TY  - CONF
AU  - Lucev, Milica
AU  - Savic, Iva
AU  - Radotić, Ksenija
AU  - Krnjajić, Slobodan
AU  - Bartolić, Dragana
AU  - Stevanovic, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2866
AB  - The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
T1  - ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS
EP  - 362
SP  - 362
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2866
ER  - 

@conference{
author = "Lucev, Milica and Savic, Iva and Radotić, Ksenija and Krnjajić, Slobodan and Bartolić, Dragana and Stevanovic, Milan and Stanković, Slavica",
year = "2021",
abstract = "The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"",
title = "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS",
pages = "362-362",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2866"
}

Lucev, M., Savic, I., Radotić, K., Krnjajić, S., Bartolić, D., Stevanovic, M.,& Stanković, S.. (2021). ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866

Lucev M, Savic I, Radotić K, Krnjajić S, Bartolić D, Stevanovic M, Stanković S. ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021". 2021;:362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .

Lucev, Milica, Savic, Iva, Radotić, Ksenija, Krnjajić, Slobodan, Bartolić, Dragana, Stevanovic, Milan, Stanković, Slavica, "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS" in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021" (2021):362-362,
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .