TY  - CONF
AU  - Surudžić, Nevena
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Spasojević, Milica
AU  - Elgahwash, Reyadh Gomah Amar
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2012
AB  - Natural polymers such as alginate, pectin, chitosan etc. were used as carriers for the immobilization of different types of enzymes. Among investigated enzymes, peroxidases hold a special place. Immobilized enzymes are frequently used in phenol removal reactions. In this research horseradish peroxidase was immobilized within alginate micro-beads. This natural polymer was previously oxidized with sodium periodate and modified with tyramine hydrochloride. Percent of oxidation was varied from 2.5 mol% to 10 mol%, and an increase in specific activity was noticed with increasing the oxidation percent. Immobilized peroxidases showed satisfactory stabilities after 10 days of storage. Phenol concentration in a batch reactor decreased during its oxidation with horseradish peroxidase immobilized on tyramine-alginate hydrogels.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
T1  - Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater
EP  - 271
SP  - 267
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2012
ER  - 

@conference{
author = "Surudžić, Nevena and Spasojević, Dragica and Stanković, Mira and Spasojević, Milica and Elgahwash, Reyadh Gomah Amar and Prodanović, Radivoje and Prodanović, Olivera",
year = "2023",
abstract = "Natural polymers such as alginate, pectin, chitosan etc. were used as carriers for the immobilization of different types of enzymes. Among investigated enzymes, peroxidases hold a special place. Immobilized enzymes are frequently used in phenol removal reactions. In this research horseradish peroxidase was immobilized within alginate micro-beads. This natural polymer was previously oxidized with sodium periodate and modified with tyramine hydrochloride. Percent of oxidation was varied from 2.5 mol% to 10 mol%, and an increase in specific activity was noticed with increasing the oxidation percent. Immobilized peroxidases showed satisfactory stabilities after 10 days of storage. Phenol concentration in a batch reactor decreased during its oxidation with horseradish peroxidase immobilized on tyramine-alginate hydrogels.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth and Environmental Research – EcoTER’23",
title = "Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater",
pages = "271-267",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2012"
}

Surudžić, N., Spasojević, D., Stanković, M., Spasojević, M., Elgahwash, R. G. A., Prodanović, R.,& Prodanović, O.. (2023). Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 267-271.
https://hdl.handle.net/21.15107/rcub_rimsi_2012

Surudžić N, Spasojević D, Stanković M, Spasojević M, Elgahwash RGA, Prodanović R, Prodanović O. Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23. 2023;:267-271.
https://hdl.handle.net/21.15107/rcub_rimsi_2012 .

Surudžić, Nevena, Spasojević, Dragica, Stanković, Mira, Spasojević, Milica, Elgahwash, Reyadh Gomah Amar, Prodanović, Radivoje, Prodanović, Olivera, "Horseradish peroxidase immobilization within micro-beads of oxidized tyramine-alginate for phenol removal from wastewater" in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23 (2023):267-271,
https://hdl.handle.net/21.15107/rcub_rimsi_2012 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Surudžić, Nevena
AU  - Đikanović, Daniela
AU  - Simonović Radosavljević, Jasna
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2011
AB  - Phenols are highly toxic organic compounds found in wastewater due to various industries’ pollution. Its removal is of great importance for human and animal health. Enzymatic wastewater treatment has several advantages over traditional methods. Enzyme immobilization onto solid carriers enables its reusability and lowers the cost of treatment. In this work, immobilized horseradish peroxidase on chemically modified alginate hydrogel was tested for phenol removal. The reusability of the tested immobilizate was monitored in repeated cycles. After five consecutive cycles, the remaining activity of the immobilized enzyme was 54%. The obtained result shows the potential for using this immobilizate for wastewater treatment.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
T1  - Wastewater treatment by aminated peroxidase in alginate hydrogel
EP  - 275
SP  - 272
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2011
ER  - 

@conference{
author = "Spasojević, Dragica and Prodanović, Olivera and Surudžić, Nevena and Đikanović, Daniela and Simonović Radosavljević, Jasna and Radotić, Ksenija and Prodanović, Radivoje",
year = "2023",
abstract = "Phenols are highly toxic organic compounds found in wastewater due to various industries’ pollution. Its removal is of great importance for human and animal health. Enzymatic wastewater treatment has several advantages over traditional methods. Enzyme immobilization onto solid carriers enables its reusability and lowers the cost of treatment. In this work, immobilized horseradish peroxidase on chemically modified alginate hydrogel was tested for phenol removal. The reusability of the tested immobilizate was monitored in repeated cycles. After five consecutive cycles, the remaining activity of the immobilized enzyme was 54%. The obtained result shows the potential for using this immobilizate for wastewater treatment.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth and Environmental Research – EcoTER’23",
title = "Wastewater treatment by aminated peroxidase in alginate hydrogel",
pages = "275-272",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2011"
}

Spasojević, D., Prodanović, O., Surudžić, N., Đikanović, D., Simonović Radosavljević, J., Radotić, K.,& Prodanović, R.. (2023). Wastewater treatment by aminated peroxidase in alginate hydrogel. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23
University of Belgrade, Technical Faculty in Bor., 272-275.
https://hdl.handle.net/21.15107/rcub_rimsi_2011

Spasojević D, Prodanović O, Surudžić N, Đikanović D, Simonović Radosavljević J, Radotić K, Prodanović R. Wastewater treatment by aminated peroxidase in alginate hydrogel. in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23. 2023;:272-275.
https://hdl.handle.net/21.15107/rcub_rimsi_2011 .

Spasojević, Dragica, Prodanović, Olivera, Surudžić, Nevena, Đikanović, Daniela, Simonović Radosavljević, Jasna, Radotić, Ksenija, Prodanović, Radivoje, "Wastewater treatment by aminated peroxidase in alginate hydrogel" in 30th International Conference Ecological Truth and Environmental Research – EcoTER’23 (2023):272-275,
https://hdl.handle.net/21.15107/rcub_rimsi_2011 .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica Z.
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2481
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious
disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological
analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may
have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant
staphylococci are found on the skin lesions in animals.
PB  - Springer Nature
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
DO  - 10.1007/s11259-023-10224-9
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica Z. and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2023",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious
disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological
analyses of the affected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the affected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing effect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side effects were noticed. Synthesized lignin oligomers may
have a significant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant
staphylococci are found on the skin lesions in animals.",
publisher = "Springer Nature",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
doi = "10.1007/s11259-023-10224-9"
}

Spasojević, D., Zmejkoski, D. Z., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2023). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications
Springer Nature..
https://doi.org/10.1007/s11259-023-10224-9

Spasojević D, Zmejkoski DZ, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2023;.
doi:10.1007/s11259-023-10224-9 .

Spasojević, Dragica, Zmejkoski, Danica Z., Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications (2023),
https://doi.org/10.1007/s11259-023-10224-9 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Mutavdžić, Dragosav
AU  - Šekuljica, Nataša
AU  - Jovanović, Jelena
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2480
AB  - In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.
PB  - John Wiley and Sons Inc
T2  - Biotechnology Journal
T1  - Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions
SP  - 2300312
DO  - 10.1002/biot.202300312
ER  - 

@article{
author = "Spasojević, Dragica and Prodanović, Olivera and Mutavdžić, Dragosav and Šekuljica, Nataša and Jovanović, Jelena and Maksimović, Vuk and Radotić, Ksenija",
year = "2023",
abstract = "In recent years, versatile peroxidase (VP) has emerged as a promising enzyme for biotechnological applications, as it can oxidize lignin without the external mediators. To gain insights into the breakdown process of artificial lignin by VP, reaction between the two was studied. Degradation products were fractionated using ultrafiltration and analyzed by RP- high performance liquid chromatography with mass detection (HPLC-MS) chromatography. Four fractions were obtained based on their molecular sizes: >10, 3–10, 1–3, and <1 kDa. Interestingly, while VP did not significantly alter the yields of these fractions, the chromatograms revealed the presence of oligomers with different molecular weights (MWs) resulting from the enzymatic activity. The VP exhibits a dual role in its enzymatic activity: both degrading and synthesizing these oligomers. This was confirmed by principal component analysis (PCA). The positive correlations were found between certain oligomers (D1 and D2, D5 and D6, as well as between D7, D10, T2, and T4), suggesting their simultaneous degradation. On the other hand, a negative correlation was found between the monomer and some oligomers (D7, D10, T2, and T4), indicating the decomposition of these oligomers into monomers. These findings shed light on the intricate interplay between VP and artificial lignin, offering valuable insights for potential applications in lignin valorization.",
publisher = "John Wiley and Sons Inc",
journal = "Biotechnology Journal",
title = "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions",
pages = "2300312",
doi = "10.1002/biot.202300312"
}

Spasojević, D., Prodanović, O., Mutavdžić, D., Šekuljica, N., Jovanović, J., Maksimović, V.,& Radotić, K.. (2023). Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal
John Wiley and Sons Inc., 2300312.
https://doi.org/10.1002/biot.202300312

Spasojević D, Prodanović O, Mutavdžić D, Šekuljica N, Jovanović J, Maksimović V, Radotić K. Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions. in Biotechnology Journal. 2023;:2300312.
doi:10.1002/biot.202300312 .

Spasojević, Dragica, Prodanović, Olivera, Mutavdžić, Dragosav, Šekuljica, Nataša, Jovanović, Jelena, Maksimović, Vuk, Radotić, Ksenija, "Two-way reaction of versatile peroxidase with artificial lignin enhances low-molecular weight fractions" in Biotechnology Journal (2023):2300312,
https://doi.org/10.1002/biot.202300312 . .

TY  - CHAP
AU  - Radotić, Ksenija
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica Z.
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2289
AB  - The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.
PB  - The Royal Society of Chemistry
T2  - Lignin-based Materials: Health Care and Medical Applications
T1  - Lignin-based material for biomedical applications: Basic requirements and properties
EP  - 105
SP  - 85
DO  - 10.1039/9781839167843
ER  - 

@inbook{
author = "Radotić, Ksenija and Spasojević, Dragica and Zmejkoski, Danica Z.",
year = "2023",
abstract = "The material selected for biomedical application must be biocompatible, stable against physiological media, non-toxic, non-carcinogenic, corrosion-resistant, bio-inert, and stimuli-responsive, and have a low wear rate. Lignin is the most abundant aromatic polymer on Earth with a complex, recalcitrant structure. A lot of knowledge is acquired on its pre-treatments and processing for biomedical uses in the forms of hydrogels, films, composite materials, nanoparticles, and aerogels. To avoid the potential toxicity and to control the cytotoxicity of lignin-based materials, it is necessary to increase the purity of the starting source materials and understand their reactivity. The poor water solubility, broad molecular weight (MW) distribution, and highly complex structure of lignin restrict its wider clinical applications. These limitations may be effectively improved by chemical modifications or by using lignin fractions with a narrow MW range. Antioxidant and antimicrobial properties allow lignin to be used in pharmaceuticals, drug delivery, and wound healing. It is also reported as a good candidate for composite materials intended for bone regeneration scaffolds in tissue engineering. Future challenges for lignin-based materials for biomedical applications include achieving a better understanding of the structure, isolation, and batch properties of lignin, as well as structure–function relationships in its applications.",
publisher = "The Royal Society of Chemistry",
journal = "Lignin-based Materials: Health Care and Medical Applications",
booktitle = "Lignin-based material for biomedical applications: Basic requirements and properties",
pages = "105-85",
doi = "10.1039/9781839167843"
}

Radotić, K., Spasojević, D.,& Zmejkoski, D. Z.. (2023). Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications
The Royal Society of Chemistry., 85-105.
https://doi.org/10.1039/9781839167843

Radotić K, Spasojević D, Zmejkoski DZ. Lignin-based material for biomedical applications: Basic requirements and properties. in Lignin-based Materials: Health Care and Medical Applications. 2023;:85-105.
doi:10.1039/9781839167843 .

Radotić, Ksenija, Spasojević, Dragica, Zmejkoski, Danica Z., "Lignin-based material for biomedical applications: Basic requirements and properties" in Lignin-based Materials: Health Care and Medical Applications (2023):85-105,
https://doi.org/10.1039/9781839167843 . .

TY  - CONF
AU  - Đikanović, Daniela
AU  - Prodanović, Olivera
AU  - Dragišić Maksimović, Jelena
AU  - Jovanović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Spasojević, Dragica
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1997
AB  - In this study, we investigated, in an in vitro system, the interaction of SiO2 (as a complex with NH4OH) with the peroxidase-catalyzed polymerization of lignin monomer into lignin model compound DHP, imitating conditions of the last step of lignin formation in the cell walls. The structure of obtained polymer was monitored using fluorescent spectroscopy and AFM 30 minutes after the start of synthesis. We studied effect of two different Si concentrations, 0.6 mM and 6 mM. Analysis of the DHP fluorescent spectra, and the application of mathematical decomposition clearly shows that structure of lignin model compound depends on Si concentration in the reaction mixture. The amount of Si affects the distribution of electrons in the DHP polymer. When silicon is present at a concentration of 6 mM, the position of the longest-wavelength APD band shifts towards shorter wavelengths compared to the position of the APD band for 0.6 mM Si. This indicates that during polymer synthesis, higher concentration of silicon may inhibit the formation of pi-electronic structures, which are responsible for electron delocalization. AFM images also show differences in the size and regularity of DHP globules.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 30th International Conference Ecological Truth and Environmental Research - EcoTER'23
T1  - Investigation of silica-lignin interaction. Application of AFM and fluorescence techniques
EP  - 98
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1997
ER  - 

@conference{
author = "Đikanović, Daniela and Prodanović, Olivera and Dragišić Maksimović, Jelena and Jovanović, Jelena and Kalauzi, Aleksandar and Spasojević, Dragica and Radotić, Ksenija",
year = "2023",
abstract = "In this study, we investigated, in an in vitro system, the interaction of SiO2 (as a complex with NH4OH) with the peroxidase-catalyzed polymerization of lignin monomer into lignin model compound DHP, imitating conditions of the last step of lignin formation in the cell walls. The structure of obtained polymer was monitored using fluorescent spectroscopy and AFM 30 minutes after the start of synthesis. We studied effect of two different Si concentrations, 0.6 mM and 6 mM. Analysis of the DHP fluorescent spectra, and the application of mathematical decomposition clearly shows that structure of lignin model compound depends on Si concentration in the reaction mixture. The amount of Si affects the distribution of electrons in the DHP polymer. When silicon is present at a concentration of 6 mM, the position of the longest-wavelength APD band shifts towards shorter wavelengths compared to the position of the APD band for 0.6 mM Si. This indicates that during polymer synthesis, higher concentration of silicon may inhibit the formation of pi-electronic structures, which are responsible for electron delocalization. AFM images also show differences in the size and regularity of DHP globules.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th International Conference Ecological Truth and Environmental Research - EcoTER'23",
title = "Investigation of silica-lignin interaction. Application of AFM and fluorescence techniques",
pages = "98-94",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1997"
}

Đikanović, D., Prodanović, O., Dragišić Maksimović, J., Jovanović, J., Kalauzi, A., Spasojević, D.,& Radotić, K.. (2023). Investigation of silica-lignin interaction. Application of AFM and fluorescence techniques. in 30th International Conference Ecological Truth and Environmental Research - EcoTER'23
University of Belgrade, Technical Faculty in Bor., 94-98.
https://hdl.handle.net/21.15107/rcub_rimsi_1997

Đikanović D, Prodanović O, Dragišić Maksimović J, Jovanović J, Kalauzi A, Spasojević D, Radotić K. Investigation of silica-lignin interaction. Application of AFM and fluorescence techniques. in 30th International Conference Ecological Truth and Environmental Research - EcoTER'23. 2023;:94-98.
https://hdl.handle.net/21.15107/rcub_rimsi_1997 .

Đikanović, Daniela, Prodanović, Olivera, Dragišić Maksimović, Jelena, Jovanović, Jelena, Kalauzi, Aleksandar, Spasojević, Dragica, Radotić, Ksenija, "Investigation of silica-lignin interaction. Application of AFM and fluorescence techniques" in 30th International Conference Ecological Truth and Environmental Research - EcoTER'23 (2023):94-98,
https://hdl.handle.net/21.15107/rcub_rimsi_1997 .

TY  - CONF
AU  - Pantić, Nevena
AU  - Spasojević, Milica
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Balaž, Ana Marija
AU  - Prodanović, Radivoje
AU  - Prodanović, Olivera
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1774
AB  - For the purpose of immobilization, one of the most commonly used enzymes is horseradish peroxidase (HRP). Different carriers can be used as supports for the immobilization of HRP: alginate, pectin, magnetic-beads, macroporous copolymers, silicas etc. Covalent binding of an enzyme to the carrier leads to the formation of strong linkage, thus preventing the enzyme leakage. Macroporous copolymers with different porous characteristics were used for the immobilization of horseradish peroxidase by employing periodate and glutaraldehyde method. Five and 25 mg of HRP were immobilized per gram of the copolymer. Increasing the amount of added enzyme leads to the increase of specific activity of immobilized enzyme. Copolymer with the pore diameter of 297 nm showed the most promising results in terms of specific activity. Immobilized enzymes can be used for the removal of phenolic compounds from waste effluents.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research
T1  - Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal
EP  - 359
SP  - 354
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1774
ER  - 

@conference{
author = "Pantić, Nevena and Spasojević, Milica and Prokopijević, Miloš and Spasojević, Dragica and Balaž, Ana Marija and Prodanović, Radivoje and Prodanović, Olivera",
year = "2022",
abstract = "For the purpose of immobilization, one of the most commonly used enzymes is horseradish peroxidase (HRP). Different carriers can be used as supports for the immobilization of HRP: alginate, pectin, magnetic-beads, macroporous copolymers, silicas etc. Covalent binding of an enzyme to the carrier leads to the formation of strong linkage, thus preventing the enzyme leakage. Macroporous copolymers with different porous characteristics were used for the immobilization of horseradish peroxidase by employing periodate and glutaraldehyde method. Five and 25 mg of HRP were immobilized per gram of the copolymer. Increasing the amount of added enzyme leads to the increase of specific activity of immobilized enzyme. Copolymer with the pore diameter of 297 nm showed the most promising results in terms of specific activity. Immobilized enzymes can be used for the removal of phenolic compounds from waste effluents.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research",
title = "Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal",
pages = "359-354",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1774"
}

Pantić, N., Spasojević, M., Prokopijević, M., Spasojević, D., Balaž, A. M., Prodanović, R.,& Prodanović, O.. (2022). Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal. in 29th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 354-359.
https://hdl.handle.net/21.15107/rcub_rimsi_1774

Pantić N, Spasojević M, Prokopijević M, Spasojević D, Balaž AM, Prodanović R, Prodanović O. Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal. in 29th International Conference Ecological Truth and Environmental Research. 2022;:354-359.
https://hdl.handle.net/21.15107/rcub_rimsi_1774 .

Pantić, Nevena, Spasojević, Milica, Prokopijević, Miloš, Spasojević, Dragica, Balaž, Ana Marija, Prodanović, Radivoje, Prodanović, Olivera, "Covalent immobilization of horseradish peroxidase on novel macroporous poly(GMA-co-EGDMA) for phenol removal" in 29th International Conference Ecological Truth and Environmental Research (2022):354-359,
https://hdl.handle.net/21.15107/rcub_rimsi_1774 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1767
AB  - Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
T1  - PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP
EP  - 363
SP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1767
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Stanković, Mira and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research - EcoTER'22",
title = "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP",
pages = "363-360",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1767"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Stanković, M., Radotić, K.,& Prodanović, R.. (2022). PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
University of Belgrade, Technical Faculty in Bor., 360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Stanković M, Radotić K, Prodanović R. PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22. 2022;:360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Stanković, Mira, Radotić, Ksenija, Prodanović, Radivoje, "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP" in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 (2022):360-363,
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Bartolić, Dragana
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1770
AB  - The application of enzymes for phenol removal from polluted waters is an effective and
environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive
enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic
compounds in the presence of H2O2. The industrial application, however, requires enzyme
immobilization on various carriers to overcome the disadvantages of using the soluble form.
Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional
polymeric network. Immobilization of SBP was performed in an emulsion polymerization
reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of
micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate.
In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios
was tested to determine the carrier with the best performance. Immobilized peroxidase has potential
for application as a biocatalyst for phenol removal from wastewater.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 29th International Conference Ecological Truth and Environmental Research
T1  - Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin
EP  - 353
SP  - 350
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1770
ER  - 

@conference{
author = "Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Olivera and Pantić, Nevena and Bartolić, Dragana and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "The application of enzymes for phenol removal from polluted waters is an effective and
environmentally favorable method and an ongoing worldwide research topic. Oxidoreductive
enzymes, like Soybean peroxidase (SBP), catalyze oxidation and polymerization of phenolic
compounds in the presence of H2O2. The industrial application, however, requires enzyme
immobilization on various carriers to overcome the disadvantages of using the soluble form.
Chemically modified pectin has been chosen as a carrier for entrapment of SBP inside a threedimensional
polymeric network. Immobilization of SBP was performed in an emulsion polymerization
reaction producing enzymes entrapped in covalently crosslinked tyramine-pectin in the shape of
micro-beads. The specific activity of immobilized SBP was determined using pyrogallol as a substrate.
In this study, the stability of the immobilized SBP onto modified pectin in three different molar ratios
was tested to determine the carrier with the best performance. Immobilized peroxidase has potential
for application as a biocatalyst for phenol removal from wastewater.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research",
title = "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin",
pages = "353-350",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1770"
}

Prokopijević, M., Spasojević, D., Prodanović, O., Pantić, N., Bartolić, D., Radotić, K.,& Prodanović, R.. (2022). Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 350-353.
https://hdl.handle.net/21.15107/rcub_rimsi_1770

Prokopijević M, Spasojević D, Prodanović O, Pantić N, Bartolić D, Radotić K, Prodanović R. Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin. in 29th International Conference Ecological Truth and Environmental Research. 2022;:350-353.
https://hdl.handle.net/21.15107/rcub_rimsi_1770 .

Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Olivera, Pantić, Nevena, Bartolić, Dragana, Radotić, Ksenija, Prodanović, Radivoje, "Stability of soybean peroxidase immobilized onto hydrogel micro-beads from tyramine-pectin" in 29th International Conference Ecological Truth and Environmental Research (2022):350-353,
https://hdl.handle.net/21.15107/rcub_rimsi_1770 .

TY  - CONF
AU  - Simonović Radosavljević, Jasna
AU  - Mutavdžić, Dragosav
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1766
AB  - Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood. Tension wood contains more cellulose and less lignin and it is anatomically different from normal wood. Fourier-transform infrared (FTIR) spectroscopy showed that there was no difference in lignin content in segments of bent trees compared to the controls, but the higher amounts of cellulose, xylan, and xyloglucan were observed in bent trees, corresponding to the rearrangements in the cellulose/xyloglucan network.
PB  - Society of Physical Chemists of Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA
EP  - 298
SP  - 295
VL  - I
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1766
ER  - 

@conference{
author = "Simonović Radosavljević, Jasna and Mutavdžić, Dragosav and Spasojević, Dragica and Prokopijević, Miloš and Radotić, Ksenija and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood. Tension wood contains more cellulose and less lignin and it is anatomically different from normal wood. Fourier-transform infrared (FTIR) spectroscopy showed that there was no difference in lignin content in segments of bent trees compared to the controls, but the higher amounts of cellulose, xylan, and xyloglucan were observed in bent trees, corresponding to the rearrangements in the cellulose/xyloglucan network.",
publisher = "Society of Physical Chemists of Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA",
pages = "298-295",
volume = "I",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1766"
}

Simonović Radosavljević, J., Mutavdžić, D., Spasojević, D., Prokopijević, M., Radotić, K.,& Mitrović, A. Lj.. (2022). FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., I, 295-298.
https://hdl.handle.net/21.15107/rcub_rimsi_1766

Simonović Radosavljević J, Mutavdžić D, Spasojević D, Prokopijević M, Radotić K, Mitrović AL. FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;I:295-298.
https://hdl.handle.net/21.15107/rcub_rimsi_1766 .

Simonović Radosavljević, Jasna, Mutavdžić, Dragosav, Spasojević, Dragica, Prokopijević, Miloš, Radotić, Ksenija, Mitrović, Aleksandra Lj., "FTIR ANALYSIS OF NORMAL AND TENSION WOOD IN POPULUS X EURAMERICANA" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, I (2022):295-298,
https://hdl.handle.net/21.15107/rcub_rimsi_1766 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Simonović Radosavljević, Jasna
AU  - Mitrović, Aleksandra Lj.
AU  - Đikanović, Daniela
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1749
AB  - Controlled drug delivery systems are of great interest in modern science and medicine. This study was oriented towards encapsulation of synthetic lignin (DHP) into alginate beads and investigation of its release kinetic. Encapsulation was achieved by the ionotropic gelation method. In vitro release profiles were fitted into four release kinetic models (zero-order, first-order, Higuchi, and Korsmeyer-Peppas). According to the in vitro release, data alginate beads showed initial burst release followed by prolonged release. The best correlation was found for the Korsmeyer-Peppas model (R2 = 0.948), with an n value lower than 0.43, indicating the DHP transport mechanism was controlled by quasi-Fickian diffusion. This delivery system could be promising for chronic wound care and antibacterial treatment.
PB  - Society of Physical Chemists of Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - KINETIC RELEASE STUDY OF SYNTHETIC LIGNIN (DHP) FROM CALCIUM ALGINATE BEADS
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1749
ER  - 

@conference{
author = "Spasojević, Dragica and Mutavdžić, Dragosav and Simonović Radosavljević, Jasna and Mitrović, Aleksandra Lj. and Đikanović, Daniela and Radotić, Ksenija",
year = "2022",
abstract = "Controlled drug delivery systems are of great interest in modern science and medicine. This study was oriented towards encapsulation of synthetic lignin (DHP) into alginate beads and investigation of its release kinetic. Encapsulation was achieved by the ionotropic gelation method. In vitro release profiles were fitted into four release kinetic models (zero-order, first-order, Higuchi, and Korsmeyer-Peppas). According to the in vitro release, data alginate beads showed initial burst release followed by prolonged release. The best correlation was found for the Korsmeyer-Peppas model (R2 = 0.948), with an n value lower than 0.43, indicating the DHP transport mechanism was controlled by quasi-Fickian diffusion. This delivery system could be promising for chronic wound care and antibacterial treatment.",
publisher = "Society of Physical Chemists of Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "KINETIC RELEASE STUDY OF SYNTHETIC LIGNIN (DHP) FROM CALCIUM ALGINATE BEADS",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1749"
}

Spasojević, D., Mutavdžić, D., Simonović Radosavljević, J., Mitrović, A. Lj., Đikanović, D.,& Radotić, K.. (2022). KINETIC RELEASE STUDY OF SYNTHETIC LIGNIN (DHP) FROM CALCIUM ALGINATE BEADS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 2.
https://hdl.handle.net/21.15107/rcub_rimsi_1749

Spasojević D, Mutavdžić D, Simonović Radosavljević J, Mitrović AL, Đikanović D, Radotić K. KINETIC RELEASE STUDY OF SYNTHETIC LIGNIN (DHP) FROM CALCIUM ALGINATE BEADS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;2.
https://hdl.handle.net/21.15107/rcub_rimsi_1749 .

Spasojević, Dragica, Mutavdžić, Dragosav, Simonović Radosavljević, Jasna, Mitrović, Aleksandra Lj., Đikanović, Daniela, Radotić, Ksenija, "KINETIC RELEASE STUDY OF SYNTHETIC LIGNIN (DHP) FROM CALCIUM ALGINATE BEADS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2 (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1749 .

TY  - JOUR
AU  - Prokopijević, Miloš
AU  - Simonović Radosavljević, Jasna
AU  - Spasojević, Dragica
AU  - Vojisavljević, Katarina
AU  - Radotić, Ksenija
AU  - Mitrović, Aleksandra Lj.
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1773
AB  - Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.
PB  - De Gruyter
T2  - Holzforschung
T1  - XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending
EP  - 2505
IS  - 6
SP  - 2500
VL  - 76
DO  - 10.1515/hf-2021-0223
ER  - 

@article{
author = "Prokopijević, Miloš and Simonović Radosavljević, Jasna and Spasojević, Dragica and Vojisavljević, Katarina and Radotić, Ksenija and Mitrović, Aleksandra Lj.",
year = "2022",
abstract = "Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.",
publisher = "De Gruyter",
journal = "Holzforschung",
title = "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending",
pages = "2505-2500",
number = "6",
volume = "76",
doi = "10.1515/hf-2021-0223"
}

Prokopijević, M., Simonović Radosavljević, J., Spasojević, D., Vojisavljević, K., Radotić, K.,& Mitrović, A. Lj.. (2022). XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung
De Gruyter., 76(6), 2500-2505.
https://doi.org/10.1515/hf-2021-0223

Prokopijević M, Simonović Radosavljević J, Spasojević D, Vojisavljević K, Radotić K, Mitrović AL. XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung. 2022;76(6):2500-2505.
doi:10.1515/hf-2021-0223 .

Prokopijević, Miloš, Simonović Radosavljević, Jasna, Spasojević, Dragica, Vojisavljević, Katarina, Radotić, Ksenija, Mitrović, Aleksandra Lj., "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending" in Holzforschung, 76, no. 6 (2022):2500-2505,
https://doi.org/10.1515/hf-2021-0223 . .

TY  - GEN
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2879
AB  - У последњих 20 година, расте интересовање за биополимере, не само међу биохемичарима и молекуларним биолозима, него и у другим научним областима, а посебно у области науке о материјалима. Главне предности биополимера у односу на полимере из фосилних горива, јесу јефтино добијање из обновљивих извора и биоразградивост. Полисахариди су најзаступљенија класа биополимера у природи. Целулоза је главна структурна компонента ћелијског зида виших биљака и по количини најобилнији биополимер на земљи. За њом следи хемицелулоза, која представља скуп хетерополисахарида различите структуре. Најзаступљенији морски полисахарид је алгинат, конституент ћелијског зида мрких морских алги. Полисахариди поседују бројне функционалне групе, погодне за различите врсте хемијских модификација. Јонизабилне групе обезбеђују им хидрофилност, као и способност везивања наелектрисаних молекула и јона. Све ове особине допринеле су да полисахариди постану одличан материјал за производњу хидрогелова. Хидрогелови на бази биополимера нашли су своју примену у индустрији, пољопривреди, фармацији, медицини, заштити животне средине...
PB  - Српско биолошко друштво, Београд
T2  - Treći kongres biologa Srbije
T1  - Biljni polisaharidi kao hidrogelovi
SP  - 281
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2879
ER  - 

@misc{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "У последњих 20 година, расте интересовање за биополимере, не само међу биохемичарима и молекуларним биолозима, него и у другим научним областима, а посебно у области науке о материјалима. Главне предности биополимера у односу на полимере из фосилних горива, јесу јефтино добијање из обновљивих извора и биоразградивост. Полисахариди су најзаступљенија класа биополимера у природи. Целулоза је главна структурна компонента ћелијског зида виших биљака и по количини најобилнији биополимер на земљи. За њом следи хемицелулоза, која представља скуп хетерополисахарида различите структуре. Најзаступљенији морски полисахарид је алгинат, конституент ћелијског зида мрких морских алги. Полисахариди поседују бројне функционалне групе, погодне за различите врсте хемијских модификација. Јонизабилне групе обезбеђују им хидрофилност, као и способност везивања наелектрисаних молекула и јона. Све ове особине допринеле су да полисахариди постану одличан материјал за производњу хидрогелова. Хидрогелови на бази биополимера нашли су своју примену у индустрији, пољопривреди, фармацији, медицини, заштити животне средине...",
publisher = "Српско биолошко друштво, Београд",
journal = "Treći kongres biologa Srbije",
title = "Biljni polisaharidi kao hidrogelovi",
pages = "281",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2879"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Radotić, K.,& Prodanović, R.. (2022). Biljni polisaharidi kao hidrogelovi. in Treći kongres biologa Srbije
Српско биолошко друштво, Београд., 281.
https://hdl.handle.net/21.15107/rcub_rimsi_2879

Spasojević D, Prokopijević M, Prodanović O, Radotić K, Prodanović R. Biljni polisaharidi kao hidrogelovi. in Treći kongres biologa Srbije. 2022;:281.
https://hdl.handle.net/21.15107/rcub_rimsi_2879 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Radotić, Ksenija, Prodanović, Radivoje, "Biljni polisaharidi kao hidrogelovi" in Treći kongres biologa Srbije (2022):281,
https://hdl.handle.net/21.15107/rcub_rimsi_2879 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1759
AB  - INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.
PB  - Prirodno-matematički fakultet Novi Sad
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
T1  - Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads
EP  - 133
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1759
ER  - 

@conference{
author = "Spasojević, Dragica and Stanković, Mira and Prokopijević, Miloš and Prodanović, Olivera and Stojkovska, Jasmina and Obradović, Bojana and Radotić, Ksenija",
year = "2021",
abstract = "INTRODUCTION:
Alginate dressings are widely used in the treatment of exuding wounds29. The enzymatically synthesized lignin model compound dehydrogenate polymer (DHP) from coniferyl alcohol by the enzyme peroxidase, is the best lignin substitute used in
various experiments30. In our previous work, we have shown that synthesized DHP has antibacterial and antibiofilm properties, and in combination with alginate has good potential for wound treatment31.
OBJECTIVES:
The objective of this paper was to study the sustained release of DHP from low and medium viscosity alginate beads.",
publisher = "Prirodno-matematički fakultet Novi Sad",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021",
title = "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads",
pages = "133-132",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1759"
}

Spasojević, D., Stanković, M., Prokopijević, M., Prodanović, O., Stojkovska, J., Obradović, B.,& Radotić, K.. (2021). Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021
Prirodno-matematički fakultet Novi Sad., 132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759

Spasojević D, Stanković M, Prokopijević M, Prodanović O, Stojkovska J, Obradović B, Radotić K. Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021. 2021;:132-133.
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

Spasojević, Dragica, Stanković, Mira, Prokopijević, Miloš, Prodanović, Olivera, Stojkovska, Jasmina, Obradović, Bojana, Radotić, Ksenija, "Sustained release of lignin model compound dehydrogenate polymer (DHP) from alginate beads" in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference IBSC2021 (2021):132-133,
https://hdl.handle.net/21.15107/rcub_rimsi_1759 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pantić, Nevena
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1790
AB  - Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.
PB  - Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization
EP  - 342
SP  - 340
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1790
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Radotić, Ksenija and Prodanović, Radivoje",
year = "2021",
abstract = "Hemicellulose rich in xylan was isolated from corncobs. Functional groups were introduced first by
carboxymethylation and then by coupling with tyramine via carbodiimide-mediated reaction.
Obtained hydrogel was tested for horseradish peroxidase (HRP) immobilization within microbeads,
formed in an emulsion based enzymatic polymerization reaction. This model system showed that
modified hemicellulose isolated from corncob is a suitable candidate for enzyme or small molecule
immobilization for different purposes.",
publisher = "Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization",
pages = "342-340",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1790"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Radotić, K.,& Prodanović, R.. (2021). Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 1, 340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790

Spasojević D, Prokopijević M, Prodanović O, Pantić N, Radotić K, Prodanović R. Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;1:340-342.
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Radotić, Ksenija, Prodanović, Radivoje, "Chemical modification of hemicellulose isolated from corncobs to obtain hydrogel for enzyme immobilization" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2021):340-342,
https://hdl.handle.net/21.15107/rcub_rimsi_1790 .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Simonović Radosavljević, Jasna
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Kovacević, Jovana
AU  - Prodanović, Olivera
AU  - Todorović, Bratislav
AU  - Matović, Branko
AU  - Stanković, Mira
AU  - Maksimović, Vuk
AU  - Mutavdžić, Dragosav
AU  - Skocic, Milos
AU  - Pesic, Mirjana
AU  - Prokic, Ljiljana
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1449
AB  - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Cell wall response to UV radiation in needles of Picea omorika
EP  - 190
SP  - 176
VL  - 161
DO  - 10.1016/j.plaphy.2021.02.007
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovacević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skocic, Milos and Pesic, Mirjana and Prokic, Ljiljana and Radotić, Ksenija",
year = "2021",
abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Cell wall response to UV radiation in needles of Picea omorika",
pages = "190-176",
volume = "161",
doi = "10.1016/j.plaphy.2021.02.007"
}

Mitrović, A. Lj., Simonović Radosavljević, J., Prokopijević, M., Spasojević, D., Kovacević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skocic, M., Pesic, M., Prokic, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 161, 176-190.
https://doi.org/10.1016/j.plaphy.2021.02.007

Mitrović AL, Simonović Radosavljević J, Prokopijević M, Spasojević D, Kovacević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skocic M, Pesic M, Prokic L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190.
doi:10.1016/j.plaphy.2021.02.007 .

Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovacević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skocic, Milos, Pesic, Mirjana, Prokic, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190,
https://doi.org/10.1016/j.plaphy.2021.02.007 . .

TY  - CONF
AU  - Pantić, Nevena
AU  - Popović, Nikolina
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Radivoje
AU  - Đikanović, Daniela
AU  - Prodanović, Olivera
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1656
AB  - Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL
SP  - 220-223
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1656
ER  - 

@conference{
author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Đikanović, Daniela and Prodanović, Olivera",
year = "2019",
abstract = "Phenolic compounds are one of the most common pollutants in aqueous systems, so their removal
from water is of major interest. Among biocatalysts used for phenol removal, horseradish peroxidase
is the most investigated for this purpose. Enzyme inactivation is a major problem which could be
successfully overcome by immobilization of the enzyme onto different polymers. Tyramine-alginate
micro-beads were tested for the immobilization of horseradish peroxidase. Different concentrations of
tyramine-alginate were used and their influence on specific activity of the enzyme was tested.
Increasing concentration of oxidized alginate results in increase of specific activity. Immobilized HRP
was tested for phenol removal in a batch reactor. Presented results were obtained with HRP
immobilized within 10 mol% tyramine-alginate micro-beads. These biocatalysts can be used up to
three cycles.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL",
pages = "220-223",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1656"
}

Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Đikanović, D.,& Prodanović, O.. (2019). OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 220-223.
https://hdl.handle.net/21.15107/rcub_rimsi_1656

Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Đikanović D, Prodanović O. OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL. in 27th International Conference Ecological Truth and Environmental Research. 2019;:220-223.
https://hdl.handle.net/21.15107/rcub_rimsi_1656 .

Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Đikanović, Daniela, Prodanović, Olivera, "OPTIMIZATION OF HORSERADISH PEROXIDASE ENCAPSULATION WITHIN TYRAMINE-ALGINATE FOR PHENOL REMOVAL" in 27th International Conference Ecological Truth and Environmental Research (2019):220-223,
https://hdl.handle.net/21.15107/rcub_rimsi_1656 .

TY  - CONF
AU  - Stanković, Mira
AU  - Simonović Radosavljević, Jasna
AU  - Đikanović, Daniela
AU  - Spasojević, Dragica
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1807
AB  - In this pilot research, we showed that pollen autofluorescence varied between the pollen of the
different botanical species. Our findings suggest that classification of pollen grains may be obtained
based on their fluorescence images.
PB  - University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia
PB  - Serbian Society for Microscopy, Serbia
C3  - 14th MULTINATIONAL CONGRESS ON MICROSCOPY
T1  - The use of fluorescence microscopy for classification of pollen grains
SP  - 171-173
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1807
ER  - 

@conference{
author = "Stanković, Mira and Simonović Radosavljević, Jasna and Đikanović, Daniela and Spasojević, Dragica and Radotić, Ksenija",
year = "2019",
abstract = "In this pilot research, we showed that pollen autofluorescence varied between the pollen of the
different botanical species. Our findings suggest that classification of pollen grains may be obtained
based on their fluorescence images.",
publisher = "University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Serbian Society for Microscopy, Serbia",
journal = "14th MULTINATIONAL CONGRESS ON MICROSCOPY",
title = "The use of fluorescence microscopy for classification of pollen grains",
pages = "171-173",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1807"
}

Stanković, M., Simonović Radosavljević, J., Đikanović, D., Spasojević, D.,& Radotić, K.. (2019). The use of fluorescence microscopy for classification of pollen grains. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY
University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia., 171-173.
https://hdl.handle.net/21.15107/rcub_rimsi_1807

Stanković M, Simonović Radosavljević J, Đikanović D, Spasojević D, Radotić K. The use of fluorescence microscopy for classification of pollen grains. in 14th MULTINATIONAL CONGRESS ON MICROSCOPY. 2019;:171-173.
https://hdl.handle.net/21.15107/rcub_rimsi_1807 .

Stanković, Mira, Simonović Radosavljević, Jasna, Đikanović, Daniela, Spasojević, Dragica, Radotić, Ksenija, "The use of fluorescence microscopy for classification of pollen grains" in 14th MULTINATIONAL CONGRESS ON MICROSCOPY (2019):171-173,
https://hdl.handle.net/21.15107/rcub_rimsi_1807 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Pantić, Nevena
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Simonović Radosavljević, Jasna
AU  - Đikanović, Daniela
AU  - Prodanović, Radivoje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1657
AB  - Horseradish peroxidase (HRP, E.C. 1.11.1.7) catalyzes oxidation of aqueous aromatic compounds
using hydrogen peroxide. Enzymatic treatment methods for phenol removal from wastewaters has
become an efficient and environmentally friendly alternative for the traditional methods.
Carboxymethyl cellulose (CMC) derivative with tyramine attached via amide bond to carboxyl groups
has been chosen as carrier for immobilization. In effort to overcome the main disadvantage of
entrapment immobilization method, loss of enzyme activity due to washing out from the carrier, HRP
was modified in a reductive amination reaction and tyramine was bound to the enzyme.
Immobilization of tyramine-HRP onto tyramide-carboxymethyl cellulose carrier was carried in an
emulsion polymerization reaction that produced carboxymethyl cellulose microbeads. The highest
specific activity of the obtained biocatalyst was 0.227 U/ml and after 48h of storage 0.197 U/ml.
Immobilized tyramine-HRP retained 87% of activity after 48 h. Immobilized HRP is a suitable
candidate for wastewater treatment.
PB  - University of Belgrade, Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research
T1  - IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT
SP  - 224-227
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1657
ER  - 

@conference{
author = "Prokopijević, Miloš and Pantić, Nevena and Spasojević, Dragica and Prodanović, Olivera and Simonović Radosavljević, Jasna and Đikanović, Daniela and Prodanović, Radivoje",
year = "2019",
abstract = "Horseradish peroxidase (HRP, E.C. 1.11.1.7) catalyzes oxidation of aqueous aromatic compounds
using hydrogen peroxide. Enzymatic treatment methods for phenol removal from wastewaters has
become an efficient and environmentally friendly alternative for the traditional methods.
Carboxymethyl cellulose (CMC) derivative with tyramine attached via amide bond to carboxyl groups
has been chosen as carrier for immobilization. In effort to overcome the main disadvantage of
entrapment immobilization method, loss of enzyme activity due to washing out from the carrier, HRP
was modified in a reductive amination reaction and tyramine was bound to the enzyme.
Immobilization of tyramine-HRP onto tyramide-carboxymethyl cellulose carrier was carried in an
emulsion polymerization reaction that produced carboxymethyl cellulose microbeads. The highest
specific activity of the obtained biocatalyst was 0.227 U/ml and after 48h of storage 0.197 U/ml.
Immobilized tyramine-HRP retained 87% of activity after 48 h. Immobilized HRP is a suitable
candidate for wastewater treatment.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research",
title = "IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT",
pages = "224-227",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1657"
}

Prokopijević, M., Pantić, N., Spasojević, D., Prodanović, O., Simonović Radosavljević, J., Đikanović, D.,& Prodanović, R.. (2019). IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT. in 27th International Conference Ecological Truth and Environmental Research
University of Belgrade, Technical Faculty in Bor., 224-227.
https://hdl.handle.net/21.15107/rcub_rimsi_1657

Prokopijević M, Pantić N, Spasojević D, Prodanović O, Simonović Radosavljević J, Đikanović D, Prodanović R. IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT. in 27th International Conference Ecological Truth and Environmental Research. 2019;:224-227.
https://hdl.handle.net/21.15107/rcub_rimsi_1657 .

Prokopijević, Miloš, Pantić, Nevena, Spasojević, Dragica, Prodanović, Olivera, Simonović Radosavljević, Jasna, Đikanović, Daniela, Prodanović, Radivoje, "IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT" in 27th International Conference Ecological Truth and Environmental Research (2019):224-227,
https://hdl.handle.net/21.15107/rcub_rimsi_1657 .

TY  - JOUR
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Sikoparija, B.
AU  - Spasojević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Natic, Maja
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1238
AB  - It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution-alternating least squares (MCR-ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr)(fl) , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr)(sp) was calculated. The values of the (Ph/Pr)(fl) ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr)(fl) and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr)(sp), in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.
PB  - Springer, New York
T2  - Journal of Applied Spectroscopy
T1  - Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR-ALS Analysis
EP  - 263
IS  - 2
SP  - 256
VL  - 86
DO  - 10.1007/s10812-019-00809-1
ER  - 

@article{
author = "Stanković, Mira and Bartolić, Dragana and Sikoparija, B. and Spasojević, Dragica and Mutavdžić, Dragosav and Natic, Maja and Radotić, Ksenija",
year = "2019",
abstract = "It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution-alternating least squares (MCR-ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr)(fl) , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr)(sp) was calculated. The values of the (Ph/Pr)(fl) ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr)(fl) and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr)(sp), in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.",
publisher = "Springer, New York",
journal = "Journal of Applied Spectroscopy",
title = "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR-ALS Analysis",
pages = "263-256",
number = "2",
volume = "86",
doi = "10.1007/s10812-019-00809-1"
}

Stanković, M., Bartolić, D., Sikoparija, B., Spasojević, D., Mutavdžić, D., Natic, M.,& Radotić, K.. (2019). Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR-ALS Analysis. in Journal of Applied Spectroscopy
Springer, New York., 86(2), 256-263.
https://doi.org/10.1007/s10812-019-00809-1

Stanković M, Bartolić D, Sikoparija B, Spasojević D, Mutavdžić D, Natic M, Radotić K. Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR-ALS Analysis. in Journal of Applied Spectroscopy. 2019;86(2):256-263.
doi:10.1007/s10812-019-00809-1 .

Stanković, Mira, Bartolić, Dragana, Sikoparija, B., Spasojević, Dragica, Mutavdžić, Dragosav, Natic, Maja, Radotić, Ksenija, "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR-ALS Analysis" in Journal of Applied Spectroscopy, 86, no. 2 (2019):256-263,
https://doi.org/10.1007/s10812-019-00809-1 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Zelenović, Nevena
AU  - Polović, Natalija
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1196
AB  - Derivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52 +/- 25 mu m and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 degrees C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity.
PB  - Polymer Soc Korea, Seoul
T2  - Macromolecular Research
T1  - Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation
EP  - 771
IS  - 8
SP  - 764
VL  - 27
DO  - 10.1007/s13233-019-7111-7
ER  - 

@article{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Zelenović, Nevena and Polović, Natalija and Radotić, Ksenija and Prodanović, Radivoje",
year = "2019",
abstract = "Derivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52 +/- 25 mu m and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 degrees C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity.",
publisher = "Polymer Soc Korea, Seoul",
journal = "Macromolecular Research",
title = "Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation",
pages = "771-764",
number = "8",
volume = "27",
doi = "10.1007/s13233-019-7111-7"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Zelenović, N., Polović, N., Radotić, K.,& Prodanović, R.. (2019). Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation. in Macromolecular Research
Polymer Soc Korea, Seoul., 27(8), 764-771.
https://doi.org/10.1007/s13233-019-7111-7

Spasojević D, Prokopijević M, Prodanović O, Zelenović N, Polović N, Radotić K, Prodanović R. Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation. in Macromolecular Research. 2019;27(8):764-771.
doi:10.1007/s13233-019-7111-7 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Zelenović, Nevena, Polović, Natalija, Radotić, Ksenija, Prodanović, Radivoje, "Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation" in Macromolecular Research, 27, no. 8 (2019):764-771,
https://doi.org/10.1007/s13233-019-7111-7 . .

TY  - CONF
AU  - Kovačević, Ana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Miljković, Radmila
AU  - Inic-Kanada, Aleksandra
AU  - Spasojević, Dragica
AU  - Radotić, Ksenija
AU  - Stojanović, Marijana M.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2870
AB  - The dehydrogenate polymer from coniferyl alcohol (DHP; a lignin model compound) in alginate hydrogel (ALG) has been shown to exert a strong antibacterial activity. To broadens a spectrum of potential DHP/ALG application, we aimed this study to evaluate the immunomodulatory activity of DHP/ALG. DHP and ALG were tested separately and in mixture (1:2 w/w) for their impact on in vitro production of cytokines (IL-6, IL-12, and IL-10) and reactive oxygen (ROS) and nitrogen (RNS) species by resident (RMs) and thioglycolate-elicited (TGMs) peritoneal macrophages of BALB/c mice. RMs and TGMs were stimulated (48h) with ALG and DHP in concentrations previously shown to be non-cytotoxic (up to 50 and 25 μg/ml, respectively). DHP/ALG promotes simultaneous production of inflammatory (IL-6, IL-12) and regulatory cytokines by RMs in a positive dose-dependent manner. Production of inflammatory cytokines was stimulated by ALG, while an increase in IL-10 production positively correlated to the concentration of DHP. ALG also stimulated the production of IL-12 by TGMs, which was mirrored in the outcome of ALG/DHP stimulation. The significant increase in the activity of myeloperoxidase (MPO) due to DHP and/or ALG stimulation was recorded in TGMs, while a slight increase in MPO activity in RMs was recorded only upon stimulation with the higher amount of ALG. ALG in a positive dose-dependent manner stimulated the production of ROS and RNS by both RMS and TGMs. In all cases, except ROS production by RMs, the impact of ALG stimulation was mirrored in the outcome of ALG/DHP stimulation. Our results suggest that DHP/ALG exerts an immunomodulatory activity that could complement already reported antimicrobial activity and warrants further investigation on the use of DHP/ALG in the treatment of infectious diseases.
PB  - Institute for Biological Research "Siniša Stanković"
C3  - Immunology at the confluence of multidisciplinary approaches
T1  - Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2870
ER  - 

@conference{
author = "Kovačević, Ana and Lukić, Ivana and Marinković, Emilija and Miljković, Radmila and Inic-Kanada, Aleksandra and Spasojević, Dragica and Radotić, Ksenija and Stojanović, Marijana M.",
year = "2019",
abstract = "The dehydrogenate polymer from coniferyl alcohol (DHP; a lignin model compound) in alginate hydrogel (ALG) has been shown to exert a strong antibacterial activity. To broadens a spectrum of potential DHP/ALG application, we aimed this study to evaluate the immunomodulatory activity of DHP/ALG. DHP and ALG were tested separately and in mixture (1:2 w/w) for their impact on in vitro production of cytokines (IL-6, IL-12, and IL-10) and reactive oxygen (ROS) and nitrogen (RNS) species by resident (RMs) and thioglycolate-elicited (TGMs) peritoneal macrophages of BALB/c mice. RMs and TGMs were stimulated (48h) with ALG and DHP in concentrations previously shown to be non-cytotoxic (up to 50 and 25 μg/ml, respectively). DHP/ALG promotes simultaneous production of inflammatory (IL-6, IL-12) and regulatory cytokines by RMs in a positive dose-dependent manner. Production of inflammatory cytokines was stimulated by ALG, while an increase in IL-10 production positively correlated to the concentration of DHP. ALG also stimulated the production of IL-12 by TGMs, which was mirrored in the outcome of ALG/DHP stimulation. The significant increase in the activity of myeloperoxidase (MPO) due to DHP and/or ALG stimulation was recorded in TGMs, while a slight increase in MPO activity in RMs was recorded only upon stimulation with the higher amount of ALG. ALG in a positive dose-dependent manner stimulated the production of ROS and RNS by both RMS and TGMs. In all cases, except ROS production by RMs, the impact of ALG stimulation was mirrored in the outcome of ALG/DHP stimulation. Our results suggest that DHP/ALG exerts an immunomodulatory activity that could complement already reported antimicrobial activity and warrants further investigation on the use of DHP/ALG in the treatment of infectious diseases.",
publisher = "Institute for Biological Research "Siniša Stanković"",
journal = "Immunology at the confluence of multidisciplinary approaches",
title = "Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2870"
}

Kovačević, A., Lukić, I., Marinković, E., Miljković, R., Inic-Kanada, A., Spasojević, D., Radotić, K.,& Stojanović, M. M.. (2019). Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate. in Immunology at the confluence of multidisciplinary approaches
Institute for Biological Research "Siniša Stanković"., 129.
https://hdl.handle.net/21.15107/rcub_rimsi_2870

Kovačević A, Lukić I, Marinković E, Miljković R, Inic-Kanada A, Spasojević D, Radotić K, Stojanović MM. Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate. in Immunology at the confluence of multidisciplinary approaches. 2019;:129.
https://hdl.handle.net/21.15107/rcub_rimsi_2870 .

Kovačević, Ana, Lukić, Ivana, Marinković, Emilija, Miljković, Radmila, Inic-Kanada, Aleksandra, Spasojević, Dragica, Radotić, Ksenija, Stojanović, Marijana M., "Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate" in Immunology at the confluence of multidisciplinary approaches (2019):129,
https://hdl.handle.net/21.15107/rcub_rimsi_2870 .

TY  - CONF
AU  - Pantić, Nevena
AU  - Popović, Nikolina
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Radivoje
AU  - Radotić, Ksenija
AU  - Prodanović, Olivera
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1764
AB  - Removal of phenolic compounds from wastewaters was previously studied using different enzymatic approaches. In the presence of hydrogen peroxide, peroxidases are able to oxidize phenol-like compounds and form non-soluble polymers that could be easily removed from aqueous
phase. Horseradish peroxidase (HRP) is the most investigated peroxidase used for phenol removal from waste effluents, but it can be easily inactivated during this process by excess of hydrogen peroxide. In order to increase operational stability of the enzyme, immobilization on different materials and various peroxide delivery systems were tested. In our previous work, we studied bioinspired hydrogels based on natural cell wall polymers and enzymes, for efficient removal of phenols from water. In this work, tyramine-alginate hydrogels that we have previously developed were used for horseradish peroxidase encapsulation within micro-beads obtained in a coupled emulsion polymerization reaction. The aim of this research was to study the influence of tyramine-alginate concentration and hydrogen peroxide delivery system on operational stability and efficiency of phenol removal by immobilized peroxidase. The best result of 96% phenol removal from water solution was achieved by peroxidase immobilized within 20% (w/v) tyramine-alginate micro-beads using delivery system for hydrogen peroxide composed of 0.187 U mL-1 of glucose oxidase and 4 mmol L-1 of glucose. The reusability studies showed that these biocatalysts can be used up to five cycles with slight decrease in their catalytic performance.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković”, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1764
ER  - 

