TY  - JOUR
AU  - Stupar, Petar
AU  - Chomicki, Wojciech
AU  - Maillard, Caroline
AU  - Mikeladze, David
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Dietler, Giovanni
AU  - Kasas, Sandor
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1037
AB  - Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.
PB  - Copernicus Gesellschaft Mbh, Gottingen
T2  - Mechanical Sciences
T1  - Mitochondrial activity detected by cantilever based sensor
EP  - 28
IS  - 1
SP  - 23
VL  - 8
DO  - 10.5194/ms-8-23-2017
ER  - 

@article{
author = "Stupar, Petar and Chomicki, Wojciech and Maillard, Caroline and Mikeladze, David and Kalauzi, Aleksandar and Radotić, Ksenija and Dietler, Giovanni and Kasas, Sandor",
year = "2017",
abstract = "Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.",
publisher = "Copernicus Gesellschaft Mbh, Gottingen",
journal = "Mechanical Sciences",
title = "Mitochondrial activity detected by cantilever based sensor",
pages = "28-23",
number = "1",
volume = "8",
doi = "10.5194/ms-8-23-2017"
}

Stupar, P., Chomicki, W., Maillard, C., Mikeladze, D., Kalauzi, A., Radotić, K., Dietler, G.,& Kasas, S.. (2017). Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences
Copernicus Gesellschaft Mbh, Gottingen., 8(1), 23-28.
https://doi.org/10.5194/ms-8-23-2017

Stupar P, Chomicki W, Maillard C, Mikeladze D, Kalauzi A, Radotić K, Dietler G, Kasas S. Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences. 2017;8(1):23-28.
doi:10.5194/ms-8-23-2017 .

Stupar, Petar, Chomicki, Wojciech, Maillard, Caroline, Mikeladze, David, Kalauzi, Aleksandar, Radotić, Ksenija, Dietler, Giovanni, Kasas, Sandor, "Mitochondrial activity detected by cantilever based sensor" in Mechanical Sciences, 8, no. 1 (2017):23-28,
https://doi.org/10.5194/ms-8-23-2017 . .