TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raicević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Zivin, Marko
AU  - Selih, Vid Simon
AU  - Spasojević, Ivan
AU  - Savić, Danijela Z
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1515
AB  - Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.
PB  - Wiley, Hoboken
T2  - Brain and Behavior
T1  - Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients
IS  - 2
VL  - 11
DO  - 10.1002/brb3.1986
ER  - 

@article{
author = "Opačić, Miloš and Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Raicević, Savo and Savić, Slobodan and Zorović, Maja and Zivin, Marko and Selih, Vid Simon and Spasojević, Ivan and Savić, Danijela Z",
year = "2021",
abstract = "Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.",
publisher = "Wiley, Hoboken",
journal = "Brain and Behavior",
title = "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients",
number = "2",
volume = "11",
doi = "10.1002/brb3.1986"
}

Opačić, M., Ristic, A. J., Sokic, D., Baščarević, V., Raicević, S., Savić, S., Zorović, M., Zivin, M., Selih, V. S., Spasojević, I.,& Savić, D. Z.. (2021). Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior
Wiley, Hoboken., 11(2).
https://doi.org/10.1002/brb3.1986

Opačić M, Ristic AJ, Sokic D, Baščarević V, Raicević S, Savić S, Zorović M, Zivin M, Selih VS, Spasojević I, Savić DZ. Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior. 2021;11(2).
doi:10.1002/brb3.1986 .

Opačić, Miloš, Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Raicević, Savo, Savić, Slobodan, Zorović, Maja, Zivin, Marko, Selih, Vid Simon, Spasojević, Ivan, Savić, Danijela Z, "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients" in Brain and Behavior, 11, no. 2 (2021),
https://doi.org/10.1002/brb3.1986 . .

TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Selih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2841
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.
PB  - FENS
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2841
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Nestorov, Jelena and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Selih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.",
publisher = "FENS",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2841"
}

Savic, D., Opačić, M., Nestorov, J., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Selih, V. S., Spasic, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
FENS., 46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841

Savic D, Opačić M, Nestorov J, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Zorović M, Živin M, Selih VS, Spasic S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

Savic, Danijela, Opačić, Miloš, Nestorov, Jelena, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Selih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savic, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Bascarevic, Vladimir
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2852
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - FENS
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2852
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savic, Danijela and Živin, Marko and Raičević, Savo and Bascarevic, Vladimir and Ristic, Aleksandar J and Sokic, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "FENS",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2852"
}

Opačić, M., Zorović, M., Savic, D., Živin, M., Raičević, S., Bascarevic, V., Ristic, A. J., Sokic, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
FENS., 296.
https://hdl.handle.net/21.15107/rcub_rimsi_2852

Opačić M, Zorović M, Savic D, Živin M, Raičević S, Bascarevic V, Ristic AJ, Sokic D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_rimsi_2852 .

Opačić, Miloš, Zorović, Maja, Savic, Danijela, Živin, Marko, Raičević, Savo, Bascarevic, Vladimir, Ristic, Aleksandar J, Sokic, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_rimsi_2852 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Tasić, Danica
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Bascarevic, Vladimir
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2810
AB  - Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.
PB  - Društvo biologa Srbije
C3  - Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
T1  - Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2810
ER  - 

@conference{
author = "Opačić, Miloš and Nestorov, Jelena and Tasić, Danica and Bogdanović Pristov, Jelena and Savić, Danijela Z and Bascarevic, Vladimir and Ristic, Aleksandar J and Sokic, Dragoslav and Spasojević, Ivan",
year = "2018",
abstract = "Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.",
publisher = "Društvo biologa Srbije",
journal = "Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave",
title = "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2810"
}

Opačić, M., Nestorov, J., Tasić, D., Bogdanović Pristov, J., Savić, D. Z., Bascarevic, V., Ristic, A. J., Sokic, D.,& Spasojević, I.. (2018). Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
Društvo biologa Srbije., 164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810

Opačić M, Nestorov J, Tasić D, Bogdanović Pristov J, Savić DZ, Bascarevic V, Ristic AJ, Sokic D, Spasojević I. Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave. 2018;:164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

Opačić, Miloš, Nestorov, Jelena, Tasić, Danica, Bogdanović Pristov, Jelena, Savić, Danijela Z, Bascarevic, Vladimir, Ristic, Aleksandar J, Sokic, Dragoslav, Spasojević, Ivan, "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja" in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave (2018):164,
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2961
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.
PB  - Biohemijsko društvo Srbije
C3  - Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 112
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2961
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.",
publisher = "Biohemijsko društvo Srbije",
journal = "Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "112-105",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2961"
}

