TY  - JOUR
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Savić, Aleksandar G
AU  - Ristic, Ivan S
AU  - Bajuk-Bogdanović, Danica
AU  - Kalauzi, Aleksandar
AU  - Cakic, Suzana M
AU  - Budinski-Simendic, Jaroslava K
AU  - Jeremic, Milorad G
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/536
AB  - In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers
EP  - 617
IS  - 2
SP  - 607
VL  - 20
DO  - 10.1007/s10924-012-0422-9
ER  - 

@article{
author = "Đikanović, Daniela and Simonović Radosavljević, Jasna and Savić, Aleksandar G and Ristic, Ivan S and Bajuk-Bogdanović, Danica and Kalauzi, Aleksandar and Cakic, Suzana M and Budinski-Simendic, Jaroslava K and Jeremic, Milorad G and Radotić, Ksenija",
year = "2012",
abstract = "In a plant cell wall, lignin is synthesized from several monomeric precursors, combined in various ratios. The variation in monomer type and quantity enables multifunctional role of lignin in plants. Thus, it is important to know how different combinations of lignin monomers impact variability of bond types and local structural changes in the polymer. Lignin model polymers are a good model system for studies of relation between variations of the starting monomers and structural variations within the polymer. We synthesized lignin model polymers from three monomers, CF-based on coniferyl alcohol and ferulic acid in monomer proportions 5:1 and 10:1 (w/w), CP-based on coniferyl alcohol and -coumaric acid in proportion 10:1 (w/w) and CA-based on pure coniferyl alcohol. We studied structural modifications in the obtained polymers, by combining fluorescence microscopy and spectroscopy, FT-IR and Raman spectroscopy, in parallel with determination of polymers' molecular mass distribution. The differences in the low (w) region of the distribution curves of the 10:1 polymers in comparison with the CA polymer may be connected with the increased content of C=C bonds and decreased content of condensed structures, as observed in FT-IR spectra and indicated by the analysis of fluorescence spectra. The 5:1 CF polymer contains a different type of structure in comparison with the 10:1 CF polymers, reflected in its simpler (w) distribution, higher homogeneity of the fluorescence emitting structures and in the appearance of a new high-wavelength emission component. We propose that this component may originate from -conjugated chains, which are longer in this polymer. The results are a contribution to the understanding of the involvement of structural variations of lignin polymers in the cell wall structural plasticity.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers",
pages = "617-607",
number = "2",
volume = "20",
doi = "10.1007/s10924-012-0422-9"
}

Đikanović, D., Simonović Radosavljević, J., Savić, A. G., Ristic, I. S., Bajuk-Bogdanović, D., Kalauzi, A., Cakic, S. M., Budinski-Simendic, J. K., Jeremic, M. G.,& Radotić, K.. (2012). Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment
Springer, New York., 20(2), 607-617.
https://doi.org/10.1007/s10924-012-0422-9

Đikanović D, Simonović Radosavljević J, Savić AG, Ristic IS, Bajuk-Bogdanović D, Kalauzi A, Cakic SM, Budinski-Simendic JK, Jeremic MG, Radotić K. Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers. in Journal of Polymers and the Environment. 2012;20(2):607-617.
doi:10.1007/s10924-012-0422-9 .

Đikanović, Daniela, Simonović Radosavljević, Jasna, Savić, Aleksandar G, Ristic, Ivan S, Bajuk-Bogdanović, Danica, Kalauzi, Aleksandar, Cakic, Suzana M, Budinski-Simendic, Jaroslava K, Jeremic, Milorad G, Radotić, Ksenija, "Structural Differences Between Lignin Model Polymers Synthesized from Various Monomers" in Journal of Polymers and the Environment, 20, no. 2 (2012):607-617,
https://doi.org/10.1007/s10924-012-0422-9 . .

TY  - JOUR
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Jeremic, Milorad G
AU  - Xu, Jianmin
AU  - Micic, Miodrag
AU  - Whyte, Jeffrey D
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/521
AB  - There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Interaction of the CdSe quantum dots with plant cell walls
EP  - 47
SP  - 41
VL  - 91
DO  - 10.1016/j.colsurfb.2011.10.032
ER  - 

@article{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Jeremic, Milorad G and Xu, Jianmin and Micic, Miodrag and Whyte, Jeffrey D and Leblanc, Roger M. and Radotić, Ksenija",
year = "2012",
abstract = "There is an increasing application of quantum dots (QDs) in plant science, as markers for the cells or their cell walls (Cvvs). In a plant cell the CW is a first target place for external agents. We studied interaction of CdSe QDs with CWs isolated from a conifer - Picea omorika (Pant) Purkyne branch. Binding of CdSe QDs was followed by using fluorescence microscopy, fluorescence and FT-IR spectroscopy. The aim of the study was to see whether the QDs induce structural changes in the CW, as well as to find out which kind of interactions between QDs and CVVs occur and to which particular constituent polymers QDs preferably bind. The isolated CW is an appropriate object for study of the interactions with nanoparticles. The results show that in the ON. CdSe predominantly binds to cellulose, via OH groups and to lignin, via the conjugated C=C/C-C chains. The differences in interaction of wet and dry CWs with QDs/chloroform were also studied. In the reaction of the dry CW sample with QDs/chloroform, hydrophobic interactions are dominant. When water was added after QDs/chloroform, hydrophilic interactions enable a partial reconstruction of the C=C chains. The results have an implication on the use of the QDs in plant bioimaging.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Interaction of the CdSe quantum dots with plant cell walls",
pages = "47-41",
volume = "91",
doi = "10.1016/j.colsurfb.2011.10.032"
}

Đikanović, D., Kalauzi, A., Jeremic, M. G., Xu, J., Micic, M., Whyte, J. D., Leblanc, R. M.,& Radotić, K.. (2012). Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 91, 41-47.
https://doi.org/10.1016/j.colsurfb.2011.10.032

Đikanović D, Kalauzi A, Jeremic MG, Xu J, Micic M, Whyte JD, Leblanc RM, Radotić K. Interaction of the CdSe quantum dots with plant cell walls. in Colloids and Surfaces B-Biointerfaces. 2012;91:41-47.
doi:10.1016/j.colsurfb.2011.10.032 .

Đikanović, Daniela, Kalauzi, Aleksandar, Jeremic, Milorad G, Xu, Jianmin, Micic, Miodrag, Whyte, Jeffrey D, Leblanc, Roger M., Radotić, Ksenija, "Interaction of the CdSe quantum dots with plant cell walls" in Colloids and Surfaces B-Biointerfaces, 91 (2012):41-47,
https://doi.org/10.1016/j.colsurfb.2011.10.032 . .

TY  - JOUR
AU  - Mutavdžić, Dragosav
AU  - Xu, Jianmin
AU  - Thakur, Garima
AU  - Triulzi, Robert
AU  - Kasas, Sandor
AU  - Jeremic, Milorad G
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/481
AB  - There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding fluorescence spectrum. It has been found that fluorescence spectroscopy is a simple and reliable methodology to estimate the size of both quantum dot types. For a given solution, the homogeneity of the size of quantum dots is correlated to the relationship between the fluorescence maximum position (FMP) and the quantum dot size. This methodology can be extended to the other fluorescent nanoparticles. The employment of evolving factor analysis and multivariate curve resolution-alternating least squares for decomposition of the series of quantum dots fluorescence spectra recorded by a specific measuring procedure reveals the number of quantum dot fractions having different diameters. The size of the quantum dots in a particular group is defined by the FMP of the corresponding component in the decomposed spectrum. These results show that a combination of the fluorescence and appropriate statistical method for decomposition of the emission spectra of nanoparticles may be a quick and trusted method for the screening of the inhomogeneity of their solution.
PB  - Royal Soc Chemistry, Cambridge
T2  - Analyst
T1  - Determination of the size of quantum dots by fluorescence spectroscopy
EP  - 2396
IS  - 11
SP  - 2391
VL  - 136
DO  - 10.1039/c0an00802h
ER  - 

@article{
author = "Mutavdžić, Dragosav and Xu, Jianmin and Thakur, Garima and Triulzi, Robert and Kasas, Sandor and Jeremic, Milorad G and Leblanc, Roger M. and Radotić, Ksenija",
year = "2011",
abstract = "There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding fluorescence spectrum. It has been found that fluorescence spectroscopy is a simple and reliable methodology to estimate the size of both quantum dot types. For a given solution, the homogeneity of the size of quantum dots is correlated to the relationship between the fluorescence maximum position (FMP) and the quantum dot size. This methodology can be extended to the other fluorescent nanoparticles. The employment of evolving factor analysis and multivariate curve resolution-alternating least squares for decomposition of the series of quantum dots fluorescence spectra recorded by a specific measuring procedure reveals the number of quantum dot fractions having different diameters. The size of the quantum dots in a particular group is defined by the FMP of the corresponding component in the decomposed spectrum. These results show that a combination of the fluorescence and appropriate statistical method for decomposition of the emission spectra of nanoparticles may be a quick and trusted method for the screening of the inhomogeneity of their solution.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Analyst",
title = "Determination of the size of quantum dots by fluorescence spectroscopy",
pages = "2396-2391",
number = "11",
volume = "136",
doi = "10.1039/c0an00802h"
}

Mutavdžić, D., Xu, J., Thakur, G., Triulzi, R., Kasas, S., Jeremic, M. G., Leblanc, R. M.,& Radotić, K.. (2011). Determination of the size of quantum dots by fluorescence spectroscopy. in Analyst
Royal Soc Chemistry, Cambridge., 136(11), 2391-2396.
https://doi.org/10.1039/c0an00802h

Mutavdžić D, Xu J, Thakur G, Triulzi R, Kasas S, Jeremic MG, Leblanc RM, Radotić K. Determination of the size of quantum dots by fluorescence spectroscopy. in Analyst. 2011;136(11):2391-2396.
doi:10.1039/c0an00802h .

Mutavdžić, Dragosav, Xu, Jianmin, Thakur, Garima, Triulzi, Robert, Kasas, Sandor, Jeremic, Milorad G, Leblanc, Roger M., Radotić, Ksenija, "Determination of the size of quantum dots by fluorescence spectroscopy" in Analyst, 136, no. 11 (2011):2391-2396,
https://doi.org/10.1039/c0an00802h . .

TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Đikanović, Daniela
AU  - Jeremic, Milorad G
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/418
AB  - Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Structural Biology
T1  - Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution
EP  - 115
IS  - 1
SP  - 106
VL  - 169
DO  - 10.1016/j.jsb.2009.09.006
ER  - 

@article{
author = "Donaldson, Lloyd and Radotić, Ksenija and Kalauzi, Aleksandar and Đikanović, Daniela and Jeremic, Milorad G",
year = "2010",
abstract = "Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Structural Biology",
title = "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution",
pages = "115-106",
number = "1",
volume = "169",
doi = "10.1016/j.jsb.2009.09.006"
}

Donaldson, L., Radotić, K., Kalauzi, A., Đikanović, D.,& Jeremic, M. G.. (2010). Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology
Academic Press Inc Elsevier Science, San Diego., 169(1), 106-115.
https://doi.org/10.1016/j.jsb.2009.09.006

Donaldson L, Radotić K, Kalauzi A, Đikanović D, Jeremic MG. Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology. 2010;169(1):106-115.
doi:10.1016/j.jsb.2009.09.006 .

Donaldson, Lloyd, Radotić, Ksenija, Kalauzi, Aleksandar, Đikanović, Daniela, Jeremic, Milorad G, "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution" in Journal of Structural Biology, 169, no. 1 (2010):106-115,
https://doi.org/10.1016/j.jsb.2009.09.006 . .