alg showed activity against all ofthe tested bacteria with minimal inhibitory concentrations (MICs) of 0.002–0.45 mg/mL and minimal bactericidal concentrations (MBCs) of 0.004–0.90 mg/mL. L. monocytogenes was the most susceptible. DHP-BC exhibited antibacterial activity on all tested bacteria, with exception of P. aeruginosa and S. aureus #1*. MICs and MBCs were in range of 0.22-0.42 mg/mL and 0.22-0.88 mg/mL, respectively. DHP-Alg gel and DHP-BC membranes, after initial release in the first hour of 34% and 19.26% of DHP, respectively, allowed the slow and continuous release of DHP in PBS solution. After 24 hours the amount of released antimicrobial substance from DHP-Alg and DHP-BC were 42.13% and 46.2%, respectively. Based on all obtained results, both hydrogels have the potential to be used in medical treatments as antibacterial agents. PB - Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје C3 - 25th Congress of SCTM T1 - Antimicrobial composite polymers as potential agents in chronic wound healing SP - 223 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2913 ER -
@conference{ author = "Zmejkoski, Danica Z. and Spasojević, Dragica and Soković, Marina and Glamočlija, Jasmina and Orlovska, Irina and Kozirovska, Natalia and Radotić, Ksenija", year = "2018", abstract = "Biofilm-forming bacteria are recognized as a major impediment to wound healing. The aim of this study was to improve design of the formulations used for care of biofilm-infected wounds. Since lignins and lignin-derived compounds are known to have antibacterial properties especially efficient against biofilm-forming pathogens, the gel and membrane type of materials of enzymatically synthesized lignin model compound, dehydrogenative polymer of coniferyl alcohol (DHP) with alginate (Alg) and bacterial cellulose (BC) synthesized by culture Komagataeibacter intermedius IMBG180, were composed. The hydrogels were tested on Gram-negative P. aeruginosa (ATCC 27853) and S. typhimurium (ATCC 13311), Gram-positive bacteria L. monocytogenes (NCTC 7973) and S. aureus (ATCC 6538), as well as S. aureus #1*, S. aureus #2*, P. aeruginosa* and Serratia sp.* clinical human isolates obtained from patients’ wounds. DHP-Alg showed activity against all ofthe tested bacteria with minimal inhibitory concentrations (MICs) of 0.002–0.45 mg/mL and minimal bactericidal concentrations (MBCs) of 0.004–0.90 mg/mL. L. monocytogenes was the most susceptible. DHP-BC exhibited antibacterial activity on all tested bacteria, with exception of P. aeruginosa and S. aureus #1*. MICs and MBCs were in range of 0.22-0.42 mg/mL and 0.22-0.88 mg/mL, respectively. DHP-Alg gel and DHP-BC membranes, after initial release in the first hour of 34% and 19.26% of DHP, respectively, allowed the slow and continuous release of DHP in PBS solution. After 24 hours the amount of released antimicrobial substance from DHP-Alg and DHP-BC were 42.13% and 46.2%, respectively. Based on all obtained results, both hydrogels have the potential to be used in medical treatments as antibacterial agents.", publisher = "Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје", journal = "25th Congress of SCTM", title = "Antimicrobial composite polymers as potential agents in chronic wound healing", pages = "223", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2913" }
Zmejkoski, D. Z., Spasojević, D., Soković, M., Glamočlija, J., Orlovska, I., Kozirovska, N.,& Radotić, K.. (2018). Antimicrobial composite polymers as potential agents in chronic wound healing. in 25th Congress of SCTM Национална и универзитетска библиотека „Св. Климент Охридски“, Скопје., 223. https://hdl.handle.net/21.15107/rcub_rimsi_2913
Zmejkoski DZ, Spasojević D, Soković M, Glamočlija J, Orlovska I, Kozirovska N, Radotić K. Antimicrobial composite polymers as potential agents in chronic wound healing. in 25th Congress of SCTM. 2018;:223. https://hdl.handle.net/21.15107/rcub_rimsi_2913 .
Zmejkoski, Danica Z., Spasojević, Dragica, Soković, Marina, Glamočlija, Jasmina, Orlovska, Irina, Kozirovska, Natalia, Radotić, Ksenija, "Antimicrobial composite polymers as potential agents in chronic wound healing" in 25th Congress of SCTM (2018):223, https://hdl.handle.net/21.15107/rcub_rimsi_2913 .