dc.creator | Jelušić, Aleksandra | |
dc.creator | Berić, Tanja | |
dc.creator | Mitrović, Petar | |
dc.creator | Marković, Sanja | |
dc.creator | Stanković, Slaviša | |
dc.creator | Popović, Tatjana | |
dc.date.accessioned | 2023-05-18T07:53:13Z | |
dc.date.available | 2023-05-18T07:53:13Z | |
dc.date.issued | 2019 | |
dc.identifier.isbn | 978-86-87109-15-5 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/1912 | |
dc.description.abstract | Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc)
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from
different localities in Serbia, with the aim to examine their genetic features, through
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained
sequences were checked for homology with strains available in NCBI database. Multilocus
sequence analysis (MLSA) was then performed to determine relatedness among the tested
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity. | sr |
dc.language.iso | en | sr |
dc.publisher | Serbian Genetic Society, Belgrade, Serbia | sr |
dc.relation | Ministry of Education, Science and Technological Development, Republic of Serbia (Project No. III43010) | sr |
dc.rights | openAccess | sr |
dc.source | 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY | sr |
dc.subject | oilseed rape | sr |
dc.subject | Xanthomonas | sr |
dc.subject | bacterial blight | sr |
dc.subject | multilocus sequence analysis (mlsa) | sr |
dc.title | GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.spage | 163 | |
dc.identifier.fulltext | http://rimsi.imsi.bg.ac.rs/bitstream/id/4992/bitstream_4992.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_rimsi_1912 | |
dc.type.version | publishedVersion | sr |