Savic, D., Opačić, M., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasic, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
Biohemijsko društvo Srbije., 105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961

Savic D, Opačić M, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Živin M, Šelih VS, Spasic S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

Savic, Danijela, Opačić, Miloš, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

TY  - CHAP
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Savic, Danijela
AU  - Selih, Vid Simon
AU  - Zivin, Marko
AU  - Sokic, Dragoslav
AU  - Raičević, Savo
AU  - Bascarevic, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2938
AB  - Mesial temporal lobe epilepsy (mTLE) represents the most common subtype of human
focal epilepsies and perhaps the best-characterized disorder of this type 1
. Hippocampal
sclerosis (HS) is the most common histopathologic abnormality found in adults with drugresistant mTLE 2
. The histopathologic hallmark of HS is segmental pyramidal cell loss,
which can affect any field of the cornu Ammonis (CA1-4). Hippocampal neuronal cell loss
is always associated with a severe pattern of astrogliosis 3
. Therewithal, disturbed
homeostasis of metals is implicated in the pathology of mTLE-HS. Zinc has been
considered to play a major role in epileptogenesis in relation to its involvement in the
modulation of excitability and synaptic plasticity 4,5. Further, it has been shown that
epileptogenic hippocampi are exposed to oxidative stress and that the development of prooxidative conditions in the CNS usually involves the loss of homeostasis of iron 6,7. Low
brain levels of copper and manganese have been reported in patients with Menkes disease
and in animal models of epilepsy, and linked to seizure development 8,9. Ristić et al. were
the first to conduct a case-control study of total concentration of metals in tissue of human
HS, and report lower concentrations of copper and manganese 10.
PB  - Biohemijsko društvo Srbije
T2  - Biochemistry of Control in Life and Technology
T1  - Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 181
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2938
ER  - 

@inbook{
author = "Opačić, Miloš and Ristic, Aleksandar J and Savic, Danijela and Selih, Vid Simon and Zivin, Marko and Sokic, Dragoslav and Raičević, Savo and Bascarevic, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "Mesial temporal lobe epilepsy (mTLE) represents the most common subtype of human
focal epilepsies and perhaps the best-characterized disorder of this type 1
. Hippocampal
sclerosis (HS) is the most common histopathologic abnormality found in adults with drugresistant mTLE 2
. The histopathologic hallmark of HS is segmental pyramidal cell loss,
which can affect any field of the cornu Ammonis (CA1-4). Hippocampal neuronal cell loss
is always associated with a severe pattern of astrogliosis 3
. Therewithal, disturbed
homeostasis of metals is implicated in the pathology of mTLE-HS. Zinc has been
considered to play a major role in epileptogenesis in relation to its involvement in the
modulation of excitability and synaptic plasticity 4,5. Further, it has been shown that
epileptogenic hippocampi are exposed to oxidative stress and that the development of prooxidative conditions in the CNS usually involves the loss of homeostasis of iron 6,7. Low
brain levels of copper and manganese have been reported in patients with Menkes disease
and in animal models of epilepsy, and linked to seizure development 8,9. Ristić et al. were
the first to conduct a case-control study of total concentration of metals in tissue of human
HS, and report lower concentrations of copper and manganese 10.",
publisher = "Biohemijsko društvo Srbije",
journal = "Biochemistry of Control in Life and Technology",
booktitle = "Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "181-179",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2938"
}

Opačić, M., Ristic, A. J., Savic, D., Selih, V. S., Zivin, M., Sokic, D., Raičević, S., Bascarevic, V.,& Spasojević, I.. (2017). Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Biochemistry of Control in Life and Technology
Biohemijsko društvo Srbije., 179-181.
https://hdl.handle.net/21.15107/rcub_rimsi_2938

Opačić M, Ristic AJ, Savic D, Selih VS, Zivin M, Sokic D, Raičević S, Bascarevic V, Spasojević I. Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Biochemistry of Control in Life and Technology. 2017;:179-181.
https://hdl.handle.net/21.15107/rcub_rimsi_2938 .