@conference{
author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Radotić, Ksenija and Prodanović, Olivera",
year = "2018",
abstract = "Removal of phenolic compounds from wastewaters was previously studied using different enzymatic approaches. In the presence of hydrogen peroxide, peroxidases are able to oxidize phenol-like compounds and form non-soluble polymers that could be easily removed from aqueous
phase. Horseradish peroxidase (HRP) is the most investigated peroxidase used for phenol removal from waste effluents, but it can be easily inactivated during this process by excess of hydrogen peroxide. In order to increase operational stability of the enzyme, immobilization on different materials and various peroxide delivery systems were tested. In our previous work, we studied bioinspired hydrogels based on natural cell wall polymers and enzymes, for efficient removal of phenols from water. In this work, tyramine-alginate hydrogels that we have previously developed were used for horseradish peroxidase encapsulation within micro-beads obtained in a coupled emulsion polymerization reaction. The aim of this research was to study the influence of tyramine-alginate concentration and hydrogen peroxide delivery system on operational stability and efficiency of phenol removal by immobilized peroxidase. The best result of 96% phenol removal from water solution was achieved by peroxidase immobilized within 20% (w/v) tyramine-alginate micro-beads using delivery system for hydrogen peroxide composed of 0.187 U mL-1 of glucose oxidase and 4 mmol L-1 of glucose. The reusability studies showed that these biocatalysts can be used up to five cycles with slight decrease in their catalytic performance.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads",
pages = "157",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1764"
}

Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Radotić, K.,& Prodanović, O.. (2018). Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 157.
https://hdl.handle.net/21.15107/rcub_rimsi_1764

Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Radotić K, Prodanović O. Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:157.
https://hdl.handle.net/21.15107/rcub_rimsi_1764 .

Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Radotić, Ksenija, Prodanović, Olivera, "Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):157,
https://hdl.handle.net/21.15107/rcub_rimsi_1764 .

TY  - CONF
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Olivera
AU  - Stanković, Mira
AU  - Pantić, Nevena
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1769
AB  - Pectin is a natural biopolymer contained in the plant cell wall. Ability of pectin to form hydrogels
has been widely investigated for various purposes from food industry to biomedical applications.
The goal of our study was to create modified pectin with improved gelling properties, that could
produce stable covalently cross-linked hydrogels. Aromatic groups were introduced into the previously oxidized pectin chains in a reductive amination reaction with tyramine and cross-linking
was achieved through enzyme reaction with soybean peroxidase in the presence of hydrogen
peroxide. Characterization confirmed that a series of obtained tyramine-pectins (oxidized from 2.5
to 20 mol%) had different degrees of modification. UV spectra confirmed the presence of tyramine
group with the absorbance peak at 275 nm. Chemical shifts of modified pectin in 1
H NMR spectra
correspond to newly formed functional groups. The presence of C-C in-ring stretching vibrations
peaks at 1518 and 1417 cm-1 in FTIR spectra of modified pectin confirmed the presence of aromatic rings. Increased nitrogen percentage in elemental analysis and additional ionizable group on
the titration curve of tyramine-pectin proved the introduction of positively charged amino group.
Surface morphologies also showed certain differences under SEM-EDS. Tyramine-pectin has been
successfully tested as a carrier for enzyme immobilization, but potential applications of this material could be in tissue engineering, drug delivery or wound healing.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković”, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Characterization of chemically modified pectins as novel material for various applications
SP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1769
ER  - 

@conference{
author = "Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Olivera and Stanković, Mira and Pantić, Nevena and Radotić, Ksenija and Prodanović, Radivoje",
year = "2018",
abstract = "Pectin is a natural biopolymer contained in the plant cell wall. Ability of pectin to form hydrogels
has been widely investigated for various purposes from food industry to biomedical applications.
The goal of our study was to create modified pectin with improved gelling properties, that could
produce stable covalently cross-linked hydrogels. Aromatic groups were introduced into the previously oxidized pectin chains in a reductive amination reaction with tyramine and cross-linking
was achieved through enzyme reaction with soybean peroxidase in the presence of hydrogen
peroxide. Characterization confirmed that a series of obtained tyramine-pectins (oxidized from 2.5
to 20 mol%) had different degrees of modification. UV spectra confirmed the presence of tyramine
group with the absorbance peak at 275 nm. Chemical shifts of modified pectin in 1
H NMR spectra
correspond to newly formed functional groups. The presence of C-C in-ring stretching vibrations
peaks at 1518 and 1417 cm-1 in FTIR spectra of modified pectin confirmed the presence of aromatic rings. Increased nitrogen percentage in elemental analysis and additional ionizable group on
the titration curve of tyramine-pectin proved the introduction of positively charged amino group.
Surface morphologies also showed certain differences under SEM-EDS. Tyramine-pectin has been
successfully tested as a carrier for enzyme immobilization, but potential applications of this material could be in tissue engineering, drug delivery or wound healing.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Characterization of chemically modified pectins as novel material for various applications",
pages = "120",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1769"
}

Prokopijević, M., Spasojević, D., Prodanović, O., Stanković, M., Pantić, N., Radotić, K.,& Prodanović, R.. (2018). Characterization of chemically modified pectins as novel material for various applications. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 120.
https://hdl.handle.net/21.15107/rcub_rimsi_1769

Prokopijević M, Spasojević D, Prodanović O, Stanković M, Pantić N, Radotić K, Prodanović R. Characterization of chemically modified pectins as novel material for various applications. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:120.
https://hdl.handle.net/21.15107/rcub_rimsi_1769 .

Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Olivera, Stanković, Mira, Pantić, Nevena, Radotić, Ksenija, Prodanović, Radivoje, "Characterization of chemically modified pectins as novel material for various applications" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):120,
https://hdl.handle.net/21.15107/rcub_rimsi_1769 .

TY  - CONF
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1789
AB  - Phenols are hazardous substances that enter into the environment as byproducts of various industries. The immobilizate of aminated horseradish
peroxidase (HRP) onto oxidized alginate beads was tested for reusability in
the removal of aqueous phenol. In the present study, the possibility of
continuous phenol removal using immobilized aminated HRP on alginate
beads was shown to be promising. After five repeated cycles, the efficiency
of HRP immobilizate for phenol removal has decreased by 46%
PB  - Society of Physical Chemists of Serbia
C3  - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Reusability of alginate beads with immobilized aminated HRP for phenol removal from water
EP  - 804
SP  - 801
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1789
ER  - 

@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Radotić, Ksenija and Prodanović, Radivoje",
year = "2018",
abstract = "Phenols are hazardous substances that enter into the environment as byproducts of various industries. The immobilizate of aminated horseradish
peroxidase (HRP) onto oxidized alginate beads was tested for reusability in
the removal of aqueous phenol. In the present study, the possibility of
continuous phenol removal using immobilized aminated HRP on alginate
beads was shown to be promising. After five repeated cycles, the efficiency
of HRP immobilizate for phenol removal has decreased by 46%",
publisher = "Society of Physical Chemists of Serbia",
journal = "14th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Reusability of alginate beads with immobilized aminated HRP for phenol removal from water",
pages = "804-801",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1789"
}

Spasojević, D., Prokopijević, M., Prodanović, O., Radotić, K.,& Prodanović, R.. (2018). Reusability of alginate beads with immobilized aminated HRP for phenol removal from water. in 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 2, 801-804.
https://hdl.handle.net/21.15107/rcub_rimsi_1789

Spasojević D, Prokopijević M, Prodanović O, Radotić K, Prodanović R. Reusability of alginate beads with immobilized aminated HRP for phenol removal from water. in 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2018;2:801-804.
https://hdl.handle.net/21.15107/rcub_rimsi_1789 .

Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Radotić, Ksenija, Prodanović, Radivoje, "Reusability of alginate beads with immobilized aminated HRP for phenol removal from water" in 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2 (2018):801-804,
https://hdl.handle.net/21.15107/rcub_rimsi_1789 .