Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study
Аутори
Bartolić, DraganaMutavdžić, Dragosav
Carstensen, Jens Michael
Stanković, Slavica
Nikolić, Milica
Krstović, Saša
Radotić, Ksenija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B1
(
AFB1) from the uncontaminated seeds. The AFB1-
contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB1-
contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB1-
contaminated seeds showed a 7.9 to 9.6-fold increase in the seed
reflectance in the VIS region, and... 10.4 and 12.2-fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.
Кључне речи:
Aflatoxin B1 / Animals / Food Contamination / Seeds / Spectrometry / Fluorescence / Zea mays / chemistry / maize / plant seedИзвор:
Scientific Reports, 2022, 12, 4849-Издавач:
- Nature Research
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200117 (Универзитет у Новом Саду, Пољопривредни факултет) (RS-200117)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (RS-200040)
- EU Horizon 2020 Research grant no. 861915
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bartolić, Dragana AU - Mutavdžić, Dragosav AU - Carstensen, Jens Michael AU - Stanković, Slavica AU - Nikolić, Milica AU - Krstović, Saša AU - Radotić, Ksenija PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1563 AB - Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins. Besides being carcinogenic, they have other adverse health effects on humans and animals. In this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally contaminated with aflatoxin B1 ( AFB1) from the uncontaminated seeds. The AFB1- contaminated seeds exhibited a red shift of the emission maximum position compared to the control samples. Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100% was obtained to discriminate uncontaminated and AFB1- contaminated seeds. The MSI analysis combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns of the analysed seeds. The AFB1- contaminated seeds showed a 7.9 to 9.6-fold increase in the seed reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from uncontaminated seeds with high accuracy. The results may have an impact on development of spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable information for the assessment of seed adulteration in the field of food forensics and food safety. PB - Nature Research T2 - Scientific Reports T1 - Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study SP - 4849 VL - 12 DO - 10.1038/s41598-022-08352-4 ER -
@article{ author = "Bartolić, Dragana and Mutavdžić, Dragosav and Carstensen, Jens Michael and Stanković, Slavica and Nikolić, Milica and Krstović, Saša and Radotić, Ksenija", year = "2022", abstract = "Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins. Besides being carcinogenic, they have other adverse health effects on humans and animals. In this preliminary study, we used two non-invasive optical techniques, optical fiber fluorescence spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally contaminated with aflatoxin B1 ( AFB1) from the uncontaminated seeds. The AFB1- contaminated seeds exhibited a red shift of the emission maximum position compared to the control samples. Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100% was obtained to discriminate uncontaminated and AFB1- contaminated seeds. The MSI analysis combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns of the analysed seeds. The AFB1- contaminated seeds showed a 7.9 to 9.6-fold increase in the seed reflectance in the VIS region, and 10.4 and 12.2-fold increase in the NIR spectral region, compared with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from uncontaminated seeds with high accuracy. The results may have an impact on development of spectroscopic non-invasive methods for detection of AFs presence in seeds, providing valuable information for the assessment of seed adulteration in the field of food forensics and food safety.", publisher = "Nature Research", journal = "Scientific Reports", title = "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study", pages = "4849", volume = "12", doi = "10.1038/s41598-022-08352-4" }
Bartolić, D., Mutavdžić, D., Carstensen, J. M., Stanković, S., Nikolić, M., Krstović, S.,& Radotić, K.. (2022). Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports Nature Research., 12, 4849. https://doi.org/10.1038/s41598-022-08352-4
Bartolić D, Mutavdžić D, Carstensen JM, Stanković S, Nikolić M, Krstović S, Radotić K. Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports. 2022;12:4849. doi:10.1038/s41598-022-08352-4 .
Bartolić, Dragana, Mutavdžić, Dragosav, Carstensen, Jens Michael, Stanković, Slavica, Nikolić, Milica, Krstović, Saša, Radotić, Ksenija, "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study" in Scientific Reports, 12 (2022):4849, https://doi.org/10.1038/s41598-022-08352-4 . .