TY  - JOUR
AU  - Pejin, Boris
AU  - Simonović, Mladen
AU  - Talevska, Aleksandra
AU  - Glumac, Miodrag
AU  - Jakimov, Dimitar
AU  - Kojić, Vesna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1511
AB  - The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A neglected natural source for targeting glioblastoma
EP  - 1860
IS  - 11
SP  - 1856
VL  - 35
DO  - 10.1080/14786419.2019.1638386
ER  - 

@article{
author = "Pejin, Boris and Simonović, Mladen and Talevska, Aleksandra and Glumac, Miodrag and Jakimov, Dimitar and Kojić, Vesna",
year = "2021",
abstract = "The cytotoxicity of the methanol extract of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) (Malawispongiidae) was evaluated by MTT assay at in vitro conditions against three brain tumour cell lines (Neuro-2A, U-251 MG and U-87 MG). The extract was actually found to be most effective against the malignant glioma U-251 MG cells reaching a promising IC50 value of 1.87 +/- 0.09 mu g/mL at 96 h. However, it exhibited only a bit of cytotoxicity (IC50 321.14 +/- 11.29 mu g/mL, 96 h) towards the normal cells. Also, this sponge extract was 5-fold more selective for U-251 MG versus U-87 MG cells. Finally, monitoring genotoxicity at chromosomal level using the micronucleus test practically revealed lack of any significant toxicity of O. rotunda extract, compared to doxorubicin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A neglected natural source for targeting glioblastoma",
pages = "1860-1856",
number = "11",
volume = "35",
doi = "10.1080/14786419.2019.1638386"
}

Pejin, B., Simonović, M., Talevska, A., Glumac, M., Jakimov, D.,& Kojić, V.. (2021). A neglected natural source for targeting glioblastoma. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 35(11), 1856-1860.
https://doi.org/10.1080/14786419.2019.1638386

Pejin B, Simonović M, Talevska A, Glumac M, Jakimov D, Kojić V. A neglected natural source for targeting glioblastoma. in Natural Product Research. 2021;35(11):1856-1860.
doi:10.1080/14786419.2019.1638386 .

Pejin, Boris, Simonović, Mladen, Talevska, Aleksandra, Glumac, Miodrag, Jakimov, Dimitar, Kojić, Vesna, "A neglected natural source for targeting glioblastoma" in Natural Product Research, 35, no. 11 (2021):1856-1860,
https://doi.org/10.1080/14786419.2019.1638386 . .

TY  - JOUR
AU  - Talevska, Aleksandra
AU  - Pejin, Boris
AU  - Kojić, Vesna
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1149
AB  - In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A contribution to pharmaceutical biology of freshwater sponges
EP  - 571
IS  - 5
SP  - 568
VL  - 32
DO  - 10.1080/14786419.2017.1315719
ER  - 

@article{
author = "Talevska, Aleksandra and Pejin, Boris and Kojić, Vesna and Berić, Tanja and Stanković, Slaviša",
year = "2018",
abstract = "In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 +/- 0.21 mu g/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 +/- 0.13 mu g/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold). Its profound anti-DPPH radical activity comparable to that of quercetin (IC50 values 3.68 +/- 0.19 and 3.14 +/- 0.09 mu g/mL, respectively) coupled with no signs of genotoxicity in the comet assay (MRC-5 cell line, vs. doxorubicin) has actually implicated the importance of this animal bioresource in searching for pharmaceutically useful bioactive compounds of natural origin. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A contribution to pharmaceutical biology of freshwater sponges",
pages = "571-568",
number = "5",
volume = "32",
doi = "10.1080/14786419.2017.1315719"
}

Talevska, A., Pejin, B., Kojić, V., Berić, T.,& Stanković, S.. (2018). A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 32(5), 568-571.
https://doi.org/10.1080/14786419.2017.1315719

Talevska A, Pejin B, Kojić V, Berić T, Stanković S. A contribution to pharmaceutical biology of freshwater sponges. in Natural Product Research. 2018;32(5):568-571.
doi:10.1080/14786419.2017.1315719 .

Talevska, Aleksandra, Pejin, Boris, Kojić, Vesna, Berić, Tanja, Stanković, Slaviša, "A contribution to pharmaceutical biology of freshwater sponges" in Natural Product Research, 32, no. 5 (2018):568-571,
https://doi.org/10.1080/14786419.2017.1315719 . .

TY  - JOUR
AU  - Talevska, Aleksandra
AU  - Pejin, Boris
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1065
AB  - Context: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. Objective: This work focuses on the screening of acetylcholinesterase inhibitory (AChE) and mutagenic activities of the acetone extract (obtained by maceration) of the freshwater sponge Ochridaspongia rotunda Arndt (Malawispongiidae) in vitro. Materials and methods: AChE inhibitory activity was evaluated both in liquid (five different concentrations of the extract, from 1 to 100 mu g/mL) and in solid (seven different concentrations of the extract, from 0.5 to 10.0 mu g) by methods well described in literature, while mutagenicity was estimated using the Ames test (four different concentrations of the extract, from 0.106 to 1.328mg/plate). Results: Ochridaspongia rotunda acetone extract exhibited promising AChE inhibitory activity in a dose-dependent manner both in liquid (IC50 23.07 mu g/mL) and in solid (1.50 mu g). Furthermore, the Ames test revealed no sign of mutagenicity at any concentration tested. Its FTIR spectrum coupled with the positive Liebermann-Burchard, Salkowski and Zak color reactions (tests) indicated the presence of sterol compounds. Discussion and conclusion: The screened extract may inspire a search for novel anticholinesterase therapeutic agent(s) potentially used in the treatment of Alzheimer's disease. Further research will be directed toward its detailed chemical analysis along with addressing the issue of a real producer of the natural product(s) responsible for the AChE activity observed.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Biology
T1  - Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda
EP  - 1316
IS  - 1
SP  - 1313
VL  - 55
DO  - 10.1080/13880209.2017.1297468
ER  - 

@article{
author = "Talevska, Aleksandra and Pejin, Boris and Berić, Tanja and Stanković, Slaviša",
year = "2017",
abstract = "Context: Bioprospection has become a dynamic scientific field that explores novel possibilities for the implementation of natural products in medicine and pharmacy. Compared to marine species from all kingdoms, freshwater species have been highly neglected. Objective: This work focuses on the screening of acetylcholinesterase inhibitory (AChE) and mutagenic activities of the acetone extract (obtained by maceration) of the freshwater sponge Ochridaspongia rotunda Arndt (Malawispongiidae) in vitro. Materials and methods: AChE inhibitory activity was evaluated both in liquid (five different concentrations of the extract, from 1 to 100 mu g/mL) and in solid (seven different concentrations of the extract, from 0.5 to 10.0 mu g) by methods well described in literature, while mutagenicity was estimated using the Ames test (four different concentrations of the extract, from 0.106 to 1.328mg/plate). Results: Ochridaspongia rotunda acetone extract exhibited promising AChE inhibitory activity in a dose-dependent manner both in liquid (IC50 23.07 mu g/mL) and in solid (1.50 mu g). Furthermore, the Ames test revealed no sign of mutagenicity at any concentration tested. Its FTIR spectrum coupled with the positive Liebermann-Burchard, Salkowski and Zak color reactions (tests) indicated the presence of sterol compounds. Discussion and conclusion: The screened extract may inspire a search for novel anticholinesterase therapeutic agent(s) potentially used in the treatment of Alzheimer's disease. Further research will be directed toward its detailed chemical analysis along with addressing the issue of a real producer of the natural product(s) responsible for the AChE activity observed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Biology",
title = "Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda",
pages = "1316-1313",
number = "1",
volume = "55",
doi = "10.1080/13880209.2017.1297468"
}

Talevska, A., Pejin, B., Berić, T.,& Stanković, S.. (2017). Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda. in Pharmaceutical Biology
Taylor & Francis Ltd, Abingdon., 55(1), 1313-1316.
https://doi.org/10.1080/13880209.2017.1297468

Talevska A, Pejin B, Berić T, Stanković S. Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda. in Pharmaceutical Biology. 2017;55(1):1313-1316.
doi:10.1080/13880209.2017.1297468 .

Talevska, Aleksandra, Pejin, Boris, Berić, Tanja, Stanković, Slaviša, "Further insight into the bioactivity of the freshwater sponge Ochridaspongia rotunda" in Pharmaceutical Biology, 55, no. 1 (2017):1313-1316,
https://doi.org/10.1080/13880209.2017.1297468 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Talevska, Aleksandra
AU  - Ciric, Ana
AU  - Glamočlija, Jasmina
AU  - Nikolic, Milos
AU  - Talevski, Trajce
AU  - Soković, Marina
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/806
AB  - The anti-quorum sensing activities towards the bacterium Pseudomonas aeruginosa PA01 (pyocyanin production, biofilm formation and twitching and flagella motility) of two crude extracts (methanol and acetone) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) were evaluated in vitro for the first time. Both extracts demonstrated P. aeruginosa pyocyanin inhibitory activity, reducing its production for 49.90% and 42.44%, respectively. In addition, they both showed higher anti-biofilm activity (48.29% and 53.99%, respectively) than ampicillin (30.84%). Finally, O. rotunda extracts effectively reduced twitching and flagella motility of P. aeruginosa. Taken all together, these results suggest that endemic sponge species from the oldest lake in Europe may offer novel bioactive natural products with promising medicinal potential towards P. aeruginosa infections.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa
EP  - 2333
IS  - 24
SP  - 2330
VL  - 28
DO  - 10.1080/14786419.2014.934239
ER  - 

