TY  - JOUR
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajic, Tanja
AU  - Ciric, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Todorović, Nataša
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1082
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
EP  - 372
IS  - 3
SP  - 364
VL  - 163
DO  - 10.1099/mic.0.000429
ER  - 

@article{
author = "Stanić, Marina and Krizak, Strahinja and Jovanović, Mirna and Pajic, Tanja and Ciric, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Todorović, Nataša and Zivić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
pages = "372-364",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429"
}

Stanić, M., Krizak, S., Jovanović, M., Pajic, T., Ciric, A., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Todorović, N.,& Zivić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm
Microbiology Soc, London., 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Krizak S, Jovanović M, Pajic T, Ciric A, Žižić M, Zakrzewska J, Cvetić-Antić T, Todorović N, Zivić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marina, Krizak, Strahinja, Jovanović, Mirna, Pajic, Tanja, Ciric, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Todorović, Nataša, Zivić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology-Sgm, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikolić, Ljiljana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/869
AB  - We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
EP  - 173
IS  - 3
SP  - 162
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
ER  - 

@article{
author = "Krizak, Strahinja and Nikolić, Ljiljana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores",
pages = "173-162",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004"
}

Krizak, S., Nikolić, L., Stanić, M., Žižić, M., Zakrzewska, J., Zivić, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology
Elsevier, Amsterdam., 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikolić L, Stanić M, Žižić M, Zakrzewska J, Zivić M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikolić, Ljiljana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Ducic, Tanja
AU  - Grolimund, Daniel
AU  - Bajuk-Bogdanović, Danica
AU  - Nikolic, Miroslav
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Zakrzewska, Joanna
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/862
AB  - Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium
EP  - 7496
IS  - 24
SP  - 7487
VL  - 407
DO  - 10.1007/s00216-015-8916-7
ER  - 

@article{
author = "Žižić, Milan and Ducic, Tanja and Grolimund, Daniel and Bajuk-Bogdanović, Danica and Nikolic, Miroslav and Stanić, Marina and Krizak, Strahinja and Zakrzewska, Joanna",
year = "2015",
abstract = "Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium",
pages = "7496-7487",
number = "24",
volume = "407",
doi = "10.1007/s00216-015-8916-7"
}

Žižić, M., Ducic, T., Grolimund, D., Bajuk-Bogdanović, D., Nikolic, M., Stanić, M., Krizak, S.,& Zakrzewska, J.. (2015). X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 407(24), 7487-7496.
https://doi.org/10.1007/s00216-015-8916-7

Žižić M, Ducic T, Grolimund D, Bajuk-Bogdanović D, Nikolic M, Stanić M, Krizak S, Zakrzewska J. X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry. 2015;407(24):7487-7496.
doi:10.1007/s00216-015-8916-7 .

Žižić, Milan, Ducic, Tanja, Grolimund, Daniel, Bajuk-Bogdanović, Danica, Nikolic, Miroslav, Stanić, Marina, Krizak, Strahinja, Zakrzewska, Joanna, "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium" in Analytical and Bioanalytical Chemistry, 407, no. 24 (2015):7487-7496,
https://doi.org/10.1007/s00216-015-8916-7 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta
AU  - Pajdic, Aleksandra
AU  - Krizak, Strahinja
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/809
AB  - The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus
EP  - 857
IS  - 2
SP  - 847
VL  - 66
DO  - 10.2298/ABS1402847S
ER  - 

@article{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta and Pajdic, Aleksandra and Krizak, Strahinja and Žižić, Milan and Zakrzewska, Joanna",
year = "2014",
abstract = "The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus",
pages = "857-847",
number = "2",
volume = "66",
doi = "10.2298/ABS1402847S"
}

Stanić, M., Zivić, M., Hadzibrahimović, M., Pajdic, A., Krizak, S., Žižić, M.,& Zakrzewska, J.. (2014). Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 847-857.
https://doi.org/10.2298/ABS1402847S

Stanić M, Zivić M, Hadzibrahimović M, Pajdic A, Krizak S, Žižić M, Zakrzewska J. Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences. 2014;66(2):847-857.
doi:10.2298/ABS1402847S .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta, Pajdic, Aleksandra, Krizak, Strahinja, Žižić, Milan, Zakrzewska, Joanna, "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus" in Archives of Biological Sciences, 66, no. 2 (2014):847-857,
https://doi.org/10.2298/ABS1402847S . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Maksimović, Vuk
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/750
AB  - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
IS  - 7
VL  - 9
DO  - 10.1371/journal.pone.0102849
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2014",
abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus",
number = "7",
volume = "9",
doi = "10.1371/journal.pone.0102849"
}

Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One
Public Library Science, San Francisco., 9(7).
https://doi.org/10.1371/journal.pone.0102849

Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7).
doi:10.1371/journal.pone.0102849 .

Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014),
https://doi.org/10.1371/journal.pone.0102849 . .