TY  - GEN
AU  - Bartolić, Dragana
AU  - Lucev, Milica
AU  - Stanković, Mira
AU  - Prokopijević, Miloš
AU  - Algara, Manuel
AU  - Stanković, Slavica
AU  - Radotić, Ksenija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2374
AB  - Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.
PB  - University of Belgrade, Technical Faculty in Bor
T2  - EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
T1  - ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus
EP  - 54
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2374
ER  - 

@misc{
author = "Bartolić, Dragana and Lucev, Milica and Stanković, Mira and Prokopijević, Miloš and Algara, Manuel and Stanković, Slavica and Radotić, Ksenija",
year = "2023",
abstract = "Environmental occurrence of the pathogenic fungus Aspergillus flavus (A. flavus) has hazardous
effects on the health status of plants, humans, and animals. It is important to explore novel antifungal agents to control the growth of this fungus. In this study, we aimed to evaluate the antifungal contact activity of two different types of carbon dots (CDS) nanomaterials; the one was coated with vitamin B12
(CDS@VB12) and the other was obtained from folic acid (FA@CDs). We used fluorescence
spectroscopy to obtain their fluorescence fingerprinting profile. The CDs were pipetted on the PDA
medium with sterilised filter paper placed in the center of the Petri dish. Then, pure A. flavus cultures were subcultured on PDA and incubated at 25°C for 7 days, and the diameters of mycelium growth were daily evaluated. The percentage of inhibition by CDS@VB12 ranged from 38.09% to 60.9% and from 46.03% to 71.39% for the amounts of 30 μl and 50 μl, respectively. On the other hand, FA@CDs did not show any inhibitory effect on the first day, and after that more progressive growth of A. flavus was noticed. The obtained results showed that CDS@VB12 could be considered as a potential new antifungal material against toxic and pathogenic A. flavus.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research",
title = "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus",
pages = "54-47",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2374"
}

Bartolić, D., Lucev, M., Stanković, M., Prokopijević, M., Algara, M., Stanković, S.,& Radotić, K.. (2023). ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research
University of Belgrade, Technical Faculty in Bor., 47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374

Bartolić D, Lucev M, Stanković M, Prokopijević M, Algara M, Stanković S, Radotić K. ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus. in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research. 2023;:47-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

Bartolić, Dragana, Lucev, Milica, Stanković, Mira, Prokopijević, Miloš, Algara, Manuel, Stanković, Slavica, Radotić, Ksenija, "ESTIMATION OF THE ANTIFUNGAL ACTIVITY OF THE TWO DIFFERENT CARBON DOTS AGAINST Aspergillus flavus" in EcoTER’23 Proceedings: 30th International Conference Ecological Truth & Enviromental Research (2023):47-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2374 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mutavdžić, Dragosav
AU  - Carstensen, Jens Michael
AU  - Stanković, Slavica
AU  - Nikolić, Milica
AU  - Krstović, Saša
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1563
AB  - Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B1
(
AFB1) from the uncontaminated seeds. The AFB1-
contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB1-
contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB1-
contaminated seeds showed a 7.9 to 9.6-fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.
PB  - Nature Research
T2  - Scientific Reports
T1  - Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study
SP  - 4849
VL  - 12
DO  - 10.1038/s41598-022-08352-4
ER  - 

@article{
author = "Bartolić, Dragana and Mutavdžić, Dragosav and Carstensen, Jens Michael and Stanković, Slavica and Nikolić, Milica and Krstović, Saša and Radotić, Ksenija",
year = "2022",
abstract = "Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B1
(
AFB1) from the uncontaminated seeds. The AFB1-
contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB1-
contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB1-
contaminated seeds showed a 7.9 to 9.6-fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study",
pages = "4849",
volume = "12",
doi = "10.1038/s41598-022-08352-4"
}

Bartolić, D., Mutavdžić, D., Carstensen, J. M., Stanković, S., Nikolić, M., Krstović, S.,& Radotić, K.. (2022). Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports
Nature Research., 12, 4849.
https://doi.org/10.1038/s41598-022-08352-4

Bartolić D, Mutavdžić D, Carstensen JM, Stanković S, Nikolić M, Krstović S, Radotić K. Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports. 2022;12:4849.
doi:10.1038/s41598-022-08352-4 .

Bartolić, Dragana, Mutavdžić, Dragosav, Carstensen, Jens Michael, Stanković, Slavica, Nikolić, Milica, Krstović, Saša, Radotić, Ksenija, "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study" in Scientific Reports, 12 (2022):4849,
https://doi.org/10.1038/s41598-022-08352-4 . .

TY  - CONF
AU  - Lucev, Milica
AU  - Savic, Iva
AU  - Radotić, Ksenija
AU  - Krnjajić, Slobodan
AU  - Bartolić, Dragana
AU  - Stevanovic, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2866
AB  - The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
T1  - ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS
EP  - 362
SP  - 362
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2866
ER  - 

@conference{
author = "Lucev, Milica and Savic, Iva and Radotić, Ksenija and Krnjajić, Slobodan and Bartolić, Dragana and Stevanovic, Milan and Stanković, Slavica",
year = "2021",
abstract = "The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"",
title = "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS",
pages = "362-362",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2866"
}

Lucev, M., Savic, I., Radotić, K., Krnjajić, S., Bartolić, D., Stevanovic, M.,& Stanković, S.. (2021). ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866

Lucev M, Savic I, Radotić K, Krnjajić S, Bartolić D, Stevanovic M, Stanković S. ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021". 2021;:362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .

Lucev, Milica, Savic, Iva, Radotić, Ksenija, Krnjajić, Slobodan, Bartolić, Dragana, Stevanovic, Milan, Stanković, Slavica, "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS" in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021" (2021):362-362,
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Mutavdžić, Dragosav
AU  - Stanković, Slavica
AU  - Jovanović, Dragoljub
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1110
AB  - In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components' positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks' positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.
PB  - Springer/Plenum Publishers, New York
T2  - Journal of Fluorescence
T1  - Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1
EP  - 733
IS  - 3
SP  - 729
VL  - 28
DO  - 10.1007/s10895-018-2246-z
ER  - 

@article{
author = "Bartolić, Dragana and Stanković, Mira and Mutavdžić, Dragosav and Stanković, Slavica and Jovanović, Dragoljub and Radotić, Ksenija",
year = "2018",
abstract = "In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components' positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks' positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Journal of Fluorescence",
title = "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1",
pages = "733-729",
number = "3",
volume = "28",
doi = "10.1007/s10895-018-2246-z"
}

Bartolić, D., Stanković, M., Mutavdžić, D., Stanković, S., Jovanović, D.,& Radotić, K.. (2018). Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1. in Journal of Fluorescence
Springer/Plenum Publishers, New York., 28(3), 729-733.
https://doi.org/10.1007/s10895-018-2246-z

Bartolić D, Stanković M, Mutavdžić D, Stanković S, Jovanović D, Radotić K. Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1. in Journal of Fluorescence. 2018;28(3):729-733.
doi:10.1007/s10895-018-2246-z .

Bartolić, Dragana, Stanković, Mira, Mutavdžić, Dragosav, Stanković, Slavica, Jovanović, Dragoljub, Radotić, Ksenija, "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1" in Journal of Fluorescence, 28, no. 3 (2018):729-733,
https://doi.org/10.1007/s10895-018-2246-z . .