Opačić, Miloš, Ristic, Aleksandar J, Savic, Danijela, Selih, Vid Simon, Zivin, Marko, Sokic, Dragoslav, Raičević, Savo, Bascarevic, Vladimir, Spasojević, Ivan, "Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Biochemistry of Control in Life and Technology (2017):179-181,
https://hdl.handle.net/21.15107/rcub_rimsi_2938 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Selih, Vid Simon
AU  - Zivin, Marko
AU  - Sokic, Dragoslav
AU  - Raicević, Savo
AU  - Baščarević, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1080
AB  - The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 148
IS  - 2
SP  - 141
VL  - 9
DO  - 10.1039/c6mt00293e
ER  - 

@article{
author = "Opačić, Miloš and Ristic, Aleksandar J and Savić, Danijela Z and Selih, Vid Simon and Zivin, Marko and Sokic, Dragoslav and Raicević, Savo and Baščarević, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "148-141",
number = "2",
volume = "9",
doi = "10.1039/c6mt00293e"
}

Opačić, M., Ristic, A. J., Savić, D. Z., Selih, V. S., Zivin, M., Sokic, D., Raicević, S., Baščarević, V.,& Spasojević, I.. (2017). Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics
Royal Soc Chemistry, Cambridge., 9(2), 141-148.
https://doi.org/10.1039/c6mt00293e

Opačić M, Ristic AJ, Savić DZ, Selih VS, Zivin M, Sokic D, Raicević S, Baščarević V, Spasojević I. Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics. 2017;9(2):141-148.
doi:10.1039/c6mt00293e .

Opačić, Miloš, Ristic, Aleksandar J, Savić, Danijela Z, Selih, Vid Simon, Zivin, Marko, Sokic, Dragoslav, Raicević, Savo, Baščarević, Vladimir, Spasojević, Ivan, "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Metallomics, 9, no. 2 (2017):141-148,
https://doi.org/10.1039/c6mt00293e . .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Sokic, Dragoslav
AU  - Bogdanović Pristov, Jelena
AU  - Nestorov, Jelena
AU  - Baščarević, Vladimir
AU  - Raicević, Savo
AU  - Savić, Slobodan
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/898
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.
PB  - Wiley, Hoboken
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
EP  - 799
IS  - 5
SP  - 789
VL  - 56
DO  - 10.1111/epi.12981
ER  - 

@article{
author = "Ristic, Aleksandar J and Savić, Danijela Z and Sokic, Dragoslav and Bogdanović Pristov, Jelena and Nestorov, Jelena and Baščarević, Vladimir and Raicević, Savo and Savić, Slobodan and Spasojević, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.",
publisher = "Wiley, Hoboken",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
pages = "799-789",
number = "5",
volume = "56",
doi = "10.1111/epi.12981"
}

Ristic, A. J., Savić, D. Z., Sokic, D., Bogdanović Pristov, J., Nestorov, J., Baščarević, V., Raicević, S., Savić, S.,& Spasojević, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia
Wiley, Hoboken., 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić DZ, Sokic D, Bogdanović Pristov J, Nestorov J, Baščarević V, Raicević S, Savić S, Spasojević I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J, Savić, Danijela Z, Sokic, Dragoslav, Bogdanović Pristov, Jelena, Nestorov, Jelena, Baščarević, Vladimir, Raicević, Savo, Savić, Slobodan, Spasojević, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .

TY  - CONF
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Spasic, Snežana D
AU  - Vojvodic, Nikola M
AU  - Savić, Slobodan
AU  - Raicević, Savo
AU  - Kovacević, Masa
AU  - Savić, Danijela Z
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/789
AB  - Altered hippocampal metallome is strongly implicated in the pathology of mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE-HS). We aimed to determine sodium, potassium, calcium, magnesium, iron, copper, manganese, and zinc concentration in epileptic human hippocampi.
PB  - Wiley-Blackwell, Hoboken
C3  - Epilepsia
T1  - Metallome of sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy
EP  - 190
SP  - 190
VL  - 55
DO  - 10.1111/epi.12675
UR  - https://hdl.handle.net/21.15107/rcub_cer_1554
ER  - 

@conference{
author = "Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Spasic, Snežana D and Vojvodic, Nikola M and Savić, Slobodan and Raicević, Savo and Kovacević, Masa and Savić, Danijela Z and Spasojević, Ivan",
year = "2014",
abstract = "Altered hippocampal metallome is strongly implicated in the pathology of mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE-HS). We aimed to determine sodium, potassium, calcium, magnesium, iron, copper, manganese, and zinc concentration in epileptic human hippocampi.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Metallome of sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy",
pages = "190-190",
volume = "55",
doi = "10.1111/epi.12675",
url = "https://hdl.handle.net/21.15107/rcub_cer_1554"
}

Ristic, A. J., Sokic, D., Baščarević, V., Spasic, S. D., Vojvodic, N. M., Savić, S., Raicević, S., Kovacević, M., Savić, D. Z.,& Spasojević, I.. (2014). Metallome of sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia
Wiley-Blackwell, Hoboken., 55, 190-190.
https://doi.org/10.1111/epi.12675
https://hdl.handle.net/21.15107/rcub_cer_1554

Ristic AJ, Sokic D, Baščarević V, Spasic SD, Vojvodic NM, Savić S, Raicević S, Kovacević M, Savić DZ, Spasojević I. Metallome of sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55:190-190.
doi:10.1111/epi.12675
https://hdl.handle.net/21.15107/rcub_cer_1554 .

Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Spasic, Snežana D, Vojvodic, Nikola M, Savić, Slobodan, Raicević, Savo, Kovacević, Masa, Savić, Danijela Z, Spasojević, Ivan, "Metallome of sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55 (2014):190-190,
https://doi.org/10.1111/epi.12675 .,
https://hdl.handle.net/21.15107/rcub_cer_1554 .

TY  - CONF
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bogdanović Pristov, Jelena
AU  - Baščarević, Vladimir
AU  - Nestorov, Jelena
AU  - Vojvodic, Nikola M
AU  - Kovacević, Masa
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/788
PB  - Wiley-Blackwell, Hoboken
C3  - Epilepsia
T1  - Increased activity of hydrogen peroxide-removing enzymes in hippocampi of the patients with mesial temporal lobe epilepsy due to hippocampal sclerosis
EP  - 189
SP  - 189
VL  - 55
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_788
ER  - 

@conference{
author = "Ristic, Aleksandar J and Sokic, Dragoslav and Bogdanović Pristov, Jelena and Baščarević, Vladimir and Nestorov, Jelena and Vojvodic, Nikola M and Kovacević, Masa and Spasojević, Ivan",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Increased activity of hydrogen peroxide-removing enzymes in hippocampi of the patients with mesial temporal lobe epilepsy due to hippocampal sclerosis",
pages = "189-189",
volume = "55",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_788"
}

Ristic, A. J., Sokic, D., Bogdanović Pristov, J., Baščarević, V., Nestorov, J., Vojvodic, N. M., Kovacević, M.,& Spasojević, I.. (2014). Increased activity of hydrogen peroxide-removing enzymes in hippocampi of the patients with mesial temporal lobe epilepsy due to hippocampal sclerosis. in Epilepsia
Wiley-Blackwell, Hoboken., 55, 189-189.
https://hdl.handle.net/21.15107/rcub_rimsi_788

Ristic AJ, Sokic D, Bogdanović Pristov J, Baščarević V, Nestorov J, Vojvodic NM, Kovacević M, Spasojević I. Increased activity of hydrogen peroxide-removing enzymes in hippocampi of the patients with mesial temporal lobe epilepsy due to hippocampal sclerosis. in Epilepsia. 2014;55:189-189.
https://hdl.handle.net/21.15107/rcub_rimsi_788 .

Ristic, Aleksandar J, Sokic, Dragoslav, Bogdanović Pristov, Jelena, Baščarević, Vladimir, Nestorov, Jelena, Vojvodic, Nikola M, Kovacević, Masa, Spasojević, Ivan, "Increased activity of hydrogen peroxide-removing enzymes in hippocampi of the patients with mesial temporal lobe epilepsy due to hippocampal sclerosis" in Epilepsia, 55 (2014):189-189,
https://hdl.handle.net/21.15107/rcub_rimsi_788 .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Spasic, Snežana D
AU  - Vojvodic, Nikola M
AU  - Savić, Slobodan
AU  - Raicević, Savo
AU  - Kovacević, Masa
AU  - Savić, Danijela Z
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/791
AB  - An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .
PB  - Wiley-Blackwell, Hoboken
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy
EP  - E37
IS  - 5
SP  - E34
VL  - 55
DO  - 10.1111/epi.12593
ER  - 

@article{
author = "Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Spasic, Snežana D and Vojvodic, Nikola M and Savić, Slobodan and Raicević, Savo and Kovacević, Masa and Savić, Danijela Z and Spasojević, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy",
pages = "E37-E34",
number = "5",
volume = "55",
doi = "10.1111/epi.12593"
}

Ristic, A. J., Sokic, D., Baščarević, V., Spasic, S. D., Vojvodic, N. M., Savić, S., Raicević, S., Kovacević, M., Savić, D. Z.,& Spasojević, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia
Wiley-Blackwell, Hoboken., 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Baščarević V, Spasic SD, Vojvodic NM, Savić S, Raicević S, Kovacević M, Savić DZ, Spasojević I. Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Spasic, Snežana D, Vojvodic, Nikola M, Savić, Slobodan, Raicević, Savo, Kovacević, Masa, Savić, Danijela Z, Spasojević, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .