TY  - JOUR
AU  - Radotić, Ksenija
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Tanasijević, Gordana
AU  - Maksimović, Vuk
AU  - Dragišić Maksimović, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1524
AB  - Silicon (Si) is considered a beneficial element for plants, mostly accumulating in cell walls, where its location and content are primed by the chemistry and structure of lignin. It is unrevealed how Si interacts with the process of lignin formation in the CWs. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase-catalyzed polymerization of a lignin monomer into the lignin model compound, imitating conditions of the last step of lignin formation. FTIR and fluorescence spectroscopy and microscopy showed that Si is bound to the final polymer, and the structure of the Si-DHP differs from pure DHP. Fluorescence spectroscopy showed that Si does not bind to the monomers, so Si probably inhibits the formation of the larger lignin fragments, as evidenced by HPLC-DAD, by binding to dimmers formed during DHP synthesis. The structural changes of the polymer are related to the changed proportion of the fractions of various MW. The enzyme catalyzing DHP synthesis was not inhibited by Si. HRP activity was increased in presence of Si except for 6 mM Si. This may indicate that the complex formed with Si and short oligomers activates the enzyme, and prevents the formation of the large fragments.
PB  - Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Influence of silicon on polymerization process during lignin synthesis. Implications for cell wall properties
EP  - 174
SP  - 168
VL  - 198
DO  - 10.1016/j.ijbiomac.2021.12.143
ER  - 

@article{
author = "Radotić, Ksenija and Đikanović, Daniela and Kalauzi, Aleksandar and Tanasijević, Gordana and Maksimović, Vuk and Dragišić Maksimović, Jelena",
year = "2022",
abstract = "Silicon (Si) is considered a beneficial element for plants, mostly accumulating in cell walls, where its location and content are primed by the chemistry and structure of lignin. It is unrevealed how Si interacts with the process of lignin formation in the CWs. We studied, in an in vitro system, the interaction of SiO2 with the peroxidase-catalyzed polymerization of a lignin monomer into the lignin model compound, imitating conditions of the last step of lignin formation. FTIR and fluorescence spectroscopy and microscopy showed that Si is bound to the final polymer, and the structure of the Si-DHP differs from pure DHP. Fluorescence spectroscopy showed that Si does not bind to the monomers, so Si probably inhibits the formation of the larger lignin fragments, as evidenced by HPLC-DAD, by binding to dimmers formed during DHP synthesis. The structural changes of the polymer are related to the changed proportion of the fractions of various MW. The enzyme catalyzing DHP synthesis was not inhibited by Si. HRP activity was increased in presence of Si except for 6 mM Si. This may indicate that the complex formed with Si and short oligomers activates the enzyme, and prevents the formation of the large fragments.",
publisher = "Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Influence of silicon on polymerization process during lignin synthesis. Implications for cell wall properties",
pages = "174-168",
volume = "198",
doi = "10.1016/j.ijbiomac.2021.12.143"
}

Radotić, K., Đikanović, D., Kalauzi, A., Tanasijević, G., Maksimović, V.,& Dragišić Maksimović, J.. (2022). Influence of silicon on polymerization process during lignin synthesis. Implications for cell wall properties. in International Journal of Biological Macromolecules
Elsevier B.V.., 198, 168-174.
https://doi.org/10.1016/j.ijbiomac.2021.12.143

Radotić K, Đikanović D, Kalauzi A, Tanasijević G, Maksimović V, Dragišić Maksimović J. Influence of silicon on polymerization process during lignin synthesis. Implications for cell wall properties. in International Journal of Biological Macromolecules. 2022;198:168-174.
doi:10.1016/j.ijbiomac.2021.12.143 .

Radotić, Ksenija, Đikanović, Daniela, Kalauzi, Aleksandar, Tanasijević, Gordana, Maksimović, Vuk, Dragišić Maksimović, Jelena, "Influence of silicon on polymerization process during lignin synthesis. Implications for cell wall properties" in International Journal of Biological Macromolecules, 198 (2022):168-174,
https://doi.org/10.1016/j.ijbiomac.2021.12.143 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1544
AB  - BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study
EP  - 2505
IS  - 6
SP  - 2500
VL  - 102
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baosic, Rada and Radotić, Ksenija",
year = "2022",
abstract = "BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study",
pages = "2505-2500",
number = "6",
volume = "102",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baosic, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baosic R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baosic, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - JOUR
AU  - Willaert, Ronnie
AU  - Vanden, Boer, Pieterjan
AU  - Malovichko, Anton
AU  - Alioscha-Perez, Mitchel
AU  - Radotić, Ksenija
AU  - Bartolić, Dragana
AU  - Kalauzi, Aleksandar
AU  - Villalba, Maria Ines
AU  - Sanglard, Dominique
AU  - Dietler, Giovanni
AU  - Sahli, Hichem
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1335
AB  - Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
PB  - Amer Assoc Advancement Science, Washington
T2  - Science Advances
T1  - Single yeast cell nanomotions correlate with cellular activity
IS  - 26
VL  - 6
DO  - 10.1126/sciadv.aba3139
ER  - 

@article{
author = "Willaert, Ronnie and Vanden, Boer, Pieterjan and Malovichko, Anton and Alioscha-Perez, Mitchel and Radotić, Ksenija and Bartolić, Dragana and Kalauzi, Aleksandar and Villalba, Maria Ines and Sanglard, Dominique and Dietler, Giovanni and Sahli, Hichem and Kasas, Sandor",
year = "2020",
abstract = "Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.",
publisher = "Amer Assoc Advancement Science, Washington",
journal = "Science Advances",
title = "Single yeast cell nanomotions correlate with cellular activity",
number = "26",
volume = "6",
doi = "10.1126/sciadv.aba3139"
}

Willaert, R., Vanden, B. P., Malovichko, A., Alioscha-Perez, M., Radotić, K., Bartolić, D., Kalauzi, A., Villalba, M. I., Sanglard, D., Dietler, G., Sahli, H.,& Kasas, S.. (2020). Single yeast cell nanomotions correlate with cellular activity. in Science Advances
Amer Assoc Advancement Science, Washington., 6(26).
https://doi.org/10.1126/sciadv.aba3139

Willaert R, Vanden BP, Malovichko A, Alioscha-Perez M, Radotić K, Bartolić D, Kalauzi A, Villalba MI, Sanglard D, Dietler G, Sahli H, Kasas S. Single yeast cell nanomotions correlate with cellular activity. in Science Advances. 2020;6(26).
doi:10.1126/sciadv.aba3139 .

Willaert, Ronnie, Vanden, Boer, Pieterjan, Malovichko, Anton, Alioscha-Perez, Mitchel, Radotić, Ksenija, Bartolić, Dragana, Kalauzi, Aleksandar, Villalba, Maria Ines, Sanglard, Dominique, Dietler, Giovanni, Sahli, Hichem, Kasas, Sandor, "Single yeast cell nanomotions correlate with cellular activity" in Science Advances, 6, no. 26 (2020),
https://doi.org/10.1126/sciadv.aba3139 . .

TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Celine
AU  - Stupar, Petar
AU  - Villalba, Maria, I
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pesic, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1338
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
PB  - Wiley, Hoboken
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
IS  - 12
VL  - 33
DO  - 10.1002/jmr.2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Celine and Stupar, Petar and Villalba, Maria, I and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pesic, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
publisher = "Wiley, Hoboken",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
number = "12",
volume = "33",
doi = "10.1002/jmr.2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pesic, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition
Wiley, Hoboken., 33(12).
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pesic M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;33(12).
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Celine, Stupar, Petar, Villalba, Maria, I, Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pesic, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition, 33, no. 12 (2020),
https://doi.org/10.1002/jmr.2849 . .

TY  - CONF
AU  - Platisa, Mirjana M.
AU  - Radovanović, N.N.
AU  - Kalauzi, Aleksandar
AU  - Milasinović, G.
AU  - Pavlović, S.U.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1306
AB  - Heart rate (HR) asymmetry is a dynamic phenomenon related to the interplay of dominant regulatory mechanisms of cardiovascular system which operate over different scales of observation. The aim of this work is to examine asymmetry phenomenon in heart failure (HF) patients with sinus rhythm. We computed Guzik's index of heart rate asymmetry related to HR deceleration (Cd) from the Poincare plot (Pp) analysis extended up to 20th order. In the control group asymmetry is maintained over all orders of the Pp. In HF patients with asymmetric properties, Cd is reduced in the range between 2nd and 5th order of the Pp analysis, compared the control subjects. More, this method revealed two clusters of HF patients. In conclusion, proposed new approach can be applied to reveal alterations in the behavior of the cardiovascular control mechanisms in pathological conditions.
PB  - Institute of Electrical and Electronics Engineers Inc.
C3  - 2020 11th Conference of the European Study Group on Cardiovascular Oscillations: Computation and Mod
T1  - Asymmetry of Cardiac Interbeat Intervals in Heart Failure
DO  - 10.1109/ESGCO49734.2020.9158154
ER  - 

@conference{
author = "Platisa, Mirjana M. and Radovanović, N.N. and Kalauzi, Aleksandar and Milasinović, G. and Pavlović, S.U.",
year = "2020",
abstract = "Heart rate (HR) asymmetry is a dynamic phenomenon related to the interplay of dominant regulatory mechanisms of cardiovascular system which operate over different scales of observation. The aim of this work is to examine asymmetry phenomenon in heart failure (HF) patients with sinus rhythm. We computed Guzik's index of heart rate asymmetry related to HR deceleration (Cd) from the Poincare plot (Pp) analysis extended up to 20th order. In the control group asymmetry is maintained over all orders of the Pp. In HF patients with asymmetric properties, Cd is reduced in the range between 2nd and 5th order of the Pp analysis, compared the control subjects. More, this method revealed two clusters of HF patients. In conclusion, proposed new approach can be applied to reveal alterations in the behavior of the cardiovascular control mechanisms in pathological conditions.",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
journal = "2020 11th Conference of the European Study Group on Cardiovascular Oscillations: Computation and Mod",
title = "Asymmetry of Cardiac Interbeat Intervals in Heart Failure",
doi = "10.1109/ESGCO49734.2020.9158154"
}

Platisa, M. M., Radovanović, N.N., Kalauzi, A., Milasinović, G.,& Pavlović, S.U.. (2020). Asymmetry of Cardiac Interbeat Intervals in Heart Failure. in 2020 11th Conference of the European Study Group on Cardiovascular Oscillations: Computation and Mod
Institute of Electrical and Electronics Engineers Inc...
https://doi.org/10.1109/ESGCO49734.2020.9158154

Platisa MM, Radovanović N, Kalauzi A, Milasinović G, Pavlović S. Asymmetry of Cardiac Interbeat Intervals in Heart Failure. in 2020 11th Conference of the European Study Group on Cardiovascular Oscillations: Computation and Mod. 2020;.
doi:10.1109/ESGCO49734.2020.9158154 .

Platisa, Mirjana M., Radovanović, N.N., Kalauzi, Aleksandar, Milasinović, G., Pavlović, S.U., "Asymmetry of Cardiac Interbeat Intervals in Heart Failure" in 2020 11th Conference of the European Study Group on Cardiovascular Oscillations: Computation and Mod (2020),
https://doi.org/10.1109/ESGCO49734.2020.9158154 . .

TY  - JOUR
AU  - Platisa, Mirjana M.
AU  - Radovanović, Nikola N.
AU  - Kalauzi, Aleksandar
AU  - Milasinović, Goran
AU  - Pavlović, Sinisa U.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1328
AB  - It is known that in pathological conditions, physiological systems develop changes in the multiscale properties of physiological signals. However, in real life, little is known about how changes in the function of one of the two coupled physiological systems induce changes in function of the other one, especially on their multiscale behavior. Hence, in this work we aimed to examine the complexity of cardio-respiratory coupled systems control using multiscale entropy (MSE) analysis of cardiac intervals MSE (RR), respiratory time series MSE (Resp), and synchrony of these rhythms by cross multiscale entropy (CMSE) analysis, in the heart failure (HF) patients and healthy subjects. We analyzed 20 min of synchronously recorded RR intervals and respiratory signal during relaxation in the supine position in 42 heart failure patients and 14 control healthy subjects. Heart failure group was divided into three subgroups, according to the RR interval time series characteristics (atrial fibrillation (HFAF), sinus rhythm (HFSin), and sinus rhythm with ventricular extrasystoles (HFVES)). Compared with healthy control subjects, alterations in respiratory signal properties were observed in patients from the HFSin and HFVES groups. Further, mean MSE curves of RR intervals and respiratory signal were not statistically different only in the HFSin group (p = 0.43). The level of synchrony between these time series was significantly higher in HFSin and HFVES patients than in control subjects and HFAF patients (p  lt  0.01). In conclusion, depending on the specific pathologies, primary alterations in the regularity of cardiac rhythm resulted in changes in the regularity of the respiratory rhythm, as well as in the level of their asynchrony.
PB  - MDPI, Basel
T2  - Entropy
T1  - Multiscale Entropy Analysis: Application to Cardio-Respiratory Coupling
IS  - 9
VL  - 22
DO  - 10.3390/e22091042
ER  - 

@article{
author = "Platisa, Mirjana M. and Radovanović, Nikola N. and Kalauzi, Aleksandar and Milasinović, Goran and Pavlović, Sinisa U.",
year = "2020",
abstract = "It is known that in pathological conditions, physiological systems develop changes in the multiscale properties of physiological signals. However, in real life, little is known about how changes in the function of one of the two coupled physiological systems induce changes in function of the other one, especially on their multiscale behavior. Hence, in this work we aimed to examine the complexity of cardio-respiratory coupled systems control using multiscale entropy (MSE) analysis of cardiac intervals MSE (RR), respiratory time series MSE (Resp), and synchrony of these rhythms by cross multiscale entropy (CMSE) analysis, in the heart failure (HF) patients and healthy subjects. We analyzed 20 min of synchronously recorded RR intervals and respiratory signal during relaxation in the supine position in 42 heart failure patients and 14 control healthy subjects. Heart failure group was divided into three subgroups, according to the RR interval time series characteristics (atrial fibrillation (HFAF), sinus rhythm (HFSin), and sinus rhythm with ventricular extrasystoles (HFVES)). Compared with healthy control subjects, alterations in respiratory signal properties were observed in patients from the HFSin and HFVES groups. Further, mean MSE curves of RR intervals and respiratory signal were not statistically different only in the HFSin group (p = 0.43). The level of synchrony between these time series was significantly higher in HFSin and HFVES patients than in control subjects and HFAF patients (p  lt  0.01). In conclusion, depending on the specific pathologies, primary alterations in the regularity of cardiac rhythm resulted in changes in the regularity of the respiratory rhythm, as well as in the level of their asynchrony.",
publisher = "MDPI, Basel",
journal = "Entropy",
title = "Multiscale Entropy Analysis: Application to Cardio-Respiratory Coupling",
number = "9",
volume = "22",
doi = "10.3390/e22091042"
}

Platisa, M. M., Radovanović, N. N., Kalauzi, A., Milasinović, G.,& Pavlović, S. U.. (2020). Multiscale Entropy Analysis: Application to Cardio-Respiratory Coupling. in Entropy
MDPI, Basel., 22(9).
https://doi.org/10.3390/e22091042

Platisa MM, Radovanović NN, Kalauzi A, Milasinović G, Pavlović SU. Multiscale Entropy Analysis: Application to Cardio-Respiratory Coupling. in Entropy. 2020;22(9).
doi:10.3390/e22091042 .

Platisa, Mirjana M., Radovanović, Nikola N., Kalauzi, Aleksandar, Milasinović, Goran, Pavlović, Sinisa U., "Multiscale Entropy Analysis: Application to Cardio-Respiratory Coupling" in Entropy, 22, no. 9 (2020),
https://doi.org/10.3390/e22091042 . .

TY  - JOUR
AU  - Matic, Zoran
AU  - Platisa, Mirjana M.
AU  - Kalauzi, Aleksandar
AU  - Bojic, Tijana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1293
AB  - Objective: We explored the physiological background of the non-linear operating mode of cardiorespiratory oscillators as the fundamental question of cardiorespiratory homeodynamics and as a prerequisite for the understanding of neurocardiovascular diseases. We investigated 20 healthy human subjects for changes using electrocardiac RR interval (RRI) and respiratory signal (Resp) Detrended Fluctuation Analysis (DFA, alpha(1RRI), alpha(2RRI), alpha(1Resp), alpha(2Resp)), Multiple Scaling Entropy (MSERRI1-4, MSERRI5-10, MSEResp1-4, MSEResp5-10), spectral coherence (Coh(RRI-Resp)), cross DFA (rho(1) and rho(2)) and cross MSE (XMSE1-4 and XMSE5-10) indices in four physiological conditions: supine with spontaneous breathing, standing with spontaneous breathing, supine with 0.1 Hz breathing and standing with 0.1 Hz breathing. Main results: Standing is primarily characterized by the change of RRI parameters, insensitivity to change with respiratory parameters, decrease of Coh(RRI-Resp) and insensitivity to change of in rho(1), rho(2), XMSE1-4, and XMSE5-10. Slow breathing in supine position was characterized by the change of the linear and non-linear parameters of both signals, reflecting the dominant vagal RRI modulation and the impact of slow 0.1 Hz breathing on Resp parameters. Coh(RRI-Resp) did not change with respect to supine position, while rho(1) increased. Slow breathing in standing reflected the qualitatively specific state of autonomic regulation with striking impact on both cardiac and respiratory parameters, with specific patterns of cardiorespiratory coupling.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Physiology
T1  - Slow 0.1 Hz Breathing and Body Posture Induced Perturbations of RRI and Respiratory Signal Complexity and Cardiorespiratory Coupling
VL  - 11
DO  - 10.3389/fphys.2020.00024
ER  - 

@article{
author = "Matic, Zoran and Platisa, Mirjana M. and Kalauzi, Aleksandar and Bojic, Tijana",
year = "2020",
abstract = "Objective: We explored the physiological background of the non-linear operating mode of cardiorespiratory oscillators as the fundamental question of cardiorespiratory homeodynamics and as a prerequisite for the understanding of neurocardiovascular diseases. We investigated 20 healthy human subjects for changes using electrocardiac RR interval (RRI) and respiratory signal (Resp) Detrended Fluctuation Analysis (DFA, alpha(1RRI), alpha(2RRI), alpha(1Resp), alpha(2Resp)), Multiple Scaling Entropy (MSERRI1-4, MSERRI5-10, MSEResp1-4, MSEResp5-10), spectral coherence (Coh(RRI-Resp)), cross DFA (rho(1) and rho(2)) and cross MSE (XMSE1-4 and XMSE5-10) indices in four physiological conditions: supine with spontaneous breathing, standing with spontaneous breathing, supine with 0.1 Hz breathing and standing with 0.1 Hz breathing. Main results: Standing is primarily characterized by the change of RRI parameters, insensitivity to change with respiratory parameters, decrease of Coh(RRI-Resp) and insensitivity to change of in rho(1), rho(2), XMSE1-4, and XMSE5-10. Slow breathing in supine position was characterized by the change of the linear and non-linear parameters of both signals, reflecting the dominant vagal RRI modulation and the impact of slow 0.1 Hz breathing on Resp parameters. Coh(RRI-Resp) did not change with respect to supine position, while rho(1) increased. Slow breathing in standing reflected the qualitatively specific state of autonomic regulation with striking impact on both cardiac and respiratory parameters, with specific patterns of cardiorespiratory coupling.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Physiology",
title = "Slow 0.1 Hz Breathing and Body Posture Induced Perturbations of RRI and Respiratory Signal Complexity and Cardiorespiratory Coupling",
volume = "11",
doi = "10.3389/fphys.2020.00024"
}

Matic, Z., Platisa, M. M., Kalauzi, A.,& Bojic, T.. (2020). Slow 0.1 Hz Breathing and Body Posture Induced Perturbations of RRI and Respiratory Signal Complexity and Cardiorespiratory Coupling. in Frontiers in Physiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fphys.2020.00024

Matic Z, Platisa MM, Kalauzi A, Bojic T. Slow 0.1 Hz Breathing and Body Posture Induced Perturbations of RRI and Respiratory Signal Complexity and Cardiorespiratory Coupling. in Frontiers in Physiology. 2020;11.
doi:10.3389/fphys.2020.00024 .

Matic, Zoran, Platisa, Mirjana M., Kalauzi, Aleksandar, Bojic, Tijana, "Slow 0.1 Hz Breathing and Body Posture Induced Perturbations of RRI and Respiratory Signal Complexity and Cardiorespiratory Coupling" in Frontiers in Physiology, 11 (2020),
https://doi.org/10.3389/fphys.2020.00024 . .

TY  - GEN
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Donaldson, Lloyd
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1654
AB  - Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.
PB  - 5th International Congress on Microscopy & Spectroscopy
T2  - 5th International Congress on Microscopy & Spectroscopy
T1  - Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1654
ER  - 

@misc{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Donaldson, Lloyd and Leblanc, Roger M. and Radotić, Ksenija",
year = "2018",
abstract = "Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.",
publisher = "5th International Congress on Microscopy & Spectroscopy",
journal = "5th International Congress on Microscopy & Spectroscopy",
title = "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1654"
}

Đikanović, D., Kalauzi, A., Donaldson, L., Leblanc, R. M.,& Radotić, K.. (2018). Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy
5th International Congress on Microscopy & Spectroscopy., 82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654

Đikanović D, Kalauzi A, Donaldson L, Leblanc RM, Radotić K. Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy. 2018;:82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

Đikanović, Daniela, Kalauzi, Aleksandar, Donaldson, Lloyd, Leblanc, Roger M., Radotić, Ksenija, "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls" in 5th International Congress on Microscopy & Spectroscopy (2018):82,
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

TY  - JOUR
AU  - Smederevac-Lalić, Marija
AU  - Kalauzi, Aleksandar
AU  - Regner, Slobodan
AU  - Navodaru, Ion
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1148
AB  - The relationship between the Lower Danube River level and Romanian annual catches of Pontic shad (Alosa immaculata, Bennett 1835) were analyzed. For analysis of long term data on the Danube River water level and Pontic shad catch, combinations of different methods were applied using statistical programs, SPSS 13.0 and MATLAB 6. Periodograms, containing cyclic patterns, were obtained using Fourier analysis. Significant oscillations were determined with Fisher-Whittle' s tests and residuals were calculated after subtracting these significant oscillations from the original signals. Autoregressive moving average (ARMA) models of residuals were finally applied. Results indicated that river water levels, and especially those in May, greatly explained the fluctuations of Pontic shad catch. Annual landings varied greatly and appeared to be cyclic. Varying river flow was considered to be one of the most important factors that cause fluctuations in the size of populations. Forecast indicates gradual increase of the catch in the next decade, followed by a decrease in other decades. Estimated as a vulnerable species of fish by the IUCN, development of the forecasting model of the future catch oscillations could be very helpful to regulate fishing efforts towards the sustainable use of stocks and species conservation.
PB  - Iranian Fisheries Science Research Inst-Ifsri, Tehran
T2  - Iranian Journal of Fisheries Sciences
T1  - Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River
EP  - 457
IS  - 3
SP  - 443
VL  - 17
DO  - 10.22092/IJFS.2018.116611
ER  - 

@article{
author = "Smederevac-Lalić, Marija and Kalauzi, Aleksandar and Regner, Slobodan and Navodaru, Ion and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana",
year = "2018",
abstract = "The relationship between the Lower Danube River level and Romanian annual catches of Pontic shad (Alosa immaculata, Bennett 1835) were analyzed. For analysis of long term data on the Danube River water level and Pontic shad catch, combinations of different methods were applied using statistical programs, SPSS 13.0 and MATLAB 6. Periodograms, containing cyclic patterns, were obtained using Fourier analysis. Significant oscillations were determined with Fisher-Whittle' s tests and residuals were calculated after subtracting these significant oscillations from the original signals. Autoregressive moving average (ARMA) models of residuals were finally applied. Results indicated that river water levels, and especially those in May, greatly explained the fluctuations of Pontic shad catch. Annual landings varied greatly and appeared to be cyclic. Varying river flow was considered to be one of the most important factors that cause fluctuations in the size of populations. Forecast indicates gradual increase of the catch in the next decade, followed by a decrease in other decades. Estimated as a vulnerable species of fish by the IUCN, development of the forecasting model of the future catch oscillations could be very helpful to regulate fishing efforts towards the sustainable use of stocks and species conservation.",
publisher = "Iranian Fisheries Science Research Inst-Ifsri, Tehran",
journal = "Iranian Journal of Fisheries Sciences",
title = "Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River",
pages = "457-443",
number = "3",
volume = "17",
doi = "10.22092/IJFS.2018.116611"
}

Smederevac-Lalić, M., Kalauzi, A., Regner, S., Navodaru, I., Višnjić Jeftić, Ž., Gačić, Z.,& Lenhardt, M.. (2018). Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River. in Iranian Journal of Fisheries Sciences
Iranian Fisheries Science Research Inst-Ifsri, Tehran., 17(3), 443-457.
https://doi.org/10.22092/IJFS.2018.116611

Smederevac-Lalić M, Kalauzi A, Regner S, Navodaru I, Višnjić Jeftić Ž, Gačić Z, Lenhardt M. Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River. in Iranian Journal of Fisheries Sciences. 2018;17(3):443-457.
doi:10.22092/IJFS.2018.116611 .

Smederevac-Lalić, Marija, Kalauzi, Aleksandar, Regner, Slobodan, Navodaru, Ion, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, "Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River" in Iranian Journal of Fisheries Sciences, 17, no. 3 (2018):443-457,
https://doi.org/10.22092/IJFS.2018.116611 . .

TY  - JOUR
AU  - Stupar, Petar
AU  - Chomicki, Wojciech
AU  - Maillard, Caroline
AU  - Mikeladze, David
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Dietler, Giovanni
AU  - Kasas, Sandor
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1037
AB  - Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.
PB  - Copernicus Gesellschaft Mbh, Gottingen
T2  - Mechanical Sciences
T1  - Mitochondrial activity detected by cantilever based sensor
EP  - 28
IS  - 1
SP  - 23
VL  - 8
DO  - 10.5194/ms-8-23-2017
ER  - 

@article{
author = "Stupar, Petar and Chomicki, Wojciech and Maillard, Caroline and Mikeladze, David and Kalauzi, Aleksandar and Radotić, Ksenija and Dietler, Giovanni and Kasas, Sandor",
year = "2017",
abstract = "Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.",
publisher = "Copernicus Gesellschaft Mbh, Gottingen",
journal = "Mechanical Sciences",
title = "Mitochondrial activity detected by cantilever based sensor",
pages = "28-23",
number = "1",
volume = "8",
doi = "10.5194/ms-8-23-2017"
}

Stupar, P., Chomicki, W., Maillard, C., Mikeladze, D., Kalauzi, A., Radotić, K., Dietler, G.,& Kasas, S.. (2017). Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences
Copernicus Gesellschaft Mbh, Gottingen., 8(1), 23-28.
https://doi.org/10.5194/ms-8-23-2017

Stupar P, Chomicki W, Maillard C, Mikeladze D, Kalauzi A, Radotić K, Dietler G, Kasas S. Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences. 2017;8(1):23-28.
doi:10.5194/ms-8-23-2017 .

Stupar, Petar, Chomicki, Wojciech, Maillard, Caroline, Mikeladze, David, Kalauzi, Aleksandar, Radotić, Ksenija, Dietler, Giovanni, Kasas, Sandor, "Mitochondrial activity detected by cantilever based sensor" in Mechanical Sciences, 8, no. 1 (2017):23-28,
https://doi.org/10.5194/ms-8-23-2017 . .

TY  - JOUR
AU  - Smederevac-Lalić, Marija
AU  - Kalauzi, Aleksandar
AU  - Regner, Slobodan
AU  - Lenhardt, Mirjana
AU  - Naunović, Zorana
AU  - Hegediš, Aleksandar
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1042
AB  - The effects of physical factors on fish catch in the Serbian part of the Danube River were studied for period of six decades. The data on total catch for the Danube River from river kilometre 845 to river kilometre 1433 were collected from Statistical Office of the Republic of Serbia, while water level and water temperature data were collected from 16 water gauge stations along the investigated part of the Danube River for the period 1948-2009. Cross-correlation functions have been used to analyse the functional connection between Danube water level, water temperature and fish catch while ARMA model which combines cyclic (deterministic) and random (stochastic) components of the analysed sequences was used for the forecasts. The cross-correlation function showed negative correlation between water level and temperature as well as between water temperature and catch and positive correlation between water level and catch. The Danube water level and catch were coherent at the periods of 2.06, 4.13, 6.2, 10.33, 20.66 years, while the cross correlation function between these time series did not show phase lag. The results of reconstruction and forecast of water level, temperature, and catch of fish in the Danube River, obtained by summing the cyclic and stochastic components, was used for the forecast till 2029. In 2016, seven years after, the initial forecasts were made, validity of the model was checked by obtaining data for water temperature, water level and fish catch in the Danube River for the period 2010-2015. Model gave the best prediction for water temperature; average standard error was 1.6 times higher for predicted value than for model value while for fish catch and water level they were 1.96 and 4.97, respectively. Methods used in this work could be powerful tool for prediction of fish catch and serve as the basis for better fisheries management.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Prediction of fish catch in the Danube River based on long-term variability in environmental parameters and catch statistics
EP  - 671
SP  - 664
VL  - 609
DO  - 10.1016/j.scitotenv.2017.07.177
ER  - 

@article{
author = "Smederevac-Lalić, Marija and Kalauzi, Aleksandar and Regner, Slobodan and Lenhardt, Mirjana and Naunović, Zorana and Hegediš, Aleksandar",
year = "2017",
abstract = "The effects of physical factors on fish catch in the Serbian part of the Danube River were studied for period of six decades. The data on total catch for the Danube River from river kilometre 845 to river kilometre 1433 were collected from Statistical Office of the Republic of Serbia, while water level and water temperature data were collected from 16 water gauge stations along the investigated part of the Danube River for the period 1948-2009. Cross-correlation functions have been used to analyse the functional connection between Danube water level, water temperature and fish catch while ARMA model which combines cyclic (deterministic) and random (stochastic) components of the analysed sequences was used for the forecasts. The cross-correlation function showed negative correlation between water level and temperature as well as between water temperature and catch and positive correlation between water level and catch. The Danube water level and catch were coherent at the periods of 2.06, 4.13, 6.2, 10.33, 20.66 years, while the cross correlation function between these time series did not show phase lag. The results of reconstruction and forecast of water level, temperature, and catch of fish in the Danube River, obtained by summing the cyclic and stochastic components, was used for the forecast till 2029. In 2016, seven years after, the initial forecasts were made, validity of the model was checked by obtaining data for water temperature, water level and fish catch in the Danube River for the period 2010-2015. Model gave the best prediction for water temperature; average standard error was 1.6 times higher for predicted value than for model value while for fish catch and water level they were 1.96 and 4.97, respectively. Methods used in this work could be powerful tool for prediction of fish catch and serve as the basis for better fisheries management.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Prediction of fish catch in the Danube River based on long-term variability in environmental parameters and catch statistics",
pages = "671-664",
volume = "609",
doi = "10.1016/j.scitotenv.2017.07.177"
}

Smederevac-Lalić, M., Kalauzi, A., Regner, S., Lenhardt, M., Naunović, Z.,& Hegediš, A.. (2017). Prediction of fish catch in the Danube River based on long-term variability in environmental parameters and catch statistics. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 609, 664-671.
https://doi.org/10.1016/j.scitotenv.2017.07.177

Smederevac-Lalić M, Kalauzi A, Regner S, Lenhardt M, Naunović Z, Hegediš A. Prediction of fish catch in the Danube River based on long-term variability in environmental parameters and catch statistics. in Science of the Total Environment. 2017;609:664-671.
doi:10.1016/j.scitotenv.2017.07.177 .

Smederevac-Lalić, Marija, Kalauzi, Aleksandar, Regner, Slobodan, Lenhardt, Mirjana, Naunović, Zorana, Hegediš, Aleksandar, "Prediction of fish catch in the Danube River based on long-term variability in environmental parameters and catch statistics" in Science of the Total Environment, 609 (2017):664-671,
https://doi.org/10.1016/j.scitotenv.2017.07.177 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Popovic-Bijelic, Ana
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Kalauzi, Aleksandar
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/950
AB  - Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.
PB  - Elsevier, Amsterdam
T2  - Applied Catalysis B-Environmental
T1  - Photo-redox reactions of indole and ferric iron in water
EP  - 180
SP  - 174
VL  - 185
DO  - 10.1016/j.apcatb.2015.12.018
ER  - 

@article{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Popovic-Bijelic, Ana and Zakrzewska, Joanna and Stanić, Marina and Kalauzi, Aleksandar and Spasojević, Ivan",
year = "2016",
abstract = "Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Catalysis B-Environmental",
title = "Photo-redox reactions of indole and ferric iron in water",
pages = "180-174",
volume = "185",
doi = "10.1016/j.apcatb.2015.12.018"
}

Milić Komić, S., Bogdanović Pristov, J., Popovic-Bijelic, A., Zakrzewska, J., Stanić, M., Kalauzi, A.,& Spasojević, I.. (2016). Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental
Elsevier, Amsterdam., 185, 174-180.
https://doi.org/10.1016/j.apcatb.2015.12.018

Milić Komić S, Bogdanović Pristov J, Popovic-Bijelic A, Zakrzewska J, Stanić M, Kalauzi A, Spasojević I. Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental. 2016;185:174-180.
doi:10.1016/j.apcatb.2015.12.018 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Popovic-Bijelic, Ana, Zakrzewska, Joanna, Stanić, Marina, Kalauzi, Aleksandar, Spasojević, Ivan, "Photo-redox reactions of indole and ferric iron in water" in Applied Catalysis B-Environmental, 185 (2016):174-180,
https://doi.org/10.1016/j.apcatb.2015.12.018 . .

TY  - JOUR
AU  - Jarić, Ivan
AU  - Smederevac-Lalić, Marija
AU  - Jovicic, Katarina
AU  - Jaćimović, Milica
AU  - Cvijanović, Gorčin
AU  - Lenhardt, Mirjana
AU  - Kalauzi, Aleksandar
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/985
AB  - Fishery in the Danube River basin has been characterised over the past century by increasing fishing levels, illegal fishing practices and poor regulations. However, there is a remarkable lack of available information on the actual status of fish stocks, as well as on the trends and sustainability of fisheries, which poses a problem for the development of adequate policy and management measures. In this study, we assessed the trends in the commercial fishery in the Middle Danube in Serbia during 1969-1989 and 2006-2010 by evaluating the temporal changes in life history-related indicators that might point out unsustainable fishing pressures. Moreover, we present the approach of using the catch-weighted mean egg-per-recruit (EPR) index as a proxy for the overall resilience of fish stocks to fishing. Results indicated a marked shift towards smaller fish that mature earlier and have a shorter lifespan. Landings also shifted towards species at lower trophic levels, with a mean trophic level decline at a rate of approximately 0.16 per decade. Results indicated likely presence of the fishing through the food web' phenomenon. At the same time, catch-weighted community mean of the 20% EPR threshold ratio (EPR20%) increased by 4.2%, indicating the increase of the overall resilience to fishing of the exploited species. Obtained results indicated the importance of using such metrics for the assessments of trends in fishery. The approach and results presented here could be of interest for the scientific community and stakeholders involved in fishery management.
PB  - Wiley, Hoboken
T2  - Ecology of Freshwater Fish
T1  - Indicators of unsustainable fishery in the Middle Danube
EP  - 98
IS  - 1
SP  - 86
VL  - 25
DO  - 10.1111/eff.12193
ER  - 

@article{
author = "Jarić, Ivan and Smederevac-Lalić, Marija and Jovicic, Katarina and Jaćimović, Milica and Cvijanović, Gorčin and Lenhardt, Mirjana and Kalauzi, Aleksandar",
year = "2016",
abstract = "Fishery in the Danube River basin has been characterised over the past century by increasing fishing levels, illegal fishing practices and poor regulations. However, there is a remarkable lack of available information on the actual status of fish stocks, as well as on the trends and sustainability of fisheries, which poses a problem for the development of adequate policy and management measures. In this study, we assessed the trends in the commercial fishery in the Middle Danube in Serbia during 1969-1989 and 2006-2010 by evaluating the temporal changes in life history-related indicators that might point out unsustainable fishing pressures. Moreover, we present the approach of using the catch-weighted mean egg-per-recruit (EPR) index as a proxy for the overall resilience of fish stocks to fishing. Results indicated a marked shift towards smaller fish that mature earlier and have a shorter lifespan. Landings also shifted towards species at lower trophic levels, with a mean trophic level decline at a rate of approximately 0.16 per decade. Results indicated likely presence of the fishing through the food web' phenomenon. At the same time, catch-weighted community mean of the 20% EPR threshold ratio (EPR20%) increased by 4.2%, indicating the increase of the overall resilience to fishing of the exploited species. Obtained results indicated the importance of using such metrics for the assessments of trends in fishery. The approach and results presented here could be of interest for the scientific community and stakeholders involved in fishery management.",
publisher = "Wiley, Hoboken",
journal = "Ecology of Freshwater Fish",
title = "Indicators of unsustainable fishery in the Middle Danube",
pages = "98-86",
number = "1",
volume = "25",
doi = "10.1111/eff.12193"
}

Jarić, I., Smederevac-Lalić, M., Jovicic, K., Jaćimović, M., Cvijanović, G., Lenhardt, M.,& Kalauzi, A.. (2016). Indicators of unsustainable fishery in the Middle Danube. in Ecology of Freshwater Fish
Wiley, Hoboken., 25(1), 86-98.
https://doi.org/10.1111/eff.12193

Jarić I, Smederevac-Lalić M, Jovicic K, Jaćimović M, Cvijanović G, Lenhardt M, Kalauzi A. Indicators of unsustainable fishery in the Middle Danube. in Ecology of Freshwater Fish. 2016;25(1):86-98.
doi:10.1111/eff.12193 .

Jarić, Ivan, Smederevac-Lalić, Marija, Jovicic, Katarina, Jaćimović, Milica, Cvijanović, Gorčin, Lenhardt, Mirjana, Kalauzi, Aleksandar, "Indicators of unsustainable fishery in the Middle Danube" in Ecology of Freshwater Fish, 25, no. 1 (2016):86-98,
https://doi.org/10.1111/eff.12193 . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Navodaru, Ion
AU  - Vassilev, M.
AU  - Kalauzi, Aleksandar
AU  - Regner, Slobodan
AU  - Višnjić Jeftić, Željka
AU  - Tošić, K.
AU  - Smederevac-Lalić, Marija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/961
AB  - Data on the catch of Pontic shad in the Lower Danube River Region for the period 1920-2008 were used to make one-component and two-component models. The catch model for Pontic shad showed natural cyclic fluctuations with no sign of population decrease. The oscillation periods were 11.17 years and 9.56 years, which corresponded to solar activity cycles. In addition, as anchovy represents the main food source of Pontic shad in the Black Sea, the catch of anchovy in the Black Sea for the period 1950-2006 was modelled. Pearson's coefficient (cc=0.6785) indicated that 67.86% of the Pontic shad catch was dependent on the anchovy catch during the analysed time period. Besides the necessity for better gathering of data in the Lower Danube River Region on the catch of Pontic shad, there is also a need for more profound studies on this species. It is evident that this valuable fish species requires more attention from fish managers in all countries of the Lower Danube River Region.
PB  - Bulgarian Academy of Sciences
T2  - Acta Zoologica Bulgarica
T1  - Model of the Pontic Shad Alosa immaculata (Bennet, 1835) and Anchovy Engraulis encrasicolus (Linnaeus, 1758) Catch in the Danube River and Black Sea for the Period 1920-2008
EP  - 561
IS  - 4
SP  - 557
VL  - 68
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_961
ER  - 

@article{
author = "Lenhardt, Mirjana and Navodaru, Ion and Vassilev, M. and Kalauzi, Aleksandar and Regner, Slobodan and Višnjić Jeftić, Željka and Tošić, K. and Smederevac-Lalić, Marija",
year = "2016",
abstract = "Data on the catch of Pontic shad in the Lower Danube River Region for the period 1920-2008 were used to make one-component and two-component models. The catch model for Pontic shad showed natural cyclic fluctuations with no sign of population decrease. The oscillation periods were 11.17 years and 9.56 years, which corresponded to solar activity cycles. In addition, as anchovy represents the main food source of Pontic shad in the Black Sea, the catch of anchovy in the Black Sea for the period 1950-2006 was modelled. Pearson's coefficient (cc=0.6785) indicated that 67.86% of the Pontic shad catch was dependent on the anchovy catch during the analysed time period. Besides the necessity for better gathering of data in the Lower Danube River Region on the catch of Pontic shad, there is also a need for more profound studies on this species. It is evident that this valuable fish species requires more attention from fish managers in all countries of the Lower Danube River Region.",
publisher = "Bulgarian Academy of Sciences",
journal = "Acta Zoologica Bulgarica",
title = "Model of the Pontic Shad Alosa immaculata (Bennet, 1835) and Anchovy Engraulis encrasicolus (Linnaeus, 1758) Catch in the Danube River and Black Sea for the Period 1920-2008",
pages = "561-557",
number = "4",
volume = "68",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_961"
}

Lenhardt, M., Navodaru, I., Vassilev, M., Kalauzi, A., Regner, S., Višnjić Jeftić, Ž., Tošić, K.,& Smederevac-Lalić, M.. (2016). Model of the Pontic Shad Alosa immaculata (Bennet, 1835) and Anchovy Engraulis encrasicolus (Linnaeus, 1758) Catch in the Danube River and Black Sea for the Period 1920-2008. in Acta Zoologica Bulgarica
Bulgarian Academy of Sciences., 68(4), 557-561.
https://hdl.handle.net/21.15107/rcub_rimsi_961

Lenhardt M, Navodaru I, Vassilev M, Kalauzi A, Regner S, Višnjić Jeftić Ž, Tošić K, Smederevac-Lalić M. Model of the Pontic Shad Alosa immaculata (Bennet, 1835) and Anchovy Engraulis encrasicolus (Linnaeus, 1758) Catch in the Danube River and Black Sea for the Period 1920-2008. in Acta Zoologica Bulgarica. 2016;68(4):557-561.
https://hdl.handle.net/21.15107/rcub_rimsi_961 .

Lenhardt, Mirjana, Navodaru, Ion, Vassilev, M., Kalauzi, Aleksandar, Regner, Slobodan, Višnjić Jeftić, Željka, Tošić, K., Smederevac-Lalić, Marija, "Model of the Pontic Shad Alosa immaculata (Bennet, 1835) and Anchovy Engraulis encrasicolus (Linnaeus, 1758) Catch in the Danube River and Black Sea for the Period 1920-2008" in Acta Zoologica Bulgarica, 68, no. 4 (2016):557-561,
https://hdl.handle.net/21.15107/rcub_rimsi_961 .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Devečerski, Aleksandar B
AU  - Steinbach, Gabor
AU  - Simonović Radosavljević, Jasna
AU  - Matović, Branko
AU  - Garab, Gyozo
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1021
AB  - Interactions between macromolecules in the cell walls of different plant origin were compared, namely spruce wood (Picea omorika (PaniA double dagger) PurkiAe) as an example of softwood, maple wood (Acer platanoides L.) as a hardwood and maize stems (Zea mays L.) as a herbaceous plant from the grass family and widely used agricultural plant. Interactions of macromolecules in isolated cell walls from the three species were compared by using Fourier transform infrared spectroscopy, X-ray diffraction and fluorescence spectroscopy. Linear dichroism of the cell walls was observed by using differential polarization laser scanning microscope (DP-LSM), which provides information of macromolecular order. This method has not previously been used for comparison of the cell walls of various plant origins. It was shown that the maize cell walls have higher amount of hydrogen bonds that lead to more regular packing of cellulose molecules, simpler structure of lignin, and a higher crystallinity of the cell wall in relation to the walls of woody plants. DP-LSM and fluorescence spectroscopy results indicate that maize has simpler and more ordered structure than both woody species. The results of this work provide new data for comparison of the cell wall properties that may be important for selection of appropriate plant for possible applications as a source of biomass. This may be a contribution to the development of efficient deconstruction and separation technologies that enable release of sugar and aromatic compounds from the cell wall macromolecular structure.
PB  - Springer, New York
T2  - Wood Science and Technology
T1  - Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction
EP  - 566
IS  - 3
SP  - 547
VL  - 50
DO  - 10.1007/s00226-015-0792-y
ER  - 

@article{
author = "Đikanović, Daniela and Devečerski, Aleksandar B and Steinbach, Gabor and Simonović Radosavljević, Jasna and Matović, Branko and Garab, Gyozo and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2016",
abstract = "Interactions between macromolecules in the cell walls of different plant origin were compared, namely spruce wood (Picea omorika (PaniA double dagger) PurkiAe) as an example of softwood, maple wood (Acer platanoides L.) as a hardwood and maize stems (Zea mays L.) as a herbaceous plant from the grass family and widely used agricultural plant. Interactions of macromolecules in isolated cell walls from the three species were compared by using Fourier transform infrared spectroscopy, X-ray diffraction and fluorescence spectroscopy. Linear dichroism of the cell walls was observed by using differential polarization laser scanning microscope (DP-LSM), which provides information of macromolecular order. This method has not previously been used for comparison of the cell walls of various plant origins. It was shown that the maize cell walls have higher amount of hydrogen bonds that lead to more regular packing of cellulose molecules, simpler structure of lignin, and a higher crystallinity of the cell wall in relation to the walls of woody plants. DP-LSM and fluorescence spectroscopy results indicate that maize has simpler and more ordered structure than both woody species. The results of this work provide new data for comparison of the cell wall properties that may be important for selection of appropriate plant for possible applications as a source of biomass. This may be a contribution to the development of efficient deconstruction and separation technologies that enable release of sugar and aromatic compounds from the cell wall macromolecular structure.",
publisher = "Springer, New York",
journal = "Wood Science and Technology",
title = "Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction",
pages = "566-547",
number = "3",
volume = "50",
doi = "10.1007/s00226-015-0792-y"
}

Đikanović, D., Devečerski, A. B., Steinbach, G., Simonović Radosavljević, J., Matović, B., Garab, G., Kalauzi, A.,& Radotić, K.. (2016). Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction. in Wood Science and Technology
Springer, New York., 50(3), 547-566.
https://doi.org/10.1007/s00226-015-0792-y

Đikanović D, Devečerski AB, Steinbach G, Simonović Radosavljević J, Matović B, Garab G, Kalauzi A, Radotić K. Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction. in Wood Science and Technology. 2016;50(3):547-566.
doi:10.1007/s00226-015-0792-y .

Đikanović, Daniela, Devečerski, Aleksandar B, Steinbach, Gabor, Simonović Radosavljević, Jasna, Matović, Branko, Garab, Gyozo, Kalauzi, Aleksandar, Radotić, Ksenija, "Comparison of macromolecular interactions in the cell walls of hardwood, softwood and maize by fluorescence and FTIR spectroscopy, differential polarization laser scanning microscopy and X-ray diffraction" in Wood Science and Technology, 50, no. 3 (2016):547-566,
https://doi.org/10.1007/s00226-015-0792-y . .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Donaldson, Lloyd
AU  - Đikanović, Daniela
AU  - Bogdanović Pristov, Jelena
AU  - Simonović Radosavljević, Jasna
AU  - Mutavdžić, Dragosav
AU  - Kalauzi, Aleksandar
AU  - Maksimović, Vuk
AU  - Nanayakkara, Bernadette
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/880
AB  - Young P. omorika trees subjected to static bending showed a severe compression wood response as characterized by fluorescence spectroscopy/microscopy, which decreased in severity with height correlated with a decrease in bending moment. This investigation is aimed at understanding the reaction wood response in a slow-growing conifer species under conditions of severe and long-term bending stress. Compression wood (CW) formation was studied in stems of juvenile P. omorika after trees were subjected to static bending by wiring at an angle of about 90 degrees, for 1 year. The applied static bending would correspond to the impact of winter snow loads or snow falls on juvenile conifers. Stem sections were collected during one growing season and examined by fluorescence microscopy, and fluorescence spectroscopy including deconvolution analysis. Trees exposed to bending produced large amounts of severe CW but very low amounts of opposite wood (OW) during the experimental season indicating a dramatic change in biomass distribution compared to control trees. Indicators of cell wall structure changes, such as fluorescence emission spectra, peak intensities, and shifts in the positions of the long-wavelength spectral components, decreased from the stem base to the top of the stem, in line with a calculated decrease in bending moment.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne
EP  - 1543
IS  - 5
SP  - 1533
VL  - 29
DO  - 10.1007/s00468-015-1234-z
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Donaldson, Lloyd and Đikanović, Daniela and Bogdanović Pristov, Jelena and Simonović Radosavljević, Jasna and Mutavdžić, Dragosav and Kalauzi, Aleksandar and Maksimović, Vuk and Nanayakkara, Bernadette and Radotić, Ksenija",
year = "2015",
abstract = "Young P. omorika trees subjected to static bending showed a severe compression wood response as characterized by fluorescence spectroscopy/microscopy, which decreased in severity with height correlated with a decrease in bending moment. This investigation is aimed at understanding the reaction wood response in a slow-growing conifer species under conditions of severe and long-term bending stress. Compression wood (CW) formation was studied in stems of juvenile P. omorika after trees were subjected to static bending by wiring at an angle of about 90 degrees, for 1 year. The applied static bending would correspond to the impact of winter snow loads or snow falls on juvenile conifers. Stem sections were collected during one growing season and examined by fluorescence microscopy, and fluorescence spectroscopy including deconvolution analysis. Trees exposed to bending produced large amounts of severe CW but very low amounts of opposite wood (OW) during the experimental season indicating a dramatic change in biomass distribution compared to control trees. Indicators of cell wall structure changes, such as fluorescence emission spectra, peak intensities, and shifts in the positions of the long-wavelength spectral components, decreased from the stem base to the top of the stem, in line with a calculated decrease in bending moment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne",
pages = "1543-1533",
number = "5",
volume = "29",
doi = "10.1007/s00468-015-1234-z"
}

Mitrović, A. Lj., Donaldson, L., Đikanović, D., Bogdanović Pristov, J., Simonović Radosavljević, J., Mutavdžić, D., Kalauzi, A., Maksimović, V., Nanayakkara, B.,& Radotić, K.. (2015). Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 29(5), 1533-1543.
https://doi.org/10.1007/s00468-015-1234-z

Mitrović AL, Donaldson L, Đikanović D, Bogdanović Pristov J, Simonović Radosavljević J, Mutavdžić D, Kalauzi A, Maksimović V, Nanayakkara B, Radotić K. Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne. in Trees-Structure and Function. 2015;29(5):1533-1543.
doi:10.1007/s00468-015-1234-z .

Mitrović, Aleksandra Lj., Donaldson, Lloyd, Đikanović, Daniela, Bogdanović Pristov, Jelena, Simonović Radosavljević, Jasna, Mutavdžić, Dragosav, Kalauzi, Aleksandar, Maksimović, Vuk, Nanayakkara, Bernadette, Radotić, Ksenija, "Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne" in Trees-Structure and Function, 29, no. 5 (2015):1533-1543,
https://doi.org/10.1007/s00468-015-1234-z . .

TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Nanayakkara, Bernadette
AU  - Radotić, Ksenija
AU  - Đikanović, Daniela
AU  - Mitrović, Aleksandra Lj.
AU  - Bogdanović Pristov, Jelena
AU  - Simonović Radosavljević, Jasna
AU  - Kalauzi, Aleksandar
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/844
AB  - Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls. Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants. Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine). It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall. A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers. Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids. Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours. Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.
PB  - Springer, New York
T2  - Planta
T1  - Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood
EP  - 1424
IS  - 6
SP  - 1413
VL  - 242
DO  - 10.1007/s00425-015-2381-6
ER  - 

@article{
author = "Donaldson, Lloyd and Nanayakkara, Bernadette and Radotić, Ksenija and Đikanović, Daniela and Mitrović, Aleksandra Lj. and Bogdanović Pristov, Jelena and Simonović Radosavljević, Jasna and Kalauzi, Aleksandar",
year = "2015",
abstract = "Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls. Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants. Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine). It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall. A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers. Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids. Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours. Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.",
publisher = "Springer, New York",
journal = "Planta",
title = "Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood",
pages = "1424-1413",
number = "6",
volume = "242",
doi = "10.1007/s00425-015-2381-6"
}

Donaldson, L., Nanayakkara, B., Radotić, K., Đikanović, D., Mitrović, A. Lj., Bogdanović Pristov, J., Simonović Radosavljević, J.,& Kalauzi, A.. (2015). Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood. in Planta
Springer, New York., 242(6), 1413-1424.
https://doi.org/10.1007/s00425-015-2381-6

Donaldson L, Nanayakkara B, Radotić K, Đikanović D, Mitrović AL, Bogdanović Pristov J, Simonović Radosavljević J, Kalauzi A. Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood. in Planta. 2015;242(6):1413-1424.
doi:10.1007/s00425-015-2381-6 .

Donaldson, Lloyd, Nanayakkara, Bernadette, Radotić, Ksenija, Đikanović, Daniela, Mitrović, Aleksandra Lj., Bogdanović Pristov, Jelena, Simonović Radosavljević, Jasna, Kalauzi, Aleksandar, "Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood" in Planta, 242, no. 6 (2015):1413-1424,
https://doi.org/10.1007/s00425-015-2381-6 . .

TY  - JOUR
AU  - Pesic, Milica
AU  - Podolski-Renic, Ana
AU  - Stojković, Sonja
AU  - Matović, Branko
AU  - Zmejkoski, Danica
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Pavicević, Aleksandra
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
AU  - Milenković, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/931
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity
EP  - 93
SP  - 85
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
ER  - 

@article{
author = "Pesic, Milica and Podolski-Renic, Ana and Stojković, Sonja and Matović, Branko and Zmejkoski, Danica and Kojić, Vesna and Bogdanović, Gordana and Pavicević, Aleksandra and Mojović, Miloš and Savić, Aleksandar G and Milenković, Ivana and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity",
pages = "93-85",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013"
}

Pesic, M., Podolski-Renic, A., Stojković, S., Matović, B., Zmejkoski, D., Kojić, V., Bogdanović, G., Pavicević, A., Mojović, M., Savić, A. G., Milenković, I., Kalauzi, A.,& Radotić, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pesic M, Podolski-Renic A, Stojković S, Matović B, Zmejkoski D, Kojić V, Bogdanović G, Pavicević A, Mojović M, Savić AG, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pesic, Milica, Podolski-Renic, Ana, Stojković, Sonja, Matović, Branko, Zmejkoski, Danica, Kojić, Vesna, Bogdanović, Gordana, Pavicević, Aleksandra, Mojović, Miloš, Savić, Aleksandar G, Milenković, Ivana, Kalauzi, Aleksandar, Radotić, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .

TY  - JOUR
AU  - Kalauzi, Aleksandar
AU  - Nikolić, Ljiljana M.
AU  - Savić, Danijela Z
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/897
AB  - The physiological and biochemical factors that lead to cell death have not been recognized completely. To our knowledge, there are no data on the bioelectric parameters that characterize early period of cell death, as well as on the appearance of related membrane current frequencies. We studied early parameters of glutaraldehyde (GA)-induced cell death, by examining the membrane properties of mouse microglia using the whole-cell patch-clamp technique. In addition, we investigated the GA-induced changes in the membrane current frequency, to see if characteristic frequencies would appear in dying cell. For data analysis, we applied a new approach, an improved multiple moving window length analysis and interval weighted spectra averaging (IWSA). We chose GA for its ability to induce almost instantaneous cell death. The 0.6 % GA did not induce changes in the bioelectric membrane properties of microglia. However, the 3 % GA caused significant decrease of membrane capacitance and resistance accompanied by the prominent increase in the membrane currents and nearly ohmic current response of microglial cells. These data indicate that 3 % GA caused complete loss of the membrane function consequently inducing instantaneous cell death. The membrane function loss was characterized by appearance of the 1.26-4.62 Hz frequency peak in the IWSA spectra, while no significant increase of amplitudes could be observed for cells treated with 0.6 % GA. To our knowledge, this is the first record of a frequency associated with complete loss of the membrane function and thus can be considered as an early indicator of cell death.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde
EP  - 123
IS  - 1
SP  - 117
VL  - 248
DO  - 10.1007/s00232-014-9748-7
ER  - 

@article{
author = "Kalauzi, Aleksandar and Nikolić, Ljiljana M. and Savić, Danijela Z and Radotić, Ksenija",
year = "2015",
abstract = "The physiological and biochemical factors that lead to cell death have not been recognized completely. To our knowledge, there are no data on the bioelectric parameters that characterize early period of cell death, as well as on the appearance of related membrane current frequencies. We studied early parameters of glutaraldehyde (GA)-induced cell death, by examining the membrane properties of mouse microglia using the whole-cell patch-clamp technique. In addition, we investigated the GA-induced changes in the membrane current frequency, to see if characteristic frequencies would appear in dying cell. For data analysis, we applied a new approach, an improved multiple moving window length analysis and interval weighted spectra averaging (IWSA). We chose GA for its ability to induce almost instantaneous cell death. The 0.6 % GA did not induce changes in the bioelectric membrane properties of microglia. However, the 3 % GA caused significant decrease of membrane capacitance and resistance accompanied by the prominent increase in the membrane currents and nearly ohmic current response of microglial cells. These data indicate that 3 % GA caused complete loss of the membrane function consequently inducing instantaneous cell death. The membrane function loss was characterized by appearance of the 1.26-4.62 Hz frequency peak in the IWSA spectra, while no significant increase of amplitudes could be observed for cells treated with 0.6 % GA. To our knowledge, this is the first record of a frequency associated with complete loss of the membrane function and thus can be considered as an early indicator of cell death.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde",
pages = "123-117",
number = "1",
volume = "248",
doi = "10.1007/s00232-014-9748-7"
}

Kalauzi, A., Nikolić, L. M., Savić, D. Z.,& Radotić, K.. (2015). Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology
Springer, New York., 248(1), 117-123.
https://doi.org/10.1007/s00232-014-9748-7

Kalauzi A, Nikolić LM, Savić DZ, Radotić K. Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology. 2015;248(1):117-123.
doi:10.1007/s00232-014-9748-7 .

Kalauzi, Aleksandar, Nikolić, Ljiljana M., Savić, Danijela Z, Radotić, Ksenija, "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde" in Journal of Membrane Biology, 248, no. 1 (2015):117-123,
https://doi.org/10.1007/s00232-014-9748-7 . .

TY  - JOUR
AU  - Algarra, Manuel
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Savić, Aleksandar G
AU  - Jimenez-Jimenez, Jose
AU  - Rodriguez-Castellon, Enrique
AU  - Esteves da Silva, Joaquim C.G.
AU  - Jose Guerrero-Gonzalez, Juan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/794
AB  - A fluorescent nanocomposite based on the inclusion of CdSe quantum dots in porous phosphate heterostructures, functionalized with amino groups (PPH-NH2"CdSe), was synthesized, characterized and used for fingerprint detection. The main scopes of this work were first to develop a friendly chemical powder for detecting latent fingerprints, especially in non-porous surfaces; their further intercalation in PPH structure enables not to spread the fluorescent nanoparticles, for that reason very good fluorescent images can be obtained. The fingerprints, obtained on different non-porous surfaces such as iron tweezers, mobile telephone screen and magnetic band of a credit card, treated with this powder emit a pale orange luminescence under ultraviolet excitation. A further image processing consists of contrast enhancement that allows obtaining positive matches according to the information supplied from a police database, and showed to be more effective than that obtained with the non-processed images. Experimental results illustrate the effectiveness of proposed methods.
PB  - Elsevier, Amsterdam
T2  - Analytica Chimica Acta
T1  - Fingerprint detection and using intercalated CdSe nanoparticles on non-porous surfaces
EP  - 235
SP  - 228
VL  - 812
DO  - 10.1016/j.aca.2014.01.015
ER  - 

@article{
author = "Algarra, Manuel and Radotić, Ksenija and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Savić, Aleksandar G and Jimenez-Jimenez, Jose and Rodriguez-Castellon, Enrique and Esteves da Silva, Joaquim C.G. and Jose Guerrero-Gonzalez, Juan",
year = "2014",
abstract = "A fluorescent nanocomposite based on the inclusion of CdSe quantum dots in porous phosphate heterostructures, functionalized with amino groups (PPH-NH2"CdSe), was synthesized, characterized and used for fingerprint detection. The main scopes of this work were first to develop a friendly chemical powder for detecting latent fingerprints, especially in non-porous surfaces; their further intercalation in PPH structure enables not to spread the fluorescent nanoparticles, for that reason very good fluorescent images can be obtained. The fingerprints, obtained on different non-porous surfaces such as iron tweezers, mobile telephone screen and magnetic band of a credit card, treated with this powder emit a pale orange luminescence under ultraviolet excitation. A further image processing consists of contrast enhancement that allows obtaining positive matches according to the information supplied from a police database, and showed to be more effective than that obtained with the non-processed images. Experimental results illustrate the effectiveness of proposed methods.",
publisher = "Elsevier, Amsterdam",
journal = "Analytica Chimica Acta",
title = "Fingerprint detection and using intercalated CdSe nanoparticles on non-porous surfaces",
pages = "235-228",
volume = "812",
doi = "10.1016/j.aca.2014.01.015"
}

Algarra, M., Radotić, K., Kalauzi, A., Mutavdžić, D., Savić, A. G., Jimenez-Jimenez, J., Rodriguez-Castellon, E., Esteves da Silva, J. C.G.,& Jose Guerrero-Gonzalez, J.. (2014). Fingerprint detection and using intercalated CdSe nanoparticles on non-porous surfaces. in Analytica Chimica Acta
Elsevier, Amsterdam., 812, 228-235.
https://doi.org/10.1016/j.aca.2014.01.015

Algarra M, Radotić K, Kalauzi A, Mutavdžić D, Savić AG, Jimenez-Jimenez J, Rodriguez-Castellon E, Esteves da Silva JC, Jose Guerrero-Gonzalez J. Fingerprint detection and using intercalated CdSe nanoparticles on non-porous surfaces. in Analytica Chimica Acta. 2014;812:228-235.
doi:10.1016/j.aca.2014.01.015 .

Algarra, Manuel, Radotić, Ksenija, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Savić, Aleksandar G, Jimenez-Jimenez, Jose, Rodriguez-Castellon, Enrique, Esteves da Silva, Joaquim C.G., Jose Guerrero-Gonzalez, Juan, "Fingerprint detection and using intercalated CdSe nanoparticles on non-porous surfaces" in Analytica Chimica Acta, 812 (2014):228-235,
https://doi.org/10.1016/j.aca.2014.01.015 . .

TY  - JOUR
AU  - Ducic, Tanja
AU  - Borchert, Manuela
AU  - Savić, Aleksandar G
AU  - Kalauzi, Aleksandar
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/696
AB  - Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Synchrotron Radiation
T1  - Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica
EP  - 346
SP  - 339
VL  - 20
DO  - 10.1107/S0909049512050170
ER  - 

@article{
author = "Ducic, Tanja and Borchert, Manuela and Savić, Aleksandar G and Kalauzi, Aleksandar and Mitrović, Aleksandra Lj. and Radotić, Ksenija",
year = "2013",
abstract = "Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Synchrotron Radiation",
title = "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica",
pages = "346-339",
volume = "20",
doi = "10.1107/S0909049512050170"
}

Ducic, T., Borchert, M., Savić, A. G., Kalauzi, A., Mitrović, A. Lj.,& Radotić, K.. (2013). Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation
Wiley-Blackwell, Hoboken., 20, 339-346.
https://doi.org/10.1107/S0909049512050170

Ducic T, Borchert M, Savić AG, Kalauzi A, Mitrović AL, Radotić K. Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation. 2013;20:339-346.
doi:10.1107/S0909049512050170 .

Ducic, Tanja, Borchert, Manuela, Savić, Aleksandar G, Kalauzi, Aleksandar, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica" in Journal of Synchrotron Radiation, 20 (2013):339-346,
https://doi.org/10.1107/S0909049512050170 . .

TY  - JOUR
AU  - Algarra, Manuel
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Alonso, Beatriz
AU  - Casado, CM
AU  - Esteves da Silva, Joaquim C.G.
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/652
AB  - The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G(5)), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G(5) in the complex, which revealed the presence of a stable complex at a pH of 7. By applying the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5.
PB  - Elsevier Science Bv, Amsterdam
T2  - Talanta
T1  - Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles
EP  - 271
SP  - 267
VL  - 105
DO  - 10.1016/j.talanta.2012.09.008
ER  - 

@article{
author = "Algarra, Manuel and Radotić, Ksenija and Kalauzi, Aleksandar and Alonso, Beatriz and Casado, CM and Esteves da Silva, Joaquim C.G.",
year = "2013",
abstract = "The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G(5)), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G(5) in the complex, which revealed the presence of a stable complex at a pH of 7. By applying the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles",
pages = "271-267",
volume = "105",
doi = "10.1016/j.talanta.2012.09.008"
}

Algarra, M., Radotić, K., Kalauzi, A., Alonso, B., Casado, C.,& Esteves da Silva, J. C.G.. (2013). Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles. in Talanta
Elsevier Science Bv, Amsterdam., 105, 267-271.
https://doi.org/10.1016/j.talanta.2012.09.008

Algarra M, Radotić K, Kalauzi A, Alonso B, Casado C, Esteves da Silva JC. Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles. in Talanta. 2013;105:267-271.
doi:10.1016/j.talanta.2012.09.008 .

Algarra, Manuel, Radotić, Ksenija, Kalauzi, Aleksandar, Alonso, Beatriz, Casado, CM, Esteves da Silva, Joaquim C.G., "Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles" in Talanta, 105 (2013):267-271,
https://doi.org/10.1016/j.talanta.2012.09.008 . .

TY  - JOUR
AU  - Milosavić, Nenad B
AU  - Bogdanović Pristov, Jelena
AU  - Velicković, Dusan V
AU  - Dimitrijević, Aleksandra S
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/574
AB  - BACKGROUND: A totally new approach has been applied for mathematical modeling of the enzyme activity/pH relationship, for quantification and distribution of enzyme activity in and out of carrier pores. This is a very simple and elegant method for determination of the distribution of enzyme molecules on the surface and inside the particles, simply through measurement of enzyme activity at different pH values. RESULTS: Amyloglucosidase (AG) from Aspergillus niger was covalently immobilized onto poly(GMA-co-EGDMA) by the glutaraldehyde and periodate methods. Mathematical modeling of the pH optima for two types of covalently immobilized AG resulted in higher enzyme amounts on the surface within periodate immobilizate (67%) in comparison with glutaraldehyde immobilizate (53%). These values are modified to 64.25% for periodate immobilizate and 49.95% for glutaraldehyde immobilizate when diffusion effects are taken into account. CONCLUSION: The mathematical model applied enabled observation of the difference between the two types of coupling agents and different immobilization procedures throughout quantification of the immobilized enzyme on the matrix surface and inside pores.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima
EP  - 1457
IS  - 10
SP  - 1450
VL  - 87
DO  - 10.1002/jctb.3768
ER  - 

@article{
author = "Milosavić, Nenad B and Bogdanović Pristov, Jelena and Velicković, Dusan V and Dimitrijević, Aleksandra S and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2012",
abstract = "BACKGROUND: A totally new approach has been applied for mathematical modeling of the enzyme activity/pH relationship, for quantification and distribution of enzyme activity in and out of carrier pores. This is a very simple and elegant method for determination of the distribution of enzyme molecules on the surface and inside the particles, simply through measurement of enzyme activity at different pH values. RESULTS: Amyloglucosidase (AG) from Aspergillus niger was covalently immobilized onto poly(GMA-co-EGDMA) by the glutaraldehyde and periodate methods. Mathematical modeling of the pH optima for two types of covalently immobilized AG resulted in higher enzyme amounts on the surface within periodate immobilizate (67%) in comparison with glutaraldehyde immobilizate (53%). These values are modified to 64.25% for periodate immobilizate and 49.95% for glutaraldehyde immobilizate when diffusion effects are taken into account. CONCLUSION: The mathematical model applied enabled observation of the difference between the two types of coupling agents and different immobilization procedures throughout quantification of the immobilized enzyme on the matrix surface and inside pores.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima",
pages = "1457-1450",
number = "10",
volume = "87",
doi = "10.1002/jctb.3768"
}

Milosavić, N. B., Bogdanović Pristov, J., Velicković, D. V., Dimitrijević, A. S., Kalauzi, A.,& Radotić, K.. (2012). Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima. in Journal of Chemical Technology and Biotechnology
Wiley-Blackwell, Hoboken., 87(10), 1450-1457.
https://doi.org/10.1002/jctb.3768

Milosavić NB, Bogdanović Pristov J, Velicković DV, Dimitrijević AS, Kalauzi A, Radotić K. Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima. in Journal of Chemical Technology and Biotechnology. 2012;87(10):1450-1457.
doi:10.1002/jctb.3768 .

Milosavić, Nenad B, Bogdanović Pristov, Jelena, Velicković, Dusan V, Dimitrijević, Aleksandra S, Kalauzi, Aleksandar, Radotić, Ksenija, "Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima" in Journal of Chemical Technology and Biotechnology, 87, no. 10 (2012):1450-1457,
https://doi.org/10.1002/jctb.3768 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Savić, Aleksandar G
AU  - Ristic, Ivan S
AU  - Bajuk-Bogdanović, Danica
AU  - Kalauzi, Aleksandar
AU  - Cakic, Suzana M
AU  - Budinski-Simendic, Jaroslava K
AU  - Jeremic, Milorad G
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/536
AB  - In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers
EP  - 617
IS  - 2
SP  - 607
VL  - 20
DO  - 10.1007/s10924-012-0422-9
ER  - 

@article{
author = "Đikanović, Daniela and Simonović Radosavljević, Jasna and Savić, Aleksandar G and Ristic, Ivan S and Bajuk-Bogdanović, Danica and Kalauzi, Aleksandar and Cakic, Suzana M and Budinski-Simendic, Jaroslava K and Jeremic, Milorad G and Radotić, Ksenija",
year = "2012",
abstract = "In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers",
pages = "617-607",
number = "2",
volume = "20",
doi = "10.1007/s10924-012-0422-9"
}

Đikanović, D., Simonović Radosavljević, J., Savić, A. G., Ristic, I. S., Bajuk-Bogdanović, D., Kalauzi, A., Cakic, S. M., Budinski-Simendic, J. K., Jeremic, M. G.,& Radotić, K.. (2012). Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment
Springer, New York., 20(2), 607-617.
https://doi.org/10.1007/s10924-012-0422-9

Đikanović D, Simonović Radosavljević J, Savić AG, Ristic IS, Bajuk-Bogdanović D, Kalauzi A, Cakic SM, Budinski-Simendic JK, Jeremic MG, Radotić K. Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment. 2012;20(2):607-617.
doi:10.1007/s10924-012-0422-9 .

Đikanović, Daniela, Simonović Radosavljević, Jasna, Savić, Aleksandar G, Ristic, Ivan S, Bajuk-Bogdanović, Danica, Kalauzi, Aleksandar, Cakic, Suzana M, Budinski-Simendic, Jaroslava K, Jeremic, Milorad G, Radotić, Ksenija, "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers" in Journal of Polymers and the Environment, 20, no. 2 (2012):607-617,
https://doi.org/10.1007/s10924-012-0422-9 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Jeremic, Milorad G
AU  - Xu, Jianmin
AU  - Micic, Miodrag
AU  - Whyte, Jeffrey D
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/521
AB  - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of the CdSe quantum dots with plant cell walls
EP  - 47
SP  - 41
VL  - 91
DO  - 10.1016/j.colsurfb.2011.10.032
ER  - 

@article{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Jeremic, Milorad G and Xu, Jianmin and Micic, Miodrag and Whyte, Jeffrey D and Leblanc, Roger M. and Radotić, Ksenija",
year = "2012",
abstract = "There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of the CdSe quantum dots with plant cell walls",
pages = "47-41",
volume = "91",
doi = "10.1016/j.colsurfb.2011.10.032"
}

Đikanović, D., Kalauzi, A., Jeremic, M. G., Xu, J., Micic, M., Whyte, J. D., Leblanc, R. M.,& Radotić, K.. (2012). Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 91, 41-47.
https://doi.org/10.1016/j.colsurfb.2011.10.032

Đikanović D, Kalauzi A, Jeremic MG, Xu J, Micic M, Whyte JD, Leblanc RM, Radotić K. Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces. 2012;91:41-47.
doi:10.1016/j.colsurfb.2011.10.032 .

Đikanović, Daniela, Kalauzi, Aleksandar, Jeremic, Milorad G, Xu, Jianmin, Micic, Miodrag, Whyte, Jeffrey D, Leblanc, Roger M., Radotić, Ksenija, "Interaction of the CdSe quantum dots with plant cell walls" in Colloids and Surfaces B-Biointerfaces, 91 (2012):41-47,
https://doi.org/10.1016/j.colsurfb.2011.10.032 . .