@article{
author = "Pejin, Boris and Talevska, Aleksandra and Ciric, Ana and Glamočlija, Jasmina and Nikolic, Milos and Talevski, Trajce and Soković, Marina",
year = "2014",
abstract = "The anti-quorum sensing activities towards the bacterium Pseudomonas aeruginosa PA01 (pyocyanin production, biofilm formation and twitching and flagella motility) of two crude extracts (methanol and acetone) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) were evaluated in vitro for the first time. Both extracts demonstrated P. aeruginosa pyocyanin inhibitory activity, reducing its production for 49.90% and 42.44%, respectively. In addition, they both showed higher anti-biofilm activity (48.29% and 53.99%, respectively) than ampicillin (30.84%). Finally, O. rotunda extracts effectively reduced twitching and flagella motility of P. aeruginosa. Taken all together, these results suggest that endemic sponge species from the oldest lake in Europe may offer novel bioactive natural products with promising medicinal potential towards P. aeruginosa infections.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa",
pages = "2333-2330",
number = "24",
volume = "28",
doi = "10.1080/14786419.2014.934239"
}

Pejin, B., Talevska, A., Ciric, A., Glamočlija, J., Nikolic, M., Talevski, T.,& Soković, M.. (2014). Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(24), 2330-2333.
https://doi.org/10.1080/14786419.2014.934239

Pejin B, Talevska A, Ciric A, Glamočlija J, Nikolic M, Talevski T, Soković M. Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa. in Natural Product Research. 2014;28(24):2330-2333.
doi:10.1080/14786419.2014.934239 .

Pejin, Boris, Talevska, Aleksandra, Ciric, Ana, Glamočlija, Jasmina, Nikolic, Milos, Talevski, Trajce, Soković, Marina, "Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa" in Natural Product Research, 28, no. 24 (2014):2330-2333,
https://doi.org/10.1080/14786419.2014.934239 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Talevska, Aleksandra
AU  - Ciric, Ana
AU  - Glamočlija, Jasmina
AU  - Nikolic, Milos
AU  - Talevski, Trajce
AU  - Soković, Marina
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/784
AB  - The antimicrobial activity of five crude extracts (aqueous, methanol, ethyl acetate, acetone and methylene chloride) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) was evaluated in vitro by using microdilution method against eight bacterial and eight fungal strains for the first time. The extracts were proven to be active in varying degrees against all the bacteria and fungi tested. O. rotunda methanol extract exhibited the highest antibacterial activity (minimum inhibitory concentration (MIC) 7.5-15.0 mu g/mL and minimum bactericidal concentration 15-30 mu g/mL), while its acetone extract exhibited the most promising antifungal activity (MIC 7.5-45.0 mu g/mL and minimum fungicidal concentration 15-60 mu g/mL). The extracts were more effective against the bacteria and fungi screened compared with the positive controls (streptomycin and ampicillin for bacteria and bifonazole and ketoconazole for fungi, respectively). According to the experimental data obtained, this deepwater sponge species may be considered as a gold mine of new antimicrobial substances with significant and broad-range activity.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)
EP  - 1494
IS  - 18
SP  - 1489
VL  - 28
DO  - 10.1080/14786419.2014.911297
ER  - 

@article{
author = "Pejin, Boris and Talevska, Aleksandra and Ciric, Ana and Glamočlija, Jasmina and Nikolic, Milos and Talevski, Trajce and Soković, Marina",
year = "2014",
abstract = "The antimicrobial activity of five crude extracts (aqueous, methanol, ethyl acetate, acetone and methylene chloride) of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937) was evaluated in vitro by using microdilution method against eight bacterial and eight fungal strains for the first time. The extracts were proven to be active in varying degrees against all the bacteria and fungi tested. O. rotunda methanol extract exhibited the highest antibacterial activity (minimum inhibitory concentration (MIC) 7.5-15.0 mu g/mL and minimum bactericidal concentration 15-30 mu g/mL), while its acetone extract exhibited the most promising antifungal activity (MIC 7.5-45.0 mu g/mL and minimum fungicidal concentration 15-60 mu g/mL). The extracts were more effective against the bacteria and fungi screened compared with the positive controls (streptomycin and ampicillin for bacteria and bifonazole and ketoconazole for fungi, respectively). According to the experimental data obtained, this deepwater sponge species may be considered as a gold mine of new antimicrobial substances with significant and broad-range activity.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)",
pages = "1494-1489",
number = "18",
volume = "28",
doi = "10.1080/14786419.2014.911297"
}

Pejin, B., Talevska, A., Ciric, A., Glamočlija, J., Nikolic, M., Talevski, T.,& Soković, M.. (2014). In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937). in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(18), 1489-1494.
https://doi.org/10.1080/14786419.2014.911297

Pejin B, Talevska A, Ciric A, Glamočlija J, Nikolic M, Talevski T, Soković M. In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937). in Natural Product Research. 2014;28(18):1489-1494.
doi:10.1080/14786419.2014.911297 .

Pejin, Boris, Talevska, Aleksandra, Ciric, Ana, Glamočlija, Jasmina, Nikolic, Milos, Talevski, Trajce, Soković, Marina, "In vitro evaluation of antimicrobial activity of the freshwater sponge Ochridaspongia rotunda (Arndt, 1937)" in Natural Product Research, 28, no. 18 (2014):1489-1494,
https://doi.org/10.1080/14786419.2014.911297